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The Use of the Hypervariable P8 Region of Trnl (UAA) Intron for Identification of Orchid Species: Evidence from Restriction Site Polymorphism Analysis
RESEARCH ARTICLE The use of the hypervariable P8 region of trnL (UAA) intron for identification of orchid species: Evidence from restriction site polymorphism analysis Rajkumar Kishor¤*, G. J. Sharma Department of Life Sciences, Manipur University, Imphal, Manipur, India ¤ Current address: Kwaklei and Khonggunmelei Orchids Pvt. Ltd., Sagolband Vijaygovind, Imphal, Manipur, a1111111111 India a1111111111 * [email protected] a1111111111 a1111111111 a1111111111 Abstract The P8 stem-loop region of the trnL intron, which is known to be hypervariable in size with multiple repeat motifs and created difficulties in alignment, is always excluded in phyloge- netic as well as barcode analyses. This region was investigated for species discrimination in OPEN ACCESS 98 taxa of orchids belonging to the tribe Vandeae using in silico mapping of restriction site Citation: Kishor R, Sharma GJ (2018) The use of polymorphism. The length of the P8 regions varied from 200 nucleotides in Aerides rosea to the hypervariable P8 region of trnL(UAA) intron for identification of orchid species: Evidence from 669 nucleotides in Dendrophylax sallei. Forty two taxa had unique lengths, while as many restriction site polymorphism analysis. PLoS ONE as eight shared a common length of 521 nucleotides. Of the 35 restriction endonucleases 13(5): e0196680. https://doi.org/10.1371/journal. producing digestions in the P8 regions, three, viz., AgsI, ApoI and TspDTI turned out to pone.0196680 have recognition sites across all the 98 taxa being studied. When their restriction data were Editor: Serena Aceto, University of Naples Federico combined, 92 taxa could be discriminated leaving three taxon pairs. However, Acampe II, ITALY papillosa and Aeranthes arachnites despite having similar restriction sites differed in their Received: January 17, 2018 P8 lengths. -
Keunggulan Kelompok Anggrek Vanda Dalam Meningkatkan Variasi Dan Kualitas Anggrek Bunga Potong
Prosiding Seminar Nasional Anggrek 2012 Makalah Pendukung 4 Keunggulan Kelompok Anggrek Vanda dalam Meningkatkan Variasi dan Kualitas Anggrek Bunga Potong Dyah Widyastoety dan Anggraeni Santi Balai Penelitian Tanaman Hias Jalan Raya Ciherang, Pacet-Cianjur 43252 Telp. (0263) 512607, Faks. (0263) 514138, E-mail: [email protected] ABSTRAK. Vanda merupakan salah satu jenis anggrek yang mempunyai nilai ekonomi tingi, tetapi belum dimanfaatkan secara optimal untuk perakitan varietas unggul baru. Jenis anggrek ini sangat penting dan dapat digunakan sebagai tetua persilangan untuk menghasilkan jenis baru yang unik. Sebagian dari jenis anggrek ini dapat digunakan dalam persilangan antar spesies atau antar varietas, sebagian yang lain dapat juga digunakan dalam persilangan antar genus yang berbeda. Dari hasil persilangan menggunakan jenis Vanda, bila diperbanyak secara in vitro dapat menstimulir peningkatan jumlah khromosom, sehingga diperoleh genotip baru yang memiliki karakter-karakter unik yang baru. Kendala pengembangan anggrek di Indonesia antara lain disebabkan terbatasnya ketersediaan bibit unggul, teknologi perbanyakan yang selama ini digunakan masih tradisional, informasi pasar yang tidak jelas dan kurangnya dukungan kebijakan pemerintah. Kata kunci : Anggrek, Vanda, persilangan, bibit, bunga potong ABSTRACT. Dyah Widyastoety dan Anggraeni Santi (2012) The superiority of orchid Vanda group in increasing the variance and quality of cut flower orchids. Vanda is one of the orchid types having high economic value, however is not utilized optimally to produce new superior varieties. This type is very important and can be utilizaed as breeding materials to produce new superior and unique type varieties. Apart from them can be used in interspecies or intervariety crossing, others can be applied in intergeneric crossing. -
THE ORCHIDS Legend: E = Epiphyte; L = Litophyte T = Terrestrial PLANT
Purificacion Orchids Year 2015 RETAIL Quezon Memorial Circle Garden Center http://purificacion.web.fc2.com/ Elliptical Road, Quezon City, Metro Manila, 1100 mobile: +639178510619 PLANT & PRICE LIST - NO MINIMUM VALUE REQUIRED, FREIGHT INCLUDED IN PRICE. All plants are standard in size. For specimen or big sized plants, please inquire about your preference. See Price Schedule below. Refer to our website for pictures: www.purificacionorchids,com BOOKS: 1. PHILIPPINE NATIVE ORCHID SPECIES by Jim Cootes USD $65.00 2. A COLLECTION OF PHILIPPINE HOYAS & THEIR CULTURE by: Fernando B. Aurigue $35.00 International Shows: 1 世界らん展日本大賞 2015年 2月14日(土)~22日(日) 締め切り;2015年1月20日(http://purificacion.web.fc2.com/) 2 Flora Filipina Expo 2015 February 25 to March 9 , 2015, Quezon City, Philippines 3 Dresden International Orchid Show 2015 Dates Pending , Messe Dresden, Dresden, Germany Deadline for Pre-orders: 4 Redland International Orchid Festival 2015 Dates to be announced , Fruit & Spice Park, 24801 SW 187th Avenue Homestead, Florida, USA Deadline for Pre-orders: THE ORCHIDS Legend: E = epiphyte; L = litophyte T = terrestrial PLANT NAME PLANT SIZE PRICE for GRAY - NA (Not Available) USD 1 Accanthephippium mantinianum 4 bulbs T 15.00 2 Acriopsis javanica B/S E 15.00 3 Aerides lawrenciae B/S E 30.00 4 Aerides leeana B/S E 35.00 5 Aerides odorata B/S E 25.00 6 Aerides odorata (Calayan Is.) B/S E 35.00 NET NA 7 Aerides odorata var. alba B/S E 90.00 NET NA 8 Aerides quinquevulnera B/S E 25.00 9 Aerides quinquevulnera var purpurata B/S E 30.00 10 Amesiella minor -
Orchidaceae): the Evolution of Monopodial Leaflessness
MOLECULAR SYSTEMATICS AND ANATOMY OF VANDEAE (ORCHIDACEAE): THE EVOLUTION OF MONOPODIAL LEAFLESSNESS By BARBARA S. CARLSWARD A DISSERTATION PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 2004 Copyright 2004 by Barbara S. Carlsward ACKNOWLEDGMENTS I would like to thank my advisor, William Louis Stern, for his invaluable guidance. He welcomed me into his lab at the beginning of my botany career and has continually nurtured my love of plants and their anatomy. I would also like to thank W. Mark Whitten, who has been a friend and mentor. Without his constant guidance, I would have failed miserably in the molecular laboratory. During the course of my doctoral degree, Norris H. Williams has offered useful counsel, allowed me unlimited access to his laboratory, and given me office space to write my dissertation. From the beginning of my botany career, Walter S. Judd has been instrumental in developing my love for plants and has always given helpful advice in the process of delimiting anatomical data for cladistic analysis. As a committee member, Robert J. Ferl provided insightful advice and guidance. Robert L. Dressler was always willing to help me identify orchids and gave helpful advice on tricky taxonomic issues. James D. Ackerman was also extremely generous with his taxonomic advice, especially regarding Dendrophylax. Gustavo Romero (AMES Herbarium, Harvard University, Massachusetts) provided assistance in finding the taxonomic articles necessary for the nomenclatural transfers of Solenangis. Alec M. Pridgeon has continually been willing to offer guidance and counsel, most notably with the interpretation of the vandaceous anatomy. -
Orchid Judging
ORCHID SOCIETY OF NEW SOUTH WALES Orchid Judging Training Manual ORCHID JUDGING- TRAINING MANUAL FORWORD. This manual of training is designed to be one of many tools used in the training of an orchid judge. It is to be studied in conjunction with the books and articles listed in the Introduction to this manual and has been designed to complement, not replace the Australian Orchid Council Guidelines for Judging Handbook. This Manual combines resources from a number of sources including; Australian Orchid Council, Orchid Society of New South Wales, Queensland Orchid Society, Northern Territory Judging Panel, American Orchid Society. As well as the above ‘formal’ resources, this Manual recognises local panel rules and articles of interest by prominent Orchid personalities. Included in the Biography is a list of internet addresses and other resources which will provide information on orchid registration, orchid species and other related matters. Section 4 of this manual is designed as a series of stand-alone topics which can be addressed or studied in any order. It provides for learning reinforcement with an Assessment of Understanding of the topic. Homework will be included as an integral part of each topic. Some topics include a sub-section titled ‘Comparison with the American Orchid Society …’. This sub-section provides a comparison with the American Orchid Society’s training and judging system and is included because previous students found it beneficial. The American system allocates points to the various components of the flower, eg. shape. (Of course if you do not find this system beneficial, ignore it.) Research into the DNA of orchids is well underway now and may change some of our pre-conceived ideas over time. -
Threatened Plants of the Philippines: a Preliminary Assessment
Threatened plants of the Philippines: a preliminary assessment 1* 2 3 EDWINO S. FERNANDO , LEONARDO L. CO , DANIEL A. LAGUNZAD , 4 5 5 WILLIAM SM. GRUÈZO , JULIE F. BARCELONA , DOMINGO A. MADULID , 6 7 8 AIDA B. LAPIS , GREGORIO I. TEXON , ANTONIO C. MANILA , 9 and PRESCILLANO M. ZAMORA An assessment of the conservation status of the full bryophyte and vascular flora of the Philippines was conducted. The threat categories used follow those previously prescribed and defined in Philippine Republic Act No. 9147, the ‘Wildlife Resources Conservation and Protection Act’ and its Implementing Rules and Regulations which were derived from the 1994 IUCN Categories and Criteria (ver. 2.3), and those in the DENR Administrative Order No. 2004- 15. The resulting list of the threatened plants of the Philippines comprises 694 taxa in the following categories: Critically Endangered 99, Endangered 186, Vulnerable 176, Other Threatened Species 64, and Other Wildlife Species 169. The taxa in the list include 478 angiosperms, 11 gymnosperms, 203 pteridophytes, and two bryophytes (mosses). More than 77 percent of all the taxa listed are endemic to the Philippines. Key Words: endangered plants, Philippines, threatened plants, Wildlife Resources Conservation and Protection Act ------------------------------------------- 1Department of Forest Biological Sciences, College of Forestry and Natural Resources, and Forestry Herbarium, Museum of Natural History, The University of the Philippines – Los Baños, College, 4031 Laguna, Philippines. (*Author for correspondence: -
Haagii: Listed for Botanist and Naturalist, Friedrich Adolph Haage (1796-1866)
haagii: listed for botanist and naturalist, Friedrich Adolph Haage (1796-1866). ex Colombia, cf. Cycnoches haagii Barb.Rodr.1881. habbemae: of Habbema, see habbemense. cf. Phreatia habbemae J.J.Sm.1910. habbemense: from Lake Habbema, central Papua, Indon. cf. Bulbophyllum habbemense P.Royen 1979. Noted as endemic. Habenaria: Willd.1805: ref. habena: a strap; thong + see -are: like, etc. Ref. the long thin lobes of the labellum. ex China, eg. Habenaria oligoschista. habenaria: strap; thong + like. cf. Orchis habenaria L. syn. Habenaria quinqueseta var. macroceratitis. Habenarieae: Habenaria + suffix denotes it’s a tribe. Habenariinae: Habenaria + suffix denotes it’s a subtribe. habenarina: strap + see -are: relative to + see -ina: like, etc. Or, more likely, the raceme was likened to that of a Habenaria. cf. Liparis habenarina. Noted as endemic to Aust. habenarioidea: strap-like + resembling, or see Habenaria + resembles. cf. Orchis habenarioidea. Was listed as such, but may have been an error for Orchis habenarioides (?). syn. Gymnadenia orchidis. habenarioides: Habenaria + resembling. cf. Pterichis habenarioides. x Habenari-orchis: Rolfe. On the syn. list, cf. Habenaria x Orchis. Habenella: Small 1903: ref. habena: thong; strap + suffix: small. Ref. the slender labellum lobes. syn. Habenaria Willd. habenifera: strap + bearing. cf. Lepanthes habenifera. Habenorkis: Thouars 1809: strap + orchid. See expl. for Habenaria. syn. Habenaria. habenula: small strap. cf. Pleurothallis habenula. habit: ref. habitus: condition; disposition (of life). Usually refs. to a peculiarity, feature, or distinction of the species under discussion. habitat: for a plant’s natural home: how and where it grows. Often seen as a heading, in a discussion, or protologue, giving a separate listing and description of the plant’s natural environment and ecology. -
Gardiner, Motes, Emerson, Roberts, Kocyan 1 GARDINER, L.M., MOTES, M., ROBERTS, D.L., KOCYAN, A., EMERSON, B.C; Phylogenetic
Gardiner, Motes, Emerson, Roberts, Kocyan 1 GARDINER, L.M., MOTES, M., ROBERTS, D.L., KOCYAN, A., EMERSON, B.C; Phylogenetic Patterns in the Genus Vanda Phylogenetic Patterns in the Genus Vanda and Related Genera (Orchidaceae) Gardiner, L.M.1,*, Motes, M.2, Roberts, D.L.1,3, Kocyan, A.4, Emerson, B.C.5,6 1Herbarium, Royal Botanic Gardens Kew, Richmond, Surrey, TW9 3AE, UK 225000 162nd Street, Homestead, Florida, 33031, USA 3 Durrell Institute of Conservation and Ecology, School of Anthropology & Conservation, Marlowe Building, University of Kent, Canterbury, Kent, CT2 7NR, UK 4 Biodiversity Research/Systematic Botany, Institute of Biochemistry and Biology, University of Potsdam, Maulbeerallee 2a, D-14469 Potsdam, Germany 5Island Ecology and Evolution Research Group, IPNA-CSIC, C/Astrofísico Francisco Sánchez 3, 38206 La Laguna, Tenerife, Canary Islands, Spain 6School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK *Author for correspondence: ([email protected]) Gardiner, Motes, Emerson, Roberts, Kocyan 2 INTRODUCTION The orchid genus Vanda Jones ex R.Br. comprises approximately 75-80 species and is widely distributed throughout mainly South East Asia; from India and Nepal, through southern China to Korea and Japan, and then down through Indonesia to the northern Australia and the Solomon Islands (Govaerts, 2012). Many species appear to be endemic within a limited geographically range, with a major centre of diversity in the South East Asian archipelagos. Vanda species are epi- and lithophytes exhibiting monopodial growth, stiffly erect, praemorse tipped leaves, and plants range from small through to large, with often brightly coloured, sometimes fragrant, flowers. -
Redalyc.VANDA TRICOLOR LINDL. CONSERVATION in JAVA
Lankesteriana International Journal on Orchidology ISSN: 1409-3871 [email protected] Universidad de Costa Rica Costa Rica GARDINER, LAUREN M. VANDA TRICOLOR LINDL. CONSERVATION IN JAVA, INDONESIA: GENETIC AND GEOGRAPHIC STRUCTURE AND HISTORY Lankesteriana International Journal on Orchidology, vol. 7, núm. 1-2, marzo, 2007, pp. 272-280 Universidad de Costa Rica Cartago, Costa Rica Available in: http://www.redalyc.org/articulo.oa?id=44339813056 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative LANKESTERIANA 7(1-2): 272-280. 2007. VANDA TRICOLOR LINDL. CONSERVATION IN JAVA, INDONESIA: GENETIC AND GEOGRAPHIC STRUCTURE AND HISTORY LAUREN M. GARDINER Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 United Kingdom [email protected] KEY WORDS: Vanda tricolor, haplotype network, nested clade analysis, genetic, geographic, structure Introduction fied from DNA sample 19632 in the RBG Kew DNA bank, collected by the author from Motes’ Orchids in Vanda tricolor Lindl. is widespread in cultivation in Florida, USA, and originally from the Philippines. South East Asia, being relatively easily cultivated in The original collecting localities of the V.tricolor the garden, and is often seen growing floriferously on specimens divided into five clear, non-overlapping garden trees, fence posts and verandas. Whilst the geographic regions so each specimen was assigned to species is widespread in cultivation in its native a geographic region – West Java, Central Java regions of Java and Bali, wild populations are small (including D.I. -
06 June 2014.Pdf
TOWNSVILLE ORCHID SOCIETY INC. Registrar’s Choice June 2014 BULLETIN Full contact details are on our web site http://townsvilleorchidsociety.org.au TOS Inc. Directory of useful information: th Meetings are held 8pm on the 4 Friday of each month except December in the Townsville Orchid Society Inc. hall. Postal Address: Hall Location: PO Box 836 D.C. Joe Kirwan Park AITKENVALE QLD 4814 Charles Street, KIRWAN Hybrids: Rlc. Burdekin Pearl x Rlc. California Girl Patron: Phyllis Merritt gained 82 Points President: Wal Nicholson Ph. 07 4773 4208 C Truscott Secretary: Jean Nicholson Ph. 07 4773 4208 Email: [email protected] Treasurer: Lynne White VP Bulletin: Fred Marnock Mobile: 0438328009 Email: [email protected] Annual Membership Fees: Family $20.00 Pensioner Family $10.00 Single $15.00 Pensioner Single/Junior $7.50 Country Family: $10.00 Details for paying membership fees. BSB:- 064823 Account Number:- 0009 0973 Name of Account:- Townsville Orchid Society Inc. Commonwealth Bank, Aitkenvale Species: B. nodosa gained 79 Points Are you currently un-financial? To remain a member of the Townsville W&C Sewell Orchid Society Inc and to continue to receive the TOS Bulletin you need to pay your annual membership st Fees are due 1 September each year TOS CALENDAR 2014 April 2014 27th June, General Monthly Meeting – 8pm 29th June, - Novice/New Growers’ 4th July, Management Committee Meeting – 7.30pm 2014 Townsville Show Dates: th th th Winter: July - 18 19 20 Spring: September - 19th 20th 21st Specimen Plant: Bulb. spathulatum All show prize money & sales money will be J & A Knowles available for collection at the following - General meeting Friday 25.07.14 and Novice meeting Sunday 27.07.14 June Judges Masters A.