DOCSLIB.ORG

Three New Species of Acanthostigma (Tubeufiaceae, Dothideomycetes) from Great Smoky Mountains National Park

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Three New Species of Acanthostigma (Tubeufiaceae, Dothideomycetes) from Great Smoky Mountains National Park Mycologia, 102(3), 2010, pp. 000–000. DOI: 10.3852/09-051 # 2010 by The Mycological Society of America, Lawrence, KS 66044-8897 Three new species of Acanthostigma (Tubeufiaceae, Dothideomycetes) from Great Smoky Mountains National Park Itthayakorn Promputtha cellular pseudoparaphyses, cylindrical to clavate, Andrew N. Miller1 bitunicate asci, and cylindrical-fusiform to elongate- University of Illinois, Illinois Natural History Survey, fusiform, hyaline, transversely multiseptate ascospores 1816 South Oak Street, Champaign, Illinois 61820- (Re´blova and Barr 2000). Members of Acanthostigma 6970 usually occur as saprobes on terrestrial decomposing wood (Rossman 1987, Re´blova and Barr 2000) but also have been found on submerged wood in Abstract: Three new bitunicate ascomycetes belong- freshwater (Kodsueb et al. 2006). Some species are ing to genus Acanthostigma are described from associated with helicosporous hyphomycete ana- terrestrial decomposing wood collected from Great morphs (Barr 1980, Re´blova and Barr 2000, Kodsueb Smoky Mountains National Park, USA. Phylogenetic et al. 2004, Tsui et al. 2006). analyses of the nuclear ribosomal 28S large subunit During our ongoing study of fungal diversity in and internal transcribed spacer region placed all three Great Smoky Mountains National Park (GSMNP) five species in the Tubeufiaceae and confirmed morpho- species of Acanthostigma were found, three of which logical analyses that these are distinct species. Expand- do not fit the description of any known species. These ed phylogenetic analyses of 28S large subunit includ- three newly discovered species are described, illus- ing taxa throughout the Dothideomycetes confirmed trated and compared morphologically and genetically the placement of Acanthostigma in the Tubeufiaceae. to other known species in the genus. A key to nine Acanthostigma filiforme differs from other Acanthos- accepted species in Acanthostigma is provided. tigma species in having longer ascospores with more septa. Acanthostigma multiseptatum can be distin- guished in having longer asci and longer ascospores MATERIALS AND METHODS with more septa. Acanthostigma septoconstrictum differs Morphological characterization.—Eighty-six collections of in having longer setae and asci and broader, asymmet- Tubeufiaceae were made 2004–2007, but morphological rical ascospores that are constricted at their septa. A examination occurred mostly throughout 2008. Due to the dichotomous key to Acanthostigma species is provided. large number of specimens collected during our inventory Key words: Ascomycota, ITS, LSU, saprobe, and because of the delayed morphological analyses cultures southern Appalachians, systematics, Tubeufia were not obtained from these specimens. Thus anamorphic data from cultures are not available for these species. Ascomata were squash-mounted in water and images of INTRODUCTION micromorphological structures were captured with a QIma- The taxonomic status of Acanthostigma de Not. (de ging QColor 3 digital camera mounted on either a Leica Notaris 1863) has had an obscure history mostly due MZ7.5 dissecting microscope with a Schott KL1500 fiber to the perceived loss of the type specimen for its type optics light source or an Olympus BX51 compound microscope with differential interference microscopy. Im- species, A. perpusillum de Not. The recent rediscovery ages were processed with Adobe Photoshop 7.0 (Adobe and re-examination of the type specimen of A. Systems Inc., Mountain View, California). A minimum of 30 perpusillum (Re´blova and Barr 2000) along with measurements was taken for all morphological structures molecular analyses including the type species (Tsui when possible with NIH Image 1.63 (National Institute of et al. 2006, 2007) has clarified the circumscription of Health, Bethesda, Maryland). Means, which are shown in Acanthostigma and confirmed its taxonomic place- brackets, were calculated for asci and ascospores. Ascomata ment in the Tubeufiaceae. The taxonomy of were sectioned at ca. 5 mm with a modified procedure of Acanthostigma has been discussed adequately else- Huhndorf (1991). Taxa were compared with published where (Mu¨ller 1965; von Arx and Mu¨ller 1975; Barr species descriptions of Acanthostigma taxa listed on Index 1977, 1980, 1990; Rossman 1987; Erikkson and Fungorum (www.indexfungorum.org, 1 Feb 2009). Hawksworth 1998; Re´blova and Barr 2000; Kodsueb Molecular sequencing.—A DNeasyH Mini Plant Extraction et al. 2006). Acanthostigma is characterized by having Kit (QIAGEN Inc., Valencia, California) was used to extract minute, dark, setose ascomata with thick-walled setae, DNA from dried ascomata following the manufacturer’s protocols, except tissues were not ground in liquid nitrogen Submitted 2 Apr 2009; accepted for publication 6 Oct 2009. and final elution volume was 25 mL. Ascomata initially were 1 Corresponding author. E-mail: [email protected] rehydrated in 50 mL AP1 buffer 3–5 h, followed by freezing 0 Mycologia myco-102-03-08.3d 23/12/09 20:23:26 1 Cust # 09-051R 0MYCOLOGIA at 280 C for 3–14 d before DNA extraction. Due to their respectively in the expanded LSU, limited LSU and ITS relatively small size 30–50 ascomata were used in the datasets. A portion of the phylogenetic signal was recovered extraction of DNA. The relative quantity of total genomic from all four regions in the limited LSU dataset and three of DNA was observed on a 1% TBE agarose gel stained with the nine regions in the ITS dataset by recoding them with the ethidium bromide. The entire internal transcribed spacer program INAASE (Lutzoni et al. 2000). The remaining six (ITS) region and the first 600 bp of the 59 end of 28S large ambiguously aligned regions in the ITS dataset and all 13 subunit (LSU) was PCR amplified on a Bio-Rad PTC 200 regions in the expanded LSU could not be recoded due to thermal cycler with PuReTaq Ready-To-GoTM PCR Beads their size so they were excluded from all analyses. The (GE Healthcare, Piscataway, New Jersey) and primers remaining unambiguously aligned characters in each dataset ITS1F/ITS5 and ITS4 or LROR and LR6 respectively were subjected to a symmetrical step-matrix to differentially (Vilgalys and Hester 1990, White et al. 1990, Gardes and weight nucleotide transformations with STMatrix 2.2 (Fran- Bruns 1993, Rehner and Samuels 1995). These reagents c¸ois Lutzoni and Stefan Zoller, Duke University), which were added to each PCR reaction: 1–5 mL DNA, 1.0 mL each calculates the costs for changes among character states based 10 mM primer and sufficient amount of sterile H20 to bring on the negative natural logarithm of the percentages of the total volume to 25 mL. Occasionally 2.5 mL BSA (bovine reciprocal changes between any two character states. serum albumin, New England Biolabs, Ipswich, Massachu- Unequally weighted MP analyses were performed with 1000 setts) and/or 2.5 mL DMSO (dimethyl sulfoxide, Fisher stepwise random addition heuristic searches, TBR branch- Scientific, Pittsburgh, Pennsylvania) also were added to the swapping, MULTREES option in effect, zero-length branches PCR reaction. These thermo-cycling parameters were used: collapsed, constant characters excluded and gaps treated as initial denaturation at 95 C for 5 min, followed by 35 or 40 missing. Branch support was estimated by performing 100 cycles of 95 C for 30 s, 41 or 50 C for 15 s, and 72 C for 1 min bootstrap replicates (Felsenstein 1985) each consisting of 10 with a final extension step of 72 C for 10 min. After stepwise random addition heuristic searches as above. verification on an ethidium bromide-stained 1% TBE Modeltest 3.7 (Posada and Crandall 1998) was used to agarose gel, PCR products were purified with either a determine the best-fit model of evolution. The model of QIAquick PCR purification kit (QIAGEN Inc.) or ExoSAP- evolution selected by Modeltest for the LSU datasets was the ITH (Affymetrix, Cleveland, Ohio) following the manufac- GTR model (Rodrı´guez et al. 1990), while the model selected turer’s recommendations. A BigDyeH Terminator 3.1 cycle for the ITS dataset was the TrN model (Tamura and Nei sequencing kit (Applied Biosystems Inc., Foster City, 1993). Both models included a proportion of invariable sites California) was used to sequence both strands with a with the remaining sites subjected to a gamma distribution combination of these primers: ITS 5 ITS1F, ITS5, ITS1, shape parameter. ML analyses were performed on the limited ITS4; LSU 5 LROR, LRFF1, LRAM1, LR3, LR3R, LR5, LR6 LSU and ITS datasets with the above models with 100 (Vilgalys and Hester 1990, White et al. 1990, Gardes and stepwise random addition replicates and TBR branch-swap- Bruns 1993, Rehner and Samuels 1995, Huhndorf et al. ping with a reconnection limit of 12. Constant characters 2004). Sequences were generated on an Applied Biosystems were included and ambiguously aligned characters were 3730XL high-throughput capillary sequencer. Each se- excluded from the ML analyses. Bayesian analyses were quence fragment was subjected to an individual BLAST performed on all datasets with MrBayes 3.1 (Huelsenbeck analysis to verify its identity. Sequences were assembled with and Ronquist 2001, Ronquist and Huelsenbeck 2003) as an Sequencher 4.7 (Gene Codes Corp., Ann Arbor, Michigan). additional means of assessing branch support. Constant characters were included, the above models of evolution Phylogenetic analyses.—Three datasets were assembled and were implemented and 50 000 000 or 100 000 000 genera- analyzed: (i) an expanded LSU dataset consisting of nearly all tions were sampled every 1000th generation resulting Dothideomycetes taxa included in Schoch et al. (2006) along respectively in 50 000 or 100 000 total trees for the ITS with representatives of Acanthostigma; (ii) a limited LSU and limited LSU datasets and expanded LSU dataset. The dataset consisting of previously published anamorphic and Markov chain always achieved stationarity after the first teleomorphic taxa in the Tubeufiaceae along with the newly 100 000 generations, so the first 10 000 trees, which extended sequenced taxa generated during this study and (iii) an ITS well beyond the burn-in phase of each analysis, were dataset with the same taxon sampling strategy used in the discarded.
Load more

Recommended publications
  • Phaeoseptaceae, Pleosporales) from China
    Mycosphere 10(1): 757–775 (2019) www.mycosphere.org ISSN 2077 7019 Article Doi 10.5943/mycosphere/10/1/17 Morphological and phylogenetic studies of Pleopunctum gen. nov. (Phaeoseptaceae, Pleosporales) from China Liu NG1,2,3,4,5, Hyde KD4,5, Bhat DJ6, Jumpathong J3 and Liu JK1*,2 1 School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, P.R. China 2 Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang 550006, P.R. China 3 Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand 4 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand 5 Mushroom Research Foundation, Chiang Rai 57100, Thailand 6 No. 128/1-J, Azad Housing Society, Curca, P.O., Goa Velha 403108, India Liu NG, Hyde KD, Bhat DJ, Jumpathong J, Liu JK 2019 – Morphological and phylogenetic studies of Pleopunctum gen. nov. (Phaeoseptaceae, Pleosporales) from China. Mycosphere 10(1), 757–775, Doi 10.5943/mycosphere/10/1/17 Abstract A new hyphomycete genus, Pleopunctum, is introduced to accommodate two new species, P. ellipsoideum sp. nov. (type species) and P. pseudoellipsoideum sp. nov., collected from decaying wood in Guizhou Province, China. The genus is characterized by macronematous, mononematous conidiophores, monoblastic conidiogenous cells and muriform, oval to ellipsoidal conidia often with a hyaline, elliptical to globose basal cell. Phylogenetic analyses of combined LSU, SSU, ITS and TEF1α sequence data of 55 taxa were carried out to infer their phylogenetic relationships. The new taxa formed a well-supported subclade in the family Phaeoseptaceae and basal to Lignosphaeria and Thyridaria macrostomoides.
    [Show full text]
  • MYCOTAXON Vol
    MYCOTAXON Vol. XII, No. 1, pp. 137-167 October-December 1980 té ON THE FAMILY TUBEUFIACEAE (PLEOSPORALES) MARGARET E. BARR Department of BotanyUniversity of Massachusetts, Amherst, Massachusetts 01003 SUMMARY Ten genera are presently accepted in the family Tubeuf- iaceae. Those genera whose species are extralimital in distribution to temperate North America are considered briefly. Rebentischia and Tubeufia, with two and seven species respectively in temperate North America, are con­ sidered in more detail. Tubeufia is subdivided into the sections Tubeufia, Nectrioidea, Thaxteriella and Acantho- stigmina; new combinations are proposed for Tubeufia clintoniij T. pezizula, and T. scopula. INTRODUCTION The family Tubeufiaceae was erected recently (Barr, 1979) to accommodate a number of pleosporaceous fungi that are typically either hypersaprobic on other fungi or on substr­ ates previously colonized by other fungi or hyperparasitic on foliicolous fungi, or parasitic on scale insects, occas­ ionally parasitic on living leaves. The ovoid, globose, ellipsoid or cylindric ascomata of species in the family are soft and fleshy in consistency, range in pigmentation from none (hyaline) to yellowish, brownish or pinkish to dark vinaceous brown, but not red; surfaces may be smooth or ornamented by protruding cells, hyphal appendages, or setae. The bitunicate asci are clavate or cylindric and develop from the base of the locule in narrow cellular pseudopara­ physes. Ascospores are hyaline, yellowish, or light vinac­ eous brown, narrowly oblong or nearly ellipsoid, short to elongate fusoid, or cylindric, and one or more commonly several septate. The conidial states known for a number of the species are hyphomycetous; sympoduloconidia are typically helicosporous or staurosporous, but dictyosporous conidia are associated in some taxa.
    [Show full text]
  • Helicosporous Hyphomycetes from China
    Fungal Diversity Helicosporous hyphomycetes from China Guozhu Zhao1, Xingzhong Liu1 and Wenping Wu2 1Key Laboratory of Systematic Mycology & Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China 2Novozymes China, Shangdi Zone, Haidian District, Beijing 100085, PR China Zhao, G.Z., Liu, X.Z, and Wu, W.P. (2007). Helicosporous hyphomycetes from China. Fungal Diversity 26: 313-524. Morphological studies of anamorphic taxa with helicospores (helicosporous fungi) were carried out based on observation of specimens collected in China and comparisons with descriptions in the literature. After examination of more than 300 freshly collected specimens and 100 herbarium specimens, we conclude that 71 species in 14 genera are presently known in mainland China, including 9 new species and 2 new combinations. The new species are Helicomyces denticulatus G.Z. Zhao, Xing Z. Liu & W.P. Wu; Helicosporium dentophorum G.Z. Zhao, Xing Z. Liu & W.P. Wu; Helicosporium sympodiophorum G.Z. Zhao, Xing Z. Liu & W.P. Wu; Helicoma hainanense G.Z. Zhao, Xing Z. Liu & W.P. Wu; Helicoma hyalonema G.Z. Zhao, Xing Z. Liu & W.P. Wu; Helicoma latifilum G.Z. Zhao, Xing Z. Liu & W.P. Wu; Helicoma scarabaeiforme G.Z. Zhao; Xenosporium latisporum G.Z. Zhao, Xing Z. Liu & W.P. Wu; Xenosporium ovatum G.Z. Zhao, Xing Z. Liu & W.P. Wu. The new combinations are Helicoma fumosum (P. Karst.) G.Z. Zhao, Xing Z. Liu & W.P. Wu; Helicofilia irregularis (P.M. Kirk) G.Z. Zhao, Xing Z. Liu & W.P. Wu. Another three new combinations, Helicoma casuarinae (Matsush.) G.Z. Zhao, Xing Z.
    [Show full text]
  • Myconet Volume 14 Part One. Outine of Ascomycota – 2009 Part Two
    (topsheet) Myconet Volume 14 Part One. Outine of Ascomycota – 2009 Part Two. Notes on ascomycete systematics. Nos. 4751 – 5113. Fieldiana, Botany H. Thorsten Lumbsch Dept. of Botany Field Museum 1400 S. Lake Shore Dr. Chicago, IL 60605 (312) 665-7881 fax: 312-665-7158 e-mail: [email protected] Sabine M. Huhndorf Dept. of Botany Field Museum 1400 S. Lake Shore Dr. Chicago, IL 60605 (312) 665-7855 fax: 312-665-7158 e-mail: [email protected] 1 (cover page) FIELDIANA Botany NEW SERIES NO 00 Myconet Volume 14 Part One. Outine of Ascomycota – 2009 Part Two. Notes on ascomycete systematics. Nos. 4751 – 5113 H. Thorsten Lumbsch Sabine M. Huhndorf [Date] Publication 0000 PUBLISHED BY THE FIELD MUSEUM OF NATURAL HISTORY 2 Table of Contents Abstract Part One. Outline of Ascomycota - 2009 Introduction Literature Cited Index to Ascomycota Subphylum Taphrinomycotina Class Neolectomycetes Class Pneumocystidomycetes Class Schizosaccharomycetes Class Taphrinomycetes Subphylum Saccharomycotina Class Saccharomycetes Subphylum Pezizomycotina Class Arthoniomycetes Class Dothideomycetes Subclass Dothideomycetidae Subclass Pleosporomycetidae Dothideomycetes incertae sedis: orders, families, genera Class Eurotiomycetes Subclass Chaetothyriomycetidae Subclass Eurotiomycetidae Subclass Mycocaliciomycetidae Class Geoglossomycetes Class Laboulbeniomycetes Class Lecanoromycetes Subclass Acarosporomycetidae Subclass Lecanoromycetidae Subclass Ostropomycetidae 3 Lecanoromycetes incertae sedis: orders, genera Class Leotiomycetes Leotiomycetes incertae sedis: families, genera Class Lichinomycetes Class Orbiliomycetes Class Pezizomycetes Class Sordariomycetes Subclass Hypocreomycetidae Subclass Sordariomycetidae Subclass Xylariomycetidae Sordariomycetes incertae sedis: orders, families, genera Pezizomycotina incertae sedis: orders, families Part Two. Notes on ascomycete systematics. Nos. 4751 – 5113 Introduction Literature Cited 4 Abstract Part One presents the current classification that includes all accepted genera and higher taxa above the generic level in the phylum Ascomycota.
    [Show full text]
  • Tubeufiaceae) from Freshwater Habitats
    Mycosphere 8(9): 1443–1456 (2017) www.mycosphere.org ISSN 2077 7019 Article Doi 10.5943/mycosphere/8/9/9 Copyright © Guizhou Academy of Agricultural Sciences Neotubeufia gen. nov. and Tubeufia guangxiensis sp. nov. (Tubeufiaceae) from freshwater habitats Chaiwan N1, Lu YZ1,5, Tibpromma S1,2,3,4, Bhat DJ6, Hyde KD1,2,3,4 and Boonmee S1* 1 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand 2 Mushroom Research Foundation, 128 M.3 Ban Pa Deng T. Pa Pae, A. Mae Taeng, Chiang Mai 50150, Thailand 3 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, Yunnan, People’s Republic of China 4 World Agroforestry Centre, East and Central Asia, Kunming 650201, Yunnan, P. R. China 5 Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guizhou University, Guiyang, Guizhou Province 550025, P.R. China . 6 Formerly at Department of Botany, Goa University, Goa, 403206, India Chaiwan N, Lu YZ, Tibpromma S, Bhat DJ, Hyde KD, Boonmee S 2017 – Neotubeufia gen. nov. and Tubeufia guangxiensis sp. nov. (Tubeufiaceae) from freshwater habitats. Mycosphere 8(9), 1443–1456, Doi 10.5943/mycosphere/8/9/9 Abstract During our fungal forays in freshwater streams of Thailand and China, two new taxa belonging to the family Tubeufiaceae were isolated from decaying submerged wood samples. A new genus Neotubeufia is introduced to accommodate a new species, N. krabiensis, which is comparable to Tubeufia in the features of ascomata, asci and ascospores, but found distinct with dark ascomata, cylindrical asci and cylindric-fusiform ascospores.
    [Show full text]
  • Proposed Generic Names for Dothideomycetes
    Naming and outline of Dothideomycetes–2014 Nalin N. Wijayawardene1, 2, Pedro W. Crous3, Paul M. Kirk4, David L. Hawksworth4, 5, 6, Dongqin Dai1, 2, Eric Boehm7, Saranyaphat Boonmee1, 2, Uwe Braun8, Putarak Chomnunti1, 2, , Melvina J. D'souza1, 2, Paul Diederich9, Asha Dissanayake1, 2, 10, Mingkhuan Doilom1, 2, Francesco Doveri11, Singang Hongsanan1, 2, E.B. Gareth Jones12, 13, Johannes Z. Groenewald3, Ruvishika Jayawardena1, 2, 10, James D. Lawrey14, Yan Mei Li15, 16, Yong Xiang Liu17, Robert Lücking18, Hugo Madrid3, Dimuthu S. Manamgoda1, 2, Jutamart Monkai1, 2, Lucia Muggia19, 20, Matthew P. Nelsen18, 21, Ka-Lai Pang22, Rungtiwa Phookamsak1, 2, Indunil Senanayake1, 2, Carol A. Shearer23, Satinee Suetrong24, Kazuaki Tanaka25, Kasun M. Thambugala1, 2, 17, Saowanee Wikee1, 2, Hai-Xia Wu15, 16, Ying Zhang26, Begoña Aguirre-Hudson5, Siti A. Alias27, André Aptroot28, Ali H. Bahkali29, Jose L. Bezerra30, Jayarama D. Bhat1, 2, 31, Ekachai Chukeatirote1, 2, Cécile Gueidan5, Kazuyuki Hirayama25, G. Sybren De Hoog3, Ji Chuan Kang32, Kerry Knudsen33, Wen Jing Li1, 2, Xinghong Li10, ZouYi Liu17, Ausana Mapook1, 2, Eric H.C. McKenzie34, Andrew N. Miller35, Peter E. Mortimer36, 37, Dhanushka Nadeeshan1, 2, Alan J.L. Phillips38, Huzefa A. Raja39, Christian Scheuer19, Felix Schumm40, Joanne E. Taylor41, Qing Tian1, 2, Saowaluck Tibpromma1, 2, Yong Wang42, Jianchu Xu3, 4, Jiye Yan10, Supalak Yacharoen1, 2, Min Zhang15, 16, Joyce Woudenberg3 and K. D. Hyde1, 2, 37, 38 1Institute of Excellence in Fungal Research and 2School of Science, Mae Fah Luang University,
    [Show full text]
  • Fungi of Ding Darling Wildlife Refuge, Florida
    A Preliminary Report of the Microfungi of Ding Darling Wildlife Refuge (Sanibel Island, Florida), December 2010 Adrian Carter: Research Associate New Brunswick Museum; Volunteer Ding Darling Wildlife Refuge Introduction: The J. N. "Ding" Darling National Wildlife Refuge, administered by the US Fish and Wildlife Service, is located on the subtropical barrier island of Sanibel in the Gulf of Mexico, just west of Ft. Myers in Lee County, Florida. The refuge is part of the largest undeveloped mangrove ecosystem in the United States and is world famous for its spectacular migratory bird populations Long recognized as unique, numerous reports, surveys and checklists of the natural area have been generated. An excellent summary of the mangrove uplands and interior wetlands of Sanibel Island including Ding Darling can be found in John Clark's The Sanibel Report (Clark, 1976), now out of print but available through the internet. Since that time the area has developed into a tourist haven with the resulting impact of several thousand visitors per year. As recently as 2008 the US Fish and Wildlife Service initiated a comprehensive conservation plan and environmental assessment for Ding Darling announced in the Federal Register April 2, 2008 (Vol. 73, Number 64, pp. 17991-77912). While there is a great deal of interest in the area, a survey of the microfungi (Ascomycetes and their anamorphs) of the Ding Darling Wildlife Refuge has never been conducted. The current report is a preliminary attempt to address that gap. The information contained in the following report is useful not just from a biodiversity perspective, but can be used to address key initiatives in a conservation plan.
    [Show full text]
  • Three New Species of Acanthostigma (Tubeufiaceae, Dothideomycetes) from Great Smoky Mountains National Park
    Mycologia, 102(3), 2010, pp. 574–587. DOI: 10.3852/09-051 # 2010 by The Mycological Society of America, Lawrence, KS 66044-8897 Three new species of Acanthostigma (Tubeufiaceae, Dothideomycetes) from Great Smoky Mountains National Park Itthayakorn Promputtha cellular pseudoparaphyses, cylindrical to clavate, Andrew N. Miller1 bitunicate asci, and cylindrical-fusiform to elongate- University of Illinois, Illinois Natural History Survey, fusiform, hyaline, transversely multiseptate ascospores 1816 South Oak Street, Champaign, Illinois 61820- (Re´blova and Barr 2000). Members of Acanthostigma 6970 usually occur as saprobes on terrestrial decomposing wood (Rossman 1987, Re´blova and Barr 2000) but also have been found on submerged wood in Abstract: Three new bitunicate ascomycetes belong- freshwater (Kodsueb et al. 2006). Some species are ing to the genus Acanthostigma are described from associated with helicosporous hyphomycete ana- terrestrial decomposing wood collected from Great morphs (Barr 1980, Re´blova and Barr 2000, Kodsueb Smoky Mountains National Park, USA. Phylogenetic et al. 2004, Tsui et al. 2006). analyses of the nuclear ribosomal 28S large subunit During our ongoing study of fungal diversity in and internal transcribed spacer region placed all three Great Smoky Mountains National Park (GSMNP) five species in the Tubeufiaceae and confirmed morpho- species of Acanthostigma were found, three of which logical analyses that these are distinct species. Expand- do not fit the description of any known species. These ed phylogenetic analyses of 28S large subunit includ- three newly discovered species are described, illus- ing taxa throughout the Dothideomycetes confirmed trated and compared morphologically and genetically the placement of Acanthostigma in the Tubeufiaceae. to other known species in the genus.
    [Show full text]
  • Acanthostigma and Tubeufia Species, Including T. Claspisphaeria Sp. Nov
    Mycologia, 96(3), 2004, pp. 667±674. q 2004 by The Mycological Society of America, Lawrence, KS 66044-8897 Acanthostigma and Tubeu®a species, including T. claspisphaeria sp. nov., from submerged wood in Hong Kong Rampai Kodsueb including hyphomycetes (Hyde et al 2002, McKenzie Saisamorn Lumyong1 et al 2002), coelomycetes (Hyde 1993) and ascomy- Department of Biology, Faculty of Science, Chiang Mai cetes (Wong and Hyde 1999, Cai et al 2002). During University, Chiang Mai, Thailand our survey of fungi occurring on naturally sub- merged wood, we collected an Acanthostigma and two Pipob Lumyong Tubeu®a species, one being a species new to science, Department of Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand from a small forest stream in Hong Kong. The three species are described, illustrated and compared with Eric H.C. McKenzie known taxa. A key to the 16 accepted species of Tu- Landcare Research, Private Bag 92170, Aukland, beu®a is provided. New Zealand Wai Hong Ho MATERIALS AND METHODS Kevin D. Hyde Centre for Research in Fungal Diversity, Department of Submerged wood was retrieved from a stream near the Ecology & Biodiversity, The University of Hong Kong, Trappist Monastery, Lantau Island, Hong Kong, in Apr 2002 Pokfulam Road, Hong Kong and returned to the laboratory, where it was incubated in zip-lock plastic bags at room temperature. Humidity was maintained by adding moistened paper towels. Samples Abstract: Acanthostigma scopulum, Tubeu®a claspis- were examined within 3 d and periodically over 1 mo for phaeria sp. nov. and T. paludosa were identi®ed from the presence of sporulating structures.
    [Show full text]
  • Alkaline Protease
    [ -----------/^ | I VOLUME 49 I I / I— I I— I SEPTEMBER 1996 M y c o l o g y 2 CZECH SCIENTIFIC SOCIETY FOR MYCOLOGY PRAHA n I o MV J < ^AYC~ ISSN 0009-0476 I nI .o arío v J < Vol. 49, No. 2, September 1996 CZECH MYCOLOGY formerly Česká mykologie published quarterly by the Czech Scientific Society for Mycology EDITORIAL BOARD Editor-in-Chief ZDENĚK POUZAR (Praha) Managing editor JAROSLAV KLÁN (Praha) VLADIMÍR ANTONÍN (Brno) JIŘÍ KUNERT (Olomouc) OLGA FASSATIOVÁ (Praha) LUDMILA MARVANOVÁ (Brno) ROSTISLAV FELLNER (Praha) PETR PIKÁLEK (Praha) JOSEF HERINK (Mnichovo Hradiště) MIRKO SVRČEK (Praha) ALEŠ LEBEDA (Olomouc) Czech Mycology is an international scientific journal publishing papers in all aspects of mycology. Publication in the journal is open to members of the Czech Scientific Society for Mycology and non-members. Contributions to: Czech Mycology, National Museum, Department of Mycology, Václavské nám. 68, 115 79 P raha 1, Czech Republic. Phone: 02/24497259 SUBSCRIPTION. Annual subscription is Kč 250,- (including postage). The annual sub­ scription for abroad is US $86,- or DM 136,- (including postage). The annual member­ ship fee of the Czech Scientific Society for Mycology (Kč 160,- or US $60,- for foreigners) includes the journal without any other additional payment. For subscriptions, address changes, payment and further information please contact The Czech Scientific Society for Mycology, P.O.Box 106, 111 21 Praha 1, Czech Republic. Copyright © The Czech Scientific Society for Mycology, Prague, 1996 No. 1 of the vol. 49 of Czech Mycology appeared in May 30, 1996 CZECH MYCOLOGY Publication of the Czech Scientific Society for Mycology Volume 49 September 1996 Number 2 The genus Erysiphe in Serbia B r a n is l a v R a n k o v i č a n d L j i l j a n a C o m i c Faculty oof Natural Sciences, University of Kragujevac, 34000 Kragujevac, Yugoslavia Rankovic B.
    [Show full text]
  • <I>Tubeufiaceae</I> from Andean Patagonian Forests of Argentina
    MYCOTAXON Volume 111, pp. 131–141 January–March 2010 New records in the Tubeufiaceae from Andean Patagonian forests of Argentina Romina M. Sánchez & M. Virginia Bianchinotti [email protected] & [email protected] Lab. Ficología y Micología, DBByF, Universidad Nacional del Sur. LEBBAH, CERZOS (CONICET), Edificio E1, Camino La Carrindanga, Km7, B8000FWB, Bahía Blanca, Argentina Abstract — Tubeufiaceae (Pleosporales, Ascomycota) occurring on native trees from the Andean Patagonian forests in Argentina are described and illustrated. Acanthostigma minutum and Tubeufia cerea with its anamorphic state are reported from South America for the first time on Nothofagus dombeyi and N. antarctica, respectively. Both species were up to now only known from the Northern Hemisphere. Key words — ascomycetes, Helicosporium, Nothofagaceae Introduction Barr (1979) erected the family Tubeufiaceae to accommodate several genera of Pleosporales that are saprobic on wood, hypersaprobic or hyperparasitic on other fungi, or parasitic on scale insects. The ascomata are small, pallid yellowish to brownish, globose, conic, ellipsoid or cylindrical. She included six genera in the family: the type genus Tubeufia, Letendraea Sacc., Melioliphila Speg., Podonectria Petch, Rebentischia P. Karst., and Thaxteriella Petr. Later, Barr (1980) added five more genera — Allonecte Syd., Boerlagiomyces Butzin, Byssocallis Syd., Paranectriella (Henn. ex Sacc. & D. Sacc.) Höhn., and Puttemansia Henn. — and synonymized Thaxteriella with Tubeufia. The classification of the Tubeufiaceae, mainly based on morphology, has been controversial. Eriksson (2005) placed this family as “Dothideomycetes et Chaetothyriomycetes incertae sedis”. Based on sequence analyses Kodsueb et al. (2006) considered the Tubeufiaceae a distinct monophyletic family that clusters within the Pleosporales as originally proposed by Barr (1980) and excluded Boerlagiomyces and Letendraea as phylogenetically unrelated.
    [Show full text]
  • Neohelicosporium Fusisporum Sp. Nov. (Tubeufiaceae) and a First Record of a Sexual Morph Within Neohelicosporium
    Studies in Fungi 2 (1): 210–217 (2017) www.studiesinfungi.org ISSN 2465-4973 Article Doi 10.5943/sif/2/1/24 Copyright © Mushroom Research Foundation Neohelicosporium fusisporum sp. nov. (Tubeufiaceae) and a first record of a sexual morph within Neohelicosporium Jayasiri SC 1, Hyde KD 1, Jones EBG 2 and Lu YZ 3 1Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand 2Nantgaredig, 33B St. Edwards Road, Southsea, Hants. PO5 3DH, UK 3Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National 8 Education Ministry of China, Guizhou University, Guiyang, Guizhou Province 550025, P.R. China Jayasiri SC, Hyde KD, Jones EBG, Lu YZ – 2017 Neohelicosporium fusisporum sp. nov. (Tubeufiaceae) and the first report of a sexual morph for the genus. Studies in Fungi 2(1), 210–217, Doi 10.5943/sif/2/1/24 Abstract Both sexual and asexual morphs (holomorph) are known in several genera of Tubeufiaceae whereas in others, mainly the asexual or rarely the sexual morph is known. The genus Neohelicosporium is known only by its asexual morph, we have recently collected the sexual morph of the genus, which is characterized by fleshy, superficial ascomata, bitunicate asci, and hyaline to pale brown, fusiform ascospores. Analyses of combined ITS, LSU and tef1 sequence data placed the taxon in Neohelicosporium. In this paper, we introduce the new taxon N. fusisporum based on both morphological characteristics and phylogenetic data. Keywords – Bitunicate – fusiform – new species – phylogenetic data Introduction The family Tubeufiaceae is an interesting and important group of fungi with 23 genera, that includes saprobic, hyperparasitic or hypersaprobic species on ascomycetes and scale insects (Boonmee et al.
    [Show full text]