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Regulatory T cell research Unique kits for cell isolation Harmonized cell culture and expansion tools Convenient functional assay tools Germany/Austria/ Benelux France Nordics and Baltics South Korea Switzerland Miltenyi Biotec B.V. Miltenyi Biotec SAS Miltenyi Biotec Norden AB Miltenyi Biotec Korea Co., Ltd Reliable flow cytometry analysis Miltenyi Biotec GmbH Schipholweg 68 H 10 rue Mercoeur Scheelevägen 17 Arigi Bldg. 8F Friedrich-Ebert-Straße 68 2316 XE Leiden 75011 Paris, France 223 70 Lund 562 Nonhyeon-ro 51429 Bergisch Gladbach The Netherlands Phone +33 1 56 98 16 16 Sweden Gangnam-gu Germany [email protected] Fax +33 1 56 98 16 17 [email protected] Seoul 06136, South Korea Phone +49 2204 8306-0 Customer service [email protected] Customer service Sweden Phone +82 2 555 1988 Fax +49 2204 85197 The Netherlands Phone 0200-111 800 Fax +82 2 555 8890 [email protected] Phone 0800 4020120 Italy Fax 046-280 72 99 [email protected] Fax 0800 4020100 Miltenyi Biotec S.r.l. 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No. 319, Xianxia Road Tokyo 135-0041, Japan #28-06 to 28-08 Shaw Tower Miltenyi Biotec Ltd. Unit 16 A, 2 Eden Park Drive Changning District Phone +81 3 5646 8910 Singapore 189702 Almac House, Church Lane Macquarie Park NSW 2113 200051 Shanghai, P.R. China Fax +81 3 5646 8911 Phone +65 6238 8183 Bisley, Surrey GU24 9DR, UK Australia Phone +86 21 62351005 [email protected] Fax +65 6238 0302 Phone +44 1483 799 800 Phone +61 2 8877 7400 Fax +86 21 62350953 [email protected] Fax +44 1483 799 811 Fax +61 2 9889 5044 [email protected] [email protected] [email protected] www.miltenyibiotec.com Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS® GMP Products are for research use and ex vivo cell culture processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative. MACS GMP Products are manufactured and tested under a quality system certified to ISO 13485 and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials. The CliniMACS® System components, including Reagents, Tubing Sets, Instruments, and PBS/EDTA Buffer, are designed, manufactured and tested under a quality system certified to ISO 13485. In the EU, the CliniMACS System components are available as CE-marked medical devices for their respective intended use, unless otherwise stated. The CliniMACS Reagents and Biotin Conjugates are intended for in vitro use only and are not designated for therapeutic use or direct infusion into patients. The CliniMACS Reagents in combination with the CliniMACS System are intended to separate human cells. Miltenyi Biotec as the manufacturer of the CliniMACS System does not give any recommendations regarding the use of separated cells for therapeutic purposes and does not make any claims regarding a clinical benefit. For the manufacturing and use of target cells in humans the national legislation and regulations - e.g. for the EU the Directive 2004/23/EC ("human tissues and cells"), or the Directive 2002/98/EC ("human blood and blood components") - must be followed. Thus, any clinical application of the target cells is exclusively within the responsibility of the user of a CliniMACS System. In the US, the CliniMACS CD34 Reagent System, including the CliniMACS Plus Instrument, CliniMACS CD34 Reagent, CliniMACS Tubing Sets TS and LS, and the CliniMACS PBS/EDTA Buffer, is FDA approved; all other products of the CliniMACS Product Line are available for use only under an approved Investigational New Drug (IND) application or Investigational Device Exemption (IDE). CliniMACS MicroBeads are for research use only and not for human therapeutic or diagnostic use. CliniMACS, CliniMACS Prodigy, TexMACS, autoMACS, MultiMACS, gentleMACS, MACSmix, MACSiBead, MACSiMAG, REAfinity, VioBlue, VioBright, VioGreen, Vio, MACS, and the MACS logo are registered trademarks or trademarks of Miltenyi Biotec GmbH and/or its affiliates in countries worldwide. Copyright © 2016 Miltenyi Biotec GmbH and/or its affiliates. All rights reserved. 130-093-700.05 Introduction The complete workflow for regulatory T cell research Regulatory T (Treg) cells are a subpopulation of T cells, which are characterized by their immune suppressive function. They are essential to maintain tolerance to self-antigens and to suppress overwhelming reactions against pathogens. Due to their outstanding role within the immune system, Treg cells are of paramount interest for immunology research and with our broad portfolio of products we aim to provide a complete workflow solution for the investigation of Treg cells. We support you during all steps of your experiments. • Broad Treg cell product portfolio to fit your research needs • Optimized tools for your Treg cell separation, expansion and suppression assays • Standardized cell culture medium and cytokines up to GMP grade for optimal Treg culture conditions 2 Table of contents • Viable cell suspensions through gentle tissue dissociation gentleMACS™ Dissociators • Fast and reliable, user-independent results SAMPLE PREPARATION 5 • Fast and efficient isolation of Treg cells from human whole blood or PBMCs MACS® Technology • Isolation of mouse and non-human primate Treg cells CELL SEPARATION 6 • Cell culture products perfectly suited for in vitro TexMACS™ Medium expansion and characterization of Treg cells CELL CULTURE 12 • Easy-to-use Treg Detection Kits • Broad portfolio of recombinantly engineered REAfinity REAfinity™ Antibodies Antibodies for the characterization of Treg cell subsets FLOW CYTOMETRY 14 3 Regulatory T cell workflow Research Clinical gentleMACS™ Dissociators • Tissue Dissociation Kits SAMPLE PREPARATION Leukapheresis • MACSxpress® MACSxpress® Separator Treg Isolation Kit, human • CD4+CD25+ Regulatory T Cell Isolation Kit, human • CliniMACS® CD25 Reagent* • CD4+CD25+CD127dim/– • CliniMACS CD8 Reagent* Regulatory T Cell • CliniMACS CD19 Reagent* Isolation Kit, human • CliniMACS Anti-Biotin • CD4+CD25+CD45RA MACS® MultiStand Reagent* CliniMACS® Systems Regulatory T Cell Isolaton Kit, human • CD25+CD49– Regulatory • MACS® GMP Fluorescent T Cell Isolation Kit, human Antibodies*: • CD4+CD25+ Regulatory - CD25-PE-Biotin T Cell Isolation Kit, mouse - CD4-VioBlue - CD127-APC + + • CD4 CD25 Regulatory - CD45RA-FITC T Cell Isolation Kit, - CD25-PE autoMACS® pro Separator non-human primate CELL SEPARATION - CD4-PerCP MACSQuant® Tyto™ • TexMACS™ GMP Medium* • MACS® GMP ExpAct Treg Kit* • MACS GMP Recombinant • TexMACS™ Medium, Human IL-2* research grade • MACS GMP Rapamycin* • Treg Expansion Kit, human • MACS GMP Cell Expansion • Treg Expansion Kit, mouse Bags* • Treg Suppression • MACS GMP Cell MACS Cell Culture Inspector, human CELL CULTURE Differentiation Bags* MACS® GMP Products • Treg Detection Kit (CD4/CD25/CD127), human • Treg Detection Kit (CD4/ • Treg Detection Kit (CD4/ CD25/ CD127), human (RUO) CD25/FoxP3), human • Treg Detection Kit (CD4/ • Treg Detection Kit (CD4/ CD25/FoxP3), human (RUO) CD25/FoxP3), mouse NEW: REAfinity™ Antibodies NEW: REAfinity™ Antibodies (RUO) for regulatory for regulatory T Cell detection T cell detection and REAfinity™ Antibodies and characterization FLOW CYTOMETRY characterization MACSQuant® Portfolio RUO=Research Use Only * For complete regulatory and legal notices please refer to the back page of the brochure. 4 SAMPLE PREPARATION Start smart The secret to success lies in your starting material gentleMACS™ Dissociators gentleMACS™ Tubes Experience the gentleMACS™ Portfolio of versatile benchtop Tubes for the gentleMACS™ Dissociators, precision engineered tissue dissociators that can process up to eight samples in to guarantee highest quality tissue dissociation or homo- parallel. They come with programmable protocol settings genization. and optional temperature regulation to ensure • gentleMACS C Tubes: use the purple C Tubes for high standardized tissue dissociation. yields of viable single cells • Gentle: viable single-cell suspensions with preserved • gentleMACS M Tubes: choose the orange M Tubes to epitopes thoroughly homogenize cells for, e.g., molecular analyses • Reproducible: pre-defined programs for standardized tissue processing • Fast: processing of up to eight samples in
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