Symbiont-Conferred Immunity Interacts with the Effects of Parasitoid Genotype and Intraguild

1 Electronic Supplementary Materials

3 Symbiont-conferred immunity interacts with the effects of parasitoid genotype and intraguild

4 predation to shape pea aphid immunity in a clone-specific fashion

5 6 7 Samuel Alexander Purkissa*, Mouhammad Shadi Khudra*, Oscar Enrique Aguinagab, Reinmar Hagera 8 9 10 a School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, M13 11 9PT, Manchester, UK 12 b Departamento de Ingeniería, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru

13 Corresponding author: Reinmar Hager

15 * Equal contribution.

20 Figure S1. Experimental design. The diagram shows the full experimental design, with n sires being mated to at least three dams (D1 – Dm). The sire × dam 21 mating groupings produced the intraspecific genetic variability in the parasitoid (genotype/daughters [sibs, and half-sibs]). Overall, there were 119 parasitoid 22 daughters. Each group of daughters of the (S1 – Sn) combinations was then split into two populations, with one (n = 73) being provided with pea aphids of the 23 N116 lineage as a provision, while the other (n = 45) provided with pea aphids of the Q1 lineage. Each of these populations where further split into two groups, 24 with one group exposed to intraguild predation by the aphid lion larva (n = 43, in the case of N116, and n = 15 in the case of Q1) and the other group not (n = 25 30, in the case of N116, and n = 30 in the case of Q1). The effects of parasitoid and aphid genetic variability (with and without the aphid lion) on aphid immunity 26 ratio (IR) were investigated in microcosms. IR is the proportion of healthy aphids (non-mummified i.e. unparasitoidised) after 11 days of exposure to the 27 parasitoid genotype relative to the entire population of aphids (healthy and mummified) per aphid lineage. 28

29 30 31 Figure S2. Detailed N116 immunity to parasitoid genotype with and without IGP. This ‘sunburst’ infographic illustrates the detailed immunity ratio (IR) in 32 the N116 pea aphid lineage to the parasitoid daughters (genotype effect). IR in the microcosm is the proportion of healthy aphids (non-mummified i.e. 33 unparasitoidised) after 11 days of exposure to the parasitoid genotype relative to the entire population of aphids (healthy and mummified) per aphid lineage. 34 The inner circle represents the absence of IGP (Without IGP) or its presence (With IGP), while the outer circle represents the parasitoid daughters including 35 the corresponding IR value (%) separated by a comma. There were 73 parasitoid daughters in total as the result of the quantitative genetic design (with IGP, n 36 = 30 parasitoid daughters, and without IGP, n = 43 parasitoid daughters). 37 38 39

6 40 41 Figure S3. Detailed Q1 immunity to parasitoid genotype with and without IGP. This ‘sunburst’ infographic illustrates the detailed immunity ratio (IR) in the 42 Q1 pea aphid lineage to the parasitoid daughters (genotype effect). IR in the microcosm is the proportion of healthy aphids (non-mummified i.e. unparasitoidised) 43 after 11 days of exposure to the parasitoid genotype relative to the entire population of aphids (healthy and mummified) per aphid lineage. The inner circle 44 represents the absence of IGP (Without IGP) or its presence (With IGP), while the outer circle represents the parasitoid daughters including the corresponding 45 IR value (%) separated by a comma. There were 45 parasitoid daughters in total as the result of the quantitative genetic design (with IGP, n = 30 parasitoid 46 daughters 30, and without IGP, n = 15 parasitoid daughters). 47

48 Molecular Analysis 49

50 Table 1. Primers used in this study. The table shows the target symbiont, the respective target genes with their primer names, followed by the sequence 51 (5’-3’) and the references given below the table.

Target symbiont Target gene Primer name Sequence (5’-3’) References*

Primers used in diagnostic 16s PCR H. defensa 16s rRNA 10F AGTTTGATCATGGCTCAGATTG 1

H. defensa 16s rRNA 16s- T419R AAATGGTATTSGCATTTATCG 1

Primers used in PCR reactions for 16s gene sequencing Universal 16s rRNA fD1 AGAGTTTGATCCTGGCTCAG 2

Universal 16s rRNA rP2 ACGGCTACCTTGTTACGACTT 2

52 * References: 1) Henry, L., Peccoud, J., Simon, J., Hadfield, J., Maiden, M., Ferrari, J. and , H., 2013. Horizontally transmitted symbionts and host colonization 53 of ecological niches. Current Biology, 23(17), pp.1713-1717. 2) Weisburg, W., Barns, S., Pelletier, D. and Lane, D., 1991. 16S ribosomal DNA amplification for 54 phylogenetic study. Journal of Bacteriology, 173(2), pp.697-703.

55 56 Gel extraction 57 Once the universal 16s PCR reactions were completed [95°C 5 mins, (95°C 30s, 60°C 30s, 72°C 30 sec) x 40 and a final extension of 72°C 7 mins] they were 58 visualised on a 1% agarose gel with SafeView Nucleic Acid Stain with Bioline HyperLadder™ 1kb. The gel bands were cut from the gel under UV light using a 59 sterile scalpel and extracted using the Qiagen QIAquick gel extraction kit.

60 Ligation and transformation 61 The ligation of the samples into a plasmid was conducted using the Promega ‘pGEM®-T Easy Vector System 1’ as per the manufacturer's protocol and set up 62 in the following reaction and left overnight at 4°C. 63 The transformation reaction used XL1-Blue competent cells (Agilent Technologies) and the manufactures ‘transformation protocol’ with some slight 64 alterations. We added 2 µl of the ligated plasmid sample to the aliquot of cells (Step 5). We also used LB media instead of the suggested SOC media (step 9) 65 and the LB-ampicillin plates were made by adding 15g of agar and 25g of LB medium to 1L of MiliQ water and mixing well. The LB agar was then autoclaved

66 and left to cool to below 55°C. When the mixture reached the correct temperature, it was taken to a laminar flow cabinet and the filter-sterilized ampicillin was 67 added (1 ml of a 100mg/ml solution). The agar is then mixed again and poured into Petri dishes and left to set in the laminar flow, under flame. For colour 68 screening, 100µl of 100 mM IPTG and 20µl of 50 mg/ml X-gal was pipetted onto the agar and spread evenly (step 10). The plates were left for 48 hours for the 69 colonies to develop as they were too small after the suggested 17 hours. After 48 hours of incubation at 37°C, the colonies were incubated at 4°C for 2 hours 70 to enhance the colours. The growth of any bacteria that did not take up the plasmid would be inhibited by the antibiotic and if a colony formed and contained 71 the plasmid but not the 16s gene we had tried to insert, then the colonies grow on the plates and appear blue in colour. Moreover, if the colonies contain the 72 plasmid with the 16s gene insert, they appear white, making the colonies easy to sample. The colonies were sampled by touching them with a pipette tip and 73 the tip was dropped into a falcon tube that contained 3ml of sterile LB-ampicillin media (same concentration of antibiotic as the plates). The sampled colonies 74 were incubated at 37°C, with shaking at 225-250 rpm, overnight. The next day the plasmids were extracted from the sampled colonies using the Qiagen, 75 QIAprep® spin miniprep kit and quick-start protocol and eluted with nuclease-free water.

76 Sample preparation for sequencing 77 Before the plasmids were sent for sequencing, 15 samples of each aphid line were digested with the restriction enzyme EcoR1. This enzyme removes the insert 78 sequence from the plasmid we had used and enabled us to visually confirm its presence before sequencing. After digestion, when the samples were run on a 79 1% agarose gel, they showed two clear bands, one for the plasmid and a band of a similar size to the insert. 80 To prepare the samples for sequencing their concentration was checked on a Thermo Scientific™ nanodrop™ 2000 spectrophotometer. GATC Biotech 81 AG, London UK, (now Eurofins GATC) recommended a DNA concentration of between 30 – 80 ng/µl for their plasmid ‘Supreme run’ Sanger sequencing. Those 82 samples that had a concentration higher than recommended were then diluted, with nuclease-free water, to a final concentration of 60ng/µl. A total of 70 83 samples (35 Q1 and 35 N116) were sequenced using GATC Biotech’s T7 sequencing primers.

84 Analysis of sequencing data 85 Sequences have been edited to remove pGEM-T Easy Vector sequences and any parts of the sequence that contained bases that did not pass the confidence 86 threshold.

87 N116 aphid symbiont BLAST analysis 88 Trimmed ‘N116’ clone Acyrthosiphon pisum symbiont 16s rRNA gene sequences used in the blast analysis shown in FASTA format. GenBank Accession 89 numbers: MW979375 to MW979398

90 > Seq1 [organism=Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 91 AGAGTTTGATCCTGGCTCAGATTGAACACTGGTGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGAGTGAGCGCAGTTTACTGAGTTCATGTCGGCG 92 AGCGGCGGACGGGTGAGTAAAGTCTGGGAATCTGGCCGAAGGAGGGGGATAACTGCTGGAAACGGCAGCTAATACCGCATGAAGTCGCGAGACCAAAG 93 TGGGGGACCTTCGGGCCTCACGCCTTCGGATGAGCCCAGATGAGATTAGCTGGTAGGTAAGGTAAGGGCTTACCTAGGCGACGATCTCTAGCGGGTCT 94 GAGAGGATAGCCCGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAG 95 CCATGCCACGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGCGATAAATGCGAATACCATTTATTTTTGACGTTACTCGCAG 96 AAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGATCGGAATAACTGGGCGTAAAGGGCATGTAGGCGGTGAG 97 TTAAGTCAGATGTGAAATCCCCGAGCTCAACTTGGGAATGGCATTTGAAACTGGGTCGCTAGAGTTTTCTAGAGGGGGGTAGAATTCCAGGTGTAGCGGT

98 GAAATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGAGAAAGACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTA 99 GATACCCTGGTAGTCCACGCTGTAAACGATGTCGATTTGGAGGTTGCGGTCTTGAACTGTGGCGTCCGGAGCTAACGCG 100 101 > Seq2 [organism=Candidatus Fukatsuia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 102 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGGGAAGGTAGCTTGCTATCTTTGCCGGCGAGC 103 GGCGGACGGGTGAGTAAAGTCTGGGGATCTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATGATGTTACGCGACCAAAGCGG 104 GGGACCTCCGGGCCTCGCGCCATCAGATGAACCCAGATGGGATTAGCTAGTAGGAGAGGTAATGGCTCCCCTAGGCGACGATCCCTAGCTGGTCTGAG 105 AGGATAACCAGCCACACTGGAACTGAGAGACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCA 106 TGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAATGAAGCAATGCAAAGAGTGTTGCTAATGGACGTTACTCGCAGAA 107 GAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCATAAAGGGCACGTAGGCGGTTTCTT 108 AAGTCAGATGTGAAATCCCCGAGCTTCACTTGGGAACGGCATTTGAAACTGAGAGTCTAGAGTTTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGA 109 AATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAGAGACTGACGCTGAGGTGCGAAAGCGTGGGTAGCAAACAG 110 111 > Seq3 [organism=Candidatus Fukatsuia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 112 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGGGAAGGTAGCTTGCTATCTTTGCCGGCGAGC 113 GGCGGACGGGTGAGTAAAGTCTGGGGATCTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATGATGTTACGCGACCAAAGCGG 114 GGGACCTCCGGGCCTCGCGCCATCAGATGAACCCAGATGGGATTAGCTAGTAGGAGAGGTAATGGCTCCCCTAGGCGACGATCCCTAGCTGGTCTGAG 115 AGGATAACCAGCCACACTGGAACTGAGAGACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCA 116 TGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAATGAAGCAATGCAAAGAGTGTTGCTAATGGACGTTACTCGCAGAA 117 GAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGAGCACGTAGGCGGTTTCTT 118 AAGTCAGATGTGAAATCCCCGAGCTTCACTTGGGAACGGCATTTGAAACTGAGAGTCTAGAGTTTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGA 119 AATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAGAGACTGACGCTGAGGTGCGAAAGCGTGGGTAGCAAACAGGATTAGA 120 TACCCTGGTAGTCCACGCTGTAAACGATGTCGATTTGTAGGTTGTGGTTATAAACTGTGGCTTGCGGAGCAAACGCGTTAAATCGACCGCCTGGGGAGTA 121 CGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCCACGCGAAGAACCTTACCTACTCT 122 TGACATCCAGAGG 123 124 > Seq4 [organism=Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 125 AGAGTTTGATCCTGGCTCAGGTTGAACACTGGTGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGAGTGAGCGCAGTTTACTGAGTTCATGTCGGCG 126 AGCGGCGGACGGGTGAGTAAAGTCTGGGAATCTGGCCGAAGGAGGGGGATAACTGCTGGAAACGGCAGCTAATACCGCATGAAGTCGCGAGACCAAAG 127 TGGGGGACCTTCGGGCCTCACGCCTTCGGATGAGCCCAGATGAGATTAGCTGGTAGGTAAGGTAAAGGCTTACCTAGGCGACGATCTCTAGCGGGTCT 128 GAGAGGATAGCCCGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAG 129 CCATGCCACGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGCGATAAATGCGAATACCATTTATTTTTGACGTTACTCGCAG 130 AAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGTAGGCGGTGAG 131 CTAAGTCAGATGTGAAATCCCCGAGCTCAACTTGGGAATGGCATTTGAAACTGGGTCGCTAGAGTTTTCTAGAGGGGGGTAGAATTCCAGGTGTAGCGGT 132 GAAATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGAGAAAGACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTA 133 GATACCCTGGTAGTCCACGCTGTAANCGATGTCGATTTGGAGGTTGCGGTCTTGAACTGTGGCGTCCGGAGCTAACGCGTTAAATCGACCGCCTGGGGA 134 GTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAA 135 136

137 > Seq5 [organism=Candidatus Fukatsuia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 138 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGGGAAGGTAGCTTGCTATCTTTGCCGGCGAGC 139 GGCGGACGGGTGAGTAAAGTCTGGGGATCTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATGATGTTACGCGACCAAAGCGG 140 GGGACCTCCGGGCCTCGCGCCATCAGATGAACCCAGATGGGATTAGCTAGTAGGAGAGGTAATGGCTCCCCTAGGCGACGATCCCTAGCTGGTCTGAG 141 AGGATAACCAGCCACACTGGAACTGAGAGACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCA 142 TGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAATGAAGCAATGCAAAGAGTGTTGCTAATGGACGTTACTCGCAGAA 143 GAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGCACGTAGGCGGCTTCTT 144 AAGTCAGATGTGAAATCCCCGAGCTTCACTTGGGAACGGCATTTGAAACTGAGAGTCTAGAGTTTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGA 145 AATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAGAGACTGACGCTGAGGTGCGAAAGCGTGGGTAGCAANCAGGATTAGA 146 TACCCTGGTAGTCCACGCTGTAAACGATGTCGATTTGTAGGTTGTGGTTATAAACTGTGGCTTGCG 147 148 > Seq6 [organism= Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 149 AGAGTTTGATCCTGGCTCAGATTGAACACTGGTGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGAGTGAGCGCAGTTTACTGAGTTCATGTCGGCG 150 AGCGGCGGACGGGTGAGTAAAGTCTGGGAATCTGGCCGAAGGAGGGGGATAACTGCTGGAAACGGCAGCTAATACCGCATGAAGTCGCGAGACCAAAG 151 TGGGGGACCTTCGGGCCTCACGCCTTCGGATGAGCCCAGATGAGATTAGCTGGTAGGTAAGGTAAAGGCTTACCTAGGCGACGATCTCTAGCGGGTCT 152 GAGAGGATAGCCCGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAG 153 CCATGCCACGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGCGATAAATGCGAATACCATTTATTTTTGACGTTACTCGCAG 154 AAGAAGCACTGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGTAGGCGGTGAG 155 TTAAGTCAGATGTGAAATCCCCGAGCTCAACTTGGGAATGGCATTTGAAACTGGGTCGCTAGAGTTTTCTAGAGGGGGGTAGAATTCCAGGTGTAGCGGT 156 GAAATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGAGAAAGACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTA 157 GATACCCTGGTAGTCCACGCTGTAAACGATGTCGATTTGGAGGTTGCGGTCTTGAACTGTGGCGTCCGGAGCTAACGCGTTAAATCGACCGCCTGGGGA 158 GTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGC 159 160 >Seq7 [organism=Candidatus Fukatsuia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 161 GGTTACCTTGTTACGACTTCACCCCAGTCATGGTTCACAAAGTGGTAAGCGCCATCCCAAAGGTTAAGCTACCTACTTCTTTTGCAAAACATTCCCATGGT 162 GTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACT 163 CCAATCCGGACTACGACGTACTTTATGAGGTCCGCTCACCCTCGCAGGCTCGCTTCTCTTTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAA 164 GGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTATCACCGGCAGTCTCTCTTGAGTTCCCACCTCTACGTGCTGGCAACAAAAGATAAGGG 165 TTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAAAGCTCCCCGAAGGGCACGTCAACATC 166 TCTGTCGACTCCTCTGGATGTCAAGAGTAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCG 167 168 169 170 171 172 173 174 175

176 > Seq9 [organism=Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 177 AGAGTTTGATCCTGGCTCAGATTGAACACTGGTGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGAGTGAGCGCAGTTTACTGAGTTCATGTCGGCG 178 AGCGGCGGACGGGTGAGTAAAGTCTGGGAATCTGGCCGAAGGAGGGGGATAACTGCTGGAAACGGCAGCTAATACCGCATGAAGTCGCGAGACCAAAG 179 TGGGGGACCTTCGGGCCTCACGCCTTCGGATGAGCCCAGATGAGATTAGCTGGTAGGTAAGGTAAAGGCTTACCTAGGCGACGATCTCTAGCGGGTCC 180 GAGAGGATAGCCCGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAG 181 CCATGCCACGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGCGATAAATGCGAATACCATTTATTTTTGACGTTACTCGCAG 182 AAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGTAGGCGGTGAG 183 TTAAGTCAGATGTGAAATCCCCGAGCTCAACTTGGGAATGGCATTTGAAACTGGGTCGCTAGAGTTTTCTAGAGGGGGGTAGAATTCCAGGTGTAGCGGT 184 GAAATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGAGAAAGACTGACGCTGAGGTGCGAAAGCGT 185 186 > Seq10 [organism=Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 187 AGAGTTTGATCCTGGCTCAGATTGAACACTGGTGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGTGTGAGCGCAGTTTACTGAGTTCATGTCGGCG 188 AGCGGCGGACGGGTGAGTAAAGTCTGGGAATCTGGCCGAAGGAGGGGGATAACTGCTGGAAACGGCAGCTAATACCGCATGAAGTCGCGAGACCAAAG 189 TGGGGGACCTTCGGGCCTCACGCCTTCGGATGAGCCCAGATGAGATTAGCTGGTAGGTAAGGTAAAGGCTTACCTAGGCGACGATCTCTAGCGGGTCT 190 GAGAGGATAGCCCGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAG 191 CCATGCCACGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGCGATAAATGCGAATACCATTTATTTTTGACGTTACTCGCAG 192 AAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGTAGGTGGTGAG 193 TTAAGTCAGATGTGAAATCCCCGAGCTCAACTTGGGAATGGCATTTGAAACTGGGTCGCTAGGGTTTTCTAGGGGGGGTAGAATTCCAGGTGTAGCGGT 194 GAAATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGAGAAAGACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTA 195 GATACCCTGGTAGTCCACGCTGTAAACGATGTCGATTTGGAGGTTGCGGTCTTGAACTGTGGCG 196 197 > Seq11 [organism=Candidatus Fukatsuia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 198 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGGGAAGGTAGTTTGCTATCTTTGCCGGCGAGCG 199 GCGGACGGGTGAGTAAAGTCTGGGGATCTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATGATGTTACGCGACCAAAGCGGG 200 GGACCTCCGGGCCTCGCGCCATCAGATGAACCCAGATGGGTTTAGCTAGTAGGAGAGGTAATGGCTCCCCTAGGCGACGATCCCTAGCTGGTCTGAGA 201 GGATAACCAGCCACACTGGAACTGAGAGACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCAT 202 GCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAATGAAGCAATGCAAAGAGTGTTGCTAATGGACGTTACTCGCAGAAG 203 AAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGAGCACGTAGGCGGTTTCTTAA 204 GTCAGACGTGAAATCCCCGAGCTTCACTTGGGAACGGCATTTGAAACTGAGAGTCTAGAGTTTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAA 205 ATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAGAGACTGACGCTGAGGTGCGAAAGCGTGGGTAGCAAACAGGATTAGAT 206 ACCCTGGTAGTCCACGCTGTAAACGATGTCGATTTGTA 207 208 209 210 211 212 213 214

215 > Seq12 [organism=Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 216 AGAGTTTGATCCTGGCTCAGATTGAACACTGGTGGCAGGCCTAACACATACAAGTCGAGCGGCATCGAGTGAGCGCAGTTTACTGAGTTCATGTCGGCG 217 AGCGGCGGACGGGTGAGTAAAGTCTGGGAATCTGGCCGAAGGAGGGGGATAACTGCTGGAAACGGCAGCTAATACCGCATGAAGTCGCGAGACCAAAG 218 TGGGGGACCTTCGGGCCTCACGCCTTCGGATGAGCCCAGATGAGATTAGCTGGTAGGTAAGGTAAAGGCTTACCTAGGCGACGATCTCTAGCGGGTCT 219 GAGAGGATAGCCCGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAG 220 CCATGCCACGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGCGATAAATGCGAATACCATTTATTTTTGACGTTACTCGCAG 221 AAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGTGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGTAGGCGGTGAG 222 TTAAGTCAGATGTGAAATCCCCGAGCTCAACTTGGGAATGGCATTTGAAACTGGGTCGCTAGAGTTTTCTAGAGGGGGGTAGAATTCCAGGTGTAGCGGT 223 GAAATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGAGAAAGACTGACGCTGAGGTGCGAAAGC 224 225 > Seq13 [organism=Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 226 AGAGTTTGATCCTGGCTCAGATTGAACACTGGTGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGAGTGAGCGCAGTTTACTGAGTTCATGTCGGCG 227 AGCGGCGGACGGGTGAGTAAAGTCTGGGAATCTGGCCGAAGGAGGGGGATAACTGCTGGAAACGGCAGCTAATACCGCATGAAGTCGCGAGACCAAAG 228 TGGGGGACCTTCGGGCCTCACGCCTTCGGATGAGCCCAGATGAGATTAGCTGGTAGGTAAGGTAAGGGCTTACCTAGGCGACGATCTCTAGCGGGTCT 229 GAGAGGATAGCCCGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAG 230 CCATGCCACGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGCGATAAATGCGAATACCATTTATTTTTGACGTTACTCGCAG 231 AAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGTAGGCGGTGAG 232 TTAAGTCAGATGTGAAATCCCCGAGCTCAACTTGGGAATGGCATTTGAAACTGGGTCGCTAGAGTTTTCTAGAGGGGGGTAGAATTCCAGGTGTAGCGGT 233 GAAATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGAGAAAGACTGACGCTGAGGTGCGAAAGCG 234 235 > Seq14 [organism=Candidatus Fukatsuia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 236 GGTTACCTTGTTACGACTTCACCCCAGTCATGGTTCACAAAGTGGTAAGCGCCATCCCAAAGGTTAAGCTACCTACTTCTTTTGCAAAACACTCCCATGGT 237 GTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACT 238 CCAATCCGGACTACGACGTACTTTATGAGGTCCGCTCACCCTCGCAGGCTCGCTTCTCTTTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAA 239 GGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTATCACCGGCAGTCTCTCTTGAGTTCCCACCTCTACGTGCTGGCAACAAAAGATAAGGG 240 TTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAAAGCTCCCCGAAGGGCACGTCAACATC 241 TCTGTCGACTCCTCTGGATGTCAAGAGTAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCAT 242 TTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTTGCTCCGCAAGCCACAGTTTATAACCACAACCTACAAATCGACATCGTTT 243 ACAGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTACCCTCGCTTTCGCACCTCAGCGTCAGTCTCTGTCCAGGGGGCCGCCTTCGCCACCGGTATT 244 CCTCCAGATATCTACGCATTTCACCGCTACACCTGGAAATTCTACCCCCCCTCTACAAAACTCT 245 246 247 248 249 250 251 252 253

254 > Seq15 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 255 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 256 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 257 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 258 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 259 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 260 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 261 TCAGATGTGAAATCCCCGCGCTCAACGTAGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAA 262 TGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGC 263 264 > Seq16 [organism=Candidatus Fukatsuia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 265 GGTTACCTTGTTACGACTTCACCCCAGTCATGGTTCACAAAGTGGTAAGCGCCATCCCAAAGGTTAAGCTACCTACTTCTTTTGCAAAACACTCCCATGGT 266 GTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACT 267 CCAATCCGGACTACGACGTACTTTATGAGGTCCGCTCACCCTCGCAGGCTCGCTTCTCTTTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAA 268 GGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTATCACCGGCAGTCTCTCTTGAGTTCCCACCTCTACGTGCTGGCAACAAAAGATAAGGG 269 TTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAAAGCTCCCCGAAGGGCACGTCAACATC 270 TCTGTCGACTCCTCTGGATGTCAAGAGTAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCAT 271 TTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTTGCTCCGCAAGCCACAGTTTATAACCACAACCTACAAATCGACATCGTTT 272 ACAGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTCTCTGTCCAGGGGGG 273 274 > Seq17 [organism=Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 275 AGAGTTTGATCCTGGCTCAGATTGAACACTGGTGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGAGTGAGCGCAGTTTACTGAGTTCATGTCGGCG 276 AGCGGCGGACGGGTGAGTAAAGTCTGGGAATCTGGCCGAAGGAGGGGGATAACTGCTGGAAACGGCAGCTAATACCGCATGAAGTCGCGAGACCAAAG 277 TGGGGGACCTTCGGGCCTCACGCCTTCGGATGAGCCCAGATGAGATTAGCTGGTAGGTAAGGTAAAGGCTTACCTAGGCGACGATCTCTAGCGGGTCT 278 GAGAGGATAGCCCGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAG 279 CCATGCCACGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGCGATAAATGCGAATACCATTTATTTTTGACGTTACTCGCAG 280 AAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGTAGGCGGTGAG 281 TTAAGTCAGATGTGAAATCCCCGAGCTCAACTTGGGAATGGCATTTGAAACTGGGTCGCTAGAGTTTTCTAGAGGGGGGTAGAATTCCAGGTGTAGCGGT 282 GAAATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGAGAAAGACTGACGCTGAGGTGCGAAAGC 283 284 > Seq18 [organism=Candidatus Fukatsuia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 285 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGCATCGGGAAGGTAGCTTGCTATCTTTGCCGGCGAGCG 286 GCGGACGGGTGAGTAAAGTCTGGGGATCTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATGATGTTACGCGACCAAAGCGGG 287 GGACCTCCGGGCCTCGCGCCATCAGATGAACCCAGATGGGATTAGCTAGTAGGAGAGGTAATGGTTCCCCTAGGCGACGATCCCTAGCTGGTCTGAGA 288 GGATAACCAGCCACACTGGAACTGAGAGACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCAT 289 GCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAATGAAGCAATGCAAAGAGTGTTGCTAATGGACGTTACTCGCAGAAG 290 AAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGCACGTAGGCGGTTTCTTA 291 AGTCAGATGTGAAATCCCCGAGCTTCACTTGGGAACGGCATTTGAAACTGAGAGTCTAGAGTTTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAA 292 ATGCGT

293 > Seq19 [organism=Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 294 AGAGTTTGATCCTGGCTCAGATTGAACACTGGTGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGAGTGAGCGCAGTTTACTGAGTTCATGTCGGCG 295 AGCGGCGGACGGGTGAGTAAAGTCTGGGAATCTGGCCGAAGGAGGGGGATAACTGCTGGAAACGGCAGCTAATACCGCATGAAGTCGCGAGACCAAAG 296 TGGGGGACCTTCGGGCCTCACGCCTTCGGATGAGCCCAGATGAGATTAGCTGGTAGGTAAGGTAAAGGCTTACCTAGGCGACGATCTCTAGCGGGTCT 297 GAGAGGATAGCCCGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAG 298 CCATGCCACGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGCGATAAATGCGAATACCATTTATTTTTGACGTTACTCGCAG 299 AAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGTAGGCGGTGAG 300 TTAAGTCAGATGTGAAATCCCCGAGCTCAACTTGGGAATGGCATTTGAAACTGGGTCGCTAGAGTTTTCTAGAGGGGGGTAGAATTCCAGGTGTAGCGGT 301 GAAATGCGTAGATATCTGGAGGAA 302 303 > Seq20 [organism=Buchnera aphidicola] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 304 GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCTTCCTTTTAAAGGGTTAGGATACCTGCTTCTTTTGCAACCCACTCCCA 305 TGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCGTGGAGTCGAGTTGCA 306 GACTCCatTCCGGACTACGATTTACTTTATGAGGTTTGCTTGTCTTTGCAGATTTGCTTCTCTTTGTATAAACCATTGTAGCACGTGTGTAGCCCTGGTCGTA 307 AGGGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGGTTTATAACCGGCAGTCTCCTCTGAGTTCCCGGCCGAACCGCTGGCAACAGGGGATAAGG 308 GTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCACAGCTCCCGAAGGCACTTCTTTATTTC 309 TAAAGAATTCTGTGGATGTCAAGACCAGGTAAGGTTTTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTTG 310 AGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAATGCGTTAGCTTCGGAAGTCACTTCTCTTGGAAACAACCTCCAAGTCGACATCGTTTAC 311 GGCATGGACTACCAGGGTATCTAATCCTGtTTGCTCCCCACGCtTTCGCGCCTCAGTGTC 312 313 > Seq21 [organism=Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 314 AGAGTTTGATCCTGGCTCAGATTGAACACTGGTGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGAGTGAGCGCAGTTTACTGAGTTCATGTCGGCG 315 AGCGGCGGACGGGTGAGTAAAGTCTGGGAATCTGGCCGAAGGAGGGGGATAACTGCTGGAAACGGCAGCTAATACCGCATGAAGTCGCGAGACCAGA 316 GTGGGGGACCTTTGGGCCTCACGCCTTCGGATGAGCCCAGATGTGATTAGCTGGTAGGTAAGGTAAAGGCTTACCTAGGCGACGATCTCTAGCGGGTCT 317 GAGAGGATAGCCCGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCCCAATGGGCGAAAGCCTGATGCAG 318 CCATGCCACGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGCGATAAATGCGAATACCATTTATTTTTGACGTTACTCGCAG 319 AAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGTAGGCGGTGAG 320 TTAAGCCAGATGTGAAATCCCCGAGCTCAACTTGGGAATGGCATTTGAAACTGGGTCGCTAGAGTTTTCTAGAGGGGGGTAGAATTCCAGGTGTAGCGGT 321 322 > Seq22 [organism=Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 323 AGAGTTTGATCCTGGCTCAGATTGAACACTGGTGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGAGTGAGCGCAGTTTACTGAGTTCATGTCGGCG 324 AGCGGCGGACGGGTGAGTAAAGTCTGGGAATCTGGCCGAAGGAGGGGGATAACTGCTGGAAACGGCAGCTAATACCGCATGAAGTCGCGAGACCAAAG 325 TGGGGGACCTTCGGGCCTCACGCCTTCGGATGAGCCCAGATGAGATTAGCTGGTAGGTAAGGTAAAGGCTTACCTAGGCGACGATCTCTAGCGGGTCT 326 GAGAGGATAGCCCGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAG 327 CCATGCCACGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGCGATAAATGCGAATACCATTTATTTTTGACGTTACTCGCAG 328 AAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGTAGGCGGTGAG 329 TTAAGTCAGATGTGAAATCCCCGAGCTCAACTTGGGAATGGCATTTGAAACTGGGTCGCTAGAGTTTTCTAGAGGGGGGTAGAATTCCAGGTGTAGCGGT 330 GAAATGCGTAGATATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGAGAAAGACTGACGCTGAGG 331

332 > Seq23 [organism=Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 333 GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCTTGCGGTTTAGCTACCTACTTCTTTTGCAACCCACTCCCATGGT 334 GTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGCATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACT 335 CCAATCCGGACTACGACATACTTTCTGAGTTCCGCTTTCCCTCGCAGGTTCGCATCCCTTTGTATACGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAG 336 GGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGGTTTATCACCGGCAGTCTCCTTTGAGTTCCCGCCTCTACGCGCTGGCAACAAAGGACAAGGG 337 ATGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCACGGTTCCCGAAGGCACTTGCGCATCTC 338 TGCACAATTCCGTGGATGTCAAGAGTAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTT 339 GAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGATTTAACGCGTTAGCTCCGGACGCCACAGTTCAAGACCGCAACCTCCAAATCGACATCGTTTA 340 CAGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTCTTTCTCCAGGGGGCCGCCTTCGCCACCGGTATTC 341 CTCCAGATATCTACGCATTTCACCGCTACACCT 342 343 > Seq24 [organism=Candidatus Fukatsuia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 344 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGGGAAGGTAGCTTGCTATCTTTGCCGGCGAGC 345 GGCGGACGGGTGAGTAAAGTCTGGGGATCTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATGATGTTACGCGACCAAAGCGG 346 GGGACCTCCGGGCCTCGCGCCATCAGATGAACCCAGATGGGATTAGCTAGTAGGAGAGGTAATGGCTCCCCTAGGCGACGATCCCTAGCTGGTCTGAG 347 AGGATAACCAGCCACACTGGAACTGAGAGACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCA 348 TGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAATGAAGCAATGCAAAGAGTGTTGCTAATGGACGTTACTCGCAGAA 349 GAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGCACGTAGGCGGTTTCTT 350 AAGTCAGATGTGAAATCCCCGAGCTTCACTTGGGAACGGCATTTGAAACTGAGAGTCTAGAGTTTTGTAGAGGGGGGTAGAAT 351 352 > Seq25 [organism=Candidatus Hamiltonella defensa] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate N116] 353 AGAGTTTGATCCTGGCTCAGATTGAACACTGGTGGCAGGCCTAACACATGCAAGTCGAGCGGCATCGAGTGAGCGCAGTTTACTGAGTTCATGTCGGCG 354 AGCGGCGGACGGGTGAGTAAAGTCTGGGAATCTGGCCGAAGGAGGGGGATAACTGCTGGAAACGGCAGCTAATACCGCATGAAGTCGCGAGACCAAAG 355 TGGGGGACCTTCGGGCCTCACGCCTTCGGATGAGCCCAGATGAGATTAGCTGGTAGGTAAGGTAAAGGCTTACCTAGGCGACGATCTCTAGCGGGTCT 356 GAGAGGATAGCCCGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAG 357 CCATGCCACGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGCGATAAATGCGAATACCATTTATTTTTGACGTTACTCGCAG 358 AAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGTAGGCGGTGAG 359 TTAAGTCAGATGTGAAATCCCCGAGCTCAACTTGGGAATGGCATTTGAAACTGGGTCGCTAGAGTTTTCTAGAGGGGGGTAGAATTCCAGGTGT 360 361 362 Q1 aphid symbiont BLAST analysis 363 Trimmed ‘Q1 clone Acyrthosiphon pisum symbiont 16s rRNA gene sequences used in the blast analysis shown in FASTA format. GenBank Accession numbers: 364 MW971996 to MW972018

365 > Seq1 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 366 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 367 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 368 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG

369 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 370 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 371 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 372 TCAGATGTGAAATCCCCGCGCTCAACGTAGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGG 373 374 > Seq2 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 375 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 376 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 377 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTAGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGCCTGAGAG 378 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 379 CCGCGTGTGTGAAGAGGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 380 GCACCGGCTAACTCCGTGCCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 381 TCAGATGTGAAATCCCCGCGCTCAACGTGGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGGGTAGAATTC 382 383 > Seq3 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 384 CGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCT 385 GATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGGGACCTTCGGGCCTCACGCCATCAGATGTGCCC 386 AGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGT 387 CCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAA 388 AGCAC 389 390 > Seq4 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 391 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 392 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 393 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTAGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 394 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 395 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 396 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 397 TCAGATGTGAAATCCCCGCGCTCAACGTGGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGGGTAGAATT 398 399 > Seq5 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 400 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 401 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 402 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 403 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCAGG 404 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 405 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 406 TCAGATGTGAAATCCCCGCGCTCAACGTAGGAACGGCATTTGAGACTGGCAA 407

408 > Seq6 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 409 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 410 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 411 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 412 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 413 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 414 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 415 TCAGATGTGAAATCCCCGCGCTCAACGTGGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAA 416 TGCG 417 418 > Seq7 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 419 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 420 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 421 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 422 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 423 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 424 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGG 425 426 > Seq8 [organism= Buchnera aphidicola] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 427 GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCTTCCTTTTAAAGGGTTAGGATACCTGCTTCTTTTGCAACCCACTCCCA 428 TGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCGTGGAGTCGAGTTGCA 429 GACTCCAGTCCGGACTACGATTTACTTTATGAGGTTTGCTTGTCTTTGCAGATTTGCTTCTCTTTGTATAAACCATTGTAGCACGTGTGTAGCCCTGGTCGT 430 AAGGGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGGTTTATAACCGGCAGTCTCCTCTGAGTTCCCGGCCGAACCGCTGGCAACAGGGGATAAG 431 GGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCACAGCTCCCGAAGGCACTTCTTTATTT 432 CTAAAGAATTCTGTGGATGTCAAGACCAGGTAAGGTTTTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTT 433 GAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAATGCGTTAGCTTCGGAAGTCACTTCTCTTGGAAACAACCTCCAAGTCGACATCGTTTA 434 CGGCATGGACCACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTTTT 435 436 > Seq9 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 437 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 438 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 439 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTAGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 440 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 441 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 442 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 443 TCAGATGTGAAATCCCCGCGCTCAACGTGGGAACGGCATTTTGAGACTG 444 445 446

447 > Seq10 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 448 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCAG 449 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 450 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 451 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 452 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 453 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 454 TCAGATGTGAAATCCCCGCGCTCAACGTGGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGG 455 456 > Seq11 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 457 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 458 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 459 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 460 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 461 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 462 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 463 TCAGATGTGAAATCCCCGCGCTCAACGT 464 465 > Seq12 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 466 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 467 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 468 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 469 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 470 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 471 GCATCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGTTTGTTAAGT 472 CAGATGTGAAATCCCCGCGCTCAACGTGGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAAT 473 GCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATAC 474 CCTGGTAGTCCACGCTGTAAACGATGTCGATTTGGAGGTTGCGCCCTTGAGGGGTGGCTTCCGTAGCTAACGCGTTAAATCGACCGCCTGGGGGAGTAC 475 G 476 477 478 479 480 481 482 483 484 485

486 > Seq13 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 487 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 488 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 489 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTAGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 490 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 491 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 492 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 493 TCAGATGTGAAATCCCCGCGCTCAACGTGGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAA 494 TGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATA 495 CCCTGGTAGTCCACGCTGTAAACGATGTCGATTTGGAGGTTGCGCCCCTTG 496 497 > Seq14 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 498 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCCTGCTCTCTGGGTGACGAGCGG 499 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 500 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTAGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 501 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 502 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 503 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 504 TCAGATGTGAAATCCCCGCGCTCAACGTGGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAA 505 TGCGTAGAGATCTGGAGGA 506 507 > Seq15 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 508 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 509 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 510 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCCGGTCTGAGAG 511 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 512 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 513 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 514 TCAGATGTGAAATCCCCGCGCTCAACGTAGGAACGGCATTTGAGACTGGCAA 515 516 > Seq16 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 517 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 518 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 519 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 520 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAAAGGGCGCAAGCCTGATGCAGCCATG 521 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCA 522 523 524

525 > Seq17 [organism= Buchnera aphidicola] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 526 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAAGCCTAACACATGCAAGTCGAGCGGCAGCGAGAAGAGAGCTTGCTCTCTTTGTCGGCAAGCG 527 GCAAACGGGTGAGTAATATCTGGGGATCTACCCAAAAGAGGGGGATAACTACTAGAAATGGTAGCTAATACCGCATAATGTTGAAAAACCAAAGTGGGGG 528 ACCTTTTGGCCTCATGCTTTTGGATGAACCCAGACGAGATTAGCTTGTTGGTAGAGTAATAGCCTACCAAGGCAACGATCTCTAGCTGGTCTGAGAGGAT 529 AACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCTATGCCG 530 CGTGTATGAAGAAGGCCTTAGGGTTGTAAAGTACTTTCAGCGGGGAGGAAAAAAATAAAACTAATAATTTTATTTCGTGACGTTACCCGCAGAAGGAGCAC 531 CGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCAGAATTACTGGGCGTAAAGAGCGCGTAGGTGGTTTTTTAAGTCAGG 532 TGTGAAATCCCTAGGCTCAACCTAGGAACTGCATTTGAAACTGGAAAACTAGAGTTTCGTAGAGGGAGGTAGAATTCTAGGTGTAGCGGTGAAATGCGTA 533 GATATCTGGAGGAATACCCGTGGCGAAAGCGGCCTCCTAAACGAAAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGG 534 TAGTCCATGCCGTAAACGATGTCGACTTGGAGGTTGTTTCCAAGAGAAGTGACTTCCGAAGCTAACGCATTAAGTCGACCGCCTGGGGGAGTACGGCCG 535 CAAGGCTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGC 536 537 > Seq18 [organism= Buchnera aphidicola] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 538 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAAGCCTAACACATGCAAGTCGAGCGGCAGCGAGAAGAGAGCTTGCTCTCTTTGTCGGCAAGCG 539 GCAAACGGGTGAGTAATATCTGGGGATCTACCCAAAAGAGGGGGATAACTACTAGAAATGGTAGCTAATACCGCATAATGTTGAAAAACCAAAGTGGGGG 540 ACCTTTTGGCCTCATGCTTTTGGATGAACCCAGACGAGATTAGCTTGTTGGTAGAGTAATAGCCTACCAAGGCAACGATCTCTAGCTGGTCTCAGAGGATA 541 ACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCTATGCCGC 542 GTGTATGAAGAAGGCCTTAGGGTTGTAAAGTACTTTCAGCGGGGAGGAAAAAAATAAAACTAATAATTTTATTTCGTGACGTTACCCGCAGAAGAAGCACC 543 GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCAGAATTACTGGGCGTAAAGAGCGCGTAGGTGGTTTTTTAAGTCAGGT 544 GTGAAATCCCTAGGCTCAACCTAGGAACTGCACTTGAAACTGGAAAACTAGAGTTTCGTAGAGGGAGGTAGAATTCTAGGTGTAGCGGTGAAATGCGTAG 545 ATATCTGGAGGAATACCCGTGGCGAAAGCGGCCTCCTAAACGAAAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAG 546 547 > Seq19 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 548 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 549 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCGTAACGTCGCAAGACCAAAGTGGGG 550 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 551 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 552 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 553 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 554 TCAGATGTGAAATCCCCGCGCTCAACGTGGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGCAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAA 555 TGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCAGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATA 556 CCCTGGTAGTCCACGCTGTAAACGATGTCGATTTGGAGGTTGCGCCCTTGAGGGGTGGCTTCCGTAGCTAACGCGTTAAATCGACCGCCTGGGGAGTAC 557 GGCCGcAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCG 558 559 560 561 562 563

564 > Seq20 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 565 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 566 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGG 567 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTAGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 568 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCGAGCCTGATGCAGCCATG 569 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 570 GCACCGGCTAGCTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 571 TCAGATGTGAAATCCCCGCGCTCAACGTGGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAA 572 TGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATA 573 CCCTGGTAGTCCACGCTGTAAACGATGTCGATTTGGAGGTTGCGCCCTTGAGGGGTGGCTTCCGTAGCTAACGCGTTAAATCGACCGCC 574 575 > Seq21 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 576 AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGG 577 CGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACATCGCAAGACCAAAGTGGGG 578 GACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTAGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAG 579 GATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 580 CCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAA 581 GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAG 582 TCAGATGTGAAATCCCCGCGCTCAACGTGGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAA 583 TGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATA 584 CCCTGGTAGTCCACGCTGTAAACGATGTCGATTTGGAGGTTGCGCCCTTGAGGGGTGGCTTCCGTAGCTAACGCGTTAAATCGACCGCCTGGGGAGTAC 585 GGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGC 586 587 > Seq22 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 588 AGAGTTTGATCCTGGCTCAGATTgaACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGGC 589 GGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGGG 590 ACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTGGGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGG 591 ATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGC 592 CGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTACAGCGAGAAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAAG 593 CACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT 594 CAGATGTGAAATCCCCGCGCTCAACGTAGGAACGGCATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGCGTAGCGGTGAAAT 595 GCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATAC 596 CCTGGTAGTCCACGCTGTAAACGATGTCGATTTGGAGGTTGCGCCCTTGAGGGGTGGCTTCCGTAGCTAACGCGTTAAATCGACCGCCTGGGGAGTACG 597 GCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGaGCATGTGGTTTAATTCGATGCAACGCGA 598 599 600 601 602

603 > Seq23 [organism= Candidatus Serratia symbiotica] 16S ribosomal RNA gene [host=Acyrthosiphon pisum isolate Q1] 604 GGCCTAACACATGCAAGTCGAGCGGTAGCACAAGAGAGCTTGCTCTCTGGGTGACGAGCGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGG 605 CGGGGGATAACTAGTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGTGGGGGACCTTCGGGCCTCACGCCATCAGATGTGCCCAGGTA 606 GGATTAGCTGGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGA 607 CTCCTACGGGAGGCAGCAGCGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCAC 608 CTTCAGCGAGGAGAAAGGGTAATGTGTTAATAAGACATTGCATTGACGTTACTCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATA 609 CGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGCGCTCAACGGGGGAACGG 610 CATTTGAGACTGGCAAGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGG 611 CGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGATGTCGATTTGG 612 AGGTTGCGCCCTTGAGGGGTGGCTTCCGTAGCTAACGCGTTAAATCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGG 613 GCCCGCACAAGCGGTGGAGCATGT 614 615 616 617

618 Table S1. Summary of the statistical model of aphid immunity. Analysis of Deviance Table (Type II Wald chi-square tests) of the mixed effect generalised 619 linear model (Model1) investigating the effects of intraguild predation by the aphid lion (No [without IGP], Yes [with IGP]), pea aphid lineage (N116, Q1), 620 parasitoid genotype and the two-way interactions between the main effects on aphid immunity ratio (IR). Resulting from a full quantitative genetic mating design, 621 with n sires being mated to at least three dams, the sire × dam mating groupings produced the intraspecific genetic variability in the parasitoid 622 (genotype/daughters [sibs, and half-sibs]). IR in the microcosm is the proportion of healthy aphids (non-mummified i.e. unparasitoidised) after 11 days of 623 exposure to the parasitoid genotype relative to the entire population of aphids (healthy and mummified) per aphid lineage. Significant results are shown in bold. 624 χ2 Df Pr(>Chisq) IGP 1.74 1 0.188 Aphid Lineage 28.5 1 < 0.0001 Parasitoid Genotype 95.76 37 < 0.0001 Aphid Lineage x Parasitoid Genotype 12.1 3 0.007 IGP x Parasitoid Genotype 37.23 15 0.001 IGP x Aphid Lineage 2.03 1 0.155 625 626 627 Table S2. Summary of the statistical model of aphid mummy position. Analysis of Deviance Table (Type II tests) of the generalised linear model (Model2, 628 specified in the main text) applied to investigate the effects of intraguild predation by aphid lion (No [without IGP], Yes [with IGP]), pea aphid lineage (N116, 629 Q1), parasitoid genotype and the presented interactions between the main effects on the proportions of mummified aphid off the host plant at the 11th day of 630 the experiment. Resulting from a full quantitative genetic mating design, with n sires being mated to at least three dams, the sire × dam mating groupings 631 produced the intraspecific genetic variability in the parasitoid (genotype/daughters [sibs, and half-sibs]). Significant results are shown in bold, marginally 632 significant results are shown in italics. 633

2 LRχ Df Pr(>Chisq) IGP 0.29 1 0.59 Aphid Lineage 7.05 1 0.008 Parasitoid Genotype 40.62 28 0.058 Aphid Lineage x Parasitoid Genotype 4.373 2 0.112 634