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INFORMATION TO USERS This was produced from a copy of a document sent to us for microfilming. While the most advanced technological means to photograph and reproduce this document have been used, the quality is heavily dependent upon the quality of the material submitted. The following explanation of techniques is provided to help you understand markings or notations which may appear on this reproduction. 1. The sign or "target” for pages apparently lacking from the document photographed is "Missing Page(s)”. If it was possible to obtain the missing page(s) or section, they are spliced into the film along with adjacent pages. This may have necessitated cutting through an image and duplicating adjacent pages to assure you of complete continuity. 2. When an image on the film is obliterated with a round black mark it is an indication that the film inspector noticed either blurred copy because of movement during exposure, or duplicate copy. 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In all cases we have filmed the best available copy. University Microfilms International 300 N. ZEEB RD., ANN ARBOR, Ml 48106 8121836 N g , K w o kei Ja c o b GAS CHROMATOGRAPHIC-MASS SPECTROMETRIC PROFILING OF ORGANIC ACIDS OF HUMAN AMNIOTIC FLUID The Ohio Stale University PH.D. 1981 University Microfilms Internationai300 N. Zeeb Road, Ann Arbor, M I 48106 Copyright 1981 by Ng, Kwokei Jacob All Rights Reserved GAS CHROMATOGRAPHIC— MASS SPECTROMETRIC PROFILING OF ORGANIC ACIDS OF HUMAN AMNIOTIC FLUID DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University by Kwokei Jacob Ng •k ' k ' k & ' k The Ohio State University 1981 Approved by Reading Committee: Brian D. Andresen , ; ■ / // / Joseph R. Bianchine /r"~ A 1'- A I Richard H. Fertel Advisor Philip B. Hollander Department of Pharmacology College of Medicine ACKNOWLEDGMENTS Since the beginning of this project, the constant support, enthusiasm, advice and assistance in many ways from Dr. Bianchine and Dr. Andresen have enable me to accomplish the present work. It is also a great blessing and pleasure to have the opportunity to serve apprenticeship with Dr. Andresen. He has spent many long late hours teaching me patiently the fine arts of gas chromatography and mass spectrometry, showing me how to apply the scientific method for clinical assays. The design of the helium preheater would not have been completed without his expertise. I would like to thank members of the Reading Committee and Graduate School Representative, Dr. Burkman for their time in reading my dissertation and suggestions. I would also like to thank Drs. Zuspan, 0'Shaunghessy, lams, Rayburn, Freeman and Stemple for their enthusiasm and supply of amniotic fluid samples and other biological specimens. Special thanks are due to Dr. Fertel who has been very helpful in many ways. I would also like to thank Dr. Tejwani, Dr. Tjoe and Dr. Gerber for their interest in my research work. Mrs. Judy Lubbers has been very kind to share with me her capillary columns and experiences in handling them. The friendship of fellow graduate students is a cherisable memory. Lastly, but not the least, the love and care of my family and my wife, Shirley, have made the present effort the more worthwhile. ii VITA May 1, 1943......................... Born - Canton, China 1963................................ Northcote Teachers' Training College, Hong Kong 1963-1968.......................... School teacher, St. Louis School Hong Kong 1969-1973.......................... B.S., Department of Chemistry, California State University, Northridge 1973-1976........................... M.S., Departments of Biochemistry & Pharmacognosy, Columbus, Ohio State University 1977............................... Research Assistant I,II Department of Radiology, Columbus, Ohio 1978-1979........................... Research Associate, Department of Radiology, Columbus, Ohio State University 1980-1981........................... Ph. D., Department of Pharmacology, Columbus, Ohio State University PUBLICATIONS "Longevity, Stability and DNA Repair" by Hart, R.W., D'Ambrosio, S.M., Ng, K.J., and Modak, S.P. Mechanism of Ageing and Development, 9, 203 (1979). "Helium Preheater for the Open-split Interface" by B.D. Andresen, K.J. Ng, J. Wu and J.R. Bianchine. Biomedical Mass Spectrometry (in print) FIELDS OF STUDY Major Field: Pharmacology Others: Biochemistry. Drs. Serif, Barber, Gross, Behrman Ives, Royer Radioisotope Methodology. Drs. Malspeis and Feller Organic Chemistry. Drs. Newman, Paquette, Swenton Carbohydrate Chemistry. Dr. Horton Natural Product Isolation Techniques. Dr. Doskotch Medical Physiology. Drs. Grossie, Lipsky, Weiss, Dujardin Biochemical Methods. Drs. Ives, Behrman, Gross Methods in Organic Chemistry. Dr. Ouellette Gas Chromatography and Mass Spectrometry. Drs. Andresen, Hammar, Wong TABLE OF CONTENTS Page ACKNOWLEDGMENTS................... ii VITA........................................................ iii LIST OF TABLES.............................................. vii LIST OF FIGURES............................................. viii I. INTRODUCTION......................................... 1 A. Introduction..................................... 1 B. Funtions of Amniotic fluid....................... 2 C. The origin of amniotic fluid.............. 2 D. Amniocentesis................................... 5 E. Prenatal diagnosis by amniocentesis.............. 8 F. Components of amniotic fluids.................... 16 1. Cellular..................................... 16 2. Macromolecules............................... 16 3. Organic acids........................... 21 4. Organic bases................................ 44 5. Hormones..................................... 46 6. Drugs........................................ 48 G. GC-MS Methodology................................ 50 H. Objectives of present investigation.............. 56 II. Materials and Methods A. Biological samples............................... 57 v Page B. Extraction of amniotic f l u i d . ..... 58 C. Derivatization of acidic fractions...................... 62 D. Gas chromatography-mass spectrometry analysis of samples................................................. 65 III. Results A. Stabilization of baseline with open-split interface heater............................ 70 B. Cleanliness of instruments and purity of reagents........ 74 C. Capillary GC-MS profiles of acidic fractions of 1. amniotic fluid of early pregnancy................... 82 2. amniotic fluid of late pregnancy.................... 86 3. amniotic fluid of pregnancy with open tubular neural defects...................................... 88 4. amniotic fluid of pregnancies with Rh sensitization.. 89 5. amniotic fluid of pregnancy from smoking mother 92 6. amniotic fluid of pregnancy with Delalutin treatment.. 94 7. amniotic fluid of pregnancy with hydrops............ 96 8. amniotic fluid of diabetic pregnancy................ 97 9. amniotic fluid from pregnancy of Down syndrome and gastrointestinal block............................ 99 10. amniotic fluid from drug addicted mother............. 100 D. Capillary GC-MS profile of acidic fraction of serum...... 101 E. Capillary GC-MS profile of acidic fraction of ovarian cyst fluid........................................ 103 F. Comparison of organic acids identified in this study with those identified by GC-MS profiling of amniotic fluid in the literature.......................................... 105 IV. Conclusions................................................. 109 V. Bibliography...................... 115 VI. Appendices Appendix A. Compilation of capillary GC-MS profiles of basic fractions of amniotic fluid................... 124 Appendix B. Compilation of mass spectra................ 139 vi LIST OF TABLES Examples of genetic disorders and the corresponding diagnostic tests...................................... 9 Proteins, peptides and enzymes in amniotic fluid...... 20 Lists of organic acids in amniotic fluid identified by GC-MS profiling in literature...................... 30 Steroidal hormones and metabolites in amniotic fluid.. 46 Non-steroidal hormones in amniotic fluid............. 47 Drugs and exogenous chemicals detected in amniotic fluid.................. ................ .............. 48 List of capillary GC-MS analyzed samples and patient informations.......................................... 80 List of identified compounds with the corresponding peak numbers.......................................... 81 Comparison of organic acids identified in the literature with those of current
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  • ALEX® Allergen List (Extracts and Components)

    ALEX® Allergen List (Extracts and Components)

    ALEX® allergen list (extracts and components) POLLEN w211 Wall pellitory rPar j 2 nsLTP 6 w10 White goosefoot TREE POLLEN w100 White goosefoot rChe a 1 Ole e 1-family 2 Code Allergen Source Component Protein Name t19 Acacia MOULD AND YEAST FUNGI t100 Alder Aln g 1 PR-10 protein 1 m229 Alternaria alternata rAlt a 1 Alt a 1-family t101 Alder Aln g 4 Polcalcin 4 t226 Arizona cypress nCup a 1 Pectate lyase 5 m230 Alternaria alternata rAlt a 6 Enolase t25 Ash m218 Aspergillus fumigatus rAsp f 1 Mitogillin Family 2 Peroxysomales t103 Ash rFra e 1 Ole e 1-family m220 Aspergillus fumigatus rAsp f 3 t300 Beech rFag s 1 PR-10 protein 1 protein t215 Birch rBet v 1 PR-10 protein 1 m221 Aspergillus fumigatus rAsp f 4 unknown Mn superoxide t216 Birch rBet v 2 Profilin m222 Aspergillus fumigatus rAsp f 6 t225 Birch rBet v 6 Isoflavon dismutase m2 Cladosporium her- reductase t222 Cypress barum t105 Date tree nPho d 2 Profilin 3 m100 Cladosporium her- rCla h 8 Short-chain dehy- t8 Elm barum drogenase y2 Malassezia sympo- rMala s 5 unknown t4 Hazel t102 Hazel rCor a 1.0103 PR-10 protein 1 dialis t303 Sugi rCry j 1 Pectate Lyase y3 Malassezia sympo- rMala s 6 Cyclophilin t6 Mountain ceder dialis t71 Mulberry y5 Malassezia sympo- rMala s 11 Mn superoxide t224 Olive nOle e 1 Ole e 1-family 2 dialis dismutase t240 Olive rOle e 9 1,3 β Glucanase m1 Penicilium chrysoge- t305 Paper mulberry num t241 Plane tree rPla a 1 Plant invertase MITES AND COCKROACHES t301 Plane tree nPla a 2 Polygalacturonase d70 Acarus siro t302 Plane tree rPla a 3 nsLTP 6 i100 Blatella