ALEX (suchasmelon) - Rarely associated withclinical symptoms, oral allergy syndrome possible - Sensitive to heatanddigestion - Cross-reactive, pan-allergen inallpollen andvegetarian foods Profilins - Associated withallergic rhinitis Marker - for sensitisationsregarding olives, ashtree, lilac,andprivet pollen Ole e1family hazelnut,apple) - Associated withallergic rhinitisandoral allergy syndrome (suchas - Usually sensitive to heatanddigestion - Cross-reactive found invegetarian foods (pipandstone fruits, tree nuts, vegetables, legumes) Most important tree - pollen allergen withinthebeechfamily, can alsobe PR-10 proteins (Betv1homologues) INFORMATION REGARDING THEMOSTIMPORTANT ALLERGENFAMILIES t302 t301 t241 t305 t240 t224 t71 t6 t303 t102 t4 t8 t105 t222 t225 t216 t215 t300 t103 t25 t226 t101 t100 t19 Code TREE POLLEN POLLEN w21 w232 w11 w20 w18 w300 w230 w1 w233 w231 w6 w302 w303 w301 w234 w9 w14 WEED POLLEN g212 g210 g209 g215 g206 g205 g100 g12 g7 g216 g2 g17 GRASS POLLEN t10 t304 t14 ® allergen list (extracts and 3 2 Wall pellitory Russian thistle Russian thistle Stinging nettle Sorrel Ragweed Ragweed Ragweed Mugwort Mugwort Mugwort Hemp Hemp (CBD) Herb mercury Buckhorn Buckhorn Amarant Timothy grass Timothy grass Timothy grass Timothy grass Timothy grass Timothy grass Rye grass Rye Reed Bermuda grass Bermuda grass Bahia grass Walnut Tree ofheaven Poplar Plane tree Plane tree Plane tree Paper mulberry Olive Olive Mulberry Mountain ceder Sugi Hazel Hazel Elm Date tree Cypress Birch Birch Birch Beech Ash Ash Arizona cypress Alder Alder Acacia Allergen Source 1 rFra e1 nCup a1 Aln g4 Aln g1 Component rMer a1 rPla l1 rPhl p12 rPhl p7 rPhl p6 rPhl p5.0101 rPhl p2 rPhl p1 nLol p1 rCyn d1 rPla a3 nPla a2 rPla a1 rOle e9 nOle e1 j1 rCry nPho d2 rBet v6 rBet v2 rBet v1 rFag s1 rSal k1 rSal rAmb a4 rAmb a1 v3.0201 rArt v1.0101 rArt rCan s3 rCor a1.0103 components Ole e1-family Pectate lyase Polcalcin PR-10 protein Protein Name Profilin Ole e1-family Profilin Polcalcin Grass group 5/6 Grass group 5/6 Expansin Beta-expansin Beta-expansin Beta-expansin nsLTP Polygalacturonase Plant invertase 1,3 Ole e1-family Pectate Lyase Profilin reductase Isoflavon Profilin PR-10 protein PR-10 protein esterase Pectin Methyl- Plantdefensin Pectate lyase nsLTP Plant’s defensin nsLTP PR-10 protein ) β Glucanase 6 6 6 3 3 3 4 4

5 5 2 2 2 1 1 1 1

- Prevalence comparably low innorthernandmiddle Europe (suchasgrapes, blueberries) - Associated withbothoral allergy syndrome andsystemic reactions - Stable regarding heatanddigestion - Pan-allergen inpollen andvegetarian foods Non-specific lipidtransfer proteins (nsLTP) - Cross-reactive withinthecypress family - Marker allergen for cypress allergy (prevalence 100%) Pectate lyase (Cupa1) Cross-reactive, - pan-allergen inallpollen, however notinvegetarian foods Polcalcins d300 i301 i103 i102 i101 i100 d70 MITES ANDCOCKROACHES m1 y5 y3 y2 m100 m2 m222 m221 m220 m218 m230 m229 MOULD ANDYEASTFUNGI w100 w10 w211 d105 d209 d304 d303 d102 d205 d104 d103 d203 d202 d101 d100 d302 d301 4 Blomia tropicalis Blomia germanica Blatella germanica Blatella germanica Blatella germanica Blatella germanica Acarus siro num Penicilium chrysoge dialis Malassezia sympo dialis Malassezia sympo dialis Malassezia sympo barum Cladosporium her barum Cladosporium her Aspergillus fumigatus Aspergillus fumigatus Aspergillus fumigatus Aspergillus fumigatus Alternaria alternata Alternaria alternata White goosefoot White goosefoot Wall pellitory sticus Glycyphagus dome pteronyssinus Dermatophagoides pteronyssinus Dermatophagoides pteronyssinus Dermatophagoides pteronyssinus Dermatophagoides pteronyssinus Dermatophagoides pteronyssinus Dermatophagoides pteronyssinus Dermatophagoides pteronyssinus Dermatophagoides pteronyssinus Dermatophagoides farinae Dermatophagoides farinae Dermatophagoides Blomia tropicalis Blomia tropicalis 5 ------rBlo t5 rBla g9 rBla g5 rBla g4 rBla g2 rBla g1 rMala s11 rMala s6 rMala s5 rCla h8 rAsp f6 rAsp f4 rAsp f3 rAsp f1 rAlt a6 rAlt a1 rChe a1 rPar j2 rGly d2 rDer p23 rDer p21 rDer p20 rDer p11 rDer p10 rDer p7 rDer p5 rDer p2 rDer p1 rDer f2 rDer f1 rBlo t21 rBlo t10 6 Mite, group 5 Arginin Kinase transferase Glutathione S- Lipocalin Aspartate protease Cockroach group 1 dismutase Mn superoxide Cyclophilin unknown drogenase Short-chain dehy dismutase Mn superoxide unknown protein Peroxysomales Mitogillin Family Enolase Alt a1-family Ole e1-family nsLTP NPC2 family domain III, peritrophin Chitinase, class unknown Arginine Kinase chain Myosin, heavy Tropomyosin Mite group 7 unknown NPC2 family Cysteine protease NPC2 family Cysteine protease unknown Tropomysin 6 7

12 2

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Diag-Info: 318 / Seite 2 - 4 / Version: 4 - Cross-reactive (suchasbird-egg, orpig-cat-syndrome) - Sensitive to heatanddigestion - Food allergen inmilk,egg,meat - Minorinhalationallergen from animalfur Serumalbumins (suchascat, dog,andhorse) - Cross-reactivity limited, however possible across different animalspecies Associated withrespiratory - system symptoms andincreased riskofasthma - Stable air-born pollutants - Majorallergens inmammals Lipocalins INFORMATION REGARDING THEMOSTIMPORTANT ALLERGENFAMILIES ALEX e103 e307 e306 e227 e301 e305 e80 e304 e303 e308 e302 e221 e102 e101 e100 e300 e228 e220 e94 ANIMAL EPITHELIA d306 d72 i300 i206 d305 f352 f286 f300 f78 f77 f76 f2 f510 f75 k208 f323 f232 f233 f1 f506 EGG ANDMILK FOOD i210 i4 i25 i70 i217 i208 i1 i209 i211 i3 INSECT VENOM e81 e73 e311 e310 e309 e83 ® 7 allergen list (extracts and 8 Sheep’s milk Mare’s milk Goat’s milk Cow’s milk Cow’s milk Cow’s milk Cow’s milk Chicken’s eggyolk Chicken’s eggyolk Chicken’s eggwhite Chicken’s eggwhite Chicken’s eggwhite Chicken’s eggwhite Chicken’s eggwhite Camel’s milk Paper Wasp Paper Wasp Long-headed wasp Fire ant Honey Bee Honey Bee Honey Bee Common Wasp Common Wasp Common Wasp scentiae Tyrophagus putre scentiae Tyrophagus putre Periplaneta americana Periplaneta americana structor Lepidoglyphus de Dog Dog Can f5) Dog, male urine(incl. Dog Dog Dog Dog Cow Cat Cat Cat Cat Sheep Rat Rabbit Rabbit Rabbit Pig Mouse Horse Horse Horse Djungarian hamster Guinea Pig Goat - - - rTyr p2 rPer a7 rLep d2 nBos d8 nBos d5 nBos d4 nGal d5 nGal d4 nGal d3 nGal d2 nGal d1 rPol d5 rApi m10 n/r Apim1 rVes v5 rVes v1 rOry c3 rOry c2 rOry c1 rOry nMus m1 rEqu c4 rEqu c3 rEqu c1 rPhod s1 rCap c1 1 like rCan fFel d rCan f6 rCan f4 nCan f3 rCan f2 rCan f1 rBos d2 rFel d7 rFel d4 rFel d1 nFel d2 components NPC2 family Tropomyosin NPC2 family Casein b-Lactalbumin a-Lactalbumin Serum albumin Lysozyme C Ovotransferrin Ovalbumin Ovomucoid Antigen 5 Icarapin variant 2 Phospholipase A2 Antigen 5 Phospholipase A1 Uteroglobin Lipocalin Lipocalin Lipocalin Latherin Serum albumin Lipocalin Lipocalin Lipocalin Uteroglobin Lipocalin Lipocalin Serum albumin Lipocalin Lipocalin Lipocalin Lipocalin Lipocalin Serum albumin Uteroglobin ) 7 7 7 7 7 7 7 7 7 7 7 7 7 12

8 8 8 8 (WDEIA) - Inadults: riskmarker ofwheat-dependentexercise-induced anaphylaxis - Inchildren: marker ofwheatallergy Gliadins - Usually associated withsevere reactions (riskofanaphylaxis) - Stable regarding heatanddigestion Storage Proteins (2S albumins,11Sglobulins, 7/8globulins) f80 f525 f205 f515 f805 f509 f3 f23 f355 f207 f37 f529 f524 f552 f545 f517 p11 p10 FISH ANDSEAFOOD f284 f213 f530 f26 f553 f527 f88 f526 f321 f83 e204 f27 MEAT f347 f516 f224 f226 f7 f55 f335 f305 f523 f8 f508 f11 f6 CEREALS ANDSEEDS f538 f40 f536 f535 f537 f258 f24 f534 f41 f338 f290 f551 f206 10 Lobster Herring Herring Coldwater prawn Cod Cod Cod Crab Carp Clam Blue mussel Brown Shrimp Black TigerShrimp Black TigerShrimp Black TigerShrimp Black TigerShrimp Anisakis simplex Anisakis simplex Turkey Rabbit Pork Pork Migratory locust Mealworm Lamb House cricket Horse Chicken Beef Beef Quinoa Poppy seed Poppy seed Pumpkin seed Oat Millet Lupin seed Fenugreek seeds Corn, grains Corn, grains Buckwheat Buckwheat Barley Tuna Tuna Thornback ray Thornback ray Swordfish Squid Shrimp mix Salmon Salmon Scallop Oyster Mackerel Mackerel rSus d1 rSus nBos d6 rClu h1 nGad m2&3 nGad m1 rCyp c1 c6 rCra rPen m4 nPen m3 nPen m2 nPen m1 rAni s3 rAni s1 Albumin nPap s2S rZea m14 nFag e2 Thu a1 albumin rRaj cParv rXip g1 s1 rSal rSco s1 - Serum albumin Serum albumin β Aldolase β β β Troponin c Protein Calcium Binding Sarcoplasmic Myosin lightchain Arginine Kinase Tropomyosin Tropomyosin protease inhibitor Kunitz serine 2S albumin nsLTP 2S albumin β α β β β -Parvalbumin -Enolase & -Parvalbumin -Parvalbumin -Parvalbumin -Parvalbumin -Parvalbumin -Parvalbumin -Parvalbumin 9 6 9 9

12 12 11 11 11 11 11 11 11 8 8

Diag-Info: 318 / Seite 3 - 4 / Version: 4 ALEX - Cross-reactive, pan-allergen inseafood, mites, cockroaches, andparasites - Proteins withhighallergenic potential thatare stable to heatanddigestion Tropomyosins - Cross-reactive, pan-allergen infish pollutants andthusbothfood andinhalation allergen - Proteins thatare stable to heatanddigestion, characteristics ofair-born Parvalbumins INFORMATION REGARDING THEMOSTIMPORTANT ALLERGENFAMILIES f315 f309 f443 f550 f202 f354 f18 f20 LEGUMES ANDNUTS f86 f218 f283 f519 f89 f265 f271 SPICES f544 f543 f542 k84 f124 f518 f10 f5 f9 f541 f540 f539 f512 f441 f511 f432 f431 f353 f533 f532 f531 f201 f803 f427 f352 f447 f424 f423 f422 f12 f513 f345 f235 f439 f522 f440 f425 f428 ® allergen list (extracts and Walnut Walnut Walnut Walnut Walnut Soy bean Soy bean Soy bean Soy bean Pistachio Pistachio Pistachio Pecan Peanut Peanut Peanut Peanut Peanut Peanut Peanut Pea Macadamia Macadamia Lentil Hazelnut Hazelnut Hazelnut Hazelnut Hazelnut Green bean Chickpea Cashew nut Cashew nut Cashew nut Brazil nut Brazil nut Almond Parsley Paprika Oregano Mustard Mustard Caraway Anise Wheat Wheat Wheat Sunflower seeds Spelt Sesame Sesame Rye, grains Rice 11 12

nJug r1 nGly m8 nGly m6 rGly m5 rGly m4 rPis v3 rPis v2 rPis v1 h15 rAra h9 rAra h8 rAra h6 nAra h3 rAra h2 rAra h1 nAra nMac i nCor 14 nCor a11 nCor a9 rCor a8 rCor a1.0401 rAna o3 rAna o2 n Bere1 nSin a1 nJug r6 nJug r4 nJug r3 nJug r2 nTri aaA_TI rTri a19 rTri a14 i1 nSes components 2S albumin 2S albumin 11S globulin 7/8S globulin PR-10 protein 7/8S globulin subunit 11S globulin 2S albumin Oleosin nsLTP PR-10 protein 2S albumin 11S globulin 2S albumin 7/8S globulin 2S albumin 2S albumin 7/8S globulin 11S globulin nsLTP PR-10 protein 2S albumin 11S globulin 2S albumin 2S albumin 7/8S globulin 11S globulin nsLTP 7/8S globulin Alpha-Amylase Omega-5-Gliadin nsLTP 2S albumin Trypsin-Inhibitor ) 6 6 6 6 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 1 1 1

- Low clinical relevance (venom) - Cross-reactive, includedinpollen allergens, vegetarian foods andinsect - Sensitisationmarker regarding carbohydrate determinants Cross-reactive Carbohydrate Determinants (CCD) f91 f502 f501 f503 f500 f521 f328 f242 f288 f92 f435 f514 f434 FRUITS f520 f25 f35 f48 f47 f505 f504 f417 f507 f31 f96 VEGETABLES o214 CCD o100 k224 k221 k220 k218 k217 k215 k81 ADDITIONAL f45 OTHER f44 f94 f420 f293 f33 f528 Mango Kiwi Kiwi Kiwi Kiwi Grape Fig Cherry Blueberry Banana Apple Apple Apple Tomato Tomato Potato Onion Garlic Celery Celery Celery Carrot Carrot Avocado Hom sLactoferrin Pigeon tick Latex Latex Latex Latex Latex Latex Ficus benjamina Baker‘s yeast Strawberry Pear Peach Papaya Orange Melon rDau c1 nAct d10 nAct d5 nAct d2 nAct d1 nVit v1 rMal d3 rMal d2 rMal d1 l6 nSola rApi g6 rApi g2 rApi g1 rHom sLF rArg r1 r Hev b11 r Hev b8 r Hev b6.02 r Hev b5 r Hev b3 r Hev b1 a 3 Fra a1+Fra r Prup3 rCuc m2 13 PR-10 protein nsLTP nsLTP Kiwellin TLP Cystein protease nsLTP nsLTP TLP PR-10 protein nsLTP nsLTP nsLTP PR-10 protein CCD Lipocalin Class 1chitinase Profilin Pro-hevein unknown particle protein small rubber on factor Rubber elongati PR-10 protein nsLTP Profilin 13 6 6 6 6 6 6 6 6 3 1 1 1 1 + -

Diag-Info: 318 / Seite 4 - 4 / Version: 4