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2.4.2. Demonstration of Joystick Cutting

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2.4.2. Demonstration of Joystick Cutting UC San Diego UC San Diego Electronic Theses and Dissertations Title Development of a dual joystick-controlled optical trapping and cutting system for optical micro-manipulation of cells Permalink https://escholarship.org/uc/item/3t97p293 Author Harsono, Marcellinus Stevie Publication Date 2011 Peer reviewed|Thesis/dissertation eScholarship.org Powered by the California Digital Library University of California UNIVERSITY OF CALIFORNIA, SAN DIEGO Development of a Dual Joystick-Controlled Optical Trapping and Cutting System for Optical Micro-manipulation of Cells A thesis submitted in partial satisfaction of the requirements for the degree Master of Science in Bioengineering by Marcellinus Stevie Harsono Committee in charge: Professor Michael W. Berns, Chair Professor Michael Heller Professor Yu-Hwa Lo Professor Jeffrey H. Omens 2011 Copyright Marcellinus Stevie Harsono, 2011 All rights reserved. The Thesis of Marcellinus Stevie Harsono is approved and it is acceptable in quality and form for publication on microfilm and electronically: ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ Chair University of California, San Diego 2011 iii For my mother, Maria, my father, Adrianus, and my sister, Stella and her fiancé, Glenn, as well as for all of my friends. iv TABLE OF CONTENTS Signature Page ......................................................................................................................................... iii Dedication. ............................................................................................................................................... iv TABLE OF CONTENTS .......................................................................................................................... v LIST OF TABLES ................................................................................................................................ viii LIST OF FIGURES .................................................................................................................................. ix ABBREVIATIONS AND SYMBOLS................................................................................................... xiv ACKNOWLEDGMENTS ...................................................................................................................... xvi VITA ..................................................................................................................................................... xvii ABSTRACT OF THE THESIS ........................................................................................................... xviii I. Introduction and Background ........................................................................................................... 1 1.1. Optical Tweezers Theory ............................................................................................................... 1 1.2. Optical Scissors Theory ................................................................................................................. 4 1.3. Existing System Setup.................................................................................................................... 6 1.3.1. Hardware Setup ...................................................................................................................... 7 1.3.2. Optical Setup .......................................................................................................................... 8 1.3.3. Software: Robolase IV .......................................................................................................... 10 1.4 Objectives...................................................................................................................................... 11 References ........................................................................................................................................... 13 II. Development of the Joystick User Interface ................................................................................... 14 2.1. Needs Assessment and Problem Formulation .............................................................................. 14 2.2. Design Alternatives ...................................................................................................................... 14 2.2.1. Design Goals and Specifications .......................................................................................... 14 2.2.2. Design Alternative #1: Simple Two-Axis Joysticks ............................................................. 17 2.2.3. Design Alternative #2: Three-Axis Joysticks with Buttons .................................................. 18 2.2.4. Design Alternative #3: Touchscreen Interface ...................................................................... 18 2.2.5. Mouse ―Point-and-Click‖ Interface ...................................................................................... 20 2.2.6. Decision Matrix .................................................................................................................... 22 2.3. Proposed Solution ........................................................................................................................ 26 2.3.1. Hardware: Custom Industrial Joysticks ................................................................................ 26 2.3.2. Earlier Control Schemes ....................................................................................................... 27 2.3.2.1. Positional Control .......................................................................................................... 28 2.3.2.2. Velocity Control ............................................................................................................ 30 2.3.3. Current Control Scheme: Onboard/Simultaneous Control .................................................... 33 2.3.3.1. Onboard Control Scheme .............................................................................................. 33 2.3.3.2. Hardware Setup ............................................................................................................. 34 2.3.3.3. Control by User ............................................................................................................. 41 v 2.3.3.4. Software ........................................................................................................................ 41 2.3.3.5. Limitations..................................................................................................................... 44 2.3.3.6. Simultaneous Program................................................................................................... 45 2.4. Preliminary Demonstrations ......................................................................................................... 48 2.4.1. Demonstration of Joystick Trapping ..................................................................................... 48 2.4.2. Demonstration of Joystick Cutting ....................................................................................... 50 2.4.3. Combined Demonstration of Joystick Trapping and CutROI ............................................... 51 References ........................................................................................................................................... 54 III. Experimental Studies .................................................................................................................. 55 3.1. Introduction and Hypothesis ........................................................................................................ 55 3.2. Methods ........................................................................................................................................ 57 3.2.1. Indian Muntjac Isolated Chromosomes ................................................................................ 57 3.2.2. PtK2 and Indian Muntjac Control ......................................................................................... 58 3.2.3. PtK2 and Indian Muntjac with Nocodazole .......................................................................... 59 3.2.4. PtK2 GFP-Tubulin Using Laser Ablation with Nocodazole and BrdU ................................ 59 3.2.5. New System Design and Stronger Trap ................................................................................ 60 3.2.6. Expected Data and Statistics (Fisher Exact Test) ................................................................. 62 3.3. Imaging Data and Results ............................................................................................................ 63 3.4. Summary of Results ..................................................................................................................... 69 References ........................................................................................................................................... 71 IV. Discussion .................................................................................................................................. 72 4.1. Discussion: Development of the Joystick User Interface ............................................................. 72 4.2. Discussion: Experimental Studies ...............................................................................................
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