nutrients Article Transcriptomic Analysis of LNCaP Tumor Xenograft to Elucidate the Components and Mechanisms Contributed by Tumor Environment as Targets for Dietary Prostate Cancer Prevention Studies Lu Yu 1, Robert W. Li 2 , Haiqiu Huang 1 , Quynhchi Pham 3, Liangli Yu 1 and Thomas T. Y. Wang 3,* 1 Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA;
[email protected] (L.Y.);
[email protected] (H.H.);
[email protected] (L.Y.) 2 Animal Parasitic Diseases Laboratory, Beltsville Area Research Center, ARS, USDA, Beltsville, MD 20705, USA;
[email protected] 3 Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, ARS, USDA, Beltsville, MD 20705, USA;
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[email protected]; Tel.: +1-(301)-504-8459 Abstract: LNCaP athymic xenograft model has been widely used to allow researchers to examine the effects and mechanisms of experimental treatments such as diet and diet-derived cancer preventive and therapeutic compounds on prostate cancer. However, the biological characteristics of human LNCaP cells before/after implanting in athymic mouse and its relevance to clinical human prostate outcomes remain unclear and may dictate interpretation of biological efficacies/mechanisms of Citation: Yu, L.; Li, R.W.; Huang, H.; diet/diet-derived experimental treatments. In this study, transcriptome profiles and pathways of hu- Pham, Q.; Yu, L.; Wang, T.T.Y. man prostate LNCaP cells before (in vitro) and after (in vivo) implanting into xenograft mouse were Transcriptomic Analysis of LNCaP compared using RNA-sequencing technology (RNA-seq) followed by bioinformatic analysis. A shift Tumor Xenograft to Elucidate the from androgen-responsive to androgen nonresponsive status was observed when comparing LNCaP Components and Mechanisms xenograft tumor to culture cells.