Manuscript Clean Version Click here to download Manuscript Manuscript_DatPhyPaper_v23_CleanVersion.docx 1 Phylogenetics of Datureae (Solanaceae), including description of the new genus Trompettia 2 and re-circumscription of the tribe 3 4 Julia Dupin1* and Stacey D. Smith1 5 6 1 Department of Ecology and Evolutionary Biology, University of Colorado Boulder, 1800 7 Colorado Ave, Boulder, CO 80309, USA. 8 * Correspondence author:
[email protected] 9 10 Running head: Phylogenetics of Datureae (Solanaceae) 11 12 Abstract 13 14 Datureae G. Don is a tribe in the Solanaceae known for its charismatic large-flowered 15 species (jimsonweeds and angel trumpets). The monophyly of the tribe is well established, but the 16 recent finding that a species previously described in Iochroma (also Solanaceae) belongs in 17 Datureae calls for a reassessment of the tribe’s circumscription. Here we estimated the phylogeny of 18 Datureae, including of all of its 18 species, using three nuclear regions, and incorporated fossil 19 information to estimate divergence times. Based on this phylogeny, we reconstructed the evolution 20 of key aspects of reproductive morphology and life history to identify diagnostic features. Our 21 molecular phylogenetic analyses suggest that the diversification of Datureae began roughly ca. 35 22 Ma, around the beginning of the Andean uplift. Within the tribe, Datura and Brugmansia are 23 monophyletic sister taxa and the misplaced species of Iochroma is sister to the remaining species. 24 Based on our morphological analysis, we describe the latter as a new monotypic genus Trompettia. 25 Ancestral state reconstructions identify diagnostic features for each of the three genera and show a 26 large suite of changes along the Datura branch, including the evolution of erect flowers, capsular 27 fruit and annual life history.