Entamoeba Histolytica Uninucleate Cyst Cyst

Entamoeba Histolytica Uninucleate Cyst Cyst

Genus: Entamoeba Species: E. histolytica E. dispar E. hartmanni E. coli E. gingivalis E. Polecki E. moshkovskii Genus: Endolimax Species: E. nana Genus: Iodamoeba Species: I. bütschlii (Peripheral Chromatin) (linin network) (Pseudopodia) Binary fission E. coli 15-50 µ 10-35 µ E. coli E. histolytica T:12-60 µ C:10-15 µ Minuta :12- 15 µ Trophozoites Cysts E. histolytica E. histolytica Hematophage Magnata E. dispar E. histolytica /E. dispar E. moshkovskii E. histolytica /E. dispar/E. moshkovskii E. hartmanni T:10-12 µ C:7-10 µ E. gingivalis T: 10 - 35 µ E. Polecki T:25-30 µ C:10-15 µ Endolimax nana T:5-15 µ T:6-8 µ C:6-8 µ Iodamoeba bütschlii T:12-16 µ C:9-10 µ Genus: Entamoeba Species: E. histolytica Amebiasis E. dispar E. hartmanni E. Polecki E. coli E. moshkovskii - – ( Anal sex ) ( Fecal-oral self-inoculation ) .I .II .I .II E. dispar E. moshkovskii (Immunodiagnosis) 1. Antibody Detection IHA , IFA , ELISA 2. Antigen Detection ELISA )Molecular diagnosis ( E. HISTOLYTICA II TECHLAB® A 2nd generation Monoclonal ELISA for detecting E. histolytica adhesin in fecal specimens Catalog No. T5017 (96 Tests) E. dispar E. moshkovskii (Immunodiagnosis) 1. Antibody Detection IHA , IFA , ELISA 2. Antigen Detection ELISA )Molecular diagnosis ( PCR Phase Contrast Microscopy X 400 Entamoeba coli Entamoeba histolytica uninucleate cyst cyst Phase Contrast Microscopy X 400 Entamoeba histolytica Macrophage trophozoite Phase Contrast Microscopy X 400 Entamoeba histolytica Epithelial cells trophozoites Phase Contrast Microscopy X 400 Entamoeba histolytica Entamoeba coli trophozoite Phase Contrast Microscopy X 400 Entamoeba histolytica Polymorphonuclear cyst leucocytes Trichrome Stain x 1000 Entamoeba histolytica Macrophage cyst PH PH ! ! ! ! ! ! Naegleria Acanthamoeba Balamuthia Naegleria fowleri Primary Amebic Meingoencephalitis (PAM) ( lobopodia ) 10-35 µm Amebostome (9 µm )8-12 µ Thermophilic Naegleria fowleri trophozoite Naegleria fowleri trophozoite in spinal fluid. Trichrome stain. Note the typically large karyosome and the monopodial locomotion. primary amebic meingoencephalitis (PAM) B + B Acanthamoeba spp ostiole Granulomatous Amebic Encephalitis (GAE) ulcerating keratitis 10-45 µm ( Acantapodia ) 7-18 µm -2 → 45 °C 35-25°C Acanthamoeba sp. Notice the typical thorn-like projections (acanthopoda). Acanthamoeba spp Granulomatous Amebic Encephalitis (GAE) Acanthamoeba castellanii A. culbertsoni A. astronyxis A. palestinensis A. polyphaga A. hatchetti A. rhysodes A. griffini Balamuthia mandrillaris Granulomatous Amebic Encephalitis (GAE) Acanthamoeba and B. mandrillaris Infections of the Lungs and Skin Acanthamoebic pneumonitis and dermatitis Acanthamoeba Keratitis ulcerating keratitis Pathogenesis of Acanthamoeba infection Acanthamoeba Keratitis Brolene (propamidine isethionate) dibromopropamidine Polymyxin B miconazole + propamidine neomycin Ketoconazole Acridine derivatives paromomycin Acanthamoeba sp. Keratitis Corneal biopsy showing a trophozoite Cyst with the characteristic stellate shape, in corneal scraping. Balamuthia mandrillaris Leptomyxid ameba Meningoencephalitis Balamuthia amebic encephalitis (BAE) ( 30 ) 12-60 µm Balamuthia mandrillaris ( 15 ) 6 - 30 µm trophozoites in brain tissue Immunofluorescent staining Balamuthia mandrillaris 30 µ. Balamuthia mandrillaris infection with important skin lesion. .

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