DOCSLIB.ORG

AGAR PLATES SABOURAUD DEXTROSE 50>S

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
AGAR PLATES SABOURAUD DEXTROSE 50>S INSTRUCTIONS FOR USE – AGAR PLATES SABOURAUD DEXTROSE 50>S 1092 SPML manufacture and supply a range of Pre-prepared media for the As methods and media preference may vary as to media types and test culture of microorganisms such as bacteria yeasts and moulds. SPML method due to jurisdictions, and personal preferences the customer offer a range of media pre-poured culture media to meet the needs of its should use the plates in accordance with their organisations stated customers across a range of industries that include Clinical, food, water, methods and procedures however SPML product brochure does provide pharmaceutical, and many more. further product information The range of plates cover selective and non-selective media and plate The end user should however take into consideration that selective sizes and are intended for the use by professional parties in the isolation media should, therefore, be compared with specimens/samples cultured of microbial organisms. on nonselective media to obtain additional information and help ensure recovery of potential pathogens and other significant organisms. INTENDED USE An acid pH medium for the isolation of dermatophytes, other fungi and PRECAUTIONS yeasts. Filled in 50mm diameter petridishes. For professional use only. This modification of Sabouraud agar (Carlier1) is suitable for the Do not use plates if they show evidence of microbial contamination, cultivation and differentiation of fungi. Carlier showed that the medium discoloration, drying, cracking or other signs of deterioration. gives reliable results with Microsporum audouini, M. canis, Trichophyton Consult In house instructions to ensure correct application of product mentagrophytes, T. flavum, T. rubrum and Candida albicans. Sabouraud use is observed. Ensure GLP and aseptic handling procedures are Dextrose Agar may be used in place of the Standard American medium followed, used plates should be treated as biohazards, and disposal of of Hodges2. The fungi maintain their typical cultural appearance and used product should be treated as such, and disposed of in accordance thus may be readily identified according to the standard macroscopic with local /national regulations. characters described by Sabouraud3. The medium is often used with antibiotics for the isolation of pathogenic fungi from material containing STORAGE AND SHELF LIFE large numbers of other fungi or bacteria. Georg et al.4 aseptically added On receipt, store plates in the dark at 2 to 8° C, in their original sleeve 0.5 gram cycloheximide, 20,000 units penicillin and 40,000 units wrapping until just prior to use. Avoid freezing and overheating. The streptomycin to each litre of autoclaved, cooled medium. Cryptococcus plates may be inoculated up to the expiration date (see package label) neoformans, Aspergillus fumigatus and Allescheria boydii are sensitive to and incubated for the recommended incubation times. cycloheximide; Actinomyces bovis and Nocardia asteroides are sensitive Plates from opened stacks of 10 plates can be used for one week when to penicillin and streptomycin. Alternatively, one may add 0.4 gram stored in a clean area at 2 to 8° C. chloramphenicol and 0.05 gram cycloheximide to each litre of QUALITY CONTROL reconstituted medium before autoclaving (Ajello5). The same micro- SPML undertake batch release for all media checking pH, sterility and organisms are sensitive to this new combination ± see Dermasel fertility testing of all media batches in accordance with requirements Selective Supplement SR75. Williams Smith &Jones6 employed Oxoid defined in Sabouraud Dextrose Agar, containing 20,000 units penicillin and 0.04 EN12322:199911, M22-A322, and ISO/TS111333 Parts 1 and 23, gram neomycin per litre, for the count of yeasts in the alimentary tract SPML is ISO9001:2008 certified. of the pig. Hantschke7 used colistin, novobiocin and cycloheximide to For clinical purpose the product conform to 97/79/EC IVD Directive isolate Candida albicans. Dolan8 used gentamicin, chloramphenicol and FURTHER INFORMATION cycloheximide for the selective isolation of pathogenic fungi. Oxoid For further information please contact your local SPML representative. Sabouraud Dextrose Agar may also be used as the basis of a Pagano- Saudi Prepared Media Laboratory Company Ltd Levin medium9 for the isolation of Candida albicans. 0.1 gram of Telephone: +966 11 4767931, 11 4773697 triphenyltetrazolium chloride (as a filter sterilised solution) is added to FAX: +966 1 4778313 each litre of autoclaved molten medium cooled to 55oC. The medium is E-mail: [email protected] usually made inhibitory to most non-pathogenic fungi and bacteria by Web Site: http://www.spml.com.sa the addition of antibiotics as above. After incubation for 3 days at 25 oC, Candida albicans colonies are unpigmented or pale pink whilst other Candida species and other fungi form deeper pink or red colonies. The QUALITY CONTROL test is adequate for screening purposes but other diagnostic SPML undertake batch release for all media checking pH, sterility, criteria should also be utilised for the identification of Candida Inhibition and enhancement testing of all media batches based on the 1 2 3 Albicans EN12322:19991 , M22-A3 , and ISO11133 , • Micro Organism Reactions pH: 5.50 -5.70 For clinical purpose the product is marked in accordance with the requirements of 97/79/EC IVD Directive and registered with the Organisms Result, Colour of Colony Competent Authority MHRA in the United Kingdom. Candida Albicans Growth off white, round SPML is ISO9001:2008 certified Escherichia Coli Growth, cream Staphylococcus Aureus No Growth FURTHER INFORMATION For further information please contact your local SPML representative. TECHNIQUE Saudi Prepared Media Laboratory Company Ltd Using a sterile loop inoculate the medium with 4±5 colonies and Telephone: +966 11 4767931, 11 4773697 Incubate one set of media aerobically at 22±25°C and the other set at FAX: +966 1 4778313 35°C for 5±30 days E-mail: [email protected] Web Site: http://www.spml.com.sa SPML-IFU-001 Rev 01 INSTRUCTIONS FOR USE – AGAR PLATES SABOURAUD DEXTROSE 50>S 1092 References. 1 EN 12322:1999 - In vitro diagnostic medical devices - Culture media for microbiology - Performance criteria for culture media. 2 Clinical and Laboratory Standards Institute. 2004. Approved standard: M22-A3, Quality control for commercially prepared microbiological culture. media, 3rd ed 3ISO11133 Microbiology of food, animal feed and water — Preparation, production storage and performance testing of culture media Medical Device & QA Services Ltd (MDQAS), Saudi Prepared Media Laboratory Company Ltd Spring Court, Spring Road, ,Al Harath Bin Al Habab Street 8698 HALE. Cheshire. WA14 2UQ. High-al Dubbath“ Malaz" United Kingdom. Riyadh 11461 Tel: +44 (0) 845 527 5078, Fax: +44 161 903 9787 Email: info [email protected] Web Kingdom Of Saudi Arabia Site: www.mdqas.com Symbols Definition Product Reference Number For in vitro diagnostic use Do not use if damaged Sterile by Moist / Dry heat Non Sterile Single Use Manufacturer Lot Number Bio hazardous material CE Mark – Against the European In Vitro Diagnostic Medical Device Directive 98/79/EC Storage temperature limitation Manufacture date Consult Instructions for use SPML-IFU-001 Rev 01 INSTRUCTIONS FOR USE – AGAR PLATES SABOURAUD DEXTROSE 50>S 1092 Avoid direct sunlight Keep Dry Expiration Date EU Representative WARNING – Consult IFU SPML-IFU-001 Rev 01 .
Load more

Recommended publications
  • Fermented Rapeseed Meal As a Component of the Mink Diet (Neovison Vison) Modulating the Gastrointestinal Tract Microbiota
    animals Article Fermented Rapeseed Meal as a Component of the Mink Diet (Neovison vison) Modulating the Gastrointestinal Tract Microbiota Łukasz Wlazło 1 , Bozena˙ Nowakowicz-D˛ebek 1,* , Anna Czech 2 , Anna Chmielowiec-Korzeniowska 1, Mateusz Ossowski 1,* , Marek Kułazy˙ ´nski 3,4 , Marcin Łukaszewicz 4,5 and Anna Krasowska 4,5 1 Department of Animal Hygiene and Environmental Hazards, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland; [email protected] (Ł.W.); [email protected] (A.C.-K.) 2 Department of Biochemistry and Toxicology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland; [email protected] 3 Department of Fuel Chemistry and Technology, Faculty of Chemistry, Wrocław University of Science and Technology, Gda´nska7/9, 50-344 Wrocław, Poland; [email protected] 4 InventionBio, Wojska Polskiego 65, 85-825 Bydgoszcz, Poland; [email protected] (M.Ł.); [email protected] (A.K.) 5 Department of Biotransformation, Faculty of Biotechnology, University of Wroclaw, F. Joliot-Curie 14A, 50-383 Wrocław, Poland * Correspondence: [email protected] (B.N.-D.); [email protected] (M.O.); Tel.: +48-81-445-69-98 (B.N.-D.); +48-81-445-69-85 (M.O.) Citation: Wlazło, Ł.; Nowakowicz-D˛ebek,B.; Czech, A.; Simple Summary: The high protein requirement in the diet of mink is currently met using extruded Chmielowiec-Korzeniowska, A.; Ossowski, M.; Kułazy´nski,M.;˙ cereals, meat and bone meal, which raises the cost of mink farming. At the same time, there are waste Łukaszewicz, M.; Krasowska, A.
    [Show full text]
  • TS Sabouraud + Actidione + Chloramphenico Agar V3 11-08-11
    V3 – 11/08/11 Sabouraud + Actidione 355-6559 Chloramphenicol/Agar DEFINITION Trichophyton mentagrophytes; Sabouraud agar with the addition of actidione Trichophyton rubrum; and chloramphenicol is recommended for the Epidermophyton flocosum etc. isolation of Dermatophytes and other pathogenic fungi from heavily-contaminated However, this medium does not allow the specimens. isolation of Cryptococcus neoformans or Monosporium apiospermmum . PRESENTATION • Ready-to-use Chloramphenicol inhibits most bacterial 100 ml x 6 bottles code 355-6559 contaminants. PERFORMANCES / QUALITY CONTROL OF THEORETICAL FORMULA THE TEST Peptone 10 g The growth performances of the media are Glucose 20 g verified with the following strains: Actidione (cycloheximide) 0.5 g Performance 24-48H Chloramphenicol 0.5 g STRAINS Agar 15 g at 30-35°C Distilled water 1,000 ml Candida albicans Good growth, white Final pH (25°C) = 6. 0 ± 0.2 ATCC 26790 Candida tropicalis STORAGE Inhibited • Ready-to-use: + 2°C to 25 °C ATCC 750 • Expiration date and batch number are shown on the package. Candida glabrata Inhibited PROTOCOL 7 days at 30-35°C and Inoculation and incubation STRAINS Proceed to isolate of the specimen to be 7 days at 20-25°C analyzed or its decimal dilutions on the Sabouraud + Actidione + Chloramphenicol Good growth downy, Trichophyton rubrum agar. Incubate at 32°C for 3-7 days back red-brown PRECAUTIONS Trichophyton Good growth Comply with Good Laboratory Practice. violaceum Violet pigment UTILISATION Epidermophyton Good growth, powdery, Actidione inhibits the development of certain floccosum brown back light brown fungi ( Candida krusei. Candida tropicalis, Fusarium, aspergillus fumigatus , etc) and has Good growth, downy, Microsporum canis no action on the following pathogenic fungi, back yellow-orange which can be isolated on this medium: All Dermatophytes; Fusarium Inhibited All Candida (except for C.
    [Show full text]
  • Evaluation of Routin Microbiological Media and a Selective Fungal Medium for Recovery of Yeast from Mixed Clinical Specimens
    World Journal of Medical Sciences 1 (2): 147-150, 2006 ISSN 1817-3055 © IDOSI Publications, 2006 Evaluation of Routin Microbiological Media and a Selective Fungal Medium for Recovery of Yeast from Mixed Clinical Specimens 12Abbas Ali Jafari1, Abdul Hossein Kazemi and 3Hossein Zarrinfar 1Parasitology and Mycology Group, College of Medicine, Yazd Medical University, Safaieh Bo Ali Street, Yazd, Iran 2Tabriz Medical University, Immunology Group, Tabriz, Iran 3Parasitology and Mycology Group, College of Medicine, Yazd Medical University, Safaieh Bo Ali Street, Yazd, Iran Abstract: The recovery of yeast from clinical specimens cultured on routine bacteriological media was compared with recovery on a selective fungal medium. Overgrowing of bacteria in mixed bacterial and yeast specimens was suppressing the yeast growth on bacteriological media. Totally 229 pus specimens used for evaluating of bacteriological and fungal selective media. The specimens were cultured on bacteriologic media (blood and chocolate agar) as well as on Sabouraud agar containing Chloramphenicol (50 mg l 1) to inhibit bacterial growth. Finally the yeast growth was reported semi-quantitatively as light, moderate, or heavy and the results analyzed using SPSS software. The number of specimens yielding yeast growth in bacterial and sabouraud agar was compared using Chi-square test. Using Sabouraud agar particularly in cases of mixed infections was very useful for recovering of yeast, because yeast was only recovered from 29.3% of 41 yeast-positive pus specimens (Chi-square = 7.74, Pval = 0.005) and also from 24% of 25yeast-positive throat specimens (Chi-square = 11.09, Pval = 0.00008) using bacteriologic cultures. Using selective fungal medium for culturing of specimens containing a mixture of bacteria and yeasts is very helpful and necessary for accurate detection of yeasts.
    [Show full text]
  • Respiratory Microbiome of Endangered Southern Resident
    www.nature.com/scientificreports OPEN Respiratory Microbiome of Endangered Southern Resident Killer Whales and Microbiota of Received: 24 October 2016 Accepted: 27 February 2017 Surrounding Sea Surface Microlayer Published: xx xx xxxx in the Eastern North Pacific Stephen A. Raverty1,2, Linda D. Rhodes3, Erin Zabek1, Azad Eshghi4,7, Caroline E. Cameron4, M. Bradley Hanson3 & J. Pete Schroeder5,6 In the Salish Sea, the endangered Southern Resident Killer Whale (SRKW) is a high trophic indicator of ecosystem health. Three major threats have been identified for this population: reduced prey availability, anthropogenic contaminants, and marine vessel disturbances. These perturbations can culminate in significant morbidity and mortality, usually associated with secondary infections that have a predilection to the respiratory system. To characterize the composition of the respiratory microbiota and identify recognized pathogens of SRKW, exhaled breath samples were collected between 2006– 2009 and analyzed for bacteria, fungi and viruses using (1) culture-dependent, targeted PCR-based methodologies and (2) taxonomically broad, non-culture dependent PCR-based methodologies. Results were compared with sea surface microlayer (SML) samples to characterize the respective microbial constituents. An array of bacteria and fungi in breath and SML samples were identified, as well as microorganisms that exhibited resistance to multiple antimicrobial agents. The SML microbes and respiratory microbiota carry a pathogenic risk which we propose as an additional, fourth putative stressor (pathogens), which may adversely impact the endangered SRKW population. Killer whales (Orcinus orca) are among the most widely distributed marine mammals in the world with higher densities in the highly productive coastal regions of higher latitudes. In the eastern North Pacific, the Southern Resident Killer Whale (SRKW) population ranges seasonally from Monterey Bay, California to the Queen Charlotte Islands, British Columbia.
    [Show full text]
  • BD Sabouraud Glucose Agar • BD Sabouraud Agar With
    INSTRUCTIONS FOR USE – READY-TO-USE PLATED MEDIA PA-254039.08 Rev.: October 2019 BD Sabouraud Glucose Agar • BD Sabouraud Agar with Chloramphenicol • BD Sabouraud Agar with Gentamicin and Chloramphenicol • BD Sabouraud Agar with Penicillin and Streptomycin • INTENDED USE BD Sabouraud Glucose Agar is used for the isolation and cultivation of fungi (yeasts, molds, and dermatophytes) from clinical specimens. BD Sabouraud Agar with Chloramphenicol, BD Sabouraud Agar with Gentamicin and Chloramphenicol, and BD Sabouraud Agar with Penicillin and Streptomycin are selective media for the isolation of fungi from clinical specimens. PRINCIPLES AND EXPLANATION OF THE PROCEDURE Microbiological method. Sabouraud Glucose Agar is a general purpose medium originally devised for the cultivation of dermatophytes.1,2 Today it is used for the isolation and cultivation of all fungi.3-5 The peptones in Sabouraud Glucose Agar are sources of nitrogenous growth factors. Glucose provides an energy source for the growth of microorganisms. The high glucose concentration provides an advantage for the growth of the (osmotically stable) fungi while most bacteria do not tolerate the high sugar concentration. In addition, the low pH is optimal for fungi, but not for many bacteria.3 Sabouraud Glucose Agar is only slightly selective against bacteria. BD Sabouraud Agar with Chloramphenicol, BD Sabouraud Agar with Gentamicin and Chloramphenicol, and BD Sabouraud Agar with Penicillin and Streptomycin are selective media based on Sabouraud Glucose Agar. Selective agents to inhibit bacteria have been added. Chloramphenicol is a broad-spectrum antibiotic inhibitory to a wide range of gram-negative and gram-positive bacteria but may have an inhibitory effect on several pathogenic fungi.4 Antimicrobials like penicillin, gentamicin, and streptomycin, or a combination of these, have been shown to be effective in inhibiting bacteria without affecting fungal growth.2-5 These media are used for the isolation of fungi from clinical specimens or materials suspected to contain bacterial contaminants.
    [Show full text]
  • The Use of a Single Growth Medium for Environmental Monitoring Of
    European Journal of Parenteral & Pharmaceutical Sciences 2016; 21(2): 50-55 © 2016 Pharmaceutical and Healthcare Sciences Society The use of a single growth medium for environmental monitoring of pharmacy aseptic units using tryptone soya agar with 1% glucose John Rhodes1*, Jennifer Feasby1, Wayne Goddard1, Alison Beaney2 and Mike Baker3 1 North Tees and Hartlepool NHS Foundation Trust, Stockton-on-Tees, UK 2 Newcastle Upon Tyne Hospitals NHS Foundation Trust, Newcastle-upon-Tyne, UK 3 Pharma Quality Consulting, Cheshire, UK The suitability of tryptone soya agar, Sabouraud dextrose agar and tryptone soya agar with 1% glucose plates for general environmental monitoring was compared. Plates were incubated at three different temperatures to assess an optimal temperature for growth. Results indicated that there are benefits from using tryptone soya agar with 1% glucose incubated at 25°C as an all-purpose medium for environmental monitoring. Key words: Tryptone soya agar, Sabouraud dextrose agar, tryptone soya agar with 1% glucose, environmental monitoring of aseptic rooms, settle plates, finger dabs. Introduction any test methods or suitable media. For cleanroom monitoring of UK National Health Service (NHS) aseptic services, more The choice of a microbiological growth medium is not simple. detailed guidance has been provided4 for environmental settle The Difco Manual has proven to be a comprehensive source of plate agars. It indicates that the media recommended is: information since the first edition appeared in 1927. The tenth edition was published in 1984 and found its way into most 1 “standardised on tryptone soya for bacterial count (this microbiology laboratories . The guide for the selection of will also detect yeasts and moulds to an extent) and culture media formed a 9-page table and contained many agars Sabouraud dextrose medium for the selective and broths for isolation, differentiation and propagation of determination of yeasts and moulds.” 4 different classes of micro-organisms.
    [Show full text]
  • BD Industry Catalog
    PRODUCT CATALOG INDUSTRIAL MICROBIOLOGY BD Diagnostics Diagnostic Systems Table of Contents Table of Contents 1. Dehydrated Culture Media and Ingredients 5. Stains & Reagents 1.1 Dehydrated Culture Media and Ingredients .................................................................3 5.1 Gram Stains (Kits) ......................................................................................................75 1.1.1 Dehydrated Culture Media ......................................................................................... 3 5.2 Stains and Indicators ..................................................................................................75 5 1.1.2 Additives ...................................................................................................................31 5.3. Reagents and Enzymes ..............................................................................................75 1.2 Media and Ingredients ...............................................................................................34 1 6. Identification and Quality Control Products 1.2.1 Enrichments and Enzymes .........................................................................................34 6.1 BBL™ Crystal™ Identification Systems ..........................................................................79 1.2.2 Meat Peptones and Media ........................................................................................35 6.2 BBL™ Dryslide™ ..........................................................................................................80
    [Show full text]
  • Isolation and Identification of Microorganisms in Eggs of a Commercial Ostrich Breeder Farm
    Journal of Animal Science and Research SciO p Forschene n HUB for Sc i e n t i f i c R e s e a r c h ISSN 2576-6457 | Open Access RESEARCH ARTICLE Volume 4 - Issue 3 Isolation and Identification of Microorganisms in Eggs of a Commercial Ostrich Breeder Farm Alireza Behnamifar1, Shaban Rahimi1,*, Mohammad Ali Akhavizadegan2, Mohammad Amir Karimi Torshizi1, and Anita Maleki1 1Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Iran 2Razi Serum and Vaccine Production Research Institute, Agriculture Research, Education and Extension Organization (AREEO), Iran *Corresponding author: Shaban Rahimi, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran, Tel: +982148292358; E-mail: [email protected] Received: 01 Jul, 2020 | Accepted: 28 Jul, 2020 | Published: 04 Aug, 2020 Citation: Behnamifar A, Rahimi S, Akhavizadegan MA, Karimi Torshizi MA, Maleki A (2020) Isolation and Identification of Microorganisms in Eggs of a Commercial Ostrich Breeder Farm. J Anim Sci Res 4(3): dx.doi.org/10.16966/2576-6457.143 Copyright: © 2020 Behnamifar A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract Contamination of hatching eggs with pathogenic microorganisms decrease hatchability and may cause a financial loss in industrial bird production. The aim of this study was to evaluate the microbiological status of a commercial ostrich farm and its relationship to the hatchery management. Microbial sampling was done from the organs of two dead embryos, cloacae of seven newly hatched chicks and one mature female ostrich, the content of five infertile eggs, the incubation room and incubator machines, the ingredients of diet, and the shell surface of 62 eggs which were laid during a reproductive season.
    [Show full text]
  • EOSIN METHYLENE BLUE AGAR (EMB) EMB Agar, a Differential
    EOSIN METHYLENE BLUE AGAR (EMB) EMB agar, a differential & selective plating medium, is recommended for the detection and isolation of the gram-negative intestinal pathogenic bacteria. The combination of eosin and methylene blue as indicators gives sharp and distinct differentiation between colonies of lactose fermenting organisms and those which do not ferment lactose. The contents of this medium include peptone, lactose, sucrose, dipotassium phosphate, agar, eosin and methylene blue. Today EMB agar is one of the most frequently used agars to detect bacteria found in urine specimens, especially E. Coli. Obviously both organisms are gram- negative. Remember EMB agar inhibits the growth of gram-positive organisms. NUTRIENT AGAR Nutrient agar is recommended as a general culture medium for the cultivation of the majority of the less fastidious microorganisms, as well as a base to which a variety of materials are added to give selective differential, or enriched media. Today this media is gradually begin replaced by Tryptic Soy agar, however, it is included in this demonstration on media since this is on the media you will be inoculating in next week’s lab. Tryptic Soy agar is basically the same, however, a greater number of organism can be grown on it. Nutrient agar is used for the ordinary routine examinations of water, sewage, and food products; for the carrying of stock cultures; for the preliminary cultivation of samples submitted for bacteriological examination; and for isolating organisms in pure culture. The basic ingredients of Nutrient agar include beef extract, peptone and agar. CHOCOLATE AGAR Chocolate agar is recommended for the cultural isolation of Neisseria gonorrhoeae from chronic and acute cases of gonorrhoea in the male and female and in other gonococcal infections.
    [Show full text]
  • Ready to Use Culture Media in 3 L Or 5 L Bags
    ® Product Catalogue 2017 © Liofilchem® s.r.l. Liofilchem Roseto degli Abruzzi, Italy Est. 1983 Clinical and Industrial Microbiology Ready culture media in 55 mm contact plates Individually packed in transparent blister # Product with minimum order Description Packaging Ref. Baird Parker Agar + Neutralizing 20 plates 15331 Selective medium for Staphylococcus aureus isolation with inactivation of disinfectants. Cetrimide Agar E.P. 20 plates 15332 Selective medium for Pseudomonas aeruginosa isolation. Cetrimide Agar + Neutralizing E.P. 20 plates 15386 # Selective medium for Pseudomonas aeruginosa isolation with inactivation of disinfectants. Legionella BCYE Agar ISO 11731 20 plates 15334 Non-selective medium for Legionella spp. isolation. ISO 11731-2 Legionella GVPC Agar ISO 11731 20 plates 15376 Selective medium for Legionella spp. isolation. ISO 11731-2 MacConkey Agar E.P. 20 plates 15329 Selective medium for gram-negatives isolation. Mannitol Salt Agar EP, USP, JP 20 plates 15328 Selective medium for staphylococci isolation. m-Faecal Coliform Agar 20 plates 15368 Selective medium for fecal coliforms isolation and enumeration. Plate Count Agar ISO 4833 20 plates 15325 Medium for surfaces and air total bacterial count. Plate Count Agar + Neutralizing ISO 4833 20 plates 15336 Medium for surfaces total bacterial count with inactivation of disinfectants. Plate Count Agar + Neutralizing (Irradiated) ISO 4833 20 plates 15336S Medium for surfaces total bacterial count with inactivation of disinfectants. Plate Count Agar + TTC + Neutralizing ISO 4833 20 plates 15360 Medium for surfaces total bacterial count with inactivation of disinfectants. Rose Bengal Agar + CAF APHA 20 plates 15374 Selective medium for yeasts and moulds isolation and enumeration. Rose Bengal Agar + CAF + Neutralizing 20 plates 15385 # Selective medium for yeasts and moulds isolation and enumeration.
    [Show full text]
  • Direct Identification of Clinically Relevant Microorganisms
    Direct identification of clinically Application Note Raman relevant microorganisms on Spectroscopy solid culture media by Raman Biology spectroscopy RA62 I. Espagnona, D. Ostrovskiib, R. Matheyb, M. Dupoyc, P. Jolyc, A. Novelli-Rousseaub, F. Pinstonb, O. Gala, F. Mallardb, D. Lerouxd aCEA, LIST, Département Métrologie, Instrumentation et Information, Gif-sur-Yvette, France, bbioMérieux, Technology Research Dept. Grenoble, France, cCEA, LETI, MINATEC, Grenoble, France, d bioMérieux, Technology Research Dept., Marcy l’Etoile, France Abstract Decreasing turnaround time is a paramount objective in clinical diagnosis. We evaluated the discrimination power of Raman spectroscopy when analyzing microcolonies from nine bacterial and one yeast species directly on solid culture medium after a shortened incubation time of 6 h (compared to 24 hour for macrocolonies). This approach, that minimizes sample preparation and culture time, would allow resuming culture after identification to perform downstream antibiotic susceptibility testing. Keywords Raman Spectroscopy, Rapid microbiology, Bacteria colonies, Culture medium, in vitro diagnostics Introduction outside (Acinetobacter baumannii, A. johnsonii, and Stenotrophomonas maltophilia); Gram-positive bacterial (Bacillus cereus, Staphylococcus In vitro microbiological diagnostics are still heavily relying on time- aureus, and S. epidermidis) and one yeast species, selected as a consuming cultivation of microorganisms to identify infectious agents eukaryotic outlier (Candida albicans). Eight well-characterized
    [Show full text]
  • CDC Anaerobe 5% Sheep Blood Agar with Phenylethyl Alcohol (PEA) CDC Anaerobe Laked Sheep Blood Agar with Kanamycin and Vancomycin (KV)
    Difco & BBL Manual Manual of Microbiological Culture Media Second Edition Editors Mary Jo Zimbro, B.S., MT (ASCP) David A. Power, Ph.D. Sharon M. Miller, B.S., MT (ASCP) George E. Wilson, MBA, B.S., MT (ASCP) Julie A. Johnson, B.A. BD Diagnostics – Diagnostic Systems 7 Loveton Circle Sparks, MD 21152 Difco Manual Preface.ind 1 3/16/09 3:02:34 PM Table of Contents Contents Preface ...............................................................................................................................................................v About This Manual ...........................................................................................................................................vii History of BD Diagnostics .................................................................................................................................ix Section I: Monographs .......................................................................................................................................1 History of Microbiology and Culture Media ...................................................................................................3 Microorganism Growth Requirements .............................................................................................................4 Functional Types of Culture Media ..................................................................................................................5 Culture Media Ingredients – Agars ...................................................................................................................6
    [Show full text]