ATHYMIC Nude Immundeficient Mouse Detailed Specifications

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ATHYMIC Nude Immundeficient Mouse Detailed Specifications

ATHYMIC Nude Immundeficient Mouse Origin: Bred in JANVIER LABS since 2016 Strain name: Rj:ATHYM-Foxn1nu/nu Type : Mutant mouse Colour and related genotype: homozygous nu/nu, phenotype without fur “nude” Detailed specifications Main application and research fields Oncology Transplantation: xenograft and allograft Experimental infections Monoclonal antibodies: study Immunology Description of our model The athymic nude mouse is a laboratory mouse originating from a strain presenting a natural genetic mutation causing a total or partial absence of the thymus, a characteristic accompanied by a deficient immune system due to a reduced number of produced T lymphocytes. The three main defects of the homozygous mice for the spontanous mutation “nude” are an abnormal growth of hair, a defective development of the thymic epithelium and a lack of immunity. Even though the mice seem hairless, they are born with follicles for the growth of hair partially defective. The growth cycles and models of the hair are evident, in particular in case of pigmented mice, but the defective follicles don’t allow the hair to grow correctly during the entire cycle. The phenotype of the athymic nude model is characterized by the absence of hair, which gave it the “nude” name. The gene responsible for the mutation has been categorised as being a member of the Fox gene family and the nomenclature recommends the denomination Foxn1nu/nu. The athymic nude mouse is obtained by fixing the gene Foxn1nu/nu on a genetic BALB/cAnNRj background and by proceeding with the breeding of this new strain in outbreeding mode. The animal has either no thymus (total absence), or, if it is present, it is generally partial and not functional. The mouse is unable to produce T lymphocytes and is therefore immunodeficient. It suffers from a lack of cell-mediated immunity: the nude homozygous mice presents a partial defect in the development of B cells. Because of a defect in the activity of lymphocytes T helper, the response to thymus-dependent antigens are, when detectable, mostly limited to lgM. The athymic nude mouse is precious for research, because it can receive different tissue or tumour transplants without rejecting them. These xenografts are often used in research to test new methods of imaging or anti-tumour treatments. CHARACTERISTICS The nude homozygous females are not efficient breeders. The ovulation starts late at 2,5 months and ends early at 4 months. Hairless (no fur), albino background Bred in outbreeding mode. Health monitoring reports .

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Contribution of T Cell-Mediated Immunity to the Resistance
0022-202X/ 78/ 7006 0345$02.00/ 0 THE JOURNAL OF I NVESTIGATIVE DERMATOLOGY, 70:345-347, 1978 Vol. 70, No. 6 Copyright © 1978 by The Williams & Wilkins Co. Printed in U.S.A. Contribution ofT Cell-mediated Immunity to the Resistance to Staphylococcal Infection SHINGO TSUDA, M.D., YOICHIRO SASAI, M.D., KIKUO MINAMI, M.D., AND KIKUO NOMOTO, M.D. Department of Dermatology, Kurume University School of Medicine, Kurume, Japan, and Department of Immunology, Institute for Cancer Research, Kyushu University School of Medicine, Fuhuoha, Japan. Abscess formation in nude mice after subcutaneous -, DNase + and hemolysis of a type. The bacteria were cultured in a inoculation of Staphylococcus aureus (S. aureus) was meat infusion broth (distilled water 100 ml, meat extract obtained from more extensive and prolonged as compared with that in beef 5 gm, NaCl5 gm and NaHC03 0.5 gm) at 37°C for 24 hr, centrifuged phenotypically normallittermates. Abscess formation in at 3000 rpm for 30 min and resuspended in saline. Bacterial suspensions comparable to 1.7 x 107 colony-forming markedly by whole-body ir were adjusted to a dose nude mice was augmented units/ ml after estimation with a spectrophotometer at 580 nm (Hita radiation. Not only T cell-mediated immunity but also chi). radiosensitive, nonimmune phagocytosis appear to con tribute to the resistance against staphylococcal infec Procedures for Experimental Infection tion. Each mouse was inoculated subcutaneously with 0.1 ml of bacterial suspension into the back and the infected site was examined macro scopically 1, 2, 3, 5, 7, 10, 14, 21 and 27 days later.
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