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On Enhancing Nicotinamide Adenine Dinucleotide (Nad) Activity in Living Systems

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On Enhancing Nicotinamide Adenine Dinucleotide (Nad) Activity in Living Systems Experimental ON ENHANCING NICOTINAMIDE ADENINE DINUCLEOTIDE (NAD) ACTIVITY IN LIVING SYSTEMS Michael J. Kohane & William A. Tiller, Ph.D. ABSTRACT Nicotinamide Adenine Dinucleotide (NAD) is a key molecule in intermediary metabolism and for the flow of energy in biological systems. It has been shown that a significant association exists in Drosophila melanogaster, the fruit fly, between larval development time, NAD and Adenosine Triphosphate (ATP)/Adenosine Diphosphate (ADP) ratio. Furthermore, variation in NAD concentration has been associated with both AIDS and Alzheimer's disease in humans. Recent studies have favorably implicated NAD as a medication fot patients suffering from both dementia of the Alzheimer type and HIV-l infection. In this paper, we have examined the possibility that both EMFs and intention-augmented EMFs significantly affect (1) the activity of NAD in solution over time and (2) the condition of the water used for preparing NAD solutions to be subsequently utilized for in vitro energy assays in D. melanogaster. Here, we show that NAD activity and the (ATP)/(ADP) ratio significantly depended on the treatments given to the water. Out results indicated (1) that a reduction in exposure to EMFs increased NAD activity and (2) that electronic devices that had been exposed to a human intention concerned with energy metabolism, positively influenced both the activity of NAD and the ATP/ADP ratio in vitro. KEYWORDS: Human intention, Nicotinamide Adenine Dinucleotide (NAD), Adenosine Trisphosphate (ATP), Adenosine Diphosphate (ADP), electromagnetic fields, intention imprinted electronic device (lIED), Alzheimer's, AIDS Subtle Energies 6- Energy Medicine • VoLume 12 • Number 3 • Page 157 INTRODUCTION recent study has favorably implicated the coenzyme nicotinamide adenine dinucleotide (NADH) as a medication for patients suffering A from dementia of the Alzheimer type. 1 In all patients evaluated in that study, an improvement in their cognitive dysfunction was observed. In another study it was shown that HIV-I infection of human cells in vitro leads to significant decreases in the intracellular concentration of NAD.2 This decrease has been shown to vary with viral load and HIV strain. They conclude from their study that HIV induces a state of intracellular pellagra, which is reversed by the administration of nicotinamide which, in turn, maintains increased intracellular NAD concentration in HIV infected cells. 2 Very recently, we have shown that it is possible to influence the thermody­ namic activity of NAD and the election transport chain to increase the (ATP)/(ADP) ratio in developing fruit fly larvae and thus, significantly increase the fitness of these larvae. 3,4 In addition, using the same basic technique, the thermodynamic activity of the liver enzyme alkaline phosphatase (ALP) has been significantly increased.3-5 Because of the potential applicability of this new technique to AIDS and Alzheimers diseases, a study to be reported on here has been undertaken with the in vitro fly model system. Based on the present paradigm, the conventional viewpoint is that humans cannot meaningfully interact, via their intention, with specific target experi­ ments. Even more strongly, one would state that human intention cannot possibly be captured in a simple electronic device and then have the device meaningfully interact with these target experiments. Over the past two years, three specific and very different target experiments have been conducted using intention imprinted electronic devices (lIED's) and found robust interaction between these simple devices and the target experiments in complete opposi­ tion to the prevailing paradigm.3-8 On the experimental side, for each target experiment, one starts with two identical physical devices, isolates one from the other to be the control and "charges" one with the specific intention for the particular experiment. This device is then named an Intention Imprinted Electronic Device (lIED) and is described in detail in reference 3. This charging process involved the services Subtle Energies & Energy Medicine • Volume 12 • Number 3 • Page 158 of four meditators to impart the intention (imprint) to the device with the specific intention. The devices with the same imprint were then wrapped in aluminum foil and stored in electrically grounded Faraday cages until the next step in the process. The Faraday cage blocks electromagnetic radiation of wavelength greater than the spacing of the wire mesh used in the cage construc­ tion. The aluminum foil blocks electromagnetic radiation of even shorter wavelength, including photons. Next, when needed, the aluminum foil wrapped devices were separately shipped on different days, via Federal Express, to their laboratory destination about 2,000 miles away. On arriving there, they were immediately placed in separate, electrically grounded Faraday cages until use in the actual target experiment conducted by others (see Figure 1). For the specific target experiments, the general intentions were (1) to decrease (increase) the pH of water by one full pH unit, (2) to increase the (ATP)/(ADP) ratio in fruit fly larvae so as to significantly decrease the development time and (3) to significantly increase the thermodynamic activity of the liver enzyme ALp'3-8 The strong success of these three experimental studies promises to have important consequences for future medicine. The present paper continues the vector of progress for this new technique. In this paper we show that these electronic devices significantly alter the condition of the water used for preparing NAD solutions to be utilized for in vitro fruit fly studies. Here, both NAD activity and the (ATP)/(ADP) ratio are shown to significantly depend on the four treatments given to the water. EXPERIMENTAL METHODS TREATMENTS he study involved three simultaneous experimental variants with respect to the control exposure (C) with all four placed adjacent to each other T in an incubator at 4°C. The three variants were (1) an identical exposure in a 30cm diameter Faraday Cage with no device present (F), (2) the same as (1) but with an un imprinted device (IIED) at cage center present (d,o) and (3) the same as (1) but with an imprinted device at cage center present (d,}). The Faraday cage represents a shielded environment which facilitates Subtle Energies Energy Medicine - Volume 12 -Number 3 - Page 159 exposure to both fewer and specific EMFs (see above). The experimental layout is shown in Figure 1. In our experiments, comparisons between (d,o) and (d,}) indicate the effects of exposure to the two different electronic devices--the intention effect. Comparisons between (C) and (F) indicate the effect of a reduction in exposure to random environmental EMFs--the Faraday cage effect. Comparisons between (F) and (d,o) and (d,}) indicate the effects of the addition of specific EMFs to the Faraday cage environment-the oscillator effect. ELECTRONIC DEVICES ur experiments utilized a triple oscillator electronic device producing frequencies of 5.0 MHz, 8.0 MHz and 9.3 MHz. The devices were O powered by line voltage to 9V DC. A detailed description of the devices, including circuit diagrams, is given in references 3 and 5. We studied two categories of EMFs produced by these devices. The first category involved devices (d,o) which had not been exposed to human informational influences. The second category involved lIED devices (d,]) which had been exposed to human informational influences (see below). Thus, (d,o) and (d,}) constituted physically identical pairs of devices which differed only in the fact that one of the pair, (d,o) and (d,}) respectively, had been exposed to the human informa­ tional influence. The devices were individually wrapped in aluminum foil and stored in separate Faraday cages and were fabricated to be identical to those produced commercially.9 INTENTION The actual imprinting procedure was as follows: (i) place the device along with its current transformer on a table around which the imprinters sit, (ii) four people (two men plus two women) who were all accomplished meditators, coherent, inner-self managed and readily capable of entering an ordered mode of heart function and sustaining it for an extended period of time, sat around the table ready to enter a deep meditative state, (iii) a signal was then given to enter such an internal state, to cleanse the environment and create a sacred space for the intention.3,lO Then, a signal was given by one of the four to put attention on the table-top objects and begin a mental cleansing process to erase Subtle Energies & Energy Medicine • Volume 12 • Number 3 • Page 160 Faraday Cage Devi\ , NAD d,O roo I I 0 0 I I 0 0 I ~~~ C Figure 1a. Experimental layout. Meditators impart No intention an intention to the device (Imprinted) Devices are individually wrapped in aluminum foil and sent to the laboratory for the target experiments \ I Treatmen1s NAD r-,,-I-, !DO-'~D~-~ Icc I I I I 0 0 I 1.. ___ -1 ~~U (C) (d.n (do) (F) liED Figure lb. Flow diagram showing the creation of the lIED and the target experiments. Subtle Energies & Energy Medicine Volume 12 • Number 3 • Page 161 any prior imprints from the device, (iv) after 3 or 4 minutes, another signal was given to begin focussing on the specific prearranged intention statement for about 10-15 minutes, (v) next, a final signal was given to shift focus to a closing intention designed to seal off the imprint projected into the device and minimize the leakage of the essential energy/information from the devices. This completed the process so the four people withdrew from the meditative state and returned to their normal state of consciousness. The specific intention, here, was "to activate the indwelling consciousness of the device (d,j) so as to increase the concentration of NAD plus the activity of the available enzymes, dehydrogenases and ATP synthase in the mitochon­ dria so that production of ATP is significantly increased relative to that produced in the unimprinted device (d,o)." EXPERIMENTS (NAD) Change Over Time e initially exposed purified water (ASTM Type 1) to the treatments (d,o), (d,j) , (F) and (C) for 2 weeks.? We named these treated W waters W.o, w.j, W,F and W,C.
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