Counting of RBC's and WBC's Using Image Processing Technique

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Research Article Volume 9 Issue No.4 Counting of RBC’s and WBC’s using Image Processing Technique Lokhande. T. P1, Salunke. P. B2, Shinde. P. T3, Chaugule. J. D4, Prof. Bhong. V. S5 Department of Electronics & Telecommunication Engineering SVERI’s COE, Pandharpur, India

Abstract: The measure of WBC and RBC Cells are very important for the doctor to diagnose various diseases such as anemia, leukemia etc. The number of blood cell counting is red blood cell and white blood cell count is then may be use to diagnose the patient as well detection of abnormalities like leukemia The use of image processing technique helps in improving the effectiveness of the in term of accuracy and time consumption.

Key words: RBCs,WBCs, Hough Transform

1. INTRODUCTION or. pngformate. These images are in RGB formate, these images are captured through digital microscope. Using digital In medical analysis blood cell count plays important role. microscopes or using a digital camera placed at the eye piece of Variations in the count of blood cells it causes many diseases in a microscope. the human body. For our health assessment and diagnosis of many disorders complete blood count is required. Rare increase Image Pre-Processing or decrease in cell count indicates that person has indispensable It is a technique of adjusting images, improving the quality of medical condition. The Complete Blood Count (CBC) is a blood image and making them suitable for the next step of process. test, extensively used to check various disorders such as Image pre-processing usually includes removing noise, contrast infections, allergies, problems with clotting, anemia, leukemia attractive, isolating regions and use of different color models etc. In order to perform CBC test, the blood film is stained and grayscale image. then imaged with a transmission light microscope. Here the analysis of the blood sample is done manually in order to count Image Enhancement. It is the techniques improves the quality, number of blood cells and also to identify the disorders in blood contrast and brightness characteristics of an image, also sharpen samples through a microscope. But it is a time consuming its details. Histogram plotting, histogram equalization, image process and also leads to undesirable human error. negation, image subtraction and filtering techniques are basic Image enhancement techniques. 2. PROPOSED SYSTEM Image Segmentation. Segmentation is used to separate object Image Acquisition: from the background. The method of segmentation used in this Image acquisition is making of digital microscope is interfaced counting process is Histogram Thresholding. to a computer and the microscopic images are obtained as Blood Cells Counting. The counting algorithm is used to digital images.We have obtained 50 images of blood cell. counting RBCs and WBCs. At the time of counting RBCs and Counting of blood cells: WBCs around the cell one circle are getting drawn. Counting the number of blood cells drawn gives the total number of blood cells in the image 2.3. Algorithm

Figure.1. System Block diagram

2.2 Block Diagram Description

Image Acquisition: In digital image acquisition image which is in the form of .jpeg Figure. 2. Algorithm for Proposed System

International Journal of Engineering Science and Computing, April 2019 21316 http://ijesc.org/ 3. METHODOLOGY:- different objects. Segmentation stage have no fear regarding Image segmentation is one of the first steps in image analysis the identity of the objects. The segmentation method is for object identification. The main aim is to acknowledge supported to identify the absolute homogeneity in grey levels constant regions at gap an image as happiness to totally at intervals the regions known.

4. RESULT:

(a) Original blood sample image, (b) green plane of an image,(c) therefore it can applied for differential white blood cell counting contrast adjusted image , (d) detected blood cells for diagnosing diseases based on different types of white blood (a) Original blood sample image:- This image is captured cell such as Monocyte, Lymphocytes, Neutrophills, Basophills through digital microscope after that capturing image it is and Eosinophils. converted into gray scale image . (b) green plane of an image:- This green plane image is used to 7. REFERENCE determined each pixel by the combination of red, green and blue intensities stored in each plane. [1]. L. Putzu and C. Ruberto, "White Blood Cells Identification (c) contrast adjusted image:- Contrast adjustment is used to and Classification from Leukemic Blood Image," in IWBBIO adjust image contrast by manupulating the histogram of international work- conference on bioinformatics and intensity values by setting the levels. biomedical engineering, Granzada, Spain, 2013. (d) detected blood cells:- The blood cells are detected by drawing the circle around the blood cell. According to this red [2]. Miss.Madhuri G. Bhamare, Prof. D.S Patil, “Automatic blood cells and white blood cells are counted. blood cell analysis by usingdigital image processing”, internationaljournal of engineering research and technology 5. CONCLUSİON:- (IJERT),vol 2 issue: 9, september 2013.

In this paper presents a software based solution for counting the [3].Akshaya P Sahastrabuddhe, “Counting of RBC and WBC blood cells. This Proposed method of cell counting is fast, cost using image processing”, IJRET:International Journal of effective and produces accurate result. This automated Research in Engineering and TechnologyVolume: 05 Issue: 05 methode can be easily implemented in medical facilities May-2016. anywhere with smallest investment in infrastructure.if patient having anaemia symptoms the blood cells counted by [4].Thejashwini M , M. C. Padma , “Counting of RBC’s and automated tester is not matching with the symptoms then WBC’s Using Image ProcessingTechnique”, International doctors prefers to try and do manual counting so as to diagnose Journal on Recent and Innovation Trends in Computing the problem.so Based on the result the proposed method was andCommunication Volume:3 Issue: 5 May 2015 able to produce an overall accuracy of 99.8% in automated [5].Karthikeyan. K .and K.. Brharama Neelima” Detection and system. Counting of Red Blood Cells using Hough Transform

Technique” International Journal of Advances in Engineering, 6. FUTURE SCOPE:- 2015.Issue 17Aug 2015.

The WBC and RBC image segmentation is the most important [6]. Esti Suryani, Wiharto, and NizomjonPolvonov” task for WBC and RBC classification in the automatic WBC Identification and Counting White Blood Cells and Red Blood and RBC different counting system. For future work, the results Cells using Image Processing Case Study of Leukemia” can be extended to separate the identified overlapping cell Volume 2.Nov 6

International Journal of Engineering Science and Computing, April 2019 21317 http://ijesc.org/