Tail Flick Meter for Evaluating Thermalanalgesia Analgesia

Tail Flick Meter Tail Flick Meter This system features radiant heat applied on the animal’s tail; when the animal feels discomfort, it reacts by a sudden tail’s movement (tail flick) which automatically stops the stimulation and the timer for the measurement of the animal reaction time (period from the beginning of the stimulation until detection of the animal’s response). This test has proved particularly sensitive for studying the analgesic properties of pharmacological substances. It can also be used to evaluate basal thermal pain sensitivity or to study putative genetic differences among control animals. The LE7106 Tail-flick Meter consists of a stimulation unit (containing the halogen lamp for the heat stimulus) and an electronic control unit. The system can be used for rats and mice of different sizes. The animals are Key Features placed with its tail protruding within a restraining tube on the platform of the • Optimal detection due to perfect alignment of stimulus unit. The animal’s tail is positioned on a slot of adjustable width heat stimulus and photo beam trigger equipped with a groove that guarantees a correct placement. A remote foot- • Photo beams with adjustable sensitivity switch controls the test start/stop allowing rapid hands-free experiments. • A light beam shows the point on which the heat A photo beam with adjustable sensitivity detects the tail flick and the source will focus latency is automatically presented on a digital display on the control unit. Measurements of reaction time are given with a 0.1 precision. A cut-off • Manual and remote timer time can be set to avoid tissue damage (by default: 20 s). The groove • Groove for correct tail placement system for the tail and the adjustment of response sensitivity ensure • Automatic cut-off optimum repeatability and reliability of results. • Computer interface SeDacom software supplied with the unit can be used to automatically record the results on a PC through a RS-232 port. Parameters Measured • Time latency responses to thermal stimulus Specifications Components Included Control Unit 350 (W) x 350 (D) x 130 (H) Dimensions • Control Unit with RS-232 port to PC Stimulation Unit 400 (W) x 140 (D) x 155 (H) • Stimulation unit Dimensions • Mouse tail adapter Power Supply 110V/220V, 50/60Hz • Holder for rat or mouse Material Composition Methacrylate, halogen lamp Computer Requirements PC (Windows 95, 98, ME, NT, 2000 and XP) • Footswitch Maximum Number 1 per computer (multiple set-ups • SeDaCom software of Stations also available under request) • Instruction manual Certifications CE compliant • Calibration certificate • Cables and connectors Model Product Order # Price $ • Set of spare fuses LE7106 Tail Flick Analgesia Meter BH1 76-0293 6,493.00 Including SeDaCom Software • 2 year warranty Options Options LE7000 Thermal Printer BH1 76-0114 1,341.00 • LE7000 Thermal printer LE7106T Tail-Temperature Recorder BH1 76-0294 1,644.00 • LE7106T Tail-Temperature recorder Citations Andriambeloson E et al. (2006) Interleukin-6 attenuates the development of experimental diabetes-related neuropathy. Neuropathology. 26(1): 32-42. (rat, France) Cendán CM et al. (2005) Antinociceptive effects of haloperidol and its metabolites in the formalin test in mice. Psychopharmacol. 182(4): 485-493. (mouse. Spain) Grillet N et al. (2005) Generation and characterization of Rgs4 mutant mice. Mol. Cell. Biol. 25(10): 4221-4228. (mouse, France) Moncada A et al. (2005) Inhibitors of serine/threonine protein phosphatases antagonize the antinociception induced by agonists of alpha 2 adrenoceptors and GABAB but not kappa-opioid receptors in the tail flick test in mice. Pain 114(1-2): 212-220. (mouse, Spain) Tail Flick Meter and Hot Plate Analgesiaanalgesia Meter

BH1 52-9487 Tail Flick BH1 52-8570 Analgesia Meter Hot Plate Analgesia Meter with Footswitch

• Digital display of plate temperature • Digital timer with remote start stop • Accurate temperature control from 35°C to 65°C (±0.3°C) Hot Plate Analgesia Meter The Harvard Apparatus UK Hot Plate Analgesia Meter is a sophisticated • For rapid screening of analgesic drugs using rats temperature control and timing system, and has been designed to perform (as described by D’Amour and Smith) rapid and precise screening of the narcotic type analgesic drugs (Morphine, Codeine, etc.) according to the Eddy and Leimback hot plate test. This method Tail Flick Analgesia Meter evaluates the reaction time of mice when a heat stimulus is applied to the This meter measures a rat’s reaction time to radiant energy, from a 150 plantar surface. This reaction time increases when a central analgesic is watt light source. The beam is focused on its tail using a parabolic administered to the animal. This system can be used with both mice and rats. reflector. The energy of the light source can be adjusted and the display Utilizing a simple user interface the user can quickly and easily set up the indicates, as a percentage, how much energy is being utilized. required hot plate temperature and a large easy to read LED display shows the current temperature. An optical sensor is located underneath the focused light source. The rat should be positioned such that its tail obscures the focused light source The timer requires a single press of the Start / Stop Key to start and another from the sensor. When the system is started, either using the supplied press to stop, with reset automatically executed when timing is initiated. footswitch or front panel mounted start key the light source illuminates This function is also duplicated by a remote Start/Stop footswitch (supplied). and a timer starts counting in tenths of a second. When the rat’s tail The reaction time is again clearly displayed on a large LED display. flicks, indicating its pain threshold, it uncovers the sensor. This tail Using digital electronics, the hot plate temperature is constantly movement turns off the timer and light source. Reaction time can be read monitored and regulated to ensure the actual temperature and the desired directly from the display in seconds and tenths of a second. temperature accurately match. The system also monitors the heating characteristics of the system and uses this data to minimize heating A standard parallel port permits connection to a printer to record the trial overshoot, providing faster temperature stabilization. number, energy level and reaction time. A calibration facility allows the light source to be set to the desired level before commencing with the experiment. Specifications Mouse version is also available. Please contact Harvard Apparatus for details. Temperature Range 35° to 65°C Temperature Stability ±0.3°C Specifications Temperature Control Digital proportional PWM Lamp Intensity 150 W, adjustable between 0 and 100% in 1% Timer Digital readout in 0.1 sec increments increments Timer Range 0 to 9 mins 59 secs 9 tenths of a sec Timer Range 0 to 99 min 59.9 secs in 0.1 sec steps Remote Momentary make to start/stop Printer Interface Centronix parallel Remote Socket 6.35 mm 2 pole jack Lamp Heat Control Digital DC regulated Animal Container Two furnished, large round cylinders Dimensions, H x W x D 260 x 450 x 260 mm (10 x 18 x 10 in) Mains Supply Voltage 115 VAC / 230 VAC, 50/60 Hz (factory set) Weight 9 kg (19.8 lbs) Dimensions, H x W x D 128 x 275 x 293 mm (5 x 10.8 x 11.5 in) Weight 4.5 kg (9.9 lb) Product Order # Price $ Product Order # Price $ Tail Flick Analgesia Meter, 115 VAC, 60 Hz BH1 52-9487 3,853.00 Tail Flick Analgesia Meter, 240 VAC, 50 Hz BH1 52-9495 3,853.00 Hot Plate Analgesia Meter 110 to 115 VAC, 60 Hz BH1 52-8570 3,234.00 Hot Plate Analgesia Meter 220 to 230 VAC, 50 Hz BH1 52-8588 3,234.00 Connect With Us