16 CFR Ch. I (1–1–20 Edition) § 305.22
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Your Fluorescence Microscope Transmitted-Light
Your Fluorescence Microscope Transmitted-light. Bright-field Bright-field microscopy = Transmitted-light INVERTED UPRIGHT Fluorescence microscopy = Reflected-light Mercury Lamp Heat Filter Emission Filter Mirror Excitation Filter Collimating Lens Dichromatic Mirror (From:http://micro.magnet.fsu.edu) You need to know … Your light source Your filters Your objective Your detector Spectrum of a Mercury Lamp Your Light Source • Mercury lamp Wavelength (nm) • Xenon lamp Spectrum of a Xenon Lamp • Metal halide lamp • Halogen lamp • LED • Laser Wavelength (nm) (Modified from: h6p://www.cairn-research.co.uk) Your Light Source 1) Halogen lamp 2) Mercury lamp 3) Xenon lamp 4) Metal halide lamp 5) LED 6) Laser Tungsten – Halogen lamp • White light source • Inexpensive long lasNng bulbs • Used mainly for brighQield illuminaNon • CAN be used for fluorescence excitaNon above 400nm • Ideal for live cell imaging (low power, no UV) • “Colour” changes with temperature Mercury (HBO) lamp PROS • white light source • 10-100x brighter then halogen • focused intensity light-source • very bright intensity peaks at specific wavelengths for many standard fluoreophores CONS • short bulb life (≈200-400h) • generates a lot of heat • requires bulb alignment • no uniform intensity (peaks) • bulb are hazardous waste • long warm-up time • excitation wavelength cannot be • Intensity decay over Nme, intensity controlled independently flickering Xenon lamp PROS • white light source • relaNvely even intensity across visible spectrum • focused intense light source CONS • requires bulb alignment • bulbs are hazardous waste • Intensity decay over Nme • weaker intensity in UV • generates a lot of heat in the IR region • relaNvely low power in visible range • excitaNon wavelength cannot be controlled independently Metal Halide lamp PROS • white light source • brighter intensity between peaks than mercury lamp • no bulb alignment, more uniform field of illum. -
Fluorescence Cell Imaging and Manipulation Using Conventional Halogen Lamp Microscopy
Fluorescence Cell Imaging and Manipulation Using Conventional Halogen Lamp Microscopy Kazuo Yamagata1,2, Daisaku Iwamoto3, Yukari Terashita1,4, Chong Li1, Sayaka Wakayama1,Yoko Hayashi-Takanaka5, Hiroshi Kimura5, Kazuhiro Saeki3, Teruhiko Wakayama1* 1 RIKEN Center for Developmental Biology, Kobe, Japan, 2 Research Institute for Microbial Research, Osaka University, Suita, Japan, 3 Department of Genetic Engineering, Kinki University, Kinokawa, Wakayama, Japan, 4 Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan, 5 Graduate School of Frontier Biosciences, Osaka University, Suita, Japan Abstract Technologies for vitally labeling cells with fluorescent dyes have advanced remarkably. However, to excite fluorescent dyes currently requires powerful illumination, which can cause phototoxic damage to the cells and increases the cost of microscopy. We have developed a filter system to excite fluorescent dyes using a conventional transmission microscope equipped with a halogen lamp. This method allows us to observe previously invisible cell organelles, such as the metaphase spindle of oocytes, without causing phototoxicity. Cells remain healthy even after intensive manipulation under fluorescence observation, such as during bovine, porcine and mouse somatic cell cloning using nuclear transfer. This method does not require expensive epifluorescence equipment and so could help to reduce the science gap between developed and developing countries. Citation: Yamagata K, Iwamoto D, Terashita Y, Li C, Wakayama S, et al. (2012) Fluorescence Cell Imaging and Manipulation Using Conventional Halogen Lamp Microscopy. PLoS ONE 7(2): e31638. doi:10.1371/journal.pone.0031638 Editor: Sue Cotterill, St. Georges University of London, United Kingdom Received September 12, 2011; Accepted January 10, 2012; Published February 8, 2012 Copyright: ß 2012 Yamagata et al. -
Design & Fabrication of a Gravity Powered Light
See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/286194982 Design & Fabrication Of A Gravity Powered Light Conference Paper · November 2015 DOI: 10.13140/RG.2.2.28749.03049 CITATIONS READS 2 32,543 3 authors, including: Sabir Hossain Kunsan National University 22 PUBLICATIONS 41 CITATIONS SEE PROFILE Some of the authors of this publication are also working on these related projects: Autonomous Vehicle View project All content following this page was uploaded by Sabir Hossain on 11 December 2015. The user has requested enhancement of the downloaded file. Proceedings of the International Conference on Mechanical Engineering and Renewable Energy 2015 (ICMERE2015) 26 – 29 November, 2015, Chittagong, Bangladesh ICMERE2015-PI-254 DESIGN AND FABRICATION OF A GRAVITY POWERED LIGHT Md. Shahed Hossen1, Md. Tazul Islam2,* and Sabir Hossain3 1-3Dept. of Mechanical Engineering, CUET, Bangladesh [email protected],2,*[email protected],[email protected] Abstract- In recent times due to effects of pollution and global warming there is a need for generating power from renewable sources. The reason for generating power using gravity is that it is available all over the Earth, abundant and consistent too. In this project, the gravitational energy of a heavy particle is converted to the electrical energy. When the heavy particle falls down from a higher altitude to a lower one, its potential energy is converted into the kinetic energy. Then this energy is converted to electricity by using a synchronous motor. With the increasing of the altitude of the load, the lighting time increases. If load increases, power production also increases, but the lighting time decreases. -
1 Industrial Property Registrations Directorate IP Online Journal 22/2020
Industrial Property Registrations Directorate IP Online Journal 22/2020 - 25 May, 2020 Trademarks Publication for Oppositions In accordance with Rule 5 of the Trademark Search and Opposition Rules, 2018, the following applications for a trademark are being published. Opposition to the registration of the trademarks listed hereunder may be submitted to the Commerce Department within sixty (60) working days following the date of this publication via the ‘Notice of Opposition to a New Trademark Application’ section that can be found on the IP Portal www.ips.gov.mt, together with the prescribed fee of €50.00. (210) TM/ 59755 (731) Applicant: (220) Filing Date: 26-06-2019 Integrated Talent Ventures Limited (C47130) Capital Business Centre SGN 3000 San Gwann. Malta (540) Representation of the mark jobsinmalta.com The representation and full details of the mark are found on the National Trademark Register at: https://ips.gov.mt/NR/TM59755 (511) Class number: 9 (510) Goods/Services: Computer Software (526) Disclaimer: Registration gives no right to the exclusive use of: a) the words ‘jobs’, ‘in’ and ‘malta’ except when used together, and in the sequence as shown on the mark, and; b) the symbol and word ‘.com’. (210) TM/ 60353 (731) Applicant: (220) Filing Date: 08-12-2019 Moufid Abedalbaki 11, Sean Court, Flat 12 SPB 1147 Qawra. Poland (540) Representation of the mark 1 The representation and full details of the mark are found on the National Trademark Register at: https://ips.gov.mt/NR/TM60353 (531) Vienna Classification: 07.01.08, 07.03.02, 26.02.0126.02.07 (511) Class number: 1 (510) Goods/Services: Additives for use in mixing cement; Aeration chemicals for concrete (526) Disclaimer: Registration gives no right to the exclusive use of i) the word D-LUX except as distinctively reproduced ii) the word ‘Projects’ . -
Federal Register/Vol. 86, No. 158/Thursday, August 19, 2021
Federal Register / Vol. 86, No. 158 / Thursday, August 19, 2021 / Proposed Rules 46611 was associated with the official agency’s support the identified reason for the DEPARTMENT OF ENERGY inability to provide service in 6 hours or challenge. 10 CFR Part 430 less, or timely issuance of the results (C) Determination. The Service will and certificate, the Service may not consider impacts on the assigned [EERE–2021–BT–STD–0012] terminate the exception. If the Service official agency, the applicant, and the terminates a long-term exception, all RIN 1904–AF22 potential gaining agency when deciding parties will be notified, and the whether to grant an exception. These Energy Conservation Program: applicant will resume service with the Definitions for General Service Lamps assigned official agency within 60 days impacts may include, but are not limited to, the viability of the assigned of notification. AGENCY: Office of Energy Efficiency and official agency given the loss of (2) Nonuse of service exception. If an Renewable Energy, Department of business. The Service will also consider applicant has not received service from Energy. the impact on the integrity of the official the assigned official agency within the ACTION: Notice of proposed rulemaking system and confirm an exception would last 90 days, the applicant may request and announcement of public meeting. that the Service grant a nonuse of not undermine the congressional service exception. policies in section 2 of the United States SUMMARY: On January 19, 2017, the U.S. (i) Requests must clearly state and Grain Standards Act. The Service will Department of Energy (‘‘DOE’’) support the following: provide its decision in writing to the published two final rules adopting (A) The last date of service from assigned official agency, the applicant, revised definitions of general service assigned official agency; and the potential gaining agency. -
Flashlight Ebook
FLASHLIGHT PDF, EPUB, EBOOK Lizi Boyd | 40 pages | 12 Aug 2014 | CHRONICLE BOOKS | 9781452118949 | English | California, United States Flashlight PDF Book App Store Preview. The source of the light often used to be an incandescent light bulb lamp but has been gradually replaced by light-emitting diodes LEDs since the mids. Some models of flashlight include an acceleration sensor to allow them to respond to shaking, or to select modes based on what direction the light is held when switched on. LED flashlights were made in the early s. Perf Power. This was the first battery suitable for portable electrical devices, as it did not spill or break easily and worked in any orientation. CS1 maint: archived copy as title link U. Water resistance, if specified, is evaluated after impact testing; no water is to be visible inside the unit and it must remain functional. The standard described only incandescent lamp flashlights and was withdrawn in Colored light is occasionally useful for hunters tracking wounded game after dusk, or for forensic examination of an area. Solar powered flashlights use energy from a solar cell to charge an on-board battery for later use. Remove All. Don't feel overwhelmed with our surplus of options. Retailer Walmart. Anodized Aluminum. A flashlight may have a red LED intended to preserve dark adaptation of vision. Price Free. And it even goes with a compass, giving you the direction in the darkness. Lanterns Lanterns. The working distance is from the point of view of the user of the flashlight. An IP X8 rating by FL1 does not imply that the lamp is suitable for use as a diver's light since the test protocol examines function of the light only after immersion, not during immersion. -
White Light Emission from Fluorescent Sic with Porous Surface
www.nature.com/scientificreports OPEN White Light Emission from Fluorescent SiC with Porous Surface Weifang Lu1, Yiyu Ou 1, Elisabetta Maria Fiordaliso2, Yoshimi Iwasa3, Valdas Jokubavicius4, Mikael Syväjärvi4, Satoshi Kamiyama3, Paul Michael Petersen1 & Haiyan Ou1 Received: 24 May 2017 We report for the frst time a NUV light to white light conversion in a N-B co-doped 6H-SiC (fuorescent Accepted: 14 August 2017 SiC) layer containing a hybrid structure. The surface of fuorescent SiC sample contains porous Published: xx xx xxxx structures fabricated by anodic oxidation method. After passivation by 20 nm thick Al2O3, the photoluminescence intensity from the porous layer was signifcant enhanced by a factor of more than 12. Using a porous layer of moderate thickness (~10 µm), high-quality white light emission was realized by combining the independent emissions of blue-green emission from the porous layer and yellow emission from the bulk fuorescent SiC layer. A high color rendering index of 81.1 has been achieved. Photoluminescence spectra in porous layers fabricated in both commercial n-type and lab grown N-B co-doped 6H-SiC show two emission peaks centered approximately at 460 nm and 530 nm. Such blue- green emission phenomenon can be attributed to neutral oxygen vacancies and interface C-related surface defects generated dring anodic oxidation process. Porous fuorescent SiC can ofer a great fexibility in color rendering by changing the thickness of porous layer and bulk fuorescent layer. Such a novel approach opens a new perspective for the development of high performance and rare-earth element free white light emitting materials. -
Infrared Lighting Does Not Suppress Catch of Codling Moth (Lepidoptera: Tortricidae) in Pheromone-Baited Monitoring Traps
The Great Lakes Entomologist Volume 48 Numbers 3/4 -- Fall/Winter 2015 Numbers 3/4 -- Article 10 Fall/Winter 2015 October 2015 Infrared Lighting Does Not Suppress Catch of Codling Moth (Lepidoptera: Tortricidae) in Pheromone-Baited Monitoring Traps C. G. Adams Michigan State University, [email protected] P. S. McGhee Michigan State University J. R. Miller Michigan State University Follow this and additional works at: https://scholar.valpo.edu/tgle Part of the Entomology Commons Recommended Citation Adams, C. G.; McGhee, P. S.; and Miller, J. R. 2015. "Infrared Lighting Does Not Suppress Catch of Codling Moth (Lepidoptera: Tortricidae) in Pheromone-Baited Monitoring Traps," The Great Lakes Entomologist, vol 48 (3) Available at: https://scholar.valpo.edu/tgle/vol48/iss3/10 This Peer-Review Article is brought to you for free and open access by the Department of Biology at ValpoScholar. It has been accepted for inclusion in The Great Lakes Entomologist by an authorized administrator of ValpoScholar. For more information, please contact a ValpoScholar staff member at [email protected]. Adams et al.: Infrared Lighting Does Not Suppress Catch of Codling Moth (Lepid 168 THE GREAT LAKES ENTOMOLOGIST Vol. 48, Nos. 3 - 4 Infrared Lighting Does Not Suppress Catch of Codling Moth (Lepidoptera: Tortricidae) in Pheromone-Baited Monitoring Traps C. G. Adams1,*, P. S. McGhee1, and J. R. Miller1 Abstract Video cameras are increasingly being used to record insect behaviors in the field over prolonged intervals. A nagging question about crepuscular and nocturnal recordings is whether or not infrared light emitted by such cameras to illuminate the scene influences the behaviors of the subjects or study outcomes. -
Basic Physics of the Incandescent Lamp (Lightbulb) Dan Macisaac, Gary Kanner,Andgraydon Anderson
Basic Physics of the Incandescent Lamp (Lightbulb) Dan MacIsaac, Gary Kanner,andGraydon Anderson ntil a little over a century ago, artifi- transferred to electronic excitations within the Ucial lighting was based on the emis- solid. The excited states are relieved by pho- sion of radiation brought about by burning tonic emission. When enough of the radiation fossil fuels—vegetable and animal oils, emitted is in the visible spectrum so that we waxes, and fats, with a wick to control the rate can see an object by its own visible light, we of burning. Light from coal gas and natural say it is incandescing. In a solid, there is a gas was a major development, along with the near-continuum of electron energy levels, realization that the higher the temperature of resulting in a continuous non-discrete spec- the material being burned, the whiter the color trum of radiation. and the greater the light output. But the inven- To emit visible light, a solid must be heat- tion of the incandescent electric lamp in the ed red hot to over 850 K. Compare this with Dan MacIsaac is an 1870s was quite unlike anything that had hap- the 6600 K average temperature of the Sun’s Assistant Professor of pened before. Modern lighting comes almost photosphere, which defines the color mixture Physics and Astronomy at entirely from electric light sources. In the of sunlight and the visible spectrum for our Northern Arizona University. United States, about a quarter of electrical eyes. It is currently impossible to match the He received B.Sc. -
Federal Register/Vol. 82, No. 12/Thursday, January 19, 2017
7322 Federal Register / Vol. 82, No. 12 / Thursday, January 19, 2017 / Rules and Regulations (diameter less than or equal to 1 inch) Marine lamp means a lamp that is marketed as a sign service lamp, and has as defined in ANSI C79.1–2002 designed and marketed for use on boats a maximum rated wattage of 15 watts. (incorporated by reference; see § 430.3), and can operate at or between 12 volts Silver bowl lamp means a lamp that nominal overall length less than 12 and 13.5 volts. has an opaque reflective coating applied inches, and that are not compact Marine signal service lamp means a directly to part of the bulb surface that fluorescent lamps (as defined in this lamp that is designed and marketed for reflects light toward the lamp base and section); marine signal service applications. that is designed and marketed as a silver (26) Traffic signal lamps; * * * * * bowl lamp. (27) Incandescent reflector lamps. Mine service lamp means a lamp that * * * * * General service light-emitting diode is designed and marketed for mine Specialty MR lamp means a lamp that (LED) lamp means an integrated or non- service applications. has an MR shape as defined in ANSI integrated LED lamp designed for use in * * * * * C79.1–2002 (incorporated by reference; general lighting applications (as defined Non-integrated lamp means a lamp see § 430.3), a diameter of less than or in this section) and that uses light- that is not an integrated lamp. equal to 2.25 inches, a lifetime of less emitting diodes as the primary source of * * * * * than or equal to 300 hours, and that is light. -
Single Ended Lamp
SINGLE-ENDEDSINGLE-ENDED LAMPLAMP The Short Arc Gap USHIO’s Sōlarc® single-ended lamps allow the equipment designer to capitalize on the lamp’s unique short arc length. At 1.27mm, with a peak luminance at the cathode, the lamp begins to approximate a point source. Coupled with carefully designed lenses or reflectors with maximum light capture and the appropriate focus, the lamp can deliver high-intensity light to tightly controlled or divergent beam applications. The figure below shows the luminous intensity distribution of the arc. The two sources of peak intensity lie near the electrode tips. Highest Efficacy Metal halide lamps are inherently very efficient, providing two to three times the efficacy of either halogen or xenon lamps. Optimizing the optical system using the short arc can provide an efficiency increase in many applications, allow- ing the Sōlarc lamp to deliver as much light as a halogen lamp with four to five times more wattage. High efficacy plus the resultant decreased demand for power allow the equipment designer to develop miniature, lighter weight, portable and even battery-powered product configurations. White Light Sōlarc lamps produce a color temperature in the range of 5,000K–7,000K, putting it in the same range as the sun. For comparison, halogen lamps normally operate in the 3,000K–3,200K range and incandescent lamps in the 2,800K- 2,900K range. In visible terms, the lower color temperature dictates more red or yellow in the light. The higher color temperature enables realistic visualization of color. While it is possible to operate halogen lamps up to 4,300K by the use of filters, it is only at a severe reduction in lamp life and output. -
Products Catalog Index
Products Catalog Index PART NO. MANUFACTURER DESCRIPTION URL PRICE LN166 N/A The Optical Devices Data http://www.searchdatasheet.com/LN166-datasheet.html QUOTE Book (Japanese) LN166 Panasonic Light Emitting Diodes http://www.searchdatasheet.com/LN166-datasheet.html QUOTE LN166 Panasonic Light Emitting Diodes http://www.searchdatasheet.com/LN166-datasheet.html QUOTE LED LN166 Panasonic Light Emitting Diodes http://www.searchdatasheet.com/LN166-datasheet.html QUOTE LED LN166 Panasonic GaAs Infrared Light http://www.searchdatasheet.com/LN166-datasheet.html QUOTE Emitting Diode LN170WP38 N/A The Optical Devices Data http://www.searchdatasheet.com/LN170WP38-datasheet.html QUOTE Book (Japanese) LN170WP38 Panasonic Dome-Style LED Lamp http://www.searchdatasheet.com/LN170WP38-datasheet.html QUOTE LN172 N/A The Optical Devices Data http://www.searchdatasheet.com/LN172-datasheet.html QUOTE Book (Japanese) LN172 Panasonic Light Emitting Diodes http://www.searchdatasheet.com/LN172-datasheet.html QUOTE LN172 Panasonic GaAlAs Infrared Light http://www.searchdatasheet.com/LN172-datasheet.html QUOTE Emitting Diode LN172 Panasonic Light Emitting Diodes http://www.searchdatasheet.com/LN172-datasheet.html QUOTE LED LN172 Panasonic GaAlAs Infrared Light http://www.searchdatasheet.com/LN172-datasheet.html QUOTE Emitting Diode LN173WP38 N/A The Optical Devices Data http://www.searchdatasheet.com/LN173WP38-datasheet.html QUOTE Book (Japanese) LN173WP38 Panasonic Rectangular LED Lamp http://www.searchdatasheet.com/LN173WP38-datasheet.html QUOTE LN175 N/A The