• Abstract and Figures
• Public Full-text

Kinetic and Pharmacological Properties of the Sodium Channel of Frog Skeletal Muscle DONALD T. CAMPBELL and BERTIL HILLE From the Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington 98195. Dr. Campbell's present address is the Department of Physiology, Yale University School of Medicine, New Haven, Connecticut 06510. A B S T R A C T Na channels of frog skeletal muscle are studied under voltage clamp and their properties compared with those of frog myelinated nerve. A standard mathematical model is fitted to the sodium currents measured in nerve and in muscle to obtain a quantitative description of the gating kinetics. At 5°C the kinetics in frog nerve and skeletal muscle are similar except that activation proceeds five times faster in nerve. Block of Na channels by saxitoxin is measured in nerve and in muscle. The apparent dissociation constants for the inhibitory complex are about 1 nM and not significantly different in nerve and muscle. Block of Na channels by external protons in muscle is found to have an apparent pKa of 5.33 and a voltage dependence corresponding to action of 27% of the membrane potential drop. Both values are like those for nerve. Shift of the peak sodium permeability-membrane potential curve with changes of external pH and Ca ++ are found to be the same in nerve and muscle. It is concluded that Na channels of nerve and muscle are nearly the same. INTRODUCTION This paper examines properties of the Na channel of frog muscle membranes under voltage clamp conditions. The guiding question to ask is if the Na channel of muscle is similar to that of nerves taken from the same species. For this reason the focus is on properties already well described in frog myelinated nerve. These include the kinetics of opening and closing of channels, the affinity of channels for saxitoxin and H + ions, and shifts of gating as the external concentration of Ca ++ and H + ions is varied. The results show an overwhelming similarity in the two tissues, and we conclude that the channels may comprise the same molecular species. METHODS The experiments were done on fiber fragments from the semitendinosus muscle of the frog Rana pipiem, prepared and placed under voltage clamp by the method given in the first paper (Hille and Campbell, 1976). The notation of that paper is used here. The object of the first series of experiments to be described was to measure the rates of activation and inactivation of Na channels under voltage clamp in order to develop a mathematical description of gating kinetics in muscle. The empirical kinetic model used follows the approach originated by Hodgkin and Huxley (1952) and applied by Adrian et THE JOURNAL OF GENERAL PHYSIOLOGY " VOLUME 67, 1976 • pages 309-323 309 310 THE JOURNAL OF GENERAL PHYSIOLOGY'VOLUME 67- 1976 al. (1970) and Ildefonse and Roy (1972) to sodium currents of frog skeletal muscle. The time-course of sodium currents is represented by INa(t,E) = [m(t,E)]a[h(t,E)]INa(E), (1) where m and h are parameters ranging from zero to one and "1Na is the hypothetical current-voltage relation that would obtain if m and h were simultaneously equal to one. Changes of m and h are governed by first-order transitions dm dt - a,,~(1 - m) - tim(m), (2) dh = ah(1 - h) - fib(h), (3) with practical parameters m~, ~'m, h=, and % defined by m= = a,,/(ct,, + tim), (4) ~',n = I/(CZm + tim), (5) h= = a~/(ah + Oh), (6) "rn = 1/(0th + ilk)- (7) Finally the voltage dependence of the rate constants is represented by empirical functions of the form used by Adrian et al. (1970): ~,. (e -/~,.) a,,, =1 - exp - [(/~,,, - E)/V,~,,,]' (8) = /],, exp [(/~,,, - E)/V~,,], (9) = &h exp [(Eh - E)/V~,h], (10) flh -1 + exp [(/~h - E)/Voh]" (!1) Membrane currents for the kinetic experiments were recorded on-line with a minicom- puter kindly built for us by Dr. T. H. Kehl and his staff (Kehl et al., 1975). All the refinements of the voltage clamp reported before were used in these studies, e.g., R.~ compensation, ZED compensation, leak and capacity subtraction, etc. Membrane currents were sampled every 30/xs and recorded on digital magnetic tape. Then sodium currents during depolarizing test pulses were fitted to the following formula, simplified from Eqs. 1-7 INa(t) = a(l - e-tl'm) 3 e -",h, (12) where A is an amplitude factor. The equation assumes that m at the beginning of the test pulse is zero, a very good assumption, and that h inactivates fully to zero in the steady state, a less perfect assumption. A computer program in BASIC selected values of A, %,, and 7h to minimize the sum of the squared differences between the observations and Eq. 12 using the Patternsearch routine (Colquhoun, 1971, p. 263) for minimization. As is described in the Results section, appropriate values for the constants in Eqs. 9-11 were then chosen to describe the voltage dependence of Tin, ~'h, the peak sodium current, and h~ measured using various prepulses. A second series of experiments was designed to measure the block of Na channels by externally applied saxitoxin. The solutions contained 0, 0.5, and 2.0 nM saxitoxin, 50 mM D. T. CAMPBELL AND B. HILLE Na Channel of Frog Skeletal Muscle 311 tetraethylammonium chloride, 60 mM NaCl, 2 mM CaClz, and 6 mM tris(hydroxymethyl)aminomethane buffer at pH 7.4. The saxitoxin was kindly provided by Dr. E. J. Schantz. A third series of experiments was designed to measure the effects of H + ions and Ca ++ ions on the voltage dependence and amplitude of peak sodium permeability. Bathing solutions were of two types. One had a fixed pH of 7.4 and five concentrations of Ca++: 0, 0.5, 2, 5, and 20 mM. The other had a fixed 2 mM Ca ++ and five pH values: 4.5, 5.2, 6.0, 7.4, and 9.4. The low pH buffer was piperazine + propionic acid, the neutral buffer was tris(hydroxymethyl)aminomethane + HCI, and the high pH buffer was tris(hydroxymethyl)aminomethane + cyclohexylaminopropanesulfonic acid. All solu- tions contained 60 mM NaCI, 50 mM tetraethylammonium chloride, and 10 mM buffer in addition to CaCI2. Sodium concentration was kept low to reduce errors from large currents flowing in the extracellular series resistance, since this series of experiments was done before the series resistance compensator had been developed. All test depolarizing steps were preceded by 60-ms 45-mV hyperpolarizations to reduce ordinary sodium inactivation. The holding potential was also increased in low-calcium solutions to avoid the effects of long-term sodium inactivation. Potentials were corrected for junction potentials. As described by Hille et al. (1975), peak sodium permeability was calculated from Na currents with the Goldman (1943) and Hodgkin and Katz (1949) equation, and the changes of peak PNa versus E curves were analyzed by the model of Woodhull (1973) as described later. RESULTS Gating in Na Channels of Muscle The time-course of sodium currents under voltage clamp was measured in several muscle fibers at 5°C. A partial family of currents from the most exten- sively studied fiber is shown in Fig. 1 on two time scales. The traces are drawn by computer from digitized on-line recordings after automatic subtraction of leak- age and capacity currents. The time constants rm and rh and the steady-state values of m and h were then calculated in order to develop a complete kinetic model for sodium permeability changes in muscle. The stepwise determination of the required parameters is now described. INACTIVATION OF NA CHANNELS The voltage dependence of h® was deter- mined by measuring the effect of various 200-ms conditioning prepulses on the peak sodium current elicited in a test pulse to near 0 mV. Results from three muscle fibers are plotted as symbols in Fig. 2 A. At the holding potential of -90 mV sodium channels are 10-30% inactivated and the potential for half inactiva- tion is between -75 and -84 inV. The smooth curve is drawn from the kinetic model described later. Eq. 12 was fitted to the first 100-200 points (3.0-6.0 ms) of the digitized voltage clamp currents to determine the Hodgkin-Huxley time constants rm and rh for activation and inactivation of sodium channels. In general the fit to Eq. 12 was very good with a standard deviation of the points from the fitted curve of less than 5% of the peak amplitude. A modification of Eq. 12 including a factor for incomplete sodium inactivation was also fitted to the observations to test if inactivation was incomplete for large depolarizations as reported by Ildefonse and Roy (1972). For steps to absolute potentials between -10 and +130 mV the 312 THE JOURNAL OF GENERAL PHYSIOLOGY • VOLUME 67 • 1976 t (ms) t (ms) 0 2 4 6 8 I0 12 0 0.5 I 1.5 2 2.5 3 | I I I I I I I I !o0.5 -0.5 -i .0 FIGURE 1. Time-course of sodium currents in a semitendinosus muscle fiber under voltage clamp at 5°C. The fiber was held at -90 mV, stepped to a prepulse of -135 mV for 50 ms, and then stepped to test pulse potentials ranging from -75 to + 120 inV. Membrane currents corrected for leakage, capacity, and the impedance ZED were sampled on-line digitally every 30/zs and drawn later by computer on two time scales. The currents are small. Records taken earlier on the same fiber gave currents twice as large.

Load more

  15

Copy Link