Cambridge Isotope Laboratories, Inc. Cambridge Isotope Laboratories, Inc. Stable Isotopes for Structural Biomolecular NMR Stable Isotopes for Structural Biomolecular NMR Bringing Structure to Life – CIL Isotopes Make it All Possible Cambridge Isotope Laboratories, Inc. 50 Frontage Road, Andover, MA 01810-5413 tel: +1- 978-749-8000 I fax: +1-978-749-2768 I 1-800-322-1174 (USA) I www.isotope.com 04 /11 Ordering Information The CIL Customer Service Department is open from 8:00 a.m. to 5:30 p.m. Eastern Standard Time. Orders may be placed by fax, email or via our website 24 hours a day. Phone: 1.800.322.1174 (North America) 1.978.749.8000 Fax: 1.978.749.2768 Email: [email protected] [email protected] (International) Website: www.isotope.com Copyright © 2011. All rights reserved. Cambridge Isotope Laboratories, Inc. Welcome Kevin Millis, Ph.D. Cambridge Isotope Laboratories, Inc. It is with great pride that we present CIL’s new Biomolecular NMR Product Catalog. In this catalog you will find a comprehensive listing of our isotope-enriched products used to study the structure and dynamics of protein and nucleic acids using nuclear magnetic resonance. We are pleased to present contributions written from some of the world’s most preeminent NMR spectroscopists. We are particularly pleased to present pieces written Cambridge Isotope Laboratories (CIL) is by Gerhard Wagner, Stanley Opella, Lewis Kay, Masatsune Kainosho, Leonid Brown & the world leader in the separation and Vladimir Ladizhansky, Hanudatta Atreya, and Linda Columbus & Daniel Fox. manufacture of stable isotopes and stable isotope-labeled compounds. Included in this catalog are a collection of CIL application notes relevant to magnetic For over 30 years CIL has remained resonance studies of biological molecules and systems. These application notes help the premier supplier of stable isotopes illustrate the usefulness of our products for both solution and solid-state NMR for NMR and MRS / MRI research applications. We would like to thank the many researchers who have written applications. These products include application notes that are included in this catalog. Also included in this catalog is a diverse line of RNA / DNA products, our most recent application note containing previously unpublished data that was minimal media reagents (carbohydrates, written by Drs. Leonid S. Brown and Vladimir Ladizhansky (Application Note 26). ammonium salts), cell-free expression These methods provide additional tools to study the structure and behavior of reagents and kits, free and protected membrane proteins. amino acids, as well as cell-growth media for eukaryotic and prokaryotic As new technologies and applications to study biopolymers using NMR advance, cell lines. Additionally, CIL offers a CIL continues to maintain a leadership role in developing new products for the comprehensive line of deuterated benefit of the entire biomolecular NMR community. CIL welcomes suggestions of solvents, detergents and buffers. our customers on additional products which would be beneficial to their research. Our products have been specifically It has been through our partnerships and close relationships with our customers designed and tested with the most over the past 30 years that we have been able to significantly expand our product discerning NMR spectroscopist and offering in order to assist the biomolecular NMR community in the advancement structural biologist in mind. CIL actively of their studies utilizing stable isotope-labeled compounds as a tool to structural supports the world’s NMR community NMR discovery. through meeting sponsorships and customer collaborations. All of us as CIL appreciate the collaborative relationships that we have with our customers. As the CIL Biomolecular NMR Product Manager, I look forward to continuing to work with you to expand our product offerings in ways that will best fulfill your needs. Kevin Millis, Ph.D. CIL Biomolecular NMR Product Manager tel: +1-978-749-8000 800-322-1174 (USA) fax: +1-978-749-2768 [email protected] 1 Cambridge Isotope Laboratories, Inc. Cambridge Isotope Laboratories’ area of expertise is the labeling of biochemical and organic compounds with highly enriched isotopes of carbon, hydrogen, nitrogen and oxygen. CIL is committed to providing the highest quality products coupled with unsurpassed service to researchers world- wide. With more than 10,000 products in our combined Research Products and Environmental Contaminant Standards inventory, we proudly offer the world’s largest range of stable isotope-labeled compounds. For more than 30 years, CIL products have contributed to advancements in drug discovery, environmental analysis, genomics and proteomics, and medical diagnostic research. We are proud of our history and our past success, which have been made possible by the talent and commitment of our employees. We look forward to continuing to service the scientific research community as we develop new stable isotope-labeled tools to aid researchers with new discoveries. 2tel: +1-978-749-8000 800-322-1174 (USA) fax: +1-978-749-2768 [email protected] Cambridge Isotope Laboratories, Inc. Table of Contents Table of Contents Ordering and Contact Information ................................................................ 4 Shipping, Product and Additional Information ...................................................... 5 Utility of Stable Isotopes in Structural Biology ...................................................... 6 Minimal Media Reagents ........................................................................ 8 a-Keto Acids, Methyl-Labeling Kits and Amino Acids ................................................ 10 Rich E. Coli Media ............................................................................. 11 Yeast Media and Reagents ..................................................................... 14 Insect Cell Media ............................................................................. 16 Mammalian Cell Media ........................................................................ 17 Cell-Free Protein Expression .................................................................... 18 Selective Amino Acid-Type Labeling .............................................................. 23 Free Amino Acids ............................................................................. 25 Protected Amino Acids ........................................................................ 28 Sparse Labeling Media and Reagents ............................................................. 30 Deuterated Detergents and Phospholipids for Membrane Proteins .................................... 32 Miscellaneous Protein Reagents ................................................................. 34 Protein NMR Standards ........................................................................ 36 Nucleic Acids ................................................................................. 38 Deuterated Buffers ........................................................................... 43 Application Notes Application Note 12: Optimization of BioExpress® 1000 Supplementation of M9 Cultures ...................... 45 Application Note 14: Efficient Uniform Isotope Labeling of Proteins Expressed in Baculovirus-Infected............ 47 Insect Cells Using BioExpress® 2000 (Insect Cell) Medium Application Note 15: Top Ten Tips for Producing 13C,15N Protein in Abundance .............................. 51 Application Note 16: Specific Isotopic Labeling of Methyl Groups Has Extended the Molecular.................. 55 Weight Limits for NMR Studies of Protein Structure and Dynamics Application Note 20: Effective Site-Specific Isotope Labeling (13C,15N Glycine; 13C,15N Phenylalanine; .............. 59 15N Tryptophan) Expression Optimization Using BioExpress® 2000 Media 13 Application Note 22: [2,3- C2]-Labeled Aromatic Residues as a Means to Improving Signal Intensities ............ 63 and Kick-Starting the Assignment of Membrane Protein by Solid-State MAS-NMR Application Note 25: Isotope Labeling of Alanine Methyl Groups on a Deuterated Background ................. 67 for NMR Studies of High-Molecular-Weight Proteins Application Note 26: Uniform Isotope Labeling of Eukaryotic Proteins in Methylotrophic Yeast ................. 71 for High-Resolution NMR Studies – Extension to Membrane Proteins NMR Solvent Data Chart ....................................................................... 75 Cover Credit: Computer artwork showing Caspase-9 molecule, courtesy of Dr. Mark J. Winter / Photo Researchers, Inc. tel: +1-978-749-8000 800-322-1174 (USA) fax: +1-978-749-2768 [email protected] 3 Cambridge Isotope Laboratories, Inc. Ordering and Contact Information Ordering and Contact Information Placing an Order Pricing Information and Terms of Sale Phone: 1-800-322-1174 (North America) or North American Orders 1-978-749-8000 (International) • All prices are in U.S. dollars. Any importation costs for Office hours are 8:00 a.m. to 5:30 p.m. Eastern international orders are not included. Please consult our Standard Time (EST) Customer Service Department for pricing information or Fax: 1-978-749-2768 packaging options. Email: [email protected] (North America) • When stock is available and subdivision is possible, we [email protected] (International) will accept orders for smaller than catalog amounts. Please E-commerce: Visit www.isotope.com to request a quote, place request a quotation as a quantity discount may apply. orders or submit technical