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Correlation of Fine Needle Aspiration Cytology, Smear and Culture In Original Article Objective The study was carried out to assess the frequency of pain and Correlationwithdrawal movement ofs Fineafter Needle Aspiration Cytology, Smear injection of rocuronium and andeffects ofCulture pre-treatment within Tuberculous Lymphadenitis: A Retrospectivelignocaine. Study Design Shazia Aslam, Sadia Hameed, Arif Hussain, Tariq Gulzar Bhatti, Mrs Nida Attique It was a double blind study. Place and Duration of Study AbstractThis study was of six months Tuberculousduration and lymphadenitis was carried isout the commonest study at, Meezan lab Faisalabad. Material and formfrom ofMarch extrapulmonary 2004 to September tuberculosis. Fine Methods: 100 patients of lymphadenitis which needle2004 aspirationat Combined cytology Milita (FNAC)ry is a simple were diagnosed as cases of granulomatous outHospital-patient Kharian. diagnostic procedure used for the inflammation on FNAC were included in this diagnosisPatients andof tuberculousMethods lymphadenitis. Over study. These cases were reported on cytology theOne last hundred two anddecades, twenty fine needle aspiration by using the Giemsa stain, H& E stain, Gram & unpremedicated patients with cytology has emerged as a simple outpatient Ziehl Neelsen stain. After the smear preparation ASA grade I and II, aged the part of left over aspirated material was diagnosticbetween 18 procedure-60 years and for of boththe evaluation of tuberculoussexes were enrolledlymphadeni in thetis. study. This has replaced inoculated on LJ medium and were reported on excisionPatients biopsywere randomly of lymph divided node. In this study, the basis of morphological features by FNACinto two wasgroups complementedof 60 patients with smear concerned microbiologist. Results: Out of these examinationeach. After inductionand culture of for AFB. It was 100 selected reported cases of tuberculous observedanaesthesia that with out thiopentone, of the 100 reported cases of lymphadenitis culture revealed growth of tuberculosispatients in group on FNAC A, received direct 3 smear positivity Mycobacterium on 27 of them. While direct onml ZN of lignocaine staining plainwas while 3/1 00(3%). After the microscopic examination of the ZN stained inoculationthose inArticle of residual aspirated material on LJ smears from these aspirates revealed the medium the culture yield was 27/100(27%). presence of AFB in only 3 out of 100 cases. Thus FNAC had greater diagnostic efficacy, Key Words: Mycobacterium tuberculosis, proved to be a rapid, less time consuming & lymphadenitis, fine needle aspiration cytology non-invasive screening test for evaluation of & ZN staining (Ziehl Neelsen), Lowenstein tuberculous lymphadenitis. Settings and Jensen medium. Design: A retrospective laboratory based INTRODUCTION Tuberculosis is thought to be one of the oldest almost as old as mankind. Evidence of its human diseases. The history of tuberculosis is existence was seen in Egyptian mummies and statuaries in the form of Pott’s disease of spine.1 Corresponding Author: Since the identification of Mycobacterium Dr. Sadia Hameed tuberculosis as etiologic agent for tuberculosis by Professor of Pathology Robert Koch in 1882, there have been great University Medical and Dental College Faisalabad advances in our understanding of many of the Tel. +92333-6525500 crucial aspects in its pathogenesis.2 While E-mail: [email protected] A.P.M.C Vol: 7 No. 2 July-December 2013 114 pulmonary tuberculosis is no doubt common, asymptomatic. Some patients may manifest tuberculous lymphadenitis is the most common systemic symptoms such as fever, weight loss, form of extrapulmonary tuberculosis.3 fatigue, and occasional night sweats. Tuberculosis waxed and waned in Europe during Fine needle aspiration cytology is now established 18th and 19th centuries. During industrial as an alternative, easy and rapid method of tissue revolution it claimed millions of lives in Europe diagnosis. It also has a high degree of patient’s and so was called ‘The White Plague’. According acceptance as FNAC avoids physical and to World Health Organization tuberculosis still psychological trauma occasionally encountered after kills three million people every year in biopsy due to anaesthesia, surgical procedure and underdeveloped countries.4 hospitalization. It is very safe, cost effective and Primarily considered to be a pulmonary disease, conclusive.9, 10 Tuberculosis can affect almost any organ or Mycobacteria are slow growing and hence culture systems of human body. The term extra is not done routinely in all laboratories. Few pulmonary tuberculosis has been used to describe studies have tried to correlate the cytological isolated occurrence of tuberculosis at various finding with microbiological results for the body sites other than the lung. The most common presence of acid-fast bacilli in smears and culture sites of extra pulmonary tuberculosis consist of for mycobacteria.11,12 The present study was gastrointestinal tract (GIT), Lymph nodes, undertaken to correlate the cytomorphologic genitourinary system, bone, joint and central features of lymph node aspirates of tuberculous nervous system involvement followed by lymphadenitis to bacteriologic studies that peritoneal and other organ involvement.5 included both smear and culture for acid fast Tuberculous lymphadenitis in the cervical region bacilli (AFB). is termed as scrofula. The microbiological cause of scrofula was first appreciated by Bollinger, AIMS & OBJECTIVES May and Demme in the mid to late 19th century The current study was conducted with following when they noted that Mycobacterium bovis from 6 objectives. cows was the cause of this ailment. The 1. To determine efficacy of FNAC in detecting conventional methods of diagnosis for tuberculous lymphadenitis. tuberculosis like examination of sputum for Acid 2. To evaluate the role of ZN staining and fast bacilli and chest x-ray are fairly accurate in culture of aspirated material in detecting detecting the active pulmonary component of the tuberculous lymphadenitis. disease. However they are not useful for detecting extra pulmonary components. The diagnosis of MATERIALS & METHODS lymph node tuberculosis usually rests on a lymph node biopsy with or without bacteriological The cases of this study came from those patients studies.7 with lymphadenopathy referred to Meezan lab for The unusual features of TB lymphadenitis are its routine aspiration diagnosis from November 2011 gender and age distribution, as it is more common to April 2013. All of the 100 aspirates diagnosed in females and in the younger age groups, in as tuberculous lymphadenitis were subjected to contrast to pulmonary tuberculosis which is more mycobacterial culture & ZN (Ziehl Neelsen) stain common in males and in the older age group. It for AFB on directly prepared smears. has a peak age of onset at 20 to 40 years 08. Fine needle aspiration cytology was performed Patients usually present with slowly enlarging aseptically by using 24 G needle and 5ml or 10ml lymph nodes which may otherwise be BD syringe. The smears were stained by Giemsa stain, H& E stain and by the Ziehl Neelsen A.P.M.C Vol: 7 No. 2 July-December 2013 115 technique for AFB. The contents of the needle smear positivity was 30/100 (30%) (Figure-2). were also inoculated on Lowenstein Jensen Among these majority cases 17/100(14%) were medium. Morphology of the growth appearing belonging to chronic granulomatous inflammation after 2-3 weeks of incubation was checked by with superadded pyogenic infection (category 3) AFB smear. The negative cultures were incubated (Figure 3, 4), 10/100 cases were of the chronic for 6-8 weeks before discarding them. The criteria granulomatous lymphadenitis group (category2) used for making the diagnosis of tuberculosis on (Figure 5-7) and 3/100 cases were belonging to FNA aspirates were presence of granulomas class of necrotic or caseous material (category 1). comprising of epithelioid cells with or without giant cells and presence of necrotic material with Table-1 or without epithelioid cells and /or ZN smear Frequency of granulomatous inflammation / positivity for Acid Fast Bacilli (AFB) and/or Tuberculosis at various sites positive culture for Mycobacteria. Cervical lymph nodes 52% RESULTS Supraclavicular lymph nodes 14% The study comprises of 100 cytologically Submandibular lymph nodes 14% diagnosed cases of tuberculous lymphadenitis at Axillary lymph nodes 09% Meezan lab Faisalabad. The age of the patients ranged from 3.5 to 57 years, the majority being in Preauricular lymph nodes 03% the age group of 10-30 (70%). The male to female Submental lymph nodes 01% ratio was 38:62. The majority of aspirations were Inguinal lymph nodes 01% from cervical lymph nodes (52%) followed by Gluteal region swelling 02% supraclavicular (14%) and submandibular lymph Left shoulder swelling 01% nodes (14%) (Table no 1). The nature of the material aspirated was variable. Swelling in front of neck 01% Thin liquefied pus like material, frankly purulent Discharging sinus cervical region 01% material, typical caseous material or solid particles Breast swelling 01% were noted on gross examination. Based on the cytomorphology smears were categorized into Table-2 three types: Correlation of cytomorphologic features with smear AFB positivity in cytologically proved 1. Necrotic or caseous material only lymphadenitis cases 2. Necrotic material with typical epithelioid granulomas; or Cytologic Number Smear Culture 3. Epithelioid granulomas or cells in background category positivity (%) positivity (%) of superadded pyogenic
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