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(12) Patent Application Publication (10) Pub. No.: US 2009/0246343 A1 Wu Et Al

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(12) Patent Application Publication (10) Pub. No.: US 2009/0246343 A1 Wu Et Al US 20090246343A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2009/0246343 A1 Wu et al. (43) Pub. Date: Oct. 1, 2009 (54) STABLE NATURAL COLOR PROCESS, Publication Classification PRODUCTS AND USE THEREOF (51) Int. Cl. (75) Inventors: Shaowen Wu, Cincinnati, OH (US); A2.3L I/275 (2006.01) Chad Ford, Walton, KY (US); C09B 6L/00 (2006.01) Gregory Horn, Wyoming, OH (US) D06P 5/00 (2006.01) Correspondence Address: (52) U.S. Cl. .................................. 426/540: 8/646; 8/400 FROST BROWN TODD, LLC 2200 PNCCENTER, 201 E. FIFTH STREET CINCINNATI, OH 45202 (US) (57) ABSTRACT (73) Assignee: Wild Flavors, Inc., Erlanger, KY A method of preparing colored products from edible materi (US) als comprises processing Genipa americana fruit juice, which contains genipin, genipin derivatives, or pre-genipin (21) Appl. No.: 12/399.223 compounds, with otherediblejuices or extracts which contain nitrogenous compounds such as amino acids, polypeptides, (22) Filed: Mar. 6, 2009 or proteins. The generated natural colored products have excellent stability and can be used in a broad range of appli Related U.S. Application Data cations including beverages, foodstuffs, drugs, dietary (60) Provisional application No. 61/040,208, filed on Mar. Supplements, cosmetics, personal care stuffs, and animal 28, 2008. feeds. Huito Juice Process Example 3 Fresh selected ratio of huito to Watermelon Watermelon Mill/Pressing (Juice/Puree) Low Temp. Heating at 35-40C for M1 Hour Clarification (e.g. Filtration/Screening? Centrifugation) Solids Color Checks: Hunter Lab Colorimeter High Temp. Heating Color Check Dilution at 75-80C for 1-2 Hours To C.V.O.5 D H2O Concentration Color intensity Check UVFWS Spectrophotemeter (Vacuum Distillation) Dilution 0.1g/100 mL DI H20 Pasteurization (High Temp Short Time) Standardization - Final Oc Patent Application Publication Oct. 1, 2009 US 2009/0246343 A1 Figure 1 - Huito Juice Process Example 3 Fresh selected ratio of huito to watermelon Watermelon Mill/Pressing (Juice/Puree) Low Temp. Heating at 35-40C for a 1 Hour Clarification (e.g. Filtration/Screening/ Centrifugation) Color Checks: High Temp. Heating Color Check Hunter Lab Colorimeter at 75-80C for 1-2 Hours ToDilution C.W. O.5 D H2O Concentration colorColor intensity Checkcheck UVWIS Spectrophotemeter (Vacuum Distillation) Dilution 0.1 g/100 mL DI H20 Pasteurization (High Temp Short Time) Standardization - Final Oc US 2009/0246343 A1 Oct. 1, 2009 STABLE NATURAL COLOR PROCESS, The principal biochemical compounds of Genipa americana PRODUCTS AND USE THEREOF include: calcium, phosphorous, vitamin C, and caffeine, caterine, genipic acid, genipin, genipin gentiobioside, geni pinic acid, geniposide, geniposidic acid, gardenoside, 0001. This application is related to and claims priority genamesides A-D. gardendiol, deacetyl asperulosidic acid from U.S. Provisional Application Ser. No. 61/040,208, Wuet methyl ester, Shanzhiside, glycerides, hydantoin, mannitol, al, filed Mar. 28, 2008, incorporated herein by reference. methyl esters, tannic and tartaric acid, and tannins. 0007. The present disclosure relates to the preparation of TECHNICAL FIELD stable colorants by mixing and co-processing fruit juice, par ticularly from Genipa americana, with other edible juices or 0002 This disclosure relates to natural stable color prod extracts from fruit, vegetable, plant materials, grain, legume, ucts which can be used in a broad range of applications nuts, seeds, animal materials including milk and egg, micro including foodstuffs, drugs, nutritional Supplements, per bial, and algal materials, which contain amino acids, or Sonal care stuffs, cosmetics, and animal feeds, and a process polypeptides, or proteins. The product colors are not those for preparing these colored food and edible products. Specifi expected from simple pigment blending, and the color prod cally, the disclosure relates to the stable color products that ucts have great stability to acidity and heating. The co-pro are generated by processing Genipa americana fruit juice cessing of Genipa americana fruit with other selected fruit(s) which contains genipin, genipin derivatives, or pre-genipin (defined broadly as above to include also grains and animal compounds, together with other edible juices or extracts materials) to obtain the composite juice is simple and effec which contain amino acids, polypeptides, proteins, and com tive. The products can be used in a broad range of applica pounds with one or more primary amine groups. tions, such as foodstuffs, drugs, nutritional Supplements, per Sonal care stuffs, cosmetics, and animal feed. BACKGROUND 0003. Today, a decreasing number of people are willing to SUMMARY OF THE INVENTION eat or drink foodstuffs and use cosmetic products which are 0008. The present disclosure provides a method of pro colored synthetically, resulting in a steady growth of the ducing natural stable color products wherein Genipa ameri market for natural colorants. Thus, a natural color, especially cana fruits are co-processed with other edible juices or a natural blue colorant that is stable at low pH and to tem extracts from fruits, vegetables, plant materials, grains, peratures used in processing, would be of significant world legumes, nuts, seeds, animal materials including milk and wide commercial interest. The only natural blue colors com eggs, microbial, and algal materials that contain amino acids, mercially feasible today are those derived from gardenia polypeptides, and/or proteins. fruits (Gardenia jasminoides Ellis). However gardenia blue is 0009. The present disclosure further provides natural not currently available in US and Europe markets, it is only color products that have desirable organoleptic properties and available in Some Asian markets as a safe color product for excellent compatibility with foods and food components, are food/drug applications. intrinsically acceptable to consumers due to the nature of 0004 Gardenia fruit contains a large amount of iridoids their raw materials and have certain nutritional values, and Such as geniposide, gardenoside, genipin-1-b-gentiobioside, which also have very good stability over abroad pH range and geniposidic acid and genipin (Endo, T. and Taguchi, H. good resistance in use to heat and light. Therefore, the prod Chem. Pharm. Bull. 1973). Among them genipin is a key ucts are Suitable for use in various applications, such as food compound contributing to the gardenia blue whenever it stuffs, drugs, nutritional Supplements, personal care stuffs, reacts with amino acids (U.S. Pat. No. 4,878.921). Currently cosmetic, and animal feed. gardenia blue is made as a chemical reaction product using geniposide extracted from Gardenia juice, purified genipin, BRIEF DESCRIPTION OF THE DRAWINGS or genipin derivatives, with isolated amino acids. In contrast, 0010 FIG. 1 is a flow chart representation of the process the process of the present invention utilizes whole fruit, puree or juice of genipin-containing plants to provide a natural color described in Example 3. juice or concentrate. DETAILED DESCRIPTION OF THE INVENTION 0005 Genipin and other iridoid compounds, such as geni pic acid, genipin gentiobioside, geniposide and geniposidic 0011. The present disclosure provides a method of pro acid are found also in the fruits of Genipa americana, also ducing natural stable color products by using Genipa ameri known as Genipap, or Huito, a wild plant of tropical Latin cana fruit juice with the various added edible materials. Natu American. The mature fruits of Genipa americana have been ral stable blue color is believed to be obtained when genipin commonly used by local people to make cooling drinks, jel and possibly genipin gentiobioside, which naturally exist in lies, sherbet, ice cream, Sweet preserves, syrup, a soft drink— Genipa americana fruit, react with amino acids, polypep genipapada, wine, a potent liqueur, and tanning extract. tides, or proteins, and other compounds with primary amine Green or unripe fruits are used as a color source to paint faces groups, in the various edible materials. All known and prac and bodies for adornment, to repel insects, and to dye cloth ticed juice extraction techniques and operations, and process ing, pottery, hammocks, utensils and basket materials a blu ing technologies associated with non-juice components ish-purple. The fruit and juices also have medicinal properties claimed, are considered Suitable to prepare the color juices of and syrups are used as cold and cough medicine. Flowers and this invention and are incorporated by reference herein. bark of Genipa americana also have medicinal properties. 0006 Genipa americana is also a natural source of iron, Starting Materials riboflavin and anti-bacterial Substances, apart from the car 0012. The starting materials used in the process of the bohydrates, Sugar, proteins, ash and malic acid in its fruits. present invention are mature fruits of Genipa americana L.; US 2009/0246343 A1 Oct. 1, 2009 also known by numerous informal names: genipap, huito, (Form FDA 2438g (10/91) (Root and Tuber, Bulb, Leaf & jaguar, bilito, cafecillo denta, caruto, caruto rebalsero, confi Stem, Brassica, Legume, Fruiting and Curcubit Vegetables) ture de singe, danipa, genipa, génipa, genipayer bitu, guaitil, Such as bean sprouts, green cabbage, celery, onions, Sweet guaricha, guayatil colorado, huitol, huitoc, huitu, irayol, onions, asparagus, any leaf vegetables, green beans, peas, jagua blanca, jagua amarilla, jagua colorado, jeipapeiro, juni cauliflower,
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