Genus: Species: E. histolytica E. dispar E. hartmanni E. coli E. gingivalis E. Polecki E. moshkovskii Genus: Species: E. nana Genus: Iodamoeba Species: I. bütschlii

(Peripheral Chromatin)

(linin network) (Pseudopodia) Binary fission

E. coli 15-50 µ

10-35 µ E. coli E. histolytica

T:12-60 µ

C:10-15 µ

Minuta :12- 15 µ

Trophozoites Cysts E. histolytica E. histolytica Hematophage Magnata E. dispar E. histolytica /E. dispar

E. moshkovskii

E. histolytica /E. dispar/E. moshkovskii E. hartmanni

T:10-12 µ

C:7-10 µ E. gingivalis

T: 10 - 35 µ E. Polecki T:25-30 µ C:10-15 µ Endolimax nana

T:5-15 µ T:6-8 µ

C:6-8 µ Iodamoeba bütschlii T:12-16 µ

C:9-10 µ Genus: Entamoeba

Species: E. histolytica Amebiasis E. dispar E. hartmanni E. Polecki E. coli E. moshkovskii -

– 

( Anal sex ) 

 ( Fecal-oral self-inoculation ) .I .II .I     

    .II 

E. dispar E. moshkovskii

(Immunodiagnosis) 1. Antibody Detection IHA , IFA , ELISA

2. Antigen Detection ELISA

)Molecular diagnosis ( E. HISTOLYTICA II TECHLAB® A 2nd generation Monoclonal ELISA for detecting E. histolytica adhesin in fecal specimens Catalog No. T5017 (96 Tests) E. dispar E. moshkovskii

(Immunodiagnosis) 1. Antibody Detection IHA , IFA , ELISA

2. Antigen Detection ELISA

)Molecular diagnosis ( PCR

Phase Contrast Microscopy X 400 Entamoeba coli uninucleate cyst cyst

Phase Contrast Microscopy X 400

Entamoeba histolytica Macrophage trophozoite Phase Contrast Microscopy X 400 Entamoeba histolytica Epithelial cells trophozoites Phase Contrast Microscopy X 400

Entamoeba histolytica Entamoeba coli trophozoite Phase Contrast Microscopy X 400 Entamoeba histolytica Polymorphonuclear cyst leucocytes Trichrome Stain x 1000

Entamoeba histolytica Macrophage cyst

PH PH ! ! ! ! ! !

  

Naegleria Balamuthia fowleri Primary Amebic Meingoencephalitis (PAM)

( lobopodia )  10-35 µm   Amebostome  

(9 µm )8-12 µ

Thermophilic trophozoite Naegleria fowleri trophozoite in spinal fluid. Trichrome stain. Note the typically large karyosome and the monopodial locomotion. primary amebic meingoencephalitis (PAM)

B + B Acanthamoeba spp ostiole Granulomatous Amebic Encephalitis (GAE) ulcerating keratitis

10-45 µm

( Acantapodia )

7-18 µm

-2 → 45 °C 35-25°C Acanthamoeba sp. Notice the typical thorn-like projections (acanthopoda).

Acanthamoeba spp

Granulomatous Amebic Encephalitis (GAE) Acanthamoeba castellanii A. culbertsoni A. astronyxis A. palestinensis A. polyphaga A. hatchetti A. rhysodes A. griffini Balamuthia mandrillaris Granulomatous Amebic Encephalitis (GAE)

Acanthamoeba and B. mandrillaris Infections of the Lungs and Skin Acanthamoebic pneumonitis and dermatitis

Acanthamoeba Keratitis ulcerating keratitis Pathogenesis of Acanthamoeba infection

Acanthamoeba Keratitis Brolene (propamidine isethionate) dibromopropamidine

Polymyxin B miconazole + propamidine neomycin

Ketoconazole Acridine derivatives paromomycin Acanthamoeba sp. Keratitis

Corneal biopsy showing a trophozoite

Cyst with the characteristic stellate shape, in corneal scraping. Balamuthia mandrillaris Leptomyxid ameba

Meningoencephalitis Balamuthia amebic encephalitis (BAE)

( 30 ) 12-60 µm

Balamuthia mandrillaris ( 15 ) 6 - 30 µm trophozoites in brain tissue Immunofluorescent staining

Balamuthia mandrillaris

30 µ.

Balamuthia mandrillaris infection with important skin lesion.