Product Information Sheet for NR-20721

Monoclonal Anti-Human NLRP4 (NALP4), be stored at -20°C or colder immediately upon arrival. Freeze-thaw cycles should be avoided. Clone U54.M.hNALP4.8 (produced in vitro) Functional Activity: Catalog No. NR-20721 NR-20721 reacts with human NLRP4 in ELISA and western blot assays. See Certificate of Analysis for details. The For research use only. Not for human use. antibody is also reported to function in immunoprecipitation.1

Contributor: Citation: Richard J. Ulevitch, Ph.D., and Luc Teyton, M.D., Ph.D., Acknowledgment for publications should read “The following Department of Immunology and Microbial Science, The reagent was obtained through BEI Resources, NIAID, NIH: Scripps Research Institute, La Jolla, CA, USA` Monoclonal Anti-Human NLRP4 (NALP4), Clone U54.M.hNALP4.8 (produced in vitro), NR-20721.” Manufacturer: BEI Resources Biosafety Level: 1 Appropriate safety procedures should always be used with Product Description: this material. Laboratory safety is discussed in the following Antibody Class: IgG1κ publication: U.S. Department of Health and Human Services, Mouse monoclonal antibody prepared against the human Public Health Service, Centers for Disease Control and NLRP4 protein (also known as NALP4) was purified from Prevention, and National Institutes of Health. Biosafety in clone U54.M.hNALP4.8 hybridoma supernatant by protein G Microbiological and Biomedical Laboratories. 5th ed. affinity chromatography. The B cell hybridoma was Washington, DC: U.S. Government Printing Office, 2009; see generated by the fusion of P3X63Ag8.653 mouse myeloma www.cdc.gov/biosafety/publications/bmbl5/index.htm. cells with splenocytes from mice immunized by intraperitoneal injection with a truncated recombinant form of Disclaimers: the human NLRP4 protein containing the pyrin domain.1 You are authorized to use this product for research use only. It is not intended for human use. NLRP4 is encoded by the NLRP4 (nucleotide-binding domain and leucine rich repeat containing family, pyrin domain Use of this product is subject to the terms and conditions of containing 4) gene. NLRP proteins are characterized by an the BEI Resources Material Transfer Agreement (MTA). The N-terminal pyrin domain, a central NTPase domain and a C- MTA is available on our Web site at www.beiresources.org. terminal leucine-rich repeat region. NLR proteins are localized to the cytoplasm and recognize microbial products, While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, and their activation results in inflammatory responses ® mediated by NF-κB, MAP kinase, or caspase activation.2 neither ATCC nor the U.S. Government makes any Certain NLRPs are involved in the formation and activation of warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for inflammasomes, large cytoplasmic multiprotein complexes ® that in turn recruit and activate caspase-1.3,4 Activated informational purposes only. Neither ATCC nor the U.S. caspase-1 cleaves the pro-forms of the interleukin-1 cytokine Government warrants that such information has been family members leading to the secretion of active IL-1 family confirmed to be accurate. cytokines, which have multiple pro-infammatory activities. Inflammasomes are an important component of the innate This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. immune system. A variety of names (including NALP) have ® been used to describe the products encoded by the NLR ATCC and the U.S. Government are not liable for any gene family. A standardized nomenclature for the family was damages or injuries arising from receipt and/or use of this recently proposed.5 product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. ® Material Provided: Government, ATCC , their suppliers and contributors to BEI Each vial of NR-20721 contains approximately 100 µL of Resources are not liable for damages arising from the purified monoclonal antibody in PBS. The concentration, misidentification or misrepresentation of products. expressed as mg per mL, is shown on the Certificate of Analysis. Use Restrictions: This material is distributed for internal research, non- Packaging/Storage: commercial purposes only. This material, its product or its NR-20721 was packaged aseptically in screw-capped plastic derivatives may not be distributed to third parties. Except as vials and is provided frozen on dry ice. The product should performed under a U.S. Government contract, individuals contemplating commercial use of the material, its products or

BEI Resources E-mail: [email protected] www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898

Product Information Sheet for NR-20721

its derivatives must contact the contributor to determine if a license is required. U.S. Government contractors may need a license before first commercial sale.

References: 1. R. J. Ulevitch and L. Teyton, personal communication. 2. Wilmanski, J. M., T. Petnicki-Ocwieja, and K. S. Kobayashi. “NLR Proteins: Integral Members of Innate Immunity and Mediators of Inflammatory Diseases.” J. Leukoc. Biol. 83 (2008): 13-30. PubMed: 17875812. 3. Latz, E. “The Inflammasomes: Mechanisms of Activation and Function.” Curr. Opin. Immunol. 22 (2010): 28-33. PubMed: 20060699. 4. Skeldon, A., and M. Saleh. “The Inflammasomes: Molecular Effectors of Host Resistance Against Bacterial, Viral, Parasitic, and Fungal Infections.” Front. Microbiol. 2 (2011): 15. PubMed: 21716947. 5. Ting, J. P., et al. “The NLR Gene Family: A Standard Nomenclature.” Immunity 28 (2008): 285-287. PubMed: 18341998.

ATCC® is a trademark of the American Type Culture Collection.

BEI Resources E-mail: [email protected] www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898