The Abstracts
Total Page：16
File Type：pdf, Size：1020Kb
THE ABSTRACTS Committee & International Boards Executive President of the Congress B Boris Vargaftig Brazil President of the Brazilian Society of Immunology Sérgio Costa Oliveira Brazil Honorary President of the Congress Sérgio Henrique Ferreira Brazil Executive Committee Consulting Scientific Committee Manoel Barral Neto Brazil Fernando Queiroz Cunha - Alberto Mantovani Italy Marc Peters Golden USA Treasurer Brazil Alexandre Salgado Basso Brazil Marcellus Souza Brazil João Santana da Silva Brazil Amanda Proudfoot Switzerland Marcelo Torres Bozza Brazil José Carlos Alves Filho Brazil Ana Campa Brazil Marco Aurélio Martins Brazil Mauro M. Teixeira Brazil Andrew Luster USA Marco Cassatella Italy Momtchillo Russo Brazil Antônio L . Teixeira Jr Brazil Mauro Martins Teixeira Brazil Niels O. S. Camara - Executive B. Boris Vargaftig Brazil Mauro Perretti England Secretary Brazil Bart Lambrecht Belgium Michael Parnham Germany Patrícia Torres Bozza Brazil Bernard Ryffel France Michel Chignard France Scientific Committee Cristovam Picanco Diniz Brazil Micheline Lagranderie France Mauro Martins Teixeira (Chair) Danielle G. Souza Brazil Miguel Soares Portugal Brazil Dario Zamboni Brazil Moises Bauer Brazil Fernando Queiroz Cunha Brazil Doulgas Golenbock USA Momtchillo Russo Brazil José Carlos Alves Filho Brazil Eddy Liew Scotland Niels O. S. Camara Brazil Kouji Matsushima Japan Edgar Carvalho Brazil Nikita Lomakin Russia Michel Chignard France Edson Antunes Brazil Patrícia Torres Bozza Brazil Momtchillo Russo Brazil Eugen Faist Germany Paul Foster USA Niels O. S. Camara Brazil Fernando Queiroz Cunha Brazil Paul Kubes Canada Patricia Torres Bozza Brazil Flávia do Nascimento Brazil Rafael Radi Uruguay Thiago M. Cunha Brazil Francisco Airton Rocha Brazil Reinaldo Salomão Brazil Local Committee Francisco R. M. Laurindo Brazil Renato Monteiro France Selma Maria Bezerra Jerônimo Gerard J Graham Scotland Renato S. B. Cordeiro Brazil Brazil Graham Wallace Scotland Ricardo Gazzinelli Brazil Janeusa Trindade de Souto Brazil Guy Zimmerman USA Roger Chammas Brazil Janaina Cristiana de Oliveira Hamida Hamina Belgium Ronaldo Ribeiro Brazil Crispim Brazil Ian Mclnnes Scotland Ruslan Medzhitov USA Abstracts Committee Jamil Assreuy Brazil Sandra Farsky Brazil Alexandre Basso Brazil João Batista Calixto Brazil Sérgio Costa Oliveira Brazil Alexandre Keller Brazil João Santana da Silva Brazil Sérgio Henrique Ferreira Brazil André Luiz Barbosa Báfica Brazil John Somerville USA Sergio Lira USA João Trindade Marques Brazil John Wallace Canada Sonia Jancar Brazil Marcos Augusto Grigolin Grisotto Brazil Jorge Kalil Brazil Steve Kunkel USA José Carlos Alves Filho Brazil Tereza Cristina Barja Fidalgo Brazil Julio Scharfstein Brazil Thiago M Cunha Brazil Kouji Matsushima Japan Timothy J Williams England Lisa Marshall USA Vincent Lagente France Lucia H. Faccioli Brazil Wilson Savino Brazil Luiza Karla Arruda Brazil Wim Van Den Berg Netherlands Luke O’Neil Ireland Wothan Tavares de Lima Brazil ALLERGY (AL) 2 ACTIVATION OF PROTEASE-ACTIVATED RECEPTOR 2 PROMOTES ALLERGIC SENSITIZATION TO BACTERIAL ENZYME ESTHER FLORSHEIM1; IVAN BRAGATTO2; ELIANE GOMES3; RUSLAN MEDZHITOV4; MOMTCHILO RUSSO5. 1,3,5.UNIVERSIDADE DE SAO PAULO, SAO PAULO - SP - BRASIL; 2.UNIVERSITÉ DE STRASBOURG, STRASBOURG - FRANÇA; 4.YALE UNIVERSITY, NEW HAVEN - ESTADOS UNIDOS. Proteases used in industrial, pharmaceutical and commercial applications are of increasing prevalence and importance. However, this has resulted in the sensitization of numerous individuals, resulting in the widespread occurrence of allergic reactions to these proteins, including occupational asthma (OA), which is also the most common form of pulmonary disease related to work. For example, many workers from detergent industries developed OA when exposed to the serine protease subtilisin Carlsberg (SC) during the 60s. Today, hypersensitivity to proteins in working areas continues to be a serious public health problem. Since the mechanisms underlying allergic sensitization to proteases are still unknown, we aimed to investigate the role of innate immune control on SC-induced Th2 inflammation. Here we establish an experimental model of OA and suggest the allergic response triggered by SC is dependent on its serine protease activity. We also show that protease-activated receptor 2 (PAR-2) plays a key role in the development of SC-induced type 2 inflammation, as assessed by eosinophil migration to the airways, mucus production by pulmonary goblet cells, increase in total IgE and specific IgG1, IL-4 production by lungT CD4+ cells and also secretion of IL-5 and IL-13 by both classical T and innate lymphoid cells type 2. Notably, human epithelial cells (cell line H292) rapidly respond to SC activity by secreting early pro-Th2 cytokines, such as IL-1a, TSLP and IL-33. Therefore, we propose a mechanism for SC allergenic activity based on epithelial sensing of serine protease through PAR-2 and initiation of a classical Th2 response. Financial support: FAPESP, CNPq 3 ALLERGIC LUNG INFLAMMATION, BUT NOT IGE PRODUCTION, INDUCED BY ALUM-FREE SENSITIZATION TO BLOMIA TROPICALIS IS DEPENDENT ON MYD88 ADAPTOR MOLECULE NICOLE HUNE YOKOYAMA; BIANCA BALBINO; KARINA MENDES MELCHUNA; BETHANEA CREMA PEGHINI; LUCIANA MIROTTI; ELIANE GOMES; MOMTCHILO RUSSO. UNIVERSITY OF SAO PAULO (USP), SAO PAULO - SP - BRASIL. Introduction: Blomia tropicalis (Bt) is an important agent of mite-induced asthma in tropical regions. Previously, we have developed an asthma-like model using Bt extract where the animals were sensitized subcutaneously (s.c.) to Bt extracts adsorbed onto alum and challenged with Bt. In this model, allergic sensitization is independent of the adaptor molecule MyD88 of Toll-IL1 receptor. Objectives: The aim of this work was to investigate whether sensitization with Bt in absence of alum induces allergic lung inflammation and to determine the role MyD88 molecule in this process. Methodology: C57BL/6 WT or MyD88KO mice were sensitized i.n. or s.c. with Bt (50ug) on days -2, -1 and 0 and challenged i.n. with Bt (10ug) on days 7, 14 and 21. Airway inflammation was determined by total and differential cell counts in bronchoalveolar lavage (BAL) fluid and by flow cytometry. The levels of cytokines and total serum IgE were measured by ELISA. Results: In WT mice, i.n. or s.c. sensitization induced significant increase in the total number of inflammatory cells, composed mainly of neutrophils and eosinophils, and increased the production of total IgE when compared with naïve mice (3,0 and 4,6ug/mL, respectively, vs. 0,4μg/mL). In contrast, MyD88-deficient mice almost did not develop airway inflammation. However, the production of IgE by Bt-sensitized and -challenged mice was significantly increased (2,8 and 2,9μg/mL, respectively) when compared with naïve MyD88 mice (0,7μg/mL), reaching similar levels to those obtained in WT mice. Conclusions: Our results indicate that sensitization with alum in not required for the development of airway allergic inflammation. The recruitment of inflammatory cells to the lung is dependent on signaling via Toll/IL-1 receptor domain adaptor protein MyD88. Finally, the Bt induced production of IgE is MyD88 independent. Financial support: FAPESP (2009/07208-0); CNPq; CAPES. 4 ALTERNATIVE METHODS: USE OF A HUMAN ACUTE MONOCYTIC LEUKEMIA CELL LINE TO PREDICT ALLERGENIC POTENCIAL OF CHEMICAL AGENTS CAROLINA BELLINI PARISE1; VANESSA MOURA SÁ-ROCHA2; JANE ZVEITER MORAES3. 1,3.UNIFESP, SÃO PAULO - SP - BRASIL; 2.NATURA LTD, CAJAMAR - SP - BRASIL. Introduction: Chemical exposure can lead to contact dermatitis, affecting 15-20% of general population. New chemical products are emerging each day and modification in the regulation for cosmetics in Europe prohibiting animal tests is a reality since March 2013. The European Union-Funded Program on the Development of Novel Testing Strategies for In Vitro Assessment of Allergens has already shown that myelomonocytic cell lines are activated by the presence of some chemicals, working as antigen presenting cells in in vitro assays. Allergens induce an exaggerated immune response and irritants, although cause similar symptoms, are different from allergens, in that way they do not trigger the allergic response from the immune system cells. The goal of this work is develop and pattern an in vitro methodology capable to distinguish sensitizers from irritants chemicals. Methodology and Results: In this work, a human acute leukemia cell line THP-1 has been incubated with sensitizers (11) and irritant (8) chemicals described in literature. For each chemical, the required concentration to 50% of cytotoxic effect (EC50) was determined, as well as the concentration of cell viability (CV) 75% that allowed the detection of allergenic indicators. The CV75 was employed to analyze the CD86 expression on cell surface and IL-8 secretion in the culture supernatants after 24 h-incubation of THP-1 cells with each chemical. Exposure to irritating chemicals did not result in detectable CD86 expression and did not induce IL-8 secretion or Il-8 gene expression. In contrast, when THP-1 cells were incubated with chemical sensitizers IL-8 secretion and/or CD86 expression were induced. Conclusion: In our conditions, the analysis of CD86 expression combined with those of IL-8 release, after chemical exposure, allowed differentiating sensitizers from irritant chemicals. Grant support: FAPESP, CAPES and Natura LTD. 5 ANNEXIN A1 NEGATIVELY REGULATES THE RELEASE OF HISTAMINE AND EICOSANOIDS IN THE HUMAN AND MURINE MAST CELLS.