DOCSLIB.ORG

Stable Isotopes for Structural Biomolecular NMR Stable Isotopes for Structural Biomolecular NMR

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Stable Isotopes for Structural Biomolecular NMR Stable Isotopes for Structural Biomolecular NMR Cambridge Isotope Laboratories, Inc. Cambridge Isotope Laboratories, Inc. Stable Isotopes for Structural Biomolecular NMR Stable Isotopes for Structural Biomolecular NMR Bringing Structure to Life – CIL Isotopes Make it All Possible Cambridge Isotope Laboratories, Inc. 50 Frontage Road, Andover, MA 01810-5413 tel: +1- 978-749-8000 I fax: +1-978-749-2768 I 1-800-322-1174 (USA) I www.isotope.com 04 /11 Ordering Information The CIL Customer Service Department is open from 8:00 a.m. to 5:30 p.m. Eastern Standard Time. Orders may be placed by fax, email or via our website 24 hours a day. Phone: 1.800.322.1174 (North America) 1.978.749.8000 Fax: 1.978.749.2768 Email: [email protected] [email protected] (International) Website: www.isotope.com Copyright © 2011. All rights reserved. Cambridge Isotope Laboratories, Inc. Welcome Kevin Millis, Ph.D. Cambridge Isotope Laboratories, Inc. It is with great pride that we present CIL’s new Biomolecular NMR Product Catalog. In this catalog you will find a comprehensive listing of our isotope-enriched products used to study the structure and dynamics of protein and nucleic acids using nuclear magnetic resonance. We are pleased to present contributions written from some of the world’s most preeminent NMR spectroscopists. We are particularly pleased to present pieces written Cambridge Isotope Laboratories (CIL) is by Gerhard Wagner, Stanley Opella, Lewis Kay, Masatsune Kainosho, Leonid Brown & the world leader in the separation and Vladimir Ladizhansky, Hanudatta Atreya, and Linda Columbus & Daniel Fox. manufacture of stable isotopes and stable isotope-labeled compounds. Included in this catalog are a collection of CIL application notes relevant to magnetic For over 30 years CIL has remained resonance studies of biological molecules and systems. These application notes help the premier supplier of stable isotopes illustrate the usefulness of our products for both solution and solid-state NMR for NMR and MRS / MRI research applications. We would like to thank the many researchers who have written applications. These products include application notes that are included in this catalog. Also included in this catalog is a diverse line of RNA / DNA products, our most recent application note containing previously unpublished data that was minimal media reagents (carbohydrates, written by Drs. Leonid S. Brown and Vladimir Ladizhansky (Application Note 26). ammonium salts), cell-free expression These methods provide additional tools to study the structure and behavior of reagents and kits, free and protected membrane proteins. amino acids, as well as cell-growth media for eukaryotic and prokaryotic As new technologies and applications to study biopolymers using NMR advance, cell lines. Additionally, CIL offers a CIL continues to maintain a leadership role in developing new products for the comprehensive line of deuterated benefit of the entire biomolecular NMR community. CIL welcomes suggestions of solvents, detergents and buffers. our customers on additional products which would be beneficial to their research. Our products have been specifically It has been through our partnerships and close relationships with our customers designed and tested with the most over the past 30 years that we have been able to significantly expand our product discerning NMR spectroscopist and offering in order to assist the biomolecular NMR community in the advancement structural biologist in mind. CIL actively of their studies utilizing stable isotope-labeled compounds as a tool to structural supports the world’s NMR community NMR discovery. through meeting sponsorships and customer collaborations. All of us as CIL appreciate the collaborative relationships that we have with our customers. As the CIL Biomolecular NMR Product Manager, I look forward to continuing to work with you to expand our product offerings in ways that will best fulfill your needs. Kevin Millis, Ph.D. CIL Biomolecular NMR Product Manager tel: +1-978-749-8000 800-322-1174 (USA) fax: +1-978-749-2768 [email protected] 1 Cambridge Isotope Laboratories, Inc. Cambridge Isotope Laboratories’ area of expertise is the labeling of biochemical and organic compounds with highly enriched isotopes of carbon, hydrogen, nitrogen and oxygen. CIL is committed to providing the highest quality products coupled with unsurpassed service to researchers world- wide. With more than 10,000 products in our combined Research Products and Environmental Contaminant Standards inventory, we proudly offer the world’s largest range of stable isotope-labeled compounds. For more than 30 years, CIL products have contributed to advancements in drug discovery, environmental analysis, genomics and proteomics, and medical diagnostic research. We are proud of our history and our past success, which have been made possible by the talent and commitment of our employees. We look forward to continuing to service the scientific research community as we develop new stable isotope-labeled tools to aid researchers with new discoveries. 2tel: +1-978-749-8000 800-322-1174 (USA) fax: +1-978-749-2768 [email protected] Cambridge Isotope Laboratories, Inc. Table of Contents Table of Contents Ordering and Contact Information ................................................................ 4 Shipping, Product and Additional Information ...................................................... 5 Utility of Stable Isotopes in Structural Biology ...................................................... 6 Minimal Media Reagents ........................................................................ 8 a-Keto Acids, Methyl-Labeling Kits and Amino Acids ................................................ 10 Rich E. Coli Media ............................................................................. 11 Yeast Media and Reagents ..................................................................... 14 Insect Cell Media ............................................................................. 16 Mammalian Cell Media ........................................................................ 17 Cell-Free Protein Expression .................................................................... 18 Selective Amino Acid-Type Labeling .............................................................. 23 Free Amino Acids ............................................................................. 25 Protected Amino Acids ........................................................................ 28 Sparse Labeling Media and Reagents ............................................................. 30 Deuterated Detergents and Phospholipids for Membrane Proteins .................................... 32 Miscellaneous Protein Reagents ................................................................. 34 Protein NMR Standards ........................................................................ 36 Nucleic Acids ................................................................................. 38 Deuterated Buffers ........................................................................... 43 Application Notes Application Note 12: Optimization of BioExpress® 1000 Supplementation of M9 Cultures ...................... 45 Application Note 14: Efficient Uniform Isotope Labeling of Proteins Expressed in Baculovirus-Infected............ 47 Insect Cells Using BioExpress® 2000 (Insect Cell) Medium Application Note 15: Top Ten Tips for Producing 13C,15N Protein in Abundance .............................. 51 Application Note 16: Specific Isotopic Labeling of Methyl Groups Has Extended the Molecular.................. 55 Weight Limits for NMR Studies of Protein Structure and Dynamics Application Note 20: Effective Site-Specific Isotope Labeling (13C,15N Glycine; 13C,15N Phenylalanine; .............. 59 15N Tryptophan) Expression Optimization Using BioExpress® 2000 Media 13 Application Note 22: [2,3- C2]-Labeled Aromatic Residues as a Means to Improving Signal Intensities ............ 63 and Kick-Starting the Assignment of Membrane Protein by Solid-State MAS-NMR Application Note 25: Isotope Labeling of Alanine Methyl Groups on a Deuterated Background ................. 67 for NMR Studies of High-Molecular-Weight Proteins Application Note 26: Uniform Isotope Labeling of Eukaryotic Proteins in Methylotrophic Yeast ................. 71 for High-Resolution NMR Studies – Extension to Membrane Proteins NMR Solvent Data Chart ....................................................................... 75 Cover Credit: Computer artwork showing Caspase-9 molecule, courtesy of Dr. Mark J. Winter / Photo Researchers, Inc. tel: +1-978-749-8000 800-322-1174 (USA) fax: +1-978-749-2768 [email protected] 3 Cambridge Isotope Laboratories, Inc. Ordering and Contact Information Ordering and Contact Information Placing an Order Pricing Information and Terms of Sale Phone: 1-800-322-1174 (North America) or North American Orders 1-978-749-8000 (International) • All prices are in U.S. dollars. Any importation costs for Office hours are 8:00 a.m. to 5:30 p.m. Eastern international orders are not included. Please consult our Standard Time (EST) Customer Service Department for pricing information or Fax: 1-978-749-2768 packaging options. Email: [email protected] (North America) • When stock is available and subdivision is possible, we [email protected] (International) will accept orders for smaller than catalog amounts. Please E-commerce: Visit www.isotope.com to request a quote, place request a quotation as a quantity discount may apply. orders or submit technical
Load more

Recommended publications
  • Stationary Source Control Techniques Document for Fine Particulate Matter
    Stationary Source Control Techniques Document for Fine Particulate Matter EPA CONTRACT NO. 68-D-98-026 WORK ASSIGNMENT NO. 0-08 Prepared for: Mr. Kenneth Woodard Integrated Policy and Strategies Group (MD-15) Air Quality Strategies and Standards Division U.S. Environmental Protection Agency Research Triangle Park, North Carolina 27711 October 1998 Submitted by: EC/R Incorporated Timberlyne Center 1129 Weaver Dairy Road Chapel Hill, North Carolina 27514 Disclaimer This report has been reviewed by the Office of Air Quality Planning and Standards, U.S. Environmental Protection Agency, and has been approved for publication. Mention of trade names or commercial products is not intended to constitute endorsement or recommendation for use. Copies Copies of this document are available through the Library Services Office (MD-35), U.S. Environmental Protection Agency, Research Triangle Park, NC 27711; or from the National Technical Information Service, 5285 Port Royal Road, Springfield, VA 22161 (for a fee). This document can also be found on the Internet at the U.S. Environmental Protection Agency website (http:\\www.epa.gov/ttn/oarpg). ii CONTENTS TABLES .................................................................... ix FIGURES ................................................................... xi 1 INTRODUCTION .....................................................1-1 1.1 PURPOSE OF THIS DOCUMENT ..................................1-1 1.2 OTHER RESOURCES ............................................1-1 1.3 ORGANIZATION ...............................................1-1
    [Show full text]
  • ISOTEC® Stable Isotopes Biomolecular NMR Isotope Labeling Methods for Protein Dynamics Studies
    ISOTEC® Stable Isotopes Biomolecular NMR Isotope Labeling Methods for Protein Dynamics Studies Eric D. Watt and J. Patrick Loria, Ph.D. Department of Chemistry, Yale University, Kline Chemistry Laboratory, 225 Prospect Street, New Haven, CT 06520 Protein structure determination by solution NMR spectroscopy use of 15N-enriched minimal or nutrient-rich growth media that are has long relied on the uniform stable isotopic enrichment with readily available, allowing for easy sample preparation. Uniform 15N 13C and 15N to alleviate resonance overlap and to allow multiple labeling results in an isolated spin system (1H-15N) that lends itself well distance and angular restraints, at as many atomic sites as possible, to relaxation experiments. Every 15N position whether in the protein to facilitate computing the optimal three-dimensional structural backbone or sidechain is separated from another 15N atom by at least (1) 1 model. Recently, the optimization of these labeling techniques has two bonds. Therefore there are no JNN couplings that could lead to increased the range of protein sizes amendable to study, enhanced complicated multiexponential relaxation behavior, which would be the quality of three-dimensional structures, and simplified the analysis difficult to accurately measure and would cloud the interpretation of of experimental data.(2) Similarly, the field of protein dynamics has the associated motions. benefited from advances in isotopic labeling techniques that have However, 15N enrichment by itself does not provide a complete picture allowed researchers to study the motional properties of ever larger of the motions a protein undergoes. Nitrogen makes up only 1/3 of proteins over a broad range of timescales while more accurately the protein backbone and is only sparsely populated in the sidechains describing the protein motions.
    [Show full text]
  • SKIMMER 2014-4.Pdf
    SKIMMER 2014-4 ddd3 The Wayfarer United States Wayfarer Association Fall 2014-4 As Few Words As Possible So, thank you all. I accept, reassured in the knowledge From Your Vice Commodore that bad publicity is better than no publicity at all. Chip Cunningham, W1321, Solje What can go wrong? Right off the bat I’ve felt “Are we going to keep sailing together?” Nick asked. comfortable with the Wayfarer community, but I’m “Of course,” I answered without giving it much still on the steep part of the learning curve for thought. Wayfarer boats and sailing. I hope my perspective is an interesting way to promote the U.S. Wayfarer “You know, as helm I decide what you do, right?” Association because sailing these boats is such a good “What are you getting at, Nick?” reason for us to keep getting together. “You are going to take over from me as Vice Commodore.” And so began another day of sailing with Nick. COMMODORE COMMENTS Jim Heffernan, W1066, W2458 An organization such as the USWA is made up of volunteers who take on projects and events and those that serve as officers or committee members. The Wayfarer Class and the USWA have been very fortunate since the mid sixties to have Nick Seraphinoff involved in both areas as a skipper of numerous Wayfarers, organizer of regattas, importer of new Wayfarers, Class promoter and until recently, Vice-Commodore. The Class continues to grow throughout North America due to his efforts over the past seven years. As he hands over the sword of office and the tri-cornered chapeau to Chip Cunningham, we Chip contemplates new duties as Vice Commodore.
    [Show full text]
  • A Roadmap for Interpreting 13C Metabolite Labeling Patterns from Cells
    Available online at www.sciencedirect.com ScienceDirect 13 A roadmap for interpreting C metabolite labeling patterns from cells 1,2 3,* 4,* Joerg M Buescher , Maciek R Antoniewicz , Laszlo G Boros , 5,* 6,* 7,* Shawn C Burgess , Henri Brunengraber , Clary B Clish , 8,* 9,* 10,* Ralph J DeBerardinis , Olivier Feron , Christian Frezza , 1,2,* 11,* 12,* Bart Ghesquiere , Eyal Gottlieb , Karsten Hiller , 13,* 14,* 15,* Russell G Jones , Jurre J Kamphorst , Richard G Kibbey , 16,* 17,* 18,* Alec C Kimmelman , Jason W Locasale , Sophia Y Lunt , 11,* 19,* 20,* Oliver DK Maddocks , Craig Malloy , Christian M Metallo , 21,22,* 23,24,* Emmanuelle J Meuillet , Joshua Munger , 25,* 26,* 27,28,* Katharina No¨ h , Joshua D Rabinowitz , Markus Ralser , 29,* 30,* 31,* Uwe Sauer , Gregory Stephanopoulos , Julie St-Pierre , 32,* 33,* Daniel A Tennant , Christoph Wittmann , 34,35,* 11,* Matthew G Vander Heiden , Alexei Vazquez , 11,* 36,37,* 29,* Karen Vousden , Jamey D Young , Nicola Zamboni and 1,2 Sarah-Maria Fendt 13 Measuring intracellular metabolism has increasingly led to Goodman Cancer Research Centre, Department of Physiology, McGill 13 University, Montreal, QC, Canada important insights in biomedical research. C tracer analysis, 14 13 Institute of Cancer Sciences, University of Glasgow, Glasgow, UK although less information-rich than quantitative C flux 15 Internal Medicine, Cellular and Molecular Physiology, Yale University analysis that requires computational data integration, has been School of Medicine, New Haven, CT, USA 16 established as a time-efficient method to unravel relative Division of Genomic Stability and DNA Repair, Department of Radiation Oncology, Dana-Farber Cancer Institute, Boston, MA, USA pathway activities, qualitative changes in pathway 17 Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA contributions, and nutrient contributions.
    [Show full text]
  • Calculation of Neutron Cross Sections on Isotopes of Yttrium and Zirconium
    pp. LA-7789-MS Informal Report Calculation of Neutron Cross Sections on Isotopes of Yttrium and Zirconium co O CO 5 • . LOS ALAMOS SCIENTIFIC LABORATORY Rpst Office Bex 1663 Los Alamos. New Mexico 37545 A LA-7789-MS Informal Report UC-34c Issued: April 1979 Calculation of Neutron Cross Sections on Isotopes of Yttrium and Zirconium E. D. Arthur - NOTICE- Tim report wt piepited u an account of work sponsored by the United Stales Government. Neither the United States nor the United Statci Department of Energy, nor any of their empioyeet, nor any of their contractor*, subcontractor!, or their employees, nukes any warranty, express or implied, ot astumes any legal liability 01 responsibility foi the accuiacy, completeness or luefulnets of any Information, apparatus, product or piocett ductoied.oi ^presents that iti uie would not infringe privately owned rights. CALCULATION OF NEUTRON CROSS SECTIONS ON ISOTOPES OF YTTRIUM AND ZIRCONIUM by E. D. Arthur ABSTRACT Multistep Hauser-Feshbach calculations with preequilibrium corrections have been made for neutron-induced reactions on yttrium and zirconium isotopes between 0.001 and 20 MeV. Recent- ly new neutron cross-section data have been measured for unstable isotopes of these elements. These data, along with results from charged-particle simulation of neutron reactions, provide unique opportunities under which to test nuclear-model techniques and parameters in this mass region. We have performed a complete and consistent analysis of varied neutron reaction types using input parameters determined independently from additional neutron and charged-particle data. The overall agreement between our calcula- tions and a wide variety of experimental results available for these nuclei leads to increased confidence in calculated cross sections made where data are incomplete or lacking.
    [Show full text]
  • Isotope Distributions
    Isotope distributions This exposition is based on: • R. Martin Smith: Understanding Mass Spectra. A Basic Approach. Wiley, 2nd edition 2004. [S04] • Exact masses and isotopic abundances can be found for example at http: //www.sisweb.com/referenc/source/exactmaa.htm or http://education. expasy.org/student_projects/isotopident/htdocs/motza.html • IUPAC Compendium of Chemical Terminology - the Gold Book. http:// goldbook.iupac.org/ [GoldBook] • Sebastian Bocker,¨ Zzuzsanna Liptak:´ Efficient Mass Decomposition. ACM Symposium on Applied Computing, 2005. [BL05] • Christian Huber, lectures given at Saarland University, 2005. [H05] • Wikipedia: http://en.wikipedia.org/, http://de.wikipedia.org/ 10000 Isotopes This lecture addresses some more combinatorial aspect of mass spectrometry re- lated to isotope distributions and mass decomposition. Most elements occur in nature as a mixture of isotopes. Isotopes are atom species of the same chemical element that have different masses. They have the same number of protons and electrons, but a different number of neutrons. The main ele- ments occurring in proteins are CHNOPS. A list of their naturally occurring isotopes is given below. Isotope Mass [Da] % Abundance Isotope Mass [Da] % Abundance 1H 1.007825 99.985 16O 15.994915 99.76 2H 2.014102 0.015 17O 16.999131 0.038 18O 17.999159 0.20 12C 12. (exact) 98.90 13C 13.003355 1.10 31P 30.973763 100. 14N 14.003074 99.63 32S 31.972072 95.02 15N 15.000109 0.37 33S 32.971459 0.75 34S 33.967868 4.21 10001 Isotopes (2) Note that the lightest isotope is also the most abundant one for these elements.
    [Show full text]
  • Large D/H Variations in Bacterial Lipids Reflect Central Metabolic Pathways
    Large D/H variations in bacterial lipids reflect central metabolic pathways Xinning Zhanga, Aimee L. Gillespieb, and Alex L. Sessionsa,b,1 aEnvironmental Science and Engineering Program and bDivision of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125 This Feature Article is part of a series identified by the Editorial Board as reporting findings of exceptional significance. Edited by John M. Hayes, Woods Hole Oceanographic Institution, Woods Hole, MA, and approved May 29, 2009 (received for review March 19, 2009) Large hydrogen-isotopic (D/H) fractionations between lipids and compositions. Jones et al. (17) measured fatty acids extracted growth water have been observed in most organisms studied to from coastal marine particulate organic matter (POM) and date. These fractionations are generally attributed to isotope found ␦D values ranging from Ϫ73 to Ϫ237‰. Measurements effects in the biosynthesis of lipids, and are frequently assumed to of lipids from marine sediments have extended this range from be approximately constant for the purpose of reconstructing cli- Ϫ32 to Ϫ348‰ for lipids with n-alkyl skeletons and Ϫ148 to matic variables. Here, we report D/H fractionations between lipids Ϫ469‰ for those with isoprenoid skeletons (18). Because the and water in 4 cultured members of the phylum Proteobacteria, lipids measured by both studies likely derive from marine and show that they can vary by up to 500‰ in a single organism. organisms inhabiting seawater of essentially constant ␦D value The variation cannot be attributed to lipid biosynthesis as there is (Ϸ0‰), such differences cannot be due to varying environmen- no significant change in these pathways between cultures, nor can tal water.
    [Show full text]
  • DOE-STD-1027-2018, Hazard Categorization of DOE Nuclear
    NOT MEASUREMENT SENSITIVE DOE-STD-1027-2018 November 2018 DOE STANDARD HAZARD CATEGORIZATION OF DOE NUCLEAR FACILITIES U.S. Department of Energy Washington, DC 20585 DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited. Available to the public on the DOE Technical Standards Program website at www.standards.doe.gov ii FOREWORD 1. This Department of Energy Standard has been approved to be used by DOE, including the National Nuclear Security Administration, and their contractors. 2. Beneficial comments (recommendations, additions, and deletions), as well as any pertinent data that may be of use in improving this document should be emailed to [email protected] or addressed to: Office of Nuclear Safety (AU-30) Office of Environment, Health, Safety and Security U.S. Department of Energy 19901 Germantown Road Germantown, MD 20874 3. Title 10 of the Code of Federal Regulations Part 830, Nuclear Safety Management, Subpart B, Safety Basis Requirements, establishes safety basis requirements for hazard category 1, 2, or 3 DOE nuclear facilities. This Standard provides an acceptable methodology to “[c]ategorize the facility consistent with DOE-STD-1027-92 (“Hazard Categorization and Accident Analysis Techniques for compliance with DOE Order 5480.23, Nuclear Safety Analysis Reports,” Change Notice 1, September 1997),” as required by 10 CFR Section 202(b)(3). 4. The goal of this revised Standard is to maintain consistency with the methodology of DOE- STD-1027-92, CN1, while providing clearer criteria and guidance to support effective and consistent hazard categorization based upon more recent input values and lessons learned in implementing DOE-STD-1027-92, CN1.
    [Show full text]
  • ABSTRACT Bucci, John P. Blue Crab Trophic Dynamics
    ABSTRACT Bucci, John P. Blue crab trophic dynamics: Stable isotope analyses in two North Carolina estuaries. Eutrophication is increasing in estuaries as a result of anthropogenic activity along the land-sea margin. Human activities contribute large amounts of nitrogen and carbon compounds to watersheds, resulting in changes in resource availability through alteration of biogeochemical cycles and habitat destruction. Although the effects of poor water quality on lower trophic level biota is well understood, the impact of nutrient waste on upper trophic levels, such as blue crabs (Callinectes sapidus), has not been well studied. Stable nitrogen (δ15N) and carbon (δ13C) isotope ratios can provide time and space integrated information about feeding relationships and energy flow through food webs. An isotopic comparison of the trophic structure of two North Carolina estuaries was undertaken to understand the impacts of anthropogenic runoff on blue crab interactions and feeding habits. This study examined isotopic signatures of primary producers, as well as blue crab and their bivalve prey (Rangia cuneata & Corbicula fluminea) as indicators of potential changes in food web relationships in response to eutrophication. The Neuse River Estuary is an “impacted” system that experiences high nitrogen loading and drains areas of urban development, row crop agriculture, and concentrated animal operations. The Alligator River Estuary by comparison, is designated as a “less-impacted” system in this study. The Alligator River Estuary is classified as having "Outstanding Resource Waters” and low nutrient loading. In each estuary, samples were collected in the upper, middle and lower regions of the river. Bivalves collected from the Neuse River Estuary yielded a significant difference (p<0.0001) in mean nitrogen isotopic composition of tissue (10.4‰ ± 0.82; N=66) compared to the bivalves collected from the Alligator River Estuary (6.4‰ ± 0.63; N=45).
    [Show full text]
  • 2019 One Design Classes and Sailor Survey
    2019 One Design Classes and Sailor Survey [email protected] One Design Classes and Sailor Survey One Design sailing is a critical and fundamental part of our sport. In late October 2019, US Sailing put together a survey for One Design class associations and sailors to see how we can better serve this important constituency. The survey was sent via email, as a link placed on our website and through other USSA Social media channels. The survey was sent to our US Sailing members, class associations and organizations, and made available to any constituent that noted One-Design sailing in their profile. Some interesting observations: • Answers are based on respondents’ perception of or actual experience with US Sailing. • 623 unique comments were received from survey respondents and grouped into “Response Types” for sorting purposes • When reviewing data, please note that “OTHER” Comments are as equally important as those called out in a specific area, like Insurance, Administration, etc. • The majority of respondents are currently or have been members of US Sailing for more than 5 years, and many sail in multiple One-Design classes • About 1/5 of the OD respondents serve(d) as an officer of their primary OD class; 80% were owner/drivers of their primary OD class; and more than 60% were members of their primary OD class association. • Respondents to the survey were most highly concentrated on the East and West coasts, followed by the Mid- West and Texas – though we did have representation from 42 states, plus Puerto Rico and Canada. • Most respondents were male.
    [Show full text]
  • Selective Isotope Labeling Methods for Protein Structural Studies
    Cambridge Isotope Laboratories, Inc. www.isotope.com Biomolecular NMR Selective Isotope-Labeling Methods for Protein Structural Studies Hanudatta S. Atreya, PhD NMR Research Centre, Indian Institute of Science, Bangalore, India One of the major contributing factors to the rapid advance dynamic studies. Selective amino acid-type labeling also aids in of biomolecular NMR spectroscopy is the emergence of different sequence-specific resonance assignments by helping to identify isotope labeling methods. Recent developments in biotechnology resonances which are otherwise buried in the crowded regions have made it easier and economical to introduce 13C,15N and 2H of 2D and 3D NMR spectra. However, a disadvantage of this into proteins and nucleic acids. At the same time, there has been method is the possible mis-incorporation of 15N label in undesired an explosion in the number of NMR experiments that utilize such amino acids (also called as “isotope scrambling”).3 This happens isotope-labeled samples. Thus, a combination of isotopic labeling due to metabolic conversion of one amino acid to another in and multidimensional, multinuclear experiments has significantly the bio-synthetic pathway of the cell. The problem becomes expanded the range of problems in structural biology amenable more severe for amino acids higher up or intermediates in the to NMR. metabolic pathway such as Asp, Glu and Gln (See Figure 2 showing the biosynthetic pathway in E. coli ). For those which Isotope labeling in proteins can be broadly classified into four are end-products in the production pipeline (Ala, Arg, Asn, Cys, categories: Uniform, amino acid-type selective, site-specific His, Ile, Lys, Met, Pro and Trp) isotope scrambling is minimal and and random / fractional labeling.
    [Show full text]
  • Los Alamos NATIONAL LABORATORY
    — . .. ,. ~ - /3~5Y” m5 ~“3 CIC-14 REPORT CQUECTW REPRODUCTION COPY Measurement and Accounting of the Minor Adinides Produced in Nuclear Power Reactors Los Alamos NATIONAL LABORATORY .i,os Alarnos National Laboratory is operated by the University of Cal~ornia for the United States Department of Energy under contract W-7405-ENG-36. Etlifeci by Paul IV. Fknriksen, Group ClC-l Prepared by Celirza M. CMz, Group lVIS-5 This work was supported by the U.S. Department of Energy, Ofice of lVonprol~eration and National Security, Ofice of Safeguards and Security, An Ajirmativc AcfionfEqual Opporfunify Employer This report waspreparedasan accountof worksponsoredby an agencyof theUnited States Govemrnent. NeitherTheRegentsof fhe Universityof Cal@rnia,the United Stafes Government norany agencythereof,norany of theiremployees,makesany warranty,express or implied,or assumesany legalliabilityor responsibilify~ortheaccuracy,completeness, or usefulness of any information, apparatus, product, or process disclosed,orrepresentsthat its use wouldnof infringe privately owned rights. Referenceherein to any speczfic commercial product, process, or seru”ce by trade name, trademark, manufacturer, or otherwise, doesnot necessarily constitute or imply its endorsement, recommendation, or favoring by The Regents of the University of California, fhe LInifedStates Government, or any agency thereof. The views and opinions of aufhors expressed herein do nof necessarily sfate or r~ect those of The Regents of the University of Calt@nia, the Unifed Sfates Government, or any agency thereo$ The Los A[amos National Laboratory strongly supports academic freedom and a researcher’s right to publish; therefore, the .!..aboratory as an institution doesnot endorse the viewpoint of a publication or guarantee its technical correctness. LA-13054-MS UC-700 Issued: January 1996 Measurement and Accounting of the Minor Actinides Produced in Nuclear Power Reactors J.
    [Show full text]