2010/2011 Forschungsbericht Scientific Report

Leibniz-inStitut FüR PFLanzengenetik und kuLtuRPFLanzenFoRSchung (iPk) 2010/2011 Scientific Report Forschungsbericht/

Leibniz-inStitut FüR PFLanzengenetik und kuLtuRPFLanzenFoRSchung (iPk) Forschungsbericht Stiftung des öffentlichen Rechts Scientific Report 2010/2011 corrensstraße 3 d-06466 Stadt Seeland, ot gatersleben Leibniz Institute of Plant Genetics and Governing Board Chair: Crop Plant Research (IPK) MinDirig Dr. Joachim Welz Vice Chair: Corrensstraße 3 RD Peter Hassenbach D-06466 Gatersleben Scientific Advisory Board Germany Chair: Phone: +49 (0)39482 50 Board of Directors Prof. Christian Jung Fax: +49 (0)39482 5500 Prof. Andreas Graner 1) Email: [email protected] Managing Director Internet: http://www.ipk-gatersleben.de Genebank Advisory Board Sybille-Andrea Lorenz 1) Chair: As of 1 January 2010 Administrative Director PD Dr. Christiane Gebhardt Managing Office Prof. Thomas Altmann Roland Schnee Prof. Ingo Schubert Personnel Council Prof. Nicolaus von Wirén Chair: Thomas Kruse

Dept. of Genebank Dept. of Cytogenetics and Genome Analysis Dept. of Molecular Dept. of Physiology and Dept. of Administration Genetics Cell Biology and Central Services Head: Prof. Ingo Schubert Head: Prof. Andreas Graner Head: Prof. Thomas Altmann Head: Prof. Nicolaus von Wirén Head: Sybille-Andrea Lorenz

Programmes

Characterisation and Management and Taxonomy and Cytogenetics Genome Analysis Documentation Evaluation Evolution Prof. Ingo Schubert Dr. Patrick Schweizer Prof. Andreas Graner Dr. Andreas Börner Dr. Frank Blattner

Groups

Genome Diversity Resources Genetics and Experimental Karyotype Evolution Transcriptome Analysis Heterosis Molecular Plant Nutrition Personnel Prof. Andreas Graner Reproduction Taxonomy Prof. Ingo Schubert Dr. Patrick Schweizer Prof. Thomas Altmann Prof. Nicolaus von Wirén Juliane Becker Dr. Nils Stein Dr. Andreas Börner Dr. Frank Blattner Chromosome Structure Gene and Genome Mapping Seed Development Applied Biochemistry Finances Dr. Winfriede Weschke Genebank Documentation In vitro Storage and Taxonomy of Plant and Function Dr. Marion Röder Dr. Hans-Peter Mock Martina Liewald Dr. Helmut Knüpffer Genetic Resources Cryopreservation Dr. Andreas Houben Gene Regulation Dr. Joachim Keller Dr. Frank Blattner (prov.) Bioinformatics and Structural Cell Biology Technology Transfer and Apomixis Dr. Helmut Bäumlein Plant Architecture Information Technology Dr. Michael Melzer Legal Matters (BMBF-funded Research Group) Dr. Timothy F. Sharbel Dr. Tankred Schuhmann Satellite Collections North Dr. Uwe Scholz Phytoantibodies Dr. Thorsten Schnurbusch Dr. Klaus Dehmer Dr. Udo Conrad Plant Reproductive Biology Materials, Management and Genome Plasticity Dr. Jochen Kumlehn Dr. Renate Schmidt Expression Mapping General Services Dr. Lothar Altschmied Ursula Deppner Yeast Genetics Epigenetics Plant Bioinformatics Prof. Gotthard Kunze Dr. Michael F Mette Buildings and Equipment Prof. Falk Schreiber Herwig Bierstedt Systems Biology Data Inspection In vitro Differentiation (BMBF-funded Research Group) (Junior Research Group) Experimental Fields and Prof. Anna M Wobus Dr. Björn H. Junker Dr. Marc Strickert Nurseries Peter Schreiber Hybrid Wheat (BMBF-funded Research Group) Dr. Mario Gils

Plant Genome Resources Centre (PGRC) Coordinator: Dr. Patrick Schweizer

Bioinformatics Coordinator: Prof. Falk Schreiber 1) Management

Leibniz Institute of Plant Genetics and Governing Board Chair: Crop Plant Research (IPK) MinDirig Dr. Joachim Welz Vice Chair: LCorreenibnisstraße z3 Institute of Plant Genetics and GoDrve. Henrnki ngvan LBieomaptr d Scientific Advisory Board D-06466 Gatersleben Chair: Chair: GeCrrmopany Plant Research (IPK) MinDirig Dr. Joachim Welz Prof. Christian Jung Phone: +49 (0)39482 50 BoardV iocef CDhairirec: tors Fax: +49 (0)39482 5500 Corrensstraße 3 PDrorf.. HAenndkrea vans Gr Lianeemprt 1 ) Scientific Advisory Board Email: [email protected] D-06466 Gatersleben Managing Director Genebank A Cdhaviris: ory Board Internet: http://www.ipk-gatersleben.de Chair: Germany Sybille-Andrea Lorenz 1) Prof. Christian Jung Phone: +49 (0)39482 50 PD Dr. Christiane Gebhardt As of 3 1 December 201 1 Bo Aadmrdin isotrfa tDiveir Decirecttoor s Fax: +49 (0)39482 5500 Prof. Andreas Graner 1) Email: [email protected] Prof. Thomas Altmann Managing Office Managing Director Genebank Advisory Board Internet: http://www.ipk-gatersleben.de Roland Schnee Prof. Ingo Schubert Personn Cehali rC: ouncil Sybille-Andrea Lorenz 1) Prof. Nicolaus von Wirén PD CDhar. iCr: hTrhoistimaneas GKrebhause rdt As of 3 1 December 201 1 Administrative Director Managing Office Prof. Thomas Altmann Roland Schnee Prof. Ingo Schubert Personnel Council Dept. of Genebank Dept. of CytogeneticsPro f.a Nndico lauGes vnoon mWireé nA nalysis Dept. of Molecula rC hair: Tho Dmaesp Ktr.u soef Physiology and Dept. of Administration Genetics Cell Biology and Central Services Head: Prof. Ingo Schubert Head: Prof. Andreas Graner Head: Prof. Thomas Altmann Head: Prof. Nicolaus von Wirén Head: Sybille-Andrea Lorenz Dept. of Genebank Dept. of Cytogenetics and Genome Analysis Dept. of Molecular Dept. of Physiology and Dept. of Administration Programmes Genetics Cell Biology and Central Services Head: Prof. Ingo Schubert Characterisation and HeadManag: Proef.m Aendntr eaansd G raner Taxonomy and Cytogenetics Genome Analysis Head: Prof. Thomas Altmann Head: Prof. Nicolaus von Wirén Head: Sybille-Andrea Lorenz Documentation Evaluation Evolution Prof. Ingo Schubert Dr. Patrick Schweizer Prof. Andreas Graner Dr. Andreas Börner Dr. FrPanrkog Blraattmmner es

Characterisation and Management and Taxonomy and Cytogenetics GenomGeroup Anasl ysis Documentation Evaluation Evolution Prof. Ingo Schubert Dr. Patrick Schweizer PGroenof. Andmerea Dsi veGrranesitry DRresou. Andrearcses B öGrneenre tics and DErxpe. Franrimk Belnatttanel r Karyotype Evolution Transcriptome Analysis Heterosis Molecular Plant Nutrition Personnel Prof. Andreas Graner Reproduction Taxonomy Prof. Ingo Schubert Dr. Patrick Schweizer Prof. Thomas Altmann Prof. Nicolaus von Wirén Wilma Klaasen-van Husen Dr. Nils Stein Dr. Andreas Börner Dr. Frank Blattner Groups Chromosome Structure Gene and Genome Mapping Seed Development Applied Biochemistry Finances

Genoenebmaen Dk iDveocursitmyen tation RInesou vitror cSesto rGageene anticds and ETaxoxpenriommeyn toafl Plant Kandaryo Functypeti oEnvo lution DTr.ans Marcionrip Rtoödemre Analysis HDre. tWerinofrsiedeis Weschke MDro. Hlecuans-lPaer tPerl anMotc Nk utrition PMeartsoinan Lnieewl ald Dr. Helmut Knüpffer Cryopreservation Genetic Resources Prof. Thomas Altmann Prof. Andreas Graner Reproduction Taxonomy PDro. f.A ndIngorea Ss cHhuoubbeertn Dr. Patrick Schweizer Gene Regulation Prof. Nicolaus von Wirén TechWilman Kolaalosgyen -Tvanran Hsufseern and Dr. Nils Stein Dr. AJoandcreahims BKöerllneer r Dr. Frank Blattner (prov.) Bioinformatics and Structural Cell Biology P lant Architecture Apomixis Dr. Helmut Bäumlein Legal Matters Chromosome Structure GIneneform andation G enoTechnmeo Mlogappy ing Seed Development ADrpp. Mliicedhae Bl Miocheelzer mistry Finances (BMBF-funded Research Group) Dr. Timothy F. Sharbel Dr. Tankred Schuhmann Genebank Docu mentation ISna vteitllirote S Ctoorllageect ianonsd North Taxonomy of Plant Dr. MUwareion Sc Rhoödelz r PDrhy. Wtoinafrniedetibo Wdeiscesh ke Dr. Hans-Peter Mock Martina Liewald Dr. Thorsten Schnurbusch and Function Dr. Helmut Knüpffer CDrr.yop Klaurse Dseehrvametri on Genetic Resources Dr. Udo Conrad Plant Reproductive Biology GDreno. Andmreae sP lHasoutbeicnit y Gene Regulation TechMaterniaollso,gy M anageTransfmeren andt and Dr. Joachim Keller Dr. Frank Blattner (prov.) Bioinformatics and SDrt.r Jucocthenura lK Cumellleh Bni ology P lant Architecture ADrpo. Rmenaixteis S chmidt PDrl.an Het lmBuiot iBnäufomrmleiant ics LegaGenelr Mala Stteerrvsice s Information Technology Dr. Michael Melzer (BMBF-funded Research Group) Dr. Timothy F. Sharbel Prof. Falk Schreiber DUrrs. uTlana Dkrepedpn Secrhuh mann Satellite Collections North Dr. Uwe Scholz Phytoantibodies Yeast Genetics Dr. Thorsten Schnurbusch Epigenetics Dr. Klaus Dehmer Dra. tUado Inspec Conradti on Prof. Gotthard Kunze Dr. Michael F Mette Plant Reproductive Biology MBuailtedriingsals, andManage Equmipenment andt Genome Plasticity (Junior Research Group) Dr. Jochen Kumlehn HGeenerwigr aBlie Srsetedrvti ces Dr. Renate Schmidt DPrl.an Swt eBtliaonian fFroiredemal tics Systems Biology Prof. Falk Schreiber Ursula Deppner Y(BeasMBFt -Gfundeneedt iRcess earch Group) Epigenetics Hybrid Wheat Experimental Fields and Data Inspection DPro. f.B jGoörntt Hha. rJdun Kkunerz e Dr. Michael F Mette (BMBF-funded Research Group) BNuilrsedingsries and Equipment (Junior Research Group) Dr. Mario Gils HPetrewri gS cBhierersibetedr t Dr. Swetlana Friedel Systems Biology Image Analysis (BMBF-funded Research Group) Hybrid Wheat Experimental Fields and (Junior Research Group) Dr. Björn H. Junker (BMBF-funded Research Group) Nurseries Dr. Christian Klukas Dr. Mario Gils Peter Schreiber Stress Genomics (ImIZNage-fund Aenad Jlysuniiosr Research Gr(Junoupior) Research Group) Dr. NCehsries tiSanreen Kluivkaassu lu Stress Genomics (IZN-funded Junior Research Group) Dr. Nese Sreenivasulu

Plant Genome Resources Centre (PGRC) Coordinator: Dr. Patrick Schweizer

Bioinformatics Coordinator: Prof. Falk Schreiber 1) Management

Plant Genome Resources Centre (PGRC) Coordinator: Dr. Patrick Schweizer

Bioinformatics Coordinator: Prof. Falk Schreiber 1) Management

Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)

Forschungsbericht Scientific Report 2010/2011

Gatersleben, Dezember 2011 Impressum

Herausgeber: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) Corrensstraße 3 D-06466 Stadt Seeland, OT Gatersleben Tel.: +49 (0)39482-50 Fax: +49 (0)39482-5500 Internet: http://www.ipk-gatersleben.de Geschäftsführender Direktor: Prof. Dr. Andreas Graner Administrative Leiterin: Sybille-Andrea Lorenz Redaktion: Roland Schnee Gesamtherstellung: Koch-Druck, Halberstadt Bilder: Vorderseite Umschlag: Sam Rey (2), IPK Gatersleben (1) Rückseite Umschlag: Flugdienst Magdeburg Inhaltsverzeichnis/Contents

Inhaltsverzeichnis/Contents Seite/Page Inhaltsverzeichnis/Contents Seite/Page

Organigramm 2010/ Vorträge und Poster/Lectures and Posters ...... 165 Organisation of the Institute 2010 ...... u2 Eingeladene Vorträge auf internationalen Tagungen (Auswahl)/ Invited Lectures at Vorwort/Introduction ...... 4 International Conferences (Selection) ...... 165 Vorträge/Lectures ...... 168 Das Leibniz-Institut für Pflanzengenetik Poster/Posters ...... 186 und Kulturpflanzenforschung (IPK)/ The Leibniz Institute of Plant Genetics Vom Institut organisierte Tagungen and Crop Plant Research (IPK) ...... 6 und Veranstaltungen/ Conferences and Meetings Organised by IPK ...... 208 Das Institut in den Jahren 2010 und 2011/ The Institute in 2010 and 2011 ...... 10 Beteiligung an der Organisation externer Veranstaltungen/ Verwaltung und Infrastruktur/ Participation in Organising Administration and Infrastructure ...... 18 External Meetings ...... 210 Personal/Staff ...... 18 Budget ...... 18 Ehrungen, Preise/Honours, Awards ...... 212 Drittmitteleinnahmen/Third Party Funding ...... 18 Technologietransfer/Technology Transfer ...... 20 Arbeitsaufenthalte von Gästen im IPK/ Raumprogramm und Baumaßnahmen/ Guest Researchers at IPK ...... 213 Facilities and Constructions ...... 20 Wissenschaftliche Bibliothek/ Arbeitsaufenthalte von IPK-Wissen- Scientific Library ...... 20 schaftlern in anderen Einrichtungen/ Stays of IPK Researchers at Other Institutions ...... 222 Forschungsberichte der Abteilungen/ Research Reports of the Departments Lehrtätigkeit/Teaching ...... 224 Abteilung Genbank/ Mitarbeit an wissenschaftlichen Department of Genebank ...... 22 Zeitschriften/Editing Scientific Journals ...... 228 Abteilung Cytogenetik und Genomanalyse/ Department of Cytogenetics Tätigkeit in Gremien/Activities in Boards ...... 229 and Genome Analysis ...... 54 Abteilung Molekulare Genetik/ Öffentlichkeitsarbeit/Public Relations ...... 231 Department of Molecular Genetics ...... 88 Informationsveranstaltungen und Führungen/ Abteilung Physiology und Zellbiologie/ Informative Events and Guided Tours ...... 231 Department of Physiology and Cell Biology ...... 124 Pressemitteilungen/Press Releases ...... 242 Pflanzengenom-Ressourcen-Centrum (PGRC)/ Presse- und Medienarbeit/ Plant Genome Resources Centre (PGRC) ...... 151 Contributions in Press and Media ...... 243 Die Entwicklung der Bioinformatik am IPK/ Messen und Ausstellungen/ The Development of Bioinformatics at IPK ...... 153 Fairs and Exhibitions ...... 247 Das Doktorandenprogramm/ The PhD Programme ...... 155 Übersicht Drittmittelprojekte/ Overview of Additional Funding ...... 249 Kolloquien und Seminare/ Colloquia and Seminars ...... 157 Gremien und Mitarbeiter/-innen Gatersleben Lectures ...... 157 in speziellen Funktionen/ Vavilov- und PGRC-Seminare/ Boards of IPK and Employees Vavilov and PGRC Seminars ...... 158 with Special Responsibilities ...... 283 Vavilov-Vortragsabende/Vavilov Lectures ...... 159 Genetische Seminare/Genetics Seminars ...... 160 Organigramm 2011/ Zellbiologische Seminare/ Organisation of the Institute 2011 ...... u3 Cell Biology Seminars ...... 162 Waterman-Seminare/Waterman Seminars ...... 163 Vorwort

Vorwort Introduction

Im vor uns liegenden Jahr jährte sich die Neugründung des In- In 2012 IPK will celebrate the twentieth anniversary of its re- stituts am 1. Januar 1992 zum 20. Mal. Dieses Jubiläum gibt An- foundation, a milestone which prompts us to take a retrospec- lass zur Rückschau auf eine Zeit, in der das Institut sich, zweifels- tive look back over the period during which the Institute has ohne mit großem Erfolg, in der neu gestalteten gesamtdeut- succeeded so well in cementing a firm place in the much al- schen Wissenschaftslandschaft etablieren konnte. Basierend auf tered German scientific landscape.B uilding on the enthusiasm, dem Einsatz aller Mitarbeiter und der Unterstützung durch Land knowledge and skills of our employees, and based on continu- und Bund nimmt das IPK heute eine weithin sichtbare Rolle in ing financial support from both the State of Saxony-Anhalt der internationalen Pflanzenforschung ein. Die Forschungsar- and the Federal Government, IPK has achieved a high degree beiten reichen von der Bearbeitung grundlegender Fragestel- of visibility in the international plant science community. Its lungen der Samenentwicklung und der Fortpflanzungsbiologie, activities span the range of plant science research, from fun- der Erforschung spezieller Chromosomenstrukturen oder dem damental topics such as seed development and reproductive Studium von Artbildungs- und Anpassungsmechanismen bis biology, chromosome structure and function, adaptation and hin zu angewandten Fragestellungen mit Bezügen zur Pflanzen- speciation, to more applied ones such as crop improvement, züchtung und Pflanzenernährung sowie zur Biotechnologie. plant nutrition and biotechnology.

Seit dem Jahr der Neugründung des IPK stieg die Erdbevölke- Since 1992, the size of the world’s population has risen by 1.5 rung um weitere 1,5 Milliarden auf aktuell 7 Milliarden Men- billion to reach its current level of seven billion, equivalent to a schen an. Die tägliche Bevölkerungszunahme beträgt damit daily increase of some 200,000 people, all of whose livelihoods rund 200.000 Menschen, deren Lebensgrundlagen es zu si- need to be secured. Given that the area of arable land is neces- chern gilt. Angesichts der weltweit begrenzten Verfügbarkeit sarily capped, the growing demand for food, renewable energy landwirtschaftlicher Nutzflächen erfordert die Deckung des and renewable materials requires a steep change in crop pro- Bedarfs an Nahrungsmitteln, erneuerbaren Energien und ductivity. The IPK research programme makes a unique contri- nachwachsenden Rohstoffen eine leistungsfähige Pflanzen- bution in the area of genetic-based crop improvement, with produktion. Die am Institut erarbeiteten Erkenntnisse liefern a special emphasis on increasing yield, nutrient use efficiency Beiträge für die züchterische Verbesserung von Kulturpflan- and the level of resistance and/or tolerance to both biotic and zen, insbesondere im Hinblick auf die Steigerung des Ertrags, abiotic stress. The latter is particularly important in the context die Verbesserung der Nährstoffeffizienz und die Erhöhung der of global climate change, a process which was hard to ignore Resistenz bzw. Toleranz gegenüber biotischen und abiotischen during 2010, globally the warmest year on record. Stressfaktoren. Letzteren kommt angesichts der Veränderung des Klimas ‑ 2010 war das wärmste Jahr seit Beginn der Wetter- Research targeted at elucidating the structure and under- aufzeichnungen ‑ eine besonders große Bedeutung zu. standing the functioning of key crop genomes is likely to drive the utilization of the genetic resources maintained in the IPK Die am Institut durchgeführten Forschungsarbeiten zur Aufklä- Genebank. Gene technology can be expected to play an im- rung der Struktur und Funktion der Genome wichtiger Kultur- portant role, both by allowing the means to study a number pflanzen werden langfristig die Grundlage für dieN utzung der in of the most fundamental aspects of plant biology, and by der Genbank erhaltenen pflanzengenetischen Ressourcen dar- helping to improve the expression of crop traits (such as, for stellen. Hierbei gilt es auch die Potenziale der Grünen Gentech- example, abiotic stress tolerance) which have proven so dif- nik auszuschöpfen. Dies betrifft zum einen den Einsatz gentech- ficult to manipulate using conventional breeding technology. nischer Verfahren als Hilfsmittel bei der Bearbeitung verschie- A further potential area for the application of transgenesis in denster wissenschaftlicher Fragestellungen und zum anderen plants lies in developing bioreactors able to synthesize novel die Nutzung und Weiterentwicklung der Grünen Gentechnik zur chemical compounds, antigens or antibodies in an inexpensive Verbesserung pflanzlicher Merkmale undE igenschaften, der Ent- and environmentally benign manner. The controversial (at least wicklung verbesserter Züchtungsverfahren sowie die Verwen- in Europe) nature of genetically modified (GM) plants and the dung von Pflanzen als Bioreaktor für die Erzeugung neuartiger dwindling political support for this technology notwithstand-

4 Stoffe, wie z. B. Antikörpern. Trotz der kontroversen Diskussion ing, the Institute recognizes its major scientific responsibility zur Grünen Gentechnik in Europa und ungeachtet der schwin- to continue leading the public-funded research effort aimed at denden politischen Unterstützung der Grünen Gentechnik sieht assessing the true potential of GM technology. sich das Institut weiterhin in der wissenschaftlichen Verantwor- tung, die Potenziale dieser Technologie zu erforschen. The year 2012 also marks the twentieth anniversary of the signing of the Convention on Biodiversity (CBD) by 193 parties, a Ebenfalls im Jahr 2012 jährt sich die Verabschiedung der Kon- treaty which attempted to ensure the long-term conservation, vention über die Biologische Vielfalt zum 20. Mal. Mit dem Ab- utilization and valorization of biodiversity, as well as providing kommen sollen der Erhalt der biologischen und genetischen a framework to regulate access to germplasm and introduce Vielfalt, ihre Nutzung und Nutzbarmachung sowie der Zugang mechanisms for benefit sharing. The IPK Genebank, which cu- und der Vorteilsausgleich langfristig gesichert werden. Die rates a range of agricultural and horticultural crop species, is Bundeszentrale Ex-situ-Genbank am IPK stellt einen zentralen a central component of the German “National Programme for Pfeiler zum Erreichen der genannten Ziele im Zuge des „Natio- the Conservation and Utilization of Plant Genetic Resources”. nalen Fachprogramms zur Erhaltung und nachhaltigen Nut- The continuing high demand of the international plant science zung pflanzengenetischer Ressourcen landwirtschaftlicher und community for samples of Genebank accessions is a measure gartenbaulicher Kulturpflanzen“ dar. Die großeN achfrage nach of the importance of the collection for research, crop breeding Material aus der Genbank stellt einen Beleg für die Bedeutsam- and other uses. keit der IPK-Sammlungen für Forschung, Züchtung sowie weitere Anwendungen dar. There can be no doubt that securing human livelihood requires the continuing improvement of our crop plants. High profile Ohne Zweifel ist die Sicherung der Lebensgrundlagen der fundamental research, which is predicated first and foremost Menschen an die weitere Verbesserung der Leistungsfähigkeit on scientific excellence, lays the groundwork for developing so- unserer Kulturpflanzen gebunden. Leistungsfähige, ausschließ- lutions to many of the most pressing societal challenges. At the lich an wissenschaftlicher Exzellenz orientierte Grundlagenfor- same time, there is a need to convert the knowledge emerging schung ist der Nährboden für die Erarbeitung von Lösungen, from basic research into practical applications. This paradigm die in den kommenden Jahrzehnten verfügbar sein müssen. requires that the genetic, biochemical and cell biological proc- Auf diesem Weg gilt es auch, den Erkenntnisgewinn aus der esses which underlie agronomic performance and environ- Grundlagenforschung so rasch wie möglich in Anwendungen mental adaptation be elucidated as a priority. The enormous zu überführen. Dies erfordert die Aufklärung der genetischen, technical advances achieved in the ability to both sequence biochemischen und zellbiologischen Prozesse, die an der Aus- DNA and to identify primary and secondary metabolites cur- prägung der Leistungsmerkmale von Pflanzen beteiligt sind rently greatly outstrips our capacity to analyse variation in phe- und ihre Anpassung an unterschiedliche Umweltbedingungen notype and specific plant traits. This gap is being addressed bewirken. Dem enormen technischen Fortschritt auf dem Ge- by the Institute through a series of substantial investments in biet der DNA-Sequenzierung und der Analyse von primären the technical infrastructure required to systematize the assess- und sekundären Metaboliten des pflanzlichen Stoffwechsels ment of phenotype. The establishment of highly automated standen bisher nur begrenzte Möglichkeiten zur Erfassung image analysis systems should allow, for example, an accurate phänotypischer Merkmale gegenüber. Aus diesem Grund hat description of temporal growth patterns. The hoped-for im- das Institut in den vergangenen Jahren neben dem kontinu- provements in phenotypic analysis will have a major beneficial ierlichen Ausbau der experimentellen Laborinfrastruktur um- impact on the measurement of those quantitative traits which fangreiche Investitionen im Gewächshausbereich vorgenom- lie at the heart of crop productivity. men. Mit Hilfe modernster Methoden der Bilderfassung wird es möglich sein, weitestgehend unabhängig von störenden Against this backdrop, the IPK research programme over the Umwelteinflüssen, zeitliche Wachstumsverläufe systematisch past two years has yielded a series of exciting and important re- zu erfassen. Die angestrebten Verbesserungen in der phänoty- sults, addressing both fundamental and applied research ques- pischen Analyse werden vor allem die Aufklärung quantitativ tions. Further details are given in the research report which fol- vererbter Merkmalskomplexe ermöglichen. lows this brief overview.

Vor diesem Hintergrund wurden in den vergangenen beiden Jahren wieder eine Reihe interessanter und wichtiger For- Andreas Graner schungsergebnisse erzielt, welche wichtige Beiträge sowohl Managing Director zur Aufklärung grundlegender als auch angewandter Frage- stellungen lieferten. Einzelheiten hierzu finden Sie in dem vor- liegenden Forschungsbericht.

Andreas Graner Geschäftsführender Direktor

5 Das Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)/ The Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

Das Leibniz-Institut für The Leibniz Institute of Pflanzengenetik und Kultur- Plant Genetics and pflanzenforschung (IPK) Crop Plant Research (IPK)

Aufgabenstellung und Finanzierung Objectives and Funding Das IPK wurde auf der Grundlage von Vorgängereinrichtungen The IPK was formally re-established in 1992 as a Foundation 1992 als eine Stiftung des öffentlichen Rechts gegründet und under Public Law, continuing an unbroken tradition reaching ist Mitglied der Leibniz-Gemeinschaft. Sein Zuwendungsbedarf back to the Kaiser-Wilhelm-Institute of Crop Plant Research, wird gemäß Artikel 91 b des Grundgesetzes nach dem Finan- which was founded in 1943 near Vienna (Austria) and moved zierungsmodell der „Blauen Liste“ zu gleichen Teilen von Bund to Gatersleben in 1945. It is administered under the legal and und Sitzland (plus Länderanteile) erbracht. Zuwendungsgeber administrative supervision of the State of Saxony-Anhalt. Ac- ist das Land Sachsen-Anhalt, vertreten durch die Ministerin für cording to article 91b of the Federal Constitution its funding Wissenschaft und Wirtschaft. is provided by Ministry of Science and Economy of the State of Saxony-Anhalt (along with contributions from other German „Zweck der Stiftung ist die Förderung von Wissenschaft und For- States), and by the Federal Ministry of Education and Research. schung. Ihre Aufgabe ist, grundlagen- und anwendungsorientierte Forschung auf den Gebieten der Pflanzengenetik und Kulturpflan- The Institute‘s statutes state: ‘The mission of the Foundation is zenforschung zu betreiben. Ihre wissenschaftlichen Schwerpunkte the advancement of science and research. Its goals are to carry liegen insbesondere auf der Erarbeitung neuer Erkenntnisse über out basic and application-oriented research in the fields of plant Struktur, Funktion und Evolution des Erbmaterials, auf der Erhal- genetics and crop plant research. Special emphasis is given to the tung, Erforschung und Erschließung der erblichen Vielfalt von Kul- generation of new knowledge on the structure, function and evo- turpflanzen, ihrer Vorfahren und Verwandten sowie auf Beiträgen lution of genetic material, on the preservation, research and use zur Züchtungsgenetik im Vorfeld der praktischen Pflanzenzüch- of the biodiversity of crop plants and their wild relatives, as well as tung. Ein wesentliches Anliegen der Stiftung ist die interdisziplinä- contributions to applied genetics with regard to crop breeding. A re Zusammenarbeit der verschiedenen in ihr vertretenen biologi- major concern of the Foundation is to encourage interdisciplinary schen Fachrichtungen.“ (zitiert aus der IPK-Satzung) cooperation of the various biological disciplines in the Institute.’ (translated from the German original) Durch die Kombination von Grundlagen- und angewandter Forschung mit Fokus auf Kulturpflanzen sieht sich das Insti- By the combination of basic and applied research with empha- tut gleichermaßen prädestiniert und verpflichtet, Beiträge zur sis on cultivated crop species the Institute considers itself both Lösung zukünftiger Herausforderungen auf den Gebieten der predestinated and obliged to develop contributions to solve Nahrungssicherung, der Erzeugung nachwachsender Rohstof- future challenges regarding food security, the generation of fe und erneuerbarer Energien, der Nachhaltigkeit sowie der An- renewable resources and renewable energies, sustainability passung von Kulturpflanzen an denK limawandel zu erarbeiten. and the adaptation of crop plants to a changing climate. Cor- Entsprechende Forschungsarbeiten schließen sowohl die Nutz- responding research activities include both, the utilisation and barmachung biologischer Vielfalt als auch die Entwicklung und valorisation of plant genetic resources as well as the develop- Anwendung biotechnischer Verfahren ein. ment and deployment of biotechnological approaches.

Stiftungsorgane, Funktionsträger und Boards, Staff with Functional Responsibilities and Organisationsstruktur des IPK Organisational Structure of the IPK Organe der Stiftung sind der Stiftungsrat, das Direktorium The organisational bodies of the Foundation are the Governing und der Wissenschaftliche Beirat. Für die speziellen Belan- Council, the Board of Directors and the Scientific Advisory ge der Bundeszentralen Ex-situ-Genbank steht dem Wissen- Board which is supported by the Genebank Advisory Board. schaftlichen Beirat der Genbank-Beirat zur Seite. Die perso- Members of these bodies are listed on p. 283. In addition, the nelle Zusammensetzung der Beiräte im Berichtsjahr ist in einer list includes all IPK staff members with specific responsibilities. Übersicht auf S. 283 dargestellt. Die Übersicht führt zudem die IPK-Mitarbeiterinnen und Mitarbeiter auf, die mit speziellen Funktionen innerhalb des IPK betraut waren und sind.

6 Das IPK ist in vier wissenschaftliche Abteilungen (Genbank, Cy- The Institute is structured into four scientific departments togenetik und Genomanalyse, Molekulare Genetik, Physiologie (Genebank, Cytogenetics and Genome Analysis, Molecular und Zellbiologie) und die Abteilung Verwaltung und Zentrale Genetics, Physiology and Cell Biology), and the Department of Dienste gegliedert. Die einzelnen Abteilungen untergliedern Administration and Central Services. The departments are fur- sich wiederum in Forschungsbereiche und Arbeitsgruppen (s. ther divided into Programmes (‘Bereiche’) and research groups Organigramm, innere Umschlagseite). Als abteilungsübergrei- (see organisation chart inside back cover). The Plant Genome fende Struktureinheit mit spezieller Aufgabenstellung fungiert Resources Centre (PGRC, p. 151) performs tasks relevant to das Pflanzengenom-Ressourcen-Centrum (PGRC) (s. S. 151). all departments. Regarding Bioinformatics, research and serv- Darüber hinaus werden die Forschungs- und Entwicklungsar- ice activities in the different departments are coordinated via beiten im Bereich Bioinformatik abteilungsübergreifend koor- the IPK Bioinformatics Platform (p. 153). Extramural funding diniert (s. S. 153). Die Einwerbung von Drittmitteln resultiert in contributes substantially to the implementation of the research einer wesentlichen Aufstockung der Personal- und Forschungs- programme (cf. survey on p. 249) mittelausstattung (s. Drittmittelübersicht S. 249 ff). Research Mission Forschungskonzept The strategic development of the research programme is out- Die strategische und inhaltliche Ausrichtung der Forschungs- lined in a budgeted programme that is annually updated. It arbeiten am Institut wird in einer jährlich aktualisierten Pro- presently comprises the following fields: grammplanung festgelegt, die folgende Programme umfasst: 1. Management, Analysis and Evolution of Plant Genetic 1. Management, Analyse und Evolution pflanzengeneti- Resources (Genebank) scher Ressourcen (Abteilung Genbank) 2. Cyto-molecular Genome Analysis (Cytogenetics and Ge- 2. Cyto-molekulare Genomanalyse (Abteilung Cytogenetik nome Analysis) und Genomanalyse) 3. Molecular Physiology of Development (Molecular Genetics) 3. Molekulare Entwicklungsphysiologie (Abteilung Moleku- 4. Applied Physiology and Cell Biology (Physiology and Cell lare Genetik) Biology). 4. Angewandte Physiologie und Zellbiologie (Abteilung Physiologie und Zellbiologie). The topics mirror the complementing research fields of the individual departments. Details are described in the relevant Die Programmthemen spiegeln in wesentlichen Zügen die sich departmental reports. komplementierenden zentralen Arbeitsfelder der Abteilungen wider und werden in den einführenden Abteilungskapiteln The research fields contribute to three priority themes of con- näher spezifiziert. temporary plant research, which are outlined in the strategic research agenda of the IPK as follows: Im Rahmen der genannten, weitgehend disziplinär ausgerich- teten Forschungsprogramme werden Beiträge zu drei großen (a) Conservation and Utilisation of Crop Plant Diversity, Schwerpunkten erarbeitet: which applies the knowledge gained from the analysis of genomes to understand the genetic basis of plant diversity (a) Forschung zur Erschließung, Erhaltung und Nutzung and to create a taxonomic order. The corresponding insights genetischer Diversität, welche in erster Linie die aus der will leverage the use of plant genetic resources. Genomanalyse gewonnenen Erkenntnisse nutzt, um die genetischen Grundlagen der enormen Vielfalt pflanzlicher (b) Dynamics of Plant Genomes, which is increasingly chal- Formen und Leistungen zu verstehen und taxonomisch zu lenging the conventional static picture of the genome and ordnen um daraus Handlungsanweisungen für eine bessere is leading to a realisation of the importance of epigenetic Erhaltung und Nutzung pflanzengenetischer Ressourcen zu processes. This knowledge is becoming of increasing practi- gewinnen; cal significance.

(b) Forschung zur Dynamik pflanzlicher Genome, die das Bild (c) Integrative Biology of Plant Performance, which strives to von der Starrheit der Genome zunehmend ändert und ins- generate a holistic understanding of plants based on multi- besondere die Rolle epigenetischer (bedingt vererbbarer, disciplinary investigation, incorporating a strong compo- nicht auf DNA-Sequenzebene wirkender) Prozesse erhellt, nent of bioinformatics aiming at modelling of metabolic deren Kenntnis mehr und mehr praktische Bedeutung ge- processes, i. e. pursuing a Systems Biology approach. winnt und

7 Das Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)/ The Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

(c) systembiologisch orientierte Forschung zur integrativen Against the backdrop of the large number of species main- Biologie pflanzlicher Leistungen, die aus der Fülle der mit tained by the Genebank research activities of the Institute neuen Methoden gewonnenen Daten versucht, systemati- mainly focus on agriculturally important crop plants. Barley sche Zusammenhänge zu entwickeln, diese zu modellieren (Hordeum vulgare) has been developed as a model system for und daraus ein neues Verständnis des komplexen Systems the Triticeae cereals owing to its agronomic importance and its Pflanze zu gewinnen. elaborate genetics. After having established the technical and bioinformatic infrastructure to perform systematic genome Vor dem Hintergrund der in der Genbank erhaltenen Kultur- research in this species the research portfolio of the Institute pflanzenvielfalt konzentrieren sich die Forschungsarbeiten in has been stepwise expanded to include other priority crops erster Linie auf landwirtschaftlich bedeutsame Pflanzenarten. like wheat, maize and rapeseed. In addition, specific questions Die Arbeiten an Gerste als Modellsystem für die Genomanalyse of seed development are being investigated in legumes. While für Getreide wurden in den vergangenen Jahren auf Weizen, the above mentioned crop species are mostly the target for ap- Mais und Raps ausgeweitet. Nach der erfolgreichen Etablierung plied research, model organisms such as Arabidopsis, Nicotiana einer leistungsfähigen technischen und bioinformatischen and Hypericum are employed to study fundamental questions Infrastruktur für die Genomforschung werden methodenori- in plant biology. In addition, yeast (Arxula adeninivorans) is be- entierte Forschungs- und Entwicklungsansätze zunehmend ing used as a system for research and development in the field durch die Bearbeitung biologischer Fragestellungen ergänzt. of applied biotechnology. Spezielle Fragen zur Samenentwicklung werden seit vielen Jah- ren an Leguminosen untersucht. Die Bearbeitung einer Reihe Integrative Structures and Networks grundlegender Fragestellungen erfolgt in Modellpflanzen wie The Institute has established platforms for internal services and Arabidopsis, Tabak und Hypericum. Ergänzend hierzu werden scientific cross section activities. Beyond the level of individual ausgewählte Forschungsthemen zur angewandten Biotechno- projects IPK is embedded in national and international research logie an Hefe bearbeitet. cooperations, aiming at promoting long-term collaborations, the acquisition of research grants and to attract young scientists. Integrative Strukturen und Netzwerke Das IPK verfügt über abteilungsübergreifende Strukturen, wel- The Plant Genome Resources Centre (PGRC), established in che zum einen als Plattformen für interne Dienstleistungen und 1997, represents a service platform for DNA sequencing and wissenschaftliche Querschnittsaktivitäten dienen. Daneben ist DNA marker analysis (see p. 151). das Institut in über die Projektförderung hinaus gehende nationale und internationale Forschungskooperationen eingebun- Research into Bioinformatics is being performed in close col- den. Diese dienen der Entwicklung und Förderung langfristiger laboration with the experimental groups. In order to warrant a Kooperationsprojekte, der Einwerbung von Projektmitteln und tight interaction of the individual bioinformatics groups both der Gewinnung von wissenschaftlichem Nachwuchs. within the Institute and with the University, the coordinator of the IPK Bioinformatics Platform is jointly appointed with the Das 1997 gegründete Pflanzengenom-Ressourcen-Centrum University of Halle. In the reporting period the research group (PGRC) bildet eine Dienstleistungsplattform für die DNA-Se- “Image Analysis” has been established and now forms an inte- quenzierung und die DNA-Markeranalyse. Weitere Informatio- gral constituent of the Platform (see p. 153). nen zum PGRC finden sich auf Seite 151. The European Barley Genomic Research Network (Barley Die IPK-Bioinformatik-Plattform ist eine koordinative Struk- GenomeNet, BGN) was founded in 2003, and represents a tur, in der alle in den verschiedenen Abteilungen angesiedelten consortium of presently nine institutions from five European Bioinformatik-Gruppen vernetzt sind. Im Berichtszeitraum wur- countries focussing on genome research in barley. BGN part- de der Bereich Bioinformatik durch Etablierung einer Arbeits- ners presently collaborate within several EU projects. Further gruppe zur Bildanalyse verstärkt. Weitere Einzelheiten sind auf information can be retrieved from the BGN website (www.bar- Seite 153 zu finden. leynet.org).

Das 2003 gegründete Europäische Genomforschungs-Netz- In 2006 the International Barley Sequencing Consortium werk Gerste (BarleyGenomeNet – BGN) ist ein Zusammen- (IBSC, http://barleygenome.org) was founded. The consortium schluss von Forschungseinrichtungen, welche sich schwer- presently comprises nine members from seven countries and punktmäßig mit der Genomforschung bei Gerste befassen. aims at generating a full genome sequence of barley based on Neun Einrichtungen aus fünf europäischen Ländern kooperie- a continuously updated research agenda. ren gegenwärtig im Rahmen verschiedener EU-Verbundpro- jekte. Weitere Einzelheiten sind unter www.barleynet.org zu finden.

8 Das “International Barley Sequencing Consortium“ (IBSC, In addition to the platforms and networks mentioned above, http://barleygenome.org) wurde im Dezember 2006 gegrün- there is an extensive network of collaborations within the In- det. Das Ziel ist die Totalsequenzierung des Gerstengenoms. stitute across research groups and departments. Numerous Die Arbeiten basieren auf einer unter den neun Mitgliedsinsti- national and international cooperations are being maintained. tutionen aus sieben Ländern fortwährend abgestimmten For- To further extend ongoing cooperation with the University schungsagenda. of Halle, the University of Kiel and the National Institute for Agrobiological Sciences (NIAS) in Tsukuba, Japan a collabora- Ergänzend zu den genannten Plattformen und Verbünden gibt tion agreement was signed with the Julius Kühn-Institute (JKI), es ein umfangreiches Netzwerk von nationalen und interna- Quedlinburg in December 2010. tionalen Verbundprojekten, sowie arbeitsgruppen- und ab- teilungsübergreifenden IPK-internen Projekt-Kooperationen. Ergänzend zu den bestehenden Kooperationen mit der Martin- Luther-Universität Halle, der Universität Kiel und dem National Institute for Agrobiological Sciences (NIAS) in Tsukuba, Japan, wurde im Dezember 2010 die Zusammenarbeit mit dem Julius Kühn-Institut (Quedlinburg) durch eine Kooperationsvereinba- rung formalisiert und abgesichert.

9 Das Institut in den Jahren 2010 und 2011/ The Institute in 2010 and 2011

Das Institut in den The Institute in Jahren 2010 und 2011 2010 and 2011

Die vergangenen zwei Jahre waren durch eine Fülle inte- The past two years have seen a number of notable research ressanter Forschungsergebnisse, die Weiterentwicklung der outcomes, the ongoing development of the research infra- wissenschaftlich-technischen Infrastruktur, umfangreiche structure involving new build, capital equipment and plant Drittmitteleinwerbung und fortgesetzte nationale und interna- growth facilities, the winning of substantial external funding, tionale Kooperationen gekennzeichnet. Neben der Erhaltung and the cementing of current national and international col- der pflanzengenetischen Ressourcen in der Bundeszentralen laborations. In addition to the conservation of plant genetic Ex-situ-Genbank und den damit verbundenen Dienstleistungs- resources in the IPK Genebank and associated services, the In- aufgaben erstreckten sich die Forschungsaktivitäten von der stitute’s research activities have included topics ranging from Aufklärung grundlegender biologischer Prozesse bis hin zu Be- the analysis of fundamental biological processes to applied arbeitung angewandter Fragestellungen im Vorfeld der Pflan- questions related to crop breeding and biotechnology. In this zenzüchtung und der Biotechnologie. Damit engagiert sich das way, the Institute has involved itself in several of the fields of ac- Institut auf mehreren, der in der nationalen Forschungsstrate- tions outlined in “BioEconomy 2030” (a national strategy of the gie der Bundesregierung „BioÖkonomie 2030“ aufgeführten German Federal Government). An overview of the most impor- Handlungsfeldern und leistet vielfältige Beiträge zur Realisie- tant developments during the reporting period is given below. rung der darin dargelegten Ziele. Nachfolgende Abschnitte More detailed information regarding the research programmes sollen dem Leser einen raschen Überblick zu den wichtigsten can be found in the reports produced by the individual depart- Entwicklungen am IPK vermitteln. Weitere Informationen zu ments and research groups. den Forschungsarbeiten sind den Berichten der Abteilungen und der einzelnen Arbeitsgruppen zu entnehmen. Organizational changes Organisatorische Veränderungen Following the retirement of Bernd Eise at the end of 2009, Sy- Zum 1. Januar 2010 übernahm Sybille-Andrea Lorenz als Nach- bille-Andrea Lorenz became the Institute’s head of administra- folgerin von Bernd Eise die administrative Leitung des IPK. tion.

Am 1. Mai 2010 nahm die Arbeitsgruppe (Ag) Bildanalyse un- The “Image Analysis” research group, led by Dr. Christian Klukas, ter der Leitung von Dr. Christian Klukas ihre Arbeit auf. Die Ar- was established on 1 May 2010. The group’s mandate centres beitsgruppe erarbeitet u. a. neue Auswertealgorithmen für die mainly on the development and implementation of novel algo- Analyse von Bilddaten, die im Zusammenhang mit der automa- rithms required for the analysis of the imaging data generated tisierten Phänotypisierung anfallen. by automated phenotyping platforms.

Seit dem 1. November 2010 wird die durch das Interdisziplinäre The “Stress Genomics” research group was initiated 1 Novem- Zentrum für Nutzpflanzenforschung finanzierte Nachwuchs- ber 2010, led by Dr. Nese Sreenivasulu and funded by the Inter- gruppe Stress-Genomik von Dr. Nese Sreenivasulu geleitet. disciplinary Centre of Crop Plant Research at the Martin Luther Hauptgegenstand der Forschungsarbeiten ist die Aufklärung University, Halle. Its focus lies in the analysis of regulatory gene von Genregulationsnetzwerken sowie der Rolle des Pflanzen- networks and the role of the phytohormone abscisic acid in the hormons Abscisinsäure (ABA) bei der Anpassung von Getreide adaptation of plants to drought stress. an Trockenstress. Prof. Anna Wobus, head of the “In vitro Differentiation” research Ende 2010 ging die Leiterin der Ag In-vitro-Differenzierung, group retired at the end of 2010, and the group was discontin- Prof. Dr. Anna Wobus, in den Ruhestand. Ein Teil der Forschungs- ued. Part of its research programme has been transferred to the arbeiten zu embryonalen Stammzellen wird an der Martin- Martin Luther University, Halle. Luther-Universität in Halle weitergeführt. In August 2011 the “Expression Analysis” research group led by Im August 2011 wurde die Ag Expressionsanalyse (Leiter Dr. Dr. Lothar Altschmied was incorporated into the “Heterosis” re- Lothar Altschmied) in die Ag Heterosis (Leiter: Prof. Dr. Thomas search group, headed by Prof. Thomas Altmann. Altmann) integriert. On 1 January 2012 the “Taxonomy of Plant Genetic Resources” Zum 1. Januar 2012 wurde die Ag Taxonomie Pflanzengeneti- research group was merged with the “Experimental Taxonomy” scher Ressourcen in die Ag Experimentelle Taxonomie (Leiter research group headed by Dr. Frank Blattner. Dr. Frank Blattner) überführt.

10 Entwicklungen von zentraler Bedeutung Developments of particular importance (1) Die Einwerbung von Drittmitteln stellt eine wichtige (1) the winning of external funding has long been seen as es- Grundlage für die Umsetzung des Forschungsprogramms sential for the implementation of the Institute’s research dar. Im Berichtszeitraum hat sich das Institut in verschiede- programme. The Institute has been successful in acquiring nen Förderprogrammen, allen voran in der vom BMBF auf- such support from a range of agencies, in particular the gelegten Förderlinie „Pflanzenbiotechnologie der Zukunft“, BMBF “Plant Biotechnology of the Future” programme. An- erfolgreich um die Einwerbung von Drittmitteln bemüht. other notable donor is the Leibniz Association, which has Daneben konnten im Jahr 2011 Fördermittel aus dem WGL helped establish a graduate school under the theme “Yield Pakt für Forschung und Innovation für den Aufbau einer formation in cereals – overcoming yield-limiting factors”, Graduiertenschule zum Thema „Yield formation in cereals due to be launched in 2012. Financial support from the Ger- – overcoming yield-limiting factors“ eingeworben werden. man Research Council (DFG) has increased substantially, in Erfreulich ist auch der Anstieg von eingeworbenen Förder- recognition of the national significance of the fundamental mitteln der DFG. Diese stellen ein wichtiges Exzellenzkrite- research programme carried out at IPK. Thanks to the main- rium dar. Der guten Drittmittelsituation entsprechend wa- tenance of a high level of external funding, the number of ren im Berichtszeitraum durchgängig über 500 Personen employees at IPK has remained above 500 throughout the am Institut beschäftigt. reporting period.

(2) Forschungsergebnisse wurden in den vergangenen beiden (2) iPK’s research outcomes over the past two years have re- Jahren in 306 Artikeln in referierten Fachzeitschriften veröf- sulted in the publication of 306 peer-reviewed papers and fentlicht. Hinzu kommen 46 Publikationen, die als Buchbei- 46 book chapters. On the education side, as part of the In- träge erschienen sind. stitute’s formal arrangements with the Martin Luther Uni- im Zuge der Ausbildung von wissenschaftlichem Nach- versity, Halle, the period has seen the completion of ten wuchs wurden im Berichtszeitraum 20 Bachelor-, 14 Di- BSc, 14 Diploma, seven MSc and 31 PhD theses, along with plom- und 7 Masterarbeiten sowie 31 Dissertationen und one habilitation. Details of the respective thesis topics are eine Habilitation abgeschlossen. Eine detaillierte Aufstel- given in the reports of the individual research groups. lung bzw. Zuordnung ist in den Berichten der einzelnen Arbeitsgruppen zu finden. (3) a continuing programme of extension and modernization to the stock of greenhouses at the site has been carried out (3) Der Ausbau der Gewächshausanlagen stellt eine wichtige to support the focus on phenotypic analysis via automated Voraussetzung für die Erfassung pflanzlicher Merkmale mit imaging. The pre-existing system designed to phenotype Hilfe moderner bildgebender Verfahren dar. Daher wurde cereal plants has been strengthened by the addition of a das vorhandene Gewächshaus zur automatischen Phänoty- second system able to handle up to 1,600 pots containing pisierung von Getreide mit der Inbetriebnahme einer weite- plants as tall as 2.50 m. The growth chamber area was ex- ren Anlage, die auf maximal 1600 Töpfe ausgelegt ist und in tended by 80 m², incorporating an automated phenotyp- der Pflanzen bis zu einer Wuchshöhe von 2,50 m angezogen ing unit targeting Arabidopsis thaliana plants. werden können, ergänzt. Parallel hierzu erfolgte die Inbe- triebnahme einer ebenfalls mit einer automatischen Phäno- (4) the Genebank and the Administration and Central Services typisierungseinheit ausgestatteten Klimakammer. Departments successfully underwent an external audit in 2010 and 2011, leading to their being awarded DIN EN ISO (4) im Rahmen von zwei externen Audits in den Jahren 2010 9001:2008 quality management certification. und 2011 wurde der Zertifizierungsstatus des Qualitätsma- nagements nach DIN EN ISO 9001:2008 der Genbank und (5) in December 2010, IPK signed a bilateral agreement with der Abteilung Verwaltung und Zentrale Dienste bis 2013 the Julius Kühn Institute, Quedlinburg, to formalize and bestätigt. further extend current collaborations between the two institutions. In spring 2011, the relationship with the Martin (5) im Dezember 2010 wurde ein Kooperationsabkommen mit Luther University, Halle was strengthened by the establish- dem Julius Kühn-Institut (JKI) in Quedlinburg zur Formali- ment of the “Leibniz Science Campus”, which has brought sierung und Intensivierung der wissenschaftlichen Zusam- together two of the University’s faculties, IPK and two other menarbeit der beiden Einrichtungen unterzeichnet. Leibniz Institutes (the Leibniz Institute of Plant Biochemis- Die Zusammenarbeit mit der Martin-Luther-Universität Hal- try and the Leibniz Institute of Agricultural Development in le wurde weiter ausgebaut. Gemeinsam mit dem Leibniz- Central and Eastern Europe). The mandate of this consor- Institut für Pflanzenbiochemie (IPB) und dem Leibniz-Insti- tium is to develop a research programme along the theme tut für Agrarentwicklung in Mittel- und Osteuropa (IAMO) “the plant-based bioeconomy”. erfolgte im Frühjahr 2011 die Gründung des Leibniz-Wis- senschaftscampus für „Pflanzenbasierte Bioökonomie“. Die Anschubfinanzierung erfolgt aus Mitteln des Landes und der Leibniz-Gemeinschaft.

11 Das Institut in den Jahren 2010 und 2011/ The Institute in 2010 and 2011

Abb. 1 Die Gewinner der Forschungspreise des Landes Sachsen-Anhalt 2011: Prof. Dr. Niels Olaf Angermüller (l.; Hochschule Harz, Wernigerode), Dr. Daniela C. Dieterich (Mitte; Leibniz-Institut für Neurobiologie, Magdeburg) und Prof. Dr. Gotthard Kunze. (r., IPK Gatersleben; Foto: H. Ernst/IPK Gatersleben) Fig. 1 The award winners of the Research Awards of Saxony-Anhalt 2011: Prof. Niels Olaf Angermüller (University of Applied Sciences Harz, Wernigerode), Dr. Daniela C. Dieterich (Leibniz Institute for Neurobiology, Magdeburg) and Prof. Gotthard Kunze (IPK, Gatersleben), (l. to r.; Photo: H. Ernst/IPK Gatersleben).

(6) auch im Berichtszeitraum wurde eine Reihe von Forschern (6) During the current reporting period, a number of IPK re- des Instituts mit Preisen ausgezeichnet. Stellvertretend sei searchers received recognition in the form of awards. The an dieser Stelle Dr. Nils Stein aufgeführt, der im März 2010 most prominent were the “Günter und Anna Wricke” prize den Günter und Anna Wricke Preis für seine Arbeiten zur given to Dr. Nils Stein in March 2010 for his research on Genomforschung und anwendungsorientierten Genetik genome analysis and applied genetics in cereals, and the bei Getreide (Triticeae) erhielt. Im Dezember 2011 wurde “Applied Research Award of Saxony-Anhalt” awarded to Prof. Dr. Gotthard Kunze für seine Arbeiten auf dem Ge- Prof. Gotthard Kunze in December 2011 in recognition of his biet der Hefebiotechnologie mit dem Forschungspreis des contribution to the field of yeast biotechnology (Fig. 1). In Landes Sachsen-Anhalt für angewandte Forschung ausge- September 2011, Prof. Anna and Prof. Ulrich Wobus, two re- zeichnet (Abb. 1). cently retired IPK staff who guided the development of the Mit Prof. Dr. Anna und Prof. Dr. Ulrich Wobus wurden im Institute over many years, received the prestigious Cothe- September 2011 zwei herausragende, ehemalige Mitar- nius Medal of the German Academy of Sciences Leopoldina beiter des Instituts, deren Namen untrennbar mit der Ent- in honour of their outstanding scientific achievements. wicklung des IPK Gatersleben verbunden sind, von der Deutschen Akademie für Naturforscher Leopoldina für ihr wissenschaftliches Lebenswerk mit der Cothenius-Medaille Activities of the Advisory Boards gewürdigt. As in previous years, the Scientific Advisory Board and the Genebank Advisory Board convened after the Institute Day. In 2010 (4-6 October), the Scientific Advisory Board reviewed Die Arbeit der Gremien the Genebank and the Cytogenetics and Genome Analysis De- Wie in den vergangenen Jahren trafen sich der Wissenschaft- partments, whereas in 2011 (4-6 October), the research groups liche Beirat und der Genbank-Beirat anlässlich des Instituts- housed within the Molecular Genetics Department and the tags zur ihrer Begutachtung. Im Jahr 2010 (4. bis 6. Oktober) Physiology and Cell Biology Department were evaluated. In par- befasste sich der Wissenschaftliche Beirat mit den Abteilungen allel, the Genebank Advisory Board visited the Genebank in both

12 Abb. 2 Die Teilnehmer der 10. Gatersleben Research Conference „Sequence-informed Crop Research“ Ende November 2010. (Foto: R. Schnee/ IPK Gatersleben) Fig. 2 Participants of the 10th Gatersleben Research Conference “Sequence-informed Crop Research“ late November 2010. (Photo: R. Schnee/ IPK Gatersleben)

Genbank sowie Cytogenetik und Genomanalyse, im Jahr 2011 years. (4. bis 6. Oktober) wurden die Forschungsarbeiten in den Abtei- The Governing Board convened in both 2010 and 2011 on lungen Molekulare Genetik sowie Physiologie und Zellbiologie 7 October under the chairmanship of Dr. Joachim Welz from begutachtet. Parallel hierzu besuchte der Genbank-Beirat in the Ministry of Science and Economics of Saxony-Anhalt. The beiden Jahren die Genbank. topics for consultation included programme budgeting, infrastructure on the campus, the acquisition of capital equipment, Der Stiftungsrat tagte in beiden Jahren jeweils am 7. Oktober and the succession for head of the Cytogenetics and Genome unter der Leitung des Vorsitzenden, MinDirig Dr. Joachim Welz. Analysis Department. Im Mittelpunkt der Besprechungen standen die Fortführung des Programmbudgets, Planungen zum weiteren Ausbau der Gewächshaus- und Klimakammerkapazitäten, die Beschaffung Conferences and Workshops von Großgeräten, sowie der im Jahr 2012 anstehenden Nach- In 2010 and 2011, IPK scientists were engaged in the organi- besetzung der Stelle des Leiters der Abteilung Cytogenetik und zation of a number of both national and international confer- Genomanalyse. ences and workshops. The 10th Gatersleben Research Confer- ence with the theme Sequence-informed Crop Research took place on 22-24 November 2010, attracting over 160 delegates Tagungen und Workshops interested in the current state of plant genome research (Fig. 2). Im Berichtszeitraum waren Forscher des IPK an der Ausrichtung From 20-24 September 2010, 32 junior researchers from all over zahlreicher nationaler und internationaler Tagungen, Symposi- Europe gathered to participate in the European Science Foun- en und Workshops beteiligt. 2010 veranstaltete das IPK vom dation (ESF)-funded “Plant Epigenetics 2010” Summer School. 22. bis 24. November die 10. Gatersleben Research Conference The scientific programme comprised invited lectures from in- zum Thema „Sequence-informed Crop Research” (Abb. 2). Über ternationally renowned (plant) epigeneticists and fields com- 160 Wissenschaftler diskutierten am IPK rezente Entwicklun- plementary to epigenetics, and a series of laboratory exercises gen und Ergebnisse aus dem Bereich der pflanzlichen Genom- was also included. forschung. Vom 20. bis 24. September des gleichen Jahres wurde eine von der European Science Foundation (ESF) geförderte IPK also continued its ongoing discourse with other scientific Summer School „Plant Epigenetics 2010“ ausgerichtet, an der disciplines and the public. From 12-14 May 2011, about 90 par- 32 Nachwuchswissenschaftler/-innen aus ganz Europa teilnah- ticipants of the 12. Gaterslebener Begegnung (12th Gatersle- men. Das Programm der Summer School beinhaltete sowohl ben Meeting) discussed the topic “Reifung und Wachstum in theoretische Wissensvermittlung in Form von eingeladenen Natur und Gesellschaft” (Maturation and growth in nature and Vorträgen international anerkannter Epigenetiker oder von society) which had particular resonance in the light of the on- Wissenschaftlern aus angrenzenden Feldern als auch Labor- going turmoil affecting the financial markets. The conference Kurse. was jointly organised with the German Academy of Sciences Leopoldina (see Fig. 3, p. 14). From 31 October to 3 November 2011 IPK hosted a workshop funded jointly by DFG and the

13 Das Institut in den Jahren 2010 und 2011/ The Institute in 2010 and 2011

Abb. 3 Prof. Dr. Ulrich Wobus und Prof. Dr. Anna M. Wobus engagieren sich seit Jahren in der Organisation und Durchführung der Gaterslebener Begegnungen. (Foto: H. Ernst/ IPK Gatersleben) Fig. 3 The ‘inventors’ of the ‘Gaterslebener Begegnungen’ (Gatersleben Meetings): Profs. Ulrich and Anna M. Wobus. (Photo: H. Ernst/ IPK Gatersleben)

Vom 12. bis 14. Mai 2011 begrüßte das IPK die etwa 90 Japanese Society for the Promotion of Sciences on the topic Teilnehmer/-innen der 12. Gaterslebener Begegnung zum ‘Frontiers of Plant Chromosome Research: Centromeres and Thema „Reifung und Wachstum in Natur und Gesellschaft“, die Artificial Chromosomes’. The focus of this meeting was to en- wiederum gemeinsam mit der Leopoldina ausgerichtet wurde courage German and Japanese scientists to exchange recent (Abb. 3). Mit Vorträgen von Publizisten und Wissenschaftlern achievements in chromosome biology and to intensify bilateral wurde die Thematik im Lichte der aktuellen Entwicklungen an contacts. The members of the Student Board organized the 6th den Finanzmärkten insbesondere im Hinblick sozial- und wirt- Plant Science Students’ Conference from 15-18 June 2010 at schaftspolitischer Gesichtspunkte erörtert. IPK. An overview of all the conferences and symposia held at Vom 31. Oktober bis zum 3. November 2011 war das IPK Gast- IPK, as well as a list of the external conferences whose organ- geber für einen von der DFG und der Japanischen Society for izers included IPK scientists is given on page 208. the Promotion of Sciences (JSPS) finanzierten Workshops zu „Frontiers of Plant Chromosome Research: Centromeres and Artificial Chromosomes“. An der Veranstaltung nahmen vor- Cooperation with Universities and Research Institutes rangig Wissenschaftler aus Deutschland und Japan teil, um die Interactions at the institutional level are an important compo- Kommunikation in der Chromosomenbiologie zu intensivieren nent of the development of IPK’s research agenda, since these und die jeweils letzten Forschungsergebnisse zu präsentieren. take advantage of complementarities in scientific know-how and technical infrastructure. Close interactions between the In- Das IPK Student Board veranstaltete vom 15. bis 18. Juni 2010 stitute and neighbouring universities are expected to increase die 6. Plant Science Student Conference am IPK, die im Wech- the attractiveness of IPK as a destination for young academics. sel mit der Doktorandenorganisation des IPB in Halle seit 2005 In December 2010, an agreement was signed with the Julius jährlich stattfindet. Kühn Institute to formalize and promote the scientific collaborations enjoyed by staff of the two institutes. The “Leibniz Eine Übersicht zu allen am IPK veranstalteten Symposien und Science Campus” was founded in 2011 as a means of deepening der Beteiligung an der Ausrichtung externer Tagungen und the already well established relationship between the Martin Symposien in 2010 und 2011 findet sich auf S. 208 ff. Luther University, Halle and its neighbouring Leibniz Institutes. The hoped-for outcome is that the campus will evolve into an interdisciplinary research platform addressing the plant-based bioeconomy, involving participants from both the life and the social sciences. Start-up funding was provided by the State of Saxony-Anhalt and the Leibniz Association (Fig. 4, p. 15).

14 Abb. 4 Unterzeichnung der Kooperationsvereinbarung am 4. März 2011: Die Kultusministerin, Frau Prof. Birgitta Wolff, und der Präsident der Leibniz-Gemeinschaft, Prof.K arl Ulrich Mayer, unterzeichnen die Vereinbarung zum WissenschaftsCampus. (Foto: R. Honeit/ IAMO Halle) Fig. 4 Signing the formal contracts for the inauguration of the Leibniz Science Campus: Minister of Cultural Affairs of Saxony-Anhalt, Prof. Birgitta Wolff and President of the Leibniz Association, Prof. Karl Ulrich Mayer. (Photo: R. Honeit/ IAMO Halle)

Zusammenarbeit mit Universitäten und IPK scientists have given tuition at the Universities of Halle-Wit- Forschungseinrichtungen tenberg, Potsdam, Kassel, Kiel, Jena, Greifswald, Western Aus- Ein wichtiger Aspekt im Hinblick auf die Weiterentwicklung tralia (Perth), and the University of Applied Sciences in Bern- des Forschungsprogramms ist die Vernetzung mit Forschungs- burg and Köthen. In addition, our scientists have given several einrichtungen. Hierbei sollen vor allem Komplementäreffekte courses and run a number of workshops for the Institute’s PhD (Know-how, technische Ausstattung) genutzt werden. Durch student community. The size of the community reached 88 die enge Verbindung des Instituts mit den umliegenden Uni- students by the end of 2011. IPK’s PhD programme has been versitäten und Hochschulen soll, unter anderem, auch die At- actively coordinated by the “PhD Student Board”, which organ- traktivität des IPK als Ausbildungsstätte für den wissenschaftli- ized a student seminar series, hosted invited lectures given by chen Nachwuchs gesteigert werden. external scientists, staged a “Plant Science Students’ Confer- ence” and provided various training events for the PhD student Im Dezember 2010 wurde ein Kooperationsabkommen mit community. Thanks to a grant from the Leibniz Association, a dem Julius Kühn-Institut (JKI) in Quedlinburg zur Formalisie- graduate school themed “Yield formation in cereals – overcom- rung und Intensivierung der wissenschaftlichen Zusammen- ing yield-limiting factors” will be established in 2012, together arbeit der beiden Einrichtungen unterzeichnet. Im Zuge der with the Martin Luther University, Halle. Further details of the weiteren Intensivierung der Zusammenarbeit der Martin- IPK PhD programme can be found on page 155. Luther-Universität Halle mit den in der Region angesiedelten außeruniversitären Forschungseinrichtungen wurde 2011 der „Leibniz-WissenschaftsCampus“ gegründet (Abb. 4). In diesem sollen Forschungsarbeiten zur pflanzlichen Bioökonomie diszi- plinübergreifend durch die Zusammenführung von lebenswis- senschaftlichen und wirtschaftswissenschaftlichen Ansätzen bearbeitet werden.

15 Das Institut in den Jahren 2010 und 2011/ The Institute in 2010 and 2011

Wissenschaftler des Instituts führten Lehrveranstaltungen an Public relations and public image oder in Zusammenarbeit mit den Universitäten Halle-Witten- The recognition of IPK within the scientific community is shaped berg, Potsdam, Kassel, Kiel, Jena, Magdeburg und Greifswald by its output of publications in international peer-reviewed sci- sowie der Hochschule Anhalt an den Standorten Bernburg und entific journals along with the talks and poster presentations Köthen fort. Daneben boten Wissenschaftler Kurse und Prakti- made by the Institute’s staff at national and international con- ka für Studierende am IPK an. Die Anzahl der am IPK betreuten ferences. The visibility of IPK to the general public, in contrast, Doktoranden wurde erheblich gesteigert. Ende 2011 waren 88 has to rely on contributions in the print, TV, radio and online Doktoranden/-innen am IPK tätig. Das am IPK etablierte Dok- media. This avenue of communication can be supplemented torandenprogramm wird über weite Strecken durch das “PhD more locally by organizing open campus tours. Over the past Student Board“ entwickelt und betreut (s. S. 155). Zu den Akti- two years, around 1,200 visitors have toured the Gatersleben vitäten zählen die Veranstaltungen in der Reihe der Doktoran- headquarters and the two Genebank satellite stations in Groß denseminare, Einladungen von externen Wissenschaftlern zu Lüsewitz and Malchow/Poel. The same period has seen 20 press Seminarvorträgen, die Organisation der “Plant Science Student releases aimed to inform the public and media concerning re- Conference“ sowie spezielle Fortbildungsveranstaltungen für cent developments at the Institute and the Biotech Campus. Doktoranden. Im Zuge der weiteren Strukturierung des Dokto- A number of IPK researchers took the opportunity to provide randenprogramms wird ab 2012 gemeinsam mit der MLU Halle first-hand information to press and media representatives con- eine Graduierten-Schule zum Thema „Yield formation in cereals – cerning a variety of scientific issues. Most of the interest has overcoming yield-limiting factors“ anlaufen, welche aus Mitteln centred on the activity of the Genebank (biodiversity issues) des Leibniz-Pakts für Forschung und Innovation gefördert wird. and green biotechnology. Together with its campus partners, IPK organised an Open Day in both 2010 and 2011. In 2010, the Öffentlichkeitsarbeit und öffentliche Wirkung Open Day was extended by providing a “Fest der Begegnung” Die Wahrnehmung des IPK innerhalb der wissenschaftlichen (“Visitors’ festival”) which was well-received by the public. Gemeinde basiert zum größten Teil auf den oben aufgeführten wissenschaftlichen Publikationen. Ebenso stellen Vorträge auf IPK also had a presence at various international fairs, in particu- internationalen Konferenzen bzw. Posterbeiträge (vgl. S. 165 ff.) lar Biotechnica in both 2010 and 2011. Two exhibits (“A biosen- einen wichtigen Baustein in der Kommunikation mit der For- sor designed to detect the presence of oestrogenic compounds schergemeinschaft dar. in water samples” and “A DNA-based sensor able to detect the colonization of plant roots by Mycorrhiza”) displayed on the Für die Darstellung in der breiten Öffentlichkeit spielen Medi- joint (Saxony, Thuringia and Saxony-Anhalt) stand “Research en- und Pressearbeit sowie Führungen von Besuchergruppen for the Future” featured outcomes of IPK’s biotechnology re- durch die Standorte des IPK eine besonders große Rolle. So search. IPK scientists also presented talks at the BMBF innova- konnten in den beiden Jahren über 1200 Besuchern während tion pavilion. The “A biosensor designed to detect the presence 71 Führungen durch das Institut in Gatersleben und die beiden of oestrogenic compounds in water samples” exhibit was also Standorte der Genbank in Groß Lüsewitz und Malchow/Poel In- displayed at the Analytica 2010 trade show in Munich. formationen zum Institut und zur Pflanzenforschung allgemein vermittelt werden. Ein erheblicher Anteil der Führungen wurde On 4 October 2010, the “Gatersleben Research Award” was – resultierend aus dem öffentlichen Interesse – von den Grup- granted to Dr. Navreet Kaur (University of Zurich) for her doc- pen der Genbank in Gatersleben und an den beiden Standor- toral thesis on the topic of resistance genes against powdery ten Groß Lüsewitz und Malchow/Poel übernommen. mildew in wheat.

Daneben wurde die Öffentlichkeit im Berichtszeitraum mit 20 Last, but not least, IPK continued its support of the activities Presseinformationen über aktuelle Entwicklungen am Institut of the “Gesellschaft zur Förderung der Kultur Gatersleben e.V.” und auf dem Biotech-Campus informiert. In diesem Zusam- (The Gatersleben Society for Cultural Progress) which cel- menhang standen Mitarbeiter des Instituts Medienvertretern ebrated its twentieth anniversary in May 2011. für wissenschaftliche Fragestellungen bzw. zu am IPK erzielten Forschungsergebnissen zur Verfügung. Seitens der Medien wa- A comprehensive overview of the Institute’s various PR activi- ren das Thema Grüne Gentechnik sowie die Genbank von be- ties is given on pages 231-248. sonderem Interesse.

Gemeinsam mit den Partnern vor Ort veranstaltete das IPK sowohl in 2010 als auch in 2011 einen Tag der offenen Tür, wel- cher 2010 mit dem Fest der Begegnung fortgesetzt wurde. Letzteres erfreut sich in der Region großer Beliebtheit, da u. a. die ausländischen Mitarbeiter spezielle Angebote aus ihren Heimatländern bereit halten.

16 Auf der Biotechnica 2010 und 2011 in Hannover war das Institut Gatersleben as a “Centre for Green Biotechnology” mit Exponaten zu den Themen „Biosensor zum Nachweis östro- As a scientific centre, the Institute is variously engaged in the genwirksamer Substanzen im Wasser“ und „DNA-Sensor zum local initiative “Green Gate Gatersleben”, and supports the de- Nachweis mykorrhizierter Pflanzen“ auf dem Gemeinschafts- velopment of the Biotechnology Campus (http://www.green- stand „Forschung für die Zukunft“ der Länder Sachsen, Sach- gate-gatersleben.de). Collaboration with private companies sen-Anhalt und Thüringen vertreten. Darüber hinaus hielten which share the campus is primarily conducted in the frame Forscher des IPK Vorträge z. B. auf dem Innovations-Forum des of joint research projects. The development of the Gatersleben BMBF. Ebenso stellte das IPK auf der Analytica 2010 in München Campus as a biotechnology hub is constrained by the opposi- das Exponat „Biosensor zum Nachweis östrogenwirksamer Sub- tion to GM technology which continues to be a feature of public stanzen im Wasser“ aus. Zur Vorbereitung dieser Messen und and political opinion. A damaging result of this opposition has anderer Veranstaltungen führte das Institut seine Mitarbeit im been a stalling in the establishment of a workable legal frame- Arbeitskreis „Messe“ des Landes Sachsen-Anhalt fort. work to regulate the co-existence of GM and non-GM plants. Against this backdrop, the decision of Bayer CropScience to es- Im Rahmen des Institutstages wurde am 4. Oktober 2010 der tablish its European headquarters for wheat breeding on the durch die „Gemeinschaft zur Förderung der Kulturpflanzenfor- Gatersleben Biotechpark was particularly welcome. schung e.V.“ und das IPK gestiftete Gaterslebener Forschungs- preis an Frau Dr. Navreet Kaur Bhullar von der Universität Zürich To spur interest in life sciences amongst the young, the “Grünes für ihre Dissertation zur Entdeckung von neuen Resistenzgenen Labor” (“Green laboratory”) has worked to develop an extensive gegen Weizenmehltau verliehen. curriculum. This has attracted an ever-increasing level of interest, as shown by the enrolment of over 7,000 people (mainly Neben den wissenschaftlichen Arbeiten unterstützt das IPK ak- school-age children) in the various courses and events organ- tiv die kulturellen Aktivitäten der „Gesellschaft zur Förderung ized over the past two years. der Kultur Gatersleben e.V.“, die im Mai 2011 ihr 20-jähriges Be- stehen feierte.

Eine Übersicht zu allen Öffentlichkeitsarbeit-relevanten Aktivi- täten findet sich auf den Seiten 231-248.

Der Biotechnologiestandort Gatersleben Als wissenschaftliches Zentrum ist das Institut auf vielfältige Weise an der Standortinitiative „Green Gate Gatersleben“ beteiligt und unterstützt die Weiterentwicklung des Biotechnologie- standorts (http://www.green-gate-gatersleben.de). Die Zusam- menarbeit mit verschiedenen am Standort angesiedelten Fir- men erfolgt in erster Linie durch die Bearbeitung gemeinsamer Forschungsprojekte. Die Ablehnung der Grünen Gentechnik in weiten Teilen der Gesellschaft und Politik sowie die unzuläng- lichen gesetzlichen Rahmenbedingungen, die einer Koexis- tenz zwischen gentechnisch veränderten und konventionellen Pflanzen entgegen stehen, erschweren denE rhalt bzw. die Wei- terentwicklung des Biotechnologiestandorts Gatersleben erheblich. Umso erfreulicher war daher die Ansiedlung der Firma Bayer CropScience im Biotechpark, wo im Dezember 2011 mit dem Aufbau des europäischen Zentrums für Weizenzüchtung begonnen wurde.

Um das Interesse Jugendlicher an Lebenswissenschaften zu wecken, bietet das auf dem Campus angesiedelte „Grüne La- bor“ ein breites Kursangebot. Dieses zielt in erster Linie auf den schulischen Bereich ab, wurde in den vergangenen zwei Jahren jedoch auch auf die berufliche Ausbildung ausgeweitet. Das Angebot stieß weiterhin auf sehr großes Interesse. Etwa 7.000 Schüler, Auszubildende und Lehrer nahmen in den vergangenen beiden Jahren an den Kursen und Veranstaltungen teil.

17 Verwaltung und Infrastruktur/Administration and Infrastructure

Verwaltung und Infrastruktur/ Administration and Infrastructure

Personal/Staff Budget In den beiden Berichtsjahren 2010 und 2011 betrug der Ge- Seit der Einführung der Programmbudgets an Stelle der Wirt- samtpersonalbestand (zum jeweiligen Stichtag: 31. Dezember) schaftspläne werden die Einnahmen und Ausgaben für die Pro- 535 (2010) bzw. 550 (2011) Personen. Darunter befanden sich gramme ungeachtet der Mittelherkunft betrachtet. Dem IPK am 31. Dezember 2011 275 (2010: 272) Mitarbeiter/-innen auf standen 2011 insgesamt 42.136 TEUR (2010: 42.144 TEUR) für Planstellen. Neben 155 (2010: 161) Drittmittelbeschäftigten eigene Ausgaben, d. h. ohne Einnahmen für Partner und ohne waren 96 (2010: 81) Mitarbeiter/-innen über Annexstellen an- Einbehalte für Baumaßnahmen, zur Verfügung. gestellt. (Abb. 5, S. 19) In 2011 betrugen die Zuwendungen im Rahmen der Grundfi- nanzierung 24.575 TEUR (2010: 24.547 TEUR). Darin enthalten Auch im Bereich der Berufsausbildung hat das Institut mit ins- waren 922 TEUR Betriebsmittel für die Projekte im Rahmen des gesamt 24 (2010: 21) Ausbildungsplätzen in verschiedenen wettbewerblichen Verfahrens der Leibniz-Gemeinschaft (SAW) Berufen und einem dualen Studenten das hohe Niveau halten (2010: 682 TEUR) (Abb. 8, S. 21). können. Darunter drei Bürokaufleute, 11 Biologielaborant(en)/- innen, drei Fachinformatiker Systemintegration, eine Fachan- Etwa 56 % der Gesamtausgaben entfielen auf den Personal- gestellte für Medien- und Informationsdienste, drei Gärtner/- bereich. Der Anstieg bei den Betriebsausgaben war vorrangig innen für Gemüsebau und erstmalig ab 2010 zwei Köche im auf die Tariferhöhung im TV-L sowie auf die Kostensteigerung Casino des IPK. bei den Bewirtschaftungskosten zurückzuführen. Die Bauin- vestitionen und die aus dem Konjunkturprogramm II der Bun- Zum 30. September 2011 waren 339 Personen in einem befris- desregierung (KP II) finanzierten Maßnahmen wurden 2010 teten Arbeitsverhältnis tätig. Von den 183 Wissenschaftler/- abgeschlossen. Ferner erhielt das IPK im Rahmen des Gesetzes innen waren insgesamt 151 befristet beschäftigt. Von den 61 zur Umsetzung von Zukunftsinvestitionen der Kommunen im Planstellenbereich beschäftigten Wissenschaftler/-innen und Länder/ Konjunkturpaket II Mittel für Geräteinvestitionen, hatten 29 eine befristete Anstellung. Die Abteilungen Genbank die 2010 in einem Umfang von 2.130 TEUR eingesetzt wurden und Verwaltung und Zentrale Dienste verfügen über einen re- (Abb. 9, S. 21). lativ hohen Anteil an Stellenplanpersonal im Vergleich zu den anderen drei wissenschaftlichen Abteilungen (Abb. 6, S. 19). Wissenschaftliche und technische Mitarbeiter der Genbank Drittmitteleinnahmen/Third Party Funding nehmen Daueraufgaben bei der Erhaltung und dem Manage- Im Jahr 2011 wurden für 148 Projekte (2010: 150) Einnah- ment der in der Bundeszentralen Ex-situ-Genbank bewahrten men (ohne Partner) in Höhe von insgesamt 10.623 teuR Vielfalt wahr. Die Mitarbeiter der Abteilung Verwaltung und (2010: 10.323 TEUR) erzielt (Abb. 10, S. 21). Hauptzuwendungs- Zentrale Dienste unterstützen im gärtnerischen und techni- geber waren das Bundesministerium für Bildung und For- schen Bereich ganz unmittelbar die Forschungsarbeiten in den schung (BMBF), die Deutsche Forschungsgemeinschaft (DFG) vier wissenschaftlichen Abteilungen. und das Land Sachsen-Anhalt. Außerdem erhielt das IPK Mit- tel von sonstigen Zuwendungsgebern in Höhe von 272 TEUR Gb abteilung Genbank (Vorjahr 298 TEUR). Neben den Einnahmen für das IPK wurden CYG abteilung Cytogenetik und Genomanalyse 37 TEUR (Vorjahr 255 TEUR) für Partner eingenommen und wei- MOG abteilung Molekulare Genetik tergereicht. Pzb abteilung Physiologie und Zellbiologie VZD abteilung Verwaltung und Zentrale Dienste Die Einnahmen vom BMBF resultierten überwiegend aus dem inzwischen abgeschlossenen Programm „GABI-FUTURE“ sowie Ein relativ hoher Personalanteil des Institutes ist im technischen aus der neuen Förderinitiative „Pflanzenbiotechnologie derZ u- und infrastrukturellen Bereich tätig (Abb. 7, S. 19). Dies ist wie kunft“. Der absehbare Rückgang bei den durch das BMBF geför- bereits aufgeführt auf den großen gärtnerischen und techni- derten Projekten konnte sowohl durch DFG-Mittel als auch Auf- schen Betreuungsaufwand in der Pflanzenforschung bzw. im tragsforschung für Wirtschaftsunternehmen mehr als ausgegli- Vermehrungsanbau der Genbank zurückzuführen. chen werden. Die Zusammensetzung der Drittmittelherkunft zeigt, dass die Umsetzung des Leibniz-Prinzips ‑ exzellente Wis- senschaft mit Anwendungsrelevanz ‑ zu einem ausgewogenen Verhältnis zwischen Grundlagenforschung und angewandter Forschung am IPK führt.

18 Abb. 5 Personalentwicklung (Stand: 30. September 2011) Fig. 5 Development of staff (as of 30th September 2011)

Abb. 6 Beschäftigte nach Abteilungen (Stand: 30. September 2011) Fig. 6 Staff per department (as of 30th September 2011)

Abb. 7 Personalstruktur (Stand 30. September 2011) Fig. 7 Personnel structure (as of 30th September 2011)

19 Verwaltung und Infrastruktur/Administration and Infrastructure

Technologietransfer/Technology Transfer Wissenschaftliche Bibliothek/Scientific Library Im Jahr 2011 wurden 36 Erfindungen (2010: 5) durch Wissen- Die Wissenschaftliche Bibliothek des IPK verfügt über einen schaftler des IPK gemeldet, von denen bis zum Jahresende 32 Bestand von derzeit 78.695 Medieneinheiten zu den Sammel- (2010: 2) vom Institut auch in Anspruch genommen worden schwerpunkten Molekularbiologie, Genetik, Zytologie, Taxono- sind. Im gleichen Zeitraum wurden drei neue Patentanmeldun- mie und Kulturpflanzenforschung. gen (2010: 1) durch das Institut vorgenommen. Durch die Beteiligung der Bibliothek am DFG-Projekt „Nutzung Das IPK verfügte damit zum Jahresende 2011 über 22 Betriebs- von Nationallizenzen“ konnte das Angebot an elektronischen geheimnisse (2010: 18) und war als alleiniger oder Mitanmelder Literaturressourcen deutlich verbessert werden. So kann nun- an 28 Patentfamilien (2010: 26) mit Anmeldungen in Deutsch- mehr jeder Wissenschaftler die Online-Archive von den Verla- land und im Ausland beteiligt. Daneben sind IPK-Erfinder an 28 gen Springer und Elsevier nutzen, d. h. ältere Zeitschriftenarti- Patentfamilien (2010: 22) beteiligt, die durch Industriepartner kel sind im Volltext zugänglich. Weiterhin wird für eine effektive im Rahmen von FuE-Verträgen angemeldet wurden. Literaturrecherche die Fachdatenbank „ISI-Web of Knowledge“ über ein Leibniz-Gemeinschaft-Konsortium erworben und be- In 2011 ging das Institut insgesamt 20 projektorientierte Ko- reitgestellt. operationsverträge (einschließlich von vier Unteraufträgen und einem Werkvertrag) (2010: 20, einschließlich von vier Un- Um der geänderten wissenschaftlichen Nachfrage Rechnung teraufträgen und zwei Werkverträgen) ein und schloss neun zu tragen, wurde in 2010 eine Bestands- und Bedarfsanalyse Forschungs- und Entwicklungsverträge (2010: 8 und 3 Lizenz- im Bereich der Zeitschriften durchgeführt. Als Ergebnis ist das verträge sowie zwei Verwertungsvereinbarungen) ab. Außer- IPK zum 1. Januar 2011 dem Leibniz-Gemeinschaft/Springer- dem wurden im Jahr 2011 63 Materialtransfer- und Geheim- Konsortium beigetreten. Damit verbunden ist der Erwerb des haltungsvereinbarungen (2010: 106) (exklusive der Abteilung Online-Paketes Lebenswissenschaften mit einem Umfang von Genbank) mit in- und ausländischen Forschungseinrichtungen ca. 500 Online-Zeitschriftentiteln. Bereits aktuell zu beobach- sowie Wirtschaftsunternehmen abgeschlossen. ten ist die vermehrte Nutzung des Onlineangebots verbunden Die Einnahmen aus der industriefinanzierten Auftragsfor- mit einem Rückgang an Literaturanforderungen im Leihver- schung betrugen im Jahr 2011 rund 2,4 Mio. Euro (2010 insge- kehr. Auch zukünftig sollen regelmäßige Analysen des Bedarfs samt 1,8 Mio. Euro). und Nutzerverhaltens sowohl die Effizienz steigern als auch das Serviceangebot der Wissenschaftlichen Bibliothek anpassen.

Raumprogramm und Baumaßnahmen/ Als öffentliche Bibliothek werden die Bestände auch über das Facilities and Constructions IPK hinaus überwiegend von den auf dem Campus Gatersleben Ausgehend von 14.714 m² Hauptnutzfläche (HNF) wurden dem ansässigen Biotechnologiefirmen sowie von Praktikanten des IPK aufgrund erhöhten Platz- und Raumbedarfs für seine Arbei- „Grünen Labors“ genutzt. Der Bibliotheksbestand ist in zahl- ten durch das Kultusministerium des Landes Sachsen-Anhalt reichen elektronischen Fachkatalogen, wie z. B. dem Gemein- weitere Flächen zur Nutzung bewilligt: samen Bibliotheksverbund (GBV), dem Periodikabestand in der Zeitschriftendatenbank (ZDB) etc. bibliographisch nachgewie- insgesamt 20.939 m² Hauptnutzfläche sen, womit die IPK-Bibliothek ihren Beitrag zur regionalen und davon 15.703 m² HNF in Labor- und Bürogebäuden und überregionalen Literaturversorgung leistet. 5.236 m² HNF in Gewächshäusern.

Dies war die Voraussetzung zum Bau des neuen Gewächshaus- komplexes am Genomzentrum, zum Umbau von Flächen zur Aufnahme zusätzlicher Phytokammernkapazität sowie zur Sa- nierung des Technischen Servicegebäudes und zusätzlichen Räumlichkeiten für die Lagerung von Erntegut.

20 Abb. 8 Entwicklung der Gesamteinnahmen Fig. 8 Development of total revenues

Abb. 9 Entwicklung der Gesamtausgaben Fig. 9 Development of total expenditure

Abb. 10 Entwicklung der Drittmitteleinnahmen nach Mittelherkunft ohne Anteil für Partner Fig. 10 Development of third party funds excl. partner shares (source of funds)

21 Abteilung Genbank/ Department of Genebank

Abb. 11 Fig. 11 Die Erstellung einer physikalischen Karte bildet die Grundlage für die karten- A physical map is the foundation for map-based sequencing of the barley gestützte Sequenzierung des Gerstengenoms. Das IPK (Ag Genomdiversität) genome. IPK (Genome Diversity group) is developing a physical map by fin- erarbeitet gegenwärtig eine physikalische Karte des Gerstengenoms, durch gerprinting analysis of BAC clones of a genomic DNA library of barley. Cleav- sogenannte Fingerprintkartierung von BAC-Klonen aus genomischen DNA- age by restriction enzymes is resulting in specific fragment patterns partially Bibliotheken. BAC-Klone werden mit Restriktionsenzymen geschnitten, so shared by overlapping BAC clones. This information can be utilised to form dass definierte Fragmentmuster entstehen. Diese erlauben es, physikalisch so-called contigs. A classical visualisation of a 4 Mbp large contig 44072 of überlappende Klone durch (zumindest teilweise) identische Fragmentmus- the barley physical map is shown in Fig. 11A. BAC clones labeled in dark have ter zu identifizieren. Aus überlappenden BAC-Klonen entstehen sogenannte been anchored to the genetic map of chromosome 3H. The new software Contigs. Abb. 11A zeigt eine klassische Darstellung des Contigs 44072, der eine LTC (Frenkel et al. 2010, BMC Bioinformatics 11:584) allows for an improved Länge von ca. 4 Mbp besitzt. Dunkel markierte BAC-Klone sind in der geneti- visualisation of the multidimensional relationships of BAC clones within schen Karte von Chromosom 3H verankert. Mit Hilfe der Software LTC (Frenkel contigs revealing the structure of a network (Fig. 11B-D). The analysis of the et al. 2010, BMC Bioinformatics 11:584) lassen sich die wesentlich komplexeren same contig 44072 by LTC indicated a completely linear topology of its entire Überlappungsbeziehungen der einzelnen BAC-Klone eines Contigs als Netz- length of 4Mbp (Fig. 11B). (R. Ariyadasa, Z. Frenkel, A. Korol, N. Stein) werk darstellen (Abb. 11B-D) und auf ihre lineare Topographie hin überprüfen. Contig 44072 besitzt über die gesamte Länge von 4 Mbp eine vollständig lineare Topologie (Abb. 11B). (R. Ariyadasa, Z. Frenkel, A. Korol, N. Stein)

22 Abteilung Genbank Department of Genebank Leiter: Prof. Dr. Andreas Graner Head: Prof. Andreas Graner

Allgemeine Forschungsziele General research goals Die Bundeszentrale Ex-situ-Genbank für landwirtschaftliche und The Federal ex situ Genebank for agricultural and horticultural gartenbauliche Kulturpflanzen erhält eine der weltweit größ- crops is one of the largest collections worldwide. It provides ten Sammlungen von pflanzengenetischen Ressourcen (PGR). essential contributions to the implementation of the National Sie liefert wesentliche Beiträge zur Umsetzung der Nationalen Strategy for Biological Diversity and to the National Programme Strategie zur Biologischen Vielfalt und des Nationalen Fachpro- for the Conservation and Sustainable Utilisation of Plant Ge- gramms zur Erhaltung und nachhaltigen Nutzung pflanzenge- netic Resources of Agricultural and Horticultural Crop Plants. netischer Ressourcen landwirtschaftlicher und gartenbaulicher At the European level, the Genebank is connected with the Kulturpflanzen.A uf europäischer Ebene ist die Genbank darüber European Cooperative Programme for Plant Genetic Resources hinaus auf vielfältige Weise mit dem European Cooperative Pro- (ECPGR) in manifold ways. The overriding task of the depart- gramme for Plant Genetic Resources (ECPGR) verbunden. Die zen- ment is the preservation and the characterisation of its genetic trale Aufgabe der Abteilung liegt in der Erhaltung der Sammlung resources as well as the development of strategies aiming at und Bereitstellung von Material. In diesem Zusammenhang wer- their improved utilisation. In this context, comprehensive serv- den umfangreiche Dienstleistungen wie die Bereitstellung von ice activities are performed such as the allocation of seed, tuber Saat- und Pflanzgut sowie damit verbundenen Informationen and plant samples along with the corresponding information. erbracht. Die in der Abteilung bearbeiteten Forschungsthemen Research topics of the department include the continued op- umfassen die fortgesetzte Optimierung des Erhaltungsmanage- timisation of the conservation management, investigation of ments, die Untersuchung von Artbildungs- und Anpassungspro- biological processes involved in speciation and adaptation, zessen, die Aufklärung taxonomischer Zuordnungen innerhalb clarification of taxonomic assignments within selected genera ausgewählter Kultur- und Wildpflanzengattungen sowie die of crop and wild species and molecular genetic analysis of ag- (molekular)genetische Analyse agronomischer Merkmale. ronomically relevant characters and traits.

Entwicklungen im Berichtszeitraum Developments in 2010 and 2011

Sammlungsmanagement Management of the ex situ collection Im Zentrum des Sammlungsmanagements steht der Betrieb der The Federal ex situ Genebank comprises 151,002 accessions Bundeszentralen Ex-situ-Genbank. Sie umfasst zum gegenwärti- from 3,212 species and 776 genera. Besides this living collec- gen Zeitpunkt 151.002 Akzessionen aus 3.212 Arten und 776 Gat- tion, the Genebank maintains a comprehensive herbarium tungen. Neben den Lebendsammlungen unterhält die Genbank which serves as reference centre for taxonomical studies and als Referenzzentrum für taxonomische Arbeiten ein Herbarium holds more than 420,000 voucher specimen as well as a col- mit über 420.000 Belegen sowie über 154.000 Referenzmustern lection representing 154,000 reference samples of spikes and von Getreideähren und Früchten. fruits. Die Erhaltung der Sammlung erfolgte in Gatersleben (130.620 The collection is maintained in Gatersleben (130,620 acces- Akzessionen) und den beiden Außenstandorten Groß Lüsewitz sions) and two satellite stations located at the Baltic Sea, Groß (Kartoffelsortiment, 6.124 Akzessionen) und Malchow (Öl- und Lüsewitz (potato assortment, 6,124 accessions) and Malchow Futterpflanzen, 14.258 Akzessionen). Etwa 3.200 Akzessionen (oil and forage crops, 14,258 accessions). About 3,200 acces- werden in vitro erhalten, hierunter befinden sich 2.900 Kartof- sions are preserved in vitro, including 2.900 potato accessions. felakzessionen. Zur Absicherung der Lebendsammlung wird die A cryo-collection of potato is continuously extended and cur- Cryo-Sammlung von Kartoffel kontinuierlich ausgebaut und um- rently comprises 1,289 accessions. It serves as a safety backup fasst gegenwärtig 1.289 Akzessionen. Parallel hierzu wurde mit of the living collection. During the reporting period, a cryo-col- dem Aufbau einer Cryo-Sammlung für Knoblauch (Allium sati- lection of garlic (Allium sativum) was initiated, presently com- vum) begonnen, die gegenwärtig 96 Akzessionen umfasst. prising 96 accessions. Etwa 4 % der Sammlung (6.658 Akzessionen) werden vegetativ About 4 % of the collection (6,658 accessions) is propagated erhalten, der Rest wird über Samen vermehrt. Der Vermehrungs- vegetatively, the rest is maintained as seeds. In the growing anbau von 7.894 Akzessionen entspricht ca. 6 % der Samenträger, season 2010/2011 7,894 accessions were grown for seed mul- woraus sich eine mittlere Einlagerungszeit von etwa 17 Jahren ab- tiplication, corresponding to about 6 % of the total collection, leiten lässt. resulting in an average storage time per accession of about 17 Zur Absicherung der weltweit in Genbanken gelagerten Saat- years. gutbestände eröffnete der Global Crop Diversity Trust im Jahr In order to increase the safety of the 7.4 million-odd seed sam- 2008 im Permafrostboden von Spitzbergen ein Langzeitlager. ples maintained in genebanks across the world, the Global

23 Abteilung Genbank/Department of Genebank

Das IPK nutzt diese Möglichkeit zur Einlagerung von Sicherheits- Crop Diversity Trust opened up a long-term storage facility duplikaten und hat seit der Eröffnung des Global Seed Vault established under permafrost conditions on the Norwegian nahezu 30.000 Samenmuster nach Spitzbergen geschickt. Die island of Svalbard in 2008. IPK followed the invitation to store Genbank wird diese Aktivität fortsetzen, bis Sicherheitsduplikate safety duplicates in the Global Seed Vault and has transferred aller Samenträger in den Kühllagern auf Spitzbergen deponiert by now some 30.000 seed samples. The Genebank will continue wurden. this effort in the years to come, until the whole collection will In den beiden vergangenen Jahren wurden insgesamt 21.788 be secured in this way. bzw. 33.878 Muster abgegeben. Dies bedeutet einen erhebli- In the past two years, 21,788 and 33,878 samples were dis- chen Anstieg gegenüber den Vorjahren. Die durchschnittliche tributed, respectively. In comparison to previous years this Bearbeitungsdauer für Bereitstellung und Versand des Materials represents a substantial increase. The average handling time betrug 12 Tage ab dem Eingang der unterzeichneten Standard- amounts to 12 days from receipt of the signed Standard Ma- Materialtransfer-Vereinbarung (SMTA). Sortimentsumfänge und terial Transfer Agreement (SMTA) until dispatch of the seeds/ Abgaben sind Tabelle 1, S. 25 zu entnehmen. tubers/plants. Further information on the collection and the Im Berichtszeitraum stellten Privatpersonen mit insgesamt distribution of accessions are presented in Table 1, p. 25. 23.260 Akzessionen die größte Nutzergruppe von Genbankma- In the reporting period, private individuals receiving a total of terial dar, gefolgt von Forschungseinrichtungen (18.173) und 23,260 accessions represented the largest user group, followed Pflanzenzüchtern (7.901). Rund 23.000 Muster (41 %) wurden an by research institutes (18,173) and plant breeders (7,901). Nutzer im Ausland abgegeben. About 23,000 accessions (41 %) were shipped abroad. Seit 2007 verfügt die Genbank über ein Qualitätsmanagement- Since 2007 the Genebank deploys a Quality Management system nach DIN EN ISO 9001:2008. Darin sind sämtliche Kern- System (QM) according to standard DIN EN ISO 9001:2008. It prozesse des Erhaltungsmanagements dokumentiert und durch documents and surveys all core processes pertaining to the Verfahrensregelungen und Arbeitsanweisungen unterlegt. Das conservation management. The QM warrants transparency and Qualitätsmanagementsystem sichert Transparenz und Nachvoll- traceability of all relevant steps and provides the basis for the ziehbarkeit der in der Genbank durchgeführten Arbeiten und sustainable preservation of the ample know-how required for liefert damit die Grundlage für die nachhaltige Sicherung des für the conservation management. In 2010 the certificate was re- die Sortimentserhaltung notwendigen Know-how. Zur Überprü- confirmed by an external audit. fung des Qualitätsmanagementsystems sowie zur kontinuierli- chen Verbesserung der Arbeitsprozesse werden in regelmäßigen Research Abständen interne Audits durchgeführt. Im Frühjahr 2010 wurde Research within the Genebank is structured into three coher- im Ergebnis eines externen Wiederholungsaudits der Zertifizie- ent programmes: “Management and Evaluation”, “Characterisa- rungsstatus um weitere drei Jahre verlängert. tion and Documentation” as well as “Taxonomy and Evolution”. At first approximation the corresponding activities can be as- Forschung signed on the one hand to research that is related to the collec- Die Abteilung Genbank ist in drei thematisch weitgehend ko- tion proper and on the other hand to enhance the utilisation of härente Forschungsbereiche gegliedert: „Charakterisierung plant genetic resources. The former aim at improvement of the und Dokumentation“, „Management und Evaluierung“ sowie conservation management of the collection whereas the latter „Taxonomie und Evolution“. In erster Annäherung lassen sich die aim at valorisation of plant genetic resources for the genetic Forschungsarbeiten in sammlungsbezogene und nutzungsbe- improvement of crop plants. Major emphasis is put on imple- zogene Themen differenzieren. Das Ziel der sammlungsbezo- mentation of molecular genetic approaches for cereal species, genen Forschung ist ein verbessertes Erhaltungsmanagement. for trait mapping and gene discovery as well as research into Die nutzungsbezogenen Forschungsarbeiten konzentrieren evolution and domestication. In addition, research into taxon- sich auf die Entwicklung von Ressourcen und Konzepten zur omy and evolution addresses mainly fundamental questions verbesserten Inwertsetzung der Gaterslebener Sammlung für regarding speciation, adaptation and radiation of crop plants die züchterische Anpassung von Kulturpflanzen. Hierbei stehen and their wild relatives. molekulargenetische Ansätze bei Getreide im Vordergrund. Eine The programme “Taxonomy and Evolution” deals with ques- Mittelstellung zwischen den beiden genannten Themen neh- tions regarding the evolution of crop plants and related spe- men die Forschungsarbeiten zur Kulturpflanzentaxonomie und cies (Hordeum, Allium, Hypericum etc.). The genus Hordeum has -evolution ein. Dort werden in erster Linie Fragestellungen aus been used as a model for temperate grasses to gain insight dem Bereich der Gundlagenforschung bearbeitet. into phylogenetic relationships, ecology and demographic Im Zentrum des Forschungsbereichs „Taxonomie und Evoluti- history, because of the large significance of cultivated barley on“ stehen die Untersuchung von Artbildungsprozessen und die (Hordeum vulgare) both for agriculture and for research across Aufklärung taxonomischer Beziehungen innerhalb ausgewählter all departments of the Institute. This genus, comprising 31 spe- Gattungen (Hordeum, Allium, Hypericum, u. a.). Hierbei ist die Gat- cies, is characterised by an extraordinary diversity. It comprises tung Hordeum aufgrund der großen Bedeutung der Kulturgerste both inbreeding and outbreeding species, diploid, tetraploid (Hordeum vulgare) in der landwirtschaftlichen Praxis und für die and hexaploid species, as well as annual and perennial forms. Forschungsarbeiten am Institut von besonderem Interesse. Da- By means of phylogenetic and population genetic approaches neben zeichnet sich die 31 Arten umfassende Gattung durch eine as well as niche modelling, fundamental knowledge has been

24 Tabelle/Table 1: Übersichten zum Bestand und zu den Transfers der Genbank nach Fruchtarten/Overview of collection and transfers from Genebank by crop species

Bestand/ Abgaben/Distribution Sortimente Assortments Accessions 2010 2011

Getreide und Gräser Cereals and Grasses 65448 6725 7036 Weizen wheat 28111 3330 2932 Gerste barley 23245 1571 1031 Hafer oat 4835 315 119 Roggen rye 2411 147 273 Triticale Triticale 1581 8 26 Aegilops Aegilops 1531 676 1549 Hirsen millets 845 217 474 Mais maize 1550 298 245 Gräser grasses 1339 163 387 Leguminosen Legumes 28066 3470 3163 Phaseolus Phaseolus 9146 1421 776 Ackerbohnen field beans 3283 399 344 Sojabohnen soybeans 1514 176 70 Bohnen-Sonderkulturen other beans 624 444 312 Erbsen pea 5295 247 119 Kichererbsen chickpea 531 80 799 Lathyrus vetchling 524 30 64 Wicken vetches 1878 223 383 Lupinen lupins 2769 103 75 Linsen lentils 475 113 101 Kleearten clover species 1739 139 81 Sonstige others 288 95 39 Cucurbitaceae Cucurbitaceae 2671 691 1311 Kürbisse pumpkins 1081 291 597 Melonen melons 726 203 196 Gurken cucumbers 709 126 459 Sonstige others 155 71 59 Gemüse (+ Rüben) Vegetables 18794 3618 10838 Tomaten tomatoes 3544 680 2969 Paprika pepper 1532 596 407 Eierfrüchte eggplants 112 32 70 Beta Beta 2320 254 100 Raphanus Raphanus 750 178 89 Möhren carrots 500 141 2804 Zichorie chicory 690 98 164 Allium Allium 3319 275 470 Brassica Brassica 2184 411 262 Salat lettuce 1130 146 399 Spinat spinach 214 152 91 Sellerie celery 252 50 508 Quinoa Quinoa 957 11 1237 Sonstige others 1290 594 1268 Öl-, Faser-, Farbpflanzen Oil, Fibre, Dye Plants 5526 1545 1844 Lein flax 2324 347 92 Sonnenblumen sunflower 691 405 256 Farbpflanzen dye plants 481 254 291 Faserpflanzen fibre plants 187 65 185 Ölpflanzen oil plants 557 234 488 Sonstige others 1286 240 532 Arznei-, Gewürzpflanzen Medicinal, Spice Plants 8344 2370 5273 Mohn poppy 1149 141 239 Tabak tobacco 590 61 145 Sonstige others 6605 2168 4889 Mutanten Mutants 1771 51 108 Tomaten tomatoes 744 35 79 Soja soybean 567 2 9 Antirrhinum Antirrhinum 460 14 20 Kartoffeln Potatoes 6124 1981 1965 Öl- und Futterpflanzen Oil and Forage Crops 14258 1327 2340 Raps und Futterkohl rapeseed and feeding kale 2472 714 1847 Gräser grasses 10441 584 446 Rotklee und Luzerne red clover and alfalfa 1345 29 47 Gesamt/Total 151002 21778 33878

25 Abteilung Genbank/Department of Genebank

außerordentliche Vielfalt aus. In ihr finden sich sowohl Selbst- als gained regarding spatio-temporal processes of speciation and auch Fremdbefruchter, diploide, tetraploide und hexaploide radiation within this species. Moreover, it could be shown that Arten sowie einjährige und perennierende Formen. Durch den the emergence of polyploid species mainly results from inter- Einsatz phylogenetischer und populationsgenetischer Ansätze specific hybridisation events, as all but one of the polyploid spe- sowie mit Hilfe der Nischenmodellierung konnten in den vergan- cies are alloploids. In the context of elaborating a comprehen- genen Jahren grundlegende Erkenntnisse über den räumlichen sive taxonomy for the genus Allium, which is likewise coined by und zeitlichen Verlauf von Artbildung und Ausbreitung innerhalb an extraordinary amount of diversity, several new species were der Gattung gewonnen werden. Darüber hinaus wurde gezeigt, collected and described in the context of research in Central dass die Entstehung polyploider Hordeum-Arten in der Mehrzahl Asia, which represents the natural range for many species in auf die Hybridisierung diploider, teilweise bereits ausgestorbener this genus. Furthermore substantial progress was made regard- Arten zurückzuführen ist. Nur bei einer Art konnte das Auftreten ing the elucidation of the taxonomic structure with the subge- autopolyploider Formen nachgewiesen werden. Bei der Erarbei- nus Melanocrommyum. tung einer umfassenden Taxonomie für die ebenfalls durch große Research within the programme “Management and Evalu- Vielfalt gekennzeichnete Gattung Allium konnten im Zuge von ation” focuses on further optimisation of the conservation Sammelreisen in die natürlichen Verbreitungsgebiete in Zen- management. In this context, the maximum storage duration tralasien eine Reihe neuer Arten identifiziert werden. Ergänzend for seeds is an important parameter regarding the economy hierzu wurden wesentliche Fortschritte bei der Aufklärung der and efficiency of the conservation management.A s mentioned taxonomischen Beziehungen innerhalb der Untergattung Mela- above, seed borne accessions make up for the vast majority of nocrommyum erzielt. the collection and each increase in the average seed longev- Im Mittelpunkt der Forschungsarbeiten im Bereich „Manage- ity would represent an important contribution to the improvement und Evaluierung“ steht die weitere Verbesserung des ment of the efficiency of the conservation management.H ence, Erhaltungsmanagements von vegetativ bzw. von über Samen genetic and physiological processes are investigated that lead vermehrten Genbankmustern. Im Hinblick auf die Erhaltung ve- to a decrease in the germination ability of seeds. Elucidation of getativ vermehrter Pflanzenarten wurde an der weiterenE tablie- these processes may provide important clues regarding the im- rung und Optimierung der Protokolle für Cryo-Konservierung provement of seed longevity. In this regard, genetic analyses in von meristematischem Pflanzengewebe gearbeitet. Allerdings wheat and barley resulted in identifying first TQ Ls for this trait. stellen samenvermehrte Akzessionen den weitaus größten An- Moreover, it could be demonstrated that the ratio of oxidised to teil der Sammlung dar. Dementsprechend ist die Lagerdauer von reduced glutathion is a useful indicator of seed longevity. Saatgutproben ein wichtiger Faktor für die Effizienz des Erhal- The programme “Characterisation and Documentation” tungsmanagements. Einen weiteren Forschungsschwerpunkt comprises the characterisation of genetic resources at the DNA in diesem Bereich stellt daher die Aufklärung der Prozesse, die level as well as the development and curation of databases for zum Abbau der Keimfähigkeit von Saatgut führen, dar. Hieraus storage and provision of genebank-related data. In this regard, werden längerfristig wichtige Hinweise zur Verbesserung der the Genebank Information System (GBIS) acts as a central hub Langlebigkeit von Saatgut erwartet. In diesem Zusammenhang for both storage and provision of accession-based data. The wurden im Rahmen genetischer Untersuchungen bei Gerste system has implemented all steps of the conservation man- und Weizen erste QTL für dieses Merkmal identifiziert. Des Wei- agement and purchase order processing. It is continuously teren konnte gezeigt werden, dass das Verhältnis von oxidiertem updated regarding the implementation of new functionalities, zu reduziertem Glutathion einen geeigneten Indikator für die such as a module for the administration of characterisation and Lebensfähigkeit von Gerstensaatgut darstellt. evaluation data. In addition the system serves as an interface Der Forschungsbereich „Charakterisierung und Dokumentati- for the export of data into national and international databases on“ befasst sich mit der molekulargenetischen Charakterisierung on plant genetic resources. von Genbankmaterial auf DNA-Ebene sowie mit der Entwicklung To further improve the valorisation of plant genetic resources, und Pflege von Datenbanken zur Speicherung und Bereitstel- information is required on position, structure and function of lung von genbankbezogenen Daten. Ein zentrales Element für genes involved in the expression of agricultural characters and die Speicherung und Verwaltung akzessionsbezogener Informa- traits. In this context, research into trait mapping and gene iso- tionen stellt das Genbankinformationssystem (GBIS) dar. In dem lation is mainly focused on barley and wheat. Genetic mapping System sind sämtliche Prozesse des Erhaltungsmanagements increasingly targets quantitative traits and trait components by und des Bestellwesens abgebildet. Es erfolgt eine kontinuierliche employing genome-wide linkage disequilibrium mapping us- Weiterentwicklung im Hinblick auf die Implementierung neuer ing SNP marker chips for high-throughput genotyping, which Funktionalitäten, wie z. B. einem Modul für die Verwaltung von have been developed together with partners from the public Charakterisierungs- und Evaluierungsdaten. Das System stellt and the private sector. Gene isolation is still focused on mono- zudem die Schnittstelle für den Datenexport zur Aktualisierung genic traits such as spike morphology (row-type, branching, nationaler und internationaler Datenbanken zu pflanzengeneti- compactness) and virus resistance. Research activities focus- schen Ressourcen dar. ing on the development of a genomic sequence of barley have Wichtige Voraussetzung für die verbesserte Nutzbarmachung been initiated back in 2006 in the frame of the International der in den Sammlungen vorhandenen genetischen Diversi- Barley Sequencing Consortium (IBSC). Even when employing tät sind Informationen zur Position, Struktur und Funktion von the most advanced sequencing technologies only small runs

26 Genen, welche an der Ausprägung agronomischer Merkmale of DNA can be deciphered (although by the billions). The as- beteiligt sind. Die in diesem Zusammenhang in der Abteilung sembly of such sequence fragments is strongly compromised durchgeführten Forschungsarbeiten zur Merkmalskartierung by repetitive DNA, which is highly abundant in cereal gen- und Genklonierung konzentrieren sich auf Gerste und Weizen. omes. The availability of a physical map represents an important Bei den Kartierungsarbeiten steht in zunehmendem Umfang die prerequisite to reduce the complexity of the DNA fragments to Erfassung quantitativer Merkmalsvariation mit Hilfe der genom- be sequenced in order to facilitate assembly of a coherent ge- weiten Assoziationskartierung im Vordergrund. Hierbei werden nomic sequence. Towards the construction of a physical map SNP-Marker-Chips für die Hochdurchsatz-Genotypisierung ein- of the barley genome the seven barley chromosomes were re- gesetzt, an deren Entwicklung das Institut beteiligt war. Wei- constituted by a scaffold of overlapping DNA fragments cloned terführende Arbeiten zur Genklonierung konzentrieren sich auf into bacterial plasmid vectors (BAC clones, Fig. 11, p. 22). This monogen vererbte Merkmale, wie Ährenmorphologie (Zeiligkeit, allows for sequencing and assembly of defined chromosomal Verzweigung, Kompaktheit) und Virusresistenzen. regions by selecting a corresponding set of overlapping BACs Eine wesentliche Beschleunigung und Erleichterung der zeit- und forming a so-called minimum tiling path. Even though there ressourcenaufwändigen kartengestützten Klonierung agrono- are still obstacles to be negotiated on the way to a high quality misch relevanter Gene stellt die Verfügbarkeit einer genomischen genomic sequence of barley, the presently available sequence Sequenz dar. Technologische Fortschritte auf den Gebieten der information comprises nearly all barley genes and yielded DNA-Fragmentanalyse und -sequenzierung sowie in der Bioinfor- important findings about the evolution of this genome. The matik haben die Entschlüsselung komplexer Nutzpflanzengeno- sequence information also facilitated the development of me in den Bereich des Machbaren gerückt. Entsprechende Arbei- manifold analytical resources such as SNP markers and DNA ten zur Sequenzierung des Genoms der Kulturgerste (Hordeum arrays. In the foreseeable future these will usher in the system- vulgare) wurden bereits im Jahr 2006 im Rahmen eines durch das atic re-sequencing of genebank collections and thereby blaze Institut ins Leben gerufenen internationalen Konsortiums (IBSC) the trail for a thorough investigation and a knowledge-based aufgenommen. Da sich jedoch auch mit modernsten Sequenzier- valorisation of plant genetic resources. Further details of the technologien immer nur kleine DNA-Abschnitte entschlüsseln mentioned and many other research and service activities of lassen, deren Assemblierung durch hochredundante, repetitive the department will be found in the subsequent reports of the DNA-Fragmente häufig verhindert wird, stellt dieE ntwicklung ei- individual research groups. ner physischen Karte eine wichtige Voraussetzung für die Erstel- lung einer kohärenten genomischen Sequenz dar. Bei dem Auf- bau einer solchen Karte wurden die sieben Gerstenchromosomen Andreas Graner, November 2011 durch ein Gerüst überlappender, in bakterielle Plasmidvektoren klonierter DNA-Fragmente (BAC-Klone) rekonstituiert (Abb. 11, S. 22). Einzelne Chromosomenbereiche können nun durch Auswahl entsprechender, überlappender BAC-Klone systematisch sequen- ziert und assembliert werden. Auch wenn auf dem Weg zu einer qualitativ hochwertigen Genomsequenz noch einige Hindernisse überwunden werden müssen, lieferten die bereits verfügbaren Sequenzen, in denen nahezu alle Gene der Gerste erfasst sind, wichtige Erkenntnisse zur Evolution dieses Nutzpflanzengenoms. Sie ermöglichten darüber hinaus die Entwicklung einer Vielfalt analytischer Ressourcen für die Genomanalyse. Diese werden in absehbarer Zeit die systematische Resequenzierung umfangrei- cher Genbankkollektionen einleiten und damit neue Wege für die weitere Erforschung und die wissensbasierte Inwertsetzung pflanzengenetischer Ressourcen eröffnen. Weitere Einzelheiten zu den aufgeführten Forschungsthemen und damit verbundenen Projekten sind den nachfolgenden Be- richten der einzelnen Arbeitsgruppen zu entnehmen.

Andreas Graner, November 2011

27 Abteilung Genbank/Department of Genebank

Programme: Characterisation and Documentation Research Group: Genome Diversity Head: Prof. Andreas Graner, Dr. Nils Stein

Scientists Shaaf, Salar (Iranian government, 11.02.-25.09.2010) Shi, Bu-Jun, Dr. (University of Adelaide, 27.02.-02.03.2011) IPK financed Turktas, Minne, Dr. (self-financed, 22.03.-26.03.2010) Ariyadasa, Ruvini Tharanga, Dr. (Pakt für Forschung und Yang, Ping (self-financed, 24.08.-31.08.2010) Innovation, since 01.07.2011) Himmelbach, Axel, Dr. (Pakt für Forschung und Innovation, 01.05.2010-31.10.2011) Goals Kilian, Benjamin, Dr. (till 28.02.2014) Pasam, Raj Kishore (0.50, since 01.07.2011) Development of genomics-based approaches to valorise plant Poursarebani, Naser (0.50 Pakt für Forschung und Innovation, genetic resources of barley (Hordeum vulgare) to facilitate the 01.07.-31.12.2011) understanding of structural and functional principles that Sharma, Rajiv (0.50, since 30.06.2011) impinge on variation in agronomic and adaptive traits as well as identification of the effects of evolutionary and selective Grant Positions forces that shape the genome of this species. Ariyadasa, Ruvini Tharanga, Dr. (BMBF, till 30.06.2011) Dhanagond, Sidram (0.50 BMBF, since 17.11.2011) Himmelbach, Axel, Dr. (BMBF, since 01.11.2011) Research Report Jost, Matthias (0.50 DFG, 01.07.-31.10.2011; 0.50 BMBF, since 01.11.2011) Knowledge about the structure and function of genes that Neumann, Kerstin (0.50/1.00 BMBF, since 01.09.2010) underlie agronomic traits forms a prerequisite for the systematic Pasam, Raj Kishore (0.50 BMBF, till 30.06.2011) analysis and exploitation of the genetic resources of a given Poursarebani, Naser (0.50 BMBF, till 30.06.2011) crop plant. Against this backdrop the research programme Saal, Bernhard, Dr. (BMBF, 01.01.-30.04.2010) connects phenotypic analysis, trait mapping and structural Schmutzer, Thomas (0.50 BMBF, till 31.12.2010) genomics to conduct both hypothesis-driven and explorative Sharma, Rajiv (0.50 DFG, till 29.06.2011) research aiming at the identification and validation of genes Yang, Ping (0.50 BMBF, since 01.09.2010) with agricultural relevance. Genomics resources are being Zhou, Ruonan, Dr. (0.50 BMBF, till 30.06.2011; 0,50 EU) developed with special focus on barley (Hordeum vulgare) and comprise high-density genetic marker maps, a physical BAC- Visiting Scientists/Scholars contig map and a genomic sequence. Activities with regard Amatriain, Maria Munoz, Dr. (University of Minnesota, to trait mapping include the analysis of biparental progenies 06.06.-17.07.2011) to develop high-resolution maps for selected characters and Athmer, Benedikt (self-financed, 01.04.2010-31.12.2011) the analysis of quantitative traits based on whole genome Bartos, Jan, Dr. (self-financed, 22.03.-26.03.2010) association mapping. To facilitate an in-depth analysis of Filatenko, Anna, Dr. (DFG, 22.05.-31.10.2011) quantitative traits, a phenotyping project has been started to Fricano, Agostino (BMBF, 22.03.-26.03.2010) analyse plant growth over time using automated image capture Gottwald, Sven, Dr. (self-financed, till 28.02.2011) and image analysis systems. Gradin, Therese (Södertörn University, 01.02.-28.02.2010) Imri, Ben-Israel (IPK and self-financed, 19.06.-26.06.2011) In order to characterise the extent of linkage disequilibrium (LD) Konovalov, Fedor A., Dr. (DFG, 01.03.-31.05.2010; self-financed, 1,536 SNPs were interrogated to genotype a comprehensive 18.11.-18.12.2010; IPK, 04.06.-19.06.2011) panel of accessions representing three different gene pools: Kurowska, Marzena, Dr. (IAEA fellowship, University of Silesia, (i) 282 European spring and 112 European winter barley 29.10.2011-31.01.2012) cultivars (HVCC), (ii) 322 landrace accessions from Syria and Maccaferri, Marco, Dr. (University Bologna, 22.03.-26.03.2010) Jordan (LRC) and (iii) 216 wild barley (Hordeum vulgare ssp. Manninen, Outi, Dr. (self-financed, 22.03.-26.03.2010) spontaneum) accessions from Israel (HSC). Genome-wide LD Moisy, Cedric, Dr. (self-financed, 22.03.-26.03.2010) decay was found to be within the range of 7-10 cM in HVCC, <5 Obidiegwo, Oskar (University of Hohenheim and IPK, cM in LRC and <1 cM in HSC. In collaboration with the research 28.08.-02.09.2011) group Transcriptome Analysis all gene pools were screened for Paquariello, Marianna, Dr. (self-financed, 15.03.-30.05.2010 and quantitative resistance to powdery mildew (Blumeria graminis 28.08.-27.09.2011) f.sp. hordei) using poly-virulent pathotypes. Moreover, twenty Perovic, Dragan, Dr. (JKI Quedlinburg, 01.01.-30.04.2010 and agronomical traits were scored in eight European environments 01.06.-31.12.2010) from 2008 till 2010 in collaboration with an EU-wide consortium. Schulte, Daniela, Dr. (self-financed, 01.02.-31.12.2010) Using genome-wide association (GWA) analysis significant

28 SNP associations were found for a series of agronomic characters SSR markers to assess the genetic diversity and population and traits including plant height, heading date and internode structure. A total of 372 alleles were detected among which 152 length (R. Sharma, A. Graner, B. Kilian). In a similar approach, a are rare alleles. Given the genetic and geographic diversity, this panel of 224 spring barley accessions was analysed based on landrace collection forms a resource for future association 918 informative SNPs and 1,037 DArT markers 57 novel QTL studies (R. Pasam, A. Graner, B. Kilian). were detected (R. Pasam, B. Kilian, A. Graner). Being a member of the network of excellence in agricultural and The two studies revealed that despite the high extent of LD in nutrition research funded by the BMBF, non-invasive sensor cultivated barley the insufficient redundancy of SNP markers technologies were applied for systematic phenotyping. severely limits the genetic power of GWA mapping. Therefore, a Using a LemnaTec HTS-Scanalyzer 3-D system, plant growth high-density SNP chip (iSELECT, Illumina) has been developed has been monitored under various stress conditions via in a collaborative effort with the James Hutton Institute, automated image analysis. Plants were phenotyped on a daily Scotland and Trait Genetics GmbH. The array contains 7,864 basis using top and side cameras to capture images at different bi-allelic SNPs that were previously validated in a broad range wavelengths. Phenotypic data were analysed in collaboration of barley accessions to maximise marker information content with the research group Image Analysis. Correlations of and to minimise ascertainment bias. Fig. 12 shows the massive fresh weight and digital biomass were sufficient 2(R >0.95) to increase in significant effects, when increasing the number of estimate the accumulation of above ground biomass by using SNP markers from 918 to 5,600 (R. Pasam, B. Kilian). image data (K. Neumann, A. Graner, B. Kilian). Landraces are unexplored resources of allelic diversity and contain useful alleles for crop improvement. In order to capture The construction of a generic physical map is a central task the genetic and phenotypic diversity, a large collection of spring of the internationally coordinated effort towards sequencing barley landraces has been selected from the genebank. This set the whole barley genome (International Barley Sequencing of 1,491 purified landraces originating from 40 countries from Consortium, IBSC, http://barleygenome.org). To this end, an 5 º N to 62.5 º N and 16 º W to 71 º E, was genotyped with 45 automated fingerprint assembly was generated based on about

Fig. 12 Effects of marker density on genome-wide association analysis of heading date in a panel of 224 spring barley accessions.A ssociations were computed using a Mixed Linear Model using the kinship coefficient (K) to account for population structure. The K-matrix was based on 918 informative SNP markers. In the upper panel (a) the same 918 markers were employed for association analysis while in the lower panel (b) 5,600 SNP markers were analysed. The y-axis gives the significance level for each marker and the dashed line indicates the significance threshold of log P = 10-3. SNP markers are represented as dots with different colors representing the individual barley chromosomes (1H-7H). Markers are ordered according to their position on the genetic map, with the short arm of each barley chromosome pointing to the left. Approximate genetic positions of known genes for flowering time are indicated by arrowheads. Higher significance levels were attained in b) due to increased number of SNP markers (i) located in close vicinity to and (ii) in linkage disequilibrium with the marked flowering time genes (R. Pasam).

29 Abteilung Genbank/Department of Genebank

13-fold haploid genome coverage of BAC clones originating did not require any attempt of chromosome walking, since from 5 different BAC libraries of the barley cultivar ‘Morex’. the physical map provided direct access to a physical BAC This resulted in less than 10,000 BAC contigs representing 99 contig comprising the closest flanking markers on either side % of the barley genome. Anchoring of the draft contig map of the gene. While functional characterisation of the gene is in to the genetic map of barley could build on diverse datasets progress, high-resolution mapping of three additional recessive of marker/gene information obtained by different partners resistance loci is underway (P. Yang, A. Graner, N. Stein). of the IBSC consortium (IPK/Gatersleben, Helmholtz Center/ Munich, Leibniz Institute of Age Research/Jena, James Hutton Publications Institute/Dundee, University of California/Riverside, Kansas State University/Manhattan). This included on the one hand Peer Reviewed Papers genes/markers assigned to BAC addresses by PCR/array-based screening of multidimensional BAC pools. On the other hand, 2010 genetic marker information was integrated by in silico sequence Co c k r a m , J., J. Wh i t e , D.L. Zu l u a g a , D. Sm i t h , J. Co m a d r a n , M. comparison between BAC end sequences, sequenced BAC Ma c a u l a y , Z. Lu o , M.J. Ke a r s e y , P. We r n e r , D. Ha r r a p , C. Ta p s e l l , H. clones, Whole-Genome Shotgun Assembly sequence and Liu, P.E. He d l e y , N. St e i n , D. Sc h u l t e , B. St e u e r n a g e l , D.F. Ma r s h a l l , Genotyping-by-Sequencing signatures (D. Schulte, R. Ariyadasa, W.T. Th o m a s , L. Ra m s a y , I. Ma c k a y , D.J. Ba l d i n g , T.A. Co n s o r t i u m , R. R. Zhou, N. Poursarebani, N. Stein). Wa u g h & D.M. O’Su l l i v a n : Genome-wide association mapping to candidate polymorphism resolution in the unsequenced A Minimum Tiling Path (MTP) of the physical map comprising barley genome. Proc. Natl. Acad. Sci. U.S.A. 107 (2010) 21611- 70,000 BACs was defined as a major deliverable during physical 21616. map construction. The MTP was rearrayed in a chromosome- Dr u k a , A., J. Fr a n c k o w i a k , U. Lu n d q v i s t , N. Bo n a r , J. Al e x a n d e r , J. Gu z y - wise manner at CNRGV Toulouse, France, and is readily available Wr o b e l s k a , L. Ra m s a y , I. Dr u k a , I. Gr a n t , M. Ma c a u l a y , V. Ve n d r a m i n , for being utilised in any attempt of map-based sequencing F. Sh a h i n n i a , S. Ra d o v i c , K. Ho u s t o n , D. Ha r r a p , L. Ca r d l e , D. the barley genome. A project funded by the Leibniz “Pakt für Ma r s h a l l , M. Mo r g a n t e , N. St e i n & R. Wa u g h : Exploiting induced Forschung und Innovation” is taking advantage of this new variation to dissect quantitative traits in barley. Biochem. resource while heading for sequencing barley chromosome Soc. Trans. 38 (2010) 683-688. 3H. Based on previous experience of shotgun sequencing Gl a s z m a n n , J., B. Kilian, H. Up a d h y a y a & R. Va r s h n e y : Accessing BAC clones on the Roche/454 GSFLX Titanium platform, more genetic diversity for crop improvement. Curr. Opin. Plant than 3,000 BAC clones (40 %) of the chromosome 3H Minimum Biol. 13 (2010) 167-173. Tiling Path will be sequenced and assembled to Phase I status Ha s e n e y e r , G., S. St r a c k e , C. Pa u l , C. Ei n f e l d t , A. Br o d a , H.P. Pi e p h o , (A. Himmelbach, N. Stein). A. Gr a n e r & H.H. Ge i g e r : Population structure and phenotypic variation of a spring barley world collection set up for Next Generation Sequencing (Roche/454) of sorted barley association studies. Plant Breed. 129 (2010) 271-279. chromosomes allowed gathering information of the overall Ha s e n e y e r , G., S. St r a c k e , H.P. Pi e p h o , S. Sa u e r , H.H. Ge i g e r & A. Gr a n e r : gene content and gene-order of barley chromosome 1H in a DNA polymorphisms and haplotype patterns of transcription first pilot study. Subsequently, all further 12 barley chromosome factors involved in barley endosperm development are arms were shotgun sequenced to ~1-fold coverage and the associated with key agronomic traits. BMC Plant Biol. 10 resulting sequence information was used in frame of a close (2010) 5. collaboration with the Helmholtz Center/Munich, the James Ko c s y , G., B. At h m e r , D. Pe r o v i c , A. Hi m m e l b a c h , A. Sz ü c s , I. Va s h e g y i , P. Hutton Institute/Dundee and the Institute of Experimental Sc h w e i z e r , G. Ga l i b a & N. St e i n : Regulation of gene expression Botany/Olomouc for developing linear gene order maps by chromosome 5A during cold hardening in wheat. Mol. (“genome-zippers”) of the entire barley genome. The Genet. Genomics 283 (2010) 351-363. same approach was used to sequence the homoeologous Ma, L., G.T. Vu, V. Sc h u b e r t , K. Wa t a n a b e , N. St e i n , A. Ho u b e n & I. wheat chromosomes 1A, 1B and 1D and is further explored for Sc h u b e r t : Synteny between Brachypodium distachyon and sequencing all rye chromosomes (N. Stein). Hordeum vulgare as revealed by FISH. Chromosome Res. 18 (2010) 841-850. Besides the efforts of unlocking the barley genome for whole Na i r , S.K., N. Wa n g , Y. Tu r u s p e k o v , M. Pourkheirandish , S. Sinsuwongwat , genome sequencing, the genomic resources developed G.X. Ch e n , M. Sa m e r i , A. Ta g i r i , I. Ho n d a , Y. Wa t a n a b e , H. Ka n a m o r i , for barley are being used for map-based cloning of genes T. Wi c k e r , N. St e i n , Y. Na g a m u r a , T. Ma t s u m o t o & T. Ko m a t s u d a : underlying important economical or developmental/ Cleistogamous flowering in barley arises from the morphological traits. Emphasis is put on the elucidation of suppression of microRNA-guided HvAP2 mRNA cleavage. the genetic basis of Bymovirus resistance in barley. After the Proc. Natl. Acad. Sci. U.S.A. 107 (2010) 490-495. successful cloning of the gene rym4/5 resistance gene in 2005, Öz e r , S., T. Ka r a k ö y , F. To k l u , F.S. Ba l o c h , B. Kilian & H. Öz k a n : we made, in collaboration with the Julius Kühn-Institute/ Nutritional and physicochemical variation in Turkish kabuli Quedlinburg, substantial progress in the map-based isolation chickpea (Cicer arietinum L.) landraces. Euphytica 175 (2010) of a second recessive resistance gene. The value of the available 237-249. genomics resources is highlighted by the fact that this project

30 Öz k a n , H., M. Tu n a , B. Kilian, F.S. Ba l o c h , N. Mo r i & S. Oh t a : Genome Ha s e n e y e r , G., T. Sc h m u t z e r , M. Se i d e l , R. Zh o u , M. Ma s c h e r , C.C. Sc h o n , size variation in diploid and tetraploid wild wheats. AoB S. Ta u d i e n , U. Sc h o l z , N. St e i n , K.F. Ma y e r & E. Ba u e r : From RNA- Plants 2010 (2010) plq015, doi:10.1093 / aobpla / plq015. seq to large-scale genotyping - genomics resources for rye Se č e n j i , M., Á. Le n d v a i , P. Mi s k o l c z i , G. Ko c s y , Á. Ga l l é , A. Sz u c s , B. (Secale cereale L.). BMC Plant Biol. 11 (2011) 131. Ho f f m a n n , É. Sá r v á r i , P. Sc h w e i z e r , N. St e i n , D. Du d i t s & J. Gy ö r g y e y : Liu, H., J. McNi c o l , M. Ba y e r , J.A. Mo r r i s , L. Ca r d l e , D.F. Ma r s h a l l , Differences in root functions during long-term drought D. Sc h u l t e , N. St e i n , B.J. Sh i , S. Ta u d i e n , R. Wa u g h & P.E. 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Kilian, J. Ro d e & K. C.C. Wu, A. Gr a n e r , P. La n g r i d g e & N. St e i n : BAC library resources Pi l l e n : Association of barley photoperiod and vernalization for map-based cloning and physical map construction in genes with QTLs for flowering time and agronomic traits in barley (Hordeum vulgare L.). BMC Genomics 12 (2011) 247. a BC2DH population and a set of wild barley introgression Sc h w e i z e r , P. & N. St e i n : Large-scale data integration reveals co- lines. Theor. Appl. Genet. 120 (2010) 1559-1574. localization of gene functional groups with meta-QTL for Wa u g h , R., D. Ma r s h a l l , B. Th o m a s , J. Co m a d r a n , J. Ru s s e l l , T. Cl o s e , N. multiple disease resistance in barley. Mol. Plant Microbe St e i n , P. Ha y e s , G. Mu e h l b a u e r , J. Co c k r a m , D. O’Su l l i v a n , I. Ma c k a y , Interact. 24 (2011) 1492-1501. A. Fl a v e l l & L. Ra m s a y : Whole-genome association mapping Sh a r m a , S., S. Sh a r m a , F.J. Ko p i s c h -Ob u c h , T. Ke i l , E. La u b a c h , N. St e i n , in elite inbred crop varieties. Genome 53 (2010) 967-972. A. Gr a n e r & C. Ju n g : QTL analysis of root-lesion nematode Ye d i a y , F.E., F.S. Ba l o c h , B. Kilian & H. Öz k a n : Testing of rye-specific resistance in barley: 1. Pratylenchus neglectus. Theor. Appl. markers located on 1RS chromosome and distribution of Genet. 122 (2011) 1321-1330. 1AL.RS and 1BL.RS translocations in Turkish wheat (Triticum Ta u d i e n , S., B. St e u e r n a g e l , R. Ar i y a d a s a , D. Sc h u l t e , T. Sc h m u t z e r , aestivum L., T. durum Desf.) varieties and landraces. Genet. M. Gr o t h , M. Fe l d e r , A. Pe t z o l d , U. Sc h o l z , K.F. Ma y e r , N. St e i n Resour. Crop Evol. 57 (2010) 119-129. & M. Pl a t z e r : Sequencing of BAC pools by different next generation sequencing platforms and strategies. BMC Res. 2011 Notes 4 (2011) 411. An d e d e n , E.E., F.E. Ye d i a y , F.S. Ba l o c h , S. Sh a a f , B. Kilian, M. Na c h i t Tu b e r o s a , R., A. Gr a n e r & R.K. Va r s h n e y : Genomics of plant genetic & H. Öz k a n : Distribution of vernalization and photoperiod resources: an introduction. Plant Genet. Resour. 9 (2011) genes (Vrn-A1, Vrn-B1, Vrn-D1, Vrn-B3, Ppd-D1) in Turkish 151-154. bread wheat cultivars and landraces. Cereal Res. Commun. Wi c k e r , T., K.F.X. Ma y e r , H. Gu n d l a c h , M. Ma r t i s , B. St e u e r n a g e l , U. 39 (2011) 352-364. Sc h o l z , H. Ši m k o v á , M. Ku b a l á k o v á , F. Ch o u l e t , S. Ta u d i e n , M. Co m a d r a n , J., L. Ra m s a y , K. Ma c k e n z i e , P. Ha y e s , T.J. Cl o s e , G. Pl a t z e r , C. Fe u i l l e t , T. Fa h i m a , H. Bu d a k , J. Do l e ž e l , B. Ke l l e r & N. Mu e h l b a u e r , N. St e i n & R. Wa u g h : Patterns of polymorphism St e i n : Frequent gene movement and pseudogene evolution and linkage disequilibrium in cultivated barley. Theor. Appl. is common to the large and complex genomes of wheat, Genet. 122 (2011) 523-531. barley, and their relatives. Plant Cell 23 (2011) 1706-1718. Dr u k a , A., J. Fr a n c k o w i a k , U. Lu n d q v i s t , N. Bo n a r , J. Al e x a n d e r , K. Ye d i a y , F.E., E.E. An d e d e n , F.S. Ba l o c h , A. Bö r n e r , B. Kilian & H. Öz k a n : Ho u s t o n , S. Ra d o v i c , F. Sh a h i n n i a , V. Ve n d r a m i n , M. Mo r g a n t e , N. The allelic state at the major semi-dwarfing genes in a panel St e i n & R. Wa u g h : Genetic dissection of barley morphology of Turkish bread wheat cultivars and landraces. Plant Genet. and development. Plant Physiol. 155 (2011) 617-627. Resour. 9 (2011) 423-429. Ha r t m a n n , A., T. Cz a u d e r n a , R. Ho f f m a n n , N. St e i n & F. Sc h r e i b e r : HTPheno: An image analysis pipeline for high-throughput plant phenotyping. BMC Bioinformatics 12 (2011) 148.

31 Abteilung Genbank/Department of Genebank

Books and Book Chapters Or d o n , F. & A. Gr a n e r : PCR in der Pflanzenzüchtung: Resistenz gegen die Gelbmosaikvirose der Gerste. Biospektrum 5 2010 (2010) 539-542. Kilian, B., W. Ma r t i n & F. Sa l a m i n i : Genetic diversity, evolution and Tu b e r o s a , R. & A. Gr a n e r : Second Congress on Genomics of Plant domestication of wheat and barley in the Fertile Crescent. In: Genetic Resources (GPGR2). Biovers. Newslett. Europe 40 Gl a u b r e c h t , M. (Ed.): Evolution in Action. Springer Publishers, (2010) 10. New York (2010) 137-166. Sh a r m a , S. & R. Sh a r m a : Biotechnology and sustainable agriculture 2011 marker assisted selection. In: Se n g a r , R.S. & A.K. Sh a r m a (Eds.): Gr a n e r , A., G. Ha s e n e y e r , B. Kilian, R.K. Pa s a m , R. Sh a r m a & N. Stable Food Production and Sustainable Agriculture: A St e i n : Nutzung genetischer Vielfalt: Herausforderung und Challenge Ahead in 21st Century. Studium Press (2010) 335- Perspektiven. AgrarSpektrum 44 (2011) 45-61. 351.

2011 PhD and Diploma Theses Gr a n e r , A., A. Kilian & A. Kl e i n h o f s : Barley genome organization, 2011 mapping and synteny. In: Ul l r i c h , S.E. (Ed.): Barley: Production, Jo s t , M.: Genetische Kartierung der Ährenmorphologie – Improvement, and Uses. Blackwell Publishing Ltd., Oxford/ Mutante laxatum.a in Gerste. (Diploma Thesis) Martin- UK (2011) 63-84. Luther-Universität Halle-Wittenberg, Halle/S. (2011) 69 pp. Kilian, B., K. Ma m m e n , E. Mi l l e t , R. Sh a r m a , A. Gr a n e r , F. Sa l a m i n i , K. We i s s g e r b e r , W.: Evolution der Spindelbrüchigkeit bei Gerste. Ha m m e r & H. Öz k a n : Aegilops L. In: Ko l e , C. (Ed.): Wild Crop (Diploma Thesis) Martin-Luther-Universität Halle- Relatives: Genomic and Breeding Resources: Cereals. Wittenberg, Halle/S. (2011) 152 pp. Springer-Verlag, Berlin Heidelberg (2011) 1-76.

Additional Publications 2009 Other Papers Tu r u s p e k o v , Y., I. Ho n d a , Y. Wa t a n a b e , N. St e i n & T. Ko m a t s u d a : An 2010 inverted and micro-colinear genomic region of rice and Gr a n e r , A.: Research along the value chain of biodiversity. Public barley carrying the cly1 gene for cleistogamy. Breed. Sci. 59 Service Review. Sci. Technol. 7 (2010) 160-161. (2009) 657-663. Gr a n e r , A.: Begrüßung. In: Wo b u s , A.M., U. Wo b u s & B. Pa r t h i e r (Eds.): Der Begriff der Natur. Wandlungen unseres Naturverständnisses und seine Folgen. Gaterslebener Patents Begegnung 2009. Nova Acta Leopoldina, Bd. 109, Heft 376, 2010 Wissenschaftliche Verlagsgesellschaft, Stuttgart (2010) Go t t w a l d , S. & N. St e i n : “Six-row-type barley”. WO 2010/076279; 9-11. Anmeldetag: 02.01.2009, Offenlegung: 07.07.2010, IPK Nr. 2008/08.

32 Research Group: Genebank Documentation Head: Dr. Helmut Knüpffer

Scientists activities. The module for handling characterisation and evaluation (C&E) data of genebank accessions was completed. IPK financed This includes interaction with the pocket PCs used for C&E Oppermann, Markus data recording in the field (GBIS/B), as well as visualisation, Stephanik, Andreas (till 31.01.2010) editing and searching C&E data via GBIS/M (A. Stephanik, M. Oppermann). The development of the in vitro storage module Visiting Scientists/Scholars (Fig. 13) and the complete integration of the Groß Lüsewitz Jinjikhadze, Tamar (Tbilisi Botanical Garden, 19.03.-29.03.2011) potato collection (Satellite Collections North) in GBIS started Stephanik, Andreas (self-financed, 01.02.-31.12.2010) in 2010 (C. Dittmann, M. Oppermann, cooperation with K. Dehmer). The Allium Taxonomic Reference Collection of the research group Taxonomy of Plant Genetic Resources (R. Fritsch) Goals was fully integrated in GBIS (M. Oppermann). The developers’ (technical) documentation for GBIS/M Development and maintenance of information systems for plant and the user manual are updated annually. Bugfixing, the genetic resources (PGR) with the aim to provide information on implementation of change requests, and improving the user- PGR to researchers, breeders and other users, and to support friendliness of GBIS are continuously carried out. The data base the management of genebank material. was increased and further consolidated, and the data quality was improved (M. Oppermann, H. Knüpffer).

Research Report (2) GBIS/I, the internet portal for searching and ordering genebank accessions (http://gbis.ipk-gatersleben.de/gbis_i/), The group’s activities were focused on the continuous provides online access to 148,500 accessions of the IPK Genebank maintenance, development and extension of the Genebank collections at Gatersleben, Malchow and Groß Lüsewitz. It is Information System (GBIS). GBIS is being developed in an being used by 75 % of the users for material requests. Oracle environment. Integration of IPK data in information networks. Passport (1) GBIS/M, the internal genebank management system, offers data of genebank accessions were repeatedly exported and numerous functions supporting the day-to-day genebank submitted to the German National Inventory of PGR (PGRDEU),

Fig. 13 The GBIS/M project “In vitro Management” aims at integrating the in vitro preservation activities of the research groups In vitro Storage und Cryopreservation and Satellite Collections North into the Genebank Information System GBIS/M. The background figure depicts an in vitro storage room. The image on the bottom left shows the extended management system of GBIS/M. On the right side, the three daily work processes, i.e. “In vitro cultivation/Splitting”, “Relocation” and “Health tests” are shown (Photo: IPK Gatersleben/C. Dittmann).

33 Abteilung Genbank/Department of Genebank

the European Central PGR Search Catalogue (EURISCO), and 2011 numerous ECPGR European Central Crop Databases. GBIF En d r e s e n , D.T.F. & H. Kn ü p f f e r : The Darwin Core extension (Global Biodiversity Information Facility) is accessing IPK’s for genebanks opens up new opportunities for sharing passport data via a web service. genebank datasets. In: Endresen, D.T.F.: Utilization of plant The group is presently updating the European Barley Database genetic resources. A lifeboat to the gene pool. (PhD thesis) (EBDB) (H. Knüpffer, M. Oppermann) and it is maintaining Faculty of Life Sciences, Univ. Copenhagen (2011) 121-142. the European Poa Database of the European Cooperative Programme for Plant Genetic Resources (ECPGR), the latter together with E. Willner (Satellite Collections North). Via an Electronic Publications interface, passport and observation data from GBIS are accessed 2010 by the Garlic and Shallot Core Collection Database (GSCC) Hö p p n e r , F. & H. Kn ü p f f e r : Status report on the German collections (J. Keller, research group In vitro Storage and Cryopreservation, of flax and hemp. Report, ECP/GR Fibre Crops (Flax and in cooperation with research group Bioinformatics and Hemp) Working Group 1st meeting, Wageningen, The Information Technology) and by the Diversity Studies Toolkit Netherlands, 14 – 16 June 2006. http://www.ecpgr.cgiar.org/ 2 (DISTo2) developed by the research group Bioinformatics and Workgroups/Flax_Hemp/Flax%20Presentations%202006/ Information Technology for the Lolium collection. Germany_Country_report_final.pdf (2010). Op p e r m a n n , M., J. Ke i l w a g e n , H. Kn ü p f f e r , S. Fr i e d e l & A. Bö r n e r : The group is also maintaining other databases: Mansfeld’s World Validation, analysis and aggregation of long-term trait Database of Agricultural and Horticultural Crops (http:// observation data of genebank material. In: We i t z m a n n , mansfeld.ipk-gatersleben.de) and the in-house Database for A.L. (ed.), Proceedings of TDWG (2010). Woods Hole, Checklists of Cultivated Plants (not online) (M. Oppermann, H. Massachusetts, USA (2010) 52-53. http://www.vliz.be/ Knüpffer). imisdocs/publications/215525.pdf (2010). The automatic IPK weather stations in Gatersleben and We i s e , S., N. Bi e r m a n n , S. Fl e m m i n g , J. Vo r w a l d , T. v a n Hi n t u m , H. Kn ü p f f e r Malchow (since 2011) are operated by the group, and the data & I. Gr o s s e : Proposal for improvement of PGR data exchange generated are made publicly accessible via the IPK website through XML. Proceedings of the EPGRIS Final Conference, (M. Oppermann). 11-13 September 2003, Prague, Czech Republic. International Plant Genetic Resources Institute (IPGRI). http://www.ecpgr. cgiar.org/Networks/Info_doc/FinalMeeting Presentations/ Publications Papers/Bierman_IPK_v0.14.doc (2010). We i s e , S., S. Ha r r e r , I. Gr o s s e , H. Kn ü p f f e r & E. Wi l l n e r : The European Peer Reviewed Papers Poa Database (EPDB). http://poa.ipk-gatersleben.de (2010).

2010 2011 Kn ü p f f e r , H.: The Balkan Collections 1941-1942 of Hans Stubbe Kn ü p f f e r , H., L. Ma g g i o n i , M. Ja l l i , A. Ko l o d i n s k a , D. Fa s o u l a & E. Li p m a n : in the Gatersleben Gene Bank. Czech J. Genet. Plant Breed. Report of a working group on barley. Seventh meeting, 10- 46 (2010) S27-S33. 12 May 2011, Nicosia, Cyprus. Bioversity International, Rome, v a n Hi n t u m , T. & H. Kn ü p f f e r : Current taxonomic composition Italy. ii+43 pp. http://www.ecpgr.cgiar.org/fileadmin/www. of European genebank material documented in EURISCO. ecpgr.cgiar.org/NW_and_WG_UPLOADS/Barley_7_Cyrpus/ Plant Genet. Resour. 8 (2010) 182-188. Barley_7_Cyprus_revised210911.pdf, (2011) Ma g g i o n i , L., A. Ka t s i o t i s , H. Kn ü p f f e r , G. Kl e i j e r & E. Li p m a n : Report of 2011 a Cereals Network. Second Meeting, 21-24 April 2008, Foça, Hovhannisyan , N.A., M.E. Du l l o o , A.H. Ye s a y a n , H. Kn ü p f f e r & A. Am r i : Turkey. May 2011. Bioversity International, Rome, Italy (2011) Tracking of powdery mildew and leaf rust resistance genes iv+64 pp. http://www.ecpgr.cgiar.org/fileadmin/bioversity/ in Triticum boeoticum and T. urartu, wild relatives of common publications/pdfs/1437_Report%20of%20a%20cereals%20 wheat. Czech J. Genet. Plant Breed. 47 (2011) 45-57. network%20second%20meeting%2021-24%20April%20 2008%20Fo%C3%A7a%20Turkey.pdf?cache=1304494577 (2011). Other Papers We i s e , S., S. Ha r r e r , I. Gr o s s e , H. Kn ü p f f e r & E. Wi l l n e r : The European Poa Database (EPDB). http://poa.ipk-gatersleben.de (2011). 2010 Kn ü p f f e r , H.: Plant genetic resources from Greece preserved in the German Genebank in Gatersleben, with emphasis on Additional Publications 2009 Hans Stubbe’s Balkan collections in 1941-1942. Proceedings Hovhannisyan , N., M.E. Du l l o o , A. Ye s a y a n , M.S. Rö d e r , H. Kn ü p f f e r , of the 12th Panehellenic Congress of the Hellenic Scientific A. Am r i & A.M. Da n i e l i a n : Wild relatives of wheat as sources of Society of Plant Breeding & Genetics, 8 - 10 October 2008, useful genes. In: In Vitro Cellular & Developmental Biology- Naoussa, Greece, CD-ROM, (2009) 16-29. Animal. Vol. 45, Springer, New York (2009) S82-S83.

34 Research Group: Plant Architecture Head: Dr. Thorsten Schnurbusch

Scientists Research Report

IPK financed Cereal grains are produced in specific plant organs called ears Seidensticker, Tina (0.50) or spikes. Spike development of cereal crop plants, such as barley (Hordeum vulgare L.) or wheat (Triticum aestivum L.), is Grant Positions one of the important determinants of reproductive success and Alqudah, Ahmad Mohammad (0.65 DFG, since 28.09.2010) is measurable as grain yield. Modern wheat cultivars typically Bini, Federica, Dr. (0.75/0.50 BMBF, 01.02.-31.12.2010; 0.25/0.50 have a 10 to 15 cm long spike with 17 to 25 spikelets attached Overhead, 01.11.2010-30.06.2011) to the rachis. Each spikelet ‑ the actual inflorescence‑ is able to Gawroński, Piotr (0.50 DFG) produce two to six seeds; each seed contained in florets.C ereal Koppolu, Ravi (0.50 DFG, since 16.03.2010) grains contributed more than 50 % of all food calories world- Youssef, Helmy Mohamed, Dr. (0.50 Overhead, wide in 2006 (FAO Statistics 2008) and bread wheat represents 01.02.-31.05.2011) the major cereal crop in Germany, where it is usually cultivated on more than 3 Mio ha annually. However, despite these facts Visiting Scientists/Scholars our understanding of the developmental genetics of central Bini, Federica, Dr. (self-financed, since 01.09.2011) features such as spike morphology and development is very Fazeli, Arash (Ministry of Science, Research and Technology, limited. Functional knowledge of genes, which regulate key Iran, 13.04.-12.10.2011; self-financed, 13.10.-31.12.2011; morphological and developmental traits such as inflorescence IPK, 01.01.-29.02.2012) architecture, spikelet initiation or abortion, rachis internode Shavrukov, Yuri, Dr. (self-financed, 19.05.-21.05.2010) length, or total number of rachis internodes is almost completely Youssef, Helmy Mohamed, Dr. (DAAD, 09.05.-09.07.2010) lacking in most of our cereal crops.

In the BMBF-funded project (GABI-FUTURE Start: T. Schnur- Goals busch; T. Seidensticker) we are performing detailed phenotypic analyses of spike mutants in wheat and barley with Elucidating the developmental and molecular genetics of spike a specific interest in spikelet formation and identity, as well as development in small grain cereals wheat and barley. spike branching. The latter feature can be classified into three

Fig. 14 Spikelet development in standard wheat and ‘Miracle Wheat’, including the development of supernumerary spikelets at floret primordium stage (Photo: IPK Gatersleben).

35 Abteilung Genbank/Department of Genebank

phenotypic classes based upon their corresponding branch- The appearance of the six-rowed spike phenotype in barley is ing phenotypes: (I) the hetero-branched spike possess two or one of the important developmental processes that promoted more fully developed spikes which jointly emerge from the top barley domestication owing to its increased grain yield. Until of the peduncle; however, such mutant form is not part of our today, five different loci have been identified which can convert collection; (II) the pseudo-branched spike is a distinct feature of two-rowed barley to six-rowed barley; they include vrs1 (2HL), hexaploid T. vavilovii (Tumanian) Jakubz. and shows an exten- vrs2 (5HL), vrs3 (1HL), vrs4 (3HS) and int-c (5HS). Komatsuda et al. sion of rachilla internodes; and (III) the branched spike, mainly (PNAS 2007, 104:1424–1429) identified the Vrs1 gene as being occurring in tetraploid wheats (T. turgidum L. convar. composi- an HD ZIP transcription factor and recently Ramsay et al. found tum (L.f.) A. Filat.) and sometimes also known as “Miracle” or int-c as the barley orthologue of maize Teosinte Branched1 (Nat. “Egyptian Wheat”, “Wunderweizen” or “Blé d’Osiris”, develops Genet. 2011, 43:169-172). Among other vrs loci (vrs2 and vrs3) first order branches with additional, completely fertile spikelets vrs4 is known to produce a prominent six-rowed phenotype with preferentially at the base of the spike (Fig. 14, p. 35). Depending many fully fertile, long awned lateral spikelets. In our present on the genotype and environmental conditions, the degree of DFG-funded project (R. Koppolu) we mapped the vrs4 locus in branching can be reduced, and hence, only additional spikelets two bi-parental mapping populations (Barke × vrs4.k and vrs4.k develop from one rachis node showing so called double, twin × Golden Promise; 96 individuals in each), using SNP-based or supernumerary spikelets. The branched-spike phenotype is CAPS and VeraCode markers, thereby applying the following of particular interest to our research group since it might de- research approach. The phenotype showed linkage to markers liver one approach for increasing spikelet numbers per spike in derived from chromosome 3HS. The corresponding marker- wheat by modulating an important grain yield component. De- phenotype interval comprised 27 genes in Brachypodium, velopmentally, grain yield potential in current wheat cultivars annotations of which revealed an important transcription factor is primarily influenced through the floret fertility per spikelet, involved in inflorescence development. Re-sequencing of the i.e. whether two, three, four or more grains per spikelet develop. transcription factor in vrs4.k and its wild type MFB 104 showed During the life cycle of wheat plants, the number of spikelets a unique deletion in the vrs4.k mutant, resulting in a truncated per spike is rather early determined, namely once the terminal gene product. Hence, we re-sequenced the gene in 18 vrs4 spikelet has been formed. After terminal spikelet formation, no mutant alleles available from NordGen, Sweden and USDA, USA; additional spikelets are formed, and thus, grain yield poten- most of them showed nucleotide changes in the coding region, tial is mainly regulated through floret fertility. However, in the but also in upstream or downstream regions of the gene. Fine branched wheats this determinacy of spikelet development is mapping and the establishment of a physical contig for the usually not seen; a terminal spikelet develops only occasionally. vrs4 locus is already underway. Tissue localisation of vrs4 gene Additional spikelets are still being formed on first order branch- expression through in situ hybridisations, its genetic networks es, hence commonly increasing total grain number per spike but by microarray analysis and a working model for the six-rowed in turn reducing the number of grains per spikelet and grain size. spike pathway, involving vrs4, will soon become available. Nevertheless, elucidating the genetic and molecular factors, influencing spikelet development and spike branching in “Miracle Wheats” is one central goal of our research group.

Fig. 15 Predominantly applied research approach within the research group Plant Architecture during the gene isolation process.

36 In cereal breeding, optimal adaptation to a given environment Publications and subsequently high yield potential is mainly determined by the time of flowering. Flowering time, however, is commonly (The publication with a “*” is based on work that has been carried affected by a complex interplay between three determinants: out when Thorsten Schnurbusch was at the University of photoperiodic and vernalisation requirements as well as Adelaide, Australian Centre for Plant Functional Genomics, the intrinsic capability of a cultivar/genotype to flower. In Australia). temperate grasses, such as wheat and barley, earliness per se (Eps) is understood as the intrinsic difference in flowering time Peer Reviewed Papers of fully vernalised plants grown under long day conditions. In the current DFG-funded project (P. Gawroński), two einkorn 2010 wheat lines, RIL25 (early) and RIL71 (late), were selected from *Sc h n u r b u s c h , T., J. Ha y e s , M. Hr m o v a , U. Ba u m a n n , S.A. Ra m e s h , S.D. a RILWA1 population (Triticum monococcum L. × T. boeoticum Ty e r m a n , P. La n g r i d g e & T. Su t t o n : Boron toxicity tolerance in Boiss.) to generate a new F2 population for fine mapping of barley through reduced expression of the multifunctional the Eps-3Am locus. About 650 F2 individuals were screened for aquaporin HvNIP2;1. Plant Physiol. 153 (2010) 1706-1715. genetic recombinations and new markers were added utilising Sc h n u r b u s c h , T., J. Ha y e s & T. Su t t o n : Boron toxicity tolerance in the physical map from barley chromosome 3H. The locus could wheat and barley: Australian perspectives. Breed. Sci. 60 be delimited to ca. 350 kb and contained only two putative (2010) 297-304. genes. Moreover, both genes were found to be deleted in the mutant parent of the RILWA1 population KT3-5 (T. monococcum L.) as well as in RIL25. One of the two genes deleted in the KT3- PhD and Diploma Theses 5 mutant belonged to the circadian clock which is known to regulate the photoperiodic flowering pathway. Therefore, two 2011 experiments were performed to verify whether circadian clock Ed w a r d s , J.: A genetic analysis of drought related traits in distortions could be observed: (i) results received from delayed hexaploid wheat. (PhD Thesis) School of Agriculture, fluorescence measurements indicated that the mutant KT3- Food and Wine, Discipline of Plant Breeding and Genetics 5 had indeed an affected rhythm of the chloroplastic clock Australian Centre for Plant Functional Genomics, The compared with wild type line KT3-1. And (ii) secondly, obtained University of Adelaide, Australia (2011) 233 pp. gene expression data from a time-course qRT-PCR experiment on two key nuclear clock genes, TmTOC1 (timing of CAB2 expression 1) and TmLHY (late elongated hypocotyl), supported our hypothesis that the Eps-3Am locus had been affected in a gene belonging to the circadian clock.

37 Abteilung Genbank/Department of Genebank

Programme: Management and Evaluation Research Group: Resources Genetics and Reproduction Head: Dr. Andreas Börner

Scientists The studies of the collection-related research on seed longevity in barley were continued. In cooperation with the research IPK financed group Genome Diversity (N. Stein, B. Kilian) a highly saturated Lohwasser, Ulrike, Dr. bi-parental mapping population together with an association Nagel, Manuela, Dr. (0.50, till 30.06.2011) mapping panel were investigated by exploiting experimental ageing tests following the rules of the International Seed Testing Grant Positions Association (M. Nagel, M. Gäbler, I. Daniel). The studies revealed Nagel, Manuela, Dr. (ICARDA / GTZ, 01.07.-30.11.2011) loci which are assumed to be responsible for the disruption Pochepnya, Nadezhda (0.50 ICARDA / GTZ, 18.10.-31.12.2010) of the cellular homeostasis that enhances the production of reactive oxygen species. The major antioxidant glutathione Visiting Scientists/Scholars (GSH) is generally affected by these stresses and was analysed Allam, Mai (DAAD, since 04.04.2011) in 26 accessions which were maintained in parallel at cold (0 °C) Danesh-Shahraki, Abdolrazagh, Dr. (University of Shahrekord, and ambient (20 °C) storage up to 15 years. Further six of them 13.07.-29.07.2010) (harvested in 2008) were experimentally aged with low (13 %) Daniel, Isaac, Dr. (Humboldt Foundation, 01.07.-26.09.2011) and high (18 %) seed moisture content (smc). In all storage Dobrovolskaya, Oxana, Dr. (DFG, 02.03.-03.04.2010; 31.10.- treatments, a general depletion of the total glutathione pool 31.12.2010) and a shift towards more oxidising conditions, expressed by Jantassov, Serik (GIZ Bonn / Eschborn, 11.09.-17.09.2011) the oxidised glutathione (GSSG), were seen with ageing (see Kruppa, Klaudia (Agricultural Research Institute Martonvásár, Fig. 16A, p. 39). The drier seeds of ambient and cold storage 10.01.-09.04.2011) had a higher GSSG concentration than the moister seeds Neumann, Kerstin (self-financed, till 31.08.2010) exposed to artificial ageing, suggesting that enzymes were Rehman Arif, Mian Abdur (DAAD) not active in the dry state to reduce GSSG to GSH. Ageing at Stimolo, Lucia (self-financed, 23.08.-27.08.2010) 18 % smc was less destructive and enzymes could synthesise Tikhenko, Natalia, Dr. (self-financed, 04.04.-14.04.2010) further GSH. Comparing six barley genotypes over different Weidner, Annette, Dr. (self-financed, till 30.06.2010) storage and ageing treatments showed that genotypes Zaynali Nezhad, Khalil (Iranian Government, till 30.09.2010) maintained germination but with different concentrations of total GSH which is assumed to be specific to the genotype and may depend on the pre-harvest conditions. Therefore highly Goals significant correlations over all treatments were not found for GSH but for percentage of GSSG of the total glutathione pool

Long-term seed storage; reproduction, distribution, evaluation and the half-cell reduction potential (EGSSG / 2GSH) which both and genetic characterisation of genebank collections. represent good viability markers. Testing the same material on tocopherols, (see Fig. 16B) and storage compounds namely oil content, starch and proteins (see Fig. 16C) revealed that Research Report these metabolites were not affected by ageing treatments (M. Nagel; collaboration with H. Rolletschek, research group The total number of accessions maintained at the Gatersleben Heterosis). site comprises 130,620 samples, of which 123,147 are preserved in the cold store. Safety duplicates are available for 29,963 Recently the genetic studies on seed longevity were extended accessions (ca. 20 % of the whole collection). They are stored to wheat and oilseed rape (M.A. Rehman Arif, M. Allam). For at the Global Seed Vault, Svalbard, Norway. In the reporting wheat an association mapping approach was initiated using period germination tests were performed for 21,122 samples long-term stored (35 years) accessions. The variation of seed (S. Pistrick). 48,043 accessions (excluding the Satellite Collections germination (longevity) ranged between 0 and 94 %. The North) were distributed to users. During the regeneration accessions were regenerated and experimentally aged in seasons 2009/2010 and 2010/2011 totals of 8,819 and 10,654 order to compare loci for seed longevity detected after long- accessions were cultivated, respectively, including 772 and 523 term storage and accelerated ageing approach. Only a few samples grown for evaluation only (M. Grau, M. Kotter, K. Krusch, R. common loci were discovered. It was concluded that different Kurch, B. Schmidt in collaboration with P. Schreiber, Experimental deterioration mechanisms may be involved in experimental Fields and Nurseries). A taxonomic classification was performed ageing and conventional long-term cold storage. for 8,588 accessions. Descriptor lists were created or revised for species of the genera Anethum, Apium, Brassica, Crambe, Pastinaca, Research to improve the utilisation of the cereal genebank Phaseolus, Raphanus, Sinapis and Vicia (U. Lohwasser). collection is focused on agronomic traits but mainly abiotic

38 Activities on characterisation and evaluation directed to Petroselinum were continued. The taxonomic classification based on morphological characters was verified. Using molecular markers root parsleys form a cluster together with some smooth leaf types. The other smooth and curled parsleys appear in a second cluster. Analysing essential oil compounds the content of myrcene and β-pellandrene are correlated with root and leaf parsley, respectively (R. Kurch, U. Lohwasser). A European Project on conservation and utilisation of leafy vegetables (Lactuca, Spinacia, Cichorium, Valerianella, Eruca, Diplotaxis) was successfully completed. The European- wide activities on regeneration and characterisation were coordinated by IPK (S. Thumm, K. Krusch, U. Lohwasser).

Publications

Peer Reviewed Papers

2010 Bö r n e r , A., K. Ne u m a n n , B. Ko b i l j s k i & U. Lo h w a s s e r : Association mapping for flowering time in wheat using an ex situ core collection. Agriculturae Conspectus Scientificus 75 (2010) 141-144. Ca s t i l l o , A., H. Bu d a k , C. Ma r t í n , G. Do r a d o , A. Bö r n e r , M. Rö d e r & P. He r n a n d e z : Interspecies and intergenus transferability of barley and wheat D-genome microsatellite markers. Ann. Appl. Biol. 156 (2010) 347-356. Kh l e s t k i n a , E.K., M.S. Rö d e r & A. Bö r n e r : Mapping genes controlling anthocyanin pigmentation on the glume and pericarp in Fig. 16 Relationship between antioxidants, storage compounds and seed longevity. tetraploid wheat (Triticum durum L.). Euphytica 171 (2010) A) Glutathione (GSH, white bars) and the redox partner glutathionedisulphide 65-69. (GSSG, black bars), are shown in relation to total germination (blue squares). B) α (green dot) β & γ purple dot) and δ (yellow dot). Tocopherol contents are shown Kh l e s t k i n a , E.K., M.S. Rö d e r , T.A. Pshenichnikova & A. Bö r n e r : and do not undergo changes during storage and artificial ageing.C ) Seed storage reserves of protein (yellow square), oil (black square) and starch (grey square) did Functional diversity at the Rc (red coleoptile) gene in bread not change significantly in response to any treatment (M. Nagel). wheat. Mol. Breed. 25 (2010) 125-132. Kr ü g e r , H., W. Sc h ü t z e , U. Lo h w a s s e r & F. Ma r t h e : Qualität bei Melisse – gestern und heute, Hydroxyzimtsäurederivate stress tolerance (drought, pre-harvest sprouting) in barley and versus Rosmarinsäure, vergleichende Untersuchungen wheat. Loci determining the traits of interest were detected on an einer Melissekollektion. Zeitschr. Arznei-Gewürzpfl. 15 different chromosomes via segregation (collaboration with (2010) 31-32. M. Röder, research group Gene and Genome Mapping) and La m i e n -Me d a , A., M. Ne l l , U. Lo h w a s s e r , A. Bö r n e r , C. Fr a n z & J. No v a k : association mapping. For drought tolerance loci (marker trait Investigation of antioxidant and rosmarinic acid variation in associations) were detected on barley chromosomes 3HL, the sage collection of the genebank in Gatersleben. J. Agr. 4HL, 5HL and 6HS in regions carrying dehydrin (Dhn) genes. Food Chem. 58 (2010) 3813-3819. The dehydrin multigenes are known as regulatory genes for La m i e n -Me d a , A., C. Sc h m i d e r e r , U. Lo h w a s s e r , A. Bö r n e r , C. Fr a n z & J. cold and drought stress tolerance (K. Neumann). Loci for pre- No v a k : Variability of the essential oil composition in the sage harvest sprouting/dormancy were detected on all seven collection of the Genebank Gatersleben: a new viridiflorol homoeologous groups of wheat and on the chromosomes chemotype. Flavour Frag. J. 25 (2010) 75-82. 2H, 3H, 5H, 6H, and 7H of barley. Already known regions on La n d j e v a , S., U. Lo h w a s s e r & A. Bö r n e r : Genetic mapping within the chromosomes 3A and 4A for wheat and 5H for barley were wheat D genome reveals QTL for germination, seed vigour confirmed.C andidates as viviparous1 gene on chromosome 3A, and longevity, and early seedling growth. Euphytica 171 a protein belonging to the aquaporin family on chromosome 4A (2010) 129-143. and the aleurain gene on chromosome 5H may be responsible Le o n o v a , L.N., E.B. Bu d a s h k i n a , K. Fl a t h , A. We i d n e r , A. Bö r n e r & M. for the characters investigated (U. Lohwasser, M.A. Rehman Arif, Rö d e r : Microsatellite mapping of a leaf rust resistance gene A. Marlow, S. Thumm). transferred to common wheat from Triticum timopheevii. Cereal Res. Commun. 38 (2010) 211-219.

39 Abteilung Genbank/Department of Genebank

Lo h w a s s e r , U., A. Di t t b r e n n e r , H. Bu d a h n , F. Ma r t h e & A. Bö r n e r : Kh l e s t k i n a , E.K., E.A. Sa l i n a , I.E. Ma t t h i e s , I.N. Le o n o v a , A. Bö r n e r & Taxonomy of plant genetic resources – use of morphological, M.S. Rö d e r : Comparative molecular marker-based genetic molecular and phytochemical data in order to verify existing mapping of flavanone 3-hydroxylase genes in wheat, rye classification.A griculturae Conspectus Scientificus 75 (2010) and barley. Euphytica 179 (2011) 333-341. 175-178. Le o n o v a , I.N., E.B. Bu d a s h k i n a , N.P. Ka l i n i n a , M.S. Rö d e r , A. Bö r n e r Lo h w a s s e r , U., T. St r u c k m e y e r , H. Bu d a h n , H. Kr ü g e r , D. Ul r i c h , & E.A. Sa l i n a : Triticum aestivum × Triticum timopheevii M. De c l e r c q , A. Bö r n e r & F. Ma r t h e : The German parsley introgression lines as a source of pathogen resistance genes. germplasm collection – interaction of morphological, Czech J. Genet. Plant Breed. 47 (2011) S49-S55. molecular and phytochemical characters. Acta Horticult. Na g e l , M., M. Ro s e n h a u e r , E. Wi l l n e r, R.J. Sn o w d o n , W. Fr i e d t & A. 860 (2010) 235-240. Bö r n e r : Seed longevity in oilseed rape (Brassica napus L.) - Ma d e r , E., U. Lo h w a s s e r , A. Bö r n e r & J. No v a k : Population structures genetic variation and QTL mapping. Plant Genet. Resour. 9 of genebank accessions of Salvia officinalis L. (Lamiaceae) (2011) 260-263. revealed by high resolution melting analysis. Biochem. Syst. Ne u m a n n , K., B. Ko b i l j s k i , S. De n c i c , R.K. Va r s h n e y & A. Bö r n e r : Ecol. 38 (2010) 178-186. Genome-wide association mapping – a case study in bread Na g e l , M. & A. Bö r n e r : The longevity of crop seeds stored under wheat (Triticum aestivum L.). Mol. Breed. 27 (2011) 37-58. ambient conditions. Seed Sci. Res. 20 (2010) 1-12. Os i p o v a , S.V., A.V. Pe r m y a k o v , M.D. Pe r m y a k o v a , V.A. Da v y d o v , T.A. Na v a k o d e , S., A. We i d n e r , R.K. Va r s h n e y , U. Lo h w a s s e r , U. Sc h o l z , M.S. Pshenichnikova & A. Bö r n e r : Tolerance of prolonged drought Rö d e r & A. Bö r n e r : A genetic analysis of aluminium tolerance among a set of bread wheat chromosome substitution lines. in cereals. Agriculturae Conspectus Scientificus 75 (2010) Cereal Res. Commun. 39 (2011) 343-351. 191-196. Os i p o v a , S.V., A.V. Pe r m y a k o v , M.D. Pe r m y a k o v a , M.V. Ko v a l e n k o v a , T.A. Sa l e m , K.F.M., R.K. Va r s h n e y , M.S. Rö d e r & A. Bö r n e r : EST-SSR Pshenichnikova & A. Bö r n e r : Leaf dehydroascorbate reductase based estimates on functional genetic variation in a barley and catalase activity is associated with soil drought tolerance (Hordeum vulgare L.) collection from Egypt. Genet. Resour. in bread wheat. Acta Physiol. Plant. 33 (2011) 2169-2177. Crop Evol. 57 (2010) 515-521. Sz i r a , F., A. Bö r n e r , K. Ne u m a n n , K.Z. Ne z h a d , G. Ga l i b a & A. Ba l i n t : Si m ó n , M.R., E.K. Kh l e s t k i n a , N.S. Ca s t i l l o & A. Bö r n e r : Mapping Could EST-based markers be used for the marker-assisted quantitative resistance to Septoria tritici blotch in spelt selection of drought tolerant barley (Hordeum vulgare) lines? wheat. Eur. J. Plant Pathol. 128 (2010) 317-324. Euphytica 178 (2011) 373-391. Ti k h e n k o , N., N. Ts v e t k o v a , A. Vo y l o k o v , O. Dobrovolskaya , K. Za y n a l i Ti k h e n k o , N., N. Ts v e t k o v a , S. Priyatkina, A. Vo y l o k o v & A. Bö r n e r : Ne z h a d , M.S. Rö d e r & A. Bö r n e r : Embryo lethality in wheat 3 Gene mutations in rye causing embryo lethality in hybrids rye hybrids - mode of inheritance and the identification of a with wheat – allelism test and chromosomal localisation. complementary gene in wheat. Euphytica 176 (2010) 191-198. Biol. Plant. 55 (2011) 448-452. Wi t z e l , K., A. We i d n e r , G.K. Su r a b h i , R.K. Va r s h n e y , G. Ku n z e , G.H. Bu c k - Wo r c h , S., K. Ra j e s h , V.T. Harshavardhan , C. Pi e t s c h , V. Ko r z u n , L. Ku n t z e , So r l i n , A. Bö r n e r & H.P. Mo c k : Comparative analysis of the grain A. Bö r n e r , U. Wo b u s , M.S. Rö d e r & N. Sreenivasulu : Haplotyping, proteome fraction in barley genotypes with contrasting linkage mapping and expression analysis of barley genes salinity tolerance during germination. Plant Cell Environ. 33 regulated by terminal drought stress influencing seed (2010) 211-222. quality. BMC Plant Biol. 11 (2011) 1. Ye d i a y , F.E., E.E. An d e d e n , F.S. Ba l o c h , A. Bö r n e r , B. Kilian & H. Öz k a n : 2011 The allelic state at the major semi-dwarfing genes in a panel Bö r n e r , A., K. Ne u m a n n & B. Ko b i l j s k i : Wheat genetic resources of Turkish bread wheat cultivars and landraces. Plant Genet. – how to exploit? Czech J. Genet. Plant Breed. 47 (2011) Resour. 9 (2011) 423-429. S43-S48. Da n i e l , I.O., M. Kr u s e & A. Bö r n e r : Comparative longevity and viability modeling of Solanum melongena seeds. Seed Sci. Books and Book Chapters Technol. 39 (2011) 680-685. Di a z d e Le o n , J.L., R. Escoppinichi , N. Ge r a l d o , A. Bö r n e r & M.S. Rö d e r : 2010 The performance of single chromosome substitution lines Fö r s t e r , K., U. Lo h w a s s e r & A. Bö r n e r (Eds.): Saatgut als Kulturerbe of bread wheat subjected to salinity stress. Cereal Res. – Produktion, Nutzung und Erhaltung. Berichte der Commun. 39 (2011) 317-324. Gesellschaft für Pflanzenbauwissenschaften, Bd. 5 (2010) Do b o s , G., R. Ku r c h , A. Bö r n e r & U. Lo h w a s s e r : Untersuchungen zur 139 pp. Winterfestigkeit von Schlafmohn (Papaver somniferum L.) der Genbank in Gatersleben. Zeitschr. Arznei- Gewürzpfl. 16 2011 (2011) 151-155. Ba l i n t , A., F. Sz i r a & A. Bö r n e r : Identification of loci affecting grain Kh l e s t k i n a , E.K., E.V. An t o n o v a , L.A. Pe r s h i n a , A.A. So l o v i e v , E.D. micronutrient content in cereals using association mapping. Ba d a e v a , A. Bö r n e r & E.A. Sa l i n a : Variability of Rc (red coleoptile) Agrisafe Final Conference “Climate change: Challenges and alleles in wheat and wheat-alien genetic stock collections. opportunities in agriculture” (21.-23.03.2011, Budapest/ Cereal Res. Commun. 39 (2011) 465-474. Hungary) (2011) 371-373.

40 Bö r n e r , A., E.K. Kh l e s t k i n a , S. Ch e b o t a r , M. Na g e l , M.A. Re h m a n -Ar i f , Da n i e l , I.O., M. Kr u s e , G. Mu l l e r & A. Bö r n e r : A MS Excel K. Ne u m a n n , B. Ko b i l j s k i , U. Lo h w a s s e r & M.S. Rö d e r : Maintenance implementation of the seed viability equation for managing and exploitation of genetic resources for future plant gene bank collections of Solanum melongena and Capsicum breeding. In: Ve i s z , O. (Ed.): Agrisafe Final Conference “Climate annuum. Proceedings of the XIVth EUCARPIA Meeting on change: Challenges and opportunities in agriculture” (21.- Genetics and Breeding of Capsicum & Eggplant, 30.08.- 23.03.2011, Budapest/Hungary). Agricultural Research 01.09.2010 Valencia, Spain, Universidad Politécnica de Institute of the Hungarian Academy of Sciences (2011) 26- Valencia, Valencia, Spain (2010) 37-47. 31. La n d j e v a , S., U. Lo h w a s s e r & A. Bö r n e r : Mapping QTL for Er m a k o v a , M.F., A.K. Ch i s t y a k o v a , L.V. Sh c h u k i n a , T.A. Pshenichnikova , germination, seed vigour and longevity on wheat D genome. E.V. Mo r o z o v a , A.V. Si m o n o v , A. We i d n e r & A. Bö r n e r : Berichte der Gesellschaft für Pflanzenbauwissenschaften, Technological properties of grain and flour in bread Bd. 5 (2010) 104-107. wheat lines with introgressions from Aegilops speltoides Lo h w a s s e r , U. & A. Bö r n e r : Saatgutqualität im Wandel der Zeit and Aegilops markgrafii. In: Ve i s z , O. (Ed.): Agrisafe Final – Von der Domestikation zum Qualitätsmanagement. Conference “Climate change: Challenges and opportunities Berichte der Gesellschaft für Pflanzenbauwissenschaften, in agriculture” (21.-23.03.2011, Budapest/Hungary). Bd. 5 (2010) 97-100. Agricultural Research Institute of the Hungarian Academy of Na g e l , M., I. Kr a n n e r & A. Bö r n e r : Langlebigkeit von Getreidesamen Sciences (2011) 63-66. und deren genetische Ursachen. Berichte der Gesellschaft Ne u m a n n , K., A.F. Ba l i n t , F. Sz i r a , M. Ba u m , R.K. Va r s h n e y & A. Bö r n e r : für Pflanzenbauwissenschaften, Bd. 5 (2010) 7-10. Genome-wide association mapping for early and late Na g e l , M., M. Re h m a n Ar i f , M. Ro s e n h a u e r & A. Bö r n e r : Longevity drought tolerance in a diverse barley collection. In: Ve i s z , O. of seeds ‑ intraspecific differences in the Gatersleben (Ed.): Agrisafe Final Conference “Climate change: Challenges genebank collections. Tagungsband der 60. Jahrestagung and opportunities in agriculture” (21.-23.03.2011, Budapest/ der Vereinigung der Pflanzenzüchter und Saatgutkaufleute Hungary). Agricultural Research Institute of the Hungarian Österreichs 2009 (2010) 179-181. Academy of Sciences (2011) 195-198. Re h m a n Ar i f , M.A., M. Na g e l , U. Lo h w a s s e r & A. Bö r n e r : Seed longevity Re h m a n -Ar i f , M.A., M. Na g e l , U. Lo h w a s s e r & A. B ö r n e r : Long-term seed in wheat (Triticum aestivum L.). Berichte der Gesellschaft für storability in genebank collections – genetic studies in wheat. Pflanzenbauwissenschaften, Bd. 5 (2010) 11-15. In: Ve i s z , O. (Ed.): Agrisafe Final Conference “Climate change: Ro s e n h a u e r , M., M. Na g e l , E. Wi l l n e r, F.-G. Sc h r ö d e r , R. Sn o w d o n , W. Challenges and opportunities in agriculture” (21.-23.03.2011, Fr i e d t & A. Bö r n e r : Genetische Untersuchungen des Merkmals Budapest/Hungary). Agricultural Research Institute of the Langlebigkeit von Saatgut der Spezies Brassica napus L. Hungarian Academy of Sciences (2011) 102-105. Berichte der Gesellschaft für Pflanzenbauwissenschaften, Sz i r a , F., A. Bö r n e r , K. Ne u m a n n , K.Z. Ne z h a d , G. Ga l i b a & A.F. Ba l i n t : Bd. 5 (2010) 108-111. EST-based markers associated with QTLs for drought Th u m m , S., M.-L. Gr a i c h e n , A. Bö r n e r & U. Lo h w a s s e r : Zwölf tolerance in barley (Hordeum vulgare) could be used for Institutionen aus zehn Ländern an Europa-Projekt marker-assisted selection. In: Ve i s z , O. (Ed.): Agrisafe Final zur Blattgemüse-Züchtung beteiligt ‑ Netzwerk für Conference “Climate change: Challenges and opportunities Resistenzzüchtung bei Salaten im Aufbau. Gemüse 8 (2010) in agriculture” (21.-23.03.2011, Budapest/Hungary). 20-22. Agricultural Research Institute of the Hungarian Academy of Ul r i c h , D., H. Bu d a h n , T. St r u c k m e y e r , F. Ma r t h e , H. Kr ü g e r & U. Sciences (2011) 215-217. Lo h w a s s e r : Diversity of volatile patterns in a genebank collection of parsley (Petroselinum crispum [Mill.] Nyman). Proceedings of the 12th International Weurman Flavour Other Papers Research Symposium “Expression of Multidisciplinary Flavour Science”, Juli 2008, Interlaken, Switzerland (2010) 2010 383-386. Bö r n e r , A., A.K. Jo s h i , E.K. Kh l e s t k i n a , B. Ko b i l j s k i , I. Kr a n n e r , U. Ku m a r , Ya n g , Q., A. Bö r n e r & M. Kr u s e : Bestimmung der optimalen S. La n d j e v a , L.N. Le o n o v a , U. Lo h w a s s e r , M. Na g e l , S. Na v a k o d e , K. Lagerungsbedingungen für die ultra-dry Saatgutlagerung Ne u m a n n , R. Pa l i w a l , M.A. Re h m a n Ar i f , M.S. Rö d e r , N. Ti k h e n k o , A. von Zwiebel (Allium cepa L.), Weizen (Triticum aestivum L.) We i d n e r & K. Za y n a l i Ne z h a d : Items from Germany. Ann. Wheat und Raps (Brassica napus L.). Berichte der Gesellschaft für Newsl. 56 (2010) 47-52. Pflanzenbauwissenschaften, Bd. 5 (2010) 16-19. Bö r n e r , A., S. La n d j e v a , K.F.M. Sa l e m & U. Lo h w a s s e r : Plant genetic resources – a prerequisite for drought tolerance breeding in 2011 cereals. Tagungsband der 60. Jahrestagung der Vereinigung Bö r n e r , A., E.V. An t o n o v a , H.J. K, E.K. Kh l e s t k i n a , B. Ko b i l j s k i , S. der Pflanzenzüchter und Saatgutkaufleute Österreichs 2009 Ko l l e r s , U. Lo h w a s s e r , M. Na g e l , K. Ne u m a n n , M.A. Re h m a n -Ar i f , N. (2010) 11-13. Ti k h e n k o , K. Za y n a l i Ne z h a d & M.S. Rö d e r : Items from Germany. Bö r n e r , M., M. Na g e l , K. Ne u m a n n , U. Lo h w a s s e r , K. Fö r s t e r & A. Ann. Wheat Newsl. 57 (2011) 12-15. Bö r n e r : Assoziationsgenetische Pilotstudie zur Langlebigkeit von Gerste (Hordeum vulgare L.). Berichte der Gesellschaft für Pflanzenbauwissenschaften, Bd. 5 (2010) 101-103.

41 Abteilung Genbank/Department of Genebank

Kik, C., R. v a n Tr e u r e n , D. Pi n k , D. As t l e y , P. Co q u i n , V. Ca d o t , A. Ro s e n h a u e r , M.: Genetische Untersuchungen des Merkmals Bö r n e r , U. Lo h w a s s e r , S. Th u m m , A. Le b e d a , E. Kr i s t k o v a , S. So l b e r g , Langlebigkeit von Saatgut an der Spezies Brassica napus K. An t o n i u s , B. Ma i s o n n e u v e , F. Le r c h , P. Su m p t i o n , F. D’An t u o n o , L. (Diploma Thesis) Hochschule für Technik und Wirtschaft, V. Me g l i c & B. Ba r t h a : Leafy vegetables GENRES project Fachbereich Landbau/Landespflege, Dresden (2010) 126 successfully completed! Newsl. Europe 42 (2011) 12. pp. Lo h w a s s e r , U. & A. Bö r n e r : Saatgut im Wandel der Zeit. Getreide Za y n a l i Ne z h a d , K.: Genetic linkage map construction and Magazin 4 (2011) 66. identification of Quantitative Trait Loci (QTLs) determining postanthesis drought tolerance and other agronomic traits in bread wheat. (PhD Thesis) Martin-Luther-Universität Electronic Publications Halle-Wittenberg, Naturwissenschaftliche Fakultät, Halle/S. 2010 (2010) 267 pp. Op p e r m a n n , M., J. Ke i l w a g e n , H. Kn ü p f f e r , S. Fr i e d e l & A. Bö r n e r : Validation, analysis and aggregation of long-term trait 2011 observation data of genebank material. In: We i t z m a n n , Na g e l , M.: Seed survival in genebanks ‑ genetic and biochemical A.L. (Ed.), Proceedings of TDWG (2010). Woods Hole, aspects of seed deterioration in barley. (PhD Thesis) Georg- Massachusetts, USA (2010) 52-53. http://www.vliz.be/ August-Universität, Göttingen (2011) 121 pp. imisdocs/publications/215525.pdf (2010). Ne u m a n n , K.: Genomweite Assoziationsstudie zur Identifizierung von Loci für juvenile und adulte Trockentoleranz in Gerste. (PhD Thesis) Martin-Luther-Universität Halle-Wittenberg, PhD and Diploma Theses Halle/S. (2011) 113 pp. 2010 Bö r n e r , M.: Langlebigkeit von Saatgut: Assoziationsgenetische Studie anhand einer künstlich gealterten Kollektion der Additional Publications 2009 Gerste (Hordeum vulgare L.). (Bachelor) Martin-Luther- As c h , F. & A. We i d n e r : Klimawandel und Pflanzenzüchtung: Eine Universität Halle-Wittenberg, Halle/S. (2010) 58 pp. transdisziplinäre Herausforderung. Vortr. Pflanzenzücht. 81 Br a t h u h n , A.: Blütenbiologische Untersuchungen an Kulturen (2009) 25-40. im Freiland und in Kleingewächshäusern der Genbank des IPK Gatersleben. (Bachelor) Fachhochschule Wiesbaden, Standort Geisenheim (2010) 40 pp.

42 Research Group: In vitro Storage and Cryopreservation Head: Dr. Joachim Keller

Scientists Based on results of previous measurements by Differential Scanning Calorimetry, the cryopreservation protocol of IPK financed shallots was modified by omission of preculture and shorter Zanke, Christine, Dr. (01.04.-31.05.2011) pretreatments in cryoprotectant solutions. Two methods were compared, namely droplet vitrification and encapsulation Grant Positions vitrification.T he latter did not lead to improved results and was Zanke, Christine, Dr. (EU, till 31.03.2011) not followed further.

Visiting Scientists/Scholars Pollen cryopreservation was successfully applied in the Olas-Sochacka, Marta (COST Action 871, 19.10.-18.11.2010) two model species Allium cepa and A. obliquum. In the latter Pelc, Malgorzata, Dr. (COST Action 871, 07.04.-28.05.2010) species, seed set after fertilisation with cryopreserved pollen was successfully tested (A. Senula, D. Büchner, J. Keller).

Goals The project EURALLIVEG funded by the European Commission DG Agriculture under the Council Regulation No 870/2004 In vitro maintenance of vegetatively propagated genebank was finalised. Within this project a documentation of a garlic accessions, cryopreservation of potato, garlic, and mint. and shallot core collection was completed including the Research on tissue water conditions and cold adaptation regeneration rates of the cryopreserved garlic accessions. connected with influence of ultra-low temperatures on plant The Tripartite Garlic Cryobank was established including organs. Integration of new cryopreservation strategies, such as safety duplication between the three partner genebanks pollen storage, into germplasm management. of Czech Republic, Germany and Poland. AFLP analysis was done on 219 garlic and 63 shallot accessions. SNP analysis was performed in the laboratory of the company Trait Genetics Research Report on 24 garlic accessions. The results were used to develop new markers. A total of 220 accessions, 67 of them from IPK, Accessions of the genera Allium, Antirrhinum, Artemisia, were completely cryopreserved consisting either of 100 Brassica, Dioscorea, Mentha, Orthosiphon, and Sechium are explants with regeneration rates better than 30 % or of 200 in in vitro maintenance in total comprising 368 clones of 339 explants with regeneration rates between 10 and 30 %. Virus genebank accessions. For distribution to the users 96 samples elimination, performed in the frame of EURALLIVEG was of mint and yams accessions were provided (M. Grübe, D. supported by training courses and plant material. The project Büchner, A. Senula). website is permanently updated and will be continuously The cryo-collection of potato was increased to 1,289 clones. updated (C. Zanke, J. Keller, F. Blattner, A. Senula). Within the For distribution to the users 10 samples were provided to AEGIS strategy of the European Cooperative Programme for the Satellite Collections North (M. Grübe). Plant Genetic Resources (ECPGR), a project was completed on cryopreservation of young inflorescence bases in bolting The Allium collection established in cryopreservation garlic for germplasm storage. This system has the advantage amounts to 101 accessions, amongst them 96 of garlic, 2 that the material is cleaner than bulbs, the procedure is shorter of Allium hybrids and 3 of A. obliquum. The transfer of virus- than use of in vitro donor material and the combination of this free garlic material into protected greenhouse and field method used in summer with bulbil cryopreservation in winter conditions was continued. Virus-free material was provided to increases cryopreservation efficiencyC ( . Zanke, J. Keller). In the cryopreservation routine (A. Senula, D. Büchner, J. Keller). the frame of the EU-funded COST Action 871 two Short Term Scientific Missions were organised and supervised. First tests The droplet vitrification method was further used routinely on the influence of ascorbic acid as antioxidant were done on for cold-hardened plants of mint. Seventy-one accessions mint and garlic (A. Senula, C. Zanke, M. Pelc, M. Olas-Sochacka). from 15 species are kept in cryopreservation with 200 explants Furthermore, the influence of abscisic acid has been tested each. Regeneration rates varied between 30 and 98 %. The on mint cryopreservation (A. Senula, M. Muminova). A study cryopreservation method has been optimised by using donor on the costs of garlic cryopreservation was completed. Data in vitro plantlets directly from medium-term cold storage at on various field culture and cryopreservation strategies were 10 °C. This results in a shorter duration of the preparative phase compared and efficiency aspects were discussedA ( . Breuing, prior to introduction into liquid nitrogen and does not allow see Fig. 17, p. 44). accumulation of endophytes. As a result, better regeneration rates were achieved (A. Senula).

43 Abteilung Genbank/Department of Genebank

Fig. 17 Overview on activities for cryopreservation and virus elimination. Diagram: Distribution of taxa in the EURALLIVEG collection of 263 accessions. Right side: target plants of EURALLIVEG garlic (above) and shallot (below). Lower row from left to right: Donor organs for the AEGIS project on unripe inflorescences and regeneration from inflorescence explants 14 days after rewarming, meristem explants of garlic as needed to be used for virus elimination and regeneration stage in shallot experiments after pretreaments with cryoprotectants without cryopreservation (Photos: J. Keller, A. Senula, C. Zanke).

Publications Za n k e , C., E.R.J. Ke l l e r , T. Ko t l i ń s k a , M. Ol a s & J. Zá m e č n í k : Cryopreservation of vegetative garlic for the establishment Peer Reviewed Papers of a European core collection. 1st Int. Symp. of ISHS Cryopreservation in Horticultural Species, Leuven/Belgium, 2010 5-8 April 2009. Acta Horticult. 908 (2011) 431-438. Ka c z m a r c z y k , A., A. Ho u b e n , E.R.J. Ke l l e r & M.F. Me t t e : Influence of cryopreservation on the epigenetic state of potato. CryoLetters 31 (2010) 380-391. Books and Book Chapters Ly n c h , P., G. So u c h , S. Tr i g w e l l , E.R.J. Ke l l e r & K. Ha r d i n g : Plant Cryopreservation: From laboratory to genebank. Asia Pac. J. 2010 Mol. Biol. Biotechnol. 18 (2010) 239-242. Ke l l e r , E.R.J. & A. Se n u l a : Cryopreservation of plant germplasm. In: Da v e y , M.R. & P. An t h o n y (Eds.): Plant Cell Culture: Essential 2011 Methods. Wiley (2010) 131-152. Ka c z m a r c z y k , A., V.-M. Ro k k a & E.R.J. Ke l l e r : Potato shoot tip cryopreservation, a review. Potato Res. 54 (2011) 45-79. 2011 Ka c z m a r c z y k , A., C. Za n k e , A. Se n u l a , M. Gr ü b e & E.R.J. Ke l l e r : Ke l l e r , E.R.J., D. As t l e y , A. Ts i v e l i k a s , J. En g e l s & E. Li p m a n (Eds.): Report Thermal analyses by differential scanning calorimetry for of a Working Group on Allium. 7th Meeting, 6-8 September cryopreservation of potato shoot tips. 1st Int. Symp. of ISHS 2011, Perea, Thessaloniki/Greece. Bioversity International, Cryopreservation in Horticultural Species, Leuven/Belgium, Rome, Italy (2011) 32 pp. 5-8 April 2009. Acta Horticult. 908 (2011) 39-46. Ke l l e r , E.R.J., A. Se n u l a & C. Za n k e : Alliaceae in cryopreservation, achievements and constraints. 1st Int. Symp. of ISHS Other Papers Cryopreservation in Horticultural Species, Leuven/Belgium, 2010 5-8 April 2009. Acta Horticult. 908 (2011) 495-508. Ke l l e r , E.R.J.: COST Action “CRYOPLANET” meets Society of Low Se n u l a , A. & E.R.J. Ke l l e r : Cryopreservation of mint – routine Temperature Biology in Germany. Biovers. Newsl. Europe 40 application in a genebank, experience and problems. 1st (2010) 15. Int. Symp. of ISHS Cryopreservation in Horticultural Species, Ma g g i o n i , L., M.C. Da u n a y , W. v a n Do o i j e w e e r t , D. As t l e y , N. Ba s , F. Leuven/Belgium, 5-8 April 2009. Acta Horticult. 908 (2011) Br a n c a , M.J. Dí e z Ni c l ó s , E. Ge o f f r i a u , E.R.J. Ke l l e r , T. Ko t l i ń s k a , K. 467-475. Sm é k a l o v á , R. v a n Tr e u r e n & E. Li p m a n (Eds.): Report of a Vegetables Network, 3rd Meeting, 10-12 November 2009, Catania/Italy. Bioversity International, Rome, Italy (2010) 59 pp.

44 2011 PhD and Diploma Theses Ke l l e r , E.R.J., C.D. Za n k e , T. Ko t l i n s k a , M. Ol a s -So c h a c k a , A. Re i s & A.M. 2010 Ba r a t a : Broadening the spectrum of usable organ sources for Br e u i n g , A.: Einschätzung der Kosteneffektivität verschiedener cryopreservation of garlic – An AEGIS project report. Biovers. Strategien der Langzeiterhaltung von Knoblauch (Allium sa- Newsl. Europe 53 (2011) 9. tivum L.) in der Genbank Gatersleben. (Master Thesis) Leib- niz-Universität Hannover, Naturwissenschaftliche Fakultät, Institut für Zierpflanzen- und Gehölzwissenschaften, Han- Electronic Publications nover (2010) 186 pp. 2010 Co l m s e e , C., C. Za n k e , T. Fu n k e , S. We i s e , E.R.J. Ke l l e r & U. Sc h o l z : The Garlic and Shallot Core Collection (GSCC). http://www.ipk- Additional Publications 2009 gatersleben.de/databases/genetic_resources/gscc (2010). As t l e y , D. & E.R.J. Ke l l e r : Working Group on Allium. In: As t l e y , Ke l l e r , E.R.J. & T. Fu n k e : Die Knoblauch-Kernkollektion des D., N. Ba s , F. Br a n c a , M.C. Da u n a y , M.J. Di e z , E.R.J. Ke l l e r , W. v a n IPK - IPK’s Core Collection of Garlic. http://pgrc-35.ipk- Do o i j e w e e r t , R. v a n Tr e u r e n , L. Ma g g i o n i & E. Li p m a n (Eds.): Report gatersleben.de/apps/gcc/index.htm (2010). of a Vegetables Network. 2nd Meeting, June 2007, Olomouc/ Ke l l e r , E.R.J., C. Za n k e & U. Sc h o l z : EURALLIVEG ‑ Vegetative Allium, Czech Republic. Bioversity International, Rome/Italy (2009) Europe’s Core Collection, Safe and Sound http://euralliveg. 8-13. ipk-gatersleben.de (2010).

2011 Co l m s e e , C., C. Za n k e , T. Fu n k e , S. We i s e , E.R.J. Ke l l e r & U. Sc h o l z : The Garlic and Shallot Core Collection (GSCC). http://www.ipk- gatersleben.de/databases/genetic_resources/gscc (2011). Ke l l e r , E.R.J. & T. Fu n k e : Die Knoblauch-Kernkollektion des IPK - IPK’s Core Collection of Garlic. http://pgrc-35.ipk- gatersleben.de/apps/gcc/index.htm (2011). Ke l l e r , E.R.J., C. Za n k e & U. Sc h o l z : EURALLIVEG - Vegetative Allium, Europe’s Core Collection, Safe and Sound http://euralliveg. ipk-gatersleben.de (2011).

45 Abteilung Genbank/Department of Genebank

Research Group: Satellite Collections North Head: Dr. Klaus J. Dehmer

Scientists Julius Kühn-Institute (JKI), Institute for Breeding Research on Agricultural Crops) and tubers (150 WKS accessions/885 IPK financed genotypes; JKI) and Synchytrium endobioticum (10 AKS, 36 KKS Nehrlich, Stephanie (0.50, since 01.10.2010) accessions; JKI/Institute for Plant Protection in Field Crops and Willner, Evelin (till 30.09.2010; 0.50, since 01.10.2010) Grassland). For all pests, complete resistance was observed in several instances, for WKS entries both on the genotype and even Grant Positions on the accession level. Tubers of 94 entries (16 AKS; 78 KKS) were Diekmann, Kerstin, Dr. (BMELV, since 01.02.2010) cultivated for the evaluation of their taste, while starch content Gernand-Kliefoth, Dorota, Dr. (BMELV, till 30.04.2011) was determined in tubers of 161 accessions. For a project on the Gerson, Lydia (0.50 Bayerische Landesanstalt für Landwirtschaft) evaluation of potato tuber quality, tubers produced from 80 Schlichting, Andre, Dr. (0.25 BMELV, 01.01.-30.04.2010) KKS accessions, 77 AKS accessions and 42 WKS genotypes were Witter, Steffi (0.50 BMELV/FNR, since 01.11.2010) evaluated at the University of Rostock and at Steinbeis Transfer Center (STZ) Soil Biotechnology, and a large variability was detected for most traits investigated (A. Schlichting). Research on Goals degree of diversity and duplication within the clonal collections, as well as on genetic stability under different regeneration Collection, conservation, evaluation, distribution and research re- regimes and wild species taxonomy was conducted using retro garding plant genetic resources of potatoes, oil and fodder crops. transposon-based markers (K. Diekmann). In potato, these proved equally informative as the well established SSR markers, even with lower numbers of markers applied. Research Report The Malchow/Poel Oil Plants and Fodder Crops Collections The Groß Lüsewitz Potato Collections (GLKS; K.J. Dehmer) (E. Willner, S. Nehrlich) consist of 10,441 samples of (fodder) include a total of 6,124 accessions from 140 tuber bearing grasses, 2,472 oil plants and 1,345 forage legumes (total 14,258 Solanum species within three separate collections. They accessions). comprise 2,849 accessions of 139 wild and cultivated species from Southern and Central America (WKS, sexually propagated), In 2010 and 2011, a total of 2,780 (1,093; 1,687) accessions were 2,736 Solanum tuberosum ssp. tuberosum cultivars, landraces cultivated, either for multiplication and characterisation (1,779) or breeding lines (KKS, vegetatively propagated) and clonally or evaluation (1,001; Fig. 18, p. 47). Germination tests were propagated genotypes of Andean or equatorial origin (AKS; conducted for 12,670 accessions (2010: 6,506; 2011: 6,164). 539 entries, mainly seven cultivated species). According to FAO genebank standards, 76 % of the whole Malchow collection is stored as an active and base collection In 2010 and 2011, 292 WKS accessions (152; 140) were multiplied with safety duplicates at the Svalbard Global Seed Vault (9,248 in the greenhouse, while a total of 171 AKS (79; 92) and 1,287 KKS accessions, 64 % of total Malchow stocks), while 82 % of the accessions (617; 670) were cultivated in the field with 10 plants whole collection is available for seed requests. A total of 3,666 each and characterised for 15 traits. Via in vitro culture with a samples (1,325; 2,341) were provided to 228 users (88; 140). microtuber storage step, 2,361 KKS and 539 AKS accessions Characterisations for 15 traits were performed on 1,947 grass are maintained. More than 1,250 KKS accessions are kept as accessions (620; 1,327), 275 crucifers (121; 154) and 301 legumes cryopreserved safety duplicates at IPK Gatersleben (research (123; 178) for an initial description of their morphological and group In vitro Storage and Cryopreservation). Germination phenotypical traits as well as for the confirmation of their rates were determined for 1,600 WKS entries. 3,890 potato botanical classification. Field evaluations were carried out accessions (2010: 1,925; 2011: 1,965) were distributed in the for Lolium perenne (46 accessions as recently bred material in context of 523 requests (222; 301). cooperation with Agricultural Research Institute Mecklenburg, West Pomerania), which were compared to standard varieties According to the respective ELISA tests, 2,744 in vitro samples in a three-year trial for trait variability and green matter yield. (95 %) are free of the six most common potato viruses. The State Plant Protection Offices at Hannover and Rostock tested The European Central Poa Database (http://poa.ipk- potato accessions of the GLKS for quarantine viruses (293 accs.), gatersleben.de) was developed further. Progress was made quarantine bacteria (945), and PSTVd (2,144). in the identification ofO “ riginality” (98 % of the entries) and Evaluations were carried out for resistance to Globodera pallida “Primary Holder” (86 % of the accessions) matters, which will (174 WKS accessions/804 genotypes, State Plant Protection Office allow to avoid unnecessary regenerations and to improve the Rostock), Phytophthora infestans on leaves (299 WKS accessions; sharing of responsibilities.

46 Fig. 18 Multi-purpose building and greenhouse of IPKs Fodder and Oil Crops Collections at Malchow (Photo left, S. Nehrlich) and multiplication of accessions of different grass genera in an isolation plot on Poel island (Photo right, K. Ploen).

Genebank and breeding material from the three grass genera Ne s t m a n n , S., T. Sr e t e n o v i c Rajicic, K.J. De h m e r , M. Fi s c h e r , J. Dactylis, Lolium and Phleum was examined in diversity and Sc h u m a c h e r & C. Ro s c h e r : Plant species diversity and introgression studies (D. Gernand-Kliefoth). Here, SSR markers composition of experimental grasslands affect genetic facilitated the identification of interspecific hybrids, whileI G SH differentiation of Lolium perenne populations. Mol. Ecol. 20 experiments proved the presence of introgressed donor chromatin (2011) 2188-2203. even after several backcross generations. In cooperation with an Wa l t e r , J., L. Na g y , R. He i n , U. Ra s c h e r , C. Beierkuhnlein , E. Wi l l n e r & A. external partner (Bavarian State Research Center for Agriculture), Je n t s c h : Do plants remember drought? Hints towards a drought- the complex trait ‘persistence’ was analysed in Lolium perenne memory in grasses. Environ. Exp. Bot. 71 (2011) 34-40. (L. Gerson), while the elucidation of heterotic groups for biomass production in Lolium hybrids (S. Witter) and drought tolerance in Lolium were in the scope of two further research projects. Books and Book Chapters

2010 Publications Wi l l n e r, E., S. Hü n m ö r d e r & K.J. De h m e r : Towards an enhanced utilization of plant genetic resources in grass breeding by Peer Reviewed Papers characterization and evaluation trials. In: Hu y g h e , C. (Ed.): Sustainable Use of Genetic Diversity in Forage and Turf 2010 Breeding. Springer, The Netherlands (2010) 173-180. Mi c h a l s k i , S.G., W. Du r k a , A. Je n t s c h , J. Kr e y l i n g , S. Po m p e , O. Sc h w e i g e r , E. Wi l l n e r & C. Beierkuhnlein : Evidence for genetic differentiation and divergent selection in an autotetraploid Other Papers forage grass (Arrhenatherum elatius). Theor. Appl. Genet. 120 2010 (2010) 1151-1162. Ro s e n h a u e r , M., M. Na g e l , E. Wi l l n e r, F.-G. Sc h r ö d e r , R. Sn o w d o n , W. Sc h u b i g e r , F.X., J. Ba e r t , B. Ba y l e , P. Bo u r d o n , B. Ca g a s , V. Ce r n o c h , Fr i e d t & A. Bö r n e r : Genetische Untersuchungen des Merkmals E. Cz e m b o r , F. Ei c k m e y e r , U. Fe u e r s t e i n , S. Ha r t m a n n , H. Ja k e s o v a , Langlebigkeit von Saatgut der Spezies Brassica napus L. D. Jo h n s t o n , B. Kr a u t z e r , H. Le e n h e e r , H. Le l l b a c h , C. Pe r s s o n , Berichte der Gesellschaft für Pflanzenbauwissenschaften, W. Pi e t r a s z e k , U.K. Po s s e l t , M. Ro m a n i , L. Ru s s i , S. Sc h u l z e , M.C. Bd. 5 (2010) 108-111. Ta r d i n , F. Va n He e , L. v a n Kr u i j s s e n , P. Wilkins, E. Wi l l n e r, L. Wo l t e r s & B. Bo l l e r : Susceptibility of European cultivars of Italian and 2011 perennial ryegrass to crown and stem rust. Euphytica 176 Sc h l i c h t i n g , A., P. Le i n w e b e r & K.J. De h m e r : Innovative Analysen (2010) 167-181. von Inhaltsstoffen und Qualitätsparametern. Kartoffelbau 9 v a n d e Wi e l , C.C.M., T. Sr e t e n o v i c Rajicic, R. v a n Tr e u r e n , K.J. De h m e r , (2011) 46-47. C.G. v a n d e r Li n d e n & T.J.L. v a n Hi n t u m : Distribution of genetic diversity in wild European populations of prickly lettuce (Lactuca serriola): implications for plant genetic resources Electronic Publications management. Plant Genet. Resour. 8 (2010) 171-181. 2010 2011 Ma g g i o n i , L., M. Ve t e l ä i n e n , E. Wi l l n e r & E. Li p m a n : Report of a Beierkuhnlein , C., D. Th i e l , A. Je n t s c h , E. Wi l l n e r & J. Kr e y l i n g : Ecotypes Working Group on Forages. Tenth Meeting, 28-29 April 2010, of European grass species respond differently to warming Poel Island, Germany. http://www.ecpgr.cgiar.org/networks/ and extreme drought. J. Ecol. 99 (2011) 703-713. forages/forages_wg_germany_2010.html (2010).

47 Abteilung Genbank/Department of Genebank

Programme: Taxonomy and Evolution Research Group: Experimental Taxonomy Head: Dr. Frank Blattner

Scientists pilot study on polyploid evolution in wall barley (H. murinum) we could show that two extinct diploid taxa together with IPK financed subsp. glaucum were involved in the formation of extant tetra- Baier, Christina, (0.50, till 31.08.2010) and hexaploid subspecies of this taxon. Similar analyses are Benor, Solomon (0.50, 01.10.-26.11.2011) currently conducted on all polyploid species of Hordeum within Ekhvaia, Jana (0.50, 01.09.-30.11.2010) a DFG-funded project. Also through DFG funding a study on Gurushidze, Maia, Dr. (0.50, 01.04.-30.09.2011) the influence of flowering time differences on species stability Jakob, Sabine, Dr. (till 31.12.2011) in sympatric geographical settings in Patagonia could be Nürk, Nicolai Matthias (0.50, 01.09.2010-31.08.2011) started (within the DFG Priority Programme on flowering time) and analyses of Central Asian species of the genus continued Grant Positions (S. Jakob, J. Brassac, K. Herrmann). Harpke, Dörte, Dr. (0.50 DFG, since 01.05.2010) Herrmann, Katja (0.65 DFG, since 01.08.2011) In the DFG-funded project on Hypericum systematics we Bernhardt, Nadine (0.65 DFG, since 01.06.2011) finished analyses of molecular and morphological characters Brassac, Jonathan (0.65 DFG, since 01.02.2010) including more than 450 species of the genus plus representatives Nürk, Nicolai Matthias (DFG, till 31.08.2010) of other genera of the family Hypericaceae. Through this analysis we were able to evaluate earlier theories of character Visiting Scientists/Scholars evolution, geographic origin and taxonomic treatment of the Bachmann, Konrad, Prof. (self-financed, till 31.05.2011) genus. We could show that Hypericum originated during the Baier, Christina (self-financed, 01.09.2010-31.03.2011; Miocene in Laurasia from tropical progenitors, when cooling 01.07.-31.12.2011) climate conditions resulted in expanding temperate habitats. Benor, Solomon (DAAD, till 30.09.2011) Apomictic species evolved independently in at least three different clades of the genus (see Fig. 19, p. 49). The project is part of cooperation with the Natural History Museum London, Goals and connected to projects on Hypericum in the groups of T. Sharbel and H. Bäumlein at the IPK and M. Koch’s group at the Phylogenetic classification and analysis of evolution of University of Heidelberg (N. Nürk). crops and their wild relatives. Experimental studies to link molecular markers and phylogenetic data with ecological Species of the Euphorbiaceae genus Macaranga are important and morphological traits, and to analyse plant–environment Southeast Asian pioneer shrubs and trees of areas where the interdependency on and below the species level in an rainforest was freshly logged. Many of these species co-occur evolutionary framework. with mutualistic ants. In cooperation with the universities in Kassel and Würzburg we study speciation processes, probably driven by co-evolution between plants and their ant partners. Research Report In population genetic and phylogeographic analyses of two widespread Bornean Macaranga species, one a myrmecophyte The major aim of the group is to understand mechanisms (ant-plant), the other without ant mutualists, we studied the resulting in speciation processes in specific plant groups.T his influence of mutualistic ants on dispersal abilities of their involves the study of the distribution of species, populations host plant species. We found clear differences between the and genotypes in time and space together with the analysis of genepools on the Malayan Peninsula and Borneo, and also character state changes involved in environmental adaptation within Borneo a separation of populations in the northeast from and reproductive isolation. These characters (e.g., abiotic genotypes occurring in the southwest of the island, indicating stress tolerance, flowering time) influence the ecological niches partly long-term isolation of these areas. In comparison to of organisms and are often also important agronomic traits. the non-myrmecophyte, populations of the myrmecophytic species were genetically much more differentiated, indicating In the barley genus Hordeum we analyse all aspects related restricted gene flow in this species. This probably results to phylogeny, speciation and ecological adaptation. During from the need of the plant to co-disperse with its ant partner, the last two years we analysed the evolution of ecological as seedlings not readily colonised by the ants will soon die niches in the diploid South American Hordeum species and (C. Baier). combined these data with population genetic approaches. We inferred that population demography influences the ability of species to change or maintain their ecological niches. In a

48 Fig. 19 Bayesian tree from the analysis of nrDNA ITS sequence data, showing relationships of 194 Hypericum and 12 outgroup species, represented by 366 accessions. A light-grey background highlights clades of Hypericum, dark-grey other taxa of Hypericaceae. Asterisks indicate maximum statistical support for branches. Occurrence of apomixis (APO) is marked on the tree. Evolution of habit, shrubs (in green), trees (red), perennial (dark blue) and annual herbs (light blue), is depicted by colour of branches. The four pie charts display the proportion of character states reconstructed over 1000 trees at a certain node (N. Nürk).

49 Abteilung Genbank/Department of Genebank

Several species of the jute genus Corchorus (Malvaceae s.l.) Publications are used in African countries as vegetables or salad in addition to their properties as fibre plants. These species are so-called Peer Reviewed Papers neglected crops. Ethnobotanical data on the Ethiopian species were collected and detailed molecular and morphological 2010 studies of C. olitorius were conducted. The analyses prove Be n o r , S., F.R. Bl a t t n e r , S. De m i s s e w & K. Ha m m e r : Collection and an African origin of cultivated jute and provide evidence ethnobotanical investigation of Corchorus species in for Ethiopia as an important genotype reservoir for jute Ethiopia: potential leafy vegetables for dry regions. Genet. breeding. Phylogenetic analysis of the genus inferred several Resour. Crop Evol. 57 (2010) 293-306. intercontinental long-distance dispersals out of Africa to Fr i t s c h , R.M., F.R. Bl a t t n e r & M. Gu r u s h i d z e : New classification Australasia and South America (S. Benor). of Allium L. subg. Melanocrommyum (Webb & Berthel.) Rouy (Alliaceae) based on molecular and morphological The genus Crocus consists of about 80 species distributed characters. Phyton 49 (2010) 145-220. from Western Europe to the western-most parts of China. Many Gu r u s h i d z e , M., R.M. Fr i t s c h & F.R. Bl a t t n e r : Species-level phylogeny Crocus species are used as ornamentals. In a DFG-funded project of Allium subgenus Melanocrommyum: Incomplete lineage we constructed a phylogeny of the genus based on two nuclear sorting, hybridization and trnF gene duplication. Taxon 59 marker regions. We were able to resolve species relationships, (2010) 829-840. trace the evolution of chromosome numbers in the genus, and Ja k o b , S.S. & F.R. Bl a t t n e r : Two extinct diploid progenitors were could show that many of the different subspecies of C. biflorus involved in allopolyploid formation in the Hordeum murinum occurring on mountaintops in Anatolia merit species rank. (Poaceae: Triticeae) taxon complex. Mol. Phylogenet. Evol. We now use these results as basis for phylogeographic and 55 (2010) 650-659. population genetic analyses of the Anatolian C. biflorus taxon Ja k o b , S.S., C. He i b l , D. Ro d d e r & F.R. Bl a t t n e r : Population complex to infer modes of speciation on sky islands in this area demography influences climatic niche evolution: evidence (D. Harpke). from diploid American Hordeum species (Poaceae). Mol. Ecol. 19 (2010) 1423-1438. Recently a project aiming at clarifying the phylogeny of Ko t s e r u b a , V., K. Pistrick, F.R. Bl a t t n e r , K. Ku m k e , O. We i s s , T. Ru t t e n , Triticeae was approved by DFG. Although relationships in this J. Fu c h s , T. En d o , S. Na s u d a , A. Gh u k a s y a n & A. Ho u b e n : The important group of grasses were analysed for nearly a century, evolution of the hexaploid grass Zingeria kochii (Mez) Tzvel. up to now no conclusive hypothesis could be reached. This (2n=12) was accompanied by complex hybridization and might partly be due to occasional gene flow among species uniparental loss of ribosomal DNA. Mol. Phylogenet. Evol. 56 belonging even to different genera. In this project we will (2010) 146-155. use next generation sequencing to compare gene regions in Ma r c h e l l i , P., C. Ba i e r , C. Me n g e l , B. Zi e g e n h a g e n & L. Ga l l o : a genome-wide approach among all diploid species of the Biogeographic history of the threatened species Araucaria tribe to understand reasons for inconsistencies among the araucana (Molina) K. Koch and implications for conservation published studies and to arrive at a comprehensive phylogeny – a case study with organelle DNA markers. Conserv. Genet. for the group (N. Bernhardt). 11 (2010) 951-963. Nü r k , N.M. & F.R. Bl a t t n e r : Cladistic analysis of morphological characters in Hypericum (Hypericaceae). Taxon 59 (2010) 1495-1507. Pe t e r s o n , A., D. Ha r p k e , L. Pe r u z z i , J.-M. Ti s o n , H. Jo h n & J. Pe t e r s o n : Gagea bohemica (Liliaceae), a highly variable monotypic species within Gagea. Plant Biosystems 144 (2010) 308-322. Sc h a l l a u , A., F. Ar z e n t o n , A.J. Jo h n s t o n , U. Hä h n e l , D. Ko s z e g i , F. Bl a t t n e r , L. Al t s c h m i e d , G. Ha b e r e r , G. Ba r c a c c i a & H. Bä u m l e i n : Identification and genetic analysis of the APOSPORY locus in Hypericum perforatum L. Plant J. 62 (2010) 773-784. We i g e n d , M., M. Go t t s c h l i n g , H.H. Hi l g e r & N.M. Nü r k : Five new species of Lithospermum L. (Boraginaceae tribe Lithospermeae) in Andean South America – another radiation in the Amotape- Huancabamba Zone. Taxon 59 (2010) 1161-1179.

50 2011 Books and Book Chapters Be n o r , S., J. Fu c h s & F.R. Bl a t t n e r : Genome size variation in Corchorus olitorius (Malvaceae s.l.) and its correlation with 2010 elevation and phenotypic traits. Genome 54 (2011) 575- Bl a t t n e r , F.R., T. Pl e i n e s & S.S. Ja k o b : Rapid radiation in the barley 585. genus Hordeum (Poaceae) during the pleistocene in the Bo r d b a r , F., M.R. Ra h i m i n e j a d , H. Sa e i d i & F.R. Bl a t t n e r : Phylogeny Americas. In: Gl a u b r e c h t , M. (Ed.): Evolution in Action. and genetic diversity of D-genome species of Aegilops Springer, Heidelberg (2010) 17-34. and Triticum (Triticeae, Poaceae) from Iran based on We i s i n g , K., D. Guicking, C. Fe y -Wa g n e r , T. Kr ö g e r -Kilian, T. Wö h r m a n n , microsatellites, ITS and trnL-F. Plant Syst. Evol. 291 (2011) W. Do r s t e w i t z , G. Bä n f e r , U. Mo o g , M. Vo g e l , C. Ba i e r , F.R. Bl a t t n e r , 117-131. H. Fe l d h a a r & B. Fi a l a : Mechanisms of speciation in Southeast Guicking, D., B. Fi a l a , F.R. Bl a t t n e r , F. Sl i k , M. Mo h a m e d & K. We i s i n g : Asian ant-plants of the genus Macaranga (Euphorbicaceae). Comparative chloroplast DNA phylogeography of two In: Gl a u b r e c h t , M. (Ed.): Evolution in Action. Springer, tropical pioneer trees, Macranga gigantea and M. pearsonii Heidelberg (2010) 169-191. (Euphorbiaceae). Tree Genet. Genomes 7 (2011) 573-585. Ka r i m i As h t i y a n i , R., A.M. Ba n a e i Mo g h a d d a m , V. Sc h u b e r t , T. Ru t t e n , J. Fu c h s , D. De m i d o v , F.R. Bl a t t n e r & A. Ho u b e n : AtHaspin Other Papers phosphorylates histone H3 at threonine 3 during mitosis 2011 and contributes to embryonic patterning in Arabidopsis. Ha r p k e , D. & A. Pe t e r s o n : Molekulare Systematik und Taxonomie Plant J. 68 (2011) 443-454. in der Gattung Mammillaria. Mitteilungsblatt Arbeitskreis Nü r k , N.M. & S.L. Cr o c k e t t : Morphological and phytochemical für Mammillarienfreunde 1 (2011) 2-11. diversity among Hypericum species of the Mediterranean Nü r k , N.M.: Johanniskraut – von seiner Wirkung zum Namen basin. Med. Aromat. Plant Sci. Biotechnol. 5 (2011) 14-28. und zur Botanik. Aromareport 5 (2011) 8-10. Pe r u z z i , L., Pe t e r s o n , A., Ti s o n , J.-M. & D. Ha r p k e : New light on phylogeny and taxonomy of the Eurasian Gagea villosa- G. fragifera complex (Liliaceae). Nord. J. Bot. 29 (2011) PhD and Diploma Theses 722-733. 2011 Pe t e r s o n , A., I.G. Le v i c h e v , J. Pe t e r s o n , D. Ha r p k e & M. Sc h n i t t l e r : New Al-Ho d a l i , K.Y.: Systematic revision of the genus Tulipa (Liliaceae) insights into phylogeny and taxonomy of Chinese species in Jordan. (Master Thesis) University of Jordan, Amman of Gagea (Liliaceae) ‑ Speciation through hybridization. (2011) 139 pp. Organisms Divers. Evol. 11 (2011) 387-407. Ba i e r , C.: Comparative phylogeographic and population genetic analyses of three tropical pioneer trees, Macaranga winkleri, M. winkleriella and M. tanarius (Euphorbiaceae). (PhD Thesis) Martin-Luther-Universität Halle-Wittenberg, Halle/S. (2011) 156 pp. Be n o r , S.: Phylogeny of the genus Corchorus (Malvaceae s.l.) and diversity analyses in selected species: evidence from morphology, flow cytometry, and molecular data. (PhD Thesis) Universität Kassel, Kassel (2011) 125 pp. Nü r k , N.: Phylogenetic analyses in St. John’s wort (Hypericum) - inferring character evolution and historical biogeography. (PhD Thesis) Freie Universität Berlin, Berlin (2011) 129 pp.

51 Abteilung Genbank/Department of Genebank

Research Group: Taxonomy of Plant Genetic Resources Head: Dr. Frank Blattner (provisional)

Scientists were added to the collection. Apart of employees of the IPK the collections were used by many visitors of the IPK facilities, IPK financed and vouchers were sent abroad in the frame of national and Pistrick, Klaus, Dr. international herbarium exchange. This part of the work involves also taxonomical determination of genebank materials Visiting Scientists/Scholars from a wide variety of plant families (K. Pistrick). Filatenko, Anna, Dr. (self-financed, 15.02.-10.03.2010) Fritsch, Reinhard, Dr. (DFG) A general review of the nomenclatural relationships between diverging recent taxonomic treatments of Triticum- and × Triticosecale variability, following broad versus narrow Goals species concepts, has been performed. In Triticum a detailed infraspecific system has been supported for stable classification, Curatorial management of living and archive taxonomic better estimation of genetic erosion and suitable management collections, investigation of morphological, karyological and of wild and cultivated Triticum accessions in genebanks. It is anatomical characters resulting in phylogenetic and taxonomic proposed to maintain × Triticosecale Wittm. as a nothogenus, conclusions, and nomenclaturally suitable treatments of with × T. rimpaui Wittm. for octoploid races, × T. neoblaringhemii taxa. The studies target general questions of the taxonomy of A. Camus for hexaploid races and × T. semisecale (Mac Key) crop plants and are performed in close cooperation with the K. Hammer et A. Filat. for tetraploid races (K. Hammer, A.A. research group Experimental Taxonomy. Filatenko, K. Pistrick).

The actual situation of plant genetic resources in southeastern Research Report Georgia has been studied together with staff members of the Botanical Garden and Institute of Botany, Tbilisi in July 2010. The custodial management of the taxonomic reference 294 accessions of local cereals, pulses, spices and medicinal collections is a continuous activity of the research group. plants could be collected, despite of increased genetic erosion, During 2010 and 2011 7,693 herbarium sheets, 1,955 fruit especially in field crops. Variable landraces of Triticum aestivum and seed samples, and more than 1,056 cereal spike samples L. are still cultivated in several villages of Mescheti. Nine

Fig. 20 Spikes of different Triticum and ×Triticosecale accessions collected in Mescheti (southeastern Georgia) 2010: a – Triticum carthlicum Nevski var. stramineum Zhuk.; b – T. carthlicum Nevski var. carthlicum; c – T. aestivum L. var. pseudoerythrospermum (Kudr.) A. Filat.; d, e – different types of T. aestivum L. var. aestivum; f, g – different types of T. aestivum L. var. variabile (Kudr.) A. Filat.; h – T. aestivum L. var. ferrugineum (Alef.) Mansf.; i – T. aestivum L. var. ferrugineumcompactoides (Kob.) Mansf.; j – T. aestivum L. var. pulchrum (Kudr.) A. Filat.; k – T. aestivum L. var. lutescens (Alef.) Mansf.; l – T. aestivum L. var. uzbekistanicum (Kudr.) A. Filat.; m – T. aestivum L. var. milturum (Alef.) Mansf.; n, o – different types of ×Triticosecale neoblaringhemii A. Camus (M. Akhalkatsi, J. Ekhvaia, K. Pistrick).

52 different varieties of subspecies aestivum, as well as of subsp. 2011 hadropyrum (Flaksb.) Tzvel. have been recorded in the fields Gl a d i s , T. & K. Pistrick: Chaerophyllum byzantinum Boiss. and (see Fig. 20, p. 52). Relic crops, such as Pisum sativum L. (pod Trachystemon orientalis (L.) G. Don – recently introduced vegetable), Glycine max (L.) Me r r . (coffee substitute and cattle- from Turkish wild flora as new crop species among other food) or Brassica oleracea L. var. viridis (leaf vegetable) could be interesting findings from immigrant gardens in western collected in Atčara (M. Akhalkatsi, J. Ekhvaia, K. Pistrick). Germany. Genet. Resour. Crop Evol. 58 (2011) 165-174. Ha m m e r , K., A.A. Fi l a t e n k o & K. Pistrick: Remarks on Triticum L. and During several DFG-funded research missions in Iran new × Triticosecale Wittm. – Genet. Resour. Crop Evol. 58 (2011) Allium materials were collected and incorporated in the living 3-10. Allium reference collection of the IPK. Analyses of these Ku s t e r e r , J., R.M. Fr i t s c h & M. Ke u s g e n : Allium species from Central plants resulted in the detection of several new species. The and Southwest Asia are rich sources of marasmin. J. Agr. phylogenetic positions of these newly discovered taxa could Food Chem. 59 (2011) 8289-8297. all be determined by molecular markers. For a part of these Pistrick, K. & K. Ha m m e r : Peter Hanelt 80. Genet. Resour. Crop Evol. species taxonomic descriptions and determination keys were 58 (2011) 1-2. already published (R. Fritsch). Ra z y f a r d , H., S. Za r r e , R.M. Fr i t s c h & H. Ma r o o f i : New species of Allium L. sect. Acanthoprason Wendelbo (Alliaceae) from Formerly published molecular phylogenies of the members of Iran. Ann. Bot. Fenn. 48 (2011) 352-360. Allium subgenus Melanocrommyum based on nuclear rDNA internal transcribed spacer sequences were supplemented by additional materials from Central Asia, West Asia, and Near Books and Book Chapters East. This work resulted in further clarification of relationships in subgenus Melanocrommyum. Several taxonomic groups 2011 were already newly described, and a modern conspectus of Pistrick, K.: Kulturpflanzen. In: Jä g e r , E. (Ed.): Rothmaler Exkur- subg. Melanocrommyum was published. An illustrated key for sionsflora von Deutschland. Gefäßpflanzen: Grundband. 20., determination of all sections and subsections of this subgenus neu bearbeitete und erweiterte Auflage. Spektrum Akad. is ready for publication, and description of several new species Verlag, Heidelberg (2011) 930 pp. from Near East is in preparation (R. Fritsch, M. Gurushidze).

Other Papers 2010 Publications Pistrick, K., M. Ak h a l k a t s i , M. Girgvliani & T. Shanshiashvili : Sammlung von Saat- und Pflanzgut pflanzengenetischer Ressourcen Peer Reviewed Papers in Svanetien und Lečchumi (Georgien, Kaukasus) 2009. Berichte der Gesellschaft für Pflanzenbauwissenschaften, 2010 Bd. 5 (2010) 123-127. Fr i t s c h , R.M., F.R. Bl a t t n e r & M. Gu r u s h i d z e : New classification of Allium L. subg. Melanocrommyum (Webb & Berthel.) Rouy (Alliaceae) based on molecular and morphological Additional Publications 2009 characters. Phyton 49 (2010) 145-220. Fr i t s c h , R.M. & M. Gu r u s h i d z e : Phylogenetic relationships of Fr i t s c h , R.M. & H. Ma r o o f i : New species and new records of Allium ornamental species in Allium L. subg. Melanocrommyum L. (Alliaceae) from Iran. Phyton 50 (2010) 1-26. (Webb et Berthel.) Rouy (Alliaceae). Isr. J. Plant Sci. 57 (2009) Gu r u s h i d z e , M., R.M. Fr i t s c h & F.R. Bl a t t n e r : Species-level phylogeny 287-295. of Allium subgenus Melanocrommyum: Incomplete lineage sorting, hybridization and trnF gene duplication. Taxon 59 (2010) 829-840. Ho f f m a n n , M.H., H. Sc h m u t h s , C. Ko c h , A. Me i s t e r & R.M. Fr i t s c h : Comparative analysis of growth, genome size, chromosome numbers and phylogeny of Arabidopsis thaliana and three cooccurring species of the Brassicaceae from Uzbekistan. J. Bot. 2010 (2010) Article ID 504613, 8 pages, doi:10.1155/2010/504613. Ko t s e r u b a , V., K. Pistrick, F.R. Bl a t t n e r , K. Ku m k e , O. We i s s , T. Ru t t e n , J. Fu c h s , T. En d o , S. Na s u d a , A. Gh u k a s y a n & A. Ho u b e n : The evolution of the hexaploid grass Zingeria kochii (Mez) Tzvel. (2n=12) was accompanied by complex hybridization and uniparental loss of ribosomal DNA. Mol. Phylogenet. Evol. 56 (2010) 146-155.

53 Wahlweise: Kapitel, Thema, Unterthema, Autor, Zusatzinformationen Abteilung Cytogenetik und Genomanalyse/ Department of Cytogenetics and Genome Analysis

Abb. 21 Fig. 21 Anaphase-Chromosomen eines stabilen (links) und eines instabilen (rechts) Anaphase chromosomes of a stable (left) and an unstable (right) Hordeum Hordeum vulgare × H. bulbosum Hybridembryos nach Immunmarkierung mit vulgare × H. bulbosum hybrid embryo after immunostaining with anti-CENH3 anti-CENH3- und anti-Tubulin-Antikörpern. In instabilen Hybriden sind die and anti-tubulin. In the unstable hybrid, the centromeres of lagging chromo- Zentromeren der nicht verteilten Chromosomen (Pfeilspitze) frei von CENH3 somes (arrowed) are CENH3-negative which indicates centromere inactivity. und somit inaktiv. Diese Chromosomen werden nicht in die Tochterkerne The lagging chromosomes are not integrated into daughter nuclei, form mi- integriert, bilden stattdessen Kleinkerne und werden abgebaut. Im Ergebnis cronuclei and undergo degradation. As a result, a haploid plant is formed (M. dessen entstehen haploide Pflanzen (M. Sanei, R. Pickering, A. Houben). Sanei, R. Pickering, A. Houben).

54 Abteilung Cytogenetik Department of Cytogenetics und Genomanalyse and Genome Analysis Leiter: Prof. Dr. Ingo Schubert Head: Prof. Ingo Schubert

Allgemeine Forschungsziele Research Goals Die Forschungsschwerpunkte der Abteilung sind die Ge- The research topics of the Department are focused on genome nomdynamik auf molekularer und mikroskopischer Ebene dynamics at the molecular and the microscopic level under unter evolutionären, ontogenetischen und experimentellen evolutionary, developmental and experimental aspects Gesichtspunkten (vor allem Bereich Cytogenetik) sowie die Ge- (in particular Programme Cytogenetics) as well as on the netik pflanzlicher Leistungen unter Einbeziehung genomwei- genetic dissection of crop plant’s performances using forward ter, vorwärts- und revers-genetischer sowie bioinformatischer and reverse genetic as well as bioinformatic approaches Ansätze (vor allem Bereich Genomanalyse). (predominantly Programme Genome Analysis).

Folgende Themenbereiche werden gruppen-/abteilungsüber- The Department’s research groups are working on the follow- greifend bearbeitet: ing topics:

Bereich Cytogenetik (Leiter: Prof. Dr. Ingo Schubert) Programme Cytogenetics (Head: Prof. Ingo Schubert) − Evolution von Gen- und Chromosomenbeständen im Zusam- − Evolution of genes and chromosome complements in the menhang mit Polyploidisierung, Artbildungsprozessen und context of polyploidisation, speciation and sexual versus sexuellen versus asexuellen Reproduktionswegen (Arbeits- asexual propagation (research groups Karyotype Evolution, gruppen Karyotypevolution, Chromosomenstruktur und Chromosome Structure and Function, Genome Plasticity, -funktion, Genomplastizität, Epigenetik und Apomixis sowie Epigenetics, Apomixis, and at the Dept. Physiology and Cell Pflanzliche Reproduktionsbiologie ‑ Abt. Physiologie und Biology the group Plant Reproductive Biology). Zellbiologie); − Generation of minichromosomes as gene transfer vehicles − Erzeugung von Minichromosomen und von künstlichen and of artificial centromeres, as well as approaches for Zentromeren sowie Ansätze zum gezielten Gentransfer in gene targeting in barley (research groups Chromosome Gerste (Arbeitsgruppen Chromosomenstruktur und -funk- Structure and Function, Karyotype Evolution, Epigenetics, tion, Karyotypevolution und Epigenetik sowie Pflanzliche and the Dept. Physiology and Cell Biology the group Plant Reproduktionsbiologie ‑ Abt. Physiologie und Zellbiologie); Reproductive Biology). − Mechanismen und funktionelle Bedeutung epigenetischer − Mechanisms and functional importance of epigenetic Prozesse (v. a. DNA-Methylierung und Histon-Modifikatio- processes (e.g. DNA methylation and histone modifications) nen) für die Assemblierung kompetenter versus inkompe- for the assembly of competent versus incompetent chromatin tenter Chromatinzustände, z. B. Heterochromatin, Zentromer states (heterochromatin, centromere etc.) (research groups (Arbeitsgruppen Epigenetik, Karyotypevolution, Chromoso- Epigenetics, Karyotype Evolution, Chromosome Structure menstruktur und -funktion, Genomplastizität und Apomixis, and Function, Genome Plasticity, Apomixis and of the Dept. sowie Dateninspektion - Abteilung Molekulare Genetik); Molecular Genetics the group Data Inspection). − Entwicklungsabhängige und experimentell (Mutanten, − Developmental and experimentally induced (mutants; DNA DNA-Schäden) erzeugte Genomdynamik (z. B. hinsichtlich damage) genome dynamics and its biological consequences der Interphasechromatinanordnung oder chromosomaler (altered interphase chromatin arrangement; chromosome Strukturumbauten) und deren biologische Konsequenzen rearrangements) (research group Karyotype Evolution). (Arbeitsgruppe Karyotypevolution). Programme Genome Analysis (Head: Dr. Patrick Schweizer) Bereich Genomanalyse (Leiter: Dr. habil. Patrick Schweizer) − Identification and exploitation of the natural genetic diversity − Molekulare Identifikation von Genen bzw. Allelen für agro- for improvement of agriculturally important traits in cereals nomisch bedeutsame Eigenschaften in Getreide (Arbeits- (research groups Transcriptome Analysis, Gene and Genome gruppen Transkriptomanalyse, Gen- und Genomkartierung, Mapping, Bioinformatics and Information Technology). Bioinformatik und Informationstechnologie); − Analysis of gene- and allele-phenotype relationships and − Gen- und Allel-Phänotypbeziehungen sowie Genexpres- of gene expression patterns in cereals for (a)biotic stress sionsmuster in Getreidepflanzen, die unter (a)biotischem resistance and other yield-related traits (research groups Stress leiden, Resistenzen aufweisen oder sich in anderen Transcriptome Analysis, Gene and Genome Mapping, ertragsrelevanten Merkmalen unterscheiden (Arbeitsgrup- Bioinformatics and Information Technology). pen Transkriptomanalyse, Gen- und Genomkartierung, Bio- informatik und Informationstechnologie);

55 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

− Etablierung von Ontologien bzw. kontrollierten Vokabulari- − Establishing of ontologies and controlled vocabularies for en zur Strukturierung, Integration und Vernetzung diverser structuring, integration and linking of diverse databases; Datenbanken, Assemblierung und Annotation genomischer assembly and annotation of genomic sequences (research Sequenzen (Arbeitsgruppe Bioinformatik und Informations- group Bioinformatics and Information Technology). technologie). In addition to obtaining basic knowledge, it is intended to Im Mittelpunkt der Arbeiten stehen neben Erkenntnisgewinn establish the prerequisites for directed modification of plant die Schaffung von Voraussetzungen für eine gezielte Modifika- genomes and to provide technological platforms and resources tion pflanzlicher Genome sowie die Etablierung und Verbreite- for biotechnology and breeding purposes. These efforts are rung biotechnologisch und züchterisch nutzbarer Techniken largely integrated within the frame of the Plant Genome und Ressourcen. Diese Arbeiten finden zu einem wesentlichen Resources Centre (PGRC) involving all departments of the Teil im Rahmen des Pflanzengenom-Ressourcen-Centrums IPK. PGRC services such as DNA sequencing and bioinformatics (PGRC) statt, einer abteilungsübergreifenden Forschungs- und services are provided by the research groups Transcriptome Dienstleistungsplattform. Im PGRC-Dienstleistungsbereich, Analysis and Bioinformatics and Information Technology. der in den Arbeitsgruppen Transkriptomanalyse, Bioinformatik und Informationstechnologie verankert ist, werden u. a. DNA- Special germplasm collections (barley, field bean, and other Sequenzierung und bioinformatischer Service angeboten. crops) with gene and chromosome mutations are developed, characterised and maintained within the framework of the Im Rahmen der gruppenspezifischen Forschungsarbeiten wird research programmes of the research groups Chromosome die Erhaltung und Weiterentwicklung von Spezialsortimenten Structure and Function, Gene and Genome Mapping and v. a. der Ackerbohne, der Gerste u. a. Gramineen mit modifi- Karyotype Evolution. zierten Gen- und Chromosomenbeständen betrieben (Arbeits- gruppen Chromosomenstruktur und -funktion, Gen- und Ge- nomkartierung, Karyotypevolution). Developments in 2010 and 2011 Due to retirement of the group leader, the group ‘In vitro Differentiation’ has been closed on 31.12.2010. Several projects Entwicklung im Berichtszeitraum are continued in the Junior Research Group “Stem Cell Research” Mit Eintritt der Leiterin in den Ruhestand wurde die Arbeits- at the Institute of Anatomy and Cell Biology, University Halle, gruppe ‚In vitro-Differenzierung’ zum 31.12.2010 geschlos- led by the former group member Dr. Insa Schroeder. sen. Mehrere Projekte werden in der Junior-Forschergruppe „Stammzell-Forschung“ am Institut für Anatomie und Zellbio- In 2010 and 2011 one habilitation, eight dissertations (PhD), logie der Martin-Luther-Universität Halle-Wittenberg weiterge- one Diploma thesis and two Bachelor theses have been finished führt, die von der früheren IPK-Mitarbeiterin Dr. Insa Schroeder successfully. geleitet wird. Besides PGRC services, flow-cytometry, fluorescence micros- In den Jahren 2010/2011 wurden eine Habilitation, acht Disser- copy, high-throughput transient RNAi and bioinformatic ap- tationen, eine Diplomarbeit und zwei Bachelorarbeiten erfolg- proaches were important issues for collaboration within the reich abgeschlossen. Department and with other groups at and beyond IPK during the last two years. All groups of the Department collaborated Für die abteilungsinterne, die institutsweite und die instituts- with internal and external partners within the frame of large übergreifende Zusammenarbeit spielten auch 2010/2011 laser- national and international research networks such as GABI gestützte Durchflusszytometrie, Fluoreszenzmikroskopie, tran- (BMBF), “Pakt für Forschung und Innovation” (WGL), Excellence siente Hochdurchsatz-RNAi und Bioinformatik eine wesentliche network (Saxony-Anhalt), ERA-NET Plant Genomics and KBBE Rolle. Alle Gruppen der Abteilung kooperierten innerhalb und projects. For the multiple cooperative links of the individual außerhalb des IPK, z. B. im Rahmen von GABI, des „Paktes für groups see their detailed reports and publication records. Forschung und Innovation“ der WGL, des Exzellenz-Netzwerks des Landes Sachsen-Anhalt, des ERA-NET Plant Genomics und The following scientific achievements are considered as high- von KBBE-Projekten. Für die vielfältig verflochtene Zusammen- lights of the Department in 2010/2011: arbeit zwischen den Gruppen der Abteilung, innerhalb des IPK und darüber hinaus siehe die Berichte der jeweiligen Arbeits- gruppen und deren Publikationsverzeichnisse.

Unter den in 2010/2011 erbrachten Forschungsleistungen seien die folgenden besonders hervorgehoben:

56 Abb. 22 Chromosomenverkürzung durch Telomerentnahme in tetraploider Gerste (2n=28). A) T-DNA Konstrukt mit Selektionsmarker (hpt), Rekombinationsbox (Cre/lox) und Telomerwiederholung (TEL). B) Idiogramm für Gerste mit Position der Selektionsmarker und Bruchpunkten für die Verkürzung und Telomerentnahme. C) Metaphasenkern bei tetraploider Gerste nach Fluoreszenz in situ-Hybridisierung (FISH) mit 5S rDNA und subtelomeren Proben, die einen der vier in der Nähe des Zentromers verkürzten 2H-Chromosomenarme zeigen. D) Wildtyp und verkürzte 2H-Chromosomen nach FISH mit sechs speziellen Proben (L. Ma). Fig. 22 Chromosome truncation by telomere seeding in tetraploid barley (2n=28). A) T-DNA construct with selection marker (hpt), recombination box (Cre/lox) and telomere repeat block (TEL). B) Idiogramme of barley indicating the position of marker sequences and the breakpoints for truncation and telomere seeding. C)Tetraploid barley metaphase nucleus after fluorescence in situ hybridisation (FISH) with 5S rDNA and subtelomeric probes showing one out of four chromosomes 2H truncated in its long arm close to the centromere. D) Wild-type and truncated 2H chromosomes after FISH with six specific probes (L. Ma).

Erfolgreiche Manipulation von Pflanzenchromosomen Successful generation of engineered plant chromosomes Im Rahmen eines gemeinsamen „Pakt für Forschung und In frame of a joined “Pakt für Forschung und Innovation” Innovation“-Projektes der Leibniz-Gemeinschaft generierten project, the research groups Epigenetics, Karyotype Evolution, die Gruppen Epigenetik, Karyotypevolution, Chromosomen- Chromosome Structure and Function, and Plant Reproductive struktur und -funktion und Pflanzliche Reproduktionsbiologie Biology (Dept. of Physiology and Cell Biology) generated engi- (Abt. Physiologie und Zellbiologie) verkürzte Chromosomen neered minichromosomes of Arabidopsis thaliana and barley. in Arabidopsis thaliana und in Gerste über Agrobakterium-ver- Following a top-down approach, truncated endogenous chro- mittelten Transfer von Telomersequenz-haltigen Konstrukten. mosomes were obtained by Agrobacterium-mediated transfer Telomersequenz-bedingte Chromosomenverkürzung wurde of T-DNA constructs containing telomere sequences. Telomere nur in tetraploiden Pflanzen gefunden.O ffenbar ist der Verlust seeding connected to chromosome truncation was found in chromosomaler Segmente nur in polyploidem Hintergrund tetraploid plants only, indicating that genetic redundancy fa- tolerierbar. Die verkürzten Chromosomen sind mitotisch stabil cilitates recovery of shortened chromosomes. Truncated chro- und sexuell übertragbar, obwohl die Übertragungsrate durch mosomes were transmissible in sexual reproduction, but were männliche wie weibliche Gameten geringer ist als nach den via male and female gametes inherited at rates lower than Mendelschen Regeln zu erwarten (C.H. Teo, L. Ma, E. Kapusi, G. expected according to Mendelian rules. However, more stud- Hensel, I. Schubert, J. Kumlehn, A. Houben, M.F. Mette; Plant ies are required to develop tools for a targeted shortening of Journal 2011; E. Kapusi, L. Ma, C.H. Teo, G. Hensel, A. Himmel- chromosome arms (C.H. Teo, L. Ma, E. Kapusi, G. Hensel, I. Schu- bach, I. Schubert, M.F. Mette, J. Kumlehn, A. Houben; Chromo- bert, J. Kumlehn, A. Houben, M.F. Mette; Plant Journal 2011; E. soma 2011; Abb. 22). Kapusi, L. Ma, C.H. Teo, G. Hensel, A. Himmelbach, I. Schubert, M.F. Mette, J. Kumlehn, A. Houben; Chromosoma 2011; Fig. 22).

57 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Abb. 23 Der C-terminale Teil der zentromerischen Histon- Variante CENH3 ist notwendig und hinreichend für die Aufnahme zentromerischer Nukleosomen während der G2-Phase des mitotischen Zyklus: der N-terminale Teil ist zusätzlich notwendig zur Ablagerung der meiotischen Nuclei an die Zentromere. A) Schema der Zusammensetzung der C- und N-terminalen Teile von CENH3 mithilfe von EYFP-Kennzeichnung. Der N-terminale Teil führt zu diffusen Signalen wohingegen der C-terminale Teil sich um Zentromere in somatischen transgenen Arabidopsis thaliana-Nuclei nachweisen lässt. B) Mitotischer Vorphasennucleus mit doppelten Signalen aufgrund von Schwesterkinetochoren. C) Meiotische Vorphasenkern mit Immunosignalen für CENH3 nur im Bereich der geclusterten Zentromere aber ohne EYFP-Signale für den C-terminalen Teil (I. Lermontova). Fig. 23 The C-terminal part of the centromeric histone H3 variant CENH3 is necessary and sufficient for loading of centromeric nucleosomes during G2 of the mitotic cycle; the N-terminal part is required for deposition at centromeres of meiotic nuclei. A) Scheme of constructs of N- and C-terminal parts of CENH3 each labelled by EYFP. The N-terminal part results in diffuse signals whereas the C-terminal part targets centromeres in somatic transgenic A. thaliana nuclei. B) Mitotic prophase nucleus with double signals representing sister kinetochores. C) Meiotic prophase nucleus with immunosignals only for CENH3 at clustered centromeres, but without EYFP signals for the C-terminal part (I. Lermontova).

Erzeugung haploider Pflanzen durch Instant generation of haploid plants uniparentale Chromosomeneliminierung via chromosome elimination Der Verlust eines elterlichen Genoms nach interspezifischer Chromosome elimination - the removal of one parental genome Befruchtung ist oft eine Folge zwischenartlicher Kreuzungen. after an egg is fertilised by sperm from another species - is Im Ergebnis entstehen haploide Embryonen, die nur die Hälfte one consequence of an interspecific hybridisation event. This des normalen Chromosomenbestandes aufweisen. Obwohl die process results in the formation of haploid embryos, which Eliminierung der Chromosomen eines Elters nach interspezifi- have half the normal number of chromosomes. Chromosome scher Kreuzung seit ca. 40 Jahren zur Erzeugung von Haploiden elimination has been exploited in barley and other species für Kartierungs- und Züchtungszwecke genutzt wird, sind die to produce doubled haploids for breeding and mapping dafür verantwortlichen zellulären Mechanismen noch wenig purposes. Although this process was first described already verstanden. Die Gruppe Chromosomenstruktur und -funktion 40 years ago, the actual cellular mechanism involved in the entdeckte, dass der uniparentalen Chromosomeneliminierung process of uniparental chromosome elimination remains poorly in Gerste-Hybriden ein Verlust der zentromerischen Histonvari- understood. The research group Chromosome Structure and ante CENH3 in den betroffenen Chromosomen vorausgeht (s. Function revealed that uniparental chromosome elimination in Abb. 21, S. 54). Auch in chromosomal stabilen Artkombinatio- interspecific barley hybrids is preceded by the loss of CENH3 nen werden nicht alle elterlichen CENH3-Varianten in die Zen- from centromeres. In stable species combinations not all tromere eingebaut, obwohl alle entsprechenden Transkripte parental CENH3 variants are incorporated into centromeres nachweisbar sind. Diese Befunde werden zur Etablierung effizi- despite the presence of CENH3 transcripts from both parents enter Methoden der Haploidenerzeugung beitragen. (M. Sanei, (see Fig. 21, p. 54). These findings will help to establish more R. Pickering, K. Kumke, S. Nasuda, A. Houben; Proc. Natl. Acad. efficient methods for generating haploids (M. Sanei, R. Sci. U.S.A., highlighted in Faculty of 1000, Biology 2011). Pickering, K. Kumke, S. Nasuda, A. Houben; Proc. Natl. Acad. Sci. U.S.A., highlighted in Faculty of 1000, Biology 2011).

58 Neue Funktionen der Histonvariante CENH3 bei der New roles of the histone variant CENH3 Aufrechterhaltung von Zentromeren for centromere maintenance Das Zentromer ist die Chromosomenposition, an der bei allen The centromere is the chromosomal position where the kineto- Eukaryoten das Kinetochore, ein Multiproteinkomplex für die chore, a complex multiprotein structure, required for the proper korrekte Segregation von Chromosomen während der Mito- segregation of chromosomes during mitosis and meiosis in all se und der Meiose, ausgebildet wird. Die Mechanismen für eukaryotes, is located. The mechanism(s) that determine cen- die Bestimmung der Zentromeridentität, den Aufbau und die tromere identity, assembly and maintenance of kinetochores Aufrechterhaltung der Kinetochore sind noch weitgehend un- are poorly understood. An important role in kinetochore as- verstanden. Der zentromerischen Histon H3-Variante, CENH3, sembly and function plays the centromeric histone H3 variant kommt eine wichtige Rolle bei der Assemblierung und Auf- CENH3. rechterhaltung von Kinetochoren zu. Nach dem Beweis, dass After proving that CENH3 loading onto centromeric nucleo- die Beladung zentromerischer Nukleosomen bei Pflanzen wäh- somes occurs during late G2 in plants (Lermontova et al. 2006, rend der späten G2-Phase des Zellzyklus erfolgt (Lermontova 2007, 2011a), we showed that RNAi-mediated CENH3 knock- et al. 2006, 2007, 2011a), konnte in Zusammenarbeit mehrerer down in Arabidopsis thaliana caused dwarfism by a reduced Gruppen gezeigt werden, dass RNAi-bedingte CENH3-Vermin- number of mitoses (the remaining mitoses seemed to be error- derung in Arabidopsis thaliana zu Zwergwuchs durch reduzierte free) as well as reduced fertility. Mitosezahlen (die verbleibenden Mitosen verlaufen fehlerfrei) Whereas the C-terminal part of CENH3 is essential and sufficient sowie zu verminderter Fertilität führt. Während der C-terminale for correct mitotic divisions, the N-terminal part is required for Teil von CENH3 notwendig und ausreichend für die mitotische CENH3 deposition at centromeres of meiotic nuclei (see Fig. 23, Teilung ist, ist der N-terminale Teil für den Einbau von CENH3 in p. 58). Expression of N-terminally truncated CENH3 can reduce die Zentromere meiotischer Kerne unverzichtbar. Die Expressi- the amount of endogenous CENH3, mimicking the effect of on von N-terminal verkürztem CENH3 kann die Menge von en- RNAi. The consequences of reduced endogenous CENH3 and of dogenem CENH3 verringern und so einen RNAi-Effekt simulie- lack of meiotic incorporation of N-terminally truncated CENH3 ren. Die Folge von vermindertem endogenem CENH3 ist verrin- are reduced fertility due to insufficient CENH3 loading to the gerte Fertilität aufgrund unzureichender Beladung der Zentro- centromeres of meiotic chromosomes, subsequent lagging of mere mit CENH3 und nachfolgendem ‚Chromosomen-lagging’ chromosomes and formation of micronuclei (I. Lermontova, und Kleinkernbildung (I. Lermontova; O. Koroleva, John Innes O. Koroleva, John Innes Centre, Norwich, UK; T. Rutten, Dept. Centre, Norwich, UK; T. Rutten, Abt. Physiologie und Zellbiolo- of Physiology and Cell Biology; J. Fuchs, V. Schubert, I. Moraes, gie; J. Fuchs; V. Schubert; I. Moraes; D. Koszegi, Abt. Molekulare D. Koszegi, Dept. of Molecular Genetics; I. Schubert; Plant J. Genetik; I. Schubert; Plant J. 2011) (s. Abb. 23, S. 58). 2011).

Evolution eines parasitären überzähligen B-Chromosoms Evolution of a selfish supernumerary chromosome revealed B-Chromosomen sind für Wachstum und Entwicklung eines B chromosomes are not required for the normal growth and Organismus nicht erforderlich. Dennoch werden sie in allen development of organisms, yet they are found in all eukaryotic eukaryotischen Stämmen gefunden. Sie repräsentieren eine phyla and are assumed to represent a specific type of selfish besondere Form des Vorkommens parasitärer DNA. In Zusam- DNA. In close collaboration between the groups Chromosome menarbeit zwischen den Gruppen Chromosomenstruktur und Structure and Function, Bioinformatics and Information -funktion, Bioinformatik und Informationstechnologie, Karyo- Technology, Karyotype Evolution, Experimental Taxonomy and typevolution, Experimentelle Taxonomie und Genomdiversität Genome Diversity (the two latter Department of Genebank) (die letzten beiden Abteilung Genbank) wurden unerwartet we found that the B chromosomes of rye are unexpectedly viele von Genen abgeleitete Sequenzen auf dem B-Chromo- rich in gene-derived sequences, which allowed to trace their som des Roggens gefunden, die es erlaubten, ihre ursprüng- origin to fragments of several A chromosomes. Compared to liche Position auf den A-Chromosomen zurückzuverfolgen. A chromosomes (As), the B chromosomes (Bs) were found to Verglichen mit den A-Chromosomen akkumuliert das B-Chro- accumulate large numbers of specific repeats and insertions mosom hohe Zahlen spezifischer Repeats undI nsertionen von of organellar DNA. The origin of rye Bs was estimated at Organellen-DNA. Die Entstehung des Roggen-B-Chromosoms approximately 1.1 to 1.3 million years ago, during radiation of konnte auf 1,1 - 1,3 Mio. Jahre zurück datiert werden, d. h., the genus Secale (1.7 million years ago) (see Fig. 27, p. 67). etwa in die Zeit der Radiation der Gattung Secale vor 1,7 Mio. Jahren (s. Abb. 27, S. 67).

59 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Abb. 24 Das Expressionsniveau der für Apomixis verantwortlichen Kandidatenallele in mikrodis- sektierten lebenden Eizellen (Pfeile deuten auf Megaspo- ren-Mutterzellen) aus 5 diploiden sich geschlechtlich fortpflanzenden (S) und 8 diploiden apomiktischen (A) Boechera-Genotypen. Das Niveau wurde mithilfe von qRT-PCR im Vergleich zum Ubiquitin-Gen gemessen (J. Corral). Fig. 24 Expression level of apomixis candidate allele, using qRT- PCR relative to the UBQ housekeeping gene, in microdissected live ovules (arrows show megaspore mother cells) from 5 diploid sexual (S) and 8 diploid apomictic (A) Boechera genotypes (J. Corral).

Ein Apomixis-spezifisches Allel wird ausschließlich in Ovulen An apomeiosis-specific allele is exclusively apomiktischer Boechera-Pflanzen exprimiert expressed in apomictic Boechera ovules Ein durchflusszytometrischer Hochdurchsatz-Samen-Screen Using high-throughput seed screening, we analysed >24,000 für >24.000 Einzelsamen von 110 Boechera-Akzessionen er- single seeds in 110 Boechera accessions, and have shown gab genotypspezifische quantitative Unterschiede für die drei genotype-specific quantitative variation for the three apomixis Apomixis-Komponenten Apomeiose, Parthenogenese und components apomeiosis, parthenogenesis and pseudogamy. Pseudogamie. Außer unreduzierten Eizellen (Apomeiose) pro- Besides unreduced egg cells (apomeiosis), Boechera also duziert Boechera häufig auch unreduzierte Pollen. Möglicher- produces frequently unreduced pollen. Possibly, similar weise werden beide Phänomene durch ähnliche Mechanismen mechanisms control both processes. A pipeline has been kontrolliert. Aus Sequenzen normalisierter blütenspezifischer established to compare expression profiles of microdissected cDNA-Bibliotheken wurden ‚high-density microarrays’ erstellt live gametophytic tissue between sexual and apomictic und zum Vergleich der Expressionsprofile aus lebendem, mi- Boechera. High-density microarrays were built using sequences krodissektiertem gametophytischem Gewebe sexueller und generated from flower-specific normalised cDNA libraries. apomiktischer Akzessionen eingesetzt. Diese Analysen führten These analyses eventually led to the identification of a single zur Identifizierung je eines Gens für die Bildung von unredu- gene each underlying unreduced egg (J. Corral) and pollen zierten Eizellen bzw. Pollen. Das Kandidatengen für Apomeiose (M. Mau) formation. The candidate apomeiosis gene has led zeigte einen spezifischen Polymorphismus in 12 apomiktischen to the identification of a single “apomixis polymorphism” in Akzessionen unterschiedlicher geografischer Herkunft, der in 12 geographically distant apomictic accessions, but not in any keiner der getesteten sexuellen Akzessionen auftrat. Dieser tested sexual accession. This polymorphism appears to have Polymorphismus scheint im Laufe der Evolution der Gattung undergone recombination during the evolution of Boechera, Boechera Rekombination erfahren zu haben, kommt jedoch in but is shared by all apomicts, regardless of genetic, ploidy or allen Apomikten, unabhängig vom genetischen, geografischen geographic backgrounds. The female “apomixis allele” has been oder Ploidie-Hintergrund, vor. Die ausschließliche Expression verified by qRT-PCR showing exclusive expression in apomictic des weiblichen ‚Apomixis-Allels’ in apomiktischen Ovulen wur- ovules (Fig. 24). de durch qRT-PCR bestätigt (Abb. 24).

60 Abb. 25 Die reduzierte pilzliche Aus- breitung und Hyphenwachs- tum von Blumeria graminis f.sp. tritici auf Weizenpflanzen, die das Virus-induzierte Gen Silencing von BgGTF1 und BgGTFII zeigen. ***, Signifikan- te Unterschiede im Vergleich zur leeren Vektorkontolle (BSMV:00) mit p < 0,0001. Die Durchschnitts- und Median- werte aus 2-3 unabhängigen Experimenten mit insgesamt 77-102 Pflanzen (D. Nowara). Fig. 25 Reduced fungal penetration and hyphal growth of Blumeria graminis f.sp. tritici on wheat plants exhibiting virus-induced gene silencing of BgGTF1 and BgGTFII, respectively. ***, significantly different from empty-vector control (BSMV:00) with p < 0.0001. Mean and median values from 2-3 independent experiments comprising in total 77-102 plants are shown (D. Nowara).

Wirts-induziertes ‚Gen-Silencing’ in phytopathogenen Pilzen Host-induced gene silencing in phytopathogenic fungi Phytopathogene Pilze sind die wichtigste Gruppe von Pflanzen- Phytopathogenic fungi represent the most important group of parasiten und verursachen weltweit ernorme Schäden an Kul- plant parasitic organisms and cause enormous damage to crop turpflanzen hinsichtlich Ertrag und Qualität. Pflanzen wehren yield and quality worldwide. Plants defend themselves against sich gegen phytopathogene Pilze durch Zellwandverstärkung, invading phytopathogenic fungi by cell-wall fortification, the antimikrobielle Verbindungen und programmierten Zelltod. accumulation of antimicrobial low-molecular-weight com- Wir entdeckten, dass Akkumulation transgener doppelsträn- pounds, antimicrobial proteins and programmed cell death. giger RNA mit Homologie zu pilzlicher Boten-RNA Gerste und We discovered that barley and wheat can also be protected Weizen gegen Attacken durch den Mehltaupilz Blumeria gra- from attack by the powdery mildew fungus Blumeria graminis minis schützt und nannten dieses Phänomen Wirts-induziertes via the accumulation of transgenic, double-stranded RNA tar- Gen-Silencing (HIGS). Transiente Expression verschiedener geting fungal mRNA sequences and named this phenomenon Haarnadel-RNAi-Konstrukte in Gerste-Epidermis verhinder- “host-induced gene silencing” (HIGS). A number of RNAi hair- te das Eindringen der Pilz-Haustorien in die Pflanze. Die Auf- pin constructs expressed transiently in barley epidermal cells nahme der RNA durch das Pathogen erfolgt offenbar wenige stopped the fungus as early as the formation of the first haus- Stunden nach Inokulation, wenn der Pilz gerade im Begriff ist, torium suggesting that uptake of the silencing RNA molecules die Epidermis zu penetrieren. Ähnlich wie transgene Gerste, takes place within few hours after inoculation, when the fun- die ein RNAi-Konstrukt gegen pilzliche Glucanosyltransferase I gus is just about to penetrate the epidermal cell wall. Similar (BgGTFI) trägt, war mit dem ‚barley stripe mosaic virus’ behan- to transgenic barley carrying an RNAi construct against fungal delter Weizen durch Virus-induziertes ‚Gen-Silencing’ der Enzy- glucanosyl transferase I (BgGTFI), BSMV-treated wheat plants ex- me BgGTFI oder BgGTFII signifikant widerstandsfähiger gegen hibiting virus-induced gene silencing of BgGTFI or BgGTFII were B. graminis als unbehandelte Pflanzen A( bb. 25). GTF-Proteine significantly more resistant to B. graminis (see Fig. 25). GTF pro- sind erforderlich für die pilzliche Zellwandbildung. Laufende teins are required for fungal cell-wall formation and elongation. Arbeiten weisen darauf hin, dass HIGS auch gegen Fusarium- Work in progress indicates that HIGS can also be applied to the Befall von Gerste und Weizen einsetzbar ist. Falls HIGS auch na- Fusarium head blight disease of wheat and barley. It remains türlicherweise vorkommt, wäre das gegenwärtige Paradigma to be examined whether HIGS is also a naturally occurring der Wirts-Pathogen-Wechselbeziehung entsprechend zu mo- phenomenon modifying the current paradigm of plant-fun- difizieren (D.N owara, A. Gay, C. Lacomme, J. Shaw, C. Ridout, D. gus communication (D. Nowara, A. Gay, C. Lacomme, J. Shaw, Douchkov, G. Hensel, J. Kumlehn, P. Schweizer; Plant Cell 2010). C. Ridout, D. Douchkov, G. Hensel, J. Kumlehn, P. Schweizer; Plant Cell 2010).

Ingo Schubert, November 2011 Ingo Schubert, November 2011

61 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Programme: Cytogenetics Research Group: Karyotype Evolution Head: Prof. Ingo Schubert Scientists Research Report

IPK financed Centromere research. RNAi-mediated knock-down of Fuchs, Jörg, Dr. the centromeric histone H3 variant CENH3 of Arabidopsis Jovtchev, Gabriele, Dr. (Pakt für Forschung und Innovation, thaliana caused dwarfism by a reduced number of mitoses. 11.01.-10.04.2011; 01.09.-31.10.2011) The remaining mitoses seemed to be error-free. CENH3 RNAi Ma, Lu, Dr. (0.50 Pakt für Forschung und Innovation, transformants revealed reduced fertility because of frequently till 31.10.2011;0.50, 01.11.-31.12.2011) disturbed meiotic chromosome segregation. N-terminally Moraes, Izabel (0.50 / 0.25, till 18.03.2011) truncated EYFP-CENH3 is deposited to and functional within Schubert, Veit, Dr. centromeres of mitotic A. thaliana chromosomes but cannot be loaded to centromeres of meiotic nuclei. Thus, the Grant Positions N-terminal part is required for CENH3 loading during meiosis. Borisova-Todorova, Branimira, Dr. (0.50 Saxony-Anhalt, N-terminally truncated EYFP-CENH3 expression can reduce the till 31.08.2010) amount of endogenous CENH3, mimicking the effect of RNAi. Jovtchev, Gabriele, Dr. (0.50 Saxony-Anhalt, 04.05.-31.08.2010) The consequences of reduced endogenous CENH3 and of lack Lermontova, Inna, Dr. (DFG, since 17.01.2011) of meiotic incorporation of N-terminally truncated EYFP-CENH3 Sulzbacher, Sabine, Dr. (BMBF, 01.01.-31.07.2011) are reduced fertility caused by insufficient CENH3 loading to Watanabe, Koichi, Dr. (BMBF, till 31.12.2010) the centromeres of meiotic chromosomes, subsequent lagging of chromosomes and formation of micronuclei (I. Lermontova; Visiting Scientists/Scholars O. Koroleva, John Innes Centre, Norwich, UK; T. Rutten, Dept. Cincu, Emilia (self-financed, 31.05.-04.06.2010) Physiology and Cell Biology (PCB); J. Fuchs; V. Schubert; I. Moraes; Duc Trung, Tran (MOET-Vietnam, since 17.09.2011) D. Koszegi, Dept. Molecular Genetics (MOG), I. Schubert; Plant Endo, Takashi R., Prof. (IPK, 20.11.-25.11.2010) J. 2011) (Fig. 23, p. 38). Schroeder, Insa, Dr. (Martin Luther University Halle-Wittenberg, 01.01.-30.06.2011) Studies on the regulation of CENH3 transcription in A. thaliana Seeliger, Katharina (self-financed, 11.04.-17.04.2010) revealed within the CENH3 promoter two binding motifs for E2F Temel, Aslihan (Fellowship University of Istanbul, transcription factors which contribute in a concerted manner 04.01.-30.09.2010) to the cell cycle-specific CENH3 expression (S. Heckmann; Vu, Thi Ha Giang, Dr. (self-financed, 25.05.-03.06.2011; I. Lermontova; B. Bergmans, L. de Veylder, Ghent University, since 30.09.2011) Belgium; H. Bäumlein, MOG; I. Schubert; Plant J. 2011). Wobus, Anna M., Prof. (self-financed, since 01.01.2011) Chromatin modifications. Studies on the nuclear distribution of histone methylation marks in dioecious Rumex species Goals revealed that (i) methylation of H3K4me1,2,3 and H3K9me3 is restricted to euchromatin and excluded from the Y Elucidation of structure, plasticity, evolution and epigenetic chromosome-derived heterochromatin; (ii) H3K9me1,2 and modifications of plant genomes and functional chromosome H3K27me1 label the Y chromatin similarily as the autosomal domains. hetero- and euchromatin and (iii) H3K27me2 and H3K27me3

are enriched in Y chromatin of Rumex species with the XX/XY1Y2 sex determination system (Saxony-Anhalt Excellence Cluster, J. Fuchs, G. Jovtchev, B. Borisova-Todorova).

62 Interphase chromatin organisation. Chromosome territory the occurrence of BIR in a higher plant (Vicia faba). However, (CT) arrangement, chromatin domain positions, and sister interstitial asymmetric labelling of sister chromatids indicated chromatid cohesion were studied in relation to the endopoly- DSB repair by gene conversion spanning hundreds of Kb ploidisation degree in A. thaliana. Whereas distinct CTs are (Fig. 26). The apparent loss of heterozygosity after DSB repair mainly maintained, an increasing dispersion of sister chro- in yeast and mammals can be interpreted by unbalanced matids (starting from mid arm positions toward telomeres and segregation of reciprocally translocated chromosomes rather centromeres) accompanies increasing endopolyploidy levels than by the hypothetical BIR (I. Schubert, V. Schubert, J. Fuchs; (V. Schubert, A. Berr, A. Meister, Plant Physiol. 2012). Further- Frontiers in Plant Sci. 2011). more, a similar chromatin organisation was shown for A and B chromosomes of rye (V. Schubert, A. Meister, H. Tsujimoto, T.R. Chromosome engineering. A joint “Pakt für Forschung und Endo, A. Houben; Chromosome Res. 2011). Innovation” project (A. Houben, M.F. Mette, J. Kumlehn, I. Schubert) is aimed to generate barley minichromosomes as Biological consequences of DNA double-strand breaks potential vehicles for gene transfer. Via transformation with (DSB). ‘Break-induced replication’ (BIR), mimicking non- telomere repeat containing constructs, it was possible to reciprocal recombination of regions distal to a DSB, was – based generate truncated chromosomes, which are transmissible on genetic data – claimed to be a mechanism responsible to the next generation, in tetraploid A. thaliana as well as in for repair of >50 % of DSBs in budding yeast. BIR was also tetraploid barley plants (C.H. Teo, L. Ma, E. Kapusi, G. Hensel, J. postulated for mammals without experimental evidence. Kumlehn (PCB); I. Schubert; A. Houben; M.F. Mette; Plant J. 2011) After DSB-induction by bleomycin during S and G2 phase and (E. Kapusi, L. Ma, C.H. Teo, G. Hensel, A. Himmelbach, Dept. of simultaneous labelling of chromosomes by incorporation of the Genebank (GB); I. Schubert, M.F. Mette, J. Kumlehn, A. Houben, base analogue ethynyldeoxyuridine, we found no indication for Chromosoma 2011) (Fig. 21, p. 54).

Fig. 26 Models for ‘break-induced replication’ were not confirmed by labelling of Vicia faba chromosomes with the base analogue ethynyldeoxyuridine in S and G2 after DSB induction; instead, extended conversion-like recombination repair was detected (I. Schubert, V. Schubert, J. Fuchs).

63 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Genome Size Evolution. To elucidate the mechanisms which Ko t s e r u b a , V., K. Pistrick, F.R. Bl a t t n e r , K. Ku m k e , O. We i s s , T. Ru t t e n , resulted in rapid shrinkage or expansion of the genomes of the J. Fu c h s , T. En d o , S. Na s u d a , A. Gh u k a s y a n & A. Ho u b e n : The carnivorous species Genlisea pygmaea (86 Mb) and G. hispidula evolution of the hexaploid grass Zingeria kochii (Mez) Tzvel. (>1500 Mb), respectively, de novo sequencing of genomes (2n=12) was accompanied by complex hybridization and and of transcriptomes of both species has been performed uniparental loss of ribosomal DNA. Mol. Phylogenet. Evol. 56 and assembly and annotation efforts have been started in (2010) 146-155. collaboration with G. Vu and A. Pecinka, MPIPZ, Köln (J. Fuchs, Ma, L., G.T. Vu, V. Sc h u b e r t , K. Wa t a n a b e , N. St e i n , A. Ho u b e n & I. H.X. Cao, GP, G. Jovtchev, I. Schubert, U. Scholz, T. Schmutzer, F. Sc h u b e r t : Synteny between Brachypodium distachyon and Bull, research group Bioinformatics and Information Technology Hordeum vulgare as revealed by FISH. Chromosome Res. 18 (BIT). (2010) 841-850. Pr e u t e n , T., E. Ci n c u , J. Fu c h s , R. Zo s c h k e , K. Li e r e & T. Bö r n e r : Fewer genes than organelles: extremely low and variable gene Publications copy numbers in mitochondria of somatic plant cells. Plant J. 64 (2010) 948-959. Peer Reviewed Papers Sa n e i , M., R. Pickering, J. Fu c h s , A.M. Ba n a e i Mo g h a d d a m , A. Dziurlikowska & A. Ho u b e n : Interspecific hybrids of Hordeum 2010 marinum ssp. marinum × H. bulbosum are mitotically stable Ba n a e i Mo g h a d d a m , A.M., J. Fu c h s , T. Cz a u d e r n a , A. Ho u b e n & M.F. and reveal no gross alterations in chromatin properties. Me t t e : Intraspecific hybrids of Arabidopsis thaliana revealed Cytogenet. Genome Res. 129 (2010) 110-116. no gross alterations in endopolyploidy, DNA methylation, Sc h m i d t -Le b u h n , A.N., J. Fu c h s , D. He r t e l , H. Hi r s c h , J. To i v o n e n & M. histone modifications and transcript levels. Theor. Appl. Ke s s l e r : An Andean radiation: Polyploidy in the tree genus Genet. 120 (2010) 215-226. Polylepis (Rosaceae, Sanguisorbeae). Plant Biol. 12 (2010) Be r r , A., E.J. McCa l l u m , R. Me n a r d , D. Me y e r , J. Fu c h s , A.W. Do n g & 917–926. W.H. Sh e n : Arabidopsis SET DOMAIN GROUP2 is required for Sreenivasulu , N., V. Ra d c h u k , A. Al a w a d y , L. Bo r i s j u k , D. We i e r , N. H3K4 trimethylation and is crucial for both sporophyte and St a r o s k e , J. Fu c h s , O. Mi e r s c h , M. St r i c k e r t , B. Us a d e l , U. Wo b u s , gametophyte development. Plant Cell 22 (2010) 3232-3248. B. Gr i m m , H. We b e r & W. We s c h k e : De-regulation of abscisic acid Du b i n , M., J. Fu c h s , R. Gr ä f , I. Sc h u b e r t & W. Ne l l e n : Dynamics of contents causes abnormal endosperm development in the a novel centromeric histone variant CENH3 reveals the barley mutant seg8. Plant J. 64 (2010) 589-603. evolutionary ancestral timing of centromere biogenesis. Ta k a h a s h i , N., M. Qu i m b a y a , V. Sc h u b e r t , T. La m m e n s , K. Va n d e p o e l e , Nucleic Acids Res. 38 (2010) 7526-7537. I. Sc h u b e r t , M. Ma t s u i , D. In z e , G. Be r x & L. De Ve y l d e r : The Hö f e r , M. & A. Me i s t e r : Genome size variation in Malus MCM-binding protein ETG1 aids sister chromatid cohesion species. J. Bot. 2010 (2010) Article ID 480873, 8 pages, required for postreplicative homologous recombination doi:10.1155/2010/480873. repair. PLoS Genet. 6 (2010) 41-54. Ho f f m a n , M.H., H. Sc h m u t h s , C. Ko c h , A. Me i s t e r & R.M. Fr i t s c h : Comparative analysis of growth, genome size, chromosome 2011 numbers and phylogeny of Arabidopsis thaliana and Be n n e r t , W., K. Ho r n , M. Ka u t h , J. Fu c h s , I. Ja k o b s e n , B. Øl l g a a r d , M. three cooccurring species of the Brassicaceae from Sc h n i t t l e r , M. St e i n b e r g & R. Vi a n e : Flow cytometry confirms Uzbekistan. J. Bot. 2010 (2010) Article ID 504613, 8 pages, reticulate evolution and reveals triploidy in Central European doi:10.1155/2010/504613. Diphasiastrum taxa (Lycopodiaceae, Lycophyta). Ann. Bot. Kinoshita, N., A. Be r r , C. Be l i n , R. Ch a p p u i s , N.K. Nishizawa & L. Lo p e z - 108 (2011) 867-876. Mo l i n a : Identification of growth insensitive to ABA3 (gia3), a Be n o r , S., J. Fu c h s & F.R. Bl a t t n e r : Genome size variation in recessive mutation affectingABA signaling for the control of Corchorus olitorius (Malvaceae s.l.) and its correlation with early post-germination growth in Arabidopsis thaliana. Plant elevation and phenotypic traits. Genome 54 (2011) 575- Cell Physiol. 51 (2010) 239-251. 585.

64 En k e , N., J. Fu c h s & B. Gemeinhölzer : Shrinking genomes? Evidence Sc h u b e r t , I. & P. Sh a w : Organization and dynamics of plant from genome size variation in Crepis (Compositae). Plant interphase chromosomes. Trends Plant Sci. 16 (2011) 273- Biol. 13 (2011) 185-193. 281. He c k m a n n , S., I. Le r m o n t o v a , B. Be r c k m a n s , L. De Ve y l d e r , H. Bä u m l e i n Sc h u b e r t , V.: No neocentric activity on Aegilops markgrafii & I. Sc h u b e r t : The E2F transcription factor family regulates chromosome E. Cytogenet. Genome Res. 132 (2011) 100- CENH3 expression in Arabidopsis thaliana. Plant J. 68 (2011) 104. 646-656. Sc h u b e r t , V., A. Me i s t e r , H. Ts u j i m o t o , T.R. En d o & A. Ho u b e n : Similar Jo v t c h e v , G., B.E. Bo r i s o v a , M. Ku h l m a n n , J. Fu c h s , K. Wa t a n a b e , I. Sc h u b e r t rye A and B chromosome organization in meristematic and & M.F. Me t t e : Pairing of lacO tandem repeats in Arabidopsis differentiated interphase nuclei.C hromosome Res. 19 (2011) thaliana nuclei requires the presence of hypermethylated, 645-655. large arrays at two chromosomal positions, but does not Sêdzielewska , K.A., J. Fu c h s , E.M. Te m s c h , K. Ba r o n i a n , R. Wa t z k e & G. depend on H3-lysine-9-dimethylation. Chromosoma 120 Ku n z e : Estimation of the Glomus intraradices nuclear DNA (2011) 609-619. content. New Phytol. 192 (2011) 794-797. Ka r i m i As h t i y a n i , R., A.M. Ba n a e i Mo g h a d d a m , V. Sc h u b e r t , T. Ru t t e n , Te o , C.H., L. Ma, E. Ka p u s i , G. He n s e l , J. Ku m l e h n , I. Sc h u b e r t , A. Ho u b e n J. Fu c h s , D. De m i d o v , F.R. Bl a t t n e r & A. Ho u b e n : AtHaspin & M.F. Me t t e : Induction of telomere-mediated chromosomal phosphorylates histone H3 at threonine 3 during mitosis truncation and stability of truncated chromosomes in and contributes to embryonic patterning in Arabidopsis. Arabidopsis thaliana. Plant J. 68 (2011) 28-39. Plant J. 68 (2011) 443-454. Wi e s e , C., T. Ni k o l o v a , I. Za h a n i c h , S. Su l z b a c h e r , J. Fu c h s , S. Ya m a n a k a , Le r m o n t o v a , I., O. Ko r o l e v a , T. Ru t t e n , J. Fu c h s , V. Sc h u b e r t , I. Mo r a e s , E. Gr a f , U. Ra v e n s , K.R. Bo h e l e r & A.M. Wo b u s : Differentiation D. Kö s z e g i & I. Sc h u b e r t : Knockdown of CENH3 in Arabidopsis induction of mouse embryonic stem cells into sinus node- reduces mitotic divisions and causes sterility by disturbed like cells by suramin. Int. J. Cardiol. 147 (2011) 95-111. meiotic chromosome segregation. Plant J. 68 (2011) 40-50. Za k r z e w s k i , F., B. We i s s h a a r , J. Fu c h s , E. Ba n n a c k , A.E. Mi n o c h e , J.C. Le r m o n t o v a , I., T. Ru t t e n & I. Sc h u b e r t : Deposition, turnover, and Do h m , H. Hi m m e l b a u e r & T. Sc h m i d t : Epigenetic profiling of release of CENH3 at Arabidopsis centromeres. Chromosoma heterochromatic satellite DNA. Chromosoma 120 (2011) 120 (2011) 633-640. 409-422. Ma r q u e s , A., J. Fu c h s , L. Ma, S. He c k m a n n , M. Gu e r r a & A. Ho u b e n : Characterization of eu- and heterochromatin of Citrus with a focus on the condensation behavior of 45S rDNA chromatin. PhD and Diploma Theses Cytogenet. Genome Res. 134 (2011) 72-82. 2010 Mo r a e s , I.C., I. Le r m o n t o v a & I. Sc h u b e r t : Recognition of A. thaliana We i s s l e d e r , A.: Selection and characterization of Arabidopsis centromeres by heterologous CENH3 requires high similarity thaliana cohesin and condensin T-DNA insertion mutants. to the endogenous protein. Plant Mol. Biol. 75 (2011) 253- (PhD Thesis) Martin-Luther-Universität Halle-Wittenberg, 261. Naturwissenschaftliche Fakultät I, Institut für Biologie, Sc h u b e r t , I.: ‘Sex and crime’ in evolution – why sexuality was so Halle/S. (2010) 90 pp. successful. Genes Genet. Syst. 86 (2011) 1-6. Sc h u b e r t , I.: Between genes and genomes – future challenges 2011 for cytogenetics. Front. Genet. 2 (2011) 30. Mo r a e s , I.: Structural requirements for CENH3 targeting to Sc h u b e r t , I. & M.A. Ly s a k : Interpretation of karyotype evolution centromeric chromatin. (PhD Thesis) Martin-Luther-Universität should consider chromosome structural constraints. Trends Halle-Wittenberg, Naturwissenschaftliche Fakultät I, Institut für Genet. 27 (2011) 207-216. Biologie, Bereich Pflanzenphysiologie, Halle/S. (2011) 82 pp. Sc h u b e r t , I., V. Sc h u b e r t & J. Fu c h s : No evidence for ‘break-induced replication’ in a higher plant – but break-induced conversion may occur. Front. Plant Sci. 2 (2011) 8.

65 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Research Group: Chromosome Structure and Function Head: Dr. Andreas Houben

Scientists by capturing of additional A-derived and organellar sequences, and the amplification of repeats, which are important for the IPK financed specific drive for B chromosome maintenance. The most unex- Heckmann, Stefan (0.50) pected result of our analysis is the discovery that B chromo- Karimi Ashtiyani, Raheleh (Pakt für Forschung und Innovation, somes are rich in gene fragments that represent copies of 01.09.2010-31.01.2011) A chromosome genes (A. Banaei-Moghaddam, S. Klemme, in Ma, Lu, Dr. (0.50 Pakt für Forschung und Innovation, cooperation with the research groups Experimental Taxonomy, till 31.10.2011; 0.50, 01.11.-31.12.2011) Bioinformatics and Information Technology, Genome Diversity and external cooperation partners). Grant Positions Banaei Moghaddam, Ali Mohammad (DFG) Engineered minichromosomes offer an enormous opportu- Demidov, Dmitri, Dr. (DFG / SFB 648) nity to plant biotechnology as they have the potential to simul- Karimi Ashtiyani, Raheleh (Saxony-Anhalt, till 31.08.2010; taneously transfer and stably express multiple genes. Following 0.50 Overhead, 11.04.-31.07.2011; BMBF, since 01.08.2011) a top-down approach, we truncated endogenous chromo- Klemme, Sonja (0.50 DFG, since 01.03.2010) somes in barley (Hordeum vulgare) and in Arabidopsis by Sanei, Maryam (0.50 DFG, till 30.09.2010; 0.50 Overhead, Agrobacterium-mediated transfer of T-DNA constructs contain- 01.10.-31.12.2010) ing telomere sequences. Truncated chromosomes were transmissible in sexual reproduction, but were inherited at rates Visiting Scientists/Scholars lower than expected according to Mendelian rules (L. Ma, in Acevedo-Garcia, Johanna (self-financed, 31.07.-13.08.2011) cooperation with the research groups Structural Cell Biology; Agueci, Francesco (self-financed, 01.01.-30.06.2010) Karyotype Evolution and Plant Reproductive Biology – Depart- Caillieux, Erwann (DAAD, 10.10.-28.10.2011) ment of Physiology and Cell Biology). Cuacos, Maria (University of Madrid/Ministry of Education in Spain, 01.06.-31.08.2011) Precise segregation of chromosomes during cell divisions is Evtushenko, Elena, Dr. (DFG, 01.09.-29.11.2010) essential for genetic stability. We demonstrated that Aurora Gonzalez Garcia, Miriam (University of Madrid/Ministry of kinases of plants are involved in the regulation of the spin- Education in Spain, 02.05.-02.08.2010) dle assembly checkpoint (SAC) pathway. The SAC proteins Ishii, Takayoshi (self-financed, 17.10.-30.10.2011) AtMad2 and AtBub1-R1 interact with AtAurora1 in vivo and Kaur, Gurmeet (DAAD, 17.10.-01.12.2010) serve as kinase substrates for AtAurora1 in vitro. Deregula- Sanei, Maryam (self-financed, 01.07.-01.10.2011) tion of SAC expression yields poly- and/or aneuploids (D. Sousa, Aretuza (Ludwig Maximilians University Munich, Demidov, in cooperation with the research group Karyotype 28.02.-11.03.2011) Evolution and external cooperation partners). The cell cycle-specific phosphorylation of histone H3 at threonine 3 is highly conserved, but the significance Goals of Haspin kinase driven H3 phosphorylation for plant development is unclear. In Arabidopsis, AtHaspin contributes Analysis and manipulation of structure and regulation of plant to embryonic patterning and altered expression of AtHaspin chromosomes. induced pleiotropic phenotypes. Further, a cross-talk exists between phosphorylation of H3T3 by AtHaspin and other post-translational modifications. The level of H3T3 Research Report phosphorylation activity of recombinant AtHaspin is reduced by methylation or acetylation of H3K4 and by phosphorylation Supernumerary B chromosomes occur in all eukaryotic phyla at H3T6. Modifications at H3K9, H3S10 and H3T11 had no and are assumed to represent a specific type of selfish DNA. Lit- influence on the activity of AtHaspin (R. Karimi Ashtiyani). tle is known about their origin and molecular composition. In cooperation with several cooperation partners we determined the sequence composition of rye B chromosomes. Based on a comparative sequence analysis of the A and B chromosomes of rye, we propose the first comprehensive model of B chromosome evolution (see Fig. 27, p. 67). This model includes the identification of the origin of B chromosomes by the recombination of A chromosome segments. This process was followed

66 Fig. 27 Model on the stepwise evolution of the rye B chromosome. (1) Reciprocal translocation between 3R and 7R and subsequent imbalanced segregation of the small translocation product results in duplication of fragments of A chromosomes 3R and 7R (2) which do not pair to the homologous A chromosome regions and represent a proto-B. (3) Accumulation of organellar and A chromosome-derived DNA fragments, amplification of B-specific repeats, erosion and inactivation of A-derived genes (Muller’s ratchet) as well as gain of a ‘chromosome drive’ result in a B chromosome (M. Martis, S. Klemme, A.M. Banaei Moghaddam, F. R. Blattner, J. Macas, T. Schmut- zer, U. Scholz, H. Gundlach, T. Wicker, H. Šimková, P. Novák, P. Neumann, M. Kubaláková, E. Bauer, G. Haseneyer, J. Fuchs, J. Doležel, N. Stein, K.F.X. Mayer and A. Houben).

The chromatin features of generative and vegetative nuclei Publications after first and second pollen mitosis in rye (Secale cereale) were analysed. The condensed chromatin of generative nuclei Peer Reviewed Papers is earmarked by an enhanced level of histone H3K4 and/or K9 dimethylation and H3K9 acetylation. The less condensed 2010 vegetative nuclei are RNA polymerase II-positive. In rye, unlike Ba n a e i Mo g h a d d a m , A.M., J. Fu c h s , T. Cz a u d e r n a , A. Ho u b e n & M.F. in Arabidopsis, CENH3 is not excluded from the chromatin of the Me t t e : Intraspecific hybrids of Arabidopsis thaliana revealed vegetative nucleus and the condensation degree of centromeric no gross alterations in endopolyploidy, DNA methylation, and subtelomeric regions did not differ between generative histone modifications and transcript levels. Theor. Appl. and vegetative nuclei. Differences between rye andArabidopsis Genet. 120 (2010) 215-226. suggest that the chromatin organisation in mature nuclei of Ka c z m a r c z y k , A., A. Ho u b e n , E.R.J. Ke l l e r & M.F. Me t t e : Influence pollen grains is not universal across angiosperms (A. Houben, of cryopreservation on the epigenetic state of potato. in cooperation with external cooperation partners). CryoLetters 31 (2010) 380-391. Ko t s e r u b a , V., K. Pistrick, F.R. Bl a t t n e r , K. Ku m k e , O. We i s s , T. Ru t t e n , The structure of holocentric chromosomes was analysed J. Fu c h s , T. En d o , S. Na s u d a , A. Gh u k a s y a n & A. Ho u b e n : The in mitotic cells of Luzula elegans. Our studies challenged the evolution of the hexaploid grass Zingeria kochii (Mez) Tzvel. notion of a ‘diffuse’ centromere organisation along holocentric (2n=12) was accompanied by complex hybridization and chromosomes. Instead, our observations provided evidence for uniparental loss of ribosomal DNA. Mol. Phylogenet. Evol. 56 a longitudinal CENH3-postive, centromere-like groove along (2010) 146-155. each sister chromatid. We have first evidence that a holocentric Lo l a s , I.B., K. Hi m a n e n , J.T. Gr ø n l u n d , C. Ly n g g a a r d , A. Ho u b e n , centromere organisation exists throughout all stages of meiosis M. Me l z e r , M. Va n Lijsebettens & K.D. Gr a s s e r : The transcript in L. elegans. From a cytological point of view, in Luzula the elongation factor FACT affects Arabidopsis vegetative and sequence of meiotic events seems to be inverted. Apparently, reproductive development and genetically interacts with the first division is equational whereas the second division HUB1/2. Plant J. 61 (2010) 686-697. is reductional (S. Heckmann, in cooperation with external Ma, L., G.T. Vu, V. Sc h u b e r t , K. Wa t a n a b e , N. St e i n , A. Ho u b e n & I. cooperation partners). Sc h u b e r t : Synteny between Brachypodium distachyon and Hordeum vulgare as revealed by FISH. Chromosome Res. 18 (2010) 841-850. Ne l i s s e n , H., S. De Gr o e v e , D. Fl e u r y , P. Ne y t , L. Br u n o , M.B. Bi t o n t i , F. Vandenbussche , D. Va n De r St r a e t e n , T. Ya m a g u c h i , H. Ts u k a y a , E. Wi t t e r s , G. De Ja e g e r , A. Ho u b e n & M. Va n Lijsebettens : Plant elongator regulates auxin-related genes during RNA polymerase II transcription elongation. Proc. Natl. Acad. Sci. U.S.A. 107 (2010) 1678-1683.

67 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Sa n e i , M., R. Pickering, J. Fu c h s , A.M. Ba n a e i Mo g h a d d a m , A. Pe d e r s e n , D.S., F. Co p p e n s , L. Ma, M. An t o s c h , B. Ma r k t l , T. Me r k l e , G.T. Dziurlikowska & A. Ho u b e n : Interspecific hybrids of Hordeum Be e m s t e r , A. Ho u b e n & K.D. Gr a s s e r : The plant-specific family of marinum ssp. marinum × H. bulbosum are mitotically stable DNA-binding proteins containing three HMG-box domains and reveal no gross alterations in chromatin properties. interacts with mitotic and meiotic chromosomes. New Cytogenet. Genome Res. 129 (2010) 110-116. Phytol. 192 (2011) 577-589. Sa n e i , M., R. Pickering, K. Ku m k e , S. Na s u d a & A. Ho u b e n : Loss of 2011 centromeric histone H3 (CENH3) from centromeres precedes Ar e f i a n , E., J. Ki a n i , M. So l e i m a n i , S.A. Sh a r i a t i , S.H. Ag h a e e -Ba k h t i a r i , uniparental chromosome elimination in interspecific barley A. At a s h i , Y. Gh e i s a r i , N. Ah m a d b e i g i , A.M. Ba n a e i -Mo g h a d d a m , hybrids. (This article has been selected and evaluated by M. Na d e r i , N. Na m v a r a s l , L. Go o d & O.R. Fa r i d a n i : Analysis of Jiming Jiang, a Member of the Faculty of 1000 (F1000), which microRNA signatures using size-coded ligation-mediated places this work in that library of the top 2 % of published PCR. Nucleic Acids Res. 39 (2011) e80. articles in biology and medicine). Proc. Natl. Acad. Sci. U.S.A. Ba n a e i Mo g h a d d a m , A.M., F. Ro u d i e r , M. Se i f e r t , C. Be r a r d , M.L. 108 (2011) E498-E505. Ma g n i e t t e , R. Ka r i m i As h t i y a n i , A. Ho u b e n , V. Co l o t & M.F. Sc h u b e r t , V., A. Me i s t e r , H. Ts u j i m o t o , T.R. En d o & A. Ho u b e n : Similar Me t t e : Additive inheritance of histone modifications in rye A and B chromosome organization in meristematic and Arabidopsis thaliana intra-specific hybrids. Plant J. 67 differentiated interphase nuclei.C hromosome Res. 19 (2011) (2011) 691-700. 645-655. Bandopadhyay , R., S. Ru s t g i , R.K. Ch a u d h u r i , P. Kh u r a n a , J.P. Te o , C.H., L. Ma, E. Ka p u s i , G. He n s e l , J. Ku m l e h n , I. Sc h u b e r t , A. Ho u b e n Kh u r a n a , A.K. Ty a g i , H.S. Ba l y a n , A. Ho u b e n & P.K. Gu p t a : Use of & M.F. Me t t e : Induction of telomere-mediated chromosomal methylation filtration and C(0)t fractionation for analysis of truncation and stability of truncated chromosomes in genome composition and comparative genomics in bread Arabidopsis thaliana. Plant J. 68 (2011) 28-39. wheat. J. Genet. Genomics 38 (2011) 315-325. v a n Da m m e , D., B. d e Ry b e l , G. Gu d e s b l a t , D. De m i d o v , W. Gr u n e w a l d , He c k m a n n , S., I. Le r m o n t o v a , B. Be r c k m a n s , L. De Ve y l d e r , H. Bä u m l e i n T. d e Sm e t , A. Ho u b e n , T. Be e c k m a n & E. Ru s s i n o v a : Arabidopsis & I. Sc h u b e r t : The E2F transcription factor family regulates α Aurora kinases function in formative cell division plane CENH3 expression in Arabidopsis thaliana. Plant J. 68 (2011) orientation. Plant Cell 23 (2011) 4013-4024. 646-656. He c k m a n n , S., E. Sc h r o e d e r -Re i t e r , K. Ku m k e , L. Ma, K. Na g a k i , M. Mu r a t a , G. Wa n n e r & A. Ho u b e n : Holocentric chromosomes of Luzula elegans are characterized by a longitudinal PhD and Diploma Theses centromere groove, chromosome bending, and a terminal 2010 nucleolus organizer region. Cytogenet. Genome Res. 134 Ag u e c i , F.: Characterization of NIMA-like kinases in Arabidopsis (2011) 220-8. thaliana. (PhD Thesis) Martin-Luther-Universität Halle- Ho u b e n , A., K. Ku m k e , K. Na g a k i & G. Ha u s e : CENH3 distribution Wittenberg, Naturwissenschaftliche Fakultät III, Halle/S. and differential chromatin modifications during pollen (2010) 129 pp. development in rye (Secale cereale L.). Chromosome Res. 19 Ba n a e i Mo g h a d d a m , A.M.: Dynamics of chromatin modifications (2011) 471-480. and other nuclear features in response to intraspecific Ho u b e n , A., S. Na s u d a & T.R. En d o : Plant B chromosomes. Methods hybridization in Arabidopsis thaliana. (PhD Thesis) Martin- Mol. Biol. 701 (2011) 97-111. Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Ho u b e n , A., M. Sa n e i & P. Pickering: Barley doubled-haploid Fakultät I, Halle/S. (2010) 143 pp. production by uniparental chromosome elimination. Plant Ho u b e n , A.: Funktion und Evolution pflanzlicherB -Chromosomen. Cell Tiss. Organ Cult. 104 (2011) 321-327. (Habilitation) Martin-Luther-Universität Halle-Wittenberg, Ka r i m i As h t i y a n i , R., A.M. Ba n a e i Mo g h a d d a m , V. Sc h u b e r t , T. Ru t t e n , Halle/S. (2010) 57 pp. J. Fu c h s , D. De m i d o v , F.R. Bl a t t n e r & A. Ho u b e n : AtHaspin phosphorylates histone H3 at threonine 3 during mitosis 2011 and contributes to embryonic patterning in Arabidopsis. Sa n e i , M.: Analysis of uniparental chromosome elimination in Plant J. 68 (2011) 443-454. wide crosses. (PhD Thesis) Martin-Luther-Universität Halle- Ma r q u e s , A., J. Fu c h s , L. Ma, S. He c k m a n n , M. Gu e r r a & A. Ho u b e n : Wittenberg, Halle/S. (2011) 134 pp. Characterization of eu- and heterochromatin of Citrus with a focus on the condensation behavior of 45S rDNA chromatin. Cytogenet. Genome Res. 134 (2011) 72-82. Additional Publications 2009 Ok a d a , T., K. It o , S.D. Jo h n s o n , K. Oe l k e r s , G. Su z u k i , A. Ho u b e n , Y. Mu- Ka r i m i As h t i y a n i , R.: AtHaspin, a putative histone H3-specific ki- k a i & A.M. Ko l t u n o w : Chromosomes carrying meiotic avoid- nase in Arabidopsis thaliana? (PhD Thesis) Martin-Luther-Uni- ance loci in three apomictic eudicot Hieracium subgenus Pi- versität Halle-Wittenberg, Naturwissenschaftliche Fakultät I, losella species share structural features with two monocot Halle/S. (2009) 113 pp. apomicts. Plant Physiol. 157 (2011) 1327-1341.

68 Research Group: Apomixis Head: Dr. Timothy F. Sharbel

Scientists lysed. A flow cytometric analysis of 96 seeds per each accession demonstrates genotype-specific quantitative variation for sex- IPK financed ual and apomictic seed production. These data demonstrate Corral García, José María, Dr. (Pakt für Forschung und (1) that H. perforatum colonised North America from three Innovation, till 28.02.2010; 0.75 / 1.00, 01.03.2010-31.12.2011) distinct European genetic lineages over the last 160 years, and Mau, Martin (0.50) (2) that invasiveness of this species has been characterised by an increased frequency of apomictic seed production in North Grant Positions America. We have sequenced the complete flower transcrip- Aliyu, Olawale Mashood, Dr. (DFG, since 01.04.2010) tomes (normalised cDNA) of two sexual and two apomic- Otto, Lars-Gernot, Dr. (0.50 BMBF, 15.02.-14.08.2011) tic accessions using 454 FLX technology (at the IPK). These Pellino, Marco (0.50 DFG, since 11.01.2010) data are being mined for SNPs to test hypotheses of sexual and Puente Molins, Marta, Dr. (DFG, till 14.04.2011; 0.50 Overhead, asexual genome evolution (G. Galla). 15.04.-14.06.2011) The Boechera holboellii complex: Using our high-throughput Visiting Scientists/Scholars seed screening method, we have analysed over 24,000 Amiteye, Samuel (International Max Planck Research School single seeds in 76 Boechera accessions, and have shown grant) genotype-specific quantitative variation for apomeiosis, Dobes, Christoph, Dr. (FWF, 01.08.-08.08.2010) parthenogenesis and pseudogamy. Besides the production Eradasappa, E. (Fellowship World Bank, since 14.10.2011) of unreduced egg cells (apomeiosis), apomictic Boechera also Galla, Giulio (self-financed, 08.03.-12.04.2010 and 05.08.-26.08.2011) produce high frequencies of unreduced pollen for balanced Geetha, K. A. (Fellowship World Bank, 02.05.-31.07.2011) endosperm formation. Custom-made high-density microarrays Hojsgaard, Diego, Dr. (FWF, 17.10.-23.10.2010; 23.03.-31.05.2011) were built using sequences generated from flower-specific Jordon-Thadeus, Ingrid (DFG, 28.02.-06.03.2010) normalised cDNA libraries which were sequenced using Koperdakova, Jana (EU COST Action, 01.09.-19.09.2011) 454 technology, and our analyses have enabled us to design Lovell, John Thomsen (DFG Fellowship, 25.09.-05.12.2011) targeted experiments which have led to the identification of a Majeský, Lúbos (University of Olomouc, 12.09.-12.12.2011) single gene each underlying unreduced egg (J. Corral) and Morillas, Javier (University of Granada, 21.02.-18.04.2011) pollen (M. Mau) formation. Otto, Lars-Gernot, Dr. (self-financed, till 31.07.2010; 1.08.2010-14.02.2011; since 15.08.2011) Our continuing work on the candidate apomeiosis gene has Piwczynski, Marcin (Nicolaus Copernicus University, Poland, led to the identification of a single apomixis-specific allele 30.08.-27.09.2010) shared by all apomicts, regardless of different genetic, ploidy or Puente Molins, Marta, Dr. (self-financed, since 15.06.2011) geographic backgrounds. Verification of this apomixis-specific Rois, Ana Sofia (Instituto Superior de Agronomia, allele has been performed using many hundreds of qRT-PCR 24.01.-04.02.2011) analyses, which demonstrate that the apomixis allele is Scheffknecht, Susanne (FWF, 01.08.-14.08.2010) exclusively expressed in apomictic ovules. Scheriau, Charlotte (DFG, 28.02.-06.03.2010) Thiel, Thomas (International Max Planck Research School grant) We have designed a 3 x 750,000 feature custom (NimbleGen) Valverde, Francisco (University of Granada, 21.02.-18.04.2011) comparative genome hybridisation (CGH) microarray based Weber, Anna (EU COST Action, 14.08.-10.09.2011) upon the sequenced cDNA libraries, and have completed a copy number variation (CNV) experiment on 10 different genotypes each of sexual and apomictic Boechera genotypes. Goals The data demonstrate (1) that apomictic and sexual genomes are characterised by significant numbers of duplications Genomic and transcriptomic analyses to identify candidate and deletions respectively, and (2) that retroelements apomixis factors in wild monocot and dicot species. have accumulated in a clock-like pattern in apomictic genomes, as would be expected by evolutionary theory (O. Aliyu, M. Seifert). We have furthermore cloned, sequenced and Research Report validated both known and novel miRNAs from sexual and apomictic Boechera, and are presently examining their effect Hypericum perforatum: We have chosen 650 accessions, rep- on differential gene regulation in microdissected ovules (see resenting different ploidies and worldwide geographic origins, Fig. 28, p. 70; S. Amiteye). for which data from 30 microsatellite markers have been ana-

69 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Fig. 28 Abundance (based on total number of transcripts) of previously-identified plant-miRNA families in sexual and apomictic Boechera (S. Amiteye).

The Ranunculus auricomus complex: Using Illumina tech- Am i t e y e , S., J.M. Co r r a l , H. Vo g e l & T.F. Sh a r b e l : Analysis of conserved nology, we have sequenced the complete transcriptomes microRNAs in floral tissues of sexual and apomicticBoechera of two apomictic and three sexual Ranunculus genotypes, species. BMC Genomics 12 (2011) 500. and are using this data to design a 3 x 1.4 million feature cus- Br i n g e z u , T.G.G., T.F. Sh a r b e l & W.E. We b e r : Grain development and tom (NimbleGen) expression microarray. Live ovules from endoreduplication in maize and the impact of heat stress. 4 developmental stages and 5 accessions each of sexual and Euphytica 182 (2011) 363-376. apomictic Ranunculus have been microdissected for a compar- Co r r a l , J.M., M.P. Mo l i n s , O.M. Al i y u & T.F. Sh a r b e l : Isolation and ative transcriptome profiling experiment. Over 100,000 high characterization of microsatellite loci from apomictic quality SNPs have been mined from the sequence data, Hypericum perforatum (Hypericaceae). Am. J. Bot. 98 (2011) and these are being analysed in order to test Muller’s Ratchet, e167-e169. the hypothesised mutation accumulation that should occur in Ga l l a , G., G. Ba r c a c c i a , A. Sc h a l l a u , M. Pu e n t e Mo l i n s , H. Bä u m l e i n asexual genomes (M. Pellino, T. Schmutzer). & T.F. Sh a r b e l : The cytohistological basis of apospory in Hypericum perforatum L. Sex. Plant Reprod. 24 (2011) 47-61. Ga l l a , G., S. Ze n o i , G. Ma r c o n i , G. Ma r i n o , A. Bo t t o n , F. Pi n o s a , S. Citterio, Publications S. Co l l a n i , B. Ru p e r t i , K. Pa l m e , E. Al b e r t i n i , M. Pe z z o t t i , H. d e Jo n g , M. Ma u , T.F. Sh a r b e l , N. d e So r m e , D. Ge e l e n & G. Ba r c a c c i a : Sporophytic Peer Reviewed Papers and gametophytic functions of the cell cycle-associated Mob1 gene in Arabidopsis thaliana L. Gene 484 (2011) 1-12. 2010 Mu ñ o z -Pa j a r e s , A.J., M.B. He r r a d o r , M. Ab d e l a z i z , F.X. Pi c ó , T.F. Sh a r b e l , Al i y u , O.M., M.E. Sc h r a n z & T.F. Sh a r b e l : Quantitative variation for J.M. Gó m e z & F. Pe r f e c t t i : Characterization of microsatellite apomixis components in the genus Boechera. Am. J. Bot. 97 loci in Erysimum mediohispanicum (Brassicaceae) and cross- (2010) 1719-1731. amplification in related species. Am. J. Bot. 98 (2011) e287-289. Am b r o s i , D., G. Ga l l a , M. Pu r e l l i , T. Ba r b i , A. Fa b b r i , S. Lu c r e t t i , T.F. Pa u l e , J., T.F. Sh a r b e l & C. Do b e s : Apomictic and sexual lineages Sh a r b e l & G. Ba r c a c c i a : DNA Markers and FCSS analyses shed of the Potentilla argentea L. group (Rosaceae): Cytotype and light on the genetic diversity and reproductive strategy of molecular genetic differentiation.T axon 60 (2011) 721-732. Jatropha curcas L. Diversity 2 (2010) 810-836. Pe l l i n o , M., T.F. Sh a r b e l , M. Ma u , S. Am i t e y e & J.M. Co r r a l : Selection Sh a r b e l , T.F., M.L. Vo i g t , J.M. Co r r a l , G. Ga l l a , J. Ku m l e h n , C. Kl u k a s , F. of reference genes for quantitative real-time PCR expression Sc h r e i b e r , H. Vo g e l & B. Ro t t e r : Apomictic and sexual ovules of studies of microdissected reproductive tissues in apomictic Boechera display heterochronic global gene expression patterns and sexual Boechera. BMC Res. Notes 4 (2011) 303. (Hofmann, N.R.: Apomixis and gene expression in Boechera. Plant Cell 22 (2010) 539. Highlight of Sharbel et al. 2010 by Plant Cell scientific editor). Plant Cell 22 (2010) 655-671. Books and Book Chapters

2011 2010 Am i t e y e , S., J.M. Co r r a l & T.F. Sh a r b e l : Overview of the potential Al b e r t i n i , E., G. Ba r c a c c i a , A. Ma z z u c a t o , T.F. Sh a r b e l & M. Fa l c i n e l l i : of microRNAs and their target gene detection for cassava Apomixis in the era of biotechnology. In: Pu a , E.-C. & M.R. Da v - (Manihot esculenta) improvement. Afr. J. Biotechnol. 10 e y (Eds.): Plant Developmental Biology ‑ Biotechnological Per- (2011) 2562-2573. spectives, Vol. 1, Springer, Berlin-Heidelberg (2010) 405-436.

70 Research Group: Genome Plasticity Head: Dr. Renate Schmidt

Scientists Research Report

IPK financed Genetic analysis of seed and yield traits in Arabidopsis Le, Loan Thanh (0.50, since 05.01.2011) thaliana. We used digital image analysis to evaluate reciprocal recombinant inbred lines (RILs) with respect to seed length, Grant Positions width and area. Other traits related to seed yield were also Boudichevskaia, Anastassia, Dr. (BMBF, till 30.09.2011; Saxony- assessed, such as 100-seed weight, seed weight per plant, silique Anhalt, 01.10.-31.10.2011; 0.50 Overhead, 01.11.-31.12.2011) length and number of seeds per silique. Transgression beyond Cao, Hieu Xuan, Dr. (BMBF, since 28.06.2010) the parental values was observed for all traits. Quantitative trait Koppolu, Jahnavi (0.50 Saxony-Anhalt, since 01.05.2010) locus (QTL) mapping revealed genomic regions of importance Voigtländer, Susan (0.50 Saxony-Anhalt, till 31.08.2010) for all characters of interest (A. Boudichevskaia, J. Koppolu, S. Voigtländer, in collaboration with T. Altmann, research group Visiting Scientists/Scholars Heterosis) (Fig. 29). The same traits are also evaluated in a broad Le, Phuong Dung (MOET/DAAD, since 24.09.2011) set of Arabidopsis thaliana natural accessions to serve as a basis for association analyses (J. Koppolu). In order to find the genes underlying the seed traits, whole- Goals genome expression profiles of seeds at one particular time point during seed development were established for >100 Study and exploitation of naturally occurring genetic variation recombinant inbred lines. We are currently analysing which in Brassicaceae. genes show expression QTL (eQTL) and whether some of these eQTL may colocalise with the QTL that were identified for the seed traits (A. Boudichevskaia).

Fig. 29 The upper panel shows seed area and seed weight variation in reciprocal populations of recombinant inbred lines. The lower panel on the left presents correlations between seed area and seed weight in the different RILs. At the right the LOD scores for several seed traits are plotted along Arabidopsis thaliana chromosome 1 to reveal QTL (A. Boudichevskaia and J. Koppolu).

71 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Natural variation of genes involved in post-transcriptional After confirming that the multidimensional screening platform gene silencing (PTGS). The study of mutants with impaired was suitable for PCR-based screening it was evaluated whether PTGS led to the discovery of many genes that play important screening in multiplex format is also feasible. Single nucleotide roles in this process. However, little is known about the genetic polymorphisms (SNPs) can be used in highly multiplexed variability of these loci. To address this point we have selected fashion therefore gene-specific SNP assays were designed and genetically diverse Arabidopsis thaliana accessions and we are used for the screening of all BAC clone pools with an Illumina analysing genes involved in PTGS with respect to sequence Veracode® system. The results of the screening revealed that the variation. Alleles for functional tests are selected based on the multidimensional screening platform can be used successfully results of the comparative sequence analysis. for multiplex PCR assays. The design of gene-specific SNP assays Molecular genetic analysis of transgene expression in the A. for the allopolyploid Brassica napus genome is hampered by thaliana accession Columbia-0 revealed that PTGS is triggered high sequence identities between homoeologous genes. if the transcript level surpasses a gene-specific threshold. However, the high frequency of SNPs between homoeologous Importantly, the onset and/or the spread of silencing can be oilseed rape genes (“intergenomic SNPs“) may represent an reliably monitored in populations of transgenic lines carrying interesting alternative source for a BAC screening platform of multiple copies of a GFP transgene under the control of the this species. We are therefore particularly interested to study CaMV 35S promoter. In order to study the functional relevance now whether intergenomic SNPs are suitable for identification of selected sequence variants we are introgressing the alleles and differentiation of homoeologous genes (H.X. Cao). to be tested into the Columbia-0 lines carrying GFP transgenes. The onset and spread of silencing of the GFP genes will then be assessed in the different introgression lines and compared to the pattern observed in the Columbia-0 genetic background Publications (L.T. Le, D.P. Le). Books and Book Chapters PCR-based screening platform for an oilseed rape BAC library. A Brassica napus BAC library consisting of more than 2011 80,000 clones was used for this work. The 216 microtitre plates Sc h m i d t , R. & I. Ba n c r o f t : Perspectives on genetics and genomics that contain all BAC clones of the library were conceptually of the Brassicaceae. In: Sc h m i d t , R. & I. Ba n c r o f t (Eds.): Genetics arranged in a cube consisting of 36 layers, 48 columns, and 48 and Genomics of the Brassicaceae. Plant Genetics and rows. The clones were pooled according to their position in Genomics: Crops and Models, Vol. 9, Springer, New York- the cube along the six distinct coordinate axes to yield a total Dordrecht-Heidelberg-London (2011) 617-632. of 276 clone pools. Using this scheme, each of the clones is Sc h m i d t , R. & I. Ba n c r o f t (Eds.): Genetics and Genomics of the represented only once in each of the six dimensions. Screening Brassicaceae. Plant Genetics and Genomics: Crops and of the six-dimensional clone pools for a particular locus usually Models, Vol. 9, Springer, New York-Dordrecht-Heidelberg- does not allow for the unambiguous deconvolution of all clone London (2011) 677 pp. coordinates that may harbour this locus because the BAC To w n , C., R. Sc h m i d t & I. Ba n c r o f t : Comparative genome analysis library encompasses between 8 and 10 genome equivalents. at the sequence level in the Brassicaceae. In: Sc h m i d t , R. & I. In order to improve the deconvolution of the BAC coordinates, Ba n c r o f t (Eds.): Genetics and Genomics of the Brassicaceae. additional sets of clone pools were generated. Plant Genetics and Genomics: Crops and Models, Vol. 9, Springer, New York-Dordrecht-Heidelberg-London (2011) 171-194.

72 Research Group: Epigenetics Head: Dr. Michael Florian Mette

Scientists Beside DNA methylation, histone modifications play a central role in the regulation of gene activity in plants. To check the IPK financed role of histone methylation in RdDM in A. thaliana, DNA Finke, Andreas (0.50) methylation and transcript formation at AtSN1, an endogenous Teo, Chee How, Dr. (Pakt für Forschung und Innovation) target of RdDM, were tested in plants carrying mutations in two SET-domain histone methyltransferase genes. Indeed, Grant Positions DNA methylation was reduced and transcript accumulation Borisova-Todorova, Branimira, Dr. (0.50 Saxony-Anhalt, enhanced, demonstrating an important cross-talk between till 31.08.2010) histone marks and RdDM (DFG SFB 648, M. Kuhlmann, M.F. Bruchmüller, Astrid (0.50 Overhead, till 31.03.2010) Mette). Jovtchev, Gabriele, Dr. (0.50 Saxony-Anhalt, 04.05.-31.08.2010) Kuhlmann, Markus, Dr. (DFG / SFB 648) In a collaborative approach to harness minichromosomes as shuttles for recombinant gene expression in cereals, Visiting Scientists/Scholars chromosome truncation via transgene-induced telomer Bruchmüller, Astrid (self-financed, 01.04.-30.09.2010) seeding has been established. For this, T-DNA constructs Jachimoviciute, Simona (self-financed/IPK, 06.06.-18.06.2011) containing blocks of A. thaliana telomeric repeats were Plochotnikova, Alexandra (self-financed /I PK, 06.06.-18.06.2011) assembled and transferred into the genomes of A. thaliana and barley via Agrobacterium-mediated transformation. In both plant species, formation of transgene-associated telomeres Goals correlated with the truncation of chromosomes was readily observed. The truncated chromosomes were somatically stable Analysis and utilisation of epigenetic control mechanisms and seed-transmissible, although at a lower rate than to be acting at the chromatin level to warrant regulation and expected from Mendelian segregation (Pakt für Forschung structural maintenance of plant genomes. und Innovation, C.H. Teo, M. F. Mette, in collaboration with Karyotype Evolution, Chromosome Structure and Function and Plant Reproductive Biology, Dept. of Physiology and Cell Research Report Biology).

In plants, short interfering (si) RNAs can act as a nucleotide The way how chromosomes are arranged in interphase nuclei is sequence-specific signal for the induction of cytosine considered to affect DNA replication, transcription, and repair. methylation in genomic DNA. If this RNA-directed DNA Thus, the architecture of interphase nuclei in A. thaliana has methylation (RdDM) is targeted to homologous promotor been approached by fluorescencein situ hybridisation (FISH) or regions, it can result in RNA-directed transcriptional gene chromatin tagging using lacI-GFP fusion proteins and transgenic silencing (RdTGS). lacO tandem repeats. However, FISH can be performed only on fixed material and presence of transgenic lacO tandem repeats The genetic dissection of RdTGS in Arabidopsis thaliana by potentially alters the chromosome arrangement in interphase several groups has identified a considerable number of factors nuclei (G. Jovtchev, B.E. Borisova, M. Kuhlmann, J. Fuchs, K. required for this process. The majority of these proteins are Watanabe, I. Schubert and M.F. Mette 2011). As alternative, involved in siRNA generation and metabolism, DNA methylation transcription activator–like effector (TALE)-based engineered or chromatin remodeling. To identify the complete spectrum DNA binding proteins are being tested for their ability to target of factors essential for RdTGS, a screen for EMS-induced endogenous sequences in live plant nuclei. Initial results (see mutations showing a “suppressor of silencing” phenotype was Fig. 30, p. 74) indicate successful targeting of 45S rDNA by GFP- performed using a transgene system with particularly efficient fusion proteins specifically binding to a conserved sequence in transcriptional silencing. Mutant lines with a stable release of region coding for 5.8S rRNA. The approach will be extended to reporter gene expression and reduced cytosine methylation in further repetitive sequences (DFG proposal submitted, C.H. Teo, non-CG context indicative for a defect in RdDM were crossed to M. F. Mette, in collaboration with research group Chromosome accession L-er to initiate rough mapping of the affected genes. Structure and Function and J. Boch, MLU Halle-Wittenberg). Subsequent fine mapping and complementation tests have so far localised mutations in three genes known to be involved in RdDM, proving the functionality of the approach. The mapping of new mutations is ongoing (IPK funded, A. Finke, M. F. Mette).

73 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Fig. 30 Targeting of endogenous repetitive sequences by TALE-derived DNA-binding domain-GFP fusion proteins. A) Sketch of T-DNA cassette for protein expression. B - D) Microscopic GFP-fluorescence in vivo images of lower epidermal cells of Agrobacterium-infiltrated spots in Nicotiana benthamiana leaves. B) Agrobacterium without T-DNA construct. C) T-DNA construct without specificTA LE-derived DNA-binding domain. D) T-DNA construct with TALE-derived DNA-binding domain targeting the 5.8S rDNA of N. benthamiana. Arrows indicate GFP-labeled nucleoli. The scale bar indicates 10 µm (C.H. Teo).

Publications Jo v t c h e v , G., B.E. Bo r i s o v a , M. Ku h l m a n n , J. Fu c h s , K. Wa t a n a b e , I. Sc h u b e r t & M.F. Me t t e : Pairing of lacO tandem repeats in Arabidopsis Peer Reviewed Papers thaliana nuclei requires the presence of hypermethylated, large arrays at two chromosomal positions, but does not 2010 depend on H3-lysine-9-dimethylation. Chromosoma 120 Ba n a e i Mo g h a d d a m , A.M., J. Fu c h s , T. Cz a u d e r n a , A. Ho u b e n & M.F. (2011) 609-619. Me t t e : Intraspecific hybrids of Arabidopsis thaliana revealed Te o , C.H., L. Ma, E. Ka p u s i , G. He n s e l , J. Ku m l e h n , I. Sc h u b e r t , A. Ho u b e n no gross alterations in endopolyploidy, DNA methylation, & M.F. Me t t e : Induction of telomere-mediated chromosomal histone modifications and transcript levels. Theor. Appl. truncation and stability of truncated chromosomes in Genet. 120 (2010) 215-226. Arabidopsis thaliana. Plant J. 68 (2011) 28-39. Ka c z m a r c z y k , A., A. Ho u b e n , E.R.J. Ke l l e r & M.F. Me t t e : Influence of cryopreservation on the epigenetic state of potato. PhD and Diploma Theses CryoLetters 31 (2010) 380-391. 2010 Ba n a e i Mo g h a d d a m , A.M.: Dynamics of chromatin modifications 2011 and other nuclear features in response to intraspecific Ba n a e i Mo g h a d d a m , A.M., F. Ro u d i e r , M. Se i f e r t , C. Be r a r d , M.L. hybridization in Arabidopsis thaliana. (PhD Thesis) Martin- Ma g n i e t t e , R. Ka r i m i As h t i y a n i , A. Ho u b e n , V. Co l o t & M.F. Me t t e : Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Additive inheritance of histone modifications in Arabidopsis Fakultät I, Halle/S. (2010) 143 pp. thaliana intra-specific hybrids. Plant J. 67 (2011) 691-700.

74 Research Group: In vitro Differentiation (till 31 December 2010) Head: Prof. Anna M. Wobus

Scientists Dedifferentiation and reprogramming of cord blood (CB)- derived cells: Dedifferentiation and epigenetic modification Grant Positions (i.e. by TSA, 5-azaC) of CB-derived CD14+ cells showed only Sulzbacher, Sabine, Dr. (BMBF, till 31.12.2010) limited efficiency of reprogramming to pluripotency. Repeated (4 times) transfections of adult mouse and human cells with Visiting Scientists/Scholars viral vectors expressing the three pluripotency-associated Schroeder, Insa, Dr. (Martin Luther University Halle-Wittenberg, transcription factors (Oct4, Sox2, Klf4) were necessary to till 31.12.2010) partially reprogram CB-derived mesenchymal stem cells Daniel-Wojcik, Anna (self-financed, 01.03.-31.08.2010) (MSCs) into an ES-like state and to up-regulate transcript levels Sharif Panah, Elham, Dr. (self-financed, 22.02.-25.02.2010) of pluripotency markers (dissertation by A. Daniel-Wojcik; collaboration with A. Finkensieper, M. Wartenberg). The work has been continued by using further reprogramming strategies Goals at the University of Halle.

Research of the “In vitro Differentiation” group was dedicated Cardiac differentiation of ES cells and selective induction to the analysis of regulatory mechanisms during in vitro of pacemaker cells: Based on previous studies of the “In differentiation of mouse embryonic stem (ES) cells into the vitro-Differentiation” group (Wiese et al. 2011) showing the endoderm and pancreatic as well as cardiogenic lineages. In selective differentiation of sinus node-like cells by Suramin, the parallel, dedifferentiation and reprogramming strategies have collaboration with Dr. Alexander Kleger and Dr. Stefan Liebau been applied to human cord blood-derived cells to enhance at the University of Ulm resulted in the identification of a new their developmental potential. mechanism for the generation of cardiac and pacemaker-like cells by the activation of Ca2+-activated potassium channels (Kleger et al. 2010; Liebau et al., in press). Research Report Comparative analysis of human ES cell research in Germany The following results have been achieved during the reporting and worldwide: period (2010-2011): Working as guest scientist in the Department of Cytogenetics in 2011, studies of A.M. Wobus on human (h) ES cell research Endoderm differentiation, selection and characterisation resulted in reviews on the present state and future perspectives of Sox17-expressing ES cells: of pluripotent stem cells in toxicology (Wobus and Löser 2011) The pancreatic differentiation potential of ES cells was and on the performance of hES cell research in Germany in investigated by transcriptional profiling and cell biological international comparison (Löser et al. 2011; Löser et al. 2011; analysis. Specifically, Sox17-DsRed-puror expressing ES cells Wobus and Löser, in press; collaboration with Dr. P. Löser, RKI were differentiated with and without activin A induction. The Berlin). number of Sox17-DsRed-positive cells was measured by flow cytometry (FACS), which allowed the determination of the transcriptional profile and the developmental potential of Sox17+ cells. Differentiating ES cells revealed the capacity to develop into mesendoderm (Brachyury+)-, definitive endoderm (Cxcr4+, Foxa2+, Sox17+)-, primitive gut (Hnf4+)- and posterior foregut (Pdx1+)-positive cells. The pancreatic precursor cells had the potential to develop into the three pancreatic lineages of ductal (CK19+), exocrine (Hes1+) and endocrine (Nkx6.1+, Nkx2.2+, Ngn3+) cells. In contrast, activin A induction decreased the differentiation into ectoderm (Pax6+,N euroD6+) and primitive endoderm (Sdf1+, Sox7+, Afp+) cells (Schroeder et al., CTO in press; collaboration with I. Schroeder, MLU Halle, M. Solimena, R. Meisterfeld, TU Dresden). The experiments are ongoing at the University of Halle.

75 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Publications Books and Book Chapters

Peer Reviewed Papers 2010 Wo b u s , A.M., U. Wo b u s & B. Pa r t h i e r (Eds.): Der Begriff der Natur 2010 – Wandlungen unseres Naturverständnisses und seine Fol- Kl e g e r , A., T. Se u f f e r l e i n , D. Ma l a n , M. Ti s c h e n d o r f , A. St o r c h , A. gen. Gaterslebener Begegnung 2009. Nova Acta Leopoldi- Wo l h e i m , S. La t z , S. Pr o t z e , M. Po r z n e r , C. Pr o e p p e r , C. Br u n n e r , S.F. na, Bd. 109, Heft 376, Wissenschaftliche Verlagsgesellschaft, Ka t z , G. Va r m a Pu s a p a t i , L. Bu l l i n g e r , W.M. Fr a n z , R. Ko e h n t o p , K. Stuttgart (2010) 266 pp. Gi e h l , A. Sp y r a n t i s , O. Wittekindt, Q. Lin, M. Ze n k e , B.K. Fl e i s c h m a n n , Wo b u s , A.M. & U. Wo b u s : Gaterslebener Begegnung 2009. Der M. Wa r t e n b e r g , A.M. Wo b u s , T.M. Bo e c k e r s & S. Li e b a u : Modulation Begriff der Natur – Wandlungen unseres Naturverständ- of calcium-activated potassium channels induces nisses und seine Folgen. In: Jahrbuch 2009 der Deutschen cardiogenesis of pluripotent stem cells and enrichment of Akademie der Naturforscher Leopoldina Halle/S. LEOPOL- pacemaker-like cells. Circulation 122 (2010) 1823-1836. DINA (R. 3), 55 (2010) 419-420. Lö s e r , P., J. Sc h i r m , A. Gu h r , A.M. Wo b u s & A. Ku r t z : Human embryonic stem cell lines and their use in International Research. Stem Cells 28 (2010) 240-246. Other Papers Ro l l e t s c h e k , A., I.S. Sc h r o e d e r , H. Sc h u l z , O. Hu m m e l , N. Hu e b n e r & 2011 A.M. Wo b u s : Characterization of mouse embryonic stem Lö s e r , P., B. Ha n k e & A.M. Wo b u s : Humane pluripotente Stamm- cell differentiation into the pancreatic lineage in vitro zellen – Perspektiven ihrer Nutzung und die Forschungs- by transcriptional profiling, quantitative RT-PCR and situation in Deutschland. Naturwiss. Rundschau 64 (2011) immunocytochemistry. Int. J. Dev. Biol. 54 (2010) 41-54. 453-465. Se u f e r t , G., G. Pä t h , P. Fe i l e n , C. Kr a u t z , I. Sc h r ö d e r , A.M. Wo b u s , E. La m m e r t & M. So l i m e n a : Mechanismen und Methoden der Regeneration und des Ersatzes von Insulin produzierenden PhD and Diploma Theses Beta-Zellen des endokrinen Pankreas bei Diabetes mellitus. 2011 Med. Welt 61 (2010) 87-93. Da n i e l -Wo j c i k , A.: Differentiation, dedifferentiation and repro- Wo b u s , A.M.: The Janus face of pluripotent stem cells: Connection gramming experiments using embryonic stem and cord between pluripotency and tumourigenicity. Bioessays 32 blood-derived cells. (PhD Thesis) Martin-Luther-Universität (2010) 993-1002. Halle-Wittenberg, Naturwissenschaftliche Fakultät I, Institut für Biologie, Halle/S. (2011) 153 pp. 2011 Wi e s e , C., T. Ni k o l o v a , I. Za h a n i c h , S. Su l z b a c h e r , J. Fu c h s , S. Ya m a n a k a , E. Gr a f , U. Ra v e n s , K.R. Bo h e l e r & A.M. Wo b u s : Differentiation Additional Publication 2009 induction of mouse embryonic stem cells into sinus node- Be i e r , H.M., B. Fe h s e , B. Fr i e d r i c h , M. Gö t z , I. Ha n s m a n n , F. Hu c h o , K. like cells by suramin. Int. J. Cardiol. 147 (2011) 95-111. Kö c h y , B. Mü l l e r -Rö b e r , H.-J. Rh e i n b e r g e r , J. Re i c h , H.-H. Ro p e r s , Wo b u s , A.M. & P. Lö s e r : Present state and future perspectives of H.R. Sc h ö l e r , B. Sc h ö n e -Se i f e r t , K. Sp e r l i n g , K. Ta n n e r , J. Ta u p i t z & using pluripotent stem cells in toxicology research. Arch. A.M. Wo b u s (Eds.): Neue Wege in der Stammzellforschung - Toxicol. 85 (2011) 79-117. Reprogrammierung von differenzierten Körperzellen. Ber- lin-Brandenburgische Akademie der Wissenschaften, Berlin (2009) 28 pp.

76 Programme: Genome Analysis Research Group: Transcriptome Analysis Head: Dr. Patrick Schweizer Scientists Genes for nonhost resistance: The genes of interest were derived from a TIGS (Transient Induced Gene Silencing) IPK financed screening in barley epidermal cells attacked by the wheat Himmelbach, Axel, Dr. (Pakt für Forschung und Innovation, powdery mildew (Bgt). Out of 10 identified RNR (Required for 01.05.2010-31.10.2011) Nonhost Resistance) genes we examined three, RNR5 encoding Liu, Luo (0.50, 01.07.-31.12.2010) a partial duplicate of an U-box ARM-repeat E3 ligase, RNR6 encoding a cellulose synthase-like protein of the D clade Grant Positions (CSLD2) and RNR8 encoding an LRR-containing receptor-like Chen, Wanxin, Dr. (BMBF, till 31.03.2010; 0.50 Overhead, kinase (RLK) in greater detail. For RNR5 encoding the “sticky” 01.04.-30.04.2010; BMBF, since 01.05.2010) protein-protein interaction ARM domain of the ancient U-box E3 Douchkov, Dimitar, Dr. (BMBF, till 30.04.2011; 0.75 Overhead, ligase, we have identified 6 strongly interacting plant proteins 01.05.-30.06.2011; BMBF, since 01.07.2011) in a yeast 2-hybrid screening using a prey library from barley Hasan, Mainul (0.50 BMBF, since 01.11.2011) powdery mildew (Bgh)-attacked barley leaves, in collaboration Himmelbach, Axel, Dr. (0.75 BMBF, till 30.04.2010) with TU München. These 6 potential in vivo interactors are now Nowara, Daniela, Dr. (Industry, since 15.04.2010) analysed with respect to transcript regulation, TIGS phenotype, Rajaraman, Jeyaraman (0.50 DFG) and in vivo interaction with RNR5 by split YFP assay. Transgenic Stengel, Doreen (0.50 BMBF, till 30.04.2010) T1 populations of events exhibiting strong RNR5 silencing were identified and will be characterised for Bgh and Bgt resistance. Visiting Scientists/Scholars New transcripts with a clear differential regulation between Baum, Tobias (BMBF, 09.03.-30.06.2011) host- and nonhost responses in barley and wheat are being Ihlow, Alexander, Dr. (TH Ilmenau, till 30.11.2011) identified in a large-scale transcript profiling approach using Liu, Luo (self-financed, till 30.06.2010) 44K Agilent arrays of barley and wheat hybridised with RNA Marzin, Stephan (self-financed, till 31.01.2010) samples from inoculated, peeled epidermis in order to obtain Metzner, Ernst (self-financed, till 31.07.2010) a comprehensive picture of nonhost-related regulation events Pliego Prieto, Clara, Dr. (self-financed, 20.09.-20.10.2010) in Triticeae cereals (J. Rajaraman). For RNR6, evidence for an Siegwart, Gerald (self-financed, 20.09.-16.10.2010) important role in race-nonspecific host as well as nonhost resistance has been obtained using transgenic RNAi lines. The transgenic super-susceptible phenotype of these lines could be rescued by transient expression of a synthetic RNR6 gene, Goals which is immune to RNAi due to silent point mutations, a few hours prior to Bgt inoculation. Obviously, the HvCSLD2 gene is Gene regulation and -function in pathogen-attacked and involved in a dynamic local response to pathogen challenge drought-stressed cereals. representing a novel mechanism independent of lignification (see Fig. 31, p. 78). Although the enzymatic activity of the encoded CSL protein is still unknown, ongoing cell-wall analysis Research Report in collaboration with Adelaide University indicates a role in cellulose cristallinity and cell-wall thickness (D. Douchkov). For Phenomics and genes in pathogen-attacked barley: RNR8, the phenomenon of transgenomic complementation in According to current models, both nonhost- as well as race- wheat of nonhost-like resistance against Bgt could be confirmed nonspecific host resistance are manifestations of pathogen- using 55 additional receptor-like kinases of barley that are associated molecular pattern (PAMP)-triggered plant immunity associated with upregulated transcripts in Bgh-attacked leaves. and should be analysed in an integrative approach. Therefore, Approximately 13 additional RLKs were identified that induced the projects detailed functional analysis and application resistance in wheat to Bgt. These are now subcloned for further potential of barley genes for nonhost resistance and analyses (A. Himmelbach). identifying barley genes for race-nonspecific host resistance or -susceptibility are closely interconnected within the group, partly sharing the same candidate genes.

77 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Genes for race-nonspecific (basal) host resistance: In order to two proteins and optimise HIGS efficiency for plant protection identify genes that mediate race-nonspecific resistance of (see Fig. 25, p. 61). In the barley and wheat/FHB systems we are barley to Bgh we combined a functional-genomics approach characterising single and triple transgenic events targeting based on transcript profiling and automated TIGS (~1,400 either highly conserved fungal housekeeping genes or genes up-regulated by pathogen attack, belonging to defence- excellent virulence-related candidates. New functional HIGS related multigene families or mapping to a QTL confidence targets in FHB were identified in a transient-assay system of interval) with association-genetic (re-sequencing) and meta- adult wheat plants recently established in the lab that is based QTL mapping approaches. This guided us to a shortlist of on virus-induced gene silencing by the barley-stripe mosaic approximately 40 candidates with evidence for an important role virus (D. Nowara, W. Chen). in race-nonspecific resistance of barley. Candidates of special interest encode proteins involved in cell-death regulation such Phenomics of drought tolerance: as HvLsd1a or HvWRKY2 and in cell-wall re-shaping such as the The aim of this project is to identify new candidate genes for germin-like protein HvGER4 or cellulose-synthase like proteins osmotic stress (drought) tolerance in barley using a recently D2 and H1. Some of these candidates will be validated by stable developed, high-throughput transient-induced gene silencing transgenics as well as allele introgression by backcrossing assay (TIGS_drought). In this assay, stress tolerance is reflected (D. Douchkov, A. Johrde, A. Himmelbach). by the number of cells accumulating native dsRed reporter protein during a stress period of four days. In a collaboration Host-induced gene silencing (HIGS): with the lab of Luigi Cattivelli (CRA Fiorenzuola d’Arda, Italy) The novel phenomenon of HIGS in fungal pathogens was we addressed the role of a RING-type E3 ubiquitin ligase and further examined in barley and wheat attacked by powdery its in vitro as well as in vivo interaction partners in desiccation mildew and Fusarium head blight (FHB) fungi. In the barley/ tolerance of barley. As a result, TIGS of the E3 ligase enhanced Bgh HIGS system main emphasis lies on the identification of desiccation sensitivity of transformed leaf epidermal cells double-transgenic lines carrying RNAi constructs against both whereas transient over-expression rendered the cells more glucanosyltransferase GFT1 and GFT2 genes of the fungus. This robust against the stress, compared to the empty-vector should address the question of functional redundancy of the controls (S. Marzin).

Fig. 31 A high proportion of penetrated papillae of HvCSLD2-silenced barley plants are strongly autofluorescing. Examples of non-penetrated (n.p.) and penetrated (pen.) papillae of wildtype (wt) and transgenic plants (line E39), 48 h after inoculation with Blumeria graminis f. sp. tritici (Bgt). Arrows point at sites of attempted penetration (D. Douchkov). Bar = 25 µm.

78 Publications 2011 Ba u m , T., A. Na v a r r o -Qu e z a d a , W. Kn o g g e , D. Do u c h k o v , P. Sc h w e i z e r & Peer Reviewed Papers U. Se i f f e r t : HyphArea – Automated analysis of spatiotemporal fungal patterns. J. Plant Physiol. 168 (2011) 72-78. 2010 Do u c h k o v , D., A. Jo h r d e , D. No w a r a , A. Hi m m e l b a c h , S. Lu e c k , R. Ni k s & Ag h n o u m , R., T.C. Ma r c e l , A. Jo h r d e , N. Pe c c h i o n i , P. Sc h w e i z e r & R.E. P. Sc h w e i z e r : Convergent evidence for a role of WIR1 proteins Ni k s : Basal host resistance of barley to powdery mildew: during the interaction of barley with the powdery mildew connecting quantitative trait loci and candidate genes. Mol. fungus Blumeria graminis. J. Plant Physiol. 168 (2011) 20-29. Plant Microbe Interact. 23 (2010) 91-102. He n s e l , G., A. Hi m m e l b a c h , W. Ch e n , D.K. Do u c h k o v & J. Ku m l e h n : Ei c h m a n n , R., M. Bi s c h o f , C. We i s , J. Sh a w , C. La c o m m e , P. Sc h w e i z e r , Transgene expression systems in the Triticeae cereals. J. D. Do u c h k o v , G. He n s e l , J. Ku m l e h n & R. Hü c k e l h o v e n : BAX Plant Physiol. 168 (2011) 30-44. INHIBITOR-1 is required for full susceptibility of barley to Hü c k e l h o v e n , R. & P. Sc h w e i z e r : Quantitative disease resistance and powdery mildew. Mol. Plant Microbe Interact. 23 (2010) fungal pathogenicity in Triticeae. J. Plant Physiol. 168 (2011) 1217-1227. 1-2. Hi m m e l b a c h , A., L. Liu, U. Zi e r o l d , L. Al t s c h m i e d , H. Ma u c h e r , F. Be i e r , Sc h w e i z e r , P. & N. St e i n : Large-scale data integration reveals co- D. Mü l l e r, G. He n s e l , A. He i s e , A. Schützendübel , J. Ku m l e h n & P. localization of gene functional groups with meta-QTL for Sc h w e i z e r : Promoters of the barley germin-Like GER4 gene multiple disease resistance in barley. Mol. Plant Microbe cluster enable strong transgene expression in response to Interact. 24 (2011) 1492-1501. pathogen attack. Plant Cell 22 (2010) 937-952. Ko c s y , G., B. At h m e r , D. Pe r o v i c , A. Hi m m e l b a c h , A. Sz ü c s , I. Va s h e g y i , P. Sc h w e i z e r , G. Ga l i b a & N. St e i n : Regulation of gene expression Other Papers by chromosome 5A during cold hardening in wheat. Mol. 2010 Genet. Genomics 283 (2010) 351-363. Sc h w e i z e r , P., R. Hü c k e l h o v e n , W. Kn o g g e & U. Se i f f e r t : Mit No w a r a , D., A. Ga y , C. La c o m m e , J. Sh a w , C. Ri d o u t , D. Do u c h k o v , G. Phänomanalyse den Pilz-Pflanzen-Interaktionen auf der He n s e l , J. Ku m l e h n & P. Sc h w e i z e r : HIGS: host-induced gene Spur. GenomXPress 4 (2010) 7-9. silencing in the obligate biotrophic fungal pathogen Blumeria graminis. Plant Cell 22 (2010) 3130-3141. Se č e n j i , M., Á. Le n d v a i , P. Mi s k o l c z i , G. Ko c s y , Á. Ga l l é , A. Sz u c s , B. PhD and Diploma Theses Ho f f m a n n , É. Sá r v á r i , P. Sc h w e i z e r , N. St e i n , D. Du d i t s & J. Gy ö r g y e y : 2010 Differences in root functions during long-term drought Jo h r d e , A.: Assoziationsstudie zur Resistenz gegen den echten adaptation: comparison of active gene sets of two wheat Gerstenmehltau (Blumeria graminis f.sp. hordei) in einer ge- genotypes. Plant Biol. 12 (2010) 871-882. netisch diversen Gerstenpopulation (Hordeum vulgare L.). Ze l l e r h o f f , N., A. Hi m m e l b a c h , W.B. Do n g , S. Bi e r i , U. Sc h a f f r a t h & P. (PhD Thesis) Martin-Luther-Universität Halle-Wittenberg, Sc h w e i z e r : Nonhost resistance of barley to different fungal Naturwissenschaftliche Fakultät III, Halle/S. (2010) 165 pp. pathogens is associated with largely distinct, quantitative Ma r z i n , S.: Entwicklung einer RNAi basierten Screeningmethode transcriptional responses. Plant Physiol. 152 (2010) 2053- zur Charakterisierung der Trockenstressantwort bei Gerste 2066. (Hordeum vulgare L.). (PhD Thesis) Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I, Halle/S. (2010) 150 pp.

79 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Research Group: Gene and Genome Mapping Head: Dr. Marion Röder

Scientists Goals

IPK financed Exploitation of the natural genetic diversity in plants for Hanemann, Anja, Dr. (0.75 Pakt für Forschung und Innovation, identification, genetic mapping and cloning of genes for till 12.06.2010; 0.25/1.00, till 31.12.2010) agronomically important traits in cereals. Kollers, Sonja, Dr. (0.75, 01.07.-30.09.2011) Marzin, Stephan, Dr. (0.50/0.75 Pakt für Forschung und Innovation, 01.02.-31.12.2010; 0.25/1.00, 01.05.2010- Research Report 14.02.2011) Paliwal, Rajneesh (0.50 Pakt für Forschung und Innovation, A map-based cloning effort for the barley scald resistance 01.07.-31.12.2010) gene Rrs2 has resulted in a family of expressed pectin esterase Sharma, Shailendra, Dr. (Pakt für Forschung und Innovation, inhibitor genes (PEI1-PEI4) as possible candidates (S. Sharma). A till 31.03.2010) virus-induced gene silencing (VIGS)-based assay was developed Weichert, Nicola, Dr. (Pakt für Forschung und Innovation, and improved to achieve functional screening of candidate 01.01.-31.12.2010) genes in Rhynchosporium-infected barley plants. First results Worch, Sebastian, Dr. (Pakt für Forschung und Innovation, indicate that silencing of the PEI2 target gene in barley stripe 01.10.-31.12.2010) mosaic virus (BSMV)-mediated transient transformation assays increased susceptibility and hence facilitated fungal growth Grant Positions (see Fig. 32, p. 81) (S. Marzin). Kollers, Sonja, Dr. (BMBF, till 30.06.2011; 0.25 Overhead, Additionally, stably transformed plants were generated in col- 01.07.-30.09.2011) laboration with the research group Plant Reproductive Biology Marzin, Stephan, Dr. (DFG, since 15.02.2011) (J. Kumlehn, G. Hensel), in which the PEI2 gene is over-expressed Matthies, Inge, Dr. (BMBF, till 28.02.2011) in the background of the susceptible cultivar ‘Golden Promise’. Worch, Sebastian, Dr. (BMBF, till 30.09.2010) The fine mapping of a QTL for grain size on wheat chromo- Visiting Scientists/Scholars some 7D was continued in collaboration with J. Doležel and Absattarova, Aiman (IAOE Fellowship, 11.01.-19.03.2010) H. Simkova (Institute of Experimental Botany, Olomouc, Czech Castellanos, Thelma, Dr. (BMBF, 28.07.-29.09.2010) Republic). A number of 7D-specific wheat BACs from the QTL Diaz de Leon Alvarez, José Luis, Dr. (BMBF, 28.07.-29.09.2010 containing region were obtained and 454-sequenced in collab- and 04.09.-04.10.2011) oration with the research group Genome Diversity (N. Stein, A. Ilyas, Mehmoona (Research Fellowship of Higher Education Himmelbach). The BAC sequences were annotated and used for Commission, since 18.08.2011) further marker enrichment in the region of interest (N. Weichert, Kifetew Haile, Jemanesh (DAAD) M. Röder). Kumar, Uttam, Dr. (BMBF, since 01.06.2011) Leonova, Irina, Dr. (DFG, 10.10.-09.12.2010) The potential use of historic data was tested in a genome-wide Mora, Merit (BMBF, 03.08.-29.09.2010) association study of European barley cultivars using diversity Paliwal, Rajneesh (DAAD Fellowship, till 30.06.2010) arrays technology (DArT) markers. In collaboration with F. v. Salina, Elena, Dr. (BMELV, 04.10.-23.10.2010) Eeuwijk and M. Malosetti (Biometris, University Wageningen, The Netherlands) a model based on best linear unbiased estimations (BLUEs) was applied for association analysis of an unbalanced phenotypic dataset based on historic data for malting and kernel quality with varying varieties over years (I. Matthies).

80 Fig. 32 Rhynchosporium infection on barley seedlings and preliminary results of qRT-PCR analysis. Left: Pictures A-D show typical symptoms of Rhynchosporium infection on the susceptible cv. SteffiA ( , C) plants and the resistant cv. Atlas (B, D), 17 days after inoculation with isolate Sachs147-1. Right: DNA-abundance of Rhynchosporium-DNA in infected plants 13 days after inoculation determined by qRT-PCR. BSMV-mediated transient transformation was used for silencing of the candidate gene PEI2 and caused an increase of fungal DNA in comparison to the untreated control in both cultivars, indicating the importance of PEI2 as a scald resistance gene. (Sachs147-1 = Rhynchosporium isolate; BSMV = Barley stripe mosaic virus; MCS = empty multiple cloning site; PEI2 = Pectinesterase inhibitor gene 2) (S. Marzin).

A comprehensive set of phenotypic and genotypic data was Publications generated for 358 European winter wheat varieties. Genome- wide association analysis was performed for agronomic and Peer Reviewed Papers baking quality traits, as well as for resistance to the fungal pathogens Fusarium culmorum and Fusarium graminearum, 2010 Septoria tritici and Drechslera tritici-repentis in collaboration with Ac h t a r , S., M.Y. Mo u a l l a , A. Ka l h o u t , M.S. Rö d e r & N. Mi r a l i : B. Rodemann (Julius Kühn Institute, Braunschweig) (S. Kollers). Assessment of genetic diversity among Syrian durum (Triticum turgidum ssp. durum) and bread wheat (Triticum The impact of drought stress on the regulatory networks of aestivum L.) using SSR markers. Russ. J. Genet. 46 (2010) barley seed development was investigated in an introgression 1320-1326. line population in collaboration with N. Sreenivasulu (research Ca s t i l l o , A., H. Bu d a k , C. Ma r t í n , G. Do r a d o , A. Bö r n e r , M. Rö d e r & group Stress Genomics). Morphological and biochemical P. H e r n a n d e z : Interspecies and intergenus transferability of QTL for kernel traits were discovered and the expressions of barley and wheat D-genome microsatellite markers. Ann. candidate genes were monitored using a quantitative Real Appl. Biol. 156 (2010) 347-356. Time PCR approach (S. Worch). Ch e n , G.X., T. Kr u g m a n , T. Fa h i m a , K.G. Ch e n , Y.G. Hu, M. Rö d e r , E. Ne v o & A. Ko r o l : Chromosomal regions controlling seedling drought The mapping of quantitative resistance against the stem rust resistance in Israeli wild barley, Hordeum spontaneum C. pathovar UG99 was performed in a Triticum durum wheat Koch. Genet. Resour. Crop Evol. 57 (2010) 85-99. population and markers linked to stem rust resistance were De Le ó n , J.L.D., R. Escoppinichi , R. Za v a l a -Fo n s e c a , T. Ca s t e l l a n o s , applied on a set of T. durum cultivars and landraces (J.K. Haile) M.S. Rö d e r & A. Mu j e e b -Ka z i : Phenotypic and genotypic characterization of salt-tolerant wheat genotypes. Cereal Res. Commun. 38 (2010) 15-22. Ga r c i a -Su a r e z , J.V., J.L.D. De Le ó n & M.S. Rö d e r : Identification of QTLs and associated molecular markers related to starch degradation in wheat seedlings (Triticum aestivum L.) under saline stress. Cereal Res. Commun. 38 (2010) 163-174.

81 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Ga r c i a -Su a r e z , J.V., M.S. Rö d e r & J.L.D. De Le ó n : Identification of 2011 QTLs and associated molecular markers of agronomic traits in Di a z d e Le o n , J.L., R. Escoppinichi , N. Ge r a l d o , A. Bö r n e r & M.S. Rö d e r : wheat (Triticum aestivum L.) under two conditions of nitrogen The performance of single chromosome substitution lines fertilization. Cereal Res. Commun. 38 (2010) 459-470. of bread wheat subjected to salinity stress. Cereal Res. Ke r f a l , S., P. Gi r a l d o , M. Ro d r í g u e z -Qu i j a n o , J.F. Vá z q u e z , K. Ad a m s , Commun. 39 (2011) 317-324. O.M. Lu k o w , M.S. Rö d e r , D.J. So m e r s & J.M. Ca r r i l l o : Mapping Di a z d e Le o n , J.L., R. Escoppinichi , N. Ge r a l d o , T. Ca s t e l l a n o s , A. Mu j e e b - quantitative trait loci (QTLs) associated with dough quality Ka z i & M.S. Rö d e r : Quantitative trait loci associated with in a soft × hard bread wheat progeny. J. Cereal Sci. 52 (2010) salinity tolerance in field grown bread wheat.E uphytica 181 46-52. (2011) 371-383. Kh l e s t k i n a , E.K., U. Ku m a r & M.S. Rö d e r : Ent-kaurenoic acid oxidase Hu a n g , X.Q. & M.S. Rö d e r : High-density genetic and physical genes in wheat. Mol. Breed. 25 (2010) 251-258. bin mapping of wheat chromosome 1D reveals that Kh l e s t k i n a , E.K., M.S. Rö d e r & A. Bö r n e r : Mapping genes controlling powdery mildew resistence gene Pm24 is located in a highly anthocyanin pigmentation on the glume and pericarp in recombinogenic region. Genetica 139 (2011) 1179-1187. tetraploid wheat (Triticum durum L.). Euphytica 171 (2010) Kh l e s t k i n a , E.K., E.A. Sa l i n a , I.E. Ma t t h i e s , I.N. Le o n o v a , A. Bö r n e r & 65-69. M.S. Rö d e r : Comparative molecular marker-based genetic Kh l e s t k i n a , E.K., M.S. Rö d e r , T.A. Pshenichnikova & A. B ö r n e r : Functional mapping of flavanone 3-hydroxylase genes in wheat, rye diversity at the Rc (red coleoptile) gene in bread wheat. Mol. and barley. Euphytica 179 (2011) 333-341. Breed. 25 (2010) 125-132. Le o n o v a , I.N., E.B. Bu d a s h k i n a , N.P. Ka l i n i n a , M.S. Rö d e r , A. Bö r n e r Ku m a r , U., A.K. Jo s h i , S. Ku m a r , R. Ch a n d & M.S. Rö d e r : Quantitative & E.A. Sa l i n a : Triticum aestivum × Triticum timopheevii trait loci for resistance to spot blotch caused by Bipolaris introgression lines as a source of pathogen resistance genes. sorokiniana in wheat (T. aestivum L.) lines ‘Ning 8201’ and Czech J. Genet. Plant Breed. 47 (2011) S49-S55. ‘Chirya 3’. Mol. Breed. 26 (2010) 477-491. Wi t z e l , K., C. Pi e t s c h , M. St r i c k e r t , A. Ma t r o s , M. Rö d e r , W. We s c h k e , Ku m a r , U., A.K. Jo s h i , M. Ku m a r i , R. Pa l i w a l , S. Ku m a r & M.S. Rö d e r : U. Wo b u s & H.P. Mo c k : Mapping of quantitative trait loci Identification of TQ Ls for stay green trait in wheat (Triticum associated with protein expression variation in barley grains. aestivum L.) in the ‘Chirya 3’ × Sonalika’ population. Euphytica Mol. Breed. 27 (2011) 301-314. 174 (2010) 437-445. Wo r c h , S., K. Ra j e s h , V.T. Harshavardhan , C. Pi e t s c h , V. Ko r z u n , L. Ku n t z e , Le o n o v a , L.N., E.B. Bu d a s h k i n a , K. Fl a t h , A. We i d n e r , A. Bö r n e r & M. A. Bö r n e r , U. Wo b u s , M.S. Rö d e r & N. Sreenivasulu : Haplotyping, Rö d e r : Microsatellite mapping of a leaf rust resistance gene linkage mapping and expression analysis of barley genes transferred to common wheat from Triticum timopheevii. regulated by terminal drought stress influencing seed Cereal Res. Commun. 38 (2010) 211-219. quality. BMC Plant Biol. 11 (2011) 1. Na v a k o d e , S., A. We i d n e r , R.K. Va r s h n e y , U. Lo h w a s s e r , U. Sc h o l z , M.S. Rö d e r & A. Bö r n e r : A genetic analysis of aluminium tolerance in cereals. Agriculturae Conspectus Scientificus 75 (2010) 191-196. Sa l e m , K.F.M., R.K. Va r s h n e y , M.S. Rö d e r & A. Bö r n e r : EST-SSR based estimates on functional genetic variation in a barley (Hordeum vulgare L.) collection from Egypt. Genet. Resour. Crop Evol. 57 (2010) 515-521. Sh a r m a , S., N. Sreenivasulu , V.T. Harshavardhan , C. Se i l e r , S. Sh a r m a , Z.N. Kh a l i l , E. Ak h u n o v , S.K. Se h g a l & M.S. Rö d e r : Delineating the structural, functional and evolutionary relationships of sucrose phosphate synthase gene family II in wheat and related grasses. BMC Plant Biol. 10 (2010) 134. Ti k h e n k o , N., N. Ts v e t k o v a , A. Vo y l o k o v , O. Dobrovolskaya , K. Za y n a l i Ne z h a d , M.S. Rö d e r & A. Bö r n e r : Embryo lethality in wheat 3 rye hybrids-mode of inheritance and the identification of a complementary gene in wheat. Euphytica 176 (2010) 191-198.

82 Books and Book Chapters Electronic Publications 2010 2011 Ma t t h i e s , I.E., S. We i s e , K. Fö r s t e r & M.S. Rö d e r : Association Bö r n e r , A., E.K. Kh l e s t k i n a , S. Ch e b o t a r , M. Na g e l , M.A. Re h m a n -Ar i f , mapping and marker development of the candidate K. Ne u m a n n , B. Ko b i l j s k i , U. Lo h w a s s e r & M.S. Rö d e r : Mainte- genes (1->3),(1->4)-β-D-Glucan-4-glucanohydrolase and nance and exploitation of genetic resources for future plant (1->4)-β-Xylan-endohydrolase 1 for malting quality in breeding. In: Ve i s z , O. (Ed.): Agrisafe Final Conference “Cli- barley. http://pgrc.ipk-gatersleben.de/glb, http://pgrc. mate change: Challenges and opportunities in agriculture” ipk-gatersleben.de/X-1 & http://pgrc.ipk-gatersleben.de/ (21.-23.03. 2011, Budapest/Hungary). Agricultural Research Phendat (2010). Institute of the Hungarian Academy of Sciences (2011) Ma t t h i e s , I.E., S. We i s e & M.S. Rö d e r : Association of haplotype 26-31. diversity in the α-amylase gene amy1 with malting quality parameters in barley. http://pgrc.ipk-gatersleben.de/alpha- amylase (2010). Other Papers We i s e , S., U. Sc h o l z , M.S. Rö d e r & I.E. Ma t t h i e s : MetaBrew: A 2010 comprehensive database of malting quality traits in brewing Bö r n e r , A., A.K. Jo s h i , E.K. Kh l e s t k i n a , B. Ko b i l j s k i , I. Kr a n n e r , U. Ku m a r , barley. http://metabrew.ipk-gatersleben.de (2010). S. La n d j e v a , L.N. Le o n o v a , U. Lo h w a s s e r , M. Na g e l , S. Navakode, K. Ne u m a n n , R. Pa l i w a l , M.A. Re h m a n Ar i f , M.S. Rö d e r , N. Ti k h e n k o , A. 2011 We i d n e r & K. Za y n a l i Ne z h a d : Items from Germany. Ann. Wheat Ma t t h i e s , I.E., S. We i s e , K. Fö r s t e r & M.S. Rö d e r : Association Newsl. 56 (2010) 47-52. mapping and marker development of the candidate Ha n e m a n n , A., G.F. Sc h w e i z e r & M.S. Rö d e r : Entwicklung und Vali- genes (1->3),(1->4)-β-D-Glucan-4-glucanohydrolase and dierung von diagnostischen Markern für das Rrs2-Resistenz- (1->4)-β-Xylan-endohydrolase 1 for malting quality in gen gegen Rhynchosporium secalis in Gerste. Tagungsband barley. http://pgrc.ipk-gatersleben.de/glb, http://pgrc. der 60. Jahrestagung der Vereinigung der Pflanzenzüchter ipk-gatersleben.de/X-1 & http://pgrc.ipk-gatersleben.de/ und Saatgutkaufleute Österreichs, 24.-26.11.2009, HBLFA Phendat (2011). Raumberg-Gumpenstein/Austria, (2010) 151-154. Ma t t h i e s , I.E., S. We i s e & M.S. Rö d e r : Association of haplotype Ma t t h i e s , I.E., S. We i s e & M.S. Rö d e r : Assoziationskartierung in Gers- diversity in the α-amylase gene amy1 with malting quality te – Ein genomweiter und Kandidatengenansatz. Tagungs- parameters in barley. http://pgrc.ipk-gatersleben.de/alpha- band der 60. Jahrestagung der Vereinigung der Pflanzen- amylase (2011). züchter und Saatgutkaufleute Österreichs, 24.-26.11.2009, We i s e , S., U. Sc h o l z , M.S. Rö d e r & I.E. Ma t t h i e s : MetaBrew: A HBLFA Raumberg-Gumpenstein/Austria (2010) 91-96. comprehensive database of malting quality traits in brewing Sreenivasulu , N., M.S. Rö d e r & U. Wo b u s : Trockenstress – eine Suche barley. http://metabrew.ipk-gatersleben.de (2011). nach den Ursachen und nach neuen Wegen zur Züchtung trockentoleranter Getreide. GenomXPress 4 (2010) 4-6. Additional Publications 2009 2011 Hovhannisyan , N., M.E. Du l l o o , A. Ye s a y a n , M.S. Rö d e r , H. Kn ü p f f e r , Bö r n e r , A., E.V. An t o n o v a , J.K. Ha i l e , E.K. Kh l e s t k i n a , B. Ko b i l j s k i , S. A. Am r i & A.M. Da n i e l i a n : Wild relatives of wheat as sources of Ko l l e r s , U. Lo h w a s s e r , M. Na g e l , K. Ne u m a n n , M.A. Re h m a n -Ar i f , N. useful genes. In: In Vitro Cellular & Developmental Biology- Ti k h e n k o , K. Za y n a l i Ne z h a d & M.S. Rö d e r : Items from Germany. Animal, Vol. 45, Springer, New York (2009) S82-S83. Ann. Wheat Newsl. 57 (2011) 12-15.

83 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Research Group: Bioinformatics and Information Technology Head: Dr. Uwe Scholz

Scientists Research Report

IPK financed The rapid progress in high-throughput experiments is directly Lange, Matthias, Dr. coupled with a strong growth of primary research data: To Steuernagel, Burkhard (Pakt für Forschung und Innovation, overcome the disadvantages of local storage of such data since 01.01.2011) within the research groups (e.g. missing long-term data access, problems in reusability and no citation possibilities fulfilling the Grant Positions German Research Council guidelines “Rules of good scientific Mascher, Martin (0.50/1.00 BMBF, since 01.02.2011) practice”) the IPK is now a data centre in the international Schmutzer, Thomas (0.50/1.00 BMBF, since 01.01.2010) DataCite consortium (http://www.datacite.org). In the frame of Steuernagel, Burkhard (BMBF, till 31.12.2010) this membership, an infrastructure for registering DOI citations Weise, Stephan, Dr. (BMBF, till 31.12.2010; Industry, was developed. Furthermore, systems like LIMS-Light and since 01.01.2011) eDAL are supporting the publication pipeline for primary research data (M. Lange, C. Colmsee, F. Flemming, B. Röhl, D. Visiting Scientists/Scholars Arend, J. Benz). Czernicka, Malgorzata, Dr. (DAAD, 28.02.2010-31.12.2011) Munoz Amatriain, Maria (University of Minnesota, With the LAILAPS search engine (http://lailaps.ipk-gatersle- 06.06.-17.07.2011) ben.de) we built a system with the aim to find relevant data in Schlüter, Urte, Dr. (self-financed, 06.06.-10.06.2011) non-integrated life science databases. The core of LAILAPS is a probabilistic model for relevance prediction based on neural networks. To consider the fact that data relevance is highly Goals subjective to the user of an information retrieval system, we support specific neural networks. The system is available for Development of information systems, data integration pipe- IPK databases as well as for external databases such as UniProt lines, life science search engines, and usage/adaptation of or the DAWIS data warehouse developed at Bielefeld Univer- bioinformatics tools for sequence analysis/assembly. Moreover, sity. Currently, an EU-scaled application is implemented in the the research group is responsible for the IT infrastructure of the frame of the EU-FP7 project “transPLANT” (M. Lange, J. Chen). whole institute as well as for the establishment and the operation of a laboratory information management system (LIMS). Within the BARLEX project we provided the data storage and assembly of 454 sequenced barcoded BAC pools. In total, assemblies for 3,500 BACs have been calculated. Additionally pipelines for whole-genome shotgun sequencing using Illumina platform were developed and implemented. Using these pipelines, those large datasets can now be de novo assembled, or mined for SNPs (B. Steuernagel and collaboration with the research group Genome Diversity).

84 In the frame of the “Pakt für Forschung und Innovation” project In close collaboration with the research groups Plant Bioin- “Sequencing of the Barley Chromosome 3H” the pipeline formatics and Systems Biology we continued to improve the for assembling BACs has been applied for further sequencing. MetaCrop information system managing manually curated More than 2,000 BACs have been assembled. New strategies data about crop plant metabolism. The web-based user inter- for sequencing pooled paired-end libraries of BACs have face was reworked and the SBML exporter has been enhanced. been evaluated and pilot-experiments have been analysed Furthermore, MetaCrop data is now indexed by the life science (B. Steuernagel and collaboration with the research groups search engine LAILAPS (S. Weise, M. Lange, J. Chen). Genome Diversity and Plant Bioinformatics). In cooperation with the Satellite Collections North of the IPK In the RYE-EXPRESS project we assembled over 2.5 million Genebank we are reengineering the Diversity Studies Toolkit RNA-seq reads into contigs representing a comprehensive (DiSTo) software. DiSTo2 provides a convenient Java-Swing- genomics resource for rye. From sequence comparisons based user interface for studying the genetic diversity of 5,234 single nucleotide polymorphisms (SNPs) were identified Lolium accessions, but is also applicable to other crop species. to develop the Rye5K high-throughput SNP genotyping array. The software enables researchers to perform comparative as The process has been integrated into a pipeline for detection well as multivariate analyses using phenotypic, genotypic and of single nucleotide polymorphism (T. Schmutzer and passport data (S. Weise). collaboration with the research group Genome Diversity). The BMBF-funded BioEnergy 2021 project OPTIMAS (since Within the CORNFED project we designed a yield related 2009) explores diverse biological processes with the aim NimbleGen Sequence Capture array. Over 4.5 k genes have been of increasing yield and biomass production in maize. We selected so far that led to first experimental studies with 454 established a database as an extension of the CropHouse reads from captured B73 lines. A new strategy and its prototype system and developed an Oracle Application Express (APEX)- pipeline has been developed for the workflow to study the based web interface for data retrieval and presentation. In 2010 diversity in maize lines (T. Schmutzer and collaboration with we extended our database to allow the storage of data from the research group Heterosis). different data domains. In 2011 we developed an APEX-based web platform, OPTIMAS-EDP, which allows to browse and In the GENOBAR project we were in charge of the management filter all experimental data and additionally to download the of primary data. Moreover, we developed the GENOBAR- data for further analysis. Following a correlation network-based WAREHOUSE, providing information for genome-wide approach we developed a high-throughput analysis pipeline association studies in barley based on phenotypic data as that integrates transcriptome, metabolite and phenotypic well as molecular markers (SNP, SSR, DArT and Illumina). We data to identify key metabolites and candidate genes for continued our work in the frame of this project (until 08/2010) yield and biomass production. In cooperation with the Plant and finished the development of the data warehouse (S. Weise Bioinformatics group, we established methods for comfortably and collaboration with the research groups Genome Diversity, visualising network data, exchanging analysis results as well Gene and Genome Mapping). as incorporating feedback from our experimentalist partners which will facilitate the validation of in silico predicted regulators (C. Colmsee, M. Mascher).

In addition, the research group works on the establishment of an IPK-wide laboratory information management system (LIMS). The results of a study performed by the research group led to the decision to establish the commercial system LIMSOPHY. Currently, use cases are implemented for several research groups (M. Lange, D. Schüler).

85 Abteilung Cytogenetik und Genomanalyse/Department of Cytogenetics and Genome Analysis

Publications Pi c k , T.R., A. Br ä u t i g a m , U. Sc h l u t e r , A.K. De n t o n , C. Co l m s e e , U. Sc h o l z , H. Fa h n e n s t i c h , R. Pi e r u s c h k a , U. Ra s c h e r , U. So n n e w a l d & A.P. We- Peer Reviewed Papers b e r : Systems analysis of a maize leaf developmental gradient redefines the current C4 model and provides candidates for 2010 regulation. Plant Cell 23 (2011) 4208-4220. Br e u i l l i n , F., J. Sc h r a m m , M. Ha j i r e z a e i , A. Ah k a m i , P. Fa v r e , U. Dr u e g e , Se i l e r , C., V.T. Harshavardhan , K. Ra j e s h , P.S. Re d d y , M. St r i c k e r t , H. B. Ha u s e , M. Bu c h e r , T. Kr e t z s c h m a r , E. Bo s s o l i n i , C. Ku h l e m e i e r , Ro l l e t s c h e k , U. Sc h o l z , U. Wo b u s & N. Sreenivasulu : ABA biosyn- E. Mar tinoia, P. Fr a n k e n , U. Sc h o l z & D. Re i n h a r d t : Phosphate thesis and degradation contributing to ABA homeostasis systemically inhibits development of arbuscular mycorrhiza during barley seed development under control and terminal in Petunia hybrida and represses genes involved in drought-stress conditions. J. Exp. Bot. 62 (2011) 2615-263. mycorrhizal functioning. Plant J. 64 (2010) 1002-1017. Ta u d i e n , S., B. St e u e r n a g e l , R. Ar i y a d a s a , D. Sc h u l t e , T. Sc h m u t z e r , Co c k r a m , J., J. Wh i t e , D.L. Zu l u a g a , D. Sm i t h , J. Co m a d r a n , M. M. Gr o t h , M. Fe l d e r , A. Pe t z o l d , U. Sc h o l z , K.F. Ma y e r , N. St e i n Ma c a u l a y , Z. Lu o , M.J. Ke a r s e y , P. We r n e r , D. Ha r r a p , C. Ta p s e l l , H. & M. Pl a t z e r : Sequencing of BAC pools by different next Liu, P.E. He d l e y , N. St e i n , D. Sc h u l t e , B. St e u e r n a g e l , D.F. Ma r s h a l l , generation sequencing platforms and strategies. BMC Res. W.T. Th o m a s , L. Ra m s a y , I. Ma c k a y , D.J. Ba l d i n g , T.A. Co n s o r t i u m , R. Notes 4 (2011) 411. Wa u g h & D.M. O’Su l l i v a n : Genome-wide association mapping We i s e , S., J. Ch e n , B.H. Ju n k e r , M. Klapperstück , M. La n g e , F. Sc h r e i b e r & to candidate polymorphism resolution in the unsequenced U. Sc h o l z : Das Informationssystem MetaCrop zur Unterstüt- barley genome. Proc. Natl. Acad. Sci. U.S.A. 107 (2010) 21611- zung systembiologischer Forschung an Kulturpflanzen. it - 21616. Information Technology 53 (2011) 234-240. Hi p p e , K., C. Co l m s e e , T. Cz a u d e r n a , E. Gr a f a h r e n d -Be l a u , B.H. Ju n k e r , Wi c k e r , T., K.F.X. Ma y e r , H. Gu n d l a c h , M. Ma r t i s , B. St e u e r n a g e l , U. C. Kl u k a s , U. S c h o l z , F. S c h r e i b e r & S. We i s e : Novel developments Sc h o l z , H. Ši m k o v á , M. Ku b a l á k o v á , F. Ch o u l e t , S. Ta u d i e n , M. of the MetaCrop information system for facilitating systems Pl a t z e r , C. Fe u i l l e t , T. Fa h i m a , H. Bu d a k , J. Do l e ž e l , B. Ke l l e r & N. biological approaches. J. Integr. Bioinform. 7 (2010) e125. St e i n : Frequent gene movement and pseudogene evolution La n g e , M., K. Sp i e s , J. Ba r g s t e n , G. Ha b e r h a u e r , M. Klapperstück , M. is common to the large and complex genomes of wheat, Le p s , C. We i n e l , R. Wu n s c h i e r s , M. We i s s b a c h , J. St e i n & U. Sc h o l z : barley, and their relatives. Plant Cell 23 (2011) 1706-1718. The LAILAPS search engine: relevance ranking in life science databases. J. Integr. Bioinform. 7 (2010) 110. La n g e , M., K. Sp i e s , C. Co l m s e e , S. Fl e m m i n g , M. Klapperstück & U. Sc h o l z : Books and Book Chapters The LAILAPS search engine: a feature model for relevance ranking in life science databases. J. Integr. Bioinform. 7 2010 (2010) 118. La n g e , M., S. We i s e , U. Sc h o l z , P. Ve r r i e r & C. Ra w l i n g s (Eds.): Proc. Na v a k o d e , S., A. We i d n e r , R.K. Va r s h n e y , U. Lo h w a s s e r , U. Sc h o l z , M.S. 6th Int. Symp. Integ. Bioinformatics 2010, 22.-24.03.2010, Rö d e r & A. Bö r n e r : A genetic analysis of aluminium tolerance Cambridge, UK (2010) 406 pp. in cereals. Agriculturae Conspectus Scientificus 75 (2010) 191-196. 2011 Ba c h m a n n , A., R. Sc h u l t , M. La n g e & M. Spiliopoulou : Extracting 2011 cross references from life science databases for search result Co l m s e e , C., S. Fl e m m i n g , M. Klapperstück , M. La n g e & U. Sc h o l z : A ranking. In: Be r e n d t , B., A. d e Vr i e s , W. Fa n , C. Ma c d o n a l d , I. Ou n i s case study for efficient management of high throughput & I. Ru t h v e n (Eds.): Proc. 20th ACM Conference on Information primary lab data. BMC Res. Notes 4 (2011) 413. and Knowledge Management (Glasgow, Scotland/UK, 24.- Ha s e n e y e r , G., T. Sc h m u t z e r , M. Se i d e l , R. Zh o u , M. Ma s c h e r , C.C. Sc h o n , 28.10.2011). ACM New York, NY, USA (2011) 1253-1258. S. Ta u d i e n , U. Sc h o l z , N. St e i n , K.F. Ma y e r & E. Ba u e r : From RNA- seq to large-scale genotyping – genomics resources for rye (Secale cereale L.). BMC Plant Biol. 11 (2011) 131. Other Papers Ma y e r , K.F.X., M. Ma r t i s , P.E. He d l e y , H. Ši m k o v á , H. Liu, J.A. Mo r r i s , B. 2010 St e u e r n a g e l , S. Ta u d i e n , S. Ro e s s n e r , H. Gu n d l a c h , M. Ku b a l á k o v á , Ma t t h i e s , I.E., S. We i s e & M.S. Rö d e r : Assoziationskartierung in P. Su c h á n k o v á , F. Mu r a t , M. Fe l d e r , T. Nu s s b a u m e r , A. Gr a n e r , J. Gerste – Ein genomweiter und Kandidatengenansatz. Sa l s e , T. En d o , H. Sa k a i , T. Ta n a k a , T. It o h , K. Sa t o , M. Pl a t z e r , T. Tagungsband der 60. Jahrestagung der Vereinigung der Ma t s u m o t o , U. Sc h o l z , J. Do l e ž e l , R. Wa u g h & N. St e i n : Unlocking Pflanzenzüchter und Saatgutkaufleute Österreichs, 24.- the barley genome by chromosomal and comparative 26.11.2009, HBLFA Raumberg-Gumpenstein/Austria (2010) genomics. Plant Cell 23 (2011) 1249-1263. 91-96.

86 Electronic Publications 2011 2010 Co l m s e e , C., C. Za n k e , T. Fu n k e , S. We i s e , E.R.J. Ke l l e r & U. Sc h o l z : The Co l m s e e , C., C. Za n k e , T. Fu n k e , S. We i s e , E.R.J. Ke l l e r & U. Sc h o l z : The Garlic and Shallot Core Collection (GSCC). http://www.ipk- Garlic and Shallot Core Collection (GSCC). http://www.ipk- gatersleben.de/databases/genetic_resources/gscc (2011). gatersleben.de/databases/genetic_resources/gscc (2010). Ke l l e r , E.R.J., C. Za n k e & U. Sc h o l z : EURALLIVEG – Vegetative Gr a f a h r e n d -Be l a u , E., S. We i s e , D. Ko s c h ü t z k i , U. Sc h o l z , B.H. Ju n k e r Allium, Europe’s Core Collection, Safe and Sound. http:// & F. Sc h r e i b e r : MetaCrop – A detailed database of crop plant euralliveg.ipk-gatersleben.de (2011). metabolism. http://metacrop.ipk-gatersleben.de (2010). La n g e , M., J. Ch e n & U. Sc h o l z : LAILAPS – The Life Science Search Ke l l e r , E.R.J., C. Za n k e & U. Sc h o l z : EURALLIVEG – Vegetative Engine. http://lailaps.ipk-gatersleben.de (2011). Allium, Europe’s Core Collection, Safe and Sound. http:// Ma t t h i e s , I.E., S. We i s e , K. Fö r s t e r & M.S. Rö d e r : Association euralliveg.ipk-gatersleben.de (2010). mapping and marker development of the candidate La n g e , M., J. Ba r g s t e n , M. We i s s b a c h , K. Sp i e s & U. Sc h o l z : LAILAPS genes (1->3),(1->4)-β-D-Glucan-4-glucanohydrolase and – The Life Science Search Engine. http://lailaps.ipk- (1->4)-β-Xylan-endohydrolase 1 for malting quality in gatersleben.de (2010). barley. http://pgrc.ipk-gatersleben.de/glb, http://pgrc. La n g e , M., T. Ru t k o w s k i , A. St e p h a n i k , B. St e u e r n a g e l & U. Sc h o l z : ipk-gatersleben.de/X-1 & http://pgrc.ipk-gatersleben.de/ FLAREX – The IPK array experiment database. http://pgrc. Phendat (2011). ipk-gatersleben.de/flarex (2010). Ma t t h i e s , I.E., S. We i s e & M.S. Rö d e r : Association of haplotype Ma t t h i e s , I.E., S. We i s e , K. Fö r s t e r & M.S. Rö d e r : Association diversity in the α-amylase gene amy1 with malting quality mapping and marker development of the candidate parameters in barley. http://pgrc.ipk-gatersleben.de/alpha- genes (1->3),(1->4)-β-D-Glucan-4-glucanohydrolase and amylase (2011). (1->4)-β-Xylan-endohydrolase 1 for malting quality in Sc h o l z , U., C. Kü n n e , M. La n g e , H. Mi e h e & T. Fu n k e : CR-EST: a resource barley. http://pgrc.ipk-gatersleben.de/glb, http://pgrc. for crop ESTs. http://pgrc.ipk-gatersleben.de/cr-est (2011). ipk-gatersleben.de/X-1 & http://pgrc.ipk-gatersleben.de/ Sc h r e i b e r , F., C. Co l m s e e , T. Cz a u d e r n a , E. Gr a f a h r e n d -Be l a u , A. Phendat (2010). Ha r t m a n n , A. Ju n k e r , B.H. Ju n k e r , D. Ko s c h ü t z k i , U. Sc h o l z & S. Ma t t h i e s , I.E., S. We i s e & M.S. Rö d e r : Association of haplotype We i s e : MetaCrop 2.0. http://metacrop.ipk-gatersleben.de diversity in the α-amylase gene amy1 with malting quality (2011). parameters in barley. http://pgrc.ipk-gatersleben.de/alpha- St e u e r n a g e l , B. & U. Sc h o l z : POCI annotation tool. http://pgrc.ipk- amylase (2010). gatersleben.de/poci (2011). Sc h o l z , U., C. Kü n n e , M. La n g e , H. Mi e h e & T. Fu n k e : CR-EST: a resource We i s e , S., I. Gr o s s e , C. Kl u k a s , D. Ko s c h ü t z k i , U. Sc h o l z , F. Sc h r e i b e r for crop ESTs. http://pgrc.ipk-gatersleben.de/cr-est (2010). & B.H. Ju n k e r : Meta-All: A system for managing metabolic St e u e r n a g e l , B. & U. Sc h o l z : POCI annotation tool. http://pgrc.ipk- pathway information. http://meta-all.ipk-gatersleben.de gatersleben.de/poci (2010). (2011). We i s e , S., N. Bi e r m a n n , S. Fl e m m i n g , J. Vo r w a l d , T. v a n Hi n t u m , H. We i s e , S., S. Ha r r e r , I. Gr o s s e , H. Kn ü p f f e r & E. Wi l l n e r : The European Kn ü p f f e r & I. Gr o s s e : Proposal for improvement of PGR data Poa Database (EPDB). http://poa.ipk-gatersleben.de (2011). exchange through XML. In: Proc. EPGRIS Final Conference, 11- We i s e , S., U. Sc h o l z , M.S. Rö d e r & I.E. Ma t t h i e s : MetaBrew: A 13 September 2003, Prague, Czech Republic. International comprehensive database of malting quality traits in brewing Plant Genetic Resources Institute (IPGRI). http://www.ecpgr. barley. http://metabrew.ipk-gatersleben.de (2011). cgiar.org/Networks/Info_doc/FinalMeeting Presentations/ Papers/Bierman_IPK_v0.14.doc (2010). We i s e , S., I. Gr o s s e , C. Kl u k a s , D. Ko s c h ü t z k i , U. Sc h o l z , F. Sc h r e i b e r & B.H. PhD and Diploma Theses Ju n k e r : Meta-All: A system for managing metabolic pathway 2011 information. http://meta-all.ipk-gatersleben.de (2010). Ba c h m a n n , A.: Anfrageranking in Suchmaschinen der Lebenswis- We i s e , S., S. Ha r r e r , I. Gr o s s e , H. Kn ü p f f e r & E. Wi l l n e r : The European senschaften unter Berücksichtigung der Bewertung von Poa Database (EPDB). http://poa.ipk-gatersleben.de (2010). Querverweisen. (Bachelor) Otto-von-Guericke Universität We i s e , S., U. Sc h o l z , M.S. Rö d e r & I.E. Ma t t h i e s : MetaBrew: A Magdeburg, Fakultät für Informatik, Magdeburg (2011) 71 pp. comprehensive database of malting quality traits in brewing Fr i e d r i c h , C.: Entwurf und Implementierung einer JAVA-API zum barley. http://metabrew.ipk-gatersleben.de (2010). Zugriff und zur DBMS-unabhängigen Persistierung von Bild- und Metadaten aus einer Pflanzenphänotypisierungs- anlage. (Diploma Thesis) Hochschule Harz, Fachbereich Au- tomatisierung und Informatik, Wernigerode (2011) 147 pp. Sc h ö n h e r r , I.S.: Assemblierung und Analyse des Vitis vinifera- Genoms der Rebsorte Riesling. (Bachelor) Martin-Luther- Universität Halle-Wittenberg, Institut für Informatik, Halle/S. (2011) 40 pp.

87 Wahlweise: Kapitel, Thema, Unterthema, Autor, Zusatzinformationen Abteilung Molekulare Genetik/ Department of Molecular Genetics

Abb. 33 Fig. 33 Erfassung sichtbarer Merkmale in automatisierten Phänotypisierungseinrich- Monitoring visible plant characteristics of Arabidopsis (A-D) and maize (E) in tungen bei Arabidopsis (A-D) und Mais (E). (A) Arabidopsis thaliana-Pflanze automated phenotyping systems. (A) Arabidopsis thaliana plant in early gene- in früher generativer Entwicklungsphase (Blühstadium). (B-D) Arabidopsis rative development phase (flowering stage). (B-D) Arabidopsis thaliana plant thaliana-Pflanze in vegetativer Entwicklungsphase (Rosettenstadium); (B) in vegetative development phase (rosette stage); (B) Fluorescence image (Ex- Fluoreszenzbild (Anregung 400-500 nm, Emission: 520-750 nm; hauptsäch- citation 400-500 nm, Emission: 520-750 nm; mainly detection of red chloro- lich Erfassung der roten Chlorophyllfluoreszenz), C( ) Nahinfrarotbild (1450- phyll fluorescence), C( ) near infrared image (1450-1550 nm; mainly detection 1550 nm; hauptsächlich Erfassung des Wassergehaltes als dunkle Signale), (D) of water content as dark signals), (D) color image in the visible range (390-750 Farbbild im sichtbaren Bereich (390-750 nm). (E) Population genetisch diver- nm). (E) Population of genetically diverse maize plants in automated pheno- ser Maispflanzen in automatisierter Phänotypisierungsanlage (integriertes typing installation (integrated cultivation, transport, and imaging system). Kultivierungs-, Transport- und Aufnahmesystem).

88 Abteilung Molekulare Genetik Department of Molecular Genetics Leiter: Prof. Dr. Thomas Altmann Head: Prof. Thomas Altmann

Allgemeine Forschungsziele Research Goals Das übergeordnete Thema der Forschungsarbeiten in der Abtei- The greater topic of the research performed in the Department lung Molekulare Genetik ist die Analyse und die Modulation der of Molecular Genetics is the analysis and modulation of Leistungsfähigkeit von Pflanzen, wobei ertragsbezogene physio- plant performance addressing yield-related physiological logische Prozesse im Kontext bestimmter Entwicklungsprozesse processes in the context of certain developmental processes. adressiert werden. Die Arbeiten gliedern sich damit überwie- The work is thus predominantly assigned to the IPK research gend in den IPK-Forschungsschwerpunkt „Integrative Biologie topic “Integrative biology of plant performance” with aspects pflanzlicher Leistungen“ ein, wobei Aspekte der Erforschung of investigating plant genetic diversity receiving increasing genetischer Diversität von Pflanzen steigende Berücksichtigung attention. Both, vegetative growth/biomass production and finden. Sowohl vegetatives Wachstum/Biomasseproduktion heterosis (enhanced performance of crossbreds over their und Heterosis (gesteigerte Leistung von Kreuzungsnachkom- parental inbreds) and generative processes such as germ cell men gegenüber ihren Eltern) als auch generative Prozesse, wie formation, seed development and physiology, and seed yield Keimzellbildung, Samenentwicklung und -physiologie und Sa- are studied in detail. A major goal of the work is the elucidation menertrag, werden intensiv erforscht. Ein wesentliches Ziel der of the regulation of central developmental and metabolic Arbeiten ist die Aufklärung der Regulation zentraler Entwick- processes addressing in particular the roles of transcription lungs- und Stoffwechselprozesse, wobei besonders die Rolle von factors and metabolites, of phytohormones, and of novel/ Transkriptionsfaktoren und Metaboliten, von Phytohormonen complex signals. The research programme is characterised und von neuartigen/komplexen Signalen untersucht wird. Das by integration of work directed towards the elucidation of Forschungsprogramm ist geprägt durch die Integration von basic biological processes and phenomena, the development Arbeiten zur Aufklärung grundlegender biologischer Prozesse and application of novel methods and approaches, and und Phänomene, der Entwicklung und Anwendung neuartiger biotechnological, application-oriented research. Methoden und Forschungsansätze und der biotechnologischen, anwendungsorientierten Forschung. The major areas of research in the department are: – basic research on molecular biology and metabolic Die wesentlichen Forschungsfelder der Abteilung umfassen physiology of plant developmental processes: – Grundlagenforschung im Bereich der Molekularbiologie elucidation of molecular control mechanisms of gamete und Stoffwechselphysiologie pflanzlicher Entwicklungspro- development, early embryogenesis, and apomixis zesse: (identification and characterisation of (transcriptional) aufklärung molekularer Kontrollmechanismen der Game- regulators); tenentwicklung, der frühen Embryogenese und der Apomi- analysis of genetic causes and molecular mechanisms xis (Identifizierung und Charakterisierung von (transkriptio- of metabolic performance variation under optimal and nellen) Regulatoren); suboptimal environmental conditions (vegetative growth analyse genetischer Ursachen und molekularer Mechanis- and biomass accumulation, heterosis; seed development men der Variation von Stoffwechselleistungen unter gün- and storage compound accumulation); stigen und ungünstigen Umweltbedingungen (vegetatives – biotechnological, application-oriented research: Wachstum und Biomasseakkumulation, Heterosis; Samen- „Phyto-F(Ph)arming“ (production of novel products for entwicklung und Speicherstoffakkumulation); pharmaceutical or industrial applications in plants); – biotechnologische, anwendungsorientierte Forschung: Development of hybrid technologies (procedures for „Phyto-F(Ph)arming“ (Produktion neuartiger pflanzlicher efficient production of hybrid seeds); Inhaltsstoffe für pharmazeutische und industrielle An- Yield, yield stability and quality of seeds and their wendungen); Entwicklung von Hybridtechnologien (Ver- constituents; fahren zur effizienten Erzeugung von Hybridsaatgut); – Development and application of bioinformatic procedures: Ertrag, Ertragsstabilität und Qualität von Samen und deren integrative bioinformatics and network analysis (data Inhaltsstoffen; representation and integration, visualisation and exploration, – entwicklung und Anwendung bioinformatischer Verfahren: analysis and simulation); integrative Bioinformatik und Netzwerkanalyse (Datenre- DNA motif identification, RNA and protein expression data präsentation und –integration, Visualisierung und Explora- analysis; image analysis for automated phenotyping; tion, Analyse und Simulation); and identifizierung von DNA-Motiven, Analyse von RNA- und – experimental resource-, technology-, and method Proteinexpressionsdaten; development (e.g. microsampling and analysis, NMR-based bildanalyse für automatisierte Phänotypisierung; localisation and quantification of content substances, GC- MS- and LC-MS-based metabolite analytics, microsensorics,

89 Abteilung Molekulare Genetik/Department of Molecular Genetics

und immunological techniques, nucleic acids analytics and – entwicklung experimenteller Ressourcen, Technologien und next generation sequencing, automated non-invasive Methoden (u.a. Mikroprobennahme und -analyse, NMR- phenotyping). basierte Inhaltsstofflokalisierung und -quantifizierung, GC- MS- und LC-MS-basierte Metabolitanalytik, Mikrosensorik, Central goal of the research is to gain a deeper understanding of immunologische Verfahren, Nukleinsäureanalytik und -se- the control and the regulation of plant growth and development quenzierung neuester Generation, automatisierte nicht- with respect to vegetative and generative performance by invasive Phänotypisierung). using an integrative and systems-oriented approach and to transfer results of basic research into application-oriented Zentrales Ziel der Forschung ist es, durch integrative und sys- investigations. temorientierte Analysen ein tieferes Verständnis der Kontrolle und Regulation pflanzlicher Wachstums- und Entwicklungs- prozesse in Bezug auf vegetative und generative Leistungen Developments in 2010 and 2011 von Pflanzen zu erlangen und Grundlagenerkenntnisse in an- In the reporting period, the research groups Image Analysis wendungsorientierte Untersuchungen einzubringen. (since 1 May 2010; head Dr. C. Klukas) and Stress Genomics (since 1 November 2010; head Dr. N. Sreenivasulu) have been newly established. Both are junior research groups, the Stress Entwicklung im Berichtszeitraum Genomics group is part of the Interdisciplinary Center of Crop Im Berichtszeitraum wurden die Arbeitsgruppen „Bildanalyse“ Plant Research (IZN) of the Martin Luther University Halle- (zum 1. Mai 2010; Leiter Dr. C. Klukas) und Stress-Genomik (zum Wittenberg and co-operating non-university institutions. The 1. November 2010; Leiter Dr. N. Sreenivasulu) neu eingerichtet. head of the Data Inspection research group changed (since In beiden Fällen handelt es sich um Nachwuchswissenschaftler- 1 February 2010; head Dr. S. Friedel) and the research group gruppen. Die Arbeitsgruppe Stress-Genomik ist Teil des Inter- Expression Mapping (head Dr. L. Altschmied till 30 September disziplinären Zentrums für Nutzpflanzenforschung (IZN) der 2011) has been dissolved and has been integrated as the Martin-Luther-Universität Halle-Wittenberg und kooperieren- infrastructure unit Nucleic Acids Analytics into the research der außeruniversitärer Einrichtungen. Es wechselte die Leitung group Heterosis. Within this group, the project areas “Natural der Arbeitsgruppe Dateninspektion (seit 1. Februar 2010; Dr. Variation and Heterosis of Plant Growth and Metabolism” and S. Friedel) und es wurde die Arbeitsgruppe „Expressionskar- “Lipid Metabolism and Oil Storage” are headed by Dr. R. Meyer tierung“ (Leiter Dr. L. Altschmied bis 30. September 2011) auf- and Prof. T. Altmann as well as Dr. L. Borisjuk, respectively. gelöst und als Infrastruktureinheit Nukleinsäureanalytik in die Arbeitsgruppe „Heterosis“ integriert. Innerhalb dieser Arbeits- During the reporting period, very substantial investments were gruppe werden die Projektbereiche „Natürliche Variation und made into the setup of automated phenotyping installations Heterosis von Pflanzenwachstum und -stoffwechsel“ sowie „Li- (LemnaTec systems for small plants, e. g. Arabidopsis, Boechera, pidstoffwechsel und Ölspeicherung“ von Dr. R. Meyer und Prof. and for large plants such as maize or sorghum), in which Dr. T. Altmann bzw. von Dr. L. Borisjuk geleitet. several hundred to thousand plants can be cultivated under controlled environmental conditions and their features can be Sehr wesentliche Investitionen flossen in den Berichtsjahren in monitored regularly using different digital camera systems (for den Aufbau automatisierter Phänotypisierungsanlagen (Lem- different spectral ranges). Furthermore, a new high-throughput naTec-Systeme für kleine Pflanzen, z.B. Arabidopsis, Boechera, sequencer has been installed that allows the generation of und für große Pflanzen, z.B. Mais, Hirse), in denen mehrere hun- up to 6 billion sequence reads of approx. 100 nt length per dert bis tausend Pflanzen unter kontrollierten Umweltbedin- machine run. gungen kultiviert werden können und ihre Eigenschaften re- gelmäßig mittels verschiedener elektronischer Kamerasysteme In the following, as it has been the case in previous reports, (für unterschiedliche Spektralbereiche) erfasst werden können. a selection of major results achieved in the reporting period Außerdem wurde eine neue Hochdurchsatz-Sequenzierein- are briefly summarised. They are presented in order of the richtung installiert, die es ermöglicht, bis zu 6 Mrd. Sequenzab- aforementioned research areas. More detailed documentation schnitte von ca. 100 nt Länge pro Gerätelauf zu sequenzieren. of results along with references can be found in the individual reports of the ten research groups of the department, that were Im Folgenden werden, wie in vergangenen Berichten, einige active during the reporting period. wesentliche im Berichtszeitraum erzielte Forschungsergeb- Molecular biology and metabolic physiology of plant nisse aufgeführt. Sie sind hier nach den oben genannten For- developmental processes schungsfeldern gegliedert dargestellt. Detaillierte Erläuterun- – elucidation of genetic and epigenetic mechanisms of gen der Ergebnisse mit Publikationshinweisen finden sich in gamete development, early embryogenesis, and apomixis: den einzelnen Berichten der zehn Arbeitsgruppen der Abtei- important advances were made with respect to the lung, die während des Berichtszeitraumes bestanden. identification and characterisation of the apospory locus HAPPY (a genomic region conditioning embryo formation from unfertilised cells) in Hypericum perforatum (St. John´s

90 Molekularbiologie und Stoffwechselphysiologie pflanzlicher wort). This involved the isolation of sexual and asexual alleles Entwicklungsprozesse and it was shown that the aposporic allele has a complex – aufklärung genetischer und epigenetischer Mechanismen composition with multiple und in part disrupted gene copies der Gametenentwicklung, der frühen Embryogenese und and inserted transposable elements. Further analysis of der Apomixis: genes that exhibit egg cell-specific expression in wheat and entscheidende Fortschritte gelangen in der Identifizierung Arabidopsis resulted in the demonstration that misexpression und Charakterisierung des Aposporie-Locus HAPPY (einer of members of the RKD family of plant transcription factors genomischen Region, die Embryobildung aus unbefruch- in sporophytic cells results in their re-programming and their teten Zellen verursacht) von Hypericum perforatum (Echtes adoption of aspects of egg cell identity. Using the ChIP-Chip Johanniskraut). Dabei wurden sowohl sexuelle als auch method and transcriptome analyses, target genes of central apospore Allele isoliert und es wurde gezeigt, dass das apo- regulators of gene expression during embryogenesis (LEC1, spore Allel eine komplexe Zusammensetzung mit mehrfa- ABI3) were identified. These data gave indications of novel chen z.T. zerstörten Genkopien und inserierten Transposons phytohormone-mediated functions of LEC1 and of the ABI3- aufweist. Die weitere Untersuchung von Genen mit Eizell- regulon represented by 98 genes. A further addressed topic spezifischer Expression in Weizen und Arabidopsis führte concerns the characterisation of the plant RETINOBLASTOMA zu dem Nachweis, dass Missexpression von Mitgliedern homolog (RBR) that is involved in gamete cell fate control der RKD-Familie pflanzlicher Transkriptionsfaktoren in spo- as a key component in the regulation of DNA and histone rophytischen Zellen zu deren Reprogrammierung und der methylation. Annahme von Aspekten der Eizellidentität führt. Mit Hilfe – analysis of genetic causes and molecular mechanisms of der ChIP-Chip-Methodik und der Transkriptomanalyse wur- metabolic performance variation under optimal and subop- den Zielgene von zentralen Regulatoren der Genexpression timal environmental conditions: während der Embryogenese (LEC1, ABI3) identifiziert. Diese Vegetative growth and biomass accumulation, heterosis: Daten lieferten Hinweise auf neue Phytohormon-vermittelte Through analysis of recombinant inbred lines (RILs) and Funktionen von LEC1 und auf das durch 98 Gene repräsen- introgression lines (ILs) of Arabidopsis thaliana and their tierte ABI3-Regulon. Ein weiterer Themenkomplex betrifft test crosses, the identification of 10 TQ L regions for biomass die Charakterisierung des pflanzlichen RETINOBLASTOMA- heterosis was achieved. With fine mapping of one of these Homologs (RBR), das als Schlüsselkomponente in die Regu- QTL and its delineation to approx. 45 kb, major steps lation von DNA- und Histon-Methylierung bei der Kontrolle were made towards elucidation of the genome variation des Zellschicksals von Gameten mitwirkt. causing heterosis and the molecular mechanisms resulting – analyse genetischer Ursachen und molekularer Mechanis- in heterosis expression. Furthermore, candidate genes of men der Variation von Stoffwechselleistungen unter günsti- metabolite QTL were identified, including a gene encoding gen und ungünstigen Umweltbedingungen: a second fumarase in Arabidopsis thaliana (FUM2) the alleles Vegetatives Wachstum und Biomasseakkumulation, Hetero- of which exhibit very substantial differences in expression. sis: Mit Hilfe der Analyse von Rekombinanten Inzuchtlinien Important information on the relations of enzyme activities, (RILs) und Introgressionslinien (ILs) von Arabidopsis thaliana metabolite contents, and growth as well as genome- sowie deren Testkreuzungsnachkommen gelang die Identi- wide DNA variation and its connection with growth and fizierung von 10 TQ L-Regionen für Biomasse-Heterosis. Mit metabolism features were achieved by investigation der Feinkartierung eines dieser QTL und dessen Eingren- of the natural diversity represented by the various wild zung auf ca. 45 kb gelangen entscheidende Schritte zur isolates of Arabidopsis (accessions). Very extensive genetic, Aufklärung der für Heterosis ursächlichen Erbgutvariation phenotypic, and molecular/biochemical characterisation of und der zur Heterosis-Ausprägung führenden molekula- maize diversity collections resulted in clear indications of ren Mechanismen. Weiterhin wurden Kandidatengene für the predictive power of genetic and metabolic markers on Metabolit-QTL identifiziert, darunter ein Gen für eine zwei- inbred and hybrid performance and the degree of heterosis te Fumarase in Arabidopsis thaliana (FUM2), dessen Allele as shown before for Arabidopsis. These findings point to a erhebliche Expressionsunterschiede aufweisen. Wichtige strong potential for future applications in maize breeding. Informationen über die Beziehungen von Enzymaktiviäten, Seed development and storage compound accumulation: The Metabolitgehalten und Wachstum sowie über genomweite analysis of barley mutants affected in starch accumulation DNA-Variation und deren Zusammenhang mit Wachstums- and in the regulation of the contents of the phytohormone und Stoffwechseleigenschaften wurden durch Untersu- abscisic acid (ABA) gave hints on overlaps of metabolic chungen der in vielfältigen Wildformen von Arabidopsis and hormonal regulation during seed development and thaliana (Akzessionen) repräsentierten natürlichen Diver- filling. Results of comparative analyses using transgenic sität erlangt. Sehr umfangreiche genetische, phänotypi- pea plants confirmed the importance of ABA content and sche und molekulare/biochemische Charakterisierung von sensitivity variation for the regulation of developmental Maisdiversitätskollektionen lieferten klare Hinweise auf die phase transitions and of storage metabolism in seeds. More Vorhersagekraft von genetischen und metabolischen Mar- in-depth information on signalling cascades and regulatory kern für die Eigenleistung, Hybridleistung und das Ausmaß interactions between different tissues were gained via der Heterosis, wie zuvor bei Arabidopsis beobachtet. Diese detailed metabolite and transcript analyses of isolated

91 Abteilung Molekulare Genetik/Department of Molecular Genetics

Erkenntnisse deuten auf ein wertvolles Potenzial für zukünf- tissue samples from seeds of different developmental tige Anwendungen in der Maiszüchtung hin. stages. In addition to the previously characterised cysteine- Samenentwicklung und Speicherstoffakkumulation: Die rich JEKYLL-protein in barley, vacuolar processing enzymes Analyse von Gerstenmutanten mit Störungen in der Stär- (VPEs) in barley and small cysteine-rich proteins in pea were keakkumulation und in der Regulation des Gehaltes des identified as further putative factors with function in cellular Phytohormons Abscisinsäure (ABA) lieferten Hinweise auf disintegration processes, which play important roles in Überlagerung metabolischer und hormoneller Regulation the interaction between maternal and filial tissues and the während der Samenentwicklung und -füllung. Vergleichen- nutrient supply to storage tissues. Finally, it was confirmed de Analysen unter Verwendung transgener Erbsenpflanzen that enhanced sucrose import capacity specifically achieved bestätigten die Bedeutung der Variation von ABA-Gehalten in transgenic wheat plants provokes a stimulation of storage und -Sensitivität für die Regulation des Übergangs von protein synthesis and an increase in grain weight. Entwicklungsphasen und des Speicherstoffwechsels in Sa- analysis of a population of barley introgression lines carrying men. Vertiefende Informationen über Signalkaskaden und wild barley genome segments resulted in the identification of regulatorische Wechselwirkungen zwischen verschiedenen a total of 28 major QTL for agronomic performance parameters Geweben wurden durch eingehende Metabolit- und Tran- expressed under terminal drought stress conditions. The skriptuntersuchungen isolierter Gewebeproben aus Samen importance of ABA for yield stability under these conditions verschiedener Entwicklungsstadien gewonnen. Als weitere shown by investigations of contrasting lines and deepened putative Faktoren mit wichtigen Funktionen bei zellulären through detailed analyses of ABA synthesis, degradation, Abbauprozessen, die im Zusammenwirken zwischen mater- and signal transduction in different organs and substantial nalen und filialen Geweben und der Versorgung von Spei- differences in ABA-homeostasis and responses were verified chergeweben eine wichtige Rolle spielen, wurden neben for tolerant vs. sensitive lines. These results were confirmed dem zuvor charakterisierten Cystein-reichen JEKYLL-Protein through investigation of transgenic plants with targeted der Gerste vakuoläre Prozessierungsenzyme (VPEs) in Gers- alterations of ABA accumulation or sensitivity. te und kleine Cystein-reiche Proteine in Erbse identifiziert. using further developed (NMR-based) imaging techniques Schließlich wurde nachgewiesen, dass die in transgenen for the 3-dimensional topology of seeds and seed contents Weizenpflanzen erzielte gesteigerte Saccharoseaufnahme- as well as microsampling and analysis methods, crucial kapazität zu einer Stimulierung der Speicherproteinsynthese novel insights into spatial and temporal progression of führt und eine Erhöhung des Korngewichtes bewirkt. Mit Hil- import, allocation, and conversion of metabolites and the fe der Analyse einer Population von Gerstenintrogressions- accumulation of storage compounds were gained. Novel

linien, die Wildgerstengenomsegmente enthalten, wurden O2 and NO sensors were successfully applied to analyse the insgesamt 28 Haupt-QTL für agronomische Leistungspara- concentrations of these gases in different plant tissues. meter unter terminalen Trockenstressbedingungen identifiziert. Die durch detaillierte Analyse kontrastierender Linien Biotechnological, application-oriented research belegte Bedeutung von Abscisinsäure (ABA) für die Ertrags- – „Phyto-F(Ph)arming“: stabilität unter terminalen Trockenstressbedingungen wur- in addition to the production of various individual spider de durch eingehende Untersuchungen von ABA-Synthese, silk proteins in plants using specially designed expression -Abbau und -Signalübertragung in verschiedenen Organen vectors, the development and application of a novel vertieft und deutliche Unterschiede in ABA-Homöostase concept for the production of multimeric spider silk proteins und -Reaktionen zwischen toleranten und sensitiven Linien in plants was achieved. Isolated and purified multimers were nachgewiesen. Diese Ergebnisse wurden durch Analysen characterised using electron microscopy. Furthermore, transgener Pflanzen mit gezielter Veränderung von ABA- different antigens of the avian flue virus(H5N1) partly Akkumulation und -Sensitivität bestätigt. Mit Hilfe weiter- in form of fusions to ELP (elastin-like polypeptide) were entwickelter (NMR-basierter) bildgebender Verfahren für produced in plants. The mITC technology was developed for die dreidimensionale Topologie von Samen und Samenin- improved purification of ELPylated proteins and was used haltsstoffen sowie Methoden der Mikroprobennahme und to generate hemagglutinin and neuraminidase in increased -analytik wurden entscheidende neue Erkenntnisse über die quality. Produced hemagglutinin trimers and virus-like räumlichen und zeitlichen Verläufe von Import, Verteilung particles were successfully used for immunisation of mice. und Konversion von Metaboliten und die Akkumulation von Finally novel specific antibodies against tumor necrosis Speichersubstanzen in Samen gewonnen. Neuartige Sen- factor alpha could be isolated.

soren für O2 und NO wurden erfolgreich für die Analyse der – Development of hybrid technologies: Konzentrationen dieser Gase in verschiedenen pflanzlichen as an alternative to chemical pollen inactivation, a highly Geweben eingesetzt. effective system for the production of hybrid plant seeds that is based on a split-gene approach has been further Biotechnologische, anwendungsorientierte Forschung developed. The two protein fragments produced in the – „Phyto-F(Ph)arming“: female parental line from the two parts of the gene cause neben verschiedenen individuellen Spinnenseidenproteinen, pollen ablation and thus male sterility. As always only one die mit Hilfe besonders gestalteter Expressionsvektoren in of the two partial genes is transmitted to the progeny

92 Pflanzen produziert wurden, gelang es, ein neuartiges Kon- produced through cross-pollination, which on its own is zept für die Produktion multimerer Spinnenseidenproteine inactive, the produced hybrids are fully fertile. During the in Pflanzen zu entwickeln und einzusetzen. Isolierte und ge- reporting period the expression level and thereby the reinigte Multimere wurden mittels Elektronenmikroskopie penetrance of the induced sterility was vitally improved. In untersucht. Ferner wurden verschiedene Antigene des Vo- addition, procedures for the identification of good pollen gelgrippevirus (H5N1) u. a. als Fusionen mit ELP (Elastin-ähn- donors and for the generation of doubled haploids in wheat liches Polypeptid) in Pflanzen produziert. Für die verbesserte were developed. Reinigung ELPylierter Proteine wurde die mITC-Technologie entwickelt und für die Erzeugung von Hämagglutinin und Development and application of bioinformatic procedures Neuraminidase in erhöhter Qualität eingesetzt. Produzier- Bioinformatics methods are indispensible components te Hämagglutinin-Trimere und Virus-ähnliche Partikel wur- of the technological repertoire of modern plant biology den erfolgreich zur Immunisierung von Mäusen eingesetzt. research. According to their generic nature, the developed Schließlich konnten neue spezifische Antikörper gegen and implemented procedures are used across departments Tumornekrosisfaktor alpha isoliert werden. in numerous projects. Important contributions of groups of – entwicklung von Hybridtechnologien: the Molecular Genetics department include: als Alternative zu chemischer Pollenabtötung wurde ein – co-ordination of bioinformatics research at IPK hoch effizientes Verfahren zur Erzeugung von Hybridsaat- (Prof. F. Schreiber). gut bei Pflanzen weiterentwickelt, das auf einem „geteilten – integrative bioinformatics and network analysis: Gen“ beruht. Die (Teil-)Proteine, die in den als mütterliche the open source system VANTED for the analysis of Elternlinie herangezogenen Pflanzen von den zwei Teilen experimental data in the context of biological networks dieses Gens produziert werden, führen zum Absterben has been further developed (currently available as version der Pollen und damit zu männlicher Sterilität. An die durch 2.0) and has been expanded through addition of novel Fremdbefruchtung erzeugten Nachkommen wird jedoch algorithms for facilitated integration and analysis of data (e.g. nur jeweils eines der zwei Teilgene vererbt, die für sich infor integration of metabolic or regulatory knowledge or for aktiv sind. Daher sind die erzeugten Hybriden vollständig recognition of overrepresented expressed genes of certain fruchtbar. Im Berichtszeitraum konnte die Expressionsstärke pathways) and has been used in various projects. In addition, und damit die Penetranz der ausgelösten Sterilität entschei- several add-ons (Hive, SBGN-ED, CentiLib, FluxMap) were dend erhöht werden. Darüber hinaus wurden Verfahren zur developed which offer new functionalities. Furthermore, Identifizierung guter Pollenspender und zur Erzeugung von development of the MetaCrop information system for Doppelhaploiden bei Weizen entwickelt. metabolic pathways has been continued and version 2.0 has been released. The extended contents of MetaCrop Entwicklung und Anwendung bioinformatischer Verfahren now cover information on approx. 62 metabolic pathways Als generische Verfahren gehören bioinformatische Metho- derived from more than 1800 publications. These data are den zum unverzichtbaren Repertoire moderner pflanzenbio- the basis of various stoichiometric metabolic models that logischer Forschung und finden abteilungsübergreifend An- have been developed and analysed. The DBE2 information wendung in vielfältigen Projekten. Wichtige Beiträge von Ar- system for the management of biological experiment data beitsgruppen der Abteilung Molekulare Genetik umfassen: and a system to display and handle biological networks – koordination der Bioinformatikarbeiten am IPK based on an international convention (SBGN) were further (Prof. Dr. F. Schreiber) developed. Finally, an established image analysis pipeline for – integrative Bioinformatik und Netzwerkanalyse: the analysis of data produced by automated phenotyping Das open source System VANTED für die Analyse experimen- facilities has been further developed and various novel teller Daten im Kontext biologischer Netzwerke wurde wei- algorithms for the structural network analysis and display as terentwickelt (aktuell in Version 2.0 verfügbar) und um neue well as efficient algorithms using parallel architectures were Algorithmen für die erleichterte Integration, Exploration und developed, implemented, and used. Analyse von Daten (u.a. zur Integration von metabolischen – Processing, visualisation, and interpretation of high- und regulatorischen Kenntnissen oder zur Erkennung überre- dimensional biological data: präsentierter exprimierter Gene bestimmter Stoffwechselwe- applying machine learning techniques and Hidden Markov ge) erweitert und in vielfältigen Projekten eingesetzt. Zudem Models, array-CGH and ChIP-Chip data sets were used for wurden diverse add-ons (Hive, SBGN-ED, CentiLib, FluxMap) comparative genome analysis and epigenetic chromatin entwickelt, die neue Funktionalitäten bereitstellen. Ferner modification investigation of diverse Arabidopsis thaliana wurde die Weiterentwicklung des MetaCrop Informations- accessions and hybrids thereof. They were expanded systems für Stoffwechselwege fortgeführt und die Version 2.0 towards identification of newly inserted transposons freigegeben. Mit der Erweiterung der Inhalte von MetaCrop through analysis of the DNA methylation status. A novel sind nun Informationen zu ca. 62 Stoffwechselwegen aus tool for de novo identification of DNA motifs (Dispom) mehr als 1800 Publikationen verfügbar. Diese Daten stellen has been developed for transcription factor binding site die Grundlage für diverse stoichiometrische Stoffwechsel- recognition and a new motif was found in auxin-regulated modelle dar, die entwickelt und analysiert wurden. Das DBE2 promotors. Arabidopsis gene regulation networks affected

93 Abteilung Molekulare Genetik/Department of Molecular Genetics

Informationssystem für das Management biologischer Ex- by overexpression of AtHb1 were reconstructed through perimentdaten und ein auf internationalen Konventionen reverse engineering. Finally, novel procedures based (SBGN) beruhendes System zur Darstellung und Handhabung on modern probabilistic models were used to evaluate biologischer Netzwerke wurden weiterentwickelt. Schließlich historic data on Genebank accessions and supported the wurde eine erstellte Bildanalysepipeline für die Verarbeitung identification of accessions with desired features or the von Daten aus automatisierten Phänotypisierungsanlagen selection of contrasting accessions. weiterentwickelt und diverse neue Algorithmen für die struk- – Development of storage and analysis systems for high- turelle Netzwerkanalyse und die Netzwerkdarstellung sowie throughput image data: effizienteA lgorithmen unter Verwendung paralleler Architek- a Java-based system (IAP) for efficient handling and analysis turen entwickelt, implementiert und angewendet. of image data generated by multiple camera systems of – Prozessierung, Visualisierung und Interpretation hoch-di- the automated phenotyping systems has been developed mensionaler biologischer Daten: that offers various functionalities to users: Processing Mit Hilfe von Verfahren des maschinellen Lernens und unter of images and further experimental data, data transfer Einsatz von Hidden Markov-Modellen wurden Array-CGH- for long-term archiving, download of data for individual und ChIP-Chip-Datensätze zur vergleichenden Genomana- analyses, and performance of automated image analysis lyse einschließlich epigenetischer Chromatinmodifikationen for experimental data. Within IAP several image processing verschiedener Arabidopsis thaliana-Akzessionen und deren and analysis procedures were implemented that after pre- Hybriden eingesetzt. Diese wurden für die Identifizierung processing support the extraction of information on plant neu inserierter Transposons unter Analyse des DNA-Methy- features (including height, width, projected area, estimated lierungsstatus erweitert. Ein neues Werkzeug für die de-novo- biomass, etc.). Results of data analyses can be exported Identifizierung von DNA-Motiven (Dispom) wurde für die as data tables or as automatically generated report files. Erkennung von Transkriptionsfaktorbindestellen entwickelt und damit ein neues Motiv in Promotoren Auxin-regulierter The following group reports provide more detailed insights into Gene gefunden. Mittels reverse engineering wurden Genre- the research and the achieved results of the individual research gulationsnetzwerke rekonstruiert, die bei Arabidopsis Ver- groups of the department. änderungen durch die Überexpression von AtHb1 erfahren. Schließlich wurden neue Verfahrensweisen unter Nutzung moderner probabilistischer Methoden für die Auswertung Thomas Altmann, November 2011 historischer Daten zu Genbank-Akzessionen entwickelt und für die Identifizierung von Akzessionen mit gesuchten Ei- genschaften bzw. die Kennzeichnung kontrastierender Ak- zessionen eingesetzt. – entwicklung von Systemen für die Speicherung und Analyse von Hochdurchsatz-Bilddaten: zur effektiven Handhabung und Analyse von Bilddaten, die von multiplen Kamerasystemem in automatisierten Phänotypisierungsanlagen geliefert werden, wurde ein Java-basiertes Informationssystem (IAP) eingerichtet, das Nutzern verschiedene Funktionalitäten bereitstellt: Analyse von Bilddaten und weiteren experimentellen Daten, Daten- transfer für langfristige Archivierung, Herunterladen von Daten für eigene Analysen und Ausführen automatisierter Bildanalyseprozeduren für Experimentdaten. Innerhalb von IAP wurde eine Reihe von Bildverarbeitungs- und Analyse- funktionen eingerichtet, die es nach Vorverarbeitung erlauben, Informationen über Pflanzeneigenschaften (u.a. Höhe, Breite, projizierte Fläche, geschätzte Biomasse etc.) zu extra- hieren. Ergebnisse der Datenanalysen können in Form von Datentabellen und als automatisch erzeugte Berichtsdatei- en exportiert werden.

Die folgenden Berichte der Arbeitsgruppen geben detaillierte- re Einblicke in die Forschungsarbeiten und die erzielten Ergeb- nisse der einzelnen Arbeitsgruppen der Abteilung.

Thomas Altmann, November 2011

94 Research Group: Heterosis Head: Prof. Thomas Altmann

Scientists – Lipid metabolism and sucrose allocation in seeds (mainly rapeseed, oat, and barley) is investigated using topographical IPK financed biophysical, biochemical, and molecular analysis procedures Altschmied, Lothar, Dr. (since 01.08.2011) to uncover determinants and limiting factors of yield. Borisjuk, Lioudmilla, Dr. Meyer, Rhonda, Dr. Radchuk, Ruslana, Dr. (0.50, 01.03.-31.07.2011; since 01.08.2011) Research Report Riewe, David, Dr. Schiebold, Silke (0.50, 01.08.-31.10.2010) Project area Analysis of Growth and Metabolism during Vegetative Development Grant Positions Arana Ceballos, Fernando, Dr. (DFG, since 15.07.2010) Identification and fine mapping of QTL for biomass heterosis Ernst, Michaela, Dr. (BMBF, till 31.08.2010) in Arabidopsis thaliana accessions Col-0 and C24 Fuchs, Johannes (0.50 Industry) Analysis of previously established recombinant inbred line (RIL; Gryczka, Corina, Dr. (BMBF, since 01.09.2010) Törjek et al. 2006) and introgression line (IL; Törjek et al. 2008) Heinzel, Nicolas, Dr. (Industry) populations of the Arabidopsis accessions Col-0 and C24 jointly Jasik, Jan, Dr. (Industry, till 31.12.2010) with their respective testcrosses (TCs) for dry matter yield and Kalladan, Rajesh (0.50 BMBF, till 30.09.2010) leaf area during early vegetative development resulted in the Melkus, Gerd (BMBF, till 30.06.2010; Industry 01.07.-30.09.2010) identification of genomic regions, that condition heterosis for Müller, Margarete, Dr. (BMBF, till 30.04.2011) vegetative growth/biomass accumulation (see Fig. 34, p. 96) Muraya, Moses Mahugu, Dr. (BMBF) (Meyer et al. 2010). Using data of RILs/RIL-TCs and ILs/IL-TCs, a Radchuk, Ruslana, Dr. (0.50 Industry, 01.04.-31.07.2011) total of ten genomic regions responsible for early stage biomass Rolletschek, Hardy, Dr. (Industry) heterosis were identified that individually explained between Rosso, Mario, Dr. (BMBF) 2.4 % and 15.7 % of the observed phenotypic variation. Schiebold, Silke (0.50 Industry, till 31.07.2010; 01.11.2010- Individual heterotic QTL mostly exhibited overdominant 14.04.2011) gene action. Furthermore, the obtained results suggest Seyfarth, Monique (0.50 Saxony-Anhalt) that biomass heterosis in this Arabidopsis cross involves a Tschiersch, Henning, Dr. (0.50 Industry, till 31.07.2010; BMBF, combination of dominance, overdominance and epistasis. since 01.08.2010) Three heterotic biomass (hb)QTL located on chromosomes 1, Weigelt, Kathleen (BMBF, till 31.03.2011) 3, and 4 were chosen for fine mapping and detailed molecular characterisation. Using genotype and phenotype information Visiting Scientists/Scholars of approx. 600 individuals of IL families (ILFs) segregating for Ernst, Michaela, Dr. (self-financed, 01.09.2010-31.03.2011; genomic segments spanning the hbQTL on chromosome 4, since 25.11.2011) the QTL region was confined to a region of approx. 45 kb Jeon, Hea-Jung (Korean government, since 27.09.2010) harbouring 14 annotated genes (R. Schmidt, R. Meyer, Koohi-Dehkordi, Mehrana (Iranian Ministry of Science, Research unpublished data). Analysis of corresponding subILs and their and Technology, 10.01.-31.08.2010; 01.12.2010-30.09.2011) testcrosses confirmed heterosis to be caused by heterozygosity of the fine-mapped region (R. Schmidt, K. Weigelt, R. Meyer, unpublished data). Characterisation of T-DNA k.o. mutants of genes in the support interval and testcrosses thereof as Goals well as generation of transgenic plants transformed with segments of this region derived from the opposite genotype With the major aim of identifying factors controlling plant is ongoing (R. Meyer, D. Riewe, B. Ebert, K. Weigelt, H.-J. Jeon, performance characteristics, two complementary research unpublished data). The hbQTL located on chromosomes 1 areas are addressed within the group: and 3 are fine-mapped via phenotyping and genotyping of – Genetic/genomic approaches are followed to identify growth RIL-derived segregating heterogeneous inbred families (HIFs) and metabolism controlling factors of vegetative development and ILFs (K. Weigelt, F. Arana-Ceballos, unpublished data). The (addressing mainly Arabidopsis and maize). Major emphasis is same strategy is followed for fine mapping of another (non- given to the discovery of genetic and biological markers for heterotic) biomass QTL located on chromosome 3, suspected the prediction of (vegetative) biomass accumulation and to to be involved in cross-talk between metabolism and growth the identification of loci contributing to heterosis control (M. Seyfarth, unpublished data). and

95 Abteilung Molekulare Genetik/Department of Molecular Genetics

Fig. 34 Localisation of heterotic and per se QTL on Col-0/C24 genetic map. DW – dry matter yield, LA – leaf area, RGR – relative growth rate, -C24 – mid-parent-heterosis in cross C24 × RIL, -Col – mid-parent-heterosis in cross Col-0 × RIL, DW-IL – hbQTL detected in IL. (Meyer et al. 2010)

Characterisation of genes corresponding to metabolite QTL Natural genetic variation of metabolism and growth in Arabi- in Arabidopsis thaliana and further investigation of growth dopsis was further studied in a collection of 129 accessions that – metabolism relations were characterised for activities of 37 enzymes from central me- Based on previous data of metabolite (m)QTL identified through tabolism in addition to metabolite contents and accumulated gas chromatography/mass spectrometry (GC/MS)-based meta- biomass (Sulpice et al. 2010). While highly co-ordinated chang- bolic profiling of the aforementioned RIL and IL populations es were observed for most enzymes and for certain subsets of (Lisec et al. 2008), 30 candidate genes were selected for an metabolites, little correlation was found between enzyme ac- initial analysis according to their proposed enzymatic role re- tivities and metabolites and only weak correlations between in- lated to 15 different metabolites affected by TmQ L. Effects on dividual enzyme activities and biomass. However, the fraction abundance of the corresponding metabolite were observed of total protein allocated to enzymes was positively correlated by T-DNA k.o. mutations of 5 genes that were selected for fur- with biomass and additive to the negative starch-biomass cor- ther investigation using also TILLING mutants and dsRNAi lines relation (Sulpice et al. 2010). A subset of 54 of the accessions subjected to GC/MS analyses (Brotman et al. 2011; D. Riewe, was furthermore subjected to deep genotypic characterisation M. Koohi, M. Pfeiffer, unpublished data). One of the five genes through single feature polymorphism (SFP) analysis using til- (At5g50950) was thus shown to encode a second functional fu- ing arrays of 3 million oligonucleotides (Childs et al. 2010). This marase (FUM2) isoform in Arabidopsis (Brotman et al. 2011). The resulted in the detection of more than 1 million SFPs. A total of Col-0 and C24 alleles of this gene (corresponding to the fumar- 198 significant associations between SFPs of metabolic genes ase mQTL alleles) were shown to differ approx. 16-fold in the and 9 metabolic and growth-related traits were observed and level of their expression, which very likely causes a substantial five significant selective sweep regions were detected, one of difference in FUM2 activity and thus fumarate accumulation which is significantly associated with a metabolic trait C( hilds (Brotman et al. 2011). et al. 2010).

96 Fig. 35 Visualisation of 13C-sugar allocation within the caryopsis using double resonant 13C/1H NMR. A proton NMR reference image is shown at the left. Right are selected 2D colour coded maps demonstrating the distribution of 13C-sugar during 12 h long monitoring experiment (Melkus et al. 2011).

Characterisation of diverse maize inbred line collections In vivo analysis of storage and In the frame of the GABI-ENERGY and PLANT-KBBE CornFed metabolite distribution in plants co-operation projects (jointly with A. Melchinger, University of The NMR-based lipid mapping tool was applied for investiga- Hohenheim; L. Willmitzer and M. Stitt, MPI-MP, Golm; J. Selbig, tion of lipid metabolism in oat, and resulted in defining of the Potsdam University; A. Charcosset, INRA Moulon; D. Brunel, cofactome as a promising target for biotechnological improve- CNG Evry; C. Schön, TU München; M. Ouzunova, KWS Einbeck; ments of seed. (Hayden et al. 2011). This work represents an and their co-workers) detailed genetic and phenotypic ongoing cooperation with UC Davis/USA (Prof. K. Dehesh). To characterisation of diverse collections of maize inbred lines study the distribution and flow of metabolites non-invasively, (`flint´, ´dent´, and `tropical´ panels), DH lines and testcrosses we applied NMR-based approaches. Current methods have thereof is being carried out. These joint efforts have led to the been developed based on 1H/13C-NMR (SysSeed and in col- accumulation of genotype data based on the maize 50k SNP laboration with University of Würzburg/Prof. P. Jakob). This al- array for a total of more than 1,000 inbred lines and more than lowed for the first time to visualise the allocation of sucrose in 2,200 DH lines (the latter are arranged in a nested association living seeds (Fig. 35, Melkus et al. 2011). Application of this new mapping (NAM) design). In addition to field characterisation of technology to barley seeds as a model visualised the site of 289 dent inbreds and testcrosses with two testers for agronomic primary synthesis of alanine and revealed compartmentation traits and metabolite profiles (Larhlimi et al. submitted; of the starchy endosperm (Rolletschek et al. 2011). We aim to Riedelsheimer et al. submitted), a subset of 92 inbreds and improve this method and widen its application, but this in vivo testcrosses thereof (selected after initial analysis of the entire technology already represents a significant breakthrough in population) has been characterised for vegetative growth seed research with high biotechnological relevance. performance under controlled (greenhouse) conditions, for metabolite composition, and for transcript profiles (M. Ernst, Microsensor-based technology for the investigation of C. Gryczka, S. Friedel, unpublished data). The collected data oxygen, carbon dioxide, and nitric oxide in plants are being used for the demonstration of metabolite and SNP- Planar oxygen sensors are developed, which allow to quantify based prediction of inbred and hybrid performance, as well as oxygen and nitric oxide (NO) on the surface of tissues. Both association mapping of QTL affecting growth and metabolism. oxygen and NO are important signalling molecules involved in dynamic adjustment of seed metabolism to environmental Project area Lipid Metabolism in Oil-storing Seeds and developmental changes (Thiel et al. 2011). A planar sensor for oxygen was successfully applied on different plant species Modelling lipid metabolism using a and tissues (Tschiersch et al. 2011). Sensor development is in systems biology approach progress in cooperation with PreSense GmBH (Regensburg/ Lipid metabolism and seed filling of rape seed were analysed Germany). using histological methods, NMR-based modelling and the lipid imaging tool. The project is in progress and relies on extensive collaboration with partners from University Hannover, Brookhaven National Lab (USA) and Bayer CropScience (Belgium). A novel quantitative assay for evaluation of promoter activity was developed, which integrates laser-microdissection and an enzymatic assay (Jasik et al. 2011). We also developed tools for the analysis of metabolites and storage products in laser microdissected tissues, which are easily combined with gene expression analysis (Schiebold et al. 2011). The bioanalytical tools were also used in other collaborations (e.g. GABI-Grain; SysSeed) and resulted in several publications (Rolletschek et al. 2011; Cornelius et al. 2011; Hayden et al. 2011; Seiler et al. 2011).

97 Abteilung Molekulare Genetik/Department of Molecular Genetics

Publications Mu r a y a , M.M., H. Ha r t w i g , H.H. Ge i g e r , E. Mu t e g i , B. Ka n y e n j i , F. Sa g n a r d , S. De Vi l l i e r s , D. Ki a m b i & H.K. Pa r z i e s : Geographical (The publication with a “*” is based on work that has been patterns of phenotypic diversity and structure of Kenyan carried out when N. Heinzel was at the Max Planck Institute for wild sorghum populations (Sorghum spp.) as an aid to Chemical Ecology Jena). germplasm collection and conservation strategy. Plant Genet. Resour. 8 (2010) 217-224. Peer Reviewed Papers Mu r a y a , M.M., F. Sa g n a r d & H.K. Pa r z i e s : Investigation of recent population bottlenecks in Kenyan wild sorghum populations 2010 (Sorghum bicolor (L.) Moench ssp. verticilliflorum (Steud.) An d o r f , S., J. Se l b i g , T. Al t m a n n , K. Po o s , H. Wi t u c k a -Wa l l & D. De Wet) based on microsatellite diversity and genetic Re p s i l b e r : Enriched partial correlations in genome-wide disequilibria. Genet. Resour. Crop Evol. 57 (2010) 995-1005. gene expression profiles of hybrids (A. thaliana): a systems Mu t e g i , E., F. Sa g n a r d , M. Mu r a y a , B. Ka n y e n j i , B. Ro n o , C. Mw o n g e r a , biological approach towards the molecular basis of heterosis. C. Ma r a n g u , J. Ka m a u , H. Pa r z i e s , S. d e Vi l l i e r s , K. Se m a g n , P.S. Theor. Appl. Genet. 120 (2010) 249-259. Tr a o r é & M. La b u s c h a g n e : Ecogeographical distribution of Be c h t o l d , U., T. La w s o n , J. Me j i a -Ca r r a n z a , R.C. Me y e r , I.R. Br o w n , T. wild, weedy and cultivated Sorghum bicolor (L.) Moench in Al t m a n n , J. To n & P.M. Mu l l i n e a u x : Constitutive salicylic acid Kenya: implications for conservation and crop-to-wild gene defences do not compromise seed yield, drought tolerance flow. Genet. Resour. Crop Evol. 57 (2010) 243-253. and water productivity in the Arabidopsis accession C24. Sreenivasulu , N., L. Bo r i s j u k , B.H. Ju n k e r , H.P. Mo c k , H. Ro l l e t s c h e k , Plant Cell Environ. 33 (2010) 1959-1973. U. Se i f f e r t , W. We s c h k e & U. Wo b u s : Barley grain development Ch i l d s , L.H., H. Wi t u c k a -Wa l l , T. Gü n t h e r , R. Su l p i c e , M. v o n Ko r f f , toward an integrative view. Int. Rev. Cell Mol. Biol. 281 (2010) M. St i t t , D. Wa l t h e r , K.J. Sc h m i d & T. Al t m a n n : Single feature 49-89. polymorphism (SFP)-based selective sweep identification Sreenivasulu , N., V. Ra d c h u k , A. Al a w a d y , L. Bo r i s j u k , D. We i e r , N. and association mapping of growth-related metabolic traits St a r o s k e , J. Fu c h s , O. Mi e r s c h , M. St r i c k e r t , B. Us a d e l , U. Wo b u s , in Arabidopsis thaliana. BMC Genomics 11 (2010) 188. B. Gr i m m , H. We b e r & W. We s c h k e : De-regulation of abscisic acid Geigenberger , P., D. Ri e w e & A.R. Fe r n i e : The central regulation of plant contents causes abnormal endosperm development in the physiology by adenylates. Trends Plant Sci. 15 (2010) 98-105. barley mutant seg8. Plant J. 64 (2010) 589-603. Ho, Q.T., P. Ve r b o v e n , B.E. Ve r l i n d e n , A. Sc h e n k , M.A. De l e l e , H. St e i n f a t h , M., T. Gä r t n e r , J. Li s e c , R.C. Me y e r , T. Al t m a n n , L. Wi l l m i t z e r & Ro l l e t s c h e k , J. Ve r c a m m e n & B.M. Ni c o l a i : Genotype effects on J. Se l b i g : Prediction of hybrid biomass in Arabidopsis thaliana internal gas gradients in apple fruit. J. Exp. Bot. 61 (2010) by selected parental SNP and metabolic markers. Theor. 2745-2755. Appl. Genet. 120 (2010) 239-247. *Ka u r , H., N. He i n z e l , M. Sc h o t t n e r , I.T. Ba l d w i n & I. Ga l i s : R2R3- Su l p i c e , R., S. Tr e n k a m p , M. St e i n f a t h , B. Us a d e l , Y. Gi b o n , H. Wi t u c k a - NaMYB8 regulates the accumulation of phenylpropanoid- Wa l l , E.T. Py l , H. Ts c h o e p , M.C. St e i n h a u s e r , M. Gu e n t h e r , M. polyamine conjugates, which are essential for local and Ho e h n e , J.M. Ro h w e r , T. Al t m a n n , A.R. Fe r n i e & M. St i t t : Network systemic defense against insect herbivores in Nicotiana analysis of enzyme activities and metabolite levels and attenuata. Plant Physiol. 152 (2010) 1731-1747. their relationship to biomass in a large panel of Arabidopsis Ma s s o n n e t , C., D. Vi l e , J. Fa b r e , M.A. Ha n n a h , C. Ca l d a n a , J. Li s e c , accessions. Plant Cell 22 (2010) 2872-2893. G.T.S. Be e m s t e r , R.C. Me y e r , G. Me s s e r l i , J.T. Gr o n l u n d , J. Pe r k o v i c , E. Wi g m o r e , S. Ma y , M.W. Be v a n , C. Me y e r , S. Ru b i o -Di a z , D. We i g e l , 2011 J.L. Mi c o l , V. Bu c h a n a n -Wo l l a s t o n , F. Fi o r a n i , S. Wa l s h , B. Ri n n , W. Bo r i s j u k , L., H. Ro l l e t s c h e k , J. Fu c h s , G. Me l k u s & T. Ne u b e r g e r : Low Gr u i s s e m , P. Hi l s o n , L. He n n i g , L. Wi l l m i t z e r & C. Gr a n i e r : Probing and high field magnetic resonance for in vivo analysis of the reproducibility of leaf growth and molecular phenotypes: seeds. Materials 4 (2011) 1426-1439. A comparison of three Arabidopsis accessions cultivated in Br o t m a n , Y., D. Ri e w e , J. Li s e c , R.C. Me y e r , L. Wi l l m i t z e r & T. Al t m a n n : ten laboratories. Plant Physiol. 152 (2010) 2142-2157. Identification of enzymatic and regulatory genes of plant Me y e r , R.C., B. Ku s t e r e r , J. Li s e c , M. St e i n f a t h , M. Be c h e r , H. Sc h a r r , A.E. metabolism through QTL analysis in Arabidopsis. J. Plant Me l c h i n g e r , J. Se l b i g , U. Sc h u r r , L. Wi l l m i t z e r & T. Al t m a n n : QTL Physiol. 168 (2011) 1387-1394. analysis of early stage heterosis for biomass in Arabidopsis. Co r n e l i u s , S., S. Wi t z , H. Ro l l e t s c h e k & T. Mö h l m a n n : Pyrimidine Theor. Appl. Genet. 120 (2010) 227-237. degradation influences germination seedling growth and production of Arabidopsis seeds. J. Exp. Bot. 62 (2011) 5623- 5632.

98 Hä d r i c h , N., Y. Gi b o n , C. Sc h u d o m a , T. Al t m a n n , J.E. Lu n n & M. St i t t : Sc h i e b o l d , S., H. Ts c h i e r s c h , L. Bo r i s j u k , N. He i n z e l , R. Ra d c h u k & H. Use of TILLING and robotised enzyme assays to generate Ro l l e t s c h e k : A novel procedure for the quantitative analysis an allelic series of Arabidopsis thaliana mutants with altered of metabolites, storage products and transcripts of laser ADP-glucose pyrophosphorylase activity. J. Plant Physiol. microdissected seed tissues of Brassica napus. Plant Methods 168 (2011) 1395-1405. 7 (2011) 19. Ha y d e n , D.M., H. Ro l l e t s c h e k , L. Bo r i s j u k , J. Co r w i n , D.J. Kliebenstein , Se i l e r , C., V.T. Harshavardhan , K. Ra j e s h , P.S. Re d d y , M. St r i c k e r t , H. A. Gr i m b e r g , S. St y m n e & K. De h e s h : Cofactome analyses reveal Ro l l e t s c h e k , U. Sc h o l z , U. Wo b u s & N. Sreenivasulu : ABA biosynthesis enhanced flux of carbon into oil for potential biofuel and degradation contributing to ABA homeostasis during production. Plant J. 67 (2011) 1018-1028. barley seed development under control and terminal drought- Hö l z l e , A., C. Jo n i e t z , O. Tö r j e k , T. Al t m a n n , S. Bi n d e r & J. Fo r n e r : A stress conditions. J. Exp. Bot. 62 (2011) 2615-32. RESTORER OF FERTILITY-like PPR gene is required for 5’-end Th i e l , J., H. Ro l l e t s c h e k , S. Fr i e d e l , J.E. Lu n n , T.H. Ng u y e n , R. Fe i l , H. processing of the nad4 mRNA in mitochondria of Arabidopsis Ts c h i e r s c h , M. Mü l l e r & L. Bo r i s j u k : Seed-specific elevation of thaliana. Plant J. 65 (2011) 737-744. non-symbiotic hemoglobin AtHb1: beneficial effects and Ja s i k , J., S. Sc h i e b o l d , H. Ro l l e t s c h e k , P. De n o l f , K. v a n Ad e n h o v e , underlying molecular networks in Arabidopsis thaliana. BMC T. Al t m a n n & L. Bo r i s j u k : Subtissue-specific evaluation of Plant Biol. 11 (2011) 48. promoter efficiency by quantitative fluorometric assay in Ts c h i e r s c h , H., L. Bo r i s j u k , T. Ru t t e n & H. Ro l l e t s c h e k : Gradients of laser microdissected tissues of rapeseed. Plant Physiol. 157 seed photosynthesis and its role for oxygen balancing. (2011) 563-573. Biosystems 103 (2011) 302-308. Me l k u s , G., H. Ro l l e t s c h e k , J. Fu c h s , V. Ra d c h u k , E. Gr a f a h r e n d -Be l a u , N. Sreenivasulu , T. Ru t t e n , D. We i e r , N. He i n z e l , F. Sc h r e i b e r , T. Al t m a n n , P.M. Ja k o b & L. Bo r i s j u k : Dynamic (13) C/(1) H NMR Books and Book Chapters imaging uncovers sugar allocation in the living seed. Plant Biotechnol. J. 9 (2011) 1022-1037. 2011 Mu r a y a , M.M., S. d e Vi l l i e r s , H.K. Pa r z i e s , E. Mu t e g i , F. Sa g n a r d , B.M. Al t m a n n , T.: Zugang zu neuen FuE-Konzepten durch innovative Ka n y e n j i , D. Ki a m b i & H.H. Ge i g e r : Genetic structure and diversity Verfahren der Pflanzenphänotypisierung. In: Sc h u m a n n , G., of wild sorghum populations (Sorghum spp.) from different C. St e p h a n & G. Ha r r i n g (Eds.): Erstes Symposium Zierpflan- eco-geographical regions of Kenya. Theor. Appl. Genet. 123 zenzüchtung (15.-16.11.2011, Quedlinburg), Proceedings, (2011) 571-583. Julius-Kühn-Archiv, 433. Arno Brynda GmbH, Berlin (2011) Mu r a y a , M.M., H.H. Ge i g e r , S. d e Vi l l i e r s , F. Sa g n a r d , B.M. Ka n y e n j i , 40-45. D. Ki a m b i & H.K. Pa r z i e s : Investigation of pollen competition Ts c h i e r s c h , H., G. Li e b s c h , A. Stangelmayer , L. Bo r i s j u k & H. Ro l l e t s c h e k : between wild and cultivated sorghums (Sorghum bicolor (L.) Planar oxygen sensors for non invasive imaging in Moench) using simple sequence repeats markers. Euphytica experimental biology. In: Minin, I. (Ed.): Microsensors. InTech 178 (2011) 393-401. (2011) 281-294. Mu r a y a , M.M., E. Mu t e g i , H.H. Ge i g e r , S.M. d e Vi l l i e r s , F. Sa g n a r d , B.M. Ka n y e n j i , D. Ki a m b i & H.K. Pa r z i e s : Wild sorghum from different eco-geographic regions of Kenya display a mixed mating Other Papers system. Theor. Appl. Genet. 122 (2011) 1631-1639. 2010 Mu t e g i , E., F. Sa g n a r d , K. Se m a g n , M. De u , M. Mu r a y a , B. Ka n y e n j i , S. d e Al t m a n n , T.: Plant Genes Matter – integrative plant research for Vi l l i e r s , D. Ki a m b i , L. He r s e l m a n & M. La b u s c h a g n e : Genetic structure future needs. Publ. Serv. Rev.: Sci Technol 8 (2010) 198-199. and relationships within and between cultivated and wild sorghum (Sorghum bicolor (L.) Moench) in Kenya as revealed by microsatellite markers. Theor. Appl. Genet. 122 (2011) 989- PhD and Diploma Thesis 1004. 2010 Ro l l e t s c h e k , H., G. Me l k u s , E. Gr a f a h r e n d -Be l a u , J. Fu c h s , N. He i n z e l , F. We i g e l t , K.: Transkriptionelle und biochemische Untersuchun- Sc h r e i b e r , P.M. Ja k o b & L. Bo r i s j u k : Combined noninvasive imaging gen des veränderten Stickstoff- und Kohlenstoffmetabolis- and modeling approaches reveal metabolic compartmentation mus in genetisch modifizierten Erbsensamen. (PhD Thesis) in the barley endosperm. Plant Cell 23 (2011) 3041-3054. Martin-Luther-Universität Halle-Wittenberg, Institut für Biologie, Halle/S. (2010) 237 pp.

99 Abteilung Molekulare Genetik/Department of Molecular Genetics

Research Group: Seed Development Head: Dr. Winfriede Weschke

Scientists Research Report

IPK financed (1) Metabolic regulation of seed development Meitzel, Tobias (0.50, 01.04.-30.04.2010) In frame of the GABI-SysSEED project, developing grains of Radchuk, Ruslana, Dr. (0.50, till 30.06.2010) the barley endosperm mutant Risø 16 that is deficient in the Sreenivasulu, Nese, Dr. (01.07.-31.10.2010) cytosolic small subunit of ADP-glucose pyrophosphorylase Thiel, Johannes, Dr. (16.02.-30.04.2011) (AGPase) were analysed in detail. On the regulatory level, an Weber, Hans, Dr. overlap of metabolic and hormonal regulation is deduced which leads to parallel transcriptional down-regulation of Grant Positions endosperm-specific genes (storage proteins) and those Andersch, Franka (0.50 DFG, since 01.05.2011) involved in phytohormone (ABA)-induced storage product Harshavardhan, Vokkaliga Thammegowda (0.50 BMBF, accumulation. Results indicate that decreasing carbon fluxes till 31.10.2010) into starch of barley endosperm leads to re-partitioning of Kohl, Stefan (0.50 DFG) carbon, and affects the pathway of amino acid and subsequently, Meitzel, Tobias (0.50 DFG, till 31.03.2010, since 01.05.2010) storage proteins biosynthesis (Faix et al., accepted by Plant J.). Palakolanu, Sudhakar Reddy (0.50 BMBF, 08.03.-30.10.2010) In cooperation with P. Geigenberger (University Munich) starch Radchuk, Ruslana, Dr. (0.50 BMBF, 01.07.2010-28.02.2011) metabolism was analysed in developing wild type and Risø 16 Radchuk, Volodymyr, Dr. (1.00/0.75 BMBF, till 28.02.2011; grains using a non-aqueous fractionation method. The results DFG, since 01.03.2011) are accepted for publication (Thiessen et al., J. Exp. Bot.). In Seiler, Christiane, Dr. (BMBF, till 30.06.2010) transgenic pea embryos, diminishing of trehalose-6 phosphate Sreenivasulu, Nese, Dr. (BMBF, till 30.06.2010) (T6P) levels leads to a wrinkled seed phenotype. Previous Staroske, Nicole (0.50 BMBF, till 30.06.2010) results suggest that starch biosynthesis is likely be influenced Thiel, Johannes, Dr. (DFG, till 15.02.2011; since 01.05.2011) by T6P-mediated activation of AGPase (T. Meitzel, financed Weier, Diana, Dr. (DFG, till 30.04.2010) by DFG). In cooperation with W. Link (University Göttingen) embryonic heterosis was analysed in V. faba seeds. Hybrid Visiting Scientists/Scholars embryos develop enhanced sink strength which is established Mora Ramirez, Maria Isabel (DAAD, since 01.10.2011) during early seed development and probably mediated by Pirko, Yaroslav, Dr. (DFG, since 03.07.2011) auxin effects (Meitzel et al. 2011). Prasad, Manoj, Dr. (DLR, 12.02.-28.02.2010) Seiler, Christiane, Dr. (self-financed, 01.07.-31.10.2010) (2) Hormonal influences on seed development Staroske, Nicole (self-financed, 01.04.-30.04.2011; In developing pea embryos and developing barley caryopses 01.08.-31.12.2011) abscisic acid (ABA) deficiency was induced by immunomodu- Strickert, Marc, Dr. (self-financed, 01.02.-31.10.2010) lation (cooperation with Udo Conrad). In pea, immunomodu- Tran Thi Thuy, Van (Ministry of Education and Training, lation attenuates developmental phase transition and storage Vietnam, since 21.08.2011) metabolism (Radchuk et al. 2010). In barley, drastically elevated ABA amounts are related to altered metabolite and transcript levels preferentially during the transition phase of endosperm development. Evaluation of transcript profiling data hints to Goals mechanisms reducing ABA sensitivity of transcriptional regulation. As a result, mature transgenic grains show only slight The general goal of our scientific work is to understand and phenotypic variations (N. Staroske, PhD thesis, financed by influence regulatory networks of differentiation and storage BMBF). In the barley mutant seg8, deregulation of ABA con- product accumulation in developing crop seeds. In more tent causes abnormal development of ETCs. Feedback respons- detail, our interest is focused on metabolic and hormonal es between ABA-producing maternal and filial grain tissues regulation of these processes. Thereby, specific emphasis is during the transition phase are suggested (Sreenivasulu et al. laid on differentiation of barley endosperm transfer cells (ETCs). 2010). 454-transcriptome sequencing of microdissected devel- Scientific work is further focused on sink-source relationships oping ETC favours the two component system (TCS) as a major and on cellular disintegration processes in developing seeds. signalling cascade (J. Thiel, in preparation, financed by DFG). Aim of our work is to improve yield and quality of crop seeds. Hormonal influences on the activity of TCS-associated histidine kinases are supposed but not confirmed yet.T ranscript and metabolite profiling of microdissected developing ETCs will probably gain additional insights into hormon signalling

100 Fig. 36 Structural analysis of the cell architecture of the endosperm transfer cells in developing grains of Hordeum vulgare. Light microscopy (a, c, f, i, l) scanning electron microscopy (b, d, g, j, m) and transmission electron microscopy (e, h, k, n) of grains 5 DAF (a, b, c, d, e), 7 DAF (f - h), 10 DAF (i - k) and 12 DAF (l - n). CW, cell wall; Cyt, cytoplasm; DAF, days after flowering; ETC, endosperm transfer cells; M, mitochondria; NP, nucellar projection; P, pericarp; SE, starchy endosperm (J. Thiel, T. Rutten, H. Weber and M. Melzer).

but also regulatory networks responsible for the establishment by DAAD). Transcript and metabolite profiling of remobilising of a specific wall architecture (Fig. 36) typically found in transfer flag leaves, glumes and developing grains suggest that the cells (J. Thiel, in preparation). transient phase of grain development is registered also in ear-near vegetative tissues. A development-specific signal is qRT-PCR analyses of ABA-biosynthesis and signalling genes in supposed to mediate sink-source communication during microdissected tissues of developing barley caryopses suggest grain development (S. Kohl, manuscript in preparation, that ABA synthesised in the maternal chlorenchyma might financed by DFG). be responsible for the activity of ABA-signalling genes in the endosperm (J. Schmeichel, Diploma thesis, IPK-financed). (4) Cellular disintegration and crop seed development In barley, development of maternal grain tissues is mediated by (3) Sink-source relationships regulated cell expansion and cell disintegration and coordinated Sucrose uptake capacity increased by expression of a barley with endosperm growth. Cell disintegration is mediated by sucrose transporter in developing wheat grains (transgenic programmed cell death (PCD) events that might be coupled to model HOSUT) stimulates storage protein synthesis and results the activity of vacuolar processing enzymes (VPEs) (Radchuk in significant increase of thousand grain weight (Weichert et et al. 2011). RNAi suppression of members of the VPE gene family al. 2010). In cooperation with M. Röder, genetic and metabolic will probably show influence of VPE gene activity on barley grain control of grain shape and weight is analysed in HOSUT grains development (V. Tran Thie Thyu). Cellular disintegration and PCD and grains of a wheat introgression line (F. Andersch, financed in the nucellar projection of developing grains are coupled to the by DFG). The HOSUT modulation has also a positive impact on activity of the small cystein-rich protein Jekyll (Radchuk et al. 2006). vegetative development (I. Saalbach, I. Mora Ramirez, financed Jekyll activity has to undergo fine-tuned regulation.T ranscriptional

101 Abteilung Molekulare Genetik/Department of Molecular Genetics

regulation of Jekyll expression and protein composition of the Sh a r m a , S., N. Sreenivasulu , V.T. Harshavardhan , C. Se i l e r , S. Sh a r m a , regulatory Jekyll complex will be analysed in cooperation with Z.N. Kh a l i l , E. Ak h u n o v , S.K. Se h g a l & M.S. Rö d e r : Delineating U. Conrad (V. Radchuk, financed by DFG). Two members of a the structural, functional and evolutionary relationships of gene family encoding small cystein-rich proteins were identified sucrose phosphate synthase gene family II in wheat and in pea (T. Meitzel, R. Radchuk). The respective proteins might be related grasses. BMC Plant Biol. 10 (2010) 134. responsible for cellular disintegration and nutrient release in the Sreenivasulu , N., L. Bo r i s j u k , B.H. Ju n k e r , H.P. Mo c k , H. Ro l l e t s c h e k , U. pea seed coat. Further work will verify this hypothesis. Se i f f e r t , W. We s c h k e & U. Wo b u s : Barley grain development toward an integrative view. Int. Rev. Cell Mol. Biol. 281 (2010) 49-89. Sreenivasulu , N., V. Ra d c h u k , A. Al a w a d y , L. Bo r i s j u k , D. We i e r , N. St a r o s k e , J. Fu c h s , O. Mi e r s c h , M. St r i c k e r t , B. Us a d e l , U. Wo b u s , Publications B. Gr i m m , H. We b e r & W. We s c h k e : De-regulation of abscisic acid contents causes abnormal endosperm development in the Peer Reviewed Papers barley mutant seg8. Plant J. 64 (2010) 589-603. We i c h e r t , N., I. Sa a l b a c h , H. We i c h e r t , S. Ko h l , A. Er b a n , J. Ko p k a , B. 2010 Ha u s e , A. Va r s h n e y , N. Sreenivasulu , M. St r i c k e r t , J. Ku m l e h n , W. Ka s p a r , S., D. We i e r , W. We s c h k e , H.P. Mo c k & A. Ma t r o s : Protein We s c h k e & H. We b e r : Increasing sucrose uptake capacity of analysis of laser capture micro-dissected tissues revealed wheat grains stimulates storage protein synthesis. Plant cell-type specific biological functions in developing barley Physiol. 152 (2010) 698-710. grains. Anal. Bioanal. Chem. 398 (2010) 2883-2893. Lo h s e , M., A. Nu n e s -Ne s i , P. Kr ü g e r , A. Na g e l , J. Ha n n e m a n n , F.M. Gi o r g i , 2011 L. Ch i l d s , S. Os o r i o , D. Wa l t h e r , J. Se l b i g , N. Sreenivasulu , M. St i t t , An d r i u n a s , F.A., H.M. Zh a n g , H. We b e r , D.W. McCu r d y , C.E. Of f l e r A.R. Fe r n i e & B. Us a d e l : Robin: an intuitive wizard application & J.W. Pa t r i c k : Glucose and ethylene signalling pathways for R-based expression microarray quality assessment and converge to regulate trans-differentiation of epidermal analysis. Plant Physiol. 153 (2010) 642-651. transfer cells in Vicia narbonensis cotyledons. Plant J. 68 Ra d c h u k , R., U. Co n r a d , I. Sa a l b a c h , M. Gi e r s b e r g , R.J. Em e r y , H. Kü s t e r , (2011) 987-998. A. Nu n e s -Ne s i , A.R. Fe r n i e , W. We s c h k e & H. We b e r : Abscisic Go v i n d , G., C. Se i l e r , U. Wo b u s & N. Sreenivasulu : Importance of ABA acid deficiency of developing pea embryos achieved by homeostasis under terminal drought stress in regulating immunomodulation attenuates developmental phase grain filling events. Plant Signal. Behav. 6 (2011) 1228-1231. transition and storage metabolism. Plant J. 64 (2010) 715- Me i t z e l , T., R. Ra d c h u k , A. Nu n e s -Ne s i , A.R. Fe r n i e , W. Li n k , W. We s c h k e 730. & H. We b e r : Hybrid embryos of Vicia faba develop enhanced Ra d c h u k , R., N.E. Em e r y , D. We i e r , H. Vi g e o l a s , P. Geigenberger , J.E. Lu n n , sink strength, which is established during early development. R. Fe i l , W. We s c h k e & H. We b e r : Sucrose non-fermenting kinase Plant J. 65 (2011) 517-531. 1 (SnRK1) coordinates metabolic and hormonal signals Me l k u s , G., H. Ro l l e t s c h e k , J. Fu c h s , V. Ra d c h u k , E. Gr a f a h r e n d -Be l a u , during pea cotyledon growth and differentiation. Plant J. 61 N. Sreenivasulu , T. Ru t t e n , D. We i e r , N. He i n z e l , F. Sc h r e i b e r , T. (2010) 324-338. Al t m a n n , P.M. Ja k o b & L. Bo r i s j u k : Dynamic (13) C/(1) H NMR Ri e b e s e e l , E., R.E. Hä u s l e r , R. Ra d c h u k , T. Me i t z e l , M.R. Ha j i r e z a e i , R.J. imaging uncovers sugar allocation in the living seed. Plant Ne i l Em e r y , H. Kü s t e r , A. Nu n e s -Ne s i , A.R. Fe r n i e , W. We s c h k e & H. Biotechnol. J. 9 (2011) 1022-1037. We b e r : The 2-oxoglutarate/malate translocator mediates Ra d c h u k , V., D. We i e r , R. Ra d c h u k , W. We s c h k e & H. We b e r : amino acid and storage protein biosynthesis in pea embryos. Development of maternal seed tissue in barley is mediated Plant J. 61 (2010) 350-363. by regulated cell expansion and cell disintegration and coordinated with endosperm growth. J. Exp. Bot. 62 (2011) 1217-1227.

102 Se i l e r , C., V.T. Harshavardhan , K. Ra j e s h , P.S. Re d d y , M. St r i c k e r t , H. 2011 Ro l l e t s c h e k , U. Sc h o l z , U. Wo b u s & N. Sreenivasulu : ABA biosynthesis Th i e l , J., D. We i e r & W. We s c h k e : Laser capture microdissection of and degradation contributing to ABA homeostasis during developing barley seeds and cDNA array analysis of selected barley seed development under control and terminal drought- tissues. In: Mu r r a y , G.I. (Ed.): Laser capture microdissection: stress conditions. J. Exp. Bot. 62 (2011) 2615-32. methods and applications, 2nd Edition (Methods Mol. Biol. Th i e l , J., H. Ro l l e t s c h e k , S. Fr i e d e l , J.E. Lu n n , T.H. Ng u y e n , R. Fe i l , H. 755). Springer New York (2011) 461-475. Ts c h i e r s c h , M. Mü l l e r & L. Bo r i s j u k : Seed-specific elevation of non-symbiotic hemoglobin AtHb1: beneficial effects and underlying molecular networks in Arabidopsis thaliana. BMC Other Papers Plant Biol. 11 (2011) 48. 2010 Wi t z e l , K., C. Pi e t s c h , M. St r i c k e r t , A. Ma t r o s , M. Rö d e r , W. We s c h k e , Sreenivasulu , N., M.S. Rö d e r & U. Wo b u s : Trockenstress – eine Suche U. Wo b u s & H.P. Mo c k : Mapping of quantitative trait loci nach den Ursachen und nach neuen Wegen zur Züchtung associated with protein expression variation in barley grains. trockentoleranter Getreide. GenomXPress 4 (2010) 4-6. Mol. Breed. 27 (2011) 301-314. Wo r c h , S., K. Ra j e s h , V.T. Harshavardhan , C. Pi e t s c h , V. Ko r z u n , L. Ku n t z e , A. Bö r n e r , U. Wo b u s , M.S. Rö d e r & N. Sreenivasulu : Haplotyping, PhD and Diploma Theses linkage mapping and expression analysis of barley genes 2010 regulated by terminal drought stress influencing seed An d e r s c h , F.: Seneszenz-assoziierter Stickstofftransport in Gerste. quality. BMC Plant Biol. 11 (2011) 1. (Diploma Thesis) Friedrich-Schiller-Universität, Jena (2010) 104 pp. We i g e l t , K.: Transkriptionelle und biochemische Untersuchungen Books and Book Chapters des veränderten Stickstoff- undK ohlenstoffmetabolismus in genetisch modifizierten Erbsensamen. (PhD Thesis) Martin- 2010 Luther-Universität Halle-Wittenberg, Institut für Biologie, Sreenivasulu , N., R. Su n k a r , U. Wo b u s & M. St r i c k e r t : Array platforms Halle/S. (2010) 237 pp. and bioinformatics tools for the analysis of plant transcriptome in response to abiotic stress. In: Su n k a r , R. (Ed.): Plant 2011 Stress Tolerance – Methods and Protocols, Methods Mol. Sc h m e i c h e l , J.: Establishment of a development and tissue spe- Biol., Vol. 639. Humana Press (2010) 71-93. cific cDNA collection to monitor gene expression gradients We b e r , H., N. Sreenivasulu & W. We s c h k e : Molecular physiology of of ABA biosynthesis enzymes in developing barley grains. seed maturation and seed storage protein biosynthesis. In: (Diploma Thesis) Friedrich-Schiller-Universität, Jena (2011) Pu a , E.-C. & M.R. Da v e y (Eds.): Plant Developmental Biology – 62 pp. Biotechnological Perspectives, Vol. 2. Springer, Berlin (2010) St e f a n i , D.: Protein-Protein-Interaktionsstudien an pflanzlichen 83-104. Trehalose-6-Phosphatsynthasen und -Phosphatasen. (Di- Wo b u s , A.M., U. Wo b u s & B. Pa r t h i e r (Eds.): Der Begriff der Natur ploma Thesis) Martin-Luther-Universität Halle-Wittenberg, – Wandlungen unseres Naturverständnisses und seine Fol- Halle/S. (2011) 88 pp. gen. Gaterslebener Begegnung 2009. Nova Acta Leopoldi- na, Bd. 109, Heft 376, Wissenschaftliche Verlagsgesellschaft, Stuttgart (2010) 266 pp. Wo b u s , A.M. & U. Wo b u s : Gaterslebener Begegnung 2009. Der Be- griff der Natur – Wandlungen unseres Naturverständnisses und seine Folgen. In: Jahrbuch 2009 der Deutschen Aka- demie der Naturforscher Leopoldina (Halle/S.) LEOPOLDINA (R.3), 55 (2010) 419-420.

103 Abteilung Molekulare Genetik/Department of Molecular Genetics

Research Group: Gene Regulation Head: Dr. Helmut Bäumlein

Scientists Another approach concerns the identification of regulatory networks that control gametophytic development. Based IPK financed on egg cell-specific genes of wheatA ( . Czihal), we discovered Kirioukhova, Olga, Dr. (since 01.07.2011) a novel class of transcription factors in Arabidopsis, designated Köszegi, David, Dr. (till 30.06.2011) as RKD factors (D. Köszegi, A.J. Johnston, O. Kirioukhova). The Lee Hong, Diep (0.50, 01.04.-30.09.2010) plant-specific occurrence, egg cell-specific expression, the mis- Mildner, Maria (0.50 Pakt für Forschung und Innovation, expression phenotype and evolutionary conservation identify till 28.02.2010; 0.50, 01.03.-31.03.2010) RKD factors as key regulators of female gamete identity (see Fig. 37). Ectopic expression of RKD genes causes the reprogramming Grant Positions of sporophytic cells to adopt aspects of egg cell identity (Köszegi Gryczka, Corina, Dr. (BMBF, till 31.08.2010) et al. 2011). Currently, we aim to identify promoter regions Johnston, Amal Joseph, Dr. (DFG, till 31.08.2010) responsible for egg cell-specific expression A( . Czihal, M. Mildner, Kirioukhova, Olga, Dr. (DFG, 01.09.2010-30.06.2011) D. Köszegi) and to isolate multiple mutants in RKD genes, also Köszegi, David, Dr. (0.50 DFG, since 01.07.2011) including the TALEN technology (O. Kirioukhova, D. Köszegi, A.J. Rizzo, Paride (0.50 DFG, since 01.07.2011) Johnston; cooperation with J. Boch, Halle/S.). Schallau, Anna, Dr. (0.75/1.00 DFG, since 01.03.2011)

Visiting Scientists/Scholars Johnston, Amal Joseph, Dr. (Humboldt fellowship, since 01.09.2010) Lee Hong, Diep (Ministry of Education and Training Vietnam, till 31.03.2010; 01.10.2010-14.03.2011) Mildner, Maria (self-financed, 01.10.-31.10.2010) Sarahnova, Petra (DAAD, 28.10.2010-07.02.2011; since 04.10.2011) Schallau, Anna, Dr. (self-financed/IPK, 11.08.2010-28.02.2011)

Goals

Analysis of gene networks during gametophyte development and embryogenesis.

Fig. 37 Research Report Egg cell-specific promoter activity of a RKD-controlled gene (D. Köszegi).

The group deals with the molecular dissection of genetic We concluded work on the regulation of gene expression dur- and epigenetic pathways that control sexual and asexual ing late embryogenesis. ChIP-chip experiments and tran- plant reproduction including zygotic, apomictic, somatic and scriptome analysis has been applied to identify putative target androgenetic processes. genes of the transcription factors LEC1 and ABI3 (A. Junker, collaboration with research group Phytoantibodies and research In Hypericum, we have identified and characterised HAPPY, group Expression Mapping). These approaches suggest novel a locus which co-segregates with apospory, an important phytohormone-mediated functions of LEC1 during embryo- component of apomixis (A. Schallau, A.J. Johnston, D. Köszegi; genesis and skotomorphogenic growth and identify 98 target cooperation with G. Barcaccia, Italy). Apospory is inherited as genes of ABI3, representing the ABI3 regulon. a dominant simplex locus at the tetraploid level. An apospory- specific CAPS marker has been applied to isolate both sexual (150 kb) and aposporous alleles (400 kb) of the HAPPY locus. The apo-linked allele contains destroyed genes, is extended by the insertion of copia-like transposons and composed of three contributing sexual loci (collaboration with research groups Expression Mapping, Apomixis and Experimental Taxonomy).

104 In an attempt to understand the molecular reprogramming Ju n k e r , A., A. Ha r t m a n n , F. Sc h r e i b e r & H. Bä u m l e i n : An engineer’s events during androgenesis as basis for the application of view on regulation of seed development. Trends Plant Sci. double haploid technology in crop plants, we have analysed 15 (2010) 303-307. differential gene expression during pollen embryogenesis. Sc h a l l a u , A., F. Ar z e n t o n , A.J. Jo h n s t o n , U. Hä h n e l , D. Kő s z e g i , F. Deep sequencing of the transcriptome of isolated microspores Bl a t t n e r , L. Al t s c h m i e d , G. Ha b e r e r , G. Ba r c a c c i a & H. Bä u m l e i n : resulted in the identification of candidate genes likely to be Identification and genetic analysis of the APOSPORY locus involved in the control of this process (C. Gryzcka, D. Köszegi, in Hypericum perforatum L. Plant J. 62 (2010) 773-784. A. Czihal, collaboration with research group Plant Reproductive Biology). 2011 Ga l l a , G., G. Ba r c a c c i a , A. Sc h a l l a u , M. Pu e n t e Mo l i n s , H. Bä u m l e i n We have further contributed to the functional characterisation of a & T.F. Sh a r b e l : The cytohistological basis of apospory in novel family of transcription factors, EFFECTOR OF TRANSCRIPTION Hypericum perforatum L. Sex. Plant Reprod. 24 (2011) 47-61. (ET). ET factors function as regulators of other transcription He c k m a n n , S., I. Le r m o n t o v a , B. Be r c k m a n s , L. De Ve y l d e r , H. Bä u m l e i n factors and participate in the control of cell differentiation, & I. Sc h u b e r t : The E2F transcription factor family regulates including processes during plant reproduction (L.H. Diep, A.J. CENH3 expression in Arabidopsis thaliana. Plant J. 68 (2011) Johnston). 646-656. Kirioukhova, O., A.J. Jo h n s t o n , D. Kl e e n , C. Kä g i , R. Ba s k a r , J.M. Further work, coordinated by A. J. Johnston, concerns the char- Mo o r e , H. Bä u m l e i n , R. Gr o s s -Ha r d t & U. Grossniklaus : Female acterisation of a plant homologue of the mammalian tumour gametophytic cell specification and seed development and cell cycle suppressor RETINOBLASTOMA (pRB) involved in require the function of the putative Arabidopsis INCENP the control of cell fate of gametes. RBR is a key component of ortholog WYRD. Development 138 (2011) 3409-3420. regulatory network controlling DNA and histone methylation Kő s z e g i , D., A.J. Jo h n s t o n , T. Ru t t e n , A. Cz i h a l , L. Al t s c h m i e d , J. during gametic development. Based on a newly isolated, ge- Ku m l e h n , S.E. Wu s t , O. Kirioukhova, J. Gh e y s e l i n c k , U. Grossniklaus netically characterised hypomorphic homozygous rbr mutant & H. Bä u m l e i n : Members of the RKD transcription factor family we investigate a novel role for RBR pathway during early germ- induce an egg cell-like gene expression program. Plant J. 67 line development. Preliminary data suggest that acute reduc- (2011) 280-291. tion of RBR leads to the initiation of events similar “apomeio- Le r m o n t o v a , I., O. Ko r o l e v a , T. Ru t t e n , J. Fu c h s , V. Sc h u b e r t , I. Mo r a e s , sis” (R. Lemcke, O. Kiroukhova, D. Köszegi; collaboration with D. Kö s z e g i & I. Sc h u b e r t : Knockdown of CENH3 in Arabidopsis W. Gruissem, Zürich). We have also identified putative target reduces mitotic divisions and causes sterility by disturbed genes of RBR that are derepressed in this mutant as part of a meiotic chromosome segregation. Plant J. 68 (2011) 40-50. novel regulatory circuit involving DNA methylation. Finally, we aim to understand the epigenetic control of apomixis in various Boechera species. We have cloned several epigenetic loci PhD and Diploma Theses that show heterochronic gene expression in apomicitic and sexual accessions (P. Rizzo, collaboration with research group 2010 Plant Reproductive Biology). The data are anticipated to extend Sc h a l l a u , A.: Identification and genetic analysis of the APOS- our knowledge on sexual and apomictic reproductive develop- PORY locus in Hypericum perforatum L. (PhD Thesis) Martin- ment. Luther-Universität Halle-Wittenberg, Naturwissenschaftli- che Fakultät I, Institut für Genetik, Halle/S. (2010) 171 pp.

2011 Publications Le, H.D.: Functional characterization of Effector ofT ranscription (ET) in Arabidopsis thaliana. (PhD Thesis) Martin-Luther-Uni- Peer Reviewed Papers versität Halle-Wittenberg, Naturwissenschaftliche Fakultät I, Institut für Biologie, Halle/S. (2011) 127 pp. 2010 Le m c k e , R.: The RETINOBLASTOMA pathway controls reproduc- Aq u e a , F., A.J. Jo h n s t o n , P. Ca n o n , U. Grossniklaus & P. Ar c e -Jo h n s o n : tive development and genome integrity in Arabidopsis. TRAUCO, a Trithorax-group gene homologue, is required for (Bachelor) Martin-Luther-Universität Halle-Wittenberg, early embryogenesis in Arabidopsis thaliana. J. Exp. Bot. 61 Halle/S. (2011) 43 pp. (2010) 1215-1224. Mi l d n e r , M.: Funktionelle Charakterisierung von Eizell-aktiven Jo h n s t o n , A.J., O. Kirioukhova, P.J. Ba r r e l l , T. Ru t t e n , J.M. Mo o r e , RKD-Faktoren in Arabidopsis thaliana. (PhD Thesis) Martin- R. Ba s k a r , U. Grossniklaus & W. Gr u i s s e m : Dosage-sensitive Luther-Universität Halle-Wittenberg, Halle/S. (2011) 128 pp. function of RETINOBLASTOMA RELATED and convergent epigenetic control are required during the Arabidopsis life cycle. PLoS Genet. 6 (2010) e1000988.

105 Abteilung Molekulare Genetik/Department of Molecular Genetics

Research Group: Phytoantibodies Head: Dr. Udo Conrad

Scientists but not in water (N. Weichert, V. Hauptmann, M. Gils, U. Conrad). Raster scanning electron microscopic pictures of this material IPK financed show extended nanofibers (M. Menzel, U. Spohn, Fraunhofer IWM Mönke, Gudrun, Dr. Halle).

Grant Positions Different antigens from the bird flue virusH 5N1 (hemagglutinin, Weichert, Nicola, Dr. (BMBF, since 01.01.2011) neuraminidase, matrix protein) with and without ELP have Hauptmann, Valeska (0.50 Saxony-Anhalt, till 28.02.2011) been stably expressed in tobacco leaves and seeds. An mITC technology for plant-expressed ELPylated proteins has been Visiting Scientists/Scholars developed and applied for hemagglutinin and neuraminidase. Herrmann, Isabella (Martin Luther University Halle-Wittenberg, The quality of these antigens (no proteolysis, high activity in the till 30.06.2010) case of neuraminidase) has been highly improved. 95 % of the Hoang, Phan Trong (DLR) antigens in the plant crude extract could be recovered using this Huu Cuong, Nguyen, Dr. (DAAD, 08.08.-04.10.2010) improved purification method. Fusion of a Leucin zipper-based Sarriòn Perdigones, Alejandro (Instituto de Biologia Molecular trimerisation motif to the C-terminus of hemagglutinin caused y Celular de Plantas Valencia, Spain, (17.09.-19.11.2011) the expression of hemagglutinin trimers in tobacco plants. The expression of full-length hemagglutinin (inclusive membrane domain and cytoplasmic tail) using a secretion vector (signal Goals peptide, no KDEL) allowed the formation and isolation of virus- like-particles (VLPs) from tobacco plants. These particles as Production of recombinant fiber proteins, recombinant vac- well as inactivated viruses and hemagglutinin trimers were cines, recombinant therapeutic antibodies in transgenic plants, active in a standard hemagglutination assay. Immunisation tissue- and development-specific immunomodulation of studies with mice showed, that only by immunisation with phytohormone functions in transgenic plants and molecular trimers of hemagglutinin antibodies inhibiting the activity analysis of seed development by chromatin IP method for the of VLPs in the hemagglutination assay (potentially neutralising molecular analysis of seed development. antibodies) could be raised (P.T. Hoang, U. Conrad, J. Veits, FLI Riems, F. Rabenstein, JKI Quedlinburg). Different hemagglutinin variants (fused to ELP and to hydrophobin) have been expressed Research Report in tobacco seeds and are currently investigated according subcellular localisation (P.T. Hoang, E. Arcalis, BOKU Vienna). Molecular Farming experiments were performed with Re-screening of synthetic Phage Display Libraries based on recombinant spider silk proteins to develop new materials for cameloid VHH led to the isolation of new specific nanobodies technical and medical purposes in plants. A set of dragline silk against human TNFalpha with binding behavior and TNFalpha- protein (MaSpI, MaSpII) and flagelliform silk (FLAG) protein neutralising capacity comparable to the parental antibody expression vectors containing 2 to 4 spider silk elements and (library basis), but with either completely changed CDR1 or CDR3 1 ELP element has been designed and transiently expressed in sequence (U. Conrad, J. Scheller, University Düsseldorf). tobacco (V. Hauptmann). We further developed the concept of multimeric spider silk proteins by the transglutaminase-based To understand the role of ABI3, a seed-specific transcription fac- dimerisation. Here the purification of the monomers and of the tor, we in more detail identifiedgenome-wide target genes of dimers has been essentially improved by the development and this transcription factor, employing ChIP-chip technology, tran- adaptation of a membrane-based Inverse Transition Cycling scriptome analysis, qRT-PCR and a transient promoter activa- procedure (N. Weichert, P.T. Hoang). Flagelliform spider silk-like tion assay. 98 genes were verified by at least two methods to be protein expression vectors have been designed, that allow intein- activated by ABI3. Most of the genes fit to functions in seed de- based trans-splicing. Native-sized flagelliform silk multimers up to velopment based on gene ontology terms. Several gene func- a molecular weight of 500 kDa have been produced in transiently tions are still unknown. The related transcription factor FUS3 and stably transformed tobacco leaves. Mutations in the relevant activates more than one third of the ABI3 target genes, amino acid coding sequences in the intein region resulted in the proofed by qRT-PCR. Within the 98 promoters of the ABI3 tar- expression of non-spliced monomers after transformation into get genes G-box (CACGTG) – like and/or RY (CATGCA)-like mo- tobacco, thus showing that the multimerisation is due to intein tifs were discovered using the Dispom algorithm (G. Mönke, J. function. After affinity purification, desalting and concentration Keilwagen, L. Altschmied, H. Bäumlein). by ultrafiltration with high size exclusion (150 kDa) the FLAG protein multimers were lyophilised and could be dissolved in HFIP

106 In vitro binding studies of ABI3 and FUS3 to variants of the RY Sa u m o n n e a u , A., K. Ro t t i e r , U. Co n r a d , Y. Po p i n e a u , M. Fr a n c i n -Al l a m i motif showed that one exchange of the first five nucleotides & J. Gu e g u e n : Expression of a new chimeric protein with a prevents binding completely. Only the last A can be partially highly repeated sequence in tobacco cells. Plant Cell Rep. 30 replaced by G. In vivo activation studies are going on (G. Mönke, (2011) 1289-1302. U. Conrad). The experiments according immunomodulation of ABA functions in barley and pea are reported by the Seed Books and Book Chapters Development group. 2010 Co n r a d , U. & D.M. Fl o s s : Expression of antibody fragments in transgenic plants. In: Ko n t e r m a n n , R. & S. Dü b e l (Eds.): Publications Antibody Engineering, Vol. 2 (2nd edition). Springer-Verlag, Berlin-Heidelberg (2010) 377-386. Peer Reviewed Papers Fl o s s , D.M. & U. Co n r a d : Expression of complete antibodies in transgenic plants. In: Ko n t e r m a n n , R. & S. Dü b e l (Eds.): 2010 Antibody Engineering, Vol. 1 (2nd edition). Springer-Verlag, Fl o s s , D.M., M. Mo c k e y , G. Za n e l l o , D. Br o s s o n , M. Di o g o n , R. Fr u t o s , Berlin-Heidelberg (2010) 489-502. T. Br u e l , V. Ro d r i g u e s , E. Ga r z o n , C. Ch e v a l e y r e , M. Be r r i , H. Sa l m o n , U. Co n r a d & L. De d i e u : Expression and immunogenicity of the mycobacterial Ag85B/ESAT-6 antigens produced in PhD and Diploma Theses transgenic plants by Elastin-like peptide fusion strategy. J. 2010 Biomed. Biotechnol. (2010) 274346. Ga s t a l d e l l o , J.: Funktionelle Charakterisierung neuer anti hTNFα Fl o s s , D.M., K. Sc h a l l a u , S. Ro s e -Jo h n , U. Co n r a d & J. Sc h e l l e r : VHH cameloid nanobodies. (Bachelor Thesis) Hochschule Elastin-like polypeptides revolutionise recombinant Anhalt, Köthen (2010) 84 pp. protein expression and their biomedical application. Trends Ku n z e , M.: Charakterisierung des Abscisinsäure-Rezeptor bin- Biotechnol. 28 (2010) 37-45. denden Antikörpers anti-RCAR1. (Bachelor Thesis) Martin- Gi e r s b e r g , M., D.M. Fl o s s , S. Kipriyanov, U. Co n r a d & J. Sc h e l l e r : Luther-Universität Halle-Wittenberg, Naturwissenschaftli- Covalent dimerization of camelidae anti-human TNF-alpha che Fakultät I, Institut für Biochemie und Biotechnologie, single domain antibodies by the constant kappa light chain Halle/S. (2010) 50 pp. domain improves neutralizing activity. Biotechnol. Bioeng. 106 (2010) 161-166. 2011 Ra d c h u k , R., U. Co n r a d , I. Sa a l b a c h , M. Gi e r s b e r g , R.J. Em e r y , H. Ba n i k , A.: Funktionelle Charakterisierung von rekombinanten Anti- Kü s t e r , A. Nu n e s -Ne s i , A.R. Fe r n i e , W. We s c h k e & H. We b e r : Abscisic körpern gegen Abszisinsäurerezeptoren. (Bachelor Thesis) Mar- acid deficiency of developing pea embryos achieved by tin-Luther-Universität Halle-Wittenberg, Halle/S. (2011) 55 pp. immunomodulation attenuates developmental phase Kn o c h , D.: Expression, Reinigung und Charakterisierung von transition and storage metabolism. Plant J. 64 (2010) 715- Spinnenseidenproteinmultimeren aus Tabak. (Bachelor 730. Thesis) Martin-Luther-Universität Halle-Wittenberg, Halle/S. Vo g e l , M., M. La w s o n , W. Si p p l , U. Co n r a d & W. Ro o s : Sanguinarine (2011) 65 pp. reductase – structure and mechanism of an enzyme of Po h l , J.: Produktion von oligomeren Vogelgrippeantigenen als alkaloid detoxication. J. Biol. Chem. 285 (2010) 18397- ELP-Fusionsproteine in Pflanzen. (Bachelor Thesis) Hoch- 18406. schule Anhalt, Köthen (2011) 67 pp. Ro t h e r , E.: Herstellung und Charakterisierung von scFv´s gegen 2011 den Phytohormonrezeptor RCAR1. (Bachelor Thesis) Hoch- Co n r a d , U., I. Pl a g m a n n , S. Ma l c h o w , M. Sa c k , D.M. Fl o s s , A.A. schule Mittweida, Mittweida (2011) 69 pp. Kr u g l o v , S.A. Ne d o s p a s o v , S. Ro s e -Jo h n & J. Sc h e l l e r : ELPylated Zi e r m a n n , H.: Untersuchungen zur Sequenzspezifität derB indung anti-human TNF therapeutic single-domain antibodies von Transkriptionsfaktoren an DNA. (Bachelor Thesis) Martin- for prevention of lethal septic shock. Plant Biotechnol. J. 9 Luther-Universität Halle-Wittenberg, Halle/S. (2011) 48 pp. (2011) 22–31. Mi e l k e , K., S. Fo r n e r , R. Kr a m e l l , U. Co n r a d & B. Ha u s e : Cell-specific visualization of jasmonates in wounded tomato and Patents Arabidopsis leaves using jasmonate-specific antibodies.N ew 2010 Phytol. 190 (2011) 1069-1080. Co n r a d , U. & M. Gi e r s b e r g : „Fusionsantikörper“. WO 2010/079149, Ph a n , H.T. & U. Co n r a d : Membrane-based inverse transition Anmeldetag: 09.01.2009, Offenlegung: 15.07.2010, IPK Nr. cycling: an improved means for purifying plant-derived 2008/10. recombinant protein-elastin-like polypeptide fusions. Int. J. Co n r a d , U., M. Gi e r s b e r g , S. Kiprijanov & S. Hä h n e l : “Proteolytically Mol. Sci. 12 (2011) 2808-2821. stable antibody formats”. WO 2010/040545, Anmeldetag: 06.10.2008, Offenlegung: 15.04.2010, IPK Nr. 2008/09.

107 Abteilung Molekulare Genetik/Department of Molecular Genetics

Research Group: Expression Mapping (till 31 July 2011) Head: Dr. Lothar Altschmied

Scientists Annotation of the 400 kbp HAPPY locus sequence revealed a complex pattern of repeats involving pieces of genes of IPK financed the three contributing sexual loci separated by long regions Witkowicz, Justyna, Dr. (till 30.06.2010) containing mostly transposons. Pieces of the NPH3 gene for instance occur three times, two times in tandem and once in Visiting Scientists/Scholars an inverted orientation. Despite this complicated structure and Hähnel, Urs, Dr. (Julius Kühn-Institute Quedlinburg, the repetitive nature of most of the sequences within the locus, till 16.05.2010) a chromosome walking strategy was applied, to extend BAC coverage of the HAPPY locus. Seven BAC clones on one side and three clones on the other side of the contig were identified (D. Goals Fiedler, L. Altschmied) using hybridisation and PCR strategies to distinguish the sexual and various apomictic alleles. The longest Characterisation of the physical structure of the apomeiosis and least overlapping clone was chosen on either side of the locus of Hypericum perforatum. Analysis of promoter structure contig and sequenced (A. Himmelbach, Genome Diversity and S. and transcription factors responsible for seed-specific gene König, Transcriptome Analysis). expression in Arabidopsis thaliana.

Research Report

BAC clones containing the apomictic allele of the apomeiosis locus of Hypericum perforatum (HAPPY) had been isolated by A. Schallau and A. Czihal (Gene Regulation group), sequenced (A. Himmelbach, Genome Diversity group and S. König, Transcriptome Analysis group), and assembled (B. Steuernagel, Bioinformatics and Information Technology group). Annotation of these clones (L. Altschmied), which constitute at least 400 kbp of the apomeiosis locus, revealed, that besides pieces of genes known from the sexual allele of this locus (from plants with sexual mode of reproduction), pieces of additional genes are present within the locus. Judged on the available sequence information, none of the gene pieces in the apomeiosis locus represents a functional gene, because reading frames are interrupted by stop codons, frame shifts and transposon insertions. Hybridisation of probes derived from these additional gene pieces with high-density colony filters (D. Fiedler) of Hypericum BAC libraries from sexual and apomictic plants demonstrated that the additional gene pieces originate from two sexual loci. BAC clones containing these two loci were isolated from plants reproducing sexually (I. Dommes, D. Fiedler), sequenced (A. Himmelbach, Genome Diversity group and S. König, Transcriptome Analysis group) assembled (B. Steuernagel, Bioinformatics and Information Technology group), and are being annotated currently (L. Altschmied). In addition, BAC clones from apomictic plants containing all three sexual loci known to Fig. 38 Sequence motifs in 21 promoter regions of Arabidopsis, which were enriched by contribute to the apomeiosis locus were identified (L.A ltschmied, chromatin immuno-precipitation and activated by the B3-domain transcription D. Fiedler) and will be sequenced. These sequences may help to factor ABI3 in transiently transformed protoplasts. ACTIV: ratio of reporter gene expression with and without ABI3; ABRE-like: sequence motifs with similarity to answer the question, if sexual alleles in the genome of apomictic abscisic acid response elements; RY-like: sequence motifs with similarity to the plants do accumulate mutations according to a process known as RY binding site of B3-domain transcription factors; pTSS: potential transcription start site; TRLS: translation start site. (G. Mönke, Phytoantibodies; M. Seifert, Data Muller’s ratchet. Inspection; U. Hähnel, L. Altschmied).

108 To extend our work on promoters regulated by the transcription Publications factor (TF) ABI3 and most likely, some bZIP TFs during seed development of Arabidopsis thaliana, we established cultivation Peer Reviewed Papers and protoplast transformation of an embryogenic cell culture system (S. Drießlein) available from the research group Gene 2010 Regulation (Fig. 38). Three Arabidopsis promoters were selected Hi m m e l b a c h , A., L. Liu, U. Zi e r o l d , L. Al t s c h m i e d , H. Ma u c h e r , F. Be i e r , based on their content of potential TF binding sites. Their fusions D. Mü l l e r, G. He n s e l , A. He i s e , A. Schützendübel , J. Ku m l e h n & P. with a GUS reporter gene were investigated for regulation by Sc h w e i z e r : Promoters of the barley germin-like GER4 gene ABI3 and the bZIP TFs EEL, ABI5, bZIP67, bZIP10, and bZIP53 cluster enable strong transgene expression in response to in transiently tranformed protoplasts. All three promoters pathogen attack. Plant Cell 22 (2010) 937-952. are regulated by ABI3, EEL, ABI5, and the combination of Sc h a l l a u , A., F. Ar z e n t o n , A.J. Jo h n s t o n , U. Hä h n e l , D. Ko s z e g i , F. bZIP10 and 53, but not by bZIP10 or 53 alone (S. Drießlein, L. Bl a t t n e r , L. Al t s c h m i e d , G. Ha b e r e r , G. Ba r c a c c i a & H. Bä u m l e i n : Altschmied). One of the selected promoters does not contain Identification and genetic analysis of the APOSPORY locus any recognisable RY element, the only confirmed binding in Hypericum perforatum L. Plant J. 62 (2010) 773-784. site for ABI3, still it is regulated by ABI3. Currently, potential binding sites for bZIP factors in this promoter are mutagenised 2011 (D. Fiedler, S. Drießlein, L. Altschmied) to investigate, if ABI3 Kő s z e g i , D., A.J. Jo h n s t o n , T. Ru t t e n , A. Cz i h a l , L. Al t s c h m i e d , J. regulation depends on their presence and may be mediated by Ku m l e h n , S.E. Wu s t , O. Kirioukhova, J. Gh e y s e l i n c k , U. Grossniklaus promoter bound bZIP TFs. & H. Bä u m l e i n : Members of the RKD transcription factor family induce an egg cell-like gene expression program. Plant J. 67 (2011) 280-291.

109 Abteilung Molekulare Genetik/Department of Molecular Genetics

Research Group: Plant Bioinformatics Head: Prof. Falk Schreiber

Scientists related gene expression data for Boechera holboellii and Mus musculus (C. Klukas, H. Mehlhorn with research group Apomixis IPK financed and MLU Halle-Wittenberg), and integrating multimodal data Baghalian, Kambiz, Dr. (0.50, since 01.12.2011) to identify metabolic processes and key genes that correlate Hartmann, Anja (15.09.2010-28.02.2011) positive with high biomass production for Zea mays (A. Hartmann Klukas, Christian, Dr. (till 30.04.2010) with research group Bioinformatics and Information Technology, Mehlhorn, Hendrik (Pakt für Forschung und Innovation, University Erlangen-Nürnberg, and University Düsseldorf). since 01.03.2011) In addition, several add-ons of VANTED have been developed: Rohn, Hendrik (01.03.-30.11.2011) Hive (H. Rohn, www.vanted.org/hive) combines manyfold biological data types and supports the integration of spatial, Grant Positions structural, and kinetic models and data. Application examples in- Czauderna, Tobias (BMBF) clude exploring image databases using biological networks, and Grafahrend-Belau, Eva (0.50/0.75/1.00 BMBF) investigating multimodal data of Hordeum vulgare (with research Hartmann, Anja (BMBF, till 14.09.2010; since 01.03.2011) groups Heterosis and Systems Biology). SBGN-ED (T. Czauderna, Junker, Astrid, Dr. (1.00/0.50 BMBF) www.sbgn-ed.org) supports the Systems Biology Graphical No- Klapperstück, Matthias (Industry, since 01.04.2010) tation (SBGN, www.sbgn.org) and allows the creation and editing Mehlhorn, Hendrik (BMBF, till 28.02.2011) of all three types of SBGN maps, the validation of their syntactical Rohn, Hendrik (BMBF, till 28.02.2011) and semantical correctness, and the translation of already existing non-SBGN diagrams into SBGN. In 2010 and 2011 SBGN- Visiting Scientists/Scholars ED won first prizes for the best SBGN software support in the an- Asgari, Yasdan (University of Tehran, 22.06.-21.08.2011) nual SBGN competition. SBGN-ED has been extensively used for Baghalian, Kambiz, Dr. (self-financed, 03.04.-30.11.2011) knowledge representation in biology, such as the RIMAS portal Masoudi-Nejad, Ali, Prof. (DAAD, 01.08.-10.09.2010) for Arabidopsis thaliana, MetaCrop for several plant species, as Schlüter, Urte, Dr. (University of Erlangen/IPK, well as a map about plant metabolism which was awarded first 18.07.-21.07.2010) prize for the best SBGN map in the 2010 SBGN competition. Cen- Wybrow, Michael, Dr. (Monash University, 19.08.-27.08.2010) tiLib (J. Gräßler, D. Koschützki, http://centilib.ipk-gatersleben. de) is a library for computing and investigating weighted and unweighted centralities in biological networks. CentiLib provided the algorithmical environment for investigating the struc- Goals ture of metabolic and gene regulatory networks in E. coli. NETS (H. Mehlhorn) is an easy to use system for integrating biological Representing, analysing, modelling and visualising biological networks from various data sources and different domains. networks and related data; visual analytics of multimodal The flexible identifier mapping support includes synonyms biological data. and transitive identifier mapping paths. Experimental data can be mapped and analysed in related pathways, a methodology used, for example, to investigate differentially expressed genes Research Report in Zea mays and Arabidopsis thaliana (H. Mehlhorn, A. Junker, A. Hartmann with research groups Heterosis and Bioinformatics We continued the development of our open source system and Information Technology, University Erlangen-Nürnberg, VANTED (H. Rohn, C. Klukas, www.vanted.org, now available and University Düsseldorf). FluxMap (H. Rohn, www.vanted. in version 2.0) for analysing experimental data in the context of org/fluxmap, cooperation with research group Systems biological networks. Novel algorithms allow easy data integration, Biology) enables users to easily import and visualise simulated exploration and analysis, for example, to integrate metabolic and measured flux data in the context of biological networks. and regulatory knowledge or to identify over-representation of Interactions enable switching between and comparing of significantly expressed genes in pathways.ANTE V D has been different flux distributions for different species, or even enrich used in many in-house and external collaborations such as these flux maps by other *omics data. investigating metabolism in Hordeum vulgare and Brassica napus The development of the MetaCrop information system (E. Grafahrend-Belau, M. Klapperstück with research groups for metabolic pathway information has been continued (M. Heterosis, Molecular Plant Nutrition, Systems Biology, MLU Klapperstück, metacrop.ipk-gatersleben.de, cooperation with Halle-Wittenberg, Bayer CropScience, and SunGene), analysing research groups Bioinformatics and Information Technology metabolic and regulatory networks in Beta vulgaris (H. Mehlhorn and Systems Biology), and MetaCrop 2.0 has been released. New with research group Applied Biochemistry), exploring pathway- features include web services, private data spaces, SBGN support,

110 Fig. 39 SBGN for visual analysis of data in the context of (top) metabolic network (plant central metabolism with metabolite and expression data) and (bottom) regulatory network (ABCDE model of Arabidopsis thaliana flower development with expression data) using MetaCrop, Vanted, SBGN-ED and HIVE (A. Hartmann, A. Junker, H. Rohn, T. Czauderna).

and direct access from VANTED. Based on intensive surveys of and related methods and allow predicting flux distributions and scientific literature, the MetaCrop content has been extended optimal metabolic capabilities under different environmental to detailed information about 62 pathways based on over 1,800 conditions and genetic backgrounds. An example is the simulation publications (E. Grafahrend-Belau, A. Hartmann, A. Junker cf. Fig. of endosperm metabolism in Jekyll down-regulated plants and 39). In addition, the DBE2 information system for managing Risø13 mutants to elucidate the maternal and filial effects on biological experiment data has been further developed (H. sugar allocation within the barley seed, where model predictions Mehlhorn) and now provides web services and a seamless gave new insights into the response of seed storage metabolism integration into VANTED. DBE2 is used in several in-house and to variation in the supply of sucrose. Mathematical methods and external collaborations for managing experimental data which is models for multiscale modelling of metabolism combining analysed with the Vanted system. different modelling approaches have been further developed. Information in MetaCrop is the basis for stoichiometric models, For an example in Hordeum vulgare, three stoichiometric models and several stoichiometric, compartmented models have been representing seed, source leaf, and culm have been generated developed and analysed, such as developing endosperm of (E. Grafahrend-Belau, A. Junker, cooperation with research group Hordeum vulgare, developing embryo of Medicago truncatula, Systems Biology) and will be coupled, including parameters developing embryo of Pisum sativum and source leaf in Zea mays derived from a barley household model, to develop strategies

(E. Grafahrend-Belau, A. Hartmann, A. Junker with research groups aiming at the increase of the efficiency of CO2 assimilation, solar Heterosis and Systems Biology). The models provide frameworks energy fixation, and biomass production. for studying metabolism in silico with flux balance analysis (FBA)

111 Abteilung Molekulare Genetik/Department of Molecular Genetics

In an international collaboration with various partners the Systems Publications Biology Graphical Notation (SBGN), representing a standard for the display and handling of biological networks, has been further Peer Reviewed Papers developed. Several tools and resources were established in the group which will facilitate and exemplify the use of SBGN for 2010 biologists such as SBGN-ED, MetaCrop SBGN-pathways, RIMAS, Ba n a e i Mo g h a d d a m , A.M., J. Fu c h s , T. Cz a u d e r n a , A. Ho u b e n & M.F. SBGN bricks, and LibSBGN. RIMAS is a web-based information Me t t e : Intraspecific hybrids of Arabidopsis thaliana revealed portal which provides a comprehensive overview of regulatory no gross alterations in endopolyploidy, DNA methylation, pathways and genetic interactions during Arabidopsis thaliana histone modifications and transcript levels. Theor. Appl. embryo and seed development (A. Junker, with research group Genet. 120 (2010) 215-226. Gene Regulation). The brick concept (adapted from Synthetic Cz a u d e r n a , T., C. Kl u k a s & F. Sc h r e i b e r : Editing, validating, and Biology) has been transferred to SBGN (A. Junker). Fundamental translating of SBGN maps. Bioinformatics 26 (2010) 2340-2341. biological patterns are translated into a limited number of Hi p p e , K., C. Co l m s e e , T. Cz a u d e r n a , E. Gr a f a h r e n d -Be l a u , B.H. Ju n k e r , re-useable SBGN bricks for assembly into larger networks. A C. Kl u k a s , U. Sc h o l z , F. Sc h r e i b e r & S. We i s e : Novel developments dictionary comprising an initial set of bricks has been generated of the MetaCrop information system for facilitating systems and will be available for download. The use of SBGN bricks will biological approaches. J. Integr. Bioinform. 7 (2010) e125. simplify the way biologists can work with SBGN. Ju n k e r , A., A. Ha r t m a n n , F. Sc h r e i b e r & H. Bä u m l e i n : An engineer’s view In collaboration with external partners several novel algorithms on regulation of seed development. Trends Plant Sci. 15 (2010) for structural network analysis and automatic network 303-307. layout (D. Koschützki, T. Czauderna, H. Rohn) and very efficient Kl u k a s , C. & F. Sc h r e i b e r : Integration of -omics data and networks algorithms using parallel architectures such as the Cell for biomedical research with VANTED. J. Integr. Bioinform. 7 processor or GPGPUs have been developed. For the non- (2010) 112. destructive high-throughput phenotyping system LemnaTec Ko s c h ü t z k i , D., B.H. Ju n k e r , J. Sc h w e n d e r & F. Sc h r e i b e r : Structural we continued the development of the image analysis pipeline analysis of metabolic networks based on flux centrality. J. HTPheno (htpheno.ipk-gatersleben.de) to compute phenotypic Theor. Biol. 265 (2010) 261-269. parameters such as plant height, plant width, and leaf area from Sc h a r f e , M., R. Pi e l o t & F. Sc h r e i b e r : Fast multi-core based multimodal images (A. Hartmann). The results of the analysis have been used registration of 2D cross-sections and 3D datasets. BMC in projects to derive biological insights, such as in drought stress Bioinformatics 11 (2010) 20. during plant development in Hordeum vulgare (A. Hartmann with Sh a r b e l , T.F., M.L. Vo i g t , J.M. Co r r a l , G. Ga l l a , J. Ku m l e h n , C. Kl u k a s , research group Genome Diversity). The development of image F. S c h r e i b e r , H. Vo g e l & B. Ro t t e r : Apomictic and sexual ovules analysis algorithms has been transferred to the newly established of Boechera display heterochronic global gene expression research group Image Analysis (C. Klukas). patterns. Plant Cell 22 (2010) 655-671. We i d e m a n n , W., C. Kl u k a s , A. Kl e i n , A. Si m m , F. Sc h r e i b e r & R. Ho r s t k o r t e : Lessons from GNE-deficient embryonic stem cells: Sialic acid biosynthesis is involved in proliferation and gene expression. Glycobiology 20 (2010) 107-117.

2011 Ha r t m a n n , A., T. Cz a u d e r n a , R. Ho f f m a n n , N. St e i n & F. Sc h r e i b e r : HTPheno: An image analysis pipeline for high-throughput plant phenotyping. BMC Bioinformatics 12 (2011) 148. Me h l h o r n , H. & F. Sc h r e i b e r : DBE2 – management of experimental data for the VANTED system. J. Integr. Bioinform. 8 (2011) 162. Me l k u s , G., H. Ro l l e t s c h e k , J. Fu c h s , V. Ra d c h u k , E. Gr a f a h r e n d -Be l a u , N. Sreenivasulu , T. Ru t t e n , D. We i e r , N. He i n z e l , F. Sc h r e i b e r , T. Al t m a n n , P.M. Ja k o b & L. Bo r i s j u k : Dynamic (13) C/(1) H NMR imaging uncovers sugar allocation in the living seed. Plant Biotechnol. J. 9 (2011) 1022-1037. Ro h n , H., C. Kl u k a s & F. Sc h r e i b e r : Creating views on integrated multidomain data. Bioinformatics 27 (2011) 1839-1845. Ro h n , H., C. Kl u k a s & F. Sc h r e i b e r : Visual analytics of multimodal biological data. Proceedings of the International Conference on Information Visualization Theory and Applications (IVAPP’11). SciTePress (2011) 256-261.

112 Ro l l e t s c h e k , H., G. Me l k u s , E. Gr a f a h r e n d -Be l a u , J. Fu c h s , N. He i n z e l , F. Pu c k n a t , K., H. Ro h n & F. Sc h r e i b e r : Anaglyph Canvas3D. https:// Sc h r e i b e r , P.M. Ja k o b & L. Bo r i s j u k : Combined noninvasive imaging sourceforge.net/projects/anaglyphcanvas3 (2010). and modeling approaches reveal metabolic compartmentation Ro h n , H., C. Kl u k a s & F. S c h r e i b e r : HIVE. http://vanted.ipk-gatersleben. in the barley endosperm. Plant Cell 23 (2011) 3041-3054. de/addons / hive (2010). We i s e , S., J. Ch e n , B.H. Ju n k e r , M. Klapperstück , M. La n g e , F. Sc h r e i b e r & U. We i s e , S., I. Gr o s s e , C. Kl u k a s , D. Ko s c h ü t z k i , U. Sc h o l z , F. Sc h r e i b e r & B.H. Sc h o l z : Das Informationssystem MetaCrop zur Unterstützung Ju n k e r : Meta-All: A system for managing metabolic pathway systembiologischer Forschung an Kulturpflanzen. information. http://meta-all.ipk-gatersleben.de (2010). it – Information Technology 53 (2011) 234-240. 2011 Cz a u d e r n a , T. & F. Sc h r e i b e r : SBGN-ED. http://www.sbgn-ed.org Books and Book Chapters (2011). Ro h n , H., C. Kl u k a s & F. Sc h r e i b e r : Vanted 2.0. http://www.vanted. 2010 org (2011). Al b r e c h t , M., A. Ke r r e n , K. Kl e i n , O. Ko h l b a c h e r , P. Mu t z e l , W. Pa u l , Sc h r e i b e r , F., C. Co l m s e e , T. Cz a u d e r n a , E. Gr a f a h r e n d -Be l a u , A. Ha r t m a n n , F. Sc h r e i b e r & M. Wy b r o w : On open problems in biological A. Ju n k e r , B.H. Ju n k e r , D. Ko s c h ü t z k i , U. Sc h o l z & S. We i s e : MetaCrop network visualization. In: Ep p s t e i n , D. & E.R. Ga n s n e r (Eds.): 2.0. http://metacrop.ipk-gatersleben.de (2011). Graph Drawing, Proc. 17th Int. Symp. on Graph Drawing, v a n Ie r s e l , M.P., S.E. Bo y d , F. Be r g m a n n , S. Mo o d i e , F. Sc h r e i b e r , T. Cz a u - LNCS, Vol. 5849. Springer (2010) 256-267. d e r n a , E. De m i r , N. Le No v è r e , A. So r o k i n , H. Mi, A. Lu n a , U. Do g r u - Sc h r e i b e r , F.: Representation and analysis of biological networks s o z , Y. Ma t s u o k a , A. Fu n a h a s h i , H. Ki t a n o , M.I. Al a d j e m , M.L. Bl i n o v & with the Vanted toolsuite. In: Ho f e s t ä d t , R. & N. Ko l c h a n o v A.C. Vi l l é g e r : LibSBGN. http://libsbgn.sourceforge.net (2011). (Eds.): German/Russian Network of Computational Systems We i s e , S., I. Gr o s s e , C. Kl u k a s , D. Ko s c h ü t z k i , U. Sc h o l z , F. Sc h r e i b e r & B.H. Biology, Berichte aus der Medizinischen Informatik und Ju n k e r : Meta-All: A system for managing metabolic pathway Bioinformatik. Shaker Verlag (2010) 47-50. information. http://meta-all.ipk-gatersleben.de (2011). Sc h r e i b e r , F. & H. Schwöbbemeyer : Motifs in biological networks. In: St u m p f , M. & C. Wi u f (Eds.): Statistical and Evolutionary Analysis of Biological Network Data. Imperial College Press/ PhD and Diploma Theses World Scientific (2010) 45-64. 2010 Gr ä s s l e r , J.: Optimierung und Weiterentwicklung von Algorith- 2011 men und einer Bibliothek zur Berechnung von Netzwerk- Ko c h , I., W. Re i s i g & F. Sc h r e i b e r : Modeling in systems biology: the Zentralitäten. (Diploma Thesis) Martin-Luther-Universität petri net approach. Springer, NY, USA (2011) 364 pp. Halle-Wittenberg, Halle/S. (2010) 59 pp.

2011 Other Papers Fr ö h l i c h , S.: Modellprüfung biologischer Systeme. (Diploma 2010 Thesis) Martin-Luther-Universität Halle-Wittenberg, Halle/S. Le m k e , R., M. Ha j i r e z a e i , B.H. Ju n k e r , J. Mü l l e r, B. Us a d e l , M. Le p s & (2011) 100 pp. F. Sc h r e i b e r : MMM – Mehrskalen-Stoffwechselmodelle zur Ko s c h ü t z k i , D.: Zentralitätsanalyse molekularbiologischer Netz- Systembiologie in Getreiden – ein integrativer Ansatz für die werke. (PhD Thesis) Martin-Luther-Universität Halle-Witten- Biomasseforschung. Systembiologie.de 2 (2010) 51-53. berg, Halle/S. (2011) 110 pp. Sc h ö n h e r r , I.S.: Assemblierung und Analyse des Vitis vinifera- Genoms der Rebsorte Riesling. (Bachelor Thesis) Martin- Electronic Publications Luther-Universität Halle-Wittenberg, Institut für Informatik, 2010 Halle/S. (2011) 40 pp. Cz a u d e r n a , T., C. Kl u k a s & F. Sc h r e i b e r : SBGN-ED. http://vanted.ipk- Vo g t , T.: Entwicklung und Implementierung eines Algorithmus gatersleben.de/addons/sbgn-ed (2010). zur Übersetzung von SBGN Process Description Diagrammen Gr a f a h r e n d -Be l a u , E., S. We i s e , D. Ko s c h ü t z k i , U. Sc h o l z , B.H. Ju n k e r in SBGN Activity Flow Diagramme. (Diploma Thesis) Martin- & F. Sc h r e i b e r : MetaCrop – A detailed database of crop plant Luther-Universität Halle-Wittenberg, Halle/S. (2011) 62 pp. metabolism. http://metacrop.ipk-gatersleben.de (2010). Gr ä s s l e r , J., D. Ko s c h ü t z k i & F. Sc h r e i b e r : CentiLib. http://centilib.ipk- gatersleben.de (2010). Additional Publications 2009 Ha r t m a n n , A., T. Cz a u d e r n a & F. Sc h r e i b e r : HTPheno. http://htpheno. Kl u k a s , C. & F. Sc h r e i b e r : Integration of -omics data and networks ipk-gatersleben.de (2010). for biomedical research. Proc. Intl. Workshop Computational Kl u k a s , C. & F. Sc h r e i b e r : VANTED. http://vanted.ipk-gatersleben.de and Integrative Biology – CIB2009, 18.-20.09.2009, Hangzhou, (2010). China (2009) 14-19. Me h l h o r n , H. & F. Sc h r e i b e r : DBE2. http://vanted.ipk-gatersleben. Om i d i , S., F. Sc h r e i b e r & A. Ma s o u d i -Ne j a d : MODA: An efficient al- de/addons/DBE2 (2010). gorithm for network motif discovery in biological networks. Genes Genet. Syst. 84 (2009) 385-395.

113 Abteilung Molekulare Genetik/Department of Molecular Genetics

Research Group: Data Inspection Head: Dr. Marc Strickert (till 31 January 2010) Head: Dr. Swetlana Friedel (since 1 February 2010)

Scientists methods. The DI group takes a leading role in analysing those sets with modern probabilistic modeling approaches in close Grant Positions cooperation with biological groups inside and outside of the IPK. Keilwagen, Jens, Dr. (Saxony-Anhalt) In addition to advanced data analyses for different IPK groups, Seifert, Michael, Dr. (Saxony-Anhalt) the main focus of the DI group is on the following areas: (1) computational genome comparison, (2) motif discovery, (3) reverse engineering, and (4) data mining in the cereals collection of the IPK Genebank. Goals (1) By combining the complementary strengths in epigenetics Processing, visualisation and interpretation of high-dimensional of Dr. Vincent Colot’s group (Institut de Biologie de l’Ecole biological data with modern machine learning methods. Normale Supérieure, France) with machine learning techniques of our group, the aim is to identify newly inserted transposable elements (TEs). First microarray experiments are already done for Research Report investigating the DNA methylation status of TEs in a population of epigenetic recombinant inbred lines (epiRILs) of Arabidopsis The Data Inspection (DI) research group financed by the Ministry thaliana. The huge data sets resulting from these experiments of Science and Economics of Saxony-Anhalt, XP 3624HP/0606T will then be analysed with help of a new class of Hidden Markov analyses a broad spectrum of high-throughput biological data Models developed by M. Seifert (http://www.jstacs.de/index. sets. Our work begins where very large and/or heterogeneous php/PHHMM) within the frame of a DAAD bilateral project. data cannot be processed any more using standard statistical

Fig. 40 The whole Genebank collection of spring barley accessions is represented in a 3-dimensional cube, where each dimension corresponds to one of three selected traits: plant height, thousand grain weight and days to flowering.T hose traits are normalised according to our new statistical ranking method. This enables us to find an accession with early flowering, small plant height and high thousand grain weight, for example (indicated by the star). On the right site histograms of raw data for all cultivation years and for each trait are shown. (J. Keilwagen, S. Friedel, B. Kilian and H. Knüpffer)

114 (2) We developed the de novo motif discovery tool Dispom (http:// Publications www.jstacs.de/index.php/Dispom) for finding differentially abundant transcription factor binding sites. It models existing Peer Reviewed Papers positional preferences of binding sites and adjusts the length of the motif in the learning process. J. Keilwagen in cooperation 2010 with colleagues from Martin Luther University Halle was able to Ka l e t a , C., A. Gö h l e r , S. Sc h u s t e r , K. Ja h r e i s , R. Gu t h k e & S. Ni k o l a j e w a : show that the prediction performance of this tool is superior to Integrative inference of gene-regulatory networks in existing tools for de novo motif discovery. Finally, together with Escherichia coli using information theoretic concepts and the group of I. Paponov from the Albert Ludwigs University sequence analysis. BMC Syst. Biol. 4 (2010) 116. Freiburg the tool was applied for discovering binding sites Ke i l w a g e n , J., J. Gr a u , S. Po s c h & I. Gr o s s e : Apples and oranges: enriched in promoters of auxin-responsive genes. The genes avoiding different priors inB ayesian DNA sequence analysis. were extracted from A. thaliana microarray data, and a new BMC Bioinformatics 11 (2010) 149. motif was found that can be interpreted as an elongated auxin- Ke i l w a g e n , J., J. Gr a u , S. Po s c h , M. St r i c k e r t & I. Gr o s s e : Unifying responsive element. This result has been validated using an generative and discriminative learning principles. BMC independent data set of auxin-responsive genes. The refined Bioinformatics 11 (2010) 98. motif increased the auxin specificity by more than three orders Si n h a , R., T. Le n s e r , N. Ja h n , U. Ga u s m a n n , S. Fr i e d e l , K. Sz a f r a n s k i , K. of magnitude in genome-wide predictions compared to the Hu s e , P. Ro s e n s t i e l , J. Ha m p e , S. Sc h u s t e r , M. Hi l l e r, R. Ba c k o f e n & canonical auxin-responsive element. M. Pl a t z e r : TassDB2 – A comprehensive database of subtle alternative splicing events. BMC Bioinformatics 11 (2010) (3) With help of reverse engineering we reconstructed gene 216. regulatory networks in A. thaliana under varying conditions for Sreenivasulu , N., V. Ra d c h u k , A. Al a w a d y , L. Bo r i s j u k , D. We i e r , N. transcriptomic network comparison. For that we used modern St a r o s k e , J. Fu c h s , O. Mi e r s c h , M. St r i c k e r t , B. Us a d e l , U. Wo b u s , information-theoretic approaches and integrated sequence B. Gr i m m , H. We b e r & W. We s c h k e : De-regulation of abscisic acid analysis. Our first case study at theI PK was done together with L. contents causes abnormal endosperm development in the Borisjuk and H. Rolletschek (research group Heterosis). We were barley mutant seg8. Plant J. 64 (2010) 589-603. able to show that deviations in gene regulatory interactions We i c h e r t , N., I. Sa a l b a c h , H. We i c h e r t , S. Ko h l , A. Er b a n , J. Ko p k a , B. due to AtHb1-overexpression point to alterations in cell wall Ha u s e , A. Va r s h n e y , N. Sreenivasulu , M. St r i c k e r t , J. Ku m l e h n , W. metabolism (Thiel et al. 2011). We s c h k e & H. We b e r : Increasing sucrose uptake capacity of wheat grains stimulates storage protein synthesis. Plant (4) Armed with a toolkit of modern probabilistic methods, we Physiol. 152 (2010) 698-710. recently focused our research on the historical IPK Genebank collection of cereals reaching back as far as 1946. The collection 2011 is one of the most important genetic resources of cereals for Ba n a e i Mo g h a d d a m , A.M., F. Ro u d i e r , M. Se i f e r t , C. Be r a r d , M.L. research and breeding. It contains a very large collection of Ma g n i e t t e , R. Ka r i m i As h t i y a n i , A. Ho u b e n , V. Co l o t & M.F. Me t t e : barley and wheat accessions (~ 50,000) which we analysed first. Additive inheritance of histone modifications in Arabidopsis To overcome technical and climatic biases we developed a new thaliana intra-specific hybrids. Plant J. 67 (2011) 691-700. statistical approach for ranking the accessions. With help of this Ke i l w a g e n , J., J. Gr a u , I.A. Pa p o n o v , S. Po s c h , M. St r i c k e r t & I. Gr o s s e : method we now can, for example, identify an optimal barley De-novo discovery of differentially abundant transcription accession for any desired trait (see Fig. 40, p. 114). Based on factor binding sites including their positional preference. such kind of analysis we defined groups of most contrasting PLoS Comput. Biol. 7 (2011) e1001070. accessions for an in-depth analysis. This was done together Se i f e r t , M., M. St r i c k e r t , A. Sc h l i e p & I. Gr o s s e : Exploiting prior with B. Kilian from the Genome Diversity group. We were able to knowledge and gene distances in the analysis of tumor prove the usefulness of our methods on groups of contrasting expression profiles with extended Hidden Markov Models. barley accessions in a breeding approach. For the future we Bioinformatics 27 (2011) 1645-1652. consider it as a big challenge to quantitatively evaluate the Se i l e r , C., V.T. Harshavardhan , K. Ra j e s h , P.S. Re d d y , M. St r i c k e r t , H. biodiversity in cereals. This would enable a more systematic Ro l l e t s c h e k , U. Sc h o l z , U. Wo b u s & N. Sreenivasulu : ABA biosynthesis and intelligent access to the plant genetic resources stored at and degradation contributing to ABA homeostasis during the IPK Genebank for scientists and breeders world-wide. barley seed development under control and terminal drought- stress conditions. J. Exp. Bot. 62 (2011) 2615-32.

115 Abteilung Molekulare Genetik/Department of Molecular Genetics

Th i e l , J., H. Ro l l e t s c h e k , S. Fr i e d e l , J.E. Lu n n , T.H. Ng u y e n , R. Fe i l , H. 2011 Ts c h i e r s c h , M. Mü l l e r & L. Bo r i s j u k : Seed-specific elevation of Ho t z e , M. & S. Fr i e d e l : dCAIoptimizer: A new tool for advanced non-symbiotic hemoglobin AtHb1: beneficial effects and codon optimization. https://www.omnifung.hki-jena.de/ underlying molecular networks in Arabidopsis thaliana. BMC Rpad/dCAIoptimizer.Rpad (2011). Plant Biol. 11 (2011) 48. Ke i l w a g e n , J., J. Gr a u , A. Go h r , B. Ha l d e m a n n , M. Se i f e r t , M. Mo h r , S. Wi t z e l , K., C. Pi e t s c h , M. St r i c k e r t , A. Ma t r o s , M. Rö d e r , W. We s c h k e , Po s c h & I. Gr o s s e : A Java framework for statistical analysis and U. Wo b u s & H.P. Mo c k : Mapping of quantitative trait loci classification of biological sequences. http://www.jstacs.de associated with protein expression variation in barley grains. (2011). Mol. Breed. 27 (2011) 301-314. Ke i l w a g e n , J., J. Gr a u , I.A. Pa p o n o v , S. Po s c h , M. St r i c k e r t & I. Gr o s s e : Dispom: de-novo discovery of differentially abundant transcription factor binding sites including their positional Books and Book Chapters preference. http://www.jstacs.de/index.php/Dispom (2011). Se i f e r t , M., M. St r i c k e r t , A. Sc h l i e p & I. Gr o s s e : Exploiting prior 2010 knowledge and gene distances in the analysis of tumor Po s c h , S., J. Gr a u , A. Go h r , J. Ke i l w a g e n & I. Gr o s s e : Probabilistic expression profiles with extended Hidden Markov Models. approaches to transcription factor binding site prediction. http://www.jstacs.de/index.php/DSHMM (2011). In: La d u n g a , I. (Ed.): Computational Biology of Transcription Factor Binding. Methods Mol. Biol. 674 (2010) 97-119. Sreenivasulu , N., R. Su n k a r , U. Wo b u s & M. St r i c k e r t : Array PhD and Diploma Theses platforms and bioinformatics tools for the analysis of plant 2010 transcriptome in response to abiotic stress. In: Su n k a r , R. (Ed.): Ho t z e , M.: In silico Sequenzoptimierung synthetischer Gene Plant Stress Tolerance – Methods and Protocols. Methods und Zuordnung des Expressionslevels über Transkriptions- Mol. Biol. 639. Humana Press (2010) 71-93. analysen am Beispiel von Candida albicans. (Diploma Thesis) Friedrich-Schiller-Universität, Jena (2010) 133 pp. Ke i l w a g e n , J.: Predicting DNA binding sites using generative, Electronic Publications discriminative, and hybrid learning principles. (PhD Thesis) 2010 Martin-Luther-Universität Halle-Wittenberg, Naturwissen- Ke i l w a g e n , J., J. Gr a u , A. Go h r , B. Ha l d e m a n n , M. Se i f e r t , M. Mo h r , S. schaftliche Fakultät III, Institut für Informatik, Halle/S. (2010) Po s c h & I. Gr o s s e : A Java framework for statistical analysis and 131 pp. classification of biological sequences. http://www.jstacs.de Se i f e r t , M.: Extensions of Hidden Markov Models for the analysis (2010). of DNA microarray data. (PhD Thesis) Martin-Luther-Univer- Op p e r m a n n , M., J. Ke i l w a g e n , H. Kn ü p f f e r , S. Fr i e d e l & A. Bö r n e r : sität Halle-Wittenberg, Naturwissenschaftliche Fakultät III, Validation, analysis and aggregation of long-term trait Institut für Informatik, Halle/S. (2010) 176 pp. observation data of genebank material. In: We i t z m a n n , A.L. (Ed.), Proceedings of TDWG (2010). Woods Hole, Massachusetts, USA (2010) 52-53. http://www.vliz.be/ imisdocs/publications/215525.pdf (2010). Si n h a , R., T. Le n s e r , N. Ja h n , U. Ga u s m a n n , S. Fr i e d e l , K. Sz a f r a n s k i , K. Hu s e , P. Ro s e n s t i e l , J. Ha m p e , S. Sc h u s t e r , M. Hi l l e r, R. Ba c k o f e n & M. Pl a t z e r : Tandem Splice Site Database, TassDB2. http:// gen100.imb-jena.de/TassDB2 (2010).

116 Research Group: Hybrid Wheat Head: Dr. Mario Gils

Scientists The transformed provector constructs enable the production of male-sterile wheat lines by in planta assembly of a barnase IPK financed protein (M. Gils, K. Kempe, M. Rubtsova). Male-sterility is stable Rubtsova, Myroslava, Dr. (0.75, 01.07.-31.08.2010) over several generations and under increased temperatures, without compromising female fertility. Since the split gene sys- Grant Positions tem invariantly requires functional single-copy integrations Kempe, Katja, Dr. (BMBF, since 01.10.2011) of the prolocus, the basal vectors were modified in several Rubtsova, Myroslava, Dr. (BMBF, till 30.06.2010; Industry, steps (M. Gils). The insertion of introns for intron-mediated 01.09.2010-31.01.2011; BMBF, since 01.02.2011) enhancement of gene expression (IME) led to a significant improvement of the split-barnase system, as been tested by transient assays in tobacco and stable transformation of wheat (M. Rubtsova, M. Gils). Three different introns were inserted Goals into two alternative positions of the C- and N-terminal coding regions and combined in various prolocus versions (examples The establishment of a transgenic pollination control system are given in see Fig. 41b). In oppose to the “first generation vec- for commercial hybrid wheat seed production. tors”, we were able to generate male-sterile wheat plants with a single-copy insertion of the prolocus. For isolocus production, a phiC31-based site-specific recombination system was Research Report established for wheat. The prolocus “substrate” was exposed to a phiC31 integrase encoded by a transgene that resides on A split-gene approach for hybrid seed production a different chromosomal locus. In 34 independent lines, DNA A precursor-T-DNA (“provector”) containing two complementary sequences between att recombination sites were excised from tapetum-expressed barnase gene fragments is transformed proloci integrated in the wheat genome. We produced recom- into plants (see Fig. 41a). Site-specific deletions of the T-DNAs binant isoloci that were transmitted to the subsequent genera- during plant development result in two alternative derivative tion (K. Kempe, M. Rubtsova, M. Gils). loci, with each producing only one of the two complementary barnase precursor proteins. Crossing of plants that carry the Phenotyping pollinators (“males”) for hybrid wheat respective complementary loci with each other leads to progeny breeding plants harbouring the two barnase gene fragments in allelic The objective is the development of a semi-automatic pheno- positions. These plants are male-sterile and are used as the typing technology for pollination capacity (jointly with LSA female crossing partners for hybrid breeding. The T1 hybrids Hohenheim, J. Reif; Nordsaat GmbH, R. Schachschneider and are fertile as the barnase gene fragments segregate in the the research group Genome Plasticity, R. Schmidt, A. Boudi- progeny. For the maintenance of the female crossing partner, chevskaia). With the goal to identify suitable “father lines“ for the heterozygous plant can be crossed to a homozygous line. hybrid breeding, pollen traps were developed and tested in Given that the gene fragments conferring male sterility are open-field trials (M. Gils, see Fig. 41c). Image analysis software linked to an herbicide tolerance gene, the heterozygous plants facilitates a high-throughput screening of pollen amount, size can be selected by applying an herbicide. The system allows and shape. By assaying several lines with different efficiency for mixed breeding of father and mother lines. In order to of pollen shedding, a technical proof-of-concept could be increase the stability of the barnase protein complex, barnase achieved. gene fragments have been fused to intein sequences that, upon translation, covalently fuse the protein fragments auto- Production of doubled haploid winter wheat catalytically in a process called protein-splicing. An anther culture-based technology for winter wheat was developed that regenerated high numbers of green and fertile doubled haploid plants (DHs) from a colchicine-free anther- culture approach with a frequency of >60 % (M. Rubtsova, in collaboration with Saaten-Union Biotec GmbH, H. Gnad, J. Weyen). Our results indicate that the external application of auxins in the induction media plays an important role for the ploidy switch.

117 Abteilung Molekulare Genetik/Department of Molecular Genetics

Fig. 41 a) The split-gene approach for hybrid seed production. Tapetum-specific promoter. b)A single-copy prolocus integration results in male-sterile wheat plants. c) Measurement of pollination capacities under open-field conditions and analysis of pollen size and shape (M. Gils).

Further technologies and tools developed in collaborative Publications activities The intein technology was used for the production of spider silk Peer Reviewed Papers protein multimers in transgenic tobacco plants (jointly with U. Conrad, see report of the research group Phytoantibodies). 2010 Furthermore, new putative intein sequences have been Ke m p e , K., M. Ru b t s o v a , C. Be r g e r , J. Ku m l e h n , C. Sc h o l l m e i e r & M. Gi l s : identified by a database screen (in collaboration with the Transgene excision from wheat chromosomes by phage research group Plant Bioinformatics, F. Schreiber, H. Mehlhorn). phiC31 integrase. Plant Mol. Biol. 72 (2010) 673-687. We cloned vectors for transient assays that allow a rapid validation of the candidate sequences. 2011 Functional phiC31-based recombination systems were Ke m p e , K. & M. Gi l s : Pollination control technologies for hybrid established for barley (M. Rubtsova, in collaboration with the breeding. Mol. Breed. 27 (2011) 417-437. research group Plant Reproductive Biology, J. Kumlehn, E. Kapusi). A site-specific transgene integration system that is based on interspecific hybrids (wheat × pearl millet) is currently under development (in collaboration with Saaten-Union Biotec, J. Weyen).

118 Research Group: Image Analysis (since 1 May 2010) Head: Dr. Christian Klukas

Scientists Automated image analysis functions have been implemented within IAP to access barley and maize plant growth (height, IPK financed width, projected area, digital biomass and more) as well as Entzian, Alexander (0.50/1.00, since 15.09.2010) to characterise relative differences in plant photosynthesis and water content by calculating intensity histograms for foreground (plant) pixels of the fluorescence and near-infrared images. The foreground/background separation has been Goals implemented appropriately by developing new automated calibration procedures which consider and correct for the The research group Image Analysis focused on the development fluctuating positions of marker points, colour temperatures and establishment of an information system for the storage and noise levels. The robustness and accuracy of the image and analysis of high-throughput image data. The available analysis procedures for barley and maize have been validated infrastructure for the automated movement and imaging in the frame of two test runs in the automated greenhouse, of plants enables researchers to collect huge amounts of suitable for maize plants. image data (captured are visible light, near-infrared spectra Image analysis results may be exported as data tables or and fluorescence). Newly developed or adapted image accessed using automatically generated reports (PDF files), analysis and data integration methods as well as the usage providing parameter descriptions and diagrams showing of existing software packages enable to support researchers parameter value changes over time for the different treatments in investigating developmental changes and differences in and genotypes in the particular experiment under investigation. selected phenotypic properties of plants. These report files are generated on demand using the statistics tool ‘R’ as well as the document preparation system ‘LaTeX’. The IAP system incorporates the open source VANTED system and Research Report the publicly available open source image-processing library ImageJ as core libraries (C. Klukas and A. Entzian). In 2010 and 2011 the group worked on establishing and developing the foundation for the automatic analysis of During 2010 and 2011 the collaboration with Prof. M. Chen image datasets, derived from three different camera systems from the College of Life Sciences, Zhejiang University, China (visible light, fluorescence, near-infrared).T he first step was the has been further developed. The support in form of a travel and analysis of existing data structures in the LemnaTec database research grant from the National Natural Science Foundation system and development of an access module that transforms of China (NSFC), under the frame of the “Research Fund for these data sets on-demand into a form, suitable for the image International Young Scientists” made it possible to work on the analysis within the “Integrated Analysis Platform” (IAP). The project “Joint development and improvement of bioinformatic newly developed Java-based information system IAP serves tools for storage, integration and analysis of biological as a hub, providing its users multiple, easily via a web browser pathways and network-integrated visualisation of experiment accessible functions for (1) investigating captured images, data”. Within this collaboration two joint publications have weight- and watering data sets within the LemnaTec database been completed and future joint research projects have been system, (2) transferring such data for long-term archival into drafted (C. Klukas). the hierarchical storage management system (HSM) which is operated by the Bioinformatics and Information Technology In collaboration with Prof. F. Schreiber and the group Plant group (BIT), (3) downloading complete data sets onto the Bioinformatics (PBI) a number of projects, for example dealing researchers PCs and to (4) initiate the automated image analysis with multi-omics data integration (HIVE) and advanced data of experiments. exploration techniques have been completed and resulted in joint publications (C. Klukas).

119 Abteilung Molekulare Genetik/Department of Molecular Genetics

Publications Other Papers 2010 Peer Reviewed Papers Hu a n g , D., Y. Hu a n g , C. Kl u k a s , R. Ho f e s t ä d t & M. Ch e n : PBSK Browser: navigate biological pathways of PSI-MI, BioPAX, SBML 2010 and KGML formats. Proceedings of the IEEE International Cz a u d e r n a , T., C. Kl u k a s & F. Sc h r e i b e r : Editing, validating, and Conference on Bioinformatics and Biomedicine Workshops translating of SBGN maps. Bioinformatics 26 (2010) 2340- (BIBMW 2010), 18.-21.12.2010, Hongkong, China (2010) 13- 2341. 18. Hi p p e , K., C. Co l m s e e , T. Cz a u d e r n a , E. Gr a f a h r e n d -Be l a u , B.H. Ju n k e r , C. Kl u k a s , U. Sc h o l z , F. Sc h r e i b e r & S. We i s e : Novel developments Electronic Publications of the MetaCrop information system for facilitating systems 2010 biological approaches. J. Integr. Bioinform. 7 (2010) e125. Cz a u d e r n a , T., C. Kl u k a s & F. Sc h r e i b e r : SBGN-ED. http://vanted.ipk- Kl u k a s , C. & F. Sc h r e i b e r : Integration of -omics data and networks gatersleben.de/addons/sbgn-ed (2010). for biomedical research with VANTED. J. Integr. Bioinform. 7 Kl u k a s , C. & F. Sc h r e i b e r : VANTED. http://vanted.ipk-gatersleben. (2010) 112. de (2010). Sh a r b e l , T.F., M.L. Vo i g t , J.M. Co r r a l , G. Ga l l a , J. Ku m l e h n , C. Kl u k a s , Ro h n , H., C. Kl u k a s & F. Sc h r e i b e r : HIVE. http://vanted.ipk- F. Sc h r e i b e r , H. Vo g e l & B. Ro t t e r : Apomictic and sexual ovules gatersleben.de/addons/hive (2010). of Boechera display heterochronic global gene expression We i s e , S., I. Gr o s s e , C. Kl u k a s , D. Ko s c h ü t z k i , U. Sc h o l z , F. Sc h r e i b e r patterns. Plant Cell 22 (2010) 655-671. & B.H. Ju n k e r : Meta-All: A system for managing metabolic We i d e m a n n , W., C. Kl u k a s , A. Kl e i n , A. Si m m , F. Sc h r e i b e r & R. Ho r s t k o r t e : pathway information. http://meta-all.ipk-gatersleben.de Lessons from GNE-deficient embryonic stem cells: Sialic acid (2010). biosynthesis is involved in proliferation and gene expression. Glycobiology 20 (2010) 107-117. 2011 Ro h n , H., C. Kl u k a s & F. Sc h r e i b e r : Vanted 2.0. http://www.vanted. 2011 org (2011). Hu a n g , D., Y. Hu a n g , Y. Ba i , D. Ch e n , R. Ho f e s t ä d t , C. Kl u k a s & M. Ch e n : We i s e , S., I. Gr o s s e , C. Kl u k a s , D. Ko s c h ü t z k i , U. Sc h o l z , F. Sc h r e i b e r MyBioNet: interactively visualise, edit and merge biological & B.H. Ju n k e r : Meta-All: A system for managing metabolic networks on the Web. Bioinformatics 27 (2011) 3321-3322. pathway information. http://meta-all.ipk-gatersleben.de Ro h n , H., C. Kl u k a s & F. Sc h r e i b e r : Creating views on integrated (2011). multidomain data. Bioinformatics 27 (2011) 1839-1845. Ro h n , H., C. Kl u k a s & F. Sc h r e i b e r : Visual analytics of multimodal biological data. Proceedings of the International Conference on Information Visualization Theory and Applications (IVAPP’11). SciTePress (2011) 256-261.

120 Research Group: Stress Genomics (since 1 November 2010) Head: Dr. Nese Sreenivasulu

Scientists The BC3 double haploid Hordeum spontaneum 584 (BC3 DH Hs584) population containing 70 introgression lines was IPK financed assessed for its performance (yield, thousand grain weight and Venkatasubbu, Thirulogachandar (0.50, since 17.04.2011) starch content) under terminal drought and a total of 28 major QTLs (LOD score ≥3) with the hot-spot QTL on chromosome Grant Positions 1, 2 and 3 were detected (K. Rajesh in collaboration with M. Govind, Geetha, Dr. (1.00/0.75 BMBF, since 29.11.2010) Röder, research group Gene and Genome Mapping). Better Harshavardhan, Vokkaliga Thammegowda (0.50 BMBF, performing introgression lines possessing a segment of the 01.11.-31.12.2010; since 01.07.2011) Hs584 introgression in the hot-spot QTL region (depicting Kalladan, Rajesh (0.50 Industry, since 01.04.2011) minimised yield loss under terminal drought) and sensitive Seiler, Christiane, Dr. (0.75 BMBF, since 01.05.2011) introgression lines (with severe yield penalty under terminal drought) have been selected to study differential responses Visiting Scientists/Scholars of drought tolerance by throughput genomics platform. It Palakolanu, Sudhakar Reddy, Dr. (DAAD, since 06.11.2011) includes transcriptome, metabolome and enzymatic analyses Prasad, Manoj, Dr. (DLR, 18.11.-27.11.2010) together with sophisticated bioinformatic tools, which enabled Seiler, Christiane, Dr. (self-financed, 01.11.2010-30.04.2011) the identification of favourable alleles and regulatory networks Strickert, Marc, Dr. (self-financed, since 01.11.2010) underlying improved performance (V.T. Harshavardhan, K. Rajesh, M. Strickert in collaboration with H. Rolletschek, research group Heterosis).

Goals We have used a stay-green type and a normally senescing type from elite commercial breeding lines of barley (Hordeum Revealing the molecular mechanisms of yield stability under vulgare) to investigate ABA signalling under conditions of terminal drought with focus on altered grain number and seed post-anthesis drought. The drought sensitive, senescing filling efficiency. line produces much more abscisic acid (ABA) than the stay- green type under stress conditions in its source (flag leaf) and sink (developing seeds) tissue. In the senescing line, Research Report ABA catabolism to phaseic acid (PA), dihydrophaseic acid (DPA) and ABA sequestration as glucose ester (ABA-GE) are To understand these mechanisms integrative genomics also very active under prolonged drought stress indicating approaches will be used to explore (a) natural genetic variation the higher flux of ABA production. We annotated all pertinent in barley by studying introgression line populations with wild gene/transcript sequences of barley that are potentially barley accession donor, (b) mapping populations created from involved in ABA signalling and identified enriched motifs from selected breeding lines of stay-green and remobilisation and the extracted 5’ upstream regions. The ABA receptor genes (c) GMO plants of achieving ABA homeostasis using drought- were much more responsive to drought in the senescing induced promoters active during the generative phase. The line than in the stay-green line acting under ABA dosage aforementioned genetic material will be used to understand dependent manner leading to altered PP2C to RCAR ratio mechanisms of spikelet fertility influencing grain number as at the transcriptional level and ABA sensitivity. The other well reaching enhanced yield stability and uncompromised genes were in general induced in both lines albeit to various seed quality during grain filling under drought stress. extents. Notably, PKABA1 that is known to phosphorylate the transcription factor ABF1 in wheat was highly induced by stress in the senescing line as compared to the stay-green line. Taken together, the drought sensitive and tolerant lines of barley were found to differ in their ABA homeostasis and in the ABA signalling pathway, impacting water use efficiency and finally grain weight under terminal drought (C. Seiler, see Fig. 42, p. 122).

121 Abteilung Molekulare Genetik/Department of Molecular Genetics

Fig. 42 Phenotype and yield characteristics of two contrasting barley genotypes under control and drought stress conditions. A: Plants of the stay-green (left picture) and senescing (right picture) genotype after 3 weeks of stress and respective control plants. B; C: Water use efficiency (assimilation / transpiration) and stomatal conductance of the two genotypes measured in flag leaf at 12 and 16 days after flowering (d). D,E : Yield and Thousand Grain Weight (TGW) calculated per plot consisting of 50 plants each. Asterisks indicate statistical significant differences between control and stress treatment with p ≤ 0.01 (**) and p ≤ 0.001 (***).T riple “a” indicates differences between stay-green and senescing line with p ≤ 0.001 (C. Seiler and K. Rajesh).

Using this contrasting “stay-green” and efficient remobilisation Further, the function of ABA in flag leaf and seeds under terminal lines, a double haploid (DH) population is created. Comprehen- drought has been tested to verify its role in drought tolerance sive screening of a DH population will result in identifying im- in GMO barley using 16 different constructs by manipulating proved barley lines with improved seed set and grain yield un- different nodes ofABA biosynthesis and degradation pathways der drought stress. By implementing complementary genomic using a set of drought inducible promoters. Transgenic barley and genetic approaches we will identify genomic regions influ- plants are developed to manipulate the levels of ABA in source encing improved seed set under drought. More specifically, the (flag leaf) and sink (developing barley grain) and to alter ABA selected contrasting lines will be characterised using genome- hypersensitivity in seed tissues using a set of drought inducible wide transcriptome, targeted metabolite and hormome profil- promoters to study its impact on seed filling efficiency under ing to define the basis of genetic networks to increase drought terminal drought. Under severe stress the transgenic lines tolerance (N. Sreenivasulu in cooperation with N. von Wirén, performed significantly better than WT with enhanced grain research group Molecular Plant Nutrition). Using the same set weight by maintaining higher relative leaf water content of DH population, we will assess the malting efficiency from and photosynthesis due to maintained ABA homeostasis. drought-stressed seed pool and identify potential regulators Taken together, the drought sensitive (WT) and tolerant (N. Sreenivasulu in cooperation with A. Graner, research group lines (GMO) of barley were found to differ in their ABA Genome Diversity). homeostasis and in the ABA signalling pathway, impacting water use efficiency and finally grain weight under terminal drought (V.T. Harshavardhan, C. Seiler in collaboration with J. Kumlehn, research group Plant Reproductive Biology).

122 Publications Se i l e r , C., V.T. Harshavardhan , K. Ra j e s h , P.S. Re d d y , M. St r i c k e r t , H. Ro l l e t s c h e k , U. Sc h o l z , U. Wo b u s & N. Sreenivasulu : ABA Peer Reviewed Papers biosynthesis and degradation contributing to ABA homeostasis during barley seed development under control 2010 and terminal drought-stress conditions. J. Exp. Bot. 62 (2011) Lo h s e , M., A. Nu n e s -Ne s i , P. Kr ü g e r , A. Na g e l , J. Ha n n e m a n n , F.M. Gi o r g i , 2615-32. L. Ch i l d s , S. Os o r i o , D. Wa l t h e r , J. Se l b i g , N. Sreenivasulu , M. St i t t , Wo r c h , S., K. Ra j e s h , V.T. Harshavardhan , C. Pi e t s c h , V. Ko r z u n , L. Ku n t z e , A.R. Fe r n i e & B. Us a d e l : Robin: an intuitive wizard application A. Bö r n e r , U. Wo b u s , M.S. Rö d e r & N. Sreenivasulu : Haplotyping, for R-based expression microarray quality assessment and linkage mapping and expression analysis of barley genes analysis. Plant Physiol. 153 (2010) 642-651. regulated by terminal drought stress influencing seed Sh a r m a , S., N. Sreenivasulu , V.T. Harshavardhan , C. Se i l e r , S. Sh a r m a , quality. BMC Plant Biol. 11 (2011) 1. Z.N. Kh a l i l , E. Ak h u n o v , S.K. Se h g a l & M.S. Rö d e r : Delineating the structural, functional and evolutionary relationships of sucrose phosphate synthase gene family II in wheat and Books and Book Chapters related grasses. BMC Plant Biol. 10 (2010) 134. Sreenivasulu , N., L. Bo r i s j u k , B.H. Ju n k e r , H.P. Mo c k , H. Ro l l e t s c h e k , U. 2010 Se i f f e r t , W. We s c h k e & U. Wo b u s : Barley grain development toward Sreenivasulu , N., R. Su n k a r , U. Wo b u s & M. St r i c k e r t : Array an integrative view. Int. Rev. Cell Mol. Biol. 281 (2010) 49-89. platforms and bioinformatics tools for the analysis of plant Sreenivasulu , N., V. Ra d c h u k , A. Al a w a d y , L. Bo r i s j u k , D. We i e r , N. transcriptome in response to abiotic stress. In: Su n k a r , R. (Ed.): St a r o s k e , J. Fu c h s , O. Mi e r s c h , M. St r i c k e r t , B. Us a d e l , U. Wo b u s , Plant Stress Tolerance – Methods and Protocols. Methods B. Gr i m m , H. We b e r & W. We s c h k e : De-regulation of abscisic acid Mol. Biol., Vol. 639. Humana Press (2010) 71-93. contents causes abnormal endosperm development in the We b e r , H., N. Sreenivasulu & W. We s c h k e : Molecular physiology of barley mutant seg8. Plant J. 64 (2010) 589-603. seed maturation and seed storage protein biosynthesis. In: We i c h e r t , N., I. Sa a l b a c h , H. We i c h e r t , S. Ko h l , A. Er b a n , J. Ko p k a , B. Pu a , E.-C. & M.R. Da v e y (Eds.): Plant Developmental Biology – Ha u s e , A. Va r s h n e y , N. Sreenivasulu , M. St r i c k e r t , J. Ku m l e h n , W. Biotechnological Perspectives, Vol. 2. Springer, Berlin (2010) We s c h k e & H. We b e r : Increasing sucrose uptake capacity of 83-104. wheat grains stimulates storage protein synthesis. Plant Physiol. 152 (2010) 698-710. Other Papers 2011 2010 Go v i n d , G., C. Se i l e r , U. Wo b u s & N. Sreenivasulu : Importance of ABA Sreenivasulu , N., M.S. Rö d e r & U. Wo b u s : Trockenstress – eine Suche homeostasis under terminal drought stress in regulating nach den Ursachen und nach neuen Wegen zur Züchtung grain filling events. Plant Signal. Behav. 6 (2011) 1228-1231. trockentoleranter Getreide. GenomXPress 4 (2010) 4-6. La t a , C., S. Jh a , V. Dixit, N. Sreenivasulu & M. Pr a s a d : Differential antioxidative responses to dehydration-induced oxidative stress in core set of foxtail millet cultivars [Setaria italica (L.)]. PhD and Diploma Thesis Protoplasma 248 (2011) 817-828. 2011 Me l k u s , G., H. Ro l l e t s c h e k , J. Fu c h s , V. Ra d c h u k , E. Gr a f a h r e n d -Be l a u , Ko c k , V.: Salinity tolerance mechanisms in barley. (Master N. Sreenivasulu , T. Ru t t e n , D. We i e r , N. He i n z e l , F. Sc h r e i b e r , T. Thesis) Wageningen UR-Plant Breeding, Wageningen/The Al t m a n n , P.M. Ja k o b & L. Bo r i s j u k : Dynamic (13) C/(1) H NMR Netherlands (2011) 44 pp. imaging uncovers sugar allocation in the living seed. Plant Biotechnol. J. 9 (2011) 1022-1037. Pu r a n i k , S., S. Jh a , P.S. Sr i v a s t a v a , N. Sreenivasulu & M. Pr a s a d : Comparative transcriptome analysis of contrasting foxtail millet cultivars in response to short-term salinity stress. J. Plant Physiol. 168 (2011) 280-287.

123 Wahlweise: Kapitel, Thema, Unterthema, Autor, Zusatzinformationen Abteilung Physiologie und Zellbiologie/ Department of Physiology and Cell Biology

Abb. 43 Ausgewählte Forschungs- themen in der Abteilung Physiologie und Zellbio- logie. Fig. 43 Selected research topics of the Department of Phy- siology and Cell Biology.

124 Abteilung Physiologie Department of Physiology und Zellbiologie and Cell Biology Leiter: Prof. Dr. Nicolaus von Wirén Head: Prof. Nicolaus von Wirén

Allgemeine Forschungsziele Research Goals Aufklärung der Regulation von Stoffwechselvorgängen und The common aim in the department is the improvement of Entwicklungsprozessen in Pflanzen und in ihren heterologen agronomically relevant traits in crops and of biotechnological Expressionssystemen, mit dem vorrangigen Ziel agronomisch applications by an improved adaptation and regulation of relevante Merkmale in Nutzpflanzen oder biotechnologische metabolic pathways and developmental processes in plants or Verfahrensweisen zu verbessern. in unicellular expression systems.

Entwicklung im Berichtszeitraum Developments in 2010 and 2011 Von den sechs Arbeitsgruppen der Abteilung Physiologie und Out of the six research groups in the Physiology and Cell Biology Zellbiologie arbeiten drei mit einem vorwiegend biochemisch- department, three groups mainly employ a biochemical-physio- physiologischen Methodenspektrum zum Metabolismus und logically oriented range of methods to investigate the metabo- Sensing von PflanzennährstoffenA ( rbeitsgruppe Molekulare lism and sensing of nutrients (Molecular Plant Nutrition group), Pflanzenernährung), zur Regulation vom Sekundärstoffwechsel the regulation of secondary metabolism and the physiological und der physiologischen Bedeutung von Sekundärmetaboliten function of secondary metabolites under stress (Applied Bio- unter Stress (Arbeitsgruppe Angewandte Biochemie) und zu chemistry group), and system properties of primary metabolism Systemeigenschaften des Primärmetabolismus (Arbeitsgruppe (Systems Biology group). Thereby, these groups also enlarge the Systembiologie). Diese Arbeitsgruppen erweitern damit auch methodological scope of physiologically and morphologically das Spektrum an physiologisch oder morphologisch erfassba- measurable traits, which are monitored in screening projects ren Merkmalen, die beim Screening verschiedener Genotypen within and outside the department. The other three research auch in Projekten anderer Abteilungen aufgenommen werden. groups primarily employ developmental and biotechnological Die anderen drei Arbeitsgruppen wenden dagegen vorwiegend methods: the Structural Cell Biology group employs light and Methoden aus der Entwicklungsbiologie und Biotechnologie electron microscopy to describe ultra-/structural changes in an: Die Arbeitsgruppe Strukturelle Zellbiologie setzt licht- und plant tissues, while the Plant Reproductive Biology group char- elektronenmikroskopische Verfahren zur Beschreibung ultra-/ acterises developmental processes which are associated with struktureller Veränderungen in Pflanzengeweben ein, während sexual or asexual reproduction for the improvement of biotech- die Arbeitsgruppe Pflanzliche Reproduktionsbiologie Entwick- nological applications. The Yeast Genetics group employs differ- lungsprozesse, die mit der sexuellen und asexuellen Fortpflan- ent yeast strains as hosts for recombinant protein production, as zung assoziiert sind, aufklärt und in die Verbesserung biotech- gene donor and biocatalysts for new biotechnological products nologischer Verfahren einbringt. Die Arbeitsgruppe Hefegenetik and as microbial components in biosensors for environmental nutzt Hefen als Wirt für die Produktion rekombinanter Proteine, monitoring or food and feed control. Moreover, arbuscular my- als Gendonor und Biokatalysator oder als mikrobielle Kompo- corrhizal fungi are used to analyse fungal-root interactions and nente für Biosensoren zur Analyse von Umweltparametern und their effect on plant growth under stress conditions. für die Überwachung von Futter- und Nahrungsmitteln. Dane- The past two years have also been used to enlarge the techni- ben analysiert sie arbuskuläre Mykorrhizapilze bezüglich ihrer cal platform in the department. Newly established approaches Interaktion mit Pflanzenwurzeln und deren Auswirkung auf das include the mineral element analysis including automated Pflanzenwachstum unter Stressbedingungen. sample digestion, ICP-OES, sector field ICP-MS and isotope ra- In den letzten beiden Jahren wurde die breite technische Platt- tio-mass spectrometry (Molecular Plant Nutrition), a spinning- form der Abteilung weiter ausgebaut. Neu etabliert wurden u.a. disc confocal laser scanning microscopy for live-cell imaging eine komplette Mineralstoffanalytik mit automatischem Pro- (Structural Cell Biology) and a MALDI-TOF system which is used benverdau, ICP-OES, Sektorfeld-ICP-MS und Isotopenverhält- for protein identification and MS-based imaging of metabolites nis-Massenspektrometrie (Ag Molekulare Pflanzenernährung), and proteins (Applied Biochemistry). A UPLC-MS/MS-based eine Konfokale Lasermikroskopie mit Spinning-Disk zum „live- platform for phytohormone analysis and two complementary cell imaging“ und ein MALDI-TOF-Gerät, das neben der Protein- LC-MS/MS-based platforms for the analysis of isotope-labeled identifizierung auch für MS-basiertesI maging genutzt wird (Ag primary metabolites are currently being set up. Angewandte Biochemie). Die Etablierung einer UPLC-MS/MS- These platforms will also be used to advance common depart- basierten Plattform zur Bestimmung von Phytohormonen und mental research projects, such as the identification of barley zwei komplementärer LC-MS/MS-basierten Plattformen zur genotypes or genes increasing salt tolerance, of secondary Analyse von isotopenmarkierten Primärmetaboliten befinden metabolites increasing nutrient efficiency or of substances and sich derzeit noch im Aufbau. processes regulating pollen embryogenesis.

125 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

Diese Plattformen wurden auch zur Bearbeitung arbeitsgrup- Some highlights that deserve particular attention are the penübergreifender Projekte in der Abteilung eingesetzt, wie following: z. B. zur Identifizierung von Gerstengenotypen und -genen, die The Molecular Plant Nutrition group has recently established a die Salztoleranz erhöhen, von Sekundärmetaboliten, die die new key area with investigations on the morphogenetic im- Mineralstoffeffizienz verbessern oder von Substanzen und Pro- pact of nutrients on plants. The focus of this research highlights zessen, die die Pollenembryogenese steuern. changes in the architecture of lateral roots under spatially and temporarily changing nutrient supplies. In Arabidopsis, the quan- Hervorzuheben sind folgende Entwicklungen: titative assessment of different root measures showed that local Mit der Untersuchung morphogenetischer Wirkungen von ammonium supply stimulates lateral root initiation, whereas lo- Nährstoffen wurde in der Arbeitsgruppe Molekulare Pflanzen- cal nitrate enhances lateral root length. Thus, ammonium and ni- ernährung ein neuer Forschungsschwerpunkt etabliert. Dabei trate shape lateral root development in a complementary man- liegt der Fokus auf Veränderungen in der Architektur von Sei- ner (Lima et al. 2010, Plant Cell). In the case of localised Fe supply, tenwurzeln bei zeitlich oder räumlich wechselndem Nährstoff- lateral roots also elongate into Fe-rich patches. This process is angebot. Über die quantitative Erfassung unterschiedlicher triggered by the local expression of the auxin transporter AUX1 Parameter der Seitenwurzellänge und -anzahl konnte gezeigt that increases the flux of basipetally translocated auxin into elon- werden, dass lokales Ammoniumangebot die Seitenwurzelbil- gating lateral roots (Giehl et al. 2011, Plant Cell). dung stimuliert, wogegen lokales Nitratangebot v. a. die Sei- These new insights into the regulation of lateral root tenwurzellänge erhöht. Damit wirken Ammonium und Nitrat development will also be used to improve the understanding komplementär auf die Seitenwurzelentwicklung (Lima et al. and manipulation of adventitious root formation in Petunia. 2010, Plant Cell). Im Fall eines lokal begrenzten Fe-Angebots In Petunia shoot cuttings, nutrient supply or dark treatments erhöhen Seitenwurzeln wiederum ihr Längenwachstum. Diese can alter systemic signaling or source-sink relations, which are Anpassung beruht auf einer lokalen Expression des Auxintrans- relevant for adventitious root formation and may provide an porters AUX1, der basipetal transportiertes Auxin in die sich added value in commercial horticulture (Breuillin et al. 2010, streckenden Seitenwurzeln leitet (Giehl et al. 2011, Plant Cell). Plant J.; Klopotek et al. 2010, J. Plant Physiol.). Die dabei gewonnenen Erkenntnisse tragen auch zu einem besseren Verständnis der Adventivwurzelbildung bei Petunien bei. Biosynthesis and functions of secondary metabolites. Within Gezieltes Nährstoffangebot und Dunkelbehandlungen stärken the frame of the EU project ATHENA the Applied Biochemistry die Adventivwurzelbildung der Stecklinge über eine veränderte group has continued phytochemical research directed towards systemische Signaltransduktion bzw. Source-Sink-Beziehungen beneficial health effects of flavonoids as part of the human diet. (Breuillin et al. 2010, Plant J.; Klopotek et al. 2010, J. Plant Phy- Previous experiments using animal model systems have shown siol.). Solche Behandlungen können somit zu einer erweiterten protective effects of an anthocyanin-enriched diet. On-going Wertschöpfung im kommerziellen Gartenbau beitragen. research aims at the phytochemical characterisation of novel plant material as well as the preparative isolation of selected Untersuchungen zur Biosynthese und Funktion von Se- anthocyanins for further medicinal studies in cooperation with kundärmetaboliten. In der Arbeitsgruppe Angewandte the project partners. Biochemie wurden die Arbeiten zur Aufklärung der gesund A candidate gene involved in the control of coumarin accumu- heitsfördernden Wirkung von Flavonoiden in der menschli- lation in Arabidopsis roots has been further characterised. Using chen Ernährung im Rahmen des EU-Projekts ATHENA fortge- fluorescence microscopy, a tissue-specific accumulation of cou- setzt. Vorangehende Arbeiten hatten in Tierversuchen gezeigt, marins could be demonstrated. For a deeper understanding of dass Anthocyan-angereichertes Futter gesundheitsfördernd the biosynthesis of coumarins and related control mechanisms wirkt. Im Mittelpunkt der derzeitigen Versuche stehen die phy- within the context of the general phenylpropanoid metabo- tochemische Charakterisierung von Pflanzenmaterial und die lism, tissue-specific gene expression studies are performed. präparative Aufreinigung strukturell definierter Anthocyane für weiterführende medizinische Studien in Zusammenarbeit Mechanisms of salt tolerance in barley. Mapping populations mit den Projektpartnern. with contrasting salt tolerance of their parental lines have been Ein Kandidatengen, das in die Regulation der Coumarin- studied. By using proteome approaches (total root proteome, Akkumulation in Arabidopsis-Wurzeln eingreift, wurde weiter plasma membrane fractions) a number of candidates has charakterisiert. Mit Hilfe der Fluoreszenzmikroskopie wurde been identified, which are functionally characterised. Further eine gewebespezifische Akkumulation der Coumarine nach- candidates were obtained by complementing a salt-sensitive gewiesen. Für das weitergehende Verständnis der Biosyn- yeast strain with a cDNA library prepared from the salt-tolerant these der Coumarine im Kontext des allgemeinen Phenyl parental line Morex. propanstoffwechsels werden derzeit gewebespezifische Un- Functional analysis includes the study of related Arabidopsis tersuchungen durchgeführt. knock-out mutants. In barley, the expression of candidate proteins will be studied at high temporal and spatial resolution. Untersuchungen zur Salztoleranz in Gerste. In Kreuzungspo- A further aspect will be the analysis of phytohormone profiles pulationen von Gerste, deren Elternlinien eine unterschiedliche and changes of their patterns in response to salt stress in Salztoleranz aufweisen, konnten wir durch Proteomanalysen contrasting genotypes.

126 (Gesamtproteom, Plasmamembran-Fraktionen) eine Reihe von Extension and improvement of the transformation platform. Kandidatengenen identifizieren, welche funktionell näher un- The transformation platform for cereals, which is already well tersucht werden. Weitere Kandidaten wurden durch Komple- established in the Plant Reproductive Biology group, has mentation eines salzsensitiven Hefestamms mit cDNA aus Wur- been extended by efficient and reproducible methods for the zeln einer salztoleranteren Elternlinie (Morex) gewonnen. Mit Poaceae model species Brachypodium distachyon as well as for der funktionellen Charakterisierung von Arabidopsis Knock-out- Boechera polyantha, which is a dicotyledonous experimental Mutanten wurde begonnen. Weitere Arbeiten in ausgewählten model for apomixis research. Both methods represent true Gerstenlinien mit kontrastierender Salztoleranz umfassen die innovations and are being already employed for the functional gewebespezifische Expression der Kandidatengene, die Analy- validation of various candidate genes. In a joint project se von Gen-Expressionsmustern sowie Änderungen in Phyto- conducted along with two groups of the Cytogenetics and hormonprofilen unter Salzstress. Genome Analysis department the Plant Reproductive Biology group has also been successful in a first crucial step towards Erweiterung der Transformationsplattform. Die für Getrei- the establishment of minichromosomes. More specifically, dearten bereits seit einigen Jahren außerordentlich leistungs- chromosomal truncations were achieved in Arabidopsis and fähige Transformationsplattform der Arbeitsgruppe Pflanzliche barley by Agrobacterium-mediated integration of telomer Reproduktionsbiologie wurde durch die Etablierung effizienter sequences. Minichromosomes are regarded as viable and und reproduzierbarer Methoden für das Poaceen-Modell Bra- advantageous plant vectors for the development of a future chypodium distachyon sowie für Boechera polyantha, eine zwei- generation of genetically engineered crops. keimblättrige Modellart für die Apomixis-Forschung, erweitert. Beide Methoden stellen Innovationen dar und werden bereits Characterisation of the initial mechanisms of pollen em- für die funktionelle Validierung diverser Kandidatengene ein- bryogenesis in barley. The unique potential of pollen embryo- gesetzt. Darüber hinaus ist in einem abteilungsübergreifenden genesis for basic research, biotechnology and commercial crop Ansatz durch Agrobakterien-vermittelte Integration von Telo- improvement is in strong contrast to the poor understanding mersequenzen und daraus folgende gezielte Verkürzung von of its underlying biological processes. To understand the initial Chromosomen bei Arabidopsis und Gerste ein bedeutender mechanisms of pollen embryogenesis, a comparative cell bio- Fortschritt hin zur Entwicklung von Minichromosomen gelun- logical study has been conducted to identify structural markers gen, die als praktikable pflanzliche Vektoren die Grundlage ei- and features of the embryogenic and gametophytic pathway. ner zukünftigen Generation gentechnisch verbesserter Kultur- The use of a transgenic barley line with GFP expression in the pflanzen darstellen könnten. nucleus and the development of a custom-made chamber for live-cell imaging allowed for the first time the monitoring of Aufklärung struktureller Mechanismen der Pollenembryo- pollen embryogenesis from the stage of vacuolated immature genese in Gerste. Trotz des enormen Potenzials der Pollen- uni-nucleate barley pollen to the development of multicellular embryogenese für die Grundlagenforschung, die Biotech structures at high temporal resolution. Nine different develop- nologie und kommerzielle Aspekte der Ertragssteigerung bei mental types were described for the highly heterogenic pollen Nutzpflanzen sind deren grundlegende Mechanismen- weit culture, with two types undergoing pollen embryogenesis (Fig. gehend unbekannt. Zum besseren Verständnis der initialen 46, p. 136). The first mitosis either proceeded symmetrically or Mechanismen der Pollenembryogenese hat die Arbeitsgruppe asymmetrically. Ultrastructural and live-cell imaging showed Strukturelle Zellbiologie im Rahmen des Verbundvorhabens that nuclear fusion, which is the crucial mechanism of genome GABI-POEM vergleichende zellbiologische Untersuchungen doubling during barley pollen embryogenesis, can occur at der gametophytischen und embryogenen Pollenentwicklung any developmental stage (Fig. 47, p. 137). This new achieve- durchgeführt. Die Verwendung transgener Gerstenlinien mit ment on the initial mechanisms of pollen embryogenesis in einer GFP-Expression im Zellkern und die Entwicklung einer barley as well as on the optimisation and development of new speziellen Probenkammer für das Live Cell Imaging ermöglich- cell biological tools may stimulate the knowledge-based estab- ten zum ersten Mal die Visualisierung der gesamten Entwick- lishment of double haploids in recalcitrant genotypes or other lung des Pollens während der Pollenembryogenese in hoher economically important crop plants and will eventually allow zeitlicher Auflösung. Dabei unterliefen zwei der neun beobach- the identification of key genes in this process. In addition, the teten Entwicklungstypen in heterogenen Pollenkulturen Polle- newly established methods of pollen fixation and live-cell im- nembryogenese (Fig. 46, S. 136). Es wurde beobachtet, dass aging will also be useful to study other developmental proc- die erste Mitose sowohl symmetrisch als auch asymmetrisch esses in plants. erfolgen kann. Die Ergebnisse der Ultrastrukturanalysen und des Live Cell Imaging zeigten, dass es sich bei der Kernfusion, The Yeast Genetics group succeeded in the system-biological die zu jedem Zeitpunkt der Entwicklung stattfinden kann (Fig. modelling of the tannic acid degradation pathway in the 47, S. 137), um den entscheidenden Mechanismus zur Genom- non-conventional yeast Arxula adeninivorans. The completely verdopplung während der Pollenembryogenese handelt. Die- sequenced and annotated genome of this yeast in combination se umfangreichen neuen Kenntnisse zur Initiation der Pollen- with genetic and biotechnical tools (selection of respective embryogenese in Gerste und die Optimierung bzw. Entwick- mutants, fusion products, mitotic segregants and transformants) lung neuer zellbiologischer Methoden könnten zur Etablierung allowed the identification and characterisation of all enzymes

127 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

Doppelhaploider in weiteren Genotypen als auch in wirtschaft- (including the respective genes) of this important pathway. So lich relevanten Kulturpflanzen führen und die Identifizierung far, Arxula is the first yeast containing two secretory tannases beteiligter Gene ermöglichen. Darüber hinaus sind die neu with different substrate spectra. It is able to digest condensed entwickelten bzw. optimierten zellbiologischen Methoden in and hydrolysable tannic acids via gallic acid, pyrogallol and vielen Bereichen der Pflanzenforschung anwendbar. 2-hydroxy mucoic acid to succinate. All genes involved in this pathway are inducible by tannic acid and gallic acid. Mit der systembiologischen Modellierung des Abbauweges von Tanninen gelang der Arbeitsgruppe Hefegenetik am Bei- In the biosensor laboratory of the Yeast Genetics group, new spiel der Hefe Arxula adeninivorans die Aufklärung eines bisher biosensors have been developed and validated for the fast and noch unbekannten eukaryotischen Stoffwechselweges. Das reproducible detection of arbuscular mycorrhiza fungi (AMF) komplett am IPK sequenzierte und annotierte Genom dieser and of phytopathogenic viruses on plants as well as of hormonal Hefe in Kombination mit genetischen und gentechnischen activities and pharmaceuticals in environmental samples and Tools (Herstellung entsprechender Mutanten, Fusionsprodukte, animals. Based on DNA-DNA or DNA-RNA hybridisations high- mitotischer Segreganten und Transformanten) erlaubte sowohl throughput microtiter plate assays and SPR chips have been die Identifizierung als auchC harakterisierung aller Enzyme (ein- established for analyses of mycorrhizal-plant root interactions schließlich deren Gene) dieses für die Grundlagenforschung or the detection of phytopathogenic RNA viruses in wheat and und die biotechnologische Forschung äußerst bedeutenden potato. Stoffwechsels. So istArxula die bisher einzig bekannte Hefe, die zwei Tannasen mit unterschiedlichen Substratspektren sezer- Establishment of methods for metabolic flux analysis in niert und damit sowohl kondensierte als auch hydrolysierbare plant seeds. In the Systems Biology group, a method for de- Tannine über Gallussäure, Pyrogallol und 2-Hydroxymukonsäu- termining intracellular reaction rates in central metabolism has re zu Succinat abbauen kann. Bei all diesen Genen handelt es been implemented, adapted to legume and cereal seeds, and sich um Tannin- bzw. Gallussäure-induzierbare Gene. optimised and partly automated to yield a competitive throughput. This method, named Metabolic Flux Analysis (MFA), allows Im Biosensorlabor der Arbeitsgruppe Hefegenetik wurden the generation of highly detailed flux maps which include neue Biosensoren entwickelt und validiert, mit denen sich ar- cyclic, parallel, and bidirectional fluxes. As metabolic fluxes buskuläre Mykorrhizapilze und phytopathogene Viren an Pflan- cannot be measured directly, the method relies on simulations zen einerseits sowie Hormonaktivitäten und Pharmazeutika in of feeding experiments with stable isotopes, which are then Umweltproben und Tieren andererseits schnell, eindeutig und compared to respective laboratory experiments. By adjusting reproduzierbar nachweisen lassen. So gelang die Etablierung the parameters of the model, simulation and experiment are von auf Mikrotiterplatten-Assays bzw. auf der SPR-Plattform brought closer together in an iterative manner. Setting up the beruhenden Hochdurchsatzverfahren zur Analyse der Interak- method requires expertise in biochemistry, mathematics, and tion von arbuskulären Mykorrhizapilzen mit Wurzeln bzw. zum computer science. MFA is currently being applied in several Nachweis von phytopathogenen RNA-Viren an Weizen und BMBF-funded projects. Kartoffelpflanzen.

Etablierung von Methoden zur Analyse von Stoffwechsel- Nicolaus von Wirén, November 2011 flüssen. In der Arbeitsgruppe Systembiologie wurde eine Me- thode zur Bestimmung von intrazellulären Reaktionsraten im zentralen Stoffwechsel von Leguminosen- und Getreidesamen aufgesetzt. Diese Methode der metabolischen Stoffflussanalyse (MFA) ermöglicht die Erstellung von sehr detaillierten Flusskar- ten, die auch zyklische, parallele, und bidirektionale Flüsse be- inhalten. Da Stoffflüsse nicht direkt gemessen werden können, werden in dieser Methode Markierungsexperimente simuliert, die mit entsprechenden Laborexperimenten verglichen werden. Die Parameter in den Modellen werden dann in einem iterativen Prozess angepasst, um Experiment und Simulation ähnlicher werden zu lassen. Die Etablierung der Methode setzt profunde interdisziplinäre Kenntnisse in Biochemie, Mathema- tik und Informatik voraus. MFA wird momentan in verschiedenen Drittmittelprojekten angewendet.

Nicolaus von Wirén, November 2011

128 Research Group: Molecular Plant Nutrition Head: Prof. Nicolaus von Wirén

Scientists Goals

IPK financed Characterisation of morphological and physiological responses Ahkami, Amirhossein (0.50, till 31.03.2010) of plants subjected to biotic and abiotic stress conditions with Bauer, Bernhard (0.50) a strong focus on changing nutrient supplies, and identification Bohner, Anne (0.50 / 1.00) of rate-limiting steps in carbohydrate and nutrient metabolism Gruber, Benjamin D., Dr. of stressed plants. Hajirezaei, Mohammad R., Dr. Hettwer Giehl, Ricardo Fabiano (0.50/1.00, since 01.04.2011) Weishaar, Claudia (0.50, till 30.09.2011) Research Report

Grant Positions Nutrient sensing. Changes in the architecture of lateral roots Bohner, Anne (0.50 BMBF, 01.08.-30.09.2011) in response to a localised supply of nutrients are regarded Boylu, Baris (0.50 BMBF, since 01.09.2011) as a morphological read-out for nutrient sensing. We used Donath, Sebastian (0.50 DFG) horizontally- or vertically-split agar plates with localised Eggert, Kai, Dr. (BMBF, since 01.08.2011) nitrogen supplies to describe lateral root growth into Eroglu, Seckin (0.50 BMBF, since 08.10.2010) nitrogen-containing agar patches. We observed that localised Ghaffari, Mohammad Reza (0.50 BMBF) ammonium supply strongly stimulated lateral root branching Hosseini, Seyed Abdollah (0.50 BMBF, since 20.06.2011) in Arabidopsis, leading to a phenotype with highly branched, Kim, Young-Min (0.50 DFG, till 31.07.2010) higher-order lateral roots, while localised nitrate supply mainly Köber, Julia Verena (0.50 DFG, 01.04.2010-31.03.2011) enhanced lateral root elongation. Thus, ammonium and Lingam, Brahmasivasenkar (0.50 Pakt für Forschung und nitrate act in a complementary manner to shape lateral root Innovation, since 01.06.2011) development. An analysis of Arabidopsis mutants defective Manasse Laginha, Alberto (0.50 DFG, since 01.01.2011) in single or multiple AMT-type ammonium transporters then Schmid, Nicole (0.50 BMBF) revealed that AMT1;3 had a particularly strong impact on Shi, Rongli, Dr. (0.75 DFG, since 17.11.2010) lateral root initiation. 15N-labeled ammonium uptake studies Ye, Fanghua (0.50 IZN, since 05.05.2010) indicated that ammonium-triggered lateral root branching was not a nutritional response. Thus AMT1;3, which is expressed in Visiting Scientists/Scholars the rhizodermis and cortex, acts upstream of an ammonium Ahkami, Amirhossein (University Hohenheim, 01.04.-30.09.2010) sensing event that stimulates the initiation of lateral roots Alvarez, Maria (BMBF, 11.09.-21.09.2010) (Lima et al. 2010, Plant Cell). Barunawati, Nunun (Indonesian government) We have meanwhile extended our approach to other nutrients Boylu, Baris (self-financed, 26.08.-31.08.2011) and observed that lateral root development is not a common Carillo, Nestor, Prof. (DFG, 09.09.-23.09.2010; IPK, 12.05.-17.05.2011) response to localised nutrient supplies. When we conducted Duan, Fengying (Chinese government, since 01.11.2010) an in-depth analysis for lateral root responses to localised Fe Fedoseyenko, Dmitriy (self-financed, 01.01.-31.12.2010; supply, a local upregulation of the auxin transporter AUX1 was 01.07.-31.07.2011) found to be a prerequisite for a local auxin accumulation in lat- Ghaboli, Medhi (Ministry of Science, Research and Technology eral root apices (Fig. 44, p. 130). This auxin mainly derives from Iran, since 05.08.2011) the rootward auxin stream and promotes the elongation of lat- Ghorbani Javid, Majid (Ministry of Science, Research and eral roots (Giehl et al. 2011, Plant Cell). Currently, we search for Technology Iran, 11.03.-10.09.2010) Arabidopsis mutants affected in targeted lateral root responses Hettwer Giehl, Ricardo Fabiano (CAPES, 03.05.2010-31.03.2011) and investigate root developmental processes, in which nutri- Isik, Zeynep (Fellowship of University Hohenheim, 18.03.- ents alter phytohormone signalling pathways (Dr. B. Gruber, 26.03.2010) Dr. Ricardo Giehl). Kim, Young-Min (self-financed, since 01.02.2011) Monteoliva, Mariela (BMBF, 14.11.-20.12.2010) To establish a screening platform for the selection of barley Schmidt, Susanne (Humboldt fellowship, 14.05.-30.06.2011) genotypes with rapid root growth under field conditions, we Takahashi, Michiko, Prof. Dr. (self-financed, 22.04.-21.09.2011) have developed a fertilisation device for the deep placement Tognetti, Vanessa, Dr. (IPK, 12.05.-17.05.2011) of fertiliser bands in different depths. These bands provide Yuan, Linxing, Prof. (Robert Bosch Foundation, 12.08.-02.09.2011) nutrients that are otherwise limiting for plants growing on these Zurbriggen, Mathias, Dr. (DFG, 21.06.-30.06.2010; 29.08.- plots. As soon as the roots of individual plants reach a fertiliser 23.09.2010) band, they regreen and change light-absorbing properties.

129 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

Fig. 44 The regulation of lateral root development by localised availability of Fe. (A) Root architecture of Arabidopsis thaliana plants in response to Fe supply. Seedlings were grown on half-strength MS medium without Fe for 7 days before transferring to segmented agar plates containing half-strength MS and 75 µM ferrozine. Fe(III)-EDTA (µM) was added at the indicated concentrations to all three segments (homogeneous supply) or only to the middle segment (localised supply). Plants were scanned after 15 days of growth on Fe treatments and representative plants are shown. Horizontal lines represent the borders between the three segments. (B) Model for the regulation of lateral root development under localised availability of Fe. Uptake of Fe via the high-affinity 2+Fe transporter IRT1 increases the symplastic root Fe pool, which can be replenished by Fe derived from the shoot or other parts of the root. The local enrichment in symplastic root Fe leads to an upregulation of the auxin importer AUX1 channelling auxin from the rootward auxin stream towards the lateral root tip. This response is re-inforced by the integration of systemic Fe deficiency signals originated from other parts of the root. Increased rootward auxin movement in lateral roots triggers the longitudinal elongation of mature cells, subsequently resulting in enhanced lateral root elongation.

A regular recording of hyperspectral data from these plants In our role as coordinator of a DFG research group on nitrogen allows us to detect when genotypes reach a fertiliser band and remobilisation in senescing plants, we focused on the role of to set up a ranking of lines according to their root penetration ammonium and urea transport in senescing leaves as in Arabi- rate (B. Baylu, B. Bauer). dopsis the ammonium and urea transporter genes AMT1;1 and DUR3 are upregulated during senescence. We have now found Plant growth-promoting rhizobacteria. In an EU-funded that DUR3 mainly serves for urea retrieval from the leaf apo- cooperation a Raoultella terrigena strain had been isolated from plast, thereby safeguarding urea-N that may otherwise be lost. the rhizosphere of wheat and found to promote plant growth With the successful establishment of an isotope ratio mass spec- in field studies. Since the reproducibility of yield increases by trometry, retranslocation studies with 15N-labeled substrates inoculating this or other plant growth-promoting bacteria is have been undertaken in transgenic Arabidopsis and barley still variable, we established an Arabidopsis growth assay with plants with deregulated expression of ammonium and urea vertically oriented agar plates, in which Raoultella stimulates transporter genes to determine the retranslocation efficiency root and shoot growth by more than twofold. However, this of different phloem-mobile N forms. Moreover, polyamine growth effect depends on the supplied nitrogen form and on measurements have been undertaken, since this class of puta- the buffer capacity of the medium. Recently, we conducted a tive phytohormones strongly responds to ammonium nutrition transcriptome analysis of inoculated versus non-inoculated and senescence and might have a regulatory impact on the de- roots and shoots and found several genes that show a velopment of leaf senescence (A. Bohner, S. Donath). particularly strong induction or repression after inoculation. After qPCR verification, T-DNA insertion lines for these genes have been isolated and are currently being characterised for their growth response to Raoultella. Within a Leibniz Association- funded PAKT project with the IPB Halle we aim at identifying Arabidopsis genes that determine the plant’s responsiveness to growth-promoting bacteria (S. Lingam).

130 In parallel, field-grown wheat plants that were senescing at Publications different stages were used to collect leaf and spike material for the analysis of N and metal micronutrients. Together with gene Peer Reviewed Papers expression studies and the analysis of metals and potential chelators in senescing leaves, this approach showed that 2010 phytosiderophores and citrate play an important role for Fe Br e u i l l i n , F., J. Sc h r a m m , M. Ha j i r e z a e i , A. Ah k a m i , P. Fa v r e , U. Dr u e g e , mobilisation in senescing source leaves as a prerequisite B. Ha u s e , M. Bu c h e r , T. Kr e t z s c h m a r , E. Bo s s o l i n i , C. Ku h l e m e i e r , for Fe retranslocation to sink organs (R. Shi, N. Barunawati). E. Mar tinoia, P. Fr a n k e n , U. Sc h o l z & D. Re i n h a r d t : Phosphate systemically inhibits development of arbuscular mycorrhiza Iron efficiency strongly relies on the genetically determined in Petunia hybrida and represses genes involved in ability of plants to increase Fe acquisition in the rhizosphere mycorrhizal functioning. Plant J. 64 (2010) 1002-1017. either by Fe(III) reduction and Fe2+ uptake, as in strategy I El-Ba n n a , A., M.R. Ha j i r e z a e i , J. Wi s s i n g , Z. Al i , L. Va a s , E. H e i n e -Do b b e r n a c k , plants, or by the release of phytosiderophores and subsequent H.J. Ja c o b s e n , H.M. Sc h u m a c h e r & H. Ki e s e c k e r : Over-expression of Fe(III)-phytosiderophore uptake, as in graminaceous (strategy PR-10a leads to increased salt and osmotic tolerance in potato II) plants. To identify genes controlling Fe acquisition in Arabi- cell cultures. J. Biotechnol. 150 (2010) 277-287. dopsis, a collection of 7,000 Arabidopsis T-DNA insertion lines Gr a f f , L., P. Ob r d l i k , L. Yu a n , D. Lo q u e , W.B. Fr o m m e r & N. v o n has been screened on a self-made calcareous substrate for phe- Wi r é n : N-terminal cysteines affect oligomer stability of the notypes that show less or more severe Fe deficiency-induced allosterically regulated ammonium transporter LeAMT1;1. J. chlorosis than wild type plants. This approach led to the iden- Exp. Bot. 62 (2010) 1361-1373. tification of two lines that suffer from Fe deficiency-induced Kl o p o t e k , Y., K.T. Ha e n s c h , B. Ha u s e , M.R. Ha j i r e z a e i & U. Dr u e g e : Dark chlorosis more than wild-type plants and are defective either exposure of petunia cuttings strongly improves adventitious in a gene involved in the phenylpropanoid pathway or another root formation and enhances carbohydrate availability during gene encoding a MYB-type transcription factor. In the frame of rooting in the light. J. Plant Physiol. 167 (2010) 547-554. a Plant-KBBE project, these genes will be further characterised Li m a , J.E., S. Ko j i m a , H. Ta k a h a s h i & N. v o n Wi r é n : Ammonium triggers for their role in Fe efficiency using reverse genetic approaches lateral root branching in Arabidopsis in an AMMONIUM (N. Schmid, S. Eroglu). TRANSPORTER1;3-dependent manner. Plant Cell 22 (2010) 3621-3633. Hexokinases are central enzymes in glycolysis that phos- Ri e b e s e e l , E., R.E. Hä u s l e r , R. Ra d c h u k , T. Me i t z e l , M.R. Ha j i r e z a e i , R.J. Ne i l phorylate glucose and related hexoses and thereby can fulfil Em e r y , H. Kü s t e r , A. Nu n e s -Ne s i , A.R. Fe r n i e , W. We s c h k e & H. We b e r : sensing functions in carbohydrate metabolism. In tobacco, six The 2-oxoglutarate/malate translocator mediates amino acid hexokinase isoforms have been identified and silenced in trans- and storage protein biosynthesis in pea embryos. Plant J. 61 genic plants. Only RNAi lines of NtHXK1 showed chlorosis, se- (2010) 350-363. verely damaged mesophyll tissue and an excess accumulation Zu r b r i g g e n , M., N. Ca r r i l l o & M. Ha j i r e z a e i : Engineering the future. of starch. Metabolite analyses and functional studies indicated Development of transgenic plants with enhanced tolerance to that the loss of activity of this cytosolic hexokinase blocked adverse environments. Biotechnol. Genet. Eng. Rev. 27 (2010) downstream processing of glucose and causing starch accu- 33-56. mulation in chloroplasts. This study indicates a central function Zu r b r i g g e n , M.D., N. Ca r r i l l o & M.R. Ha j i r e z a e i : ROS signaling in the of NtHXK1 in primary carbon metabolism that can be primarily hypersensitive response: When, where and what for? Plant explained by its catalytic activity (Y.-M. Kim, M. Hajirezaei). Signal. Behav. 5 (2010) 393-396.

The expression of a bacterial flavodoxin in Arabidopsis, tobacco, potato or barley improves plant tolerance to biotic and abiotic stresses. To circumvent the complex phenotypes resulting from stress exposure, we generated transgenic tobacco lines, in which ferredoxin is knocked-down by RNA interference. Silenced lines exhibited arrested growth, diffuse or variegated leaf chlorosis and inhibited photosynthesis. Chloroplast or nuclear transformation of these plants with a cyanobacterial flavodoxin gene restored photosynthetic activity and the wild- type phenotype. In addition, these transgenics were more tolerant than wild-type plants to oxidative stress imposed by the redox-cycling herbicide methyl viologen. These results show that cyanobacterial flavodoxin is able to functionally replace plant-endogenous ferredoxin and suggest that the superior stability of flavodoxin and its more efficient cycling of reduction equivalents enhance stress tolerance (Blanco et al. 2011, Plant J.).

131 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

2011 PhD and Diploma Thesis Ba z a r g a n i , M.M., E. Sa r h a d i , A.A. Bu s h e h r i , A. Ma t r o s , H.P. Mo c k , 2010 M.R. Na g h a v i , V. Ha j i h o s e i n i , M. Ma r d i , M.R. Ha j i r e z a e i , F. Mo r a d i , Ah k a m i , A.: Molecular physiology of Adventitious Root Forma- B. Eh d a i e & G.H. Sa l e k d e h : A proteomics view on the role of tion (ARF) in Petunia hybrida cuttings. (PhD Thesis) Martin- drought-induced senescence and oxidative stress defense Luther-Universität Halle-Wittenberg, Naturwissenschaftli- in enhanced stem reserves remobilization in wheat. J. che Fakultät I, Halle/S. (2010) 150 pp. Proteomics 74 (2011) 1959-1973. Bl a n c o , N.E., R.D. Ce c c o l i , M.E. Se g r e t i n , H.O. Po l i , I. Vo s s , M. Me l z e r , 2011 F.F. Br a v o -Al m o n a c i d , R. Sc h e i b e , M.R. Ha j i r e z a e i & N. Ca r r i l l o : Bo h n e r , A.: Membrane transport and long-distance translocation Cyanobacterial flavodoxin complements ferredoxin of urea in Arabidopsis thaliana. (PhD Thesis) Universität deficiency in knocked-down transgenic tobacco plants. Hohenheim, Stuttgart (2011) 109 pp. Plant J. 65 (2011) 922-935. Gi e h l , R.F.H.: Identification of regulatory factors determining Ca r v a l h a i s , L.C., P.G. De n n i s , D. Fe d o s e y e n k o , M.R. Ha j i r e z a e i , R. Bo r r i s s nutrient acquisition in Arabidopsis. (PhD Thesis) Universität & N. v o n Wi r é n : Root exudation of sugars, amino acids, and Hohenheim, Stuttgart (2011) 157 pp. organic acids by maize as affected by nitrogen, phosphorus, Lu t z , U.: The role of the transcription factor ANAC020 in iron potassium, and iron deficiency. J. Plant Nutr. Soil Sc. 174 nutrition and flowering time regulation in Arabidopsis (2011) 3-11. thaliana. (Diploma Thesis) Universität Hohenheim, Stuttgart d e Bo n a , F., D. Fe d o s e y e n k o , N. v o n Wi r é n & F.A. Mo n t e i r o : Nitrogen (2011) 90 pp. utilization by sulfur-deficient barley plants depends on the Vo r r a t h , I.: Genotypische Unterschiede beim Einfluss der N- nitrogen form. Environ. Exp. Bot. 74 (2011) 237-244. Form auf die Metabolitzusammensetzung in Weizen. (Mas- De b a s t , S., A. Nu n e s -Ne s i , M.R. Ha j i r e z a e i , J. Ho f m a n n , U. So n n e w a l d , ter Thesis) Hochschule Anhalt, Bernburg (2011) 45 pp. A.R. Fe r n i e & F. Bö r n k e : Altering trehalose-6-phosphate We i s h a a r , C.: Impact of the plant growth-promoting rhizobac- content in transgenic potato tubers affects tuber growth terium Raoutella terrigena TFi08N on plant growth and root and alters responsiveness to hormones during sprouting. architecture. (PhD Thesis) Martin-Luther-Universität Halle- Plant Physiol. 156 (2011) 1754-1771. Wittenberg, Halle / S. (2011) 97 pp. Gr a f f , L., P. Ob r d l i k , L. Yu a n , D. Lo q u é , W.B. Fr o m m e r & N. v o n Wi r é n : N-terminal cysteines affect oligomer stability of the allosterically regulated ammonium transporter LeAMT1;1. J. Exp. Additional Publications 2009 Bot. 62 (2011) 1361-1373. Ba u e r , B. & N. v o n Wi r é n : Ansätze aus der Pflanzenernährung Kö s t e r , J., H. Ha y e n , N. v o n Wi r é n & G. We b e r : Isoelectric focusing zur genetischen Anpassung von Kulturpflanzen an den of small non-covalent metal species from plants. Klimawandel. Vortr. Pflanzenzücht. 81 (2009) 63-70. Electrophoresis 32 (2011) 772-781. La n q u a r , V., D. Lo q u e , F. Ho r m a n n , L.X. Yu a n , A. Bo h n e r , W.R. Kö s t e r , J., R. Sh i , N. v o n Wi r é n & G. We b e r : Evaluation of different col- Engelsberger , S. La l o n d e , W.X. Sc h u l z e , N. v o n Wi r é n & W.B. umn types for the hydrophilic interaction chromatographic Fr o m m e r : Feedback inhibition of ammonium uptake by a separation of iron-citrate and copper-histidine species from phospho-dependent allosteric mechanism in Arabidopsis. plants. J. Chromatogr. A 1218 (2011) 4934-4943. Plant Cell 21 (2009) 3610-3622. v o n Wi r é n , N.: Grand challenges in plant nutrition. Front. Plant Sci. 2 (2011) 4; doi:10.3389/fpls.2011.00004.

Other Papers 2010 Ba u e r , B.: Winterweizenanbau – Bestandesaufbau im Frühjahr auf den Sortentyp abstimmen? Innovation 1 (2010) 16-18. Ba u e r , B.: Qualitätsweizen produzieren – An welchen Schrauben ist zu drehen, um auch bei hohen Erträgen sichere Eiweißge- halte zu erzielen? Getreidemagazin 2 (2010) 112-114. Le m k e , R., M. Ha j i r e z a e i , B.H. Ju n k e r , J. Mü l l e r, B. Us a d e l , M. Le p s & F. Sc h r e i b e r : MMM – Mehrskalen-Stoffwechselmodelle zur Systembiologie in Getreiden – ein integrativer Ansatz für die Biomasseforschung. Systembiologie.de 2 (2010) 51-53.

132 Research Group: Applied Biochemistry Head: Dr. Hans-Peter Mock

Scientists Research Report

IPK financed Within the EU ATHENA project the group is responsible for the Döll, Stefanie (0.50, since 01.12.2011) phytochemical characterisation of plant materials. Plant tissues Matros, Andrea, Dr. (till 30.11.2011) are profiled forflavonoids and related phenylpropanoids with Peukert, Manuela (0.50, since 01.12.2011) potentially beneficial health effects by using HPLC-UV-MS. Plant materials studied comprise transgenic tomato fruits with Grant Positions ectopic expression of transcription factors regulating anthocyanin Dittbrenner, Anke (Industry) biosynthesis, maize, blood orange accessions, grape cell cultures Döll, Stefanie (0.50 Industry, till 30.05.2010 and as well as barley and wheat lines. A major goal of the project is 01.03.-30.06.2011; 0.50 BMBF, 31.05.2010-28.02.2011 and to study the effects of individual anthocyanins in feeding studies 01.07.-30.11.2011) using animal model systems. For this purpose, preparative Hedtmann, Christiane (0.50 Industry, 01.04.-30.06.2011; isolation of anthocyanins from different sources has been started 0.50 BMBF, 01.07.-31.12.2011) by adopting HPLC methods to a larger scale (A. Dittbrenner, A. Kaspar, Stephanie (0.50/1.00 BMBF, till 31.03.2011) Matros; collaboration with ATHENA partners). Lippmann, Rico (0.50 BMBF) The biochemical and molecular analysis of exudates biosynthe- Matros, Andrea, Dr. (BMBF, since 01.12.2011) sis in glandular trichomes of tobacco varieties has been con- Merx, Kathleen (0.50 BMBF, till 28.02.2011) tinued. Methods for analysis of diterpenoids and for sucrose Peukert, Manuela (0.50 DFG, till 30.11.2011) esters have been applied to quantify the amounts of individual Tandron, Yudelsy Antonia, Dr. (0.50 BMBF, till 30.06.2010) diterpenoids and of sucrose esters in exudates of about forty tobacco accessions. Next generation sequencing technique Visiting Scientists/Scholars was applied to sequence trichome cDNA libraries generated for Capdesuner Ruiz, Yanelis Karina (DAAD scholarship, three selected accessions. Data are currently used to perform till 29.08.2010; 16.06.-10.12.2011) array experiments with a larger set of accessions (Y. Capdes- Eick, Manuela (self-financed, 18.07.-30.07.2010) uner, Y. Tandron, R. Lippmann, A. Matros). Fila, Jan (EU COST, 02.03.-28.03.2010; self-financed, 14.11.-27.11.2010; DAAD, 31.07.-18.09.2011) The functional characterisation of a putative regulatory factor Hashemi, Amenehsadat (Ministry of Science, Research and of coumarin biosynthesis was continued. Knock-out mutant Technology Iran, 19.06.-18.12.2011) and wild-type plants were grown under conditions favouring Huerta Ocampo, José Àngel (self-financed, 04.04.-30.11.2011) coumarin accumulation and array experiments were performed Janmohammadi, Mohsen (Research scholar Iran, to compare the expression profiles as a means to further char- till 22.04.2010) acterise the regulatory context of the candidate gene. Potential Lattanzio, Giuseppe (self-financed, 01.08.-22.11.2010) involvement of coumarins in iron nutrition is studied in collabo- Mazzucotelli, Elisabeth, Dr. (EU COST, 18.04.-28.05.2010) ration with the group of N. von Wirén (D. Berreth, S. Döll). Witzel, Katja, Dr. (self-financed, 08.11.-26.11.2010; 16.05.-20.05.2011; 01.11.-08.11.2011) The functional characterisation of the stress-induced co-chap- Yadav, Sunita (DAAD fellowship, 01.09.-30.11.2011) erone protein STINT and related STIAT proteins in Arabidopsis has been continued. A major focus of recent research was the potential phosphorylation of these co-chaperones. Methods for the isolation of phosphorylated STINT or STIAT proteins have Goals been introduced. Putative phosphorylation sites have been identified by mass spectrometry (S. Endress, C. Hedtmann). The main research interest of the group is the biosynthesis and regulation of secondary metabolism in plants. Protective A major area of research in the group has been the study of functions against abiotic and biotic stresses but also to the changes in proteome patterns during barley seed develop- potential health effects of these metabolites as a part of the ment. The multiplexed LC-MS analysis of individual barley grain human diet are important aspects. A major goal is to gain tissues isolated by laser capture micro-dissection combined with further insights into regulatory programmes and mechanism pressure catapulting (LMPC), allowed for the identification of of resource allocation into different branches of secondary several proteins showing tissue-specificity in their expression. metabolism. Proteome approaches are applied to study the Results underline the distinct biological functions of the two in- integration of secondary metabolism into overall cellular vestigated tissues within the developing barley grain (S. Kaspar, defence mechanisms. K. Merx, A. Matros; collaboration with W. Weschke). In parallel, an

133 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

Fig. 45 Matrix-assisted laser desorption/ionisation mass spectrometric imaging (MALDI-MSI) analysis of immature barley (Hordeum vulgare) grain tissues. A) Representative scheme of MALDI-MSI approach. First cryo-sections from barley grains are prepared and a suitable matrix is applied onto the tissue section. The data are acquired by rasterising the complete tissue section with a laser-beam and capturing the full mass spectrum at each irradiation point with a MALDI-TOF device. An average mass spectrum is obtained and ion intensity maps are used to infer the distribution of distinct m / z values over the tissue. B) Examples from analyses of 30 μm sections of 3-d after pollination (DAP) developing barley grains are shown. The multi-ion image shows molecular masses m/z 773.4 (mangenta) and m / z 871.5 (turquoise) from a longitudinal section (left). Also corresponding selected-ion images and the respective average mass spectra from cross sections are shown (centre). Identification of underlying molecular compounds was based on MS fragmentation analysis directly from the cryo-sections.

MALDI-MS imaging platform able to profile developing barley brane proteome of plants from both parental lines grown under grains has been established. The protein compositions of grains salt stress. The proteome of the plasma membrane fraction was sampled shortly after fertilisation have displayed either tissue- analysed by multiplexed LC-MS of peptides resulting from diges- specific or co-localised protein expression patterns. Currently, tion with trypsin; complementary datasets were obtained by LMPC and on-tissue digestion are being used to determine the using elastase for protein digestion. The full root proteome ap- identity of the underlying proteins. In addition, funding by the proach was performed in a kinetic manner for the parental lines DFG has been provided for the development of MALDI-MS im- of the Steptoe-Morex population. Several candidates associated aging of metabolites in developing barley grains (see Fig. 45). with the higher tolerance of the Morex cultivar were found in Protocols for detecting small molecules in cryo-dissected im- more than one of the studies, including the earlier data from the mature barley grains have been introduced and were success- complementation of a salt-sensitive Saccharomyces strain. Func- fully transferred to tobacco root tissue (M. Peukert, A. Matros). tional characterisation of candidates has been continued using The resulting data set revealed reproducible metabolite distri- different approaches, such as analysis ofArabidopsis KO lines and butions specific to various tissues as well to different develop- targeted studies in yeast and barley. A particular set of candi- mental stages of barley seeds. A number of these signals were dates point to the significance of plasma membrane proteome tentatively identified as being derived from oligosaccharides as well as lipid composition and to the involvement of control by and phospholipids (M. Peukert, A. Matros; collaboration with W. specific phytohormones. Weschke). A novel project aims at elucidating the potential of Euphorbia The project on salt stress responses in barley mapping popu- lathyris for generation of biofuels. This plant grows on marginal lations with contrasting tolerances has been continued (K. Wit- soils thereby not competing with crops and is characterised by zel, A. Matros; collaboration with A. Börner and all groups of the the accumulation of energy-rich triterpenoids (EULAFUEL; R. Physiology and Cell Biology department). Novel candidates as- Lippmann). sociated with the higher salt tolerance of the Morex variety have been revealed by comparative analysis of the root plasma mem-

134 Publications Books and Book Chapters

Peer Reviewed Papers 2011 Ma t r o s , A., S. Ka s p a r , S. Te n z e r , M. Ki p p i n g , U. Se i f f e r t & H.P. Mo c k : 2010 Label-free liquid chromatography-based quantitative Ag a r w a l , R., A. Ma t r o s , M. Me l z e r , H.P. Mo c k & J.K. Sainis: proteomics: challenges and recent developments. In: Heterogeneity in thylakoid membrane proteome of Ra n c o u r t , G.C. (Ed.): Proteomics: Methods, applications and Synechocystis 6803. J. Proteomics 73 (2010) 976-991. limitations (Protein biochemistry, synthesis, structure and Ka s p a r , S., A. Ma t r o s & H.P. Mo c k : Proteome and flavonoid analysis cellular functions). Nova Science Publishers Inc., New York reveals distinct responses of epidermal tissue and whole (2011) 103-136. leaves upon UV-B radiation of barley (Hordeum vulgare L.) Mø l l e r, A.L.B., K. Wi t z e l , A. Ve r t o m m e n , V. Ba r k h o l t , B. Sv e n n s o n , S. seedlings. J. Proteome Res. 9 (2010) 2402-2411. Ca r p e n t i e r , H.-P. Mo c k & C. Fi n n i e : Plant plasma membrane Ka s p a r , S., D. We i e r , W. We s c h k e , H.P. Mo c k & A. Ma t r o s : Protein proteomics: challenges and possibilities. In: Iv a n o v , A.R. & analysis of laser capture micro-dissected tissues revealed A.V. La z a r e v (Eds.): Sample preparation in biological mass cell-type specific biological functions in developing barley spectrometry. Springer, Dordrecht etc. (2011) 411-434. grains. Anal. Bioanal. Chem. 398 (2010) 2883-2893. Sreenivasulu , N., L. Bo r i s j u k , B.H. Ju n k e r , H.P. Mo c k , H. Ro l l e t s c h e k , U. Se i f f e r t , W. We s c h k e & U. Wo b u s : Barley grain development toward Other Papers an integrative view. Int. Rev. Cell Mol. Biol. 281 (2010) 49-89. 2010 Wi t z e l , K., A. We i d n e r , G.K. Su r a b h i , R.K. Va r s h n e y , G. Ku n z e , G.H. Bu c k - Se i f f e r t , U., F. Bo l l e n b e c k , H.P. Mo c k & A. Ma t r o s : Clustering of So r l i n , A. Bö r n e r & H.P. Mo c k : Comparative analysis of the grain crop phenotypes by means of hyperspectral signatures proteome fraction in barley genotypes with contrasting using artificial neural networks. Proceedings of the 2nd IEEE salinity tolerance during germination. Plant Cell Environ. 33 Workshop on Hyperspectral Imaging and Signal Processing: (2010) 211-222. Evolution in Remote Sensing WHISPERS 2010, Reykjavik/ Iceland, IEEE Press (2010) 31-34. 2011 Ba z a r g a n i , M.M., E. Sa r h a d i , A.A. Bu s h e h r i , A. Ma t r o s , H.P. Mo c k , M.R. Na g h a v i , V. Ha j i h o s e i n i , M. Ma r d i , M.R. Ha j i r e z a e i , F. Mo r a d i , PhD and Diploma Theses B. Eh d a i e & G.H. Sa l e k d e h : A proteomics view on the role of 2010 drought-induced senescence and oxidative stress defense d e l Ro c i o Mo y a Ru i z , M.: Expression differencial de proteinas in enhanced stem reserves remobilization in wheat. J. Pro- radiculares del trigo (Triticum aestivum L.) a la adhesion de teomics 74 (2011) 1959-1973. Azospirillum brasiliense Cd. (Master Thesis) La Paz/Mexico Ka s p a r , S., M. Pe u k e r t , A. Sv a t o s , A. Ma t r o s & H.P. Mo c k : MALDI- (2010) 105 pp. imaging mass spectrometry – An emerging technique in plant biology. Proteomics 11 (2011) 1840-1850. 2011 Ma t r o s , A., S. Ka s p a r , K. Wi t z e l & H.P. Mo c k : Recent progress in liquid Be r r e t h , D.-C.: Analyse der gewebespezifischen Expression von chromatography-based separation and label-free quantitative Genen der Stressabwehr in Arabidopsis-Wurzeln mit beson- plant proteomics. Phytochemistry 72 (2011) 963-974. derer Berücksichtigung von Genen des Phenylpropan- Wi t z e l , K., C. Pi e t s c h , M. St r i c k e r t , A. Ma t r o s , M. Rö d e r , W. We s c h k e , stoffwechsels. (MasterT hesis) Fakultät Naturwissenschaften, U. Wo b u s & H.P. Mo c k : Mapping of quantitative trait loci Universität Hohenheim, Hohenheim (2011) 99 pp. associated with protein expression variation in barley grains. Ka s p a r , S.: Analysis of isolated barley tissues using proteomic Mol. Breed. 27 (2011) 301-314. approaches. (PhD Thesis) Martin-Luther-Universität Halle- Wittenberg, Halle/S. (2011) 224 pp. La n g e , H.: Modifikation von Anthocyanseitenketten mit Hilfe rekombinant exprimierter Proteine. (Bachelor Thesis) Hoch- schule Anhalt, Köthen (2011) 75 pp.

135 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

Research Group: Structural Cell Biology Head: Dr. Michael Melzer

Scientists vacuolated immature uni-nucleate barley pollen until the development of multicellular structures at high temporal IPK financed resolution. Nine different developmental types of cultured Rutten, Twan, Dr. pollen have been determined (Fig. 46) including two pathways characteristic for pollen undergoing POEM. Ultrastructural Grant Positions and live-cell imaging results identified nuclear fusion as Daghma, Diaa El-Din (0.50 BMBF, till 30.11.2010) the crucial mechanism of genome doubling during barley pollen embryogenesis. It can occur at any developmental stage Visiting Scientists/Scholars during pollen embryogenesis and multiple fusions may lead Andersen, Louise, Prof. (self-financed, 28.04.-17.05.2011) to variable ploidy level in multicellular structures (Fig. 47, p. Ghoniem, Ahmed Eisa Mahmoud (Cairo University, 10.10.- 137). The first mitosis of the uninucleate microspores can be 09.11.2010) symmetric or asymmetric. Although this is important for the Marzec, Marek (EU-UPGOW, till 29.03.2010; DAAD, 01.04.- future pathway we found that it is not a key trigger of POEM. 21.04.2011, 02.06.-28.06.2011 and 29.09.-20.10.2011) The advanced experimental set up and the comprehensive in- Pandey, Pooja (DAAD-Siemens fellowship, 24.09.-30.09.2011) formation on the initial mechanisms of POEM will eventually allow functional validation of genes putatively involved in POEM and therefore stimulate a knowledge-based establishment of the commercially useful haploid technology in numerous plant Goals species.

As core facility for light and electron microscopy the Structural Cell Biology group on the one hand provides practical and theoretical advice to other groups that wish to address research problems by microscopy and conducts own research projects on the other hand. In various cooperations with internal and external research groups we focus mainly on ultrastructural characterisation, monitoring of cell dynamic processes and the spatial distribution of macromolecules in plants using sophisticated cell biological techniques of confocal laser scanning microscopy (CLSM), spinning disc microscopy (SDM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

Research Report

To understand the initial mechanisms of pollen embryogenesis in barley (D. S. Daghma, GABI-POEM), various cell biological methods have been used to identify structural markers Fig. 46 and features of the embryogenic and gametophytic pathway. Time lapse of type I development (embryogenic pollen). CLSM images shown in DIC and nuclear GFP fluorescence. (a) Uni-nucleate pollen grain with the Ultrastructural observations showed that both gametophytic nucleus close to the pollen aperture. (b, c) Migration of the nucleus to a semi- and embryogenic pollen contain a large central vacuole, which central position and formation of cytoplasmic strands. The blurred fluorescence signal seen in (c) indicates the disruption of the nuclear envelope before mitosis. persisted beyond the first pollen mitosis. The gametophytic (d) Newly formed cell wall (DIC) and two daughter nuclei (GFP) after mitosis. pathway is characterised by the gradual decreasing of the large (e) Appearance of cytoplasmic strands indicating imminent second mitosis. (f, g) Newly formed cell walls (DIC) separate four cells (GFP) contained within the central vacuole, while in pollen embryogenesis this central vac- original exine. (h) Additional cycles of mitosis have created multicellular structure. uole was gradually replaced by many small ones. As compared CS, cytoplasmic strands; CW, cell wall; N, nucleus; n, nucleolus; PA, pollen aperture. Bar = 20 µm (D. S. Daghma, T. Rutten, G. Hensel, J. Kumlehn and M. Melzer). to embryogenic pollen, gametophytic pollen had a higher density of ribosomes, rough endoplasmic reticulum, and organelles such as mitochondria and dictyosomes. The development of a custom-made live cell imaging chamber and the use of transgenic barley lines with GFP expression in the nucleus for the first time allowed the visualisation of POEM from the

136 organisation within the female gametophyte. In the centromeres of all eukaryotes, the normal histone H3 is replaced with a centromere-specific variant,CENH 3 in plants. The presence of CENH3 is believed to be important for the assembly of the kinetochore on the centromere which is responsible for a correct chromosome segregation during nuclear division. In collaboration with the research group Karyotype Evolution (I. Lermontova) the dynamics of CENH3 in root tip cells of Arabidopsis thaliana were investigated. Using live cell imaging approaches we were able to confirm a plant-specific behaviour of CENH3 dynamics during the cell cycle. In animals CENH3 is stable throughout the cell cycle until the anaphase. During this short time of increased mobility, just before the end of mitosis, animal cells duplicate their CENH3 content. In Arabidopsis root tip cells expressing YFP-tagged CENH3, fluorescence intensity together with CENH3 content remained stationary for at least three to four hours prior to mitosis. A mere 7 % of the total protein content was mobile during this time, indicating a slow turnover that may compensate for damaged molecules, or the presence of two separate CENH3 populations which have yet to be identified. After mitosis each daughter nucleus contained exactly half of the fluorescence intensity of the mother nucleus. These data prove that in Arabidopsis CENH3 loading must happen very early in G2. The loading process itself, our analysis indicates, may last no more than 30 min. To follow CENH3 dynamics during the complete cell cycle of single cells in real time, long-term observations using a recently acquired spinning disc microscope will be carried out. Together with the research group Molecular Plant Nutrition (M. Hajirezaei) and the Institute of Molecular and Cell Biology Fig. 47 of the National University of Rosario, Argentina (M. Zurbriggen, Nuclear fusion in multicellular structures 7 days after initiation of POEM. N. Blanco, N. Carillo) we continued our structural characterisa- Transmission electron microscopy (a-c) and confocal laser scanning microscopy (d-e). (a-b) Elongated nucleus with clear median constriction. (c) Detail of nucleus tion of transgenic tobacco plants expressing a cyanobacterial cytoplasmic pockets and a narrow median band (dashed box) marking the site of flavodoxin (Fld). fusion. (d) DAPI staining. (e) GFP expression in nuclei with highly polyploid nuclei (arrows) next to small likely haploid nuclei (stars). Cy, cytoplasm; CyP, cytoplasmic pocket; ER, endoplasmic reticulum; MVB, multi vesicles body; N, nucleus; Ne, In a joint project (DAAD) with the Dept. of Genetics, Katowice, Nuclear envelope; V, vacuole (D. S. Daghma, T. Rutten, G. Hensel, J. Kumlehn and M. Melzer). Poland (I. Szarejko, M. Marzec) we are working on the structural and molecular characterisation of barley root epidermal cells for the identification of new genes involved in root hair development in Hordeum vulgare. Preliminary studies have In collaboration with the research group Gene Regulation shown a different localisation pattern of arabinogalactans in (D. Köszegi, M. Michael) a break-through was realised in eluci- epidermal cells of control and hairless mutant barley plants. dating the role of the egg cell-specific transcription factors AtRKD1 and AtRKD2. Permanent induction of these factors arrest the function of the shoot apical meristem and cause the initiation of axillary meristems. Expression analysis of the meristem-specific transcription factors WUSCHEL and CLAVATA tagged with GUS or GFP showed that meristematic identity was not altered in the arrested shoot apical meristem. However, studies with the auxin-inducible promoter DR5 revealed that the auxin gradient typical for normal meristems, was absent in plants overexpressing ATRKD1 or AtRKD2. The conclusion that AtRKD1 and AtRKD2 may play a role in regulating auxin gradients is supported by recent publications showing locally produced auxin gradients to be essential for the correct cellular

137 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

Publications Da g h m a , D.S., J. Ku m l e h n & M. Me l z e r : The use of cyanobacteria as filler in nitrocellulose capillaries improves ultrastructural Peer Reviewed Papers preservation of immature barley pollen upon high pressure freezing. J. Microsc. 244 (2011) 79-84. 2010 Ka r i m i As h t i y a n i , R., A.M. Ba n a e i Mo g h a d d a m , V. Sc h u b e r t , T. Ru t t e n , Ag a r w a l , R., A. Ma t r o s , M. Me l z e r , H.P. Mo c k & J.K. Sainis: J. Fu c h s , D. De m i d o v , F.R. Bl a t t n e r & A. Ho u b e n : AtHaspin Heterogeneity in thylakoid membrane proteome of phosphorylates histone H3 at threonine 3 during mitosis Synechocystis 6803. J. Proteomics 73 (2010) 976-991. and contributes to embryonic patterning in Arabidopsis. Ar s o v a , B., U. Ho j a , M. Wimmelbacher , E. Gr e i n e r , S. Üs t ü n , M. Me l z e r , Plant J. 68 (2011) 443-454. K. Pe t e r s e n , W. Le i n & F. Bö r n k e : Plastidial thioredoxin z interacts Kő s z e g i , D., A.J. Jo h n s t o n , T. Ru t t e n , A. Cz i h a l , L. Al t s c h m i e d , J. with two fructokinase-like proteins in a thiol-dependent Ku m l e h n , S.E. Wu s t , O. Kirioukhova, J. Gh e y s e l i n c k , U. Grossniklaus manner: Evidence for an essential role in chloroplast & H. Bä u m l e i n : Members of the RKD transcription factor family development in Arabidopsis and Nicotiana benthamiana. induce an egg cell-like gene expression program. Plant J. 67 Plant Cell 22 (2010) 1498-1515. (2011) 280-291. Jo h n s t o n , A.J., O. Kirioukhova, P.J. Ba r r e l l , T. Ru t t e n , J.M. Mo o r e , Le r m o n t o v a , I., O. Ko r o l e v a , T. Ru t t e n , J. Fu c h s , V. Sc h u b e r t , I. Mo r a e s , R. Ba s k a r , U. Grossniklaus & W. Gr u i s s e m : Dosage-sensitive D. Kö s z e g i & I. Sc h u b e r t : Knockdown of CENH3 in Arabidopsis function of RETINOBLASTOMA RELATED and convergent reduces mitotic divisions and causes sterility by disturbed epigenetic control are required during the Arabidopsis life meiotic chromosome segregation. Plant J. 68 (2011) 40-50. cycle. PLoS Genet. 6 (2010) e1000988. Le r m o n t o v a , I., T. Ru t t e n & I. Sc h u b e r t : Deposition, turnover, and Ko t s e r u b a , V., K. Pistrick, F.R. Bl a t t n e r , K. Ku m k e , O. We i s s , T. Ru t t e n , release of CENH3 at Arabidopsis centromeres. Chromosoma J. Fu c h s , T. En d o , S. Na s u d a , A. Gh u k a s y a n & A. Ho u b e n : The 120 (2011) 633-640. evolution of the hexaploid grass Zingeria kochii (Mez) Tzvel. Me l k u s , G., H. Ro l l e t s c h e k , J. Fu c h s , V. Ra d c h u k , E. Gr a f a h r e n d -Be l a u , (2n=12) was accompanied by complex hybridization and N. Sreenivasulu , T. Ru t t e n , D. We i e r , N. He i n z e l , F. Sc h r e i b e r , T. uniparental loss of ribosomal DNA. Mol. Phylogenet. Evol. 56 Al t m a n n , P.M. Ja k o b & L. Bo r i s j u k : Dynamic (13) C/(1) H NMR (2010) 146-155. imaging uncovers sugar allocation in the living seed. Plant Lo l a s , I.B., K. Hi m a n e n , J.T. Gr ø n l u n d , C. Ly n g g a a r d , A. Ho u b e n , Biotechnol. J. 9 (2011) 1022-1037. M. Me l z e r , M. va n Lijsebettens & K.D. Gr a s s e r : The transcript Ru d o l p h , M., A. Sc h l e r e t h , M. Kö r n e r , K. Fe u s s n e r , E. Be r n d t , M. elongation factor FACT affects Arabidopsis vegetative and Me l z e r , E. Ho r n u n g & I. Fe u s s n e r : The lipoxygenase-dependent reproductive development and genetically interacts with oxygenation of lipid body membranes is promoted by a HUB1/2. Plant J. 61 (2010) 686-697. patatin-type phospholipase in cucumber cotyledons. J. Exp. Wi e d e m a n n , G., C. He r m s e n , M. Me l z e r , A. Bu t t n e r -Mainik, H. Bot. 62 (2011) 749-760. Re n n e n b e r g , R. Re s k i & S. Ko p r i v a : Targeted knock-out of a gene Ts c h i e r s c h , H., L. Bo r i s j u k , T. Ru t t e n & H. Ro l l e t s c h e k : Gradients of encoding sulfite reductase in the mossPhyscomitrella patens seed photosynthesis and its role for oxygen balancing. affects gametophytic and sporophytic development. FEBS Biosystems 103 (2011) 302-308. Lett. 584 (2010) 2271-2278.

2011 PhD and Diploma Theses Bl a n c o , N.E., R.D. Ce c c o l i , M.E. Se g r e t i n , H.O. Po l i , I. Vo s s , M. Me l z e r , 2011 F.F. Br a v o -Al m o n a c i d , R. Sc h e i b e , M.R. Ha j i r e z a e i & N. Ca r r i l l o : Da g h m a , D.S.: Structural changes during the initiation of pollen Cyanobacterial flavodoxin complements ferredoxin embryogenesis in barley (Hordeum vulgare L.). (PhD Thesis) deficiency in knocked-down transgenic tobacco plants. Martin-Luther-Universität Halle-Wittenberg, Halle/S. (2011) Plant J. 65 (2011) 922-935. 167 pp. Cz a r n e c k i , O., B. He d t k e , M. Me l z e r , M. Ro t h b a r t , A. Ri c h t e r , Y. Sc h r ö t e r , T. Pfannschmidt & B. Gr i m m : An Arabidopsis GluTR-binding protein mediates spatial separation of 5-aminolevulinic acid synthesis in chloroplasts. Plant Cell 23 (2011) 4476-4491.

138 Research Group: Plant Reproductive Biology Head: Dr. Jochen Kumlehn

Scientists Goals

IPK financed The major interest of the Plant Reproductive Biology group Daghma, Diaa El-Din (0.50 Pakt für Forschung und Innovation, centers around the establishment and implementation of ena- 01.07.-31.12.2011) bling technologies such as genetic transformation, micro-dis- Goedeke, Stefanie (0.50 Pakt für Forschung und Innovation, section of live cells and the generation of true-breeding plants till 31.03.2010) so as to facilitate applied research as well as crop improvement Gurushidze, Maia, Dr. (0.25, till 31.08.2010; 0.50/0.75, 01.04.- approaches. Taking advantage of the resultant technological 31.08.2011) platform, further research projects aim to elucidate mecha- Hensel, Götz, Dr. (Pakt für Forschung und Innovation, nisms of sexual and asexual plant reproduction, plant-patho- 01.09.2010-28.02.2011) gen interactions, as well as many other aspects of crop plant Kapusi, Eszter (Pakt für Forschung und Innovation, performance. till 31.05.2011) Rizzo, Paride (0.50, 01.01.-31.03.2010) Saalbach, Isolde, Dr. (0.50) Research Report

Grant Positions Pollen embryogenesis is a viable means to produce popula- Berger, Carolin, Dr. (BMBF, till 23.09.2010) tions of entirely true-breeding plants. Whereas those plants are Bruchmüller, Astrid (0.50 Overhead, till 31.03.2010) highly valuable both in research and practical breeding, the Gurushidze, Maia, Dr. (0.50/0.75 BMBF, till 30.04.2011; 0.25 Over- molecular processes that are crucial for the initiation of pol- head, 01.05.-31.08.2011; 0.50/1.00 BMBF, since 01.09.2011) len embryogenesis are largely unkown. In cooperation with Guse, Tilo (0.50 Industry) KWS SAAT AG and the IPK research groups Structural Cell Bi- Hensel, Götz, Dr. (BMBF, till 31.08.2010; since 01.03.2011) ology, Gene Regulation, Applied Biochemistry as well as Bio- Kastner, Christine (0.50 BMBF) informatics and Information Technology, we have thus been Pencs, Stefanie (BMBF, since 01.11.2011) pursuing an integrated research approach that encompasses Plasun, Katarzyna (0.50 BMBF, till 31.08.2010, 0.50 Overhead, ultrastructural studies, time-lapse microscopy as well as com- 01.09.-31.12.2010) parative transcript, metabolite and protein profiling using the Riechen, Jan (0.50 BMBF, till 31.01.2011) model species tobacco and barley. To this end, the PRB group Rizzo, Paride (0.50 Overhead, 01.04.-30.09.2010; 0.50 BMBF, has been providing highly specific samples of developmental 01.10.-31.12.2010) stages of both regular pollen development and pollen embryogenesis, which was achieved by improvements in pollen treat- Visiting Scientists/Scholars ment, purification of pollen populations, and individual pollen Bini, Federica, Dr. (self-financed, since 01.09.2011) selection. Deep sequencing of mRNA isolated from those sam- Cambra, Inés (University of Madrid, 11.04.-14.07.2011; ples has resulted in a unique resource for the identification IPK, 15.08.-14.09.2011) and functional validation of genes putatively involved in Gajecka, Monika (ERASMUS fellowship, 06.06.-05.09.2011) the developmental switch from pollen maturation towards Goedeke, Stefanie (self-financed, 01.04.-31.12.2010) embryogenesis. The long-term goal of this work is to facilitate Hiekel, Stefan (IPK, 10.10.-30.11.2011) knowledge-based development of haploid technology broadly Plasun, Katarzyna (self-financed, since 01.01.2011) applicable in crop plants (D.S. Daghma, G. Hensel, I. Otto). Pourkheirandish, Mohammad, Dr. (NIAS, 03.01.-08.01.2011) Perera, Prasanthi, Dr. (CIAT, 23.09.-23.10.2011) Szyrajew, Katarzyna (ERASMUS fellowship, 19.09.-18.12.2011) Tedeschi, Francesca, Dr. (University of Tuscia, 01.10.2010- 30.09.2011) Zahid Abbas, Malik (Fellowship of Higher Education Commis- sion, 17.06.-16.12.2010)

139 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

Among the Triticeae cereals, rye is considered to be the most The PRB group’s transformation platform that includes Agro- recalcitrant as to pollen embryogenesis. In a bilateral project bacterium-based methods for barley, wild barley (Hordeum with the plant breeding station KWS Lochow, we have recently spontaneum), wheat, rye, triticale, maize and tef has been ex- succeeded to establish embryogenic pollen cultures and to tended to the emerging monocot model species Brachypo- generate doubled haploid lines in this species (C. Bollmann, dium distachyon. This was achieved by a novel method that S. Hiekel). Moreover, we were the first to generate plants via the rests on the formation of regenerable callus on segments of pollen embryogenesis pathway in the Poaceae model species primary shoots from mature, in vitro germinated grains. This Brachypodium distachyon. However, in the most important method has the major advantages that there is no need to con- plant model Arabidopsis, our efforts to establish haploid tech- tinuously grow donor plants to provide recipient explants used nology have failed so far. Though we were able to develop a for the gene transfer, and that the dissection of shoot segments highly efficient method to produce plants from in vitro culti- is much more straightforward than the isolation of the tiny im- vated anthers, these lines proved by molecular markers to origi- mature embryos of Brachypodium (B. Melzer, T. Guse, Ch. Kast- nate from somatic cells of the anther rather than from haploid ner, C. Berger, E. Kapusi, G. Hensel). pollen grains (B. Melzer, K. Plasun, I. Otto, P. Hoffmeister). To study molecular genetic mechanisms functionally associat- Conventional TILLING (Targeting Induced Local Lesions in Ge- ed with apomixis, the genus Boechera is an ideal model system. nomes) populations derive from mutagenic treatment of dip- As it is characterised by naturally occurring diploid apomictic loid tissues, which results in the formation of heterozygotic and sexual forms, gene expression of both reproductive modes mutations and chimeric primary mutant lines. Therefore, we can be compared without the confounding effects of polyploi- have pursued a novel approach where haploid single cells, i.e. dy. In co-operation with the Apomixis group, we have isolated isolated immature pollen (microspores) of barley, are used as ovules from sexual and apomictic accessions at different a target for chemical mutagenesis. This enables us to gener- stages of development to identify differentially expressed ate homozygous, non-chimeric primary mutant lines that can genes after deep sequencing of respective cDNA libraries. To be instantly identified by phenotype and reproduced without allow for the functional validation of candidate genes asso- any segregation. A short-awned, as well as a dwarf pheno- ciated with apomictic reproduction, we recently succeeded as type did not segregate in the M2 generation indicating that the first to establish a transformation method for Boechera the respective mutations had already been homozygous and polyantha. The protocol rests on the inoculation of petiols of in non-chimeric in the primary mutant plants. To evaluate the vitro germinated seedlings with Agrobacterium (S. Freist, I. Otto, frequency of induced mutations, embryogenic pollen cultures G. Hensel). of transgenic cv. Igri lines carrying a homozygous, single-copy green fluorescence protein (GFP) gene were subjected to mutagenesis treatment. It was demonstrated that GFP knock-out mutations can be already visually detected in haploid pollen- derived embryo populations (Fig. 48). (M. Gurushidze, F. Bini, E. Kugelmann, I. Otto).

Fig. 48 Mutagenisation via ethane methyl sulfonate treatment of isolated barley microspores derived from a transgenic line carrying a single GFP copy at homozygous state. left: embryogenic pollen culture from non-treated microspores with GFP being consistently expressed in all embryo-like structures. right: pollen-derived embryos following EMS-treatment of microspores, upper right: embryos under white light, lower right: excitation of the same objects with far blue light reveals that the GFP gene appears to be knocked out in the individuum placed in the centre, while the left and right ones show fluorescence (Photos: M. Gurushidze and J. Kumlehn).

140 Engineered minichromosomes open up the opportunity to RNAi is an established means of knocking down genes in simultaneously transfer and stably express multiple transgenes plants and fungi. Both sequence-specificity as well as systemic in plants without recombination of the genetic background. In spreading of gene silencing is essentially mediated by small collaboration with the research groups Chromosome Structure interfering RNAs. Considering that there is intimate cellular and Function and Epigenetics, we have truncated Arabidopsis contact between plants and fungal pathogens during infec- and barley chromosomes via integration of telomere se- tion, we hypothesised that fungal genes may effectively be quences using Agrobacterium, which is a first crucial step in a targeted by small RNAs derived from appropriately designed top-down approach towards the development of minichromo- hairpin constructs expressed by host plant cells. This phenom- somes. In barley, de novo formation of telomeres was found in enon that was coined host-induced gene silencing (HIGS) tetraploid plants only, indicating that the recovery of shortened has been investigated in cooperation with the Transcriptome chromosomes is facilitated by genetic redundancy. Truncated Analysis group employing the barley-powdery mildew patho- chromosomes were transmissible in sexual reproduction, system. Transgenic barley lines were generated using a hairpin but were inherited at rates lower than expected according to the RNAi construct based on a fragment of the B. graminis hor- Mendelian rules (E. Kapusi, G. Hensel, S. Knüpffer,C . Marthe). dei glucanosyltransferase 1 (GTF1) gene. GTFs are specifically In collaboration with the Technical University Munich we previ- found in fungi where they are involved in cell elongation and ously showed that the barley ROP G-protein RACB is required virulence. In T1-populations obtained, colony formation of B. for full susceptibility of the leaf epidermis to invasion by the graminis hordei was significantly reduced. The results suggest biotrophic fungus Blumeria graminis f. sp. hordei. More recently, uptake of RNA molecules by the powdery mildew fungus from we demonstrated that the barley MICROTUBULE-ASSOCIAT- attacked plant cells, which may cause knock-down of targeted ED ROP-GTPASE ACTIVATING PROTEIN (MAGAP1) labelled fungal genes and reduced disease severity. Thanks to its poten- with GFP decorated cortical microtubules and was recruited tially high specificity, the huge array of candidate target genes, by activated RACB to the cell periphery. Under fungal attack, and the fact that the protection mechanism acts without any MAGAP1-labelled microtubules establish a polarised network further gene product being required, this novel principle holds at sites of successful defence. These results suggest that RACB a promise for future applications to effectively combat fungal and MAGAP1 might be antagonistic players in cytoskeleton diseases of crop plants (G. Hensel, G. Zimmermann, A. Knospe, organisation in the context of fungal infection. In a further ap- C. Marthe). proach with the same collaboration partner, transgenic barley plants carrying an HvBI-1 RNA-interference (RNAi) construct To functionally validate maize genes with regard to their role were generated. These lines had reduced levels of HvBI-1 tran- in the interaction with Ustilago maydis, a virus-induced gene si- script and showed enhanced resistance to penetration by B. lencing (VIGS) system based on the Brome Mosaic Virus (BMV) graminis hordei. Reactive oxygen species (ROS) also play an im- was established in maize by our cooperation partners at the portant role in plant-pathogen interactions. Prominent sources MPI in Marburg. Using this system, transient knock-down of the of ROS are respiratory burst oxidase homologs (RBOH) whose terpene synthase 6/11 (TPS6⁄11) gene that had been found to function is still poorly understood. Again in collaboration with be significantly upregulated upon U. maydis infection resulted the Technical University Munich, transgenic barley RNAi-lines in increased severity of disease symptoms. In order to confirm were generated, which showed reduced expression of the these results and to demonstrate the value of the newly es- HvRBOHF2 isoform of NADPH oxidases, failed to establish pen- tablished VIGS system, we produced stably transgenic maize etration resistance to B. graminis hordei and to control leaf cell lines carrying a ubiquitously expressed TPS6⁄11 RNAi-construct. death (G. Hensel, C. Marthe). Plants where tps6⁄11 expression was effectively silenced also showed significantly increased levels of U. maydis colonisation associated with increased tumor formation and extended chlorosis, as was found in the respective VIGS experiment (Ch. Kast- ner, S. Wolf, H. Büchner).

141 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

Publications He n s e l , G., A. Hi m m e l b a c h , W. Ch e n , D.K. Do u c h k o v & J. Ku m l e h n : Transgene expression systems in the Triticeae cereals. J. Peer Reviewed Papers Plant Physiol. 168 (2011) 30-44. Ho e f l e , C., C. Hu e s m a n n , H. Sc h u l t h e i s s , F. Bö r n k e , G. He n s e l , J. Ku m l e h n 2010 & R. Hü c k e l h o v e n : A barley ROP GTPase ACTIVATING PROTEIN Ei c h m a n n , R., M. Bi s c h o f , C. We i s , J. Sh a w , C. La c o m m e , P. Sc h w e i z e r , D. associates with microtubules and regulates entry of the bar- Do u c h k o v , G. He n s e l , J. Ku m l e h n & R. Hü c k e l h o v e n : BAX INHIBI- ley powdery mildew fungus into leaf epidermal cells. Plant TOR-1 is required for full susceptibility of barley to powdery Cell 23 (2011) 2422-2439. mildew. Mol. Plant Microbe Interact. 23 (2010) 1217-1227. Kő s z e g i , D., A.J. Jo h n s t o n , T. Ru t t e n , A. Cz i h a l , L. Al t s c h m i e d , J. Ku m - Hi m m e l b a c h , A., L. Liu, U. Zi e r o l d , L. Al t s c h m i e d , H. Ma u c h e r , F. Be i e r , l e h n , S.E. Wu s t , O. Kirioukhova, J. Gh e y s e l i n c k , U. Grossniklaus & D. Mü l l e r, G. He n s e l , A. He i s e , A. Schützendübel , J. Ku m l e h n & P. H. Bä u m l e i n : Members of the RKD transcription factor family Sc h w e i z e r : Promoters of the barley germin-like GER4 gene induce an egg cell-like gene expression program. Plant J. 67 cluster enable strong transgene expression in response to (2011) 280-291. pathogen attack. Plant Cell 22 (2010) 937-952. Te o , C.H., L. Ma, E. Ka p u s i , G. He n s e l , J. Ku m l e h n , I. Sc h u b e r t , A. Ho u b e n Ke m p e , K., M. Ru b t s o v a , C. Be r g e r , J. Ku m l e h n , C. Sc h o l l m e i e r & M. Gi l s : & M.F. Me t t e : Induction of telomere-mediated chromosomal Transgene excision from wheat chromosomes by phage truncation and stability of truncated chromosomes in Arabi- phiC31 integrase. Plant Mol. Biol. 72 (2010) 673-687. dopsis thaliana. Plant J. 68 (2011) 28-39. Me l o n e k , J., M. Mu l i s c h , C. Sc h m i t z -Li n n e w e b e r , E. Gr a b o w s k i , G. He n - v a n d e r Li n d e , K., C. Ka s t n e r , J. Ku m l e h n , R. Ka h m a n n & G. Do e h l e m a n n : s e l & K. Kr u p i n s k a : Whirly1 in chloroplasts associates with in- Systemic virus-induced gene silencing allows functional char- tron containing RNAs and rarely co-localises with nucleoids. acterisation of maize genes during biotrophic interaction Planta 232 (2010) 471-481. with Ustilago maydis. New Phytol. 189 (2011) 471-483. No w a r a , D., A. Ga y , C. La c o m m e , J. Sh a w , C. Ri d o u t , D. Do u c h k o v , G. He n s e l , J. Ku m l e h n & P. Sc h w e i z e r : HIGS: host-induced gene silencing in the obligate biotrophic fungal pathogen Blumeria Books and Book Chapters graminis. Plant Cell 22 (2010) 3130-3141. Pr o e l s , R.K., K. Oberhollenzer , I.P. Pa t h u r i , G. He n s e l , J. Ku m l e h n & R. 2010 Hü c k e l h o v e n : RBOHF2 of barley is required for normal devel- Ku m l e h n , J., G. Zi m m e r m a n n , C. Be r g e r , C. Ma r t h e & G. He n s e l : Char- opment of penetration resistance to the parasitic fungus acters of transgenic plants and their application in plant Blumeria graminis f. sp. hordei. Mol. Plant Microbe Interact. production – Triticeae cereals. In: Ke m p k e n , F. & C. Ju n g (Eds.): 23 (2010) 1143-1150. Genetic modification of plants – Agriculture, Horticulture & Ra d c h u k , R., U. Co n r a d , I. Sa a l b a c h , M. Gi e r s b e r g , R.J. Em e r y , H. Kü s t e r , Forestry. Springer-Verlag, Berlin-Heidelberg (2010) 287-306. A. Nu n e s -Ne s i , A.R. Fe r n i e , W. We s c h k e & H. We b e r : Abscisic acid 2011 deficiency of developing pea embryos achieved by immu- He n s e l , G.: Genetic transformation of Triticeae cereals for mo- nomodulation attenuates developmental phase transition lecular farming. In: Al v a r e z , M. (Ed.): Genetic Transformation. and storage metabolism. Plant J. 64 (2010) 715-730. ISBN: 978-953-307-364-4, InTech (2011) 171-190, Available Sh a r b e l , T.F., M.L. Vo i g t , J.M. Co r r a l , G. Ga l l a , J. Ku m l e h n , C. Kl u k a s , F. from: http://www.intechopen.com/articles/show/title/ Sc h r e i b e r , H. Vo g e l & B. Ro t t e r : Apomictic and sexual ovules of genetic-transformation-of-triticeae-cereals-for-molecular- Boechera display heterochronic global gene expression pat- farming. terns. Plant Cell 22 (2010) 655-671. We i c h e r t , N., I. Sa a l b a c h , H. We i c h e r t , S. Ko h l , A. Er b a n , J. Ko p k a , B. Ha u s e , A. Va r s h n e y , N. Sreenivasulu , M. St r i c k e r t , J. Ku m l e h n , W. PhD and Diploma Theses We s c h k e & H. We b e r : Increasing sucrose uptake capacity of 2010 wheat grains stimulates storage protein synthesis. Plant Go e d e k e , S.: Transgene Gerstenkaryopsen als Produktionssystem Physiol. 152 (2010) 698-710. von pharmazeutisch relevanten Proteinen – Beeinflussung der Akkumulation durch regulatorische Elemente. (PhD The- 2011 sis) Martin-Luther-Universität Halle-Wittenberg, Naturwis- Ab e r a , B., L. Ne g a s h , J. Ku m l e h n & T. Fe y i s s a : In vitro regeneration of senschaftliche Fakultät I, Halle/S. (2010) 142 pp. Taverniera abyssinica A. Rich – a threatened medicinal plant. Ka p u s i , E.: Elimination of selectable markers genes via segregation Ethiop. J. Educ. Sci. 6 (2011) 59-71. of uncoupled T-DNAs in populations of doubled haploid bar- Da g h m a , D.S., J. Ku m l e h n & M. Me l z e r : The use of cyanobacteria ley. (PhD Thesis) Martin-Luther-Universität Halle-Wittenberg, as filler in nitrocellulose capillaries improves ultrastructural Naturwissenschaftliche Fakultät I, Halle/S. (2010) 103 pp. preservation of immature barley pollen upon high pressure freezing. J. Microsc. 244 (2011) 79-84. 2011 Gu g s a , L. & J. Ku m l e h n : Somatic embryogenesis and massive Me l z e r , B.: Transformation von Brachypodium distachyon durch shoot regeneration from immature embryo explants of tef. den Agrobacterium-vermittelten Gentransfer in Sprossseg- Biotechnol. Res. Int. 2011 (2011) Article ID 309731, 7 pages, mentkallus. (Diploma Thesis) Hochschule Mittweida, (2011) doi:10.4061/2011/309731. 166 pp.

142 Research Group: Yeast Genetics Head: Prof. Gotthard Kunze

Scientists and filamentous fungi serve as sources for genes suitable for metabolic redesign of plants in order to improve the quality of IPK financed end products or to develop recombinant microbes as biosen- Jankowska, Dagmara (0.50, since 01.12.2011) sors to detect environmental pollutions. Moreover arbuscular Pham, Thi Minh Ha (0.50, since 01.08.2011) mycorrhizal fungi (AMF) are in the centre of interest to analyse Sedzielewska, Kinga Anna (0.50, since 01.04.2011) and optimise fungus-plant root interactions. They are used as Trautwein, Anke (0.50, since 01.12.2011) a gene source to produce biotechnologically interesting AMF proteins improving plant growth even under stress conditions. Grant Positions Böer, Erik, Dr. (Industry, till 31.03.2010; BMBF, 01.04.2010- 28.02.2011) Research Report Chamas, Alexandre (0.50 BMBF, since 01.05.2011) Florschütz, Kristina, Dr. (AIF) Arxula adeninivorans is an attractive non-pathogenic organism Gerlach, Torsten (0.50 BMBF) for basic and applied research. Its biotechnological application Giersberg, Martin, Dr. (AIF) results from special and remarkable characteristics, such as im- Hähnel, Urs, Dr. (Industry, since 17.05.2010) mense broad range of sole carbon and nitrogen sources, inter- Kasprzak, Jakub (0.50 Industry, since 15.11.2011) esting temperature-dependent dimorphism, extreme thermo-, Körner, Martina, Dr. (AIF, till 23.06.2010) osmo- and salt tolerance, excellent growth and secretion char- Riechen, Jan (0.50/1.00 Industry, since 01.07.2011) acteristics. The genome of Arxula is completely sequenced and Schwarz, Maria (0.50 AIF, since 15.04.2010) annotated enabling to explore and exploit new pathways such Sedzielewska, Kinga Anna (0.50 Overhead, 15.09.2010- as the metabolism of tannic acid, purine and n-butanol. Fur- 31.03.2011) thermore A. adeninivorans is used as host for the synthesis of Trautwein, Anke (0.50 AIF, till 30.11.2011) special products such as recombinant glycosylated secretory Worch, Sebastian, Dr. (AIF, since 01.02.2011) proteins. It serves as a suitable biocatalyst for the synthesis of biotechnological interesting products such as n-butanol, be- Visiting Scientists/Scholars cause all essential prerequisites and components for heterolo- Alvaro Benito, Miguel (CBMSO scholarship Madrid, gous gene expression are available. Several specialised meth- 23.08.-26.11.2010) ods have been established (transformation/expression plat- Baronian, Keith, Prof. (self-financed, 17.06.-28.06.2010) form Xplor®2, gene disruption, protoplast fusion, mitotic Lemanski, Tabias Samuel (ComEAST, 08.02.-07.05.2010) segregation) and industrial strains were constructed. These Nutz, Sabine (IPK, 01.02.-04.03.2011) strains are suitable producers of enzymes to substitute chem- Ordenes, Viviana, Dr. (self-financed, 11.10.-25.10.2010) ical synthesis steps by biochemical steps (1), to produce the Pham, Thi Minh Ha (MOET Vietnam, till 31.07.2011) glycoprotein glomalin as potential formulation additive, soil Sashidar, Burla, Dr. (DLR, 08.04.-28.06.2010) conditioner and product for the pharmacology (2), to improve Sedzielewska, Kinga Anna (AMykor GmbH, till 14.09.2010) the biogas content (3) and to produce food with low pu- Toro Nahuelpan, Mauricio (Fellowship of Bayer Foundation, rine content (4). Other biotechnological application fields for 19.08.2010-03.03.2011) Arxula cells are the accumulation of metal ions, production Yelchuri, Ravi Kumar (BMBF, 16.11.-10.12.2010) of biobutanol and its use as biocatalyst of a microbial fuel cell (MFC) that produces high levels of a reduced molecule to shuttle electrons from the cell to the electrode. This can serve as an alternative for conventional energy production in the fu- Goals ture (D. Jankowska, A. Trautwein, M. Schwarz, E. Böer, U. Hähnel, J. Riechen, K. Florschütz, S. Worch, A. Kahlo, M. Kunze, K. Giese, The research of the Yeast Genetics group focuses on the devel- M. Benito Alvaro, J.B. del Pino). opment of yeast as a model organism to study osmo- and salt In addition A. adeninivorans is used as model organism for tolerance. Yeast is a valuable tool to characterise metabolic plants. Investigations in the field of osmo- and salt tolerance pathways in eukaryotes and is able to synthesise biotechno- are performed within the intergroup project “Molecular analy- logically interesting products. In addition they are used as mi- sis of salt tolerance in barley”. This group focuses on the key crobial compounds of biosensors. Yeast species that are used pathways for osmo- and salt tolerance and on the identifica- are Saccharomyces cerevisiae and non-Saccharomyces yeasts tion of compatible solutes in barley and yeast. Barley and such as Arxula adeninivorans and Hansenula polymorpha. Both A. adeninivorans cDNA sequences encoding for products improv- are completely sequenced and have annotated genomes. Yeast ing osmo- and salt tolerance are identified by complementation

143 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

Fig. 49 A. activation of reporter gene expression. after binding of a xenobiotic element (Drug A) to the PX receptor, the receptor heterodimerises with its partner RXR and binds to the xenobiotic response element (ER6) located on the modified GAA-promoter. Alternatively, the human Cyp3A4 promoter was used to initiate specific reporter gene expression C( yp3A4 yeast clones) in yeast cells. B. Schematic illustration of the yeast biosensor workflow. C. Reaction of the new biosensor cells exposed to different Diclofenac concentrations. cells were incubated over night in different Di- clofenac concentrations at 30 °C under constant shaking conditions. Afterwards the activity of the secreted phytase was determined by a colorimetric enzyme assay.

144 of the osmo- and salt-sensitive yeast S. cerevisiae. The analysis Beside yeasts, arbuscular mycorrhizal fungi (AMF) are in the of the selected A. adeninivorans genes including gene products focus of the research group. They are able to establish a sym- demonstrate that the HOG pathway is induced by two ways: (1) biotic relationship with 70 - 90 % of land plant species and ob- by phosphorylation of relevant enzymes as shown for yeast with viously, this interaction has a major impact on the entire soil moderate resistance and (2) by induction of HOG genes, such ecosystem. They improve the uptake of phosphorus and nitro- as ASLN1, AYPD1, ASSK1, ASTE20, the MAPKK kinases ASTE11 and gen by plants and are essential to protect plants against root ASSK2, the MAPK kinase APBS2 and the MAP kinase AHOG1. The pathogens and able to improve salt and drought tolerance. phosphorylated Ahog1p induces the expression of genes en- DNA content of AMF strains were determined by flow cytom- coding the synthesis of compatible solutes, such as glycerol, etry and confirmed afterwards by Feulgen densitometry. Fur- erythritol and mannitol. Whereas the levels of glycerol and eryth- thermore, fluorescence in situ hybridisation (FISH) was used to ritol correlate directly with the osmolarity of the culture media, determine the number of rDNA loci per genome. The two ana- intracellular mannitol is accumulated to a very high extent and lysed Glomus isolates (AMykor and DAOM 197198) belong to is relatively independent of the medium osmolarity. This effect, Glomus irregulare species, have a genome size of approximately together with the combination of phosporylation and gene in- 150 Mb and obtained 1 or 2 rDNA loci per genome. For the tax- duction, seems to provide better adaptation during transition onomic analysis of AMF, for the identification and classification from low to high osmolarity conditions (A. Trautwein, M. Dähne, of mycorrhiza residing on plant roots and the detection of phy- E. Böer, P. Mock – Applied Biochemistry group, M. Melzer –Struc- topathogenic viruses on barley and potato biosensors based tural Cell Biology group). on DNA-DNA and DNA-RNA hybridisation, as well as antibody- Yeasts are also used as microbial sensor compound for the antigen interaction have been developed and adapted. Based detection of hormone activities (estrogenic, androgenic, on DNA hybridisation, detection assays in the 96-well format progesteronic, glucocorticoidic activities), dioxins as well and microchips based on the surface plasmon resonance as pharmaceuticals in tap water, mineral water, waste water, technique, are able to analyse the mycorrhizal content in plant urine and blood serum. Sensors are based on recombinant A. populations and phytopathogenic viruses in plants. The micro- adeninivorans cells which include the respective human recep- titer plate assay has been validated and applied in the analysis tors (hERa, hAR, hPR, hGR, AHR, PXR / RXR) and are designed of the mycorrhizal content in a wood population growing un- as estrogen/androgen/progesterone/glucocorticoid/dioxin/ der extreme conditions (K. Florschütz, K. Sedzielewska, D. Klee- pharmaceutical screen assays with biochemical measurement mann, A. Schröter, G. Oswald – Fig. 50, p. 146). as well as microbial biosensors with an amperometric detection method. The estrogen and androgen screen assay (A- YES and A-YAS assay) have been validated for its suitability to measure estrogens and estrogen-like substances as well as androgens and androgen-like substances in samples of tap water, mineral water, urine and different wastewater treatment plants which have high matrix effects. With detection limits of approx. 0.5 ng l-1 for 17 ß-estradiol (E2) and 80 ng l-1 for 5a-di- hydrotestosterone (DHT) the assays are applicable for a range of 0.5 to 80 ng l-1 effective E2 and 80 – 400 ng l-1 DHT concentrations, respectively. First assays (A-YES®_aqua, A-YAS®_aqua, A_YES®_aqua_salutaris) based on these sensor compounds are commercialised already. A parallel receptor functionalised microchips based on the surface plasmon resonance technique is established, which detect receptor-ligand interactions. Therefore the respective recombinant receptors are produced by the Xplor®2 transformation/expression platform in yeast (M. Giersberg, P. Thi Minh Ha, T. Gerlach, A. Chamas, C. Kaiser, A. Ni- eter, J. Saß, H. Greif, – see Fig. 49, p. 144).

145 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

Fig. 50 A Life cycle of an arbuscular mycorrhizal fungus belonging to Glomus irregulare species. B histograms of relative fluorescence intensities obtained after flow cytometry analysis of propidium iodide-stained nuclei of (a) G. irregulare DAOM197198, (b) G. irregulare AMykor, (c) G. irregulare DAOM197198 (grey) with internal reference standard A. thaliana ‘Col-O’ (black), (d) G. irregulare AMykor (grey) with internal reference standard A. thaliana ‘Col-O’ (black). Due to endoreduplication occurring in A. thaliana leaf tissue nuclei (Galbraith et al., 1991), different ploidy levels are detectable. The first peak represents 2C nuclei with a DNA content of 0.32 pg. Fluorescence intensity of nuclei is expressed in arbitrary units (linear scale).

Publications Kn a b b e n , I., L. Re g e s t e i n , C. Gr u m b a c h , S. St e i n b u s c h , G. Ku n z e & J. Bü c h s : Online determination of biomass in high cell-density Peer Reviewed Papers processes of Arxula adeninivorans in two different minimal media using an impedance signal. J. Biotechnol. 149 (2010) 2010 60-66 Gi e r s b e r g , M., D.M. Fl o s s , S. Kipriyanov, U. Co n r a d & J. Sc h e l l e r : Co- Ra d c h u k , R., U. Co n r a d , I. Sa a l b a c h , M. Gi e r s b e r g , R.J. Em e r y , H. Kü s t e r , valent dimerization of camelidae anti-human TNF-alpha A. Nu n e s -Ne s i , A.R. Fe r n i e , W. We s c h k e & H. We b e r : Abscisic acid single domain antibodies by the constant kappa light chain deficiency of developing pea embryos achieved by immu- domain improves neutralizing activity. Biotechnol. Bioeng. nomodulation attenuates developmental phase transition 106 (2010) 161-166. and storage metabolism. Plant J. 64 (2010) 715-730. Ka i s e r , C., S. Uh l i g , T. Ge r l a c h , M. Kö r n e r , K. Si m o n , K. Ku n a t h , K. Si e t m a n n , R., R. Ue b e , E. Bö e r , R. Bo d e , G. Ku n z e & F. Sc h a u e r : Novel Fl o r s c h ü t z , K. Ba r o n i a n & G. Ku n z e : Evaluation and validation metabolic routes during the oxidation of hydroxylated aro- of a novel Arxula adeninivorans estrogen screen (nAES) assay matic acids by the yeast Arxula adeninivorans. J. Appl. Micro- and its application in analysis of wastewater, seawater, brack- biol. 108 (2010) 789-799. ish water and urine. Sci. Total Environ. 408 (2010) 6017-6026. Wi t z e l , K., A. We i d n e r , G.K. Su r a b h i , R.K. Va r s h n e y , G. Ku n z e , G.H. Bu c k - Ka u r , P., B. Si n g h , E. Bö e r , N. St r a u b e , M. Pi o n t e k , T. Satyanarayana & So r l i n , A. Bö r n e r & H.P. Mo c k : Comparative analysis of the G. Ku n z e : Pphy-A cell-bound phytase from the yeast Pichia grain proteome fraction in barley genotypes with contrast- anomala: Molecular cloning of the gene PPHY and charac- ing salinity tolerance during germination. Plant Cell Environ. terization of the recombinant enzyme. J. Biotechnol. 149 33 (2010) 211-222. (2010) 8-15.

146 2011 PhD and Diploma Theses Bö e r , E., F.S. Br e u e r , M. We n i g e r , S. De n t e r , M. Pi o n t e k & G. Ku n z e : 2010 Large-scale production of tannase using the yeast Arxula ad- Ka i s e r , C.: Etablierung eines auf Hefezellen basierenden As- eninivorans. Appl. Microbiol. Biotechnol. 92 (2011) 105-114. says zur Erfassung östrogener Wirkungen im Rahmen der Ch e l i k a n i , V., F.J. Ra w s o n , A.J. Do w n a r d , R. Go o n e r a t n e , G. Ku n z e , N. Umwelt-, Nahrungs- und Futtermittelanalytik. (PhD Thesis) Pa s c o & K.H.R. Ba r o n i a n : Electrochemical detection of oestro- Ernst-Moritz-Arndt-Universität, Greifswald (2010) 175 pp. gen binding protein interaction with oestrogen in Candida Kl e m a n n , D.: Genetic diversity of arbuscular mycorrhizal fungi albicans cell lysate. Biosens. Bioelectron. 26 (2011) 3737- (AMP) and the influence of AMF on salt tolerance in Medi- 3741. cago truncatula. (Master Thesis) Universität Hohenheim, Ho- Ha s l e t t , N.D., F.J. Ra w s o n , F. Ba r r i e r e , G. Ku n z e , N. Pa s c o , R. Go o n e r a t - henheim (2010) 98 pp. n e & K.H.R. Ba r o n i a n : Characterisation of yeast microbial fuel Mo l i t o r , R.: Isolation und Charakterisierung der aus Arxula cell with the yeast Arxula adeninivorans as the biocatalyst. adeninivorans stammenden Gallussäure-Decarboxylase Biosens. Bioelectron. 26 (2011) 3742-3747. (GSDC)- und Dihydroxybenzoate-Decarboxylase (ADBC)- Sêdzielewska , K.A., J. Fu c h s , E.M. Te m s c h , K. Ba r o n i a n , R. Wa t z k e & G. Gene. (Bachelor Thesis) Hochschule Anhalt (FH), Köthen Ku n z e : Estimation of the Glomus intraradices nuclear DNA (2010) 84 pp. content. New Phytol. 192 (2011) 794-797. Ni e t e r , A.: Entwicklung eines auf Hefezellen basierenden Bio- Si n g h , B., G. Ku n z e & T. Satyanarayana : Developments in biochemi- sensors zum Nachweis von Substanzen mit glucocorticoider cal aspects and biotechnological applications of microbial Wirkung. (Bachelor Thesis) Martin-Luther-Universität Halle- phytases. Biotechnol. Mol. Biol. Rev. 6 (2011) 69-87. Wittenberg, Halle/S. (2010) 49 pp. Wo r c h , S., K. Ra j e s h , V.T. Harshavardhan , C. Pi e t s c h , V. Ko r z u n , L. Ku n t z e , A. Bö r n e r , U. Wo b u s , M.S. Rö d e r & N. Sreenivasulu : Hap- 2011 lotyping, linkage mapping and expression analysis of barley Dä h n e , M.: Untersuchungen des HOG-Pathways bei Hefen. genes regulated by terminal drought stress influencing seed (Bachelor Thesis) Hochschule Anhalt (FH), Köthen (2011) 59 quality. BMC Plant Biol. 11 (2011) 1. pp. Gi e s e , K.: Tanninabbau in Arxula adeninivorans – Charak- terisierung und Funktionsanalyse der Tannase 2 (Atan2p). Books and Book Chapters (Bachelor Thesis) Hochschule Anhalt (FH), Köthen (2011) 104 pp. 2010 Gr e i f , H.: Nachweis der Pregnan-X-(PXR)-, Retinoid-X-(RXR)- Fl o r s c h ü t z , K., A. Sc h r ö t e r , M. Kö r n e r , S. Sc h m i e d e r , F. So n n t a g , K. Ve t - Rezeptor Heterodimerisierung durch bimolekulare Fluo- t e r , R. Wa t z k e & G. Ku n z e : Auf den Spuren von Pilzen und Viren reszenz-Komplementation. (Bachelor Thesis) Hochschule mittels SPR und Plattenassay. 15. Heiligenstädter Kolloqui- Anhalt (FH), Köthen (2011) 83 pp. um “Technische Systeme für die Lebenswissenschaften”, 27.- Ka h l o , A.: Enzyme als Biokatalysatoren zur Synthese von Zuck- 29.09.2010. (2010) 185-192. erderivaten für die Biomedizin und Biotechnologie. (Bach- elor Thesis) Hochschule Anhalt (FH), Köthen (2011) 77 pp. 2011 Fl o r s c h ü t z , K., F. So n n t a g , A. Sc h r ö t e r , S. Sc h m i e d e r , U. Kl o t z b a c h & G. Ku n z e : On-Chip-Nachweis von phytopathogenen RNA- Viren und miRNA mittels Oberflächenplasmonenresonanz. Patents In: Proc. Sensorforschung für Medizin und Technik. Sensor + 2010 Test (2011) 35-40. Ku n z e , G., K. Fl o r s c h ü t z , R. Wa t z k e , K. Wa t z k e & R. Ha c k e r : „Verfah- Ge r l a c h , T., C. Ch e r k o u k , L. Re b o h l e , S. Ho w i t z , G. Ha n k e , P. Le h m a n n , ren zum Nachweis von Mykhorrizapilzen“. Anmeldetag: M. Jä h n e , K. Si m o n , S. Uh l i g & G. Ku n z e : Rezeptor-selektive Anrei- 21.04.2009, Offenlegung: 08.12.2010, IPK Nr. 2009 / 03. cherung und Bio-LED-Sensorik zur qualitativen und quanti- Ku n z e , G., K. Fl o r s c h ü t z & M. Kö r n e r : „Biosensor und Verfahren zur tativen Detektion von östrogen- und androgen-wirkenden Regelung einer Wasseraufbereitungsanlage“. Anmeldetag: Substanzen. In: Proc. 7. Deutsches Biosensorsymposium, 25.06.2009, Offenlegung: 30.12.2010, IPK Nr. 2009 / 05. Heiligenstadt (2011) 58.

147 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

Research Group: Systems Biology Head: Dr. Björn Junker

Scientists

IPK financed Bundtzen, Sophie, Dr. (0.50, 01.06.-31.12.2011) Poskar, Hart, Dr. (01.02.-14.05.2010)

Grant Positions Baker, Syed Murtuza (0.50 BMBF) Bundtzen, Sophie, Dr. (0.50 Industry, 15.11.2010-31.05.2011) Franke, Mathias (0.50 BMBF) Hüge, Jan, Dr. (BMBF, since 01.08.2010) Krach, Christian, Dr. (BMBF) Liiving, Tiina (0.50 BMBF) Poskar, Hart, Dr. (Industry, since 15.05.2010) Schallau, Kai, Dr. (BMBF, till 31.10.2011)

Goals

Understanding the dynamics of plant metabolic pathways, especially in seeds of crop plants, by a combination of computer modeling and biochemical analysis. Fig. 51 A map of intracellular metabolic fluxes of developing pea seeds (H. Poskar, C. Krach). Research Report

The major scientific focus of the group is the metabolism of For steady-state metabolic flux analysis several experimental plant seeds, which is investigated by applying systems biol- and computational parts of the method had to be established ogy methods. Mathematical models of metabolism are cre- in the group: embryo cultures, stable isotope feeding experi- ated, and the experimental data necessary for these models ments and sample preparation (N. Schäfer, F. Kellner, L. Ficht- is generated. Thus, the work is divided approximately 50/50 müller), analysis by GC-MS (J. Hüge), automatic data extraction into experimental methods for data generation and theoretical and correction for natural isotope abundance (H. Poskar, M. methods for data processing and prediction. Franke), as well as model creation for pea and Medicago central metabolism (C. Krach). The resulting flux maps cover large parts The group is funded by a 5-year BMBF grant as a FORSYS-Part- of central metabolism and resolve cyclic and parallel fluxes (see ner Junior Research Group since Juli 2008. In the last two years, Fig. 51). several state-of-the-art methods have been established and are Due to the highly complex and laborious nature of steady-state now used as the work horses of the group. The main method is metabolic flux analysis, in the past the method has been applied steady-state metabolic flux analysis, in which computational to small numbers of genotypes or treatments (usually not more models of plant metabolism are enriched by stable isotope label- than 3 per study). We have significantly increased the throughput ling data obtained from the lab. This method has been applied to of the method by using lab automation, simplifying the labora- investigate metabolic processes taking place at the filling stage in tory protocols, and automating the computational pipeline from legume seeds, especially pea (Pisum sativum) and barrel medic data extraction to flux modelling. Currently, we have studies un- (Medicago truncatula), as well as cereal seeds, above all barley der way for flux comparison of more than 100 genotypes. Thus, (Hordeum vulgare) and rice (Oryza sativa). the screening of medium-scale plant populations for individuals with desired flux phenotypes is now possible.

148 In the reported period, several stoichiometric and kinetic mod- 2011 els have been set up for seed metabolism of different species Ba k e r , S.M., C.H. Po s k a r & B.H. Ju n k e r : Unscented Kalman filter (K. Schallau, S. Weber, K. Lotz, S.M. Baker), covering central me- with parameter identifiability analysis for the estimation of tabolism of the seeds. These models are constantly filled by ex- multiple parameters in kinetic models. EURASIP J. Bioinform. perimental data, which is derived from metabolite and enzyme Syst. Biol. 2011 (2011) 7. measurements (N. Schäfer, F. Kellner, V. Peter). For pea seeds, we Hu e g e , J., J. Go e t z e , D. Sc h w a r z , H. Ba u w e , M. Ha g e m a n n & J. Ko p k a : have set up a non-aqueous technique to analyse metabolites Modulation of the major paths of carbon in photorespira- and enzymes in a sub-cellular way (T. Liiving). For the kinetic tory mutants of Synechocystis. PLoS One 6 (2011) e16278. models, parameters that could not be measured may be esti- Ju n k e r , B.H.: Steady-state-13C-Flussanalysen in Pflanzen. Bio- mated by a new computational pipeline for parameter identi- spektrum 17 (2011) 230-232. fiability analysis and parameter estimation with an improved We i s e , S., J. Ch e n , B.H. Ju n k e r , M. Klapperstück , M. La n g e , F. Sc h r e i b e r algorithm tailored for biochemical data (S.M. Baker). With the & U. Sc h o l z : Das Informationssystem MetaCrop zur Unter- help of the kinetic models, targets for metabolic engineering stützung systembiologischer Forschung an Kulturpflanzen. can be predicted to improve seed composition. We have start- it - Information Technology 53 (2011) 234-240. ed to verify the predicted strategies by generating the respective genetic constructs and transforming them into plants. First results from these plants will be obtained in 2012. Books and Book Chapters

2010 Ba k e r , S.M., H. Po s k a r & B.H. Ju n k e r : Unscented Kalman filter for Publications estimation of multiple parameters in kinetic models. In: Ny k - t e r , M., P. Ru u s u v u o r i , C. Ca r l b e r g & O. Yl i -Ha r j a (Eds.): Proc. 7th Peer Reviewed Papers Intl. Conf. on Computational Systems Biology (WCSB2010), June 16-18, Luxembourg. Tampere International Center for 2010 Signal Processing. TICSP series 51 (2010) 19-22. Ba k e r , S.M., K. Sc h a l l a u & B.H. Ju n k e r : Comparison of different algorithms for simultaneous estimation of multiple para- 2011 meters in kinetic metabolic models. J. Integr. Bioinform. 7 Liiving, T., S.M. Ba k e r & B.H. Ju n k e r : Biochemical fundamentals. In: (2010) e133. Ko c h , I., W. Re i s i g & F. Sc h r e i b e r (Eds.): Modeling in systems biol- Fl o s s , D.M., K. Sc h a l l a u , S. Ro s e -Jo h n , U. Co n r a d & J. Sc h e l l e r : Elas- ogy: the petri net approach. Springer Book Series Computa- tin-like polypeptides revolutionize recombinant protein ex- tional Biology, Vol. 16. Springer, New York, USA (2011) 19-36. pression and their biomedical application. Trends Biotech- Sc h u s t e r , S. & B.H. Ju n k e r : Topological analysis of metabolic and nol. 28 (2010) 37-45. regulatory networks. In: Ko c h , I., W. Re i s i g & F. Sc h r e i b e r (Eds.): Hi p p e , K., C. Co l m s e e , T. Cz a u d e r n a , E. Gr a f a h r e n d -Be l a u , B.H. Ju n k e r , Modeling in systems biology: the petri net approach. Sprin- C. Kl u k a s , U. Sc h o l z , F. Sc h r e i b e r & S. We i s e : Novel developments ger Book Series Computational Biology, Vol. 16. Springer, of the MetaCrop information system for facilitating systems New York, USA (2011) 209-224. biological approaches. J. Integr. Bioinform. 7 (2010) e125. Ko s c h ü t z k i , D., B.H. Ju n k e r , J. Sc h w e n d e r & F. Sc h r e i b e r : Structural analysis of metabolic networks based on flux centrality. J. Theor. Biol. 265 (2010) 261-269. Sc h a l l a u , K. & B.H. Ju n k e r : Simulating plant metabolic pathways with enzyme-kinetic models. Plant Physiol. 152 (2010) 1763- 1771. Sreenivasulu , N., L. Bo r i s j u k , B.H. Ju n k e r , H.P. Mo c k , H. Ro l l e t s c h e k , U. Se i f f e r t , W. We s c h k e & U. Wo b u s : Barley grain development toward an integrative view. Int. Rev. Cell Mol. Biol. 281 (2010) 49-89.

149 Abteilung Physiologie und Zellbiologie/Department of Physiology and Cell Biology

Other Papers 2011 2010 Sc h r e i b e r , F., C. Co l m s e e , T. Cz a u d e r n a , E. Gr a f a h r e n d -Be l a u , A. Ha r t - Ba k e r , S.M. & B.H. Ju n k e r : Adaptive unscented Kalman filter for m a n n , A. Ju n k e r , B.H. Ju n k e r , D. Ko s c h ü t z k i , U. Sc h o l z & S. We i s e : estimation of parameters in kinetic metabolic models. Proc. MetaCrop 2.0. http://metacrop.ipk-gatersleben.de (2011). 4th Int. Conf. on Computational Systems Biology (2010) 185- We i s e , S., I. Gr o s s e , C. Kl u k a s , D. Ko s c h ü t z k i , U. Sc h o l z , F. Sc h r e i b e r & 186. B.H. Ju n k e r : Meta-All: A system for managing metabolic path- Le m k e , R., M. Ha j i r e z a e i , B.H. Ju n k e r , J. Mü l l e r, B. Us a d e l , M. Le p s & F. way information. http://meta-all.ipk-gatersleben.de (2011). Sc h r e i b e r : MMM – Mehrskalen-Stoffwechselmodelle zur Sys- tembiologie in Getreiden – ein integrativer Ansatz für die Biomasseforschung. Systembiologie.de 2 (2010) 51-53. PhD and Diploma Theses 2011 Pe t e r , V.: Vorhersage der subzellulären Verteilung von Enzymak- Electronic Publications tivitäten im Erbsensamen auf Basis von Expressionsdaten. 2010 (Bachelor Thesis) Hochschule Mittweida (2011) 79 pp. Gr a f a h r e n d -Be l a u , E., S. We i s e , D. Ko s c h ü t z k i , U. Sc h o l z , B.H. Ju n k e r We b e r , S.: Validierung einer neuen Enzymkinetik-Approximation & F. Sc h r e i b e r : MetaCrop – A detailed database of crop plant am Citratzyklus von Erbsensamen (Diploma Thesis) Otto- metabolism. http://metacrop.ipk-gatersleben.de (2010). von-Guericke-Universität, Magdeburg (2011) 263 pp. We i s e , S., I. Gr o s s e , C. Kl u k a s , D. Ko s c h ü t z k i , U. Sc h o l z , F. Sc h r e i b e r & B.H. Ju n k e r : Meta-All: A system for managing metabolic pathway information. http://meta-all.ipk-gatersleben.de (2010).

150 Pflanzengenom-Ressourcen- Plant Genome Resources Centrum (PGRC) Centre (PGRC) Koordinator: Dr. habil. Patrick Schweizer Coordinator: Dr. Patrick Schweizer

Das Pflanzengenom-Ressourcen-Centrum (PGRC; http://pgrc. The Plant Genome Resources Centre (PGRC; http://pgrc.ipk- ipk-gatersleben.de/) des IPK erbrachte in den Jahren 2010 und gatersleben.de/) of the IPK provided services in the field of 2011 Serviceleistungen für interne Nutzer und externe Koope- plant genomics to internal users and external cooperation rationspartner, diente als wissenschaftlich-technische Platt- partners in 2010 and 2011, served as platform for scientific form am IPK und koordinierte das internationale Forschungs- exchange and coordinated BarleyGenomeNet (http://www. netzwerk BarleyGenomeNet (http://www.barleynet.org) für barleynet.org), an international network of institutions with a Genomforschung der Gerste. Die Organisation des PGRC be- main research focus on barley genomics and genetics. The in- findet sich momentan in einem Prozess der Erneuerung, wie ternal structure of the PGRC is currently under re-organisation unten stehend weiter ausgeführt werden soll. as outlined below.

Für den wissenschaftlichen Forschritt der zum PGRC gehören- 1. Service: den Arbeitsgruppen wird auf die Jahresberichte der jeweiligen Service and support by the PCRC was extended by SNP analysis Gruppen verwiesen. using the medium-throughput VeraCode system (Illumina Co.) in 2010 and in 2011 by population development and -mainte- 1. Service: nance for barley, wheat and (if requested) Arabidopsis thaliana. Die Serviceleistungen des PGRC wurden 2010 um SNP-Analyse These species were nominated by group leaders in 2010. On unter Verwendung des VeraCode-Systems der Firma Illumi- the other hand, it was decided not to continue TILLING in bar- na und 2011 um Populationsaufbau/Erhalt für Gerste, Weizen ley as a PGRC service after the GABI-TILL (BMBF) project came und (bei Bedarf) für Arabidopsis thaliana erweitert. Hingegen to an end although this was initially considered. Instead, out- wird TILLING der Gerste nach Beendigung der entsprechenden sourcing of the barley TILLING resource to a commercial service Drittmittelförderung (GABI-TILL) nicht, wie ursprünglich erwo- provider is considered. The PGRC technician who had support- gen, in der Form eines PGRC-Service weitergeführt, sondern ed GABI-TILL and thus provided TILLING service to internal and soll allenfalls an einen kommerziellen Serviceprovider ausge- external users has been re-integrated into the PGRC core team lagert werden. Der entsprechende Einsatz von PGRC-Personal and is now responsible person for the new population service. wurde beendet und stellte somit eine Projektunterstützung Because of a strong decline of requests for membrane spotting dar, die diversen internen und externen Nutzern der TILLING- this service is being terminated. The same applies to the frag- Plattform zugutekam und dementsprechend Querschnittscha- ment analysis service on capillary sequencer MegaBACE1000. rakter hatte. Die Mitarbeiterin wird seit 2011 im neuen Service In general, it can be expected that within the next two years „Populationsaufbau/Erhalt“ eingesetzt. Infolge stark zurück- service activities will continue to shift toward sequence-based gegangener Nachfrage nach Membranspotting wurde dieser analyses by Sanger- and especially next-generation sequenc- Service formell beendet. Allgemein wird für die Zukunft eine ing using ABI 3730, Roche FLX and Illumina HiSeq 2000 instru- noch stärkere Verlagerung der Serviceaktivitäten in Richtung ments together with specific research groups. Sequenzierung unter Verwendung herkömmlicher Sanger- als auch NGS-Technologien (ABI 3730, Roche FLX, Illumina HiSeq 2. Scientific-technical organisation: 2000) erwartet, bei gemeinsamer Nutzung der Geräte durch The initial organisation of the PGRC in seven method-orient- das PGRC-Serviceteam und durch einzelne Arbeitsgruppen. ed research modules did not optimally meet the information needs of IPK scientists and shall therefore be formally replaced 2. Wissenschaftlich-technische Organisation: by a task-force structure by the end of 2011. These task forces Die bisherige Organisation des PGRC in sieben methodenorien- are aimed at bringing together scientific expertise for fields tierte Module konnte den Informationsbedarf im Hause nicht of general interest, to foster the flow of information including optimal befriedigen und soll daher zum Ende 2011 durch die know-how transfer between scientific groups at the institute, inzwischen geschaffenen thematischen „task forces“ wissen- and to develop common strategies for the acquisition of large schaftlicher Arbeitsgruppen formell abgelöst werden. Ziel ist instruments, grant proposals, cooperations etc. The following die Zusammenführung von Expertise in bestimmten Themen- task forces exist to date: bereichen von arbeitsgruppenübergreifendem Interesse, ein effizienter Informationsfluss inklusive Know-how-Transfer zwi- • Next Generation Sequencing (NGS, Coord. N. Stein) schen Wissenschaftlern im Hause, und die Erarbeitung gemein- • Association Genetics (Coord. B. Kilian) samer Strategien für Gerätebeschaffungen, Antragsstellungen, • Stress Integration (Coord. P. Schweizer) interner und externer Kooperationen etc. Folgende Task Forces • Phenomics (Coord. T. Altmann) existieren zurzeit: • Metabolite Analysis (Coord. H.-P. Mock).

151 Pflanzengenom-Ressourcen-Centrum (PGRC)/Plant Genome Resources Centre (PGRC)

• Next Generation Sequencing (NGS, Koord. N. Stein) The task forces have organised a number of workshops and • Assoziationsgenetik (Koord. B. Kilian) seminars involving either all IPK scientists or group leaders. The • Stressintegration (Koord. P. Schweizer) formation of an additional task force “Microscopy” should be • Phenomics (Koord. T. Altmann) evaluated in the near future. • Metabolite Analysis (Koord. H.-P. Mock) 3. National and international networks: Die Task Forces veranstalteten eine Reihe von Arbeitstreffen The international network of institutions with a major interest und/oder Seminaren, die sich an alle wissenschaftlichen Mit- in barley genomics and genetics, BarleyGenomeNet, continues arbeiter oder an Gruppenleiter richteten. Die Schaffung einer to be coordinated by the PGRC leader P. Schweizer. After about weiteren Task Force „Mikroskopie“ soll eruiert werden. eight years of existence, BarleyGenomeNet proved to be a fruit- ful platform for bi- or multilateral core-funded collaborations 3. Nationale und internationale Netzwerke: aiming at joint publications or generation of proof of concept Das internationale Netzwerk von Institutionen mit schwer- data that are important for coordinated grant proposals. Unfor- punktmäßigem Interesse an der Genomforschung der Gerste, tunately, a joint proposal for a Marie Curie Research and Train- BarleyGenomeNet, wird weiterhin vom PGRC-Leiter P. Schwei- ing Network (ITN) about the multiple roles of abscisic acid in zer koordiniert. Nach rund acht Jahren des Bestehens erweist barley was not successful in 2011 but will be improved and re- sich BarleyGenomeNet als fruchtbare Plattform für grundfinan- submitted in 2012. This will be discussed among other issues zierte bi- respektive multilaterale Kooperationen mit dem Ziel at the upcoming annual network meeting taking place at The gemeinsamer Publikation oder der Einreichung koordinierter James Hutton Institute in Dundee in November 2011. Other Projektanträge. Leider wurde ein 2011 zum zweiten Mal einge- relevant networks such as the International Barley Sequencing reichter Antrag zur Förderung eines Marie-Curie-Projektes (ITN, Consortium (IBSC) and the COST action “TritiGen” are being co- integrated training and research network) des Netzwerkes zur ordinated or supported by PGRC scientists. vielfältigen Rolle von Abscisinsäure in der Gerste nicht bewilligt, soll aber 2012 nochmals in verbesserter Form eingereicht For scientific progress achieved in research groups belong- werden. Die BarleyGenomeNet-Partner werden am 24./25. No- ing to the PGRC, please read the reports of the corresponding vember 2011 am James Hutton Institute in Dundee zum näch- groups. sten Jahrestreffen zusammenkommen, um unter anderem den ITN-Antrag zu planen und weitere Fördermöglichkeiten zu er- örtern. Patrick Schweizer, November 2011 Weitere für das PGRC bedeutsame Netzwerke wie das Interna- tionale Gerstengenom-Sequenzierungskonsortium (IBSC) und die COST-Aktivität „TritiGen“ wurden mit maßgeblicher Beteili- gung von PGRC-Mitgliedern weitergeführt.

Patrick Schweizer, November 2011

152 Die Entwicklung der The Development of Bioinformatik am IPK Bioinformatics at IPK Koordinator: Prof. Dr. Falk Schreiber Coordinator: Prof. Falk Schreiber

In den Jahren 2010/2011 hat sich die Bioinformatik kontinuier- In 2010/2011 bioinformatics has continuously developed, en- lich zu inzwischen sechs Arbeitsgruppen weiterentwickelt, da- compassing now six bioinformatics research groups including von zwei rein drittmittelfinanzierte Nachwuchsgruppen. Diese two solely third-party funded junior research groups. They cov- decken ein breites Spektrum bioinformatischer und systembio- er a wide spectrum of bioinformatics and systems biology re- logischer Forschung ab, das von Datenbanken und Informati- search, which ranges from databases and information systems onssystemen über Datenanalyse, Modellierung und Simulation to data analysis, modelling and simulation, to visualisation. In a bis zur Visualisierung reicht. Die Bioinformatik am IPK ist in viel- diversity of interdisciplinary projects, bioinformatics at the IPK fältigen interdisziplinären Projekten eng mit der biologischen is closely integrated with biological research at the institute, Forschung am Institut, aber auch mit externen Partnern ver- and also with many external partners. With regards to the sci- netzt. Für den wissenschaftlichen Fortschritt der Arbeitsgrup- entific progress of the individual research groups and their col- pen und deren Kooperationen wird auf die Berichte der jeweili- laborations, see the reports of the respective groups. gen Gruppen verwiesen. As part of their work the bioinformatics groups develop and Durch die Bioinformatikgruppen werden unter anderem Da- host databases, information systems and software tools for tenbanken, Informationssysteme, und Software-Werkzeuge für plant research. Examples for databases and information sys- die pflanzenbiologische Forschung entwickelt und unterhal- tems are the Genebank information system for the manage- ten. Beispiele für Datenbanken und Informationssysteme sind ment of accessions in the Genebank, the European Barley Da- das Genbankinformationssystem für die Verwaltung und tabase with over 150,000 accessions of European barley collec- das Management der Muster in der Genbank, die European tions and three non-European collections as well as evaluation Barley-Database mit über 150.000 Akzessionen europäischer data, the Mansfeld-Datenbank for crop plant taxonomical Gerstensammlungen und dreier außereuropäischer Sammlun- information on 6,100 plant species cultivated worldwide, and gen sowie Evaluierungsdaten, die Mansfeld-Datenbank für MetaCrop with data about metabolic pathways of different kulturpflanzentaxonomische Informationen zu 6.100 weltweit crop and model plants. Important bioinformatics tools are VAN- kultivierten Pflanzenarten und MetaCrop mit Daten zu me- TED for the visualisation and analysis of complex biochemical tabolischen Pathways diverser Kultur- und Modellpflanzen. data sets, especially *omics data in the context of biological Herausragende Bioinformatik-Tools sind VANTED zur Ansicht networks; SBGN-ED for the creation, editing, validation, and und Analyse von komplexen biochemischen Datensätzen, be- export of maps in the Systems Biology Graphical Notation sonders von *omics-Daten im Kontext biologischer Netzwerke, standard; FBASimVis for the reconstruction of stoichiometric SBGN-ED zum Erstellen, Ändern, Validieren und Exportieren models, their analysis with Flux Balance Analysis, and the ex- von Diagrammen entsprechend dem Systems Biology Graphi- plorative visualisation of analysis results; IAP for the processing cal Notation Standard, FBASimVis zur Rekonstruktion stöchio- of image data from IPK high-throughput phenotyping systems; metrischer Modelle, deren Analyse mittels Flux Balance Analy- Jstacs for statistical analysis and sequence classification; and sis und der explorativen Visualisierung der Analyseergebnisse, Lailaps, a system for the search in non-integrated life science IAP zur Verarbeitung der Bilddaten der Hochdurchsatz-Phäno- databases. typisierungssysteme am IPK, Jstacs für statistische Analysen und Sequenzklassifikation, und Lailaps, ein System zur Suche in nicht integrierten Life Science-Datenbanken.

153 Die Entwicklung der Bioinformatik am IPK/The Development of Bioinformatics at IPK

Um die Bioinformatik als Einheit zu stärken und über das IPK To strengthen bioinformatics as a unit and increase its visibil- hinaus sichtbar werden zu lassen, haben die Bioinformatik- ity beyond IPK, in addition to their own work, the bioinformat- gruppen neben den eigenen Arbeiten eine Vielzahl gemein- ics groups have carried out a large number of joint activities. samer Aktivitäten durchgeführt. Erwähnenswert sind hier das Worth mentioning here are: the bioinformatics module for the gemeinsam von allen Gruppen durchgeführte Modul Bioin- study programme in AgriGenomics at the Christian Albrecht formatik für den AgriGenomics-Studiengang an der Christian- University Kiel; the international conferences International Albrechts-Universität zu Kiel; die (Mit)organisation internatio- Symposium on Integrative Bioinformatics 2010 from 20 to naler Tagungen wie International Symposium on Integrative 22 March 2010 in Cambridge (UK), International Symposium Bioinformatics 2010 vom 20. bis 22. März 2010 in Cambridge on Integrative Bioinformatics 2011 from 21 to 23 March 2011 (UK), International Symposium on Integrative Bioinforma- in Wageningen (The Netherlands), and International Work- tics 2011 vom 21. bis 23. März 2011 in Wageningen (Nieder- shop on Systems Biology Graphical Notation from 21 to 23 lande) und des International Workshop on Systems Biology April 2010 at the Leucorea Wittenberg. Graphical Notation vom 21. bis 23. April 2010 in der Leucorea Wittenberg. Bioinformatics at IPK will continue to grow and develop in the future, reflecting the importance of this field of research to Auch in den nächsten Jahren soll der erfolgreiche Kurs fortge- IPK as a whole. The challenge will be on the one hand to con- führt und die Bioinformatik am IPK im Kontext der biologischen tinue to expand bioinformatics at IPK into new areas not yet Forschung am Institut weiterentwickelt werden. Eine Heraus- covered, on the other hand to continue to uphold successful forderung ist dabei einerseits die Stärkung von Bereichen, die research areas, for example, in the junior research groups for durch die Bioinformatik bisher nicht ausreichend abgedeckt which funding is not continuous. All those interested in bioin- sind, andererseits die Verstetigung erfolgreicher Forschungs- formatics are warmly invited to an upcoming conference which gebiete beispielsweise in den auslaufenden Nachwuchsgrup- is co-organised by IPK bioinformatics: the International Sym- pen. Zu erwähnen ist auch eine Tagung im nächsten Jahr, die posium on Integrative Bioinformatics from 2 to 4 April 2012 von der Bioinformatik mit organisiert wird und zu der alle In- in Hangzhou (China). teressierten bereits jetzt herzlich eingeladen sind: das Inter- national Symposium on Integrative Bioinformatics vom 2. bis 4. April 2012 in Hangzhou (China). Falk Schreiber, November 2011

Falk Schreiber, November 2011

154 Das Doktorandenprogramm The PhD Programme Sprecherin: Nicole Schmid Speaker: Nicole Schmid

Derzeit forschen über 80 Doktoranden am Institut und sind More than 80 PhD students study and work at the IPK and durch das PhD Student Board (PSB) organisiert. Das PSB are organised by the PhD Student Board (PSB). The PSB was wurde vor ca. sieben Jahren gegründet und ist in einzelne founded about seven years ago and is structured into different Aufgabenbereiche unterteilt. Hauptaufgabe dieser selbstorga- task forces. The main responsibility of this self-organised PhD nisierten Doktorandenvertretung ist die Durchführung eines student representatives is the implementation of the PhD de- promotionsbegleitenden Programms am IPK sowie die Organi- velopment programme at IPK as well as the organisation of so- sation gemeinschaftlich orientierter Veranstaltungen. Seit 2008 cial events. Since 2008, PSB managed its own budget. It is used verfügt das PSB über ein eigenes Budget, über welches ein viel- to finance PhD development programme consisting of diverse fältiges Angebot an Seminaren, Exkursionen, Workshops etc. fi- events such as seminars, study trips and workshops and thereby nanziert wird und damit die wissenschaftliche Ausbildung von completes the education of young scientists at IPK. Nachwuchswissenschaftlern am IPK komplettiert. In the past two years more than 10 workshops offering social In den vergangenen zwei Jahren wurden über 10 Arbeitssemi- and key competencies have been organised, usually attracting nare zur Vermittlung von Sozial- und Schlüsselkompetenzen a stable number of participants. In order to provide relevant angeboten, die sich zumeist reger Beteiligung erfreuten. Um workshops, the special demands and needs of students are ein möglichst optimales Workshop-Angebot aufstellen zu kön- collected via short surveys every year. An evaluation after the nen, werden die Nachfrage und die Bedürfnisse der Doktoran- completion of the event ensures the implementation meets den jährlich neu ermittelt. Eine anschließende Evaluierung der particular expectations and quality requirements. In addition, Veranstaltungen soll sicherstellen, dass Erwartungen und Qua- further specific requests can be integrated into the programme litätsansprüche erfüllt werden. Zudem werden spezielle Anfra- at any time. For example, this year a statistics seminar was of- gen jederzeit mit in das Programm aufgenommen. So konnte fered in cooperation with Dr. Armin Meister, and a seminar to 2011 in Kooperation mit Dr. Armin Meister beispielsweise ein gain a ‘Good Laboratory Practice’ (GLP) certificate was held in Statistikkurs angeboten werden oder, wie in 2010, ein Seminar 2010. zur Erlangung eines Zertifikats zur ‚Good Laboratory Practice‘ In cooperation with BIO Mitteldeutschland GmbH study trips (GLP). to larger firms were initiated last year and are now regularly of- In Zusammenarbeit mit der BIO Mitteldeutschland GmbH wer- fered. The PhD students have the opportunity to talk directly den seit letztem Jahr nun regelmäßig Exkursionen in die Indu- to human resources managers and get interesting insights into strie angeboten. Damit ist es den Doktoranden möglich, direkt the businesses of companies such as Merz Pharmaceuticals, ins Gespräch mit Personalverantwortlichen von Firmen wie Bioplanta and VITA 34 AG. Furthermore, companies on-campus Merz Pharmaceuticals, Bioplanta, VITA 34 AG etc. zu kommen are of particular interest and were invited by the PSB. Therefore und einen Einblick in Firmenabläufe zu erhalten. Zudem sind SunGene GmbH and TraitGenetics GmbH gave valuable over- aber auch die Unternehmen in direkter Nachbarschaft zum IPK views about their concepts and strategies and it is planned that für das PSB von Interesse. Einladungen zu Firmenpräsentatio- other companies will be invited in the future. The seminar and nen wurden von SunGene GmbH und TraitGenetics GmbH gern education programme makes an important contribution to the angenommen und sind auch zukünftig mit weiteren Firmen PhD qualification atI PK. The study trips to enterprises are espe- bereits in Planung. Das so erstellte Seminar- und Exkursions- cially valuable in enabling the students to get to know alterna- angebot leistet damit einen wichtigen Beitrag zur Doktoran- tive career options and to see a broader picture. denausbildung am IPK. Dabei erlauben es insbesondere die In- Over the past years the PSB has integrated itself into the semi- dustrieexkursionen, einen Blick über den Tellerrand zu werfen, nar programme of IPK and hosted prestigious international sci- um auf diesem Wege auch alternative Karrierewege kennen zu entists. In addition to six invited guest speakers, the renowned lernen. scientist Professor Sir David C. Baulcombe from Cambridge Des Weiteren hat sich das PSB mit seinen erfolgreichen Einla- University was welcomed at IPK. With those seminars PSB pro- dungen von international bedeutenden Wissenschaftler/-innen motes the contact between PhD students and the scientific mittlerweile als fester Bestandteil des Seminarprogramms am community beyond IPK. IPK etabliert. Neben sechs eingeladenen Gastrednern, konnte beispielsweise der bekannte Professor Sir David C. Baulcombe von der University of Cambridge dieses Jahr am IPK begrüßt werden. Damit fördert das PSB den Kontakt zwischen Dokto- randen und der wissenschaftlichen Gemeinschaft über das IPK hinaus.

155 Das Doktorandenprogramm/The PhD Programme

Abb. 52 Die ersten Preisträger des „Beagle Awards“: Dr. Manuela Na- gel (l.; Ag Ressourcengenetik und Reproduktion), Dr. Nicolai M. Nürk (Mitte; Ag Experimentelle Taxonomie) und Dr. Astrid Junker (r.; Ag Pflanzenbioinformatik; Foto: R. Schnee/IPK Gatersleben). Fig. 52 The first award winners of the newly established ‘Beagle Award’: Dr. Manuela Nagel (Resources Genetics and Repro- duction group), Dr. Nicolai M. Nürk (Experimental Taxonomy group) and Dr. Astrid Junker (Plant Bioinformatics group). (left to right; Photo: R. Schnee/IPK Gatersleben).

Eine weitere Aktivität des PSB ist die Organisation der Plant The PSB is also organiser of the Plant Science Student Confer- Science Student Conference (PSSC), welche 2012 zum achten ence (PSSC) which will be held for the 8th time in 2012. This Mal stattfinden wird. DieseK onferenz wird von Doktoranden für conference is organised by PhD students for PhD students Doktoranden organisiert und findet jährlich im Wechsel mit dem and is taking place at IPK and IPB Halle biannually. Between 70 IPB Halle statt. Zwischen 70 und 80 Nachwuchswissenschaftler and 80 young scientists from institutes of the Leibniz Associa- aus Instituten der Leibniz-Gemeinschaft, der Max-Planck-Gesell- tion, the Max Planck Society and the Julius Kühn Institutes can schaft und der Julius Kühn-Institute kommen dabei regelmäßig present their research as oral and poster presentations to other zusammen und haben die Möglichkeit, ihren Promotionskolle- PhD students. Outstanding performances are finally awarded gen ihre Forschungsarbeiten in Form von Vorträgen und Poster- prizes by an experienced jury consisting of at least five estab- präsentationen vorzustellen. Herausragende Beiträge werden lished scientists. dabei von einer kompetenten Jury, bestehend aus mindestens In 2011 the ‘Beagle Award’ was awarded for the first time. fünf etablierten Wissenschaftlern, ausgezeichnet. This IPK-wide prize was established by the PSB and aims to Erstmals konnte in diesem Jahr der sogenannte “Beagle recognise the scientific achievements and social dedication of Award” verliehen werden. Dieser institutsinterne Preis wurde PhD Students and will be announced every year. Astrid Jun- nach mehrjähriger Planung vom PSB am IPK etabliert und hat ker, Manuela Nagel and Nicolai Nürk received the award after zum Ziel, sowohl die wissenschaftlichen Leistungen als auch die an evaluation by an eight-member jury that consisted of IPK gemeinschaftlich-sozialen Fähigkeiten und das Engagement scientists, PSB members and non-scientific IPK staff. von Doktoranden, die am IPK tätig sind, zu würdigen und soll jährlich ausgeschrieben werden. In diesem Jahr wurden Astrid An important step for the improvement of the PhD develop- Junker, Manuela Nagel und Nicolai Nürk von der achtköpfigen ment programme is the introduction of a seminar series that Jury, die sich aus IPK-Wissenschaftlern, PSB-Mitgliedern und ei- gives information about the main research topics of the insti- nem nichtwissenschaftlichen IPK-Mitarbeiter zusammensetzte, tute to be presented by IPK scientists. In this respect the PSB geehrt. has developed a detailed concept for the establishment of a Als wichtiger Punkt zum Ausbau des promotionsbegleitenden course programme at IPK that was discussed thoroughly with Programms des PSB wird die Einführung einer wissenschaftli- responsible persons from the administration and the Board of chen Seminarreihe betrachtet, in der die Forschungsschwer- Directors. The main aim of these intensive efforts is the forma- punkte des IPK von IPK-Wissenschaftlern an die Doktoranden tion of a comprehensively structured ‘Graduate School’ which vermittelt werden. In diesem Zusammenhang wurde bereits zu integrates the programme organised by the PSB as an obliga- Beginn letzten Jahres innerhalb des PSBs ein detailliertes Kon- tory part of the PhD qualification at IPK. zept zur Etablierung eines Doktorandenprogramms erarbeitet und intensiv mit Verwaltungs- und Direktoriumsmitgliedern diskutiert. Ziel dieser Anstrengungen ist es, ein Doktoranden- Nicole Schmid, November 2011 programm in Form einer umfassend strukturierten “Graduate School” aufzubauen, in der das promotionsbegleitende Pro- gramm des PSB als obligatorischer Teil der Doktorandenausbil- dung fest integriert ist.

Nicole Schmid, November 2011

156 Kolloquien und Seminare/ Colloquia and Seminars

Gatersleben Lectures 2011

8. Februar 2011 2010 Prof. Dr. J. Ma, Department of Cellular and Molecular Medicine, St. George´s Hospital Medical, London, UK: Manufacturing re- 16. Februar 2010 combinant pharmaceuticals in plants – developing a pipeline Prof. Dr. C. Jung, Christian-Albrechts-Universität zu Kiel, Institut of products. für Pflanzenbau und Pflanzenzüchtung, Kiel, Germany: Bolting time control in sugar beet and applications in plant breeding. 14. März 2011 Prof. Dr. Q. Zhang, National Key Laboratory of Crop Genetic 24. März 2010 Improvement and National Center of Plant Gene Research Prof. Dr. M. D. McMullen, University of Missouri, Division of Plant Huazhong Agricultural University, Wuhan, China: Genetic and Sciences, Columbia, USA: Using genetic diversity to understand molecular characterization of grain size in rice. phenotypic variation in maize. 3. Mai 2011 29. April 2010 Prof. Dr. S. Schuster, Friedrich-Schiller-Universität, Jena, Germa- Prof. Dr. M. Yano, QTL Genomics Research Center, National In- ny: Biochemical examples of application of elementary-modes stitute of Agrobiological Sciences, Tsukuba, Japan: Genomics- analysis to plant metabolism. assisted allele mining and its integration to breeding in rice. 17. Mai 2011 11. Mai 2010 Prof. Dr. C.-C. Schön, Lehrstuhl für Pflanzenzüchtung, Techni- Prof. Dr. G. Coupland, Max-Planck-Institut für Pflanzenzüch- sche Universität, München, Germany: Genome-based predic- tungsforschung, Abt. Entwicklungsbiologie der Pflanzen, Köln, tion of testcross values in maize. Germany: Seasonal flowering in annual and perennial plants. 4. Juli 2011 17. August 2010 Prof. Dr. M. B. Dickman, Texas A & M University, USA: Death be Prof. Dr. U. Bonas, Martin-Luther-Universität Halle-Wittenberg, not proud: Modulation of programmed cell death for fungal Institut für Biologie, Abteilung Pflanzengenetik, Halle/S., Ger- disease development/stress tolerance in plants. many: How a bacterial pathogen effector manipulates plant transcription. 5. Juli 2011 Prof. Dr. P. Shaw, John Innes Centre, Norwich, UK: Chromatin dy- 21. Oktober 2010 namics and development. Prof. Dr. S. Abel, Leibniz-Institut für Pflanzenbiochemie, Abt. Molekulare Signalverarbeitung, Halle/S., Germany: Phosphate 8. Juli 2011 sensing in root development. Prof. Dr. T. Fujiwara, Department of Applied Biological Chem- istry, Graduate School of Agricultural and Life Sciences, Uni- 16. November 2010 versity of Tokyo Yayoi, Japan: Specificity and universality of the Prof. Dr. W. Martin, Heinrich-Heine-Universität Düsseldorf, Insti- relationship between plants and boron: Molecular mechanisms tut für Botanik, Düsseldorf, Germany: Hydrothermal vents and of acclimation to low and high boron conditions. the origin of life, what is the connection? 16. September 2011 2. Dezember 2010 Prof. Sir D. C. Baulcombe, University of Cambridge, Department Prof. Dr. C. Franklin, University of Birmingham, School of Biolog- of Plant Sciences, Cambridge, UK: Mobile short RNAs, epigenet- ical Sciences, Molecular and Cell Biology, Birmingham, UK: Con- ics and hybrid phenotypes in plants. trol of inter-homolog recombination in Arabidopsis meiosis. 26. September 2011 6. Dezember 2010 Prof. Dr. J. Messing, Waksman Institute of Microbiology, Rutgers Prof. Dr. W. B. Frommer, Stanford School of Medicine, Carnegie University, New Jersey, USA: Quality Protein Maize. Institution for Science, Department of Plant Biology, Stanford, USA: FRET sugar sensors uncover a novel class of sugar transporters important for nutrient supply to pathogens.

157 Kolloquien und Seminare/Colloquia and Seminars

11. Oktober 2011 26. Mai 2010 Prof. Dr. P. Christou, Universitat de Lleida, Spain: Multi-gene and Dr. M. Pelc, Warsaw University of Life Sciences, Department of multi-pathway engineering for the nutritional improvement of Vegetable and Medicinal Plants, Warsaw, Poland: Medicinal and food crops. aromatic plants in Poland and approaches to preserve their germplasm. 15. November 2011 Prof. Dr. R. Bock, Max-Planck-Institut für Molekulare Pflanzen- 7. Juli 2010 physiologie, Golm, Germany: Genes gone wild: experimental J. Walter, Helmholtz-Zentrum für Umweltforschung GmbH – UFZ, genome evolution in plants. Department Naturschutzforschung, Leipzig, Germany: Do plants remember drought? Evidence for drought-memory in grasses. 6. Dezember 2011 Prof. Dr. U. Paszkowski, University of Lausanne, Switzerland: The 11. August 2010 art and design of harmony: novel AM-factors from cereals. Dr. K. Zaynali Nezhad, Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) Gatersleben, Abteilung Gen- bank, Gatersleben, Germany: Genetic linkage map construction and identification of quantitative trait loci (QTLs) determining Vavilov- und PGRC-Seminare/ post-anthesis drought tolerance in bread wheat. Vavilov and PGRC Seminars 15. September 2010 Dr. P. Römer, GHG Saaten GmbH, Aschersleben, Germany: Eva- 2010 luierung von verschiedenen Herkünften sowie von Zuchtma- terial von Basilikum (Ocimum basilicum) im Hinblick auf ihre 9. März 2010 Kältetoleranz. M. Rosenhauer, Hochschule für Technik und Wirtschaft Dres- den, Fakultät Landbau/Landespflege, Dresden, Germany: Ge- 20. Oktober 2010 netische Untersuchungen des Merkmals Langlebigkeit von Prof. Dr. F. Corbineau, University Pierre et Marie Curie, Faculty of Saatgut an der Spezies Brassica napus L. Medicine, Paris, France: Seed deterioration and storage: Basic and applied aspects. 7. April 2010 Dr. N. Tikhenko, Institute St. Petersburg State University, Lab. 27. Oktober 2010 of Plant Genetics of Biological Research, St. Petersburg, Russia: Dr. S. P.C. Groot, Plant Research International B.V., Wageningen Postzygotic reproductive isolation systems in plants. Seed Centre, Wageningen, The Netherlands: The effect of oxygen on seed ageing. 13. April 2010 T. Struckmeyer, Julius Kühn-Institut, Bundesforschungsinstitut 3. November 2010 für Kulturpflanzen (JKI), Institut für Züchtungsforschung an Prof. M. Stein, Seed Bank, Evogene Ltd, Rehovot, Israel: Intro- gartenbaulichen Kulturen und Obst, Quedlinburg, Germany: duction to Evogene and seed bank activity. Petersilie (Petroselinum crispum) – Charakterisierung der Varia- bilität. 1. Dezember 2010 Dr. Johannes Ravn Jørgensen, Aarhus University, Faculty of Ag- 11. Mai 2010 ricultural Sciences, Department of Genetics and Biotechnology, E. Mattana, Ph D. Università degli Studi di Cagliari, Centro Con- Slagelse, Denmark: Use of non-destructive technologies to de- servazione Biodiversità, Cagliari, Italy: Sardinian Germplasm termine seed quality. Bank (BG-SAR): conservation strategies and research activities.

12. Mai 2010 2011 Dr. D. T. F. Endresen, Nordic Genetic Resources Centre (NordGen), Biodiversity Informatics, Alnarp, Sweden: Predictive association 9. Februar 2011 between trait data and eco-geographic data for barley landrac- Dr. E. Maul, Julius Kühn-Institut (JKI) Bundesforschungsinstitut es (Trait mining with eco-geographic data for identification of für Kulturpflanzen, Institut für Rebenzüchtung Geilweilerhof, trait properties useful for improvement of food crops). Siebeldingen, Germany: Die Domestikation der Rebe im Zeit- raffer. 20. Mai 2010 Dr. Y. Shavrukov, University of Adelaide, Australian Centre for 23. März 2011 Plant Functional Genomics, Adelaide, Australia: Nax3 – a novel T. Jinjikhadze, Ilia State University, National Botanical Garden of gene for sodium exclusion in barley. Mapping and identification: Georgia, Institute of Botany, Tbilisi, Georgia: Creation and use of Mystery and reality. the national inventory of wheat wild relatives (Aegilops L.).

158 31. März 2011 23. November 2011 Dr. habil. H.K. Parzies, Universiät Hohenheim, Institut für Pflan- Dr. M. Muminova, Tashkent Chemical-Technological Institute, zenzüchtung, Saatgutforschung und Populationsgenetik, Tashkent, Uzbekistan: Antioxidants in and cold tolerance of in Stuttgart, Germany: Marker assisted recurrent selection for in- vitro cultures for cryopreservation. creased outcrossing rates in barley and sorghum. 30. November 2011 27. April 2011 C.M. Richards, PhD, National Center for Genetic Resources Pres- A. Breuing, Leibniz-Universität Hannover, Institut für Zierpflan- ervation, Fort Collins, CO, USA: Eco-geographic differentiation zen- und Gehölzwissenschaften, Hannover, Germany: Einschät- in dwarf sunflower (Helianthus pumilus). zung der Kosteneffektivität verschiedener Strategien der Lang- zeiterhaltung von Knoblauch (Allium sativum L.) in der Genbank 30. November 2011 Gatersleben. I. Thormann, Bioversity International, Ex situ conservation and use project, Maccarese, Rome, Italy: Bioversity International 4. Mai 2011 projects to conserve and use agrobiodiversity. Dr. S. Mira, Universidad Politecnica de Madrid, Departamento de Biologia Vegetal E.U.I.T. Agricol, Madrid, Spain: Seed conser- 7. Dezember 2011 vation: physiological aspects and volatile production. Dr. I. Ferraz, The National Institute of Amazonian Research, Ma- naus, Brazil: Tropische Baumsamen und die Erfahrungen des 11. Mai 2011 brasilianischen Samennetzwerks aus dem Amazonasgebiet. Dr. W. Spielmeyer, CSIRO Plant Industries, Canberra, Australia: Durable rust resistance in wheat, are we there yet? 14. Dezember R. Joosen, Wageningen University, Department of Plant Physi- 23. Juni 2011 ology, Wageningen, The Netherlands: High-throughput ger- Dipl.-Biol. C. Scheriau, Ruprecht-Karls-Universität Heidelberg, mination analysis to determine the genetic landscape of seed Fakultät für Biowissenschaften, COS, Abteilung Biodiversität und performance. Pflanzensystematik, Heidelberg, Germany: The origin, centre of variation and evolutionary history of Hypericum perforatum L.

4. Juli 2011 Vavilov-Vortragsabende/Vavilov Lectures Dr. Y. Turuspekov, Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan: Barley research in Kazakhstan. 2010 15. Juli 2011 Dr. I. Kranner, Royal Botanical Gardens, Kew, Millenium Seed- 20. April 2010 bank Partnership, Richmond, UK: Was ist Stress? Konzepte und N. Nürk, Leibniz-Institut für Pflanzengenetik und Kulturpflan- Beispiele für invasive und nicht-invasive Diagnostik von Sa- zenforschung (IPK) Gatersleben, Abteilung Genbank, Gatersle- menqualität und -stress. ben, Germany: Im Regen, im August, in Japan – Impressionen einer Exkursion. 24. August 2011 Dr. A. Westphal, Julius Kühn-Institut (JKI) Bundesforschungsan- stalt für Kulturpflanzen, Institut für Pflanzenschutz in Ackerbau 2011 und Grünland, Münster, Germany: Damage potential of cyst nematodes on field crops. 20. April 2010 E. Willner, Leibniz-Institut für Pflanzengenetik und Kulturpflan- 14. September 2011 zenforschung (IPK) Außenstelle Nord, Abteilung Genbank, Mal- Dr. J. Buitink, INRA, Department of Molecular Seed Physiology, chow/Poel, Germany: Neuseeland – Eindrücke von einer Wan- Angers, France: Genetic analysis of seed-soluble oligosaccha- der- und Studienreise. rides in relation to seed vigour in Medicago truncatula. 19. Juli 2011 19. Oktober 2011 Dr. U. Lohwasser, Leibniz-Institut für Pflanzengenetik und Kul- Dr. M. Havrlentova, CVRV, Plant Production Research Centre turpflanzenforschung (IPK) Gatersleben, Abteilung Genbank, (PPRC), Piestany, Slovak Republic: Cereal β-D-glucan – a miracle Gatersleben, Germany: Jordanien – Land der Nabatäer. or a reality?

159 Kolloquien und Seminare/Colloquia and Seminars

Genetische Seminare/Genetics Seminars 18. Juni 2010 B. Yang, PhD, Beijing Genomics Institute (BGI), Shenzhen, China: Harvest the fruits of genomics. 2010 22. Juni 2010 26. Januar 2010 K. Dehesh, University of California, College of Biological Scienc- D. Wiechers, Leibniz-Universität Hannover, Hannover, Germany: es, Plant Biology Department, Davis, USA: Molecular mecha- Modelling the influence of canopy structure on light distribu- nisms regulating stress signalling networks in plants. tion and productivity of cucumber. 5. August 2010 1. Februar 2010 Dr. M. Wissuwa, Japan International Research Center for Agri- Dr. J. Kranigk, Bergdata Biometrics GmbH, Bonn, Germany: cultural Sciences (JIRCAS), Tsukuba, Japan: Abiotic stresses in Analysis of natural geometric structures – examples and appli- rice and genetic factors contributing to tolerance. cations. 20. September 2010 9. Februar 2010 Dr. N. Ohmido, Kobe University, Faculty of Human Develop- Dr. B. Steiner, Universität für Bodenkultur Wien, Department ment, Graduate School of Human Development and Environ- IFA-Tulln, Tulln, Austria: Gene expression analysis of near iso- ment, Kobe, Japan: Chromosome analysis based on the synteny genic wheat lines differing in two major resistance TQ L after of legume plants. Fusarium graminearum inoculation. 23. September 2010 17. März 2010 Prof. P. S. Soltis, University of Florida, Laboratory of Molecular Dr. Y. Markaki, Ludwig-Maximilians-Universität München, Systematics and Evolutionary Genetics, Gainesville, USA: Poly- Fakultät für Biologie, Ag Humangenetik, München, Germany: ploidy and plant evolution. Histone H3 threonine 3 phosphorylation is part of an editable histone modification pattern that marks and configures mitotic 22. Oktober 2010 chromatin. Dr. K. Vijverberg, Radboud University Nijmegen, Institute for Water and Wetland Research (IWWR), Department of Plant Ge- 12. April 2010 netics, Nijmegen, The Netherlands: Genetic fine-mapping of Prof. Dr. C. Cremer, Kirchhoff-Institut für Physik, Heidelberg, DIPLOSPOROUS in Taraxacum indicates a duplicated DIP-gene. Germany: Lightoptical imaging of cellular structures at molecular resolution. 25. November 2010 Dr. D. Dechyeva, Technische Universität Dresden, Lehrstuhl für 20. April 2010 Zell- und Molekularbiologie der Pflanzen, Dresden, Germany: Dr. U. Bechtold, University of Essex, Department of Biological Integration of genetic linkage and chromosomal maps of sugar Sciences, Colchester, UK: Over-expression of Arabidopsis Heat beet by high-resolution molecular cytogenetics. Shock Transcription Factor Alb increases drought tolerance, water productivity and basal disease resistance. 8. Dezember 2010 Dr. M. Trujillo, Julius-Maximillians-Universität Würzburg, Julius- 3. Mai 2010 von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Phar- O. Kirioukhova, PhD, University of Zurich, Institute of Plant Bi- mazeutische Biologie, Würzburg, Germany: Plant U-box type ology, Department of Plant Developmental Genetics, Zurich, E3-ubiquitin ligases as regulators of plant immunity. Switzerland: A novel Arabidopsis orthologue of Inner Centro- mere Protein is critical for reproductive development. 2011 31. Mai 2010 Dr. H. Vogel, Max-Planck-Institut für Chemische Ökologie, Ab- 25. Januar 2011 teilung Entomologie, Jena, Germany: Insect defenses in chemi- Dr. A.-C. Cosendai, Universität Wien, Fakultät für Lebenswissen- cal coevolution. schaften, Abteilung Botanische Systematik und Evolutionsfor- schung, Wien, Austria: What influences geographical parthe- 14. Juni 2010 nogenesis patterns in Ranunculus kuepferi: apomixis, genetic S. Andorf, Leibniz-Institut für Nutztierbiologie, Genetik und diversity and/or evolution? Biometrie, Ag Biomathematik und Bioinformatik, Dummerstorf, Germany: Integrating a molecular network hypothesis and QTL results for heterosis in Arabidopsis thaliana.

160 22. Februar 2011 30. Mai 2011 Dr. R. Ivanov, Universität des Saarlandes, Naturwissenschaftlich- Dr. J. Parker, National Research Council Canada, Plant Biotech- Technische Fakultät III, Abteilung für Molekulare Pflanzenbiolo- nology Institute, Saskatoon, Saskatchewan, Canada: The evolu- gie und Botanik, Saarbrücken, Germany: Insights into the role tion of farming, plant breeding, and biotechnology – the next of protein dynamics in Arabidopsis iron uptake. 30 years.

25. Februar 2011 1. Juni 2011 Prof. Dr. M. Lenhard, Universität Potsdam, Institut für Bioche- Dr. P. J. van Dijk, Keygene N.V., Wageningen, The Netherlands: mie und Biologie, Arbeitsgruppe Genetik, Potsdam, Germany: Molecular breeding to accelerate improvement of the Russian Genetic control and evolutionary modification of plant organ dandelion, a potential rubber crop. size. 5. August 2011 1. März 2011 Prof. Dr. R. K. Varshney, International Crops Research Institute Dr. B.-J. Shi, University of Adelaide, Australian Centre for Plant for the Semi-Arid Tropics, Patancheru, Hyderabad, India: Har- Functional Genomics, Adelaide, Australia: miRNA profiling in nessing the potential of genomics and germplasm diversity for barley. enhancing the legume productivity in Africa and Asia.

8. März 2011 18. August 2011 apl. Prof. Dr. J. C. Reif, Universität Hohenheim, Landesanstalten Dr. M. Stam, University of Amsterdam, Nuclear Organisation und Versuchsstationen, Landessaatzuchtanstalt, Stuttgart, Ger- Group, Amsterdam, The Netherlands: Gene regulation by epige- many: Modern biometrical models for QTL mapping of main netics and chromosomal interactions: paramutation in maize. and epistatic effects. 25. August 2011 10. März 2011 Prof. Dr. M. Guerra, Universidade Federal de Pernambuco, De- Dr. Ir. B. van der Zaal, Leiden University, Institute of Biology, Lei- partamento de Botânica, Recife, Brazil: Are chromosome band- den, The Netherlands: Zinc finger-based DNA binding proteins ing methods still useful nowadays? as tools for plant molecular genetics. 18. Oktober 2011 1. April 2011 D. Orzaez, University of Valencia, Instituto de Biología Molecular Prof. Dr. S. Renner, Ludwig-Maximilians-Universität München, y Celular de Plantas, Valencia, Spain: GoldenBraid: An iterative Fakultät für Biologie, Abteilung für Systematische Botanik und cloning system for standardized multigene assembly in plants. Mykologie, München, Germany: Sex Chromosomes in Land Plants. 30. November 2011 Prof. Dr. W. Traut, Universität Lübeck, Institut für Biologie, 12. April 2011 Lübeck, Germany: Evolution of sex chromosomes. Prof. Dr. C. Peterhänsel, Leibniz-Universität Hannover, Institut für Botanik: Photorespiration redesigned, Hannover, Germany: 24. November 2011 Synthetic bypass reactions and what they tell us about evolu- Prof. S. Abrams, National Research Council Canada, Plant Bio- tion of the pathway. technology Institute, Saskatoon, Saskatchewan, Canada: Plant hormone research toward crop improvement. 14. April 2011 Prof. Dr. T. Börner, Humboldt-Universität zu Berlin, Institut für 8. Dezember 2011 Biologie, Arbeitsgruppe Genetik, Berlin, Germany: Small ge- Dr. A. Manetto, baseclick GmbH, Tutzing, Germany: Click chem- nome and complex machinery: transcription of chloroplast ge- istry – a novel technology for efficient labeling of DNA and pro- nes. tein.

19. April 2011 Dr. D. van Damme, Ghent University, VIB Department of Plant Systems Biology, Ghent, Belgium: Aurora kinase 1 and 2 are required for correct cell division plane orientation during formative divisions in Arabidopsis.

4. Mai 2011 PD Dr. J. Boch, Martin-Luther-Universität Halle-Wittenberg, In- stitut für Biologie, Abteilung Genetik, Halle/S., Germany: TAL ef- fectors from Xanthomonas – a novel DNA-binding domain with programmable specificity.

161 Kolloquien und Seminare/Colloquia and Seminars

Zellbiologische Seminare/Cell Biology Seminars 27. Oktober 2010 Prof. Dr. C. Gaillardin, AgroParisTech/INRA, Department of Life Sciences and Health, Paris, France: Redirecting lipid metabo- 2010 lism in the oleaginous yeast Yarrowia lipolytica.

15. Januar 2010 18. November 2010 Prof. Dr. V. Passoth, Swedish University of Agricultural Sciences, Dr. C. Hermans, Université Libre de Bruxelles, Faculté des Department of Microbiology, Uppsala, Sweden: Non-conven- Sciences, Brussels, Belgium: Root morphological adaptation to tional yeasts for bioethanol production and beyond. nitrate availability in the model species Arabidopsis thaliana.

27. Januar 2010 6. Dezember 2010 Dr. L. Gugsa, Ethiopian Institute of Agricultural Research, EARO Dr. G. Sahni, Institute of Microbial Technology, Chadigarh, India: Holetta Research Centre, Holetta, Ethiopia: Biotechnology in Development of novel clot buster enzymes. the traditional Ethiopian cereal tef (Eragrostis tef). 14. Dezember 2010 2. Februar 2010 Dr. S. Tenzer, Johannes-Gutenberg-Universität Mainz, Universi- Prof. Dr. C. Stöhr, Ernst-Moritz-Arndt-Universität Greifswald, Ins- tätsmedizin, Institut für Immunologie, Mainz, Germany: Opti- titut für Botanik & Landschaftsökologie, Greifswald, Germany: mizing sample preparation and software for label-free quanti- Root specific plasma membrane proteins involved in nitrogen tative proteomics. assimilation and metabolism. 14. Dezember 2010 4. Juni 2010 Prof. Dr. H. Deising, Martin-Luther-Universität Halle-Wittenberg, Dr. J. Hüge, Dr. J. Kopka, Max-Planck-Institut für Molekulare Institut für Agrar- und Ernährungswissenschaften, Abteilung Pflanzenphysiologie, Angewandte Metabolom-Analyse/Wur- Pflanzenwissenschaften, Halle/S., Germany: Infection strategy zelmetabolismus, Golm, Germany: Metabolic profiling of - cy of the maize pathogen Colletotrichum graminicola. anobacteria with special emphasis towards inorganic carbon limitation of Synechocystis sp. PCC 6803. 2011 10. Juni 2010 Prof. J. Degenhardt, Martin-Luther-Universität Halle-Witten- 5. Januar 2011 berg, Institut für Pharmazie, Abteilung Pharmazeutische Bio- Dr. M. Pourkheirandish, National Institute of Agrobiological technologie, Halle/S., Germany: The roles of volatile terpenes in Sciences, Plant Genome Research Unit, Tsukuba Ibaraki, Japan: direct and indirect defenses of plants. Domestication related gene in barley.

15. September 2010 22. März 2011 Prof. Dr. R. Scheibe, Universität Osnabrück, Fakultät für Biologie S. Lingam, Universität Saarbrücken, Institut für Botanik, Saar- und Chemie, Botanischer Garten, Osnabrück, Germany: Role of brücken, Germany: Expression and stability of the transcription malate valves for redox-homeostasis in light and dark. factor FIT – Crosstalk between iron uptake, ethylene response and nitric oxide signalling. 29. September 2010 Dr. A. Svatos, Max-Planck-Institut für Chemische Ökologie, Mass 9. Mai 2011 Spectrometry/Proteomics, Jena, Germany: Looking through Prof. Dr. L. Anderson, University of Illinois at Chicago, Depart- mass spectrometric magnifying glass: distribution of metabo- ment of Biological Sciences, Chicago, USA: Enzyme-enzyme lites in natural samples. interaction in the plant cloroplast.

20. Oktober 2010 31. Mai 2011 Dr. V. Ordenes, Center for Advanced Studies on Arid Zones Prof. Dr. S. Schmidt, The University of Queensland, Faculty of (CEAZA), La Serena, Chile: BioaCu Initiative: From molecular Science, School of Biological Sciences, Brisbane, Australia: Ni- function to clean production. trogen stories from ecosystems to molecules.

27. Oktober 2010 23. Juni 2011 Dr. C. Neuvéglise, AgroParisTech/INRA, Department of Life Dr. F. Barriere, Université de Rennes 1, Institut des Sciences Sciences and Health, Paris, France: Exploratory genomics of Chimiques de Rennes, France: Enzymes and microbes as cata- oleaginous yeasts. lysts in fuel cells.

162 27. Juli 2011 11. August 2010 Dr. M. Wissuwa, Japan International Center for Agricultural A. S. Ghassemi Hosseini, Technische Universität Darmstadt, Sciences, Tsukuba, Japan: Phosphorus and Zinc efficiency in Fraunhofer-Institut für Graphische Datenverarbeitung IGD, rice: From QTLs to tolerance mechanisms and associated gene. Darmstadt, Germany: Bone mineral density (BMD) assessment with dual energy CT. 22. August 2011 Dr. K. Baronian, University of Canterbury, School of Biological 3. September 2010 Sciences, Microbiology, Christchurch, New Zealand: The Arxula Prof. I. Small, University of Western Australia, ARC Centre of Ex- microbial fuel cell; can it match bacterial microbial fuel per- cellence, Plant Energy Biology, Crawley, Australia: Perfectly pre- formance. pared RNA: getting transcripts ready for translation.

6. September 2011 23. September 2010 Prof. Dr. S. Baginsky, Martin-Luther-Universität Halle-Witten- Dr. J. Hollunder, Ghent University, VIB Department of Plant Sys- berg, Institut für Biochemie und Biotechnologie, Abteilung tems Biology, Ghent, Belgium: Module discovery – getting the Pflanzenbiochemie, Halle/S., Germany: Functional proteomics: ‘gold’ out of your data. A cornerstone in plant systems biology. 23. November 2010 2. November 2011 M. Hotze, Friedrich-Schiller-Universität Jena, Fakultät für Math- Dr. T. Boller, University of Basel, Institute of Botany, Basel, Swit- ematik und Informatik, Lehrstuhl Bioinformatik, Jena, Germany: zerland: Pattern recognition receptors in plant innate immunity In silico sequence optimization of synthetic genes and assign- and some surprises with mycorrhizal fungi. ment of the expression levels using transcription analysis in Candida albicans. 19. Dezember 2011 Dr. V. Ordenes Ortiz, Sciences & Biotechnology, Chile: Yeast- based technologies for biomining and bioremediation. 2011

13. Januar 2011 Dr. M. Strickert, Universität Siegen, Institut für Bildinformatik, Waterman-Seminare/Waterman Seminars Siegen, Germany: Partial generalized correlation and matrix correlation mapping for high-dimensional data and multispec- tral images. 2010 9. Februar 2011 23. Februar 2010 Dr. M. van Iersel, Maastricht University, BiGCaT Bioinformatics D. Albrecht, Leibniz-Institut für Naturstoff-Forschung und In- and Systems Biology Research Group, Maastricht, The Nether- fektionsbiologie e.V. ‑ Hans-Knöll-Institut (HKI) Jena, Abteilun- lands: Data integration with biological pathways. gen Systembiologie/Bioinformatik, Jena, Germany: OmniFung – Data Warehouse for integrating fungal omics data. 11. Februar 2011 Dr. A. C. Villéger, University of Manchester, School of Computer 13. April 2010 Science, Manchester, United Kingdom: Perspectives on the Vi- Dr. I. A. Paponov, Albert-Ludwigs-Universität Freiburg, Fakultät sualisation of Biological Systems: From biochemical models to für Biologie/Institut für Biologie II, Freiburg, Germany: Bioinfor- systems biology maps, and back. matics as a tool to reveal the mechanism of auxin action. 23. März 2011 12. Mai 2010 Dr. A. Beyer, Technische Universität Dresden, Biotechnologi- Dr. D. T. F. Endresen, Nordic Genetic Resources Centre (NordGen), sches Zentrum (BIOTEC), Dresden, Germany: Disentangling ge- Biodiversity Informatics, Alnarp, Sweden: Predictive association netic networks controlling complex traits. between trait data and eco-geographic data for barley landraces (Trait mining with eco-geographic data for identification of 26. Mai 2011 trait properties useful for improvement of food crops). Dr. S. Arvidsson, Max-Planck-Institut für Molekulare Pflanzen- physiologie, Arbeitsgruppe Bioinformatik, Potsdam-Golm, Ger- 3. Juni 2010 many: Phenotyping Arabidopsis thaliana with the LemnaTec Dipl.-Inf. R. Henkel, Graduiertenkolleg dIEM oSIRIS, Universi- Scanalyzer HTS system. tät Rostock, Fakultät für Informatik und Elektrotechnik (IEF), Institut für Informatik, Datenbank- und Informationssysteme, Rostock, Germany: Information retrieval for computational biological models.

163 Kolloquien und Seminare/Colloquia and Seminars

27. Mai 2011 7. Juli 2011 Prof. Dr.-Ing. R. Dachselt, Otto-von-Guericke-Universität Mag- Dr. A. W. Schreiber, The University of Adelaide, Australian Centre deburg, Fakultät für Informatik, Institut für Simulation und Gra- for Plant Functional Genomics (ACPFG), Bioinformatics Group, phik, Magdeburg, Germany: Natural ways of interacting with Adelaide, Australia: Assembling the transcriptome of bread information spaces. wheat.

27. Mai 2011 8. Juli 2011 Prof. Dr.-Ing. A. Nürnberger, Otto-von-Guericke-Universität Prof. Dr. B. Meyer, Monash University, Faculty of Information Magdeburg, Fakultät für Informatik, Institut für Technische und Technology, Centre for Research in Intelligent Systems (CRIS), Betriebliche Informationssysteme (ITI), Magdeburg, Germany: Clayton, Australia: Mavericks required – understanding collec- Context-based information exploration. tive decision making through diffusion modelling.

1. Juli 2011 12. September 2011 Dr.-Ing. S. Oeltze, Otto-von-Guericke-Universität Magdeburg, A.-L. Lamprecht, Technische Universität Dortmund, Fakultät für Fakultät für Informatik, Institut für Simulation und Graphik, Informatik, Dortmund, Germany: Semantics-supported design Magdeburg, Germany: Interactive, graph-based visual analysis of bioinformatics workflows in Bio-jETI. of protein colocalization studies in toponomics. 7. November 2011 J. Einloft, Johann-Wolfgang-Goethe-Universität Frankfurt am Main, Institut für Molekulare Biowissenschaften, Abteilung für Molekulare Zellbiologie der Pflanzen, Frankfurt am Main, Ger- many: MonaLisa ‑ visualization of Petri nets and more.

164 Vorträge und Poster/ Lectures and Posters

Eingeladene Vorträge auf internationalen in vitro culture – state of the art as conservation strat- Tagungen (Auswahl)/Invited Lectures egy for genebanks. – International Horticulture Con- at International Conferences (Selection) gress IHC, Symposium on New Tools for Plant Genetic Resources, Lisbon/Portugal, 22.-29.08.2010. V12. Kilian, B., S.S. Ja k o b , H. Öz k a n , S. Sh a a f , F.A. Ko n o v a l o v , R.K. 2010 Pa s a m , R. Sh a r m a , S. Hü b n e r , F. Sa l a m i n i , A. Gr a n e r , M. v o n Ko- r f f & G. Co u p l a n d (vorgetragen von Kilian, B.): Comparing V1. Ag a r w a l , R., M. Me l z e r & J.K. Sainis (vorgetragen von Ag a r - genetic diversity within a crop and its wild progenitor: w a l , R.): High Pressure Freezing of cyanobacteria for im- a case study for barley. – ECPGR Symposium “Towards munolocalisation studies. – International Conference the Establishment of Genetic Reserves for Crop Wild on Advances in Electron Microscopy and Related Tech- Relatives and Landraces in Europe”, Funchal/Madeira, niques & XXXI Annual Meeting of EMSI (EMSI-2010), 13.-16.09.2010. Mumbai/India, 08.-10.03.2010. V13. Kr a c h , C. & B. H. Ju n k e r (vorgetragen von Kr a c h , C.): Sys- V2. Al t m a n n , T.: Analysis of Arabidopsis natural genetic varia- tems biology approaches to crop plant metabolism. tion and heterosis in biomass accumulation and metab- – Brazil-Deutschland Systems Biology Meeting, Ouro olism. – 10th Korean-German Joint Symposium, Jinju/ Preto/Brazil, 25.-29.04.2010. Korea, 27.-28.09.2010. V14. Ku m l e h n , J.: Genetic engineering in cereals – methods V3. Bö r n e r , A.: High quality long term conservation, repro- and applications. – Symposium and Training Course duction and utilisation of plant genetic resources at the on Climate Change – Challenge for Plant Breeding German ex situ genebank. – 2nd Workshop “Seed quality and the Biotech Response, Martonvásár/Hungary, 12.- in genetic resources conservation of cultivated plants”, 16.04.2010. Warsaw/Poland, 25.-26.05.2010. V15. Ku m l e h n , J., T. Gu s e , C. Ma r t h e , E. Gr ü t z e m a n n , C. Bo l l m a n n V4. Fl o r s c h ü t z , K., A. Sc h r ö t e r , M. Kö r n e r , S. Sc h m i e d e r , F. So n n - & G. He n s e l (vorgetragen von Ku m l e h n , J.): Haploid tech- t a g , U. Kl o t z b a c h & G. Ku n z e (vorgetragen von Fl o r s c h ü t z , nology and genetic engineering in rye. – EUCARPIA ‑ K.): Detection of phytopathogenic RNA-viruses. – 16th International Symposium on Rye Breeding & Genetics, International Conference on Renewable Resources and Minsk/Belarus, 29.06.-02.07.2010. Plant Biotechnology, NAROSSA®2010, Magdeburg, 07.- V16. Me l z e r , M. & D. Da g h m a (vorgetragen von Me l z e r , M.): 08.06.2010. High pressure freezing and microwave-assisted tissue V5. Gr a n e r , A.: Exploiting the diversity of crop plants: pres- processing for transmission electron microscopy in ent state and future challenges. – 2nd International Sym- plant research. – International Conference on Advances posium on Genomics of Plant Genetic Resources, Bolo- in Electron Microscopy and Related Techniques & XXXI gna/Italy, 24.-27.04.2010. Annual Meeting of EMSI (EMSI-2010), Mumbai/India, V6. Gr a n e r , A.: Toward genomics-driven breeding of barley (Hor- 08.-10.03.2010. deum vulgare): challenges and opportunities. – 5th EPSO V17. Me y e r , R.C., D. Ri e w e , K. We i g e l t , B. Eb e r t , R. Sc h m i d t , J. Li s e c , Conference “Plants for Life”, Olos/Finland, 29.08.-02.09.2010. M. St e i n f a t h & T. Al t m a n n (vorgetragen von Me y e r , R.C.): V7. Gr a n e r , A.: Plant Genetic Resources: the challenge of Genetic and molecular analysis of early stage heterosis valorization. – 54th Annual Congress of the Italian So- for growth related traits in the model species Arabidop- ciety of Agricultural Genetics (SIGA), Matera/Italy, 27.- sis thaliana. – EUCARPIA Cereal Section Meeting, Cam- 30.09.2010. bridge/UK, 06.-08.04.2010. V8. Ho u b e n , A.: Mechanisms of uniparental genome elimi- V18. Me y e r , R.C., B. Eb e r t , B. Ku s t e r e r , J. Li s e c , D. Ri e w e , R. Sc h m i d t , nation in wide hybrids. – SEB (Society for Experimental M. St e i n f a t h , K. We i g e l t , A.E. Me l c h i n g e r , J. Se l b i g , L. Wi l l m i t z e r Biology) Annual Main Meeting, Prague/Czech Republic, & T. Al t m a n n (vorgetragen von Al t m a n n , T.): Molecular 30.06.-03.07.2010. and genetic analysis of biomass heterosis in Arabidopsis V9. Ho u b e n , A.: Histone phosphorylation – a dynamic affair. thaliana. – 21st International Conference on Arabidopsis – 7th Tri-National Arabidopsis Meeting (TNAM 2010), Research, Yokohama/Japan, 06.-10.06.2010. Salzburg/Austria, 15.-18.09.2010. V19. Me y e r , R.C., B. Eb e r t , B. Ku s t e r e r , J. Li s e c , D. Ri e w e , R. Sc h m i d t , V10. Ho u b e n , A.: Mechanisms of uniparental chromosome M. St e i n f a t h , K. We i g e l t , A.E. Me l c h i n g e r , J. Se l b i g , L. elimination. – Symposium EPS theme 4 “Genome Plas- Wi l l m i t z e r & T. Al t m a n n (vorgetragen von Al t m a n n , T.): Mo- ticity”, University of Wageningen, Wageningen/The lecular and genetic analysis of biomass and metabolite Netherlands, 10.12.2010. heterosis in Arabidopsis thaliana. – Brassica2010,17th V11. Ke l l e r , E.R.J., A. Se n u l a , C. Za n k e , M. Gr ü b e & A. Ka c z m a r c z y k Crucifer Genetics Workshop, Saskatoon/Canada, 05.- (vorgetragen von Ke l l e r , E. R. J.): Cryopreservation and 09.09.2010.

165 Vorträge und Poster/Lectures and Posters

V20. Na g e l , M.: Genetics and physiology of seed longev- V34. Wo b u s , A.M.: Stem cell research in Germany with specific ity. – 2nd Workshop “Seed quality in genetic resources consideration of human embryonic stem cells. – Confer- conservation of cultivated plants”, Warsaw/Poland, 25.- ence “Differing routes towards stem cell research: Germany 26.05.2010. and Italy”, University of Trento/Italy, 21.-22.09.2010. V21. Ra j a n i k a n t , C., M. Me l z e r & J.K. Sainis (vorgetragen von Ra- j a n i k a n t , C.): Visualizing DNA and DNA protein interactions of rice recombinase using TEM. – International Confer- 2011 ence on Advances in Electron Microscopy and Related Techniques & XXXI Annual Meeting of EMSI (EMSI-2010), V1. Al t m a n n , T.: Molecular and genetic analysis of biomass- and Mumbai/India, 08.-10.03.2010. metabolite-heterosis in Arabidopsis and maize. – European V22. Sc h u b e r t , I.: DNA damage and its chromosomal end- Plant Breeding Academy, Gatersleben, 20.06.2011. points in plants. – Plant and Animal Genome XVIII. Con- V2. Al t m a n n , T.: Molecular and genetic analysis of biomass- and ference, San Diego/USA, 09.-13.01.2010. metabolite-heterosis in Arabidopsis and maize. – Botani- V23. Sc h u b e r t , I.: Interphase chromosome arrangement is kertagung 2011 “Diversity makes the Difference”, Berlin, dynamic and important for homologous recombina- 18.-23.09.2011. tion repair. – 2nd International Conference on Plant DNA V3. Al t m a n n , T.: Molecular genetics of natural variation in plant Repair and Recombination 2010, Asilomar Conference growth and metabolism. – Banbury Meeting “Genotype Center, Pacific Grove, California/USA, 02.-05.03.2010. to phenotype: Deriving biological knowledge from large V24. Sc h w e i z e r , P.: Converging evidence for genes of basal genomic datasets”, Cold Spring Harbor Laboratory, New defense in barley. – 2nd International Symposium on York/USA, 16.-19.10.2011. Genomics of Plant Genetic Resources, Bologna/Italy, V4. Al t m a n n , T.: Molecular and genetic analysis of biomass- and 24.-27.04.2010. metabolite-heterosis in Arabidopsis. – Meeting “Epigenet- V25. Sc h w e i z e r , P.: Genes and loci for quantitative disease re- ics and Heterosis”, Black Mountain (Brisbane)/Australia 04.- sistance in barley. – 4th International Workshop Rauis- 07.12.2011. chholzhausen, Schloss Rauischholzhausen, Gießen, V5. Bo r i s j u k , L.: Integration of MRI and metabolic modelling to 01.10.2010. study spatial arrangement of storage in developing seed. V26. St e i n , N.: Physical mapping and sequencing of the bar- – BIT’s 1st Annual World Congress of Agricultural Biotech- ley genome: IBSC and the GABI program. – Plant and nology (WCAB-2011), Changchun/China, 28.-30.10.2011. Animal Genome XVIII. Conference, San Diego/USA, 09.- V6. Bö r n e r , A.: Plant genetic resources – molecular tools for 13.01.2010. characterization and utilization. Genomics of genebanks V27. St e i n , N.: Barley genome: maps & sequence. – EUCARPIA workshop. – Plant and Animal Genome XIX. Conference, Cereal Section Meeting, Cambridge/UK, 06.-08.04.2010. San Diego/USA, 15.-19.01.2011. V28. St e i n , N.: How to sequence a 5 Gigabase genome – a V7. Bö r n e r , A.: Plant genetic resources for food and agricul- case study of barley (Hordeum vulgare). – 1st EMEA GS ture (PGRFA) – conservation and utilisation. – Symposium FLX User Meeting, Athens/Greece, 15.-17.06.2010. on Genomics and Biodiversity, Hyderabad/India, 23.- V29. St e u e r n a g e l , B.: Utilizing next generation sequencing 25.02.2011. to analyze the complex genome of barley. – Bio IT V8. Bö r n e r , A., E.K. Kh l e s t k i n a , S. Ch e b o t a r , M. Na g e l , M.A. Re h m a n World Europe Conference & EXPO 2010, Hannover, 05.- Ar i f , K. Ne u m a n n , B. Ko b i l j s k i , U. Lo h w a s s e r & M S Rö d e r (vorge- 07.10.2010. tragen von Bö r n e r , A.): Maintenance and exploitation of ge- V30. v o n Wi r é n , N.: Morphological responses of Arabidopsis netic resources for future plant breeding. – Agrisafe Final roots to localized iron supply. – 15th International Sym- Conference “Climate Change: Challenge for Training of Ap- posium on Iron Nutrition and Interactions in Plants, Bu- plied Plant Scientists“, Budapest/Hungary, 21.-23.03.2011. dapest/Hungary, 26.-30.06.2010. V9. Co n r a d , U.: Production of vaccines and therapeutic anti- V31. v o n Wi r é n , N.: Identification of genes and mechanisms in- bodies for veterinary applications in transgenic plants: volved in nutrient acquisition and homeostasis. – XVII Con- an overview. – International Conference “Plant Transfor- ference of the Federation of European Societies of Plant mation Technologies II”, Vienna/Austria, 19.-22.02.2011. Biology (FESPB 2010), Valencia/Spain, 04.-09.07.2010. V10. Fl o r s c h ü t z , K.: On-Chip-Nachweis von phytopathogenen V32. v o n Wi r é n , N.: Localized ammonium supply increases lat- RNA-Viren und miRNA mittels Oberflächenplasmonen- eral root branching. – 1st International Symposium on resonanz. – fms-Sondersession 2011 “Sensorforschung the Nitrogen Nutrition of Plants (Nitrogen2010), Inuy- für Medizin und Technik”, Nürnberg, 09.06.2011. ama International Sightseeing Center “FREUDE”, Aichi/ V11. Fu c h s , J., I. Le r m o n t o v a , V. Sc h u b e r t , A. Pe c i n k a , M.A. Ly- Japan, 26.-30.07.2010. s a k , G. Jo v t c h e v & I Sc h u b e r t (vorgetragen von Fu c h s , J.): V33. Wo b u s , A.M.: Pancreatic differentiation of mouse embry- Flow-sorted nuclei are valuable subjects to investigate onic stem cells and the potential of Sox17-positive cells. structural and functional aspects of nuclear architec- – EMBO Workshop “Disease, development and stem cells ture in plants. – 6th Conference on Analytical Cytometry, in the pancreas”, Stockholm/Sweden, 14.-16.06.2010. Prague/Czech Republic, 08.-11.10.2011.

166 V12. Gi l s , M.: Pollination control systems for hybrid wheat V25. Ke l l e r , E.R.J., A. Se n u l a , C. Za n k e & M. Gr ü b e (vorgetragen breeding. – International Conference “Plants & People”, von Ke l l e r , E.R.J.): Genebank management of three Universität Potsdam, 06.09.2011. crops, potato, garlic and mint, and integration of cryo- V13. Gr a n e r , A.: Challenges and opportunities regarding the preservation in a complex collection. – International genomics-driven exploitation of genetic diversity in Conference on Cryopreservation of Horticultural Crops barley. – 15th Annual ADNAT Convention, International in China, Northwest A & F University, Yangling, Shaanxi, Symposium on Genomics and Biodiversity, Hyderabad/ China, 28.-30.06.2011. India, 23.-25.02.2011. V26. Ke l l e r , E.R.J., B. Pa n i s & F. En g e l m a n n (vorgetragen von V14. Gr a n e r , A.: Disentangling the barley genome – a com- Ke l l e r , E.R.J.): In vitro storage and cryopreservation as munity effort. – North American Barley Researchers substantial complements in concerted actions to better Workshop, Corvallis/USA, 06.-08.06.2011. maintain and use crop germplasm. – 7th International V15. Gr a n e r , A.: Sequence based utilization of plant genetic Symposium on In Vitro Culture and Horticultural Breed- resources of barley: opportunities and constraints. ing, Biotechnological Advances in In Vitro Horticultural – Indo-German Symposium on Plant Biology, Indian Breeding, Ghent/Belgium, 18.-22.09.2011. National Science Academy (INSA), New Delhi/India, 18.- V27. Kl u k a s , C.: Integrated visualization and analysis of *om- 20.10.2011. ics data, networks and images. – Institute of Cytology V16. Gr a n e r , A.: Genomics assisted valorization of plant genetic and Genetics SB RAS, Novosibirsk/Russia, 19.04.2011. resources of barley: Opportunities and constraints. – 25th V28. Kn ü p f f e r , H.: Rye genetic resources in the world’s gen- Colloquium “Crop Plants: Biodiversity & Genomics” of the ebanks. – International Conference “More Attention to Research Center of Biotechnology and Plant Breeding, Rye”, Tartu/Estonia, 06.-08.10.2011. Universität Hohenheim, 16.-17.11.2011. V29. Ku n z e , G.: On-Chip-Nachweis von phytopathogenen RNA- V17. Ha r t m a n n , A., T. Cz a u d e r n a , R. Ho f f m a n n , C. Kl u k a s , T. Al t - Viren mittels Oberflächenplasmonresonanz. – Sitzung m a n n & F. Sc h r e i b e r (vorgetragen von St e i n , N.): High- des fms/ProcessNet-Gemeinschafts-Ausschusses “Senso- throughput phenotyping in barley – the IPK plant ren und Sensorsysteme”, Frankfurt am Main, 27.01.2011. phenomics facilities. – Plant and Animal Genome XIX. V30. Ku n z e , G.: Arxula adeninivorans – a valuable tool for Conference, San Diego/USA, 15.-19.01.2011. characterization of metabolic pathways in eukaryotes, V18. He n s e l , G., A. Hi m m e l b a c h , D. No w a r a , C. Ka s t n e r , J. Ri e c h e n , producer of recombinant proteins and microbial com- D. Do u c h k o v , P. Sc h w e i z e r & J. Ku m l e h n (vorgetragen von pound for biosensors. – Institute of Cell Biology, NAS He n s e l , G.): A toolbox for the study of plant-pathogen of Ukraine, Department of Molecular Genetics and Bio- interactions in cereals. – AgriGenomics Congress, Ham- technology, Lviv/Ukraine, 15.02.2011. burg, 30.06.-01.07.2011. V31. Ku n z e , G.: Arxula adeninivorans – a valuable tool for basic V19. Ja k o b , S.S., C. He i b l , D. Rö d d e r & F. R. Bl a t t n e r (vorgetragen research and biotechnological application. – YNC 2011. von Ja k o b , S.S.): Population demography influences cli- 1st International Symposium on Nonconventional Yeast matic niche evolution: evidence from diploid American in Postgenomic Era, Lviv/Ukraine, 11.-14.09.2011. Hordeum species (Poaceae). – BioSystematics, Berlin, V32. Li p p m a n n , R., K. Wi t z e l , S. Ka s p a r , A. Ma t r o s & H. P. Mo c k 21.-27.02.2011. (vorgetragen von Mo c k , H.-P.): Plant proteomics to sup- V20. Ju n k e r , B.H.: Quantification of intracellular metabolic port breeding and biotechnological applications. – 3rd fluxes in developing plant seeds. – Workshop “Systems International Symposium “Frontiers in Agriculture Pro- Biology: Between Science and Application”, Frankfurt teome Research”, Tsukuba/Japan, 08.-10.11.2011. am Main, 18.-19.01.2011. V33. Ma t r o s , A.: Application of MALDI-MS imaging for spatial V21. Ju n k e r , B.H.: Central metabolic fluxes in seeds of the le- protein and metabolite profiling during barley grain devel- gume plant family. – JCB Workshop “Nucleotides – Net- opment. – Bruker Imaging Meeting, Bremen, 27.01.2011. works – Novelties. Understanding Evolution Beyond V34. Mo c k , H.-P.: Advanced techniques in mass spectrometry Darwin and Haeckel”, Jena, 28.-29.03.2011. and their potential to provide novel insights into plant V22. Ju n k e r , B.H.: Metabolic flux analysis in plant seeds – at seed biology. – 6th European Symposium on Enzymes in the interface between metabolomics and systems biol- Grain Processing, Carlsberg Research Center, Copenha- ogy. – Workshop “Trends in Metabolomics – Analytics gen/Denmark, 28.-30.11.2011. and Applications”, Frankfurt am Main, 19.-20.05.2011. V35. Pe u k e r t , M., S. Ka s p a r , A. Ma t r o s & H.-P. Mo c k (vorgetragen V23. Ju n k e r , B.H.: Quantification and simulation of intracellu- von Mo c k , H.-P.): Studying barley seed development by lar metabolic fluxes in plants. – International Fall Meet- spatially resolved mass spectrometrical analysis. – 3rd ing of the German Society for Biochemistry and Mo- International Symposium “Frontiers in Agriculture Pro- lecular Biology “Molecular Life Sciences”, Frankfurt am teome Research”, Tsukuba/Japan, 08.-10.11.2011. Main, 25.-28.09.2011. V36. Ro l l e t s c h e k , H.: Seed-specific elevation of non-symbiotic V24. Ju n k e r , B.H.: Towards high-throughput metabolic flux hemoglobin AtHb1: beneficial effects and underlying analysis in seeds of crop plants. – 7th Workshop “Molecu- molecular networks in Arabidopsis thaliana. – Interna- lar Interactions”, Berlin, 05.-07.10.2011. tional Conference for Plant Mitochondrial Biology 2011 (ICPMB), Hohenroda, 14.-19.05.2011.

167 Vorträge und Poster/Lectures and Posters

V37. Ro l l e t s c h e k , H.: Systems biology as tool for modeling Vorträge/Lectures and improvement of crop seeds. – BIT’s 1st Annual World Congress of Agricultural Biotechnology (WCAB-2011), Changchun/China, 28.-30.10.2011. 2010 V38. Sc h n u r b u s c h , T.: The developmental and genetic analysis of pre-flowering phases in barley and wheat. – Plant and V1. Al t m a n n , T.: WP1; Leaf Growth Phenotyping @MPI-MP/ Animal Genome XIX. Conference, Workshop “Genomics UP. – “AGRON-OMICS” Meeting, Bern, Konolfingen/Swit- of Plant Development and Signal Networks”, San Diego/ zerland, 03.-05.02.2010. USA, 15.-19.01.2011. V2. Al t m a n n , T.: Introduction: plant phenotyping at IPK. – Work- V39. Sc h u b e r t , I.: Recombination repair and mechanics of shop “Phenotyping and beyond”, Jülich, 18.-19.02.2010. chromosome and karyotype evolution. – Plant and V3. Al t m a n n , T.: Analysis of plant growth/biomass accu- Animal Genome XIX. Conference, San Diego/USA, 15.- mulation and metabolism in Arabidopsis and maize. 19.01.2011. – Workshop “Phenotyping and beyond”, Jülich, 18.- V40. Sc h u b e r t , I.: Interpretation of karyotype evolution should 19.02.2010. consider chromosome structural constraints. – XL Con- V4. Al t m a n n , T.: Analysis of Arabidopsis natural genetic varia- greso Argentino de Genética, III Simposio Latinoamericano tion and heterosis in biomass accumulation and metab- de Citogenética y Evolución I Jornadas Regionales SAG- olism. – CropDesign, Ghent/Belgium, 04.05.2010. NEA, Corrientes/Argentina, 18.-21.10.2011. V5. Al t m a n n , T.: Analysis of Arabidopsis natural genetic varia- V41. Sc h u b e r t , V., A. Be r r , A. Me i s t e r & I Sc h u b e r t (vorgetra- tion and heterosis in biomass accumulation and metab- gen von Sc h u b e r t , V.): Interphase chromatin organiza- olism. – Antrittsvorlesung, Martin-Luther-Universität tion in Arabidopsis. – 18th International Chromosome Halle-Wittenberg, Halle/S., 01.06.2010. Conference, University Place, Manchester/UK, 29.08.- V6. Al t m a n n , T.: Analysis of Arabidopsis natural genetic varia- 02.09.2011. tion and heterosis in biomass accumulation and me- V42. Sc h w e i z e r , P.: Genes and loci for quantitative disease re- tabolism. – CSHL – Pioneer/DuPont Plant Development sistance in barley. – 1st Congress of Cereal Biotechnol- and Phenomics Meeting, Banbury Center, Lloyd Harbor, ogy and Breeding, Szeged/Hungary, 24.-27.05.2011. New York/USA, 20.-21.09.2010. V43. Sc h w e i z e r , P.: Race-nonspecific powdery mildew resis- V7. Al t m a n n , T.: Moderne Methoden der Pflanzenforschung tance in barley: One trait – many genes. – Annual Meet- und -züchtung. – Dialogreihe „Innovationsfeld Pflanze“, ing of the Chinese Society for Plant Pathology, Yichang/ Magdeburg, 14.10.2010. China, 18.-24.08.2011. V8. Al t m a n n , T.: Die Suche nach dem Gold der Genbanken V44. Sc h w e i z e r , P.: Quantitative powdery mildew resistance – Neue Möglichkeiten der Erschließung und Nutzung in barley: One trait – many genes. – Botrytis/Sclerotinia genetischer Vielfalt. – GFP-Jahrestagung, Magdeburg, Post-Genome Workshop, Lyon/France, 15.-17.09.2011. 03.-04.11.2010. V45. Sc h w e i z e r , P.: Nichtwirtsresistenz – ein gangbarer Weg zu V9. Am i t e y e , S. & T. F. Sh a r b e l (vorgetragen von Am i t e y e , S.): Do dauerhafter Pathogenresistenz in Getreide? – Resistenz- microRNAs cause a loss of sex in Boechera? – 9th IMPRS tagung Fulda 2011 der GPZ, Fulda, 05.-06.12.2011. Symposium, Dornburg, 15.02.2010. V46. St e i n , N., R. Zh o u , T. Sc h m u t z e r , B. St e u e r n a g e l , U. Sc h o l z , V10. Am i t e y e , S. & T. F. Sh a r b e l (vorgetragen von Am i t e y e , S.): Do M.M. Ma r t i s , M. Se i d e l , K. Ma y e r , H. Si m k o v a , J. Do l e z e l , G. microRNAs cause a loss of sex in Boechera? – 6th Plant Ha s e n e y e r , E. Ba u e r , P. He d l e y , H. Liu & R. Wa u g h (vorgetra- Science Student Conference, IPK, Gatersleben, 15.- gen von St e i n , N.): Triticeae synteny revisited by barley 18.06.2010. chromosomal genomics. – Plant and Animal Genome V11. Ba k e r , S.M., H. Po s k a r & B. H. Ju n k e r (vorgetragen von XIX. Conference, San Diego/USA, 15.-19.01.2011. Ba k e r , S.M.): Unscented Kalman filter for estimation of V47. St e i n , N.: The impact of NGS technology on sequencing multiple parameters in kinetic models. – 7th Interna- the 5.1 gigabase barley genome. – Next Generation Se- tional Workshop on Computational Systems Biology quencing Day, Modena/Italy, 22.02.2011. (WCSB2010), Luxembourg, 16.-18.06.2010. V48. St e i n , N.: The International Barley Sequencing Consor- V12. Ba k e r , S.M. & B. H. Ju n k e r (vorgetragen von Ba k e r , S.M.): tium – when will the barley genome sequence be avail- Adaptive unscented Kalman filter for estimation of pa- able? – 9th Plant Genomics European Meeting (Plant rameters in kinetic metabolic models. – 4th International GEM), Istanbul/Turkey, 04.-07.05.2011. Conference on Computational Systems Biology (ISB V49. St e i n , N.: The barley genome – from virtual gene order to 2010), Suzhou/China, 09.-11.09.2010. a genetically anchored physical map. – 21st Internation- V13. Ba n a e i Mo g h a d d a m , A.M., M. Se i f e r t , F. Ro u d i e r , M. St r i c k e r t , al Triticeae Mapping Initiative (ITMI) Workshop, Mexico V. Co l o t , M.F. Me t t e & A. Ho u b e n (vorgetragen von Ba- City/Mexico, 05.-09.09.2011. n a e i Mo g h a d d a m , A. M.): Additive inheritance of histone V50. v o n Wi r é n , N.: Local Fe supply alters AUX-1 dependent modifications after intraspecific hybridization in Arabi- auxin accumulation and stimulates lateral root elonga- dopsis thaliana. – SEB (Society for Experimental Biology) tion in Arabidopsis plants. – Botanikertagung 2011 “Di- Annual Main Meeting, Prague/Czech Republic, 30.06.- versity makes the Difference”, Berlin, 18.-23.09.2011. 03.07.2010.

168 V14. Ba n a e i Mo g h a d d a m , A.M., M. Se i f e r t , F. Ro u d i e r , M. St r i c k e r t , V28. Bö r n e r , A.: Seed longevity – the focal point of ex situ V. Co l o t , M.F. Me t t e & A. Ho u b e n (vorgetragen von Me t t e , genebank collections. – Institutstag IPK, Gatersleben, M. F.): The stability of the epigenome in Arabidopsis 04.-05.10.2010. thaliana in response to intraspecific hybridization. – V29. Bö r n e r , A.: Pflanzengenetische Ressourcen –K leingärten 10th Gatersleben Research Conference 2010 (GRCX) in ihrer Bedeutung zur Erhaltung der Artenvielfalt. – 14. “Sequence-informed Crop Research”, Quedlinburg Pa- Forum Stadtgrün, Dresden, 25.11.2010. lais Salfeldt/Gatersleben (IPK), 22.-24.11.2010. V30. Br a s s a c , J. & F. R. Bl a t t n e r (vorgetragen von Br a s s a c , J.): V15. Bä u m l e i n , H.: An apospory-locus in Hypericum. – Univer- Analysis of phylogenetic relationships of Hordeum sität Regensburg, 03.02.2010. (Poaceae) polyploids. – 6th Plant Science Student Con- V16. Bä u m l e i n , H.: Identification and genetic analysis of the ference, IPK, Gatersleben, 15.-18.06.2010. APOSPORY locus in Hypericum perforatum. – EU COST V31. Br u c h m ü l l e r , A., J. Ku m l e h n , R. Sc h m i d t & M.F. Me t t e (vor- Meeting, Bristol/UK, 02.08.2010. getragen von Br u c h m ü l l e r, A.): RNA-directed RNA poly- V17. Bl a t t n e r , F.R.: Changes of ecological niche parameters in merases RDR5 is essential for transcript level-depen- Hordeum species during the Pleistocene. – Institutstag dent post-transcriptional gene silencing. – International IPK, Gatersleben, 04.-05.10.2010. Conference “Molecular Aspects of Plant Development”, V18. Bl a t t n e r , F.R.: Climate change and niche evolution in Vienna/Austria, 23.-26.02.2010. plants. – InWent refresher course, IPK, Gatersleben, 17.- V32. Co n r a d , U.: The Phytoantibody Group ‑ an overview. 20.05.2010. Jena, 26.03.2010. V19. Bö r n e r , A.: Pflanzengenetische Ressourcen – Ausgangs- V33. Co n r a d , U.: Production of spider silk derivatives in trans- material zur Züchtung auf abiotische Stresstoleranz bei genic plants: purification and characterization . – COST Getreide. – Pflanzengenetisches Kolloquium, Georg- Meeting, Vienna/Austria, 05.11.2010. August-Universität Göttingen, 14.01.2010. V34. Cz a u d e r n a , T.: SBGN-ED: editing, validating and translat- V20. Bö r n e r , A., M. Na g e l , M.A. Re h m a n Ar i f & U. Lo h w a s s e r (vor- ing of SBGN maps. – Workshop SBGN-5.5, Wittenberg, getragen von Bö r n e r , A.): Samenbanken – Ressourcen 21.-23.04.2010. für künftige Generationen. – 45. Vortragstagung der V35. Cz a u d e r n a , T.: ChromaViIns3D: Chromatogram visualisa- Deutschen Gesellschaft für Qualitätsforschung (DGQ), tion and inspection in 3D. – Task Force Metabolite Profil- Berlin, 22.-23.03.2010. ing, IPK, Gatersleben, 24.09.2010. V21. Bö r n e r , A. & M. Na g e l (vorgetragen von Bö r n e r , A.): Die V36. Cz a u d e r n a , T. & F. Sc h r e i b e r : SBGN-ED for editing, validat- internationale Bank für Pflanzensamen auf Spitzbergen ing, and translating of SBGN Maps and application ex- – Lagerung für die Ewigkeit? – Aachener Gesellschaft amples. – COMBINE 2010, Edinburgh, Scotland/UK, 06.- für Gartenkultur, Aachen, 25.03.2010. 09.10.2010. V22. Bö r n e r , A., B. Ko b i l j s k i & K. Ne u m a n n (vorgetragen von V37. Cz a u d e r n a , T. & A. Ha r t m a n n : Modelling, visualisation and Bö r n e r , A.): Association mapping of wheat germplasm data handling for Optimas. – Project Meeting Optimas, employing historical data. – 2nd International Sympo- Düsseldorf, 15.10.2010. sium on Genomics of Plant Genetic Resources, Bolo- V38. Da g h m a , D. & M. Me l z e r (vorgetragen von Da g h m a , D.): gna/Italy, 24.-27.04.2010. Embryogenesis of barley pollen – from live cell imaging V23. Bö r n e r , A.: 60 Jahre Evaluierung von Genbankmate- to ultrastructural analysis. – 6th Plant Science Student rial – unüberschaubares Datenmeer oder nutzbare Conference, IPK, Gatersleben, 15.-18.06.2010. Ressource. – GPZ-Tagung Geschichte der Pflanzenzüch- V39. De h m e r , K.J.: Research activities on the IPK forage collec- tung, Drübeck, 06.-07.05.2010. tions. – 10th ECPGR Forages Working Group Meeting, V24. Bö r n e r , A.: Molekulargenetische Ansätze zur Erhaltung Malchow (Poel), 28.-29.04.2010. und Nutzbarmachung pflanzengenetischer Ressourcen. V40. De h m e r , K.J.: Die Groß Lüsewitzer Kartoffel-Sortimente – Pflanzenzüchtung-Kolloquium, Martin-Luther-Univer- (GLKS) als Teilsammlung der IPK-Genbank. – Besichti- sität Halle-Wittenberg, Halle/S., 21.05.2010. gung des Kartoffelstandorts Groß Lüsewitz durch Mit- V25. Bö r n e r , A., K. Ne u m a n n & B. Ko b i l j s k i (vorgetragen von arbeiter einer Thüringer Kartoffelvertriebsgesellschaft; Bö r n e r , A.): Wheat genetic resources – how to exploit? Groß Lüsewitz, IPK Gatersleben, 18.06.2010. – 8th International Wheat Conference, St. Petersburg/ V41. De h m e r , K.J.: Die Groß Lüsewitzer Kartoffel-Sorti- Russia, 01.-04.06.2010. mente (GLKS) – kartoffelgenetische Ressourcen der V26. Bö r n e r , A., K. Ne u m a n n , S. Ko l l e r & M.S. Rö d e r (vorgetragen bundeszentralen IPK-Kulturpflanzenbank. – Mecklen- von Bö r n e r , A.): Assoziationsgenetische Studien in Wei- burger Kartoffeltag, Sanitz, 04.08.2010. zen. – GFP Sommertagung Getreide, IPK, Gatersleben, V42. De h m e r , K.J.: Analyse und Nutzung genetischer Res- 09.-10.06.2010. sourcen zur Erstellung neuer Diversität in Futter- und V27. Bö r n e r , A.: Die Kulturpflanzenbank in Gatersleben – Rasengräsern. – BLE-Innovationstage, Berlin, 06.- Grundlage für künftige Pflanzenforschung und Pflan- 07.10.2010. zenzüchtung. Biologische Vielfalt bei Pflanzen – Forsch- V43. De h m e r , K.J.: Diversity of potato genetic resources in re- ung in Sachsen-Anhalt. – Julius Kühn-Institut, Quedlin- spect to tuber quality traits. – Institutstag IPK, Gatersle- burg, 12.-13.08.2010. ben, 04.-05.10.2010.

169 Vorträge und Poster/Lectures and Posters

V44. Dobrovolskaya , O.B., P. Ma r t i n e k , O.M. Po p o v a , V.S. Ar b u z o v a , V56. Gi l s , M.: Development of an innovative hybrid seed pro- J. Sa l s e , C. Po n t , M.S. Rö d e r , A. Bö r n e r & L. I. La y k o v a (vor- duction system for wheat. – 10th GABI Status Seminar, getragen von Dobrovolskaya , O.B.): Molecular-genetic Potsdam, 09.-11.03.2010. and physical mapping of mutant genes involved in the V57. Gi l s , M.: Hybridzüchtung in Weizen. – GFP – Öffentli- inflorescence development in bread wheat and its close che Sitzung der Abteilung Getreide, IPK, Gatersleben, relatives. – International Conference: Plant Genetics, 10.06.2010. Genomics, and Biotechnology 2010 (PlantGen), Novo- V58. GILS, M.: Der Klimawandel als Herausforderung für sibirsk/Russia, 07.-10.06.2010. die Züchtung. – Besuch H. Brehmer, IPK, Gatersleben, V45. En t z i a n , A.: LemnaTec database architecture and im- 06.12.2010. age analysis. – Bayer CropScience, Frankfurt am Main, V59. Go e d e k e , S., G. He n s e l , C. Ka y d a m o v , J. Ma c e k , T. Ru t t e n , I. 07.12.2010. Sa a l b a c h & J. Ku m l e h n (vorgetragen von Go e d e k e , S.): Pro- V46. Fi n k e , A.: Genetic and molecular analysis of RNA-direct- duction of recombinant protein in transgenic barley ed transcriptional gene silencing (RdTGS) in Arabidopsis grains. – COST FAO804 Workshop “Molecular farming: thaliana. – 23.Tagung “Molekularbiologie der Pflanzen”, plants as a production platform for high value proteins”, Dabringhausen, 23.-26.02.2010. Wageningen/The Netherlands, 25.-26.01.2010. V47. Fl o r s c h ü t z , K., A. Sc h r ö t e r , M. Kö r n e r , S. Sc h m i e d e r , F. So n n - V60. Gr a n e r , A.: Bewahren und Verändern: Forschungsarbei- t a g , K. Ve t t e r , R. Wa t z k e & G. Ku n z e (vorgetragen von ten zur Nutzbarmachung pflanzlicher Biodiversität am Fl o r s c h ü t z , K.): Auf den Spuren von Pilzen und Viren IPK Gatersleben. – Museum für Naturkunde, Magde- mittels SPR und Plattenassay. – Technische Systeme für burg, 22.04.2010. die Lebenswissenschaften. – 15. Heiligenstädter Kollo- V61. Gr a n e r , A.: The Leibniz Institute of Plant Genetics and quium, Heiligenstadt, 27.-29.09.2010. Crop Plant Research (IPK). – 10th ECPGR Forages Work- V48. Fr a n k e , M., C. Kr a c h , H. Po s k a r & B. H. Ju n k e r (vorgetra- ing Group Meeting, Malchow (Poel), 28.-29.04.2010. gen von Fr a n k e , M.): Metabolic flux analysis in Medicago V62. Gr a n e r , A.: Saatgut und pflanzliche Leistung: Forschungs- truncatula. – 6th Plant Science Student Conference, IPK, ansätze zur züchterischen Verbesserung von Kultur- Gatersleben, 15.-18.06.2010. pflanzen. – Tagung „Megatrend Agrarrohstoffe”, K+S V49. Fr i e d e l , S.: Searching for pattens in nucleotide sequen- Kali GmbH, Kassel, 10.06.2010. ces. – Robert-Koch-Institut, Berlin, 20.10.2010. V63. Gr a n e r , A.: Von der Natur zur Kultur: Nutzpflanzen als V50. Fr i t s c h , R.M.: Taxonomy and molecular evolution of Ira- Lebensgrundlage des Menschen. Veranstaltungsreihe nian species of Allium subg. Melancrommyum. – Collo- „Melanchthons Erben”. – Kreisvolkshochschule Harz, quium of the Department of Botany, Iranian Research Quedlinburg, 17.06.2010. Institute of Plant Protection, Tehran/Iran, 12.05.2010. V64. Gr a n e r , A.: Grüne Gentechnik: Anmerkungen aus der V51. Fr i t s c h , R.M.: The genus Allium L. – how to classify an Wissenschaft. – Fraktionstagung DIE LINKE im Landtag extreme diversity. – Colloquium of the Department von Sachsen-Anhalt, Halle/S., 19.10.2010. of Plant Systematics, Botanical Institute of the Arme- V65. Gr a n e r , A.: Nutzung genetischer Vielfalt: Heraus- nian National Academy of Sciences, Jerewan/Armenia, forderungen und Perspektiven. – Fachtagung des 31.05.2010. Dachverband Agrarforschung (DAF), Braunschweig, V52. Fr i t s c h , R.M.: Iran – terra incognita Alliorum? – Colloqui- 20.-21.10.2010. um of the Department of Plant Systematics, Botanical V66. Gr a n e r , A.: Utilization of Plant Genetic Resources: a re- Institute of the Armenian National Academy of Scienc- search and technology perspective. – 1st International es, Jerewan/ Armenia, 31.05.2010. Expo Forum “Food Security and Agro-Biodiversity”, V53. Fu c h s , J.: Analyse pflanzlicher Genome mit Hilfe der Du- Milano/Italy, 18.-19.11.2010. rchflusszytometrie. – Gastkolloquium, Leibniz-Institut V67. Gr a n e r , A.: A peek into the future: Challenges of a se- für Gemüse- und Zierpflanzenbau, Großbeeren & Er- quence based valorization of plant genetic resources. furt, 18.11.2010. – 10th Gatersleben Research Conference 2010 (GRCX) V54. Ge r l a c h , T., J. Kn a u s t , C. Ka i s e r , M. Gi e r s b e r g , K. Sędzielewska , “Sequence-informed Crop Research”, Quedlinburg (Pa- M. Kö r n e r , K. Fl o r s c h ü t z , S. Uh l i g , K. Si m o n , K. Ku n a t h & G. lais Salfeldt)/Gatersleben (IPK), 22.-24.11.2010. Ku n z e (vorgetragen von Ge r l a c h , T.): Development of a V68. Gu s e , T., E. Gr ü t z e m a n n , J. Tu b i s , C. Ma r t h e & J. Ku m l e h n (vor- biosensor based on the yeast Arxula adeninivorans for getragen von Gu s e , T.): Transformation von Weizen du- the detection of androgenic activities in environmental rch Agrobakterien-vermittelten Gentransfer in in vitro samples. – 6th Plant Science Student Conference, IPK, kultivierten Samenanlagen – Gemeinsame Tagung Gatersleben, 15.-18.06.2010. des Arbeitskreises Deutsche In Vitro Kulturen (ADIVK) V55. Ge r s o n , L., K.J. De h m e r & S. Ha r t m a n n (vorgetragen von Ge r - und der Gesellschaft für Pflanzenbiotechnologie e.V. s o n , L.): Natural selection in perennial ryegrass (Lolium „Pflanzenbiotechnologie in Deutschland – wo stehen perenne L.) populations: developing selection methods wir?“, Hannover, 13.-15.09.2010. for the complex feature “persistence“. – 6th Plant Science V69. Ha i l e , J., K. Ha m m e r , M. Na c h i t , A. Ba d e b o & M. S. Rö d e r (vor- Student Conference, IPK, Gatersleben, 15.-18.06.2010. getragen von Ha i l e , J.): QTL analysis in an intraspecific recombinant inbred population of durum wheat (Triti-

170 cum turgidum L. var. durum) to recent Eastern African V83. Ja n k o w s k a , D., A. Tr a u t w e i n , K. Fa u l w a s s e r , A. Co r d e s & G. Ku n z e isolate of stem rust, Puccinia graminis f. sp. tritici, Ug99. – (vorgetragen von Ja n k o w s k a , D.): Arxula adeninivorans as a 6th Plant Science Student Conference, IPK, Gatersleben, protein producer towards the production of low-purine 15.-18.06.2010. content food. – 6th Plant Science Student Conference, V70. Ha j i r e z a e i , M.-R.: Improvement of biomass production: IPK, Gatersleben, 15.-18.06.2010. resources, opportunities and constraints. – 16th Inter- V84. Ja n k o w s k a , D., A. Tr a u t w e i n & G. Ku n z e (vorgetragen von national Conference on Renewable Resources and Ja n k o w s k a , D.): Arxula adeninivorans as an enzyme pro- Plant Biotechnology, NAROSSA®2010, Magdeburg, 07.- ducer towards the production of low-purine content 08.06.2010. food. – Institute of Microbial Technology (IMTECH); V71. Ha n e m a n n , A., N. We i c h e r t , H. Si m k o v a , J. Do l e z e l & M. S. Rö d e r Chandigarh/India, 15.12.2010. (vorgetragen von Rö d e r M. S.): Exploiting the genomic V85. Jo h n s t o n , A.J.: Retinoblastoma in cell differentiation. – sequences of rice and Brachypodium for delimiting a Universität Regensburg, 03.02.2010. grain size QTL in wheat. – 2nd International Symposium V86. Jo h n s t o n , A.J.: Epigenetic control of apomixis in Boechera on Genomics of Plant Genetic Resources, Bologna/Italy, species. – XXI International Congress on Sexual Plant 24.-27.04.2010. Reproduction, Bristol/UK, 02.-06.08.2010. V72. Ha u p t m a n n , V., B. Le p i c h i e r , F. Ju n g h a n s , M. Me n z e l , H. V87. Jo h n s t o n , A.J. & W. Gr u i s s e m (vorgetragen von Jo h n s t o n , A.J.): Ro g n i a u x , J. Gu e g u e n , U. Sp o h n & U. Co n r a d (vorgetragen The Retinoblastoma pathway: An evolutionary module von Ha u p t m a n n , V.): Assembly and plant-based expres- controlling plant life cycle. – XXI International Congress sion of spider silk-like proteins and their bio-mechani- on Sexual Plant Reproduction, Bristol/UK, 02.-06.08.2010. cal characterisation. – 6th Plant Science Student Confer- V88. Jo h n s t o n , A.J. & O. Kirioukhova (vorgetragen von Jo h n s t o n , ence, IPK, Gatersleben, 15.-18.06.2010. A. J.): RKD in plant evolution. – National Institute for Ba- V73. He d t m a n n , C., G. Ku n z e , G. d e Ja e g e r & H.-P. Mo c k (vorgetra- sic Biology, Okazaki/Japan, 01.12.2010. gen von He d t m a n n , C.): A co-chaperone works not alone, V89. Jo v t c h e v , G., J. Fu c h s & I. Sc h u b e r t (vorgetragen von Jo v t c h e v , but who is the counterpart? – 6th Plant Science Student G.): Distinct methylation marks characterize the sex chro- Conference, IPK, Gatersleben, 15.-18.06.2010. matin in Rumex acetosa. – Laboratory of Plant Develop- V74. He n s e l , G., D. No w a r a , G. Zi m m e r m a n n , A. Ga y , J. Ku m l e h n & mental Genetics Institute of Biophysics, Czech Academy P. Sc h w e i z e r (vorgetragen von He n s e l , G.): Resistenzer- of Sciences, Brno/Czech Republic, 26.-30.07.2010. höhung bei Gerste und Weizen mittels pflanzlicher V90. Ju n k e r , B.H.: GABI BASIS: SysSeed – Integrated modelling Expression von RNAi-Konstrukten mit konservierten of the primary metabolism of the developing barley Sequenzen pathogener Pilze. – Gemeinsame Tagung endosperm. – 10th GABI Status Seminar, Potsdam, 09.- des Arbeitskreises Deutsche In Vitro Kulturen (ADIVK) 11.03.2010. und der Gesellschaft für Pflanzenbiotechnologie e.V. V91. Ju n k e r , B.H.: Systems approaches to Medicago trunca- „Pflanzenbiotechnologie in Deutschland – wo stehen tula seed metabolism. – Joint 5th International Food Le- wir?“, Hannover, 13.-15.09.2010. gumes Research Conference & 7th European Conference V75. Ho a n g , P.T. & U. Co n r a d (vorgetragen von Ho a n g , P.T.): on Grain Legumes, Antalya/Turkey, 26.-30.04.2010. Expression of recombinant antigens of H5N1 avian flu V92. Ka p u s i , E., C.H. Te o , L. Ma, G. He n s e l , I. Sc h u b e r t , M.F. Me t t e virus in Nicotiana tabacum. – Riems, 23.03.2010. & J. Ku m l e h n (vorgetragen von Ka p u s i , E.): Engineered V76. Ho u b e n , A.: Biology of B chromosomes. – University of minichromosomes as vectors in barley. – Gemeinsame Zurich, Zurich/Switzerland, 23.02.2010. Tagung des Arbeitskreises Deutsche In Vitro Kulturen V77. Ho u b e n , A.: Biology of B chromosomes. – University of (ADIVK) und der Gesellschaft für Pflanzenbiotechnolo- Jammu/India, 27.04.2010. gie e.V. „Pflanzenbiotechnologie in Deutschland – wo V78. Ho u b e n , A.: Evolution of species via genome combina- stehen wir?“, Hannover, 13.-15.09.2010. tion. – University of Jammu/India, 28.04.2010. V93. Ka s p a r , S., A. Ma t r o s , O. Je n s e n , C. Fi n n i e , B. Sv e n s s o n & H.- V79. Ho u b e n , A.: Biology of B chromosomes. – University of P. Mo c k (vorgetragen von Ka s p a r , S.): Targeted protein Kashmir/India, 30.04.2010. analyses of barley grains to unveil spatial and tempo- V80. Ho u b e n , A.: Phosphorylation of histone H3 – a dy- ral distribution patterns. – Systems biology and Omic namic affair. – European Networking Summer School approaches (Joint meeting: FNRS protéomique & COST (ENSS) Plant Epigenetics 2010, IPK, Gatersleben, 20.- FA0603), Namur/Belgium, 05.-07.05.2010. 24.09.2010. V94. Ke i l w a g e n , J.: Jstacs: Diskriminative Motivsuche und V81. Ho u b e n , A.: Centromere-mediated instability in wide hy- mehr. – 17. IndustrieTag Informationstechnologie, brids. – Institutstag IPK, Gatersleben, 04.-05.10.2010. Halle/S., 16.11.2010. V82. Ja c o b , S.S.: “Almost a form of schizophrenia”: Wie Pop- V95. Ke l l e r , E.R.J. & A. Se n u l a (vorgetragen von Ke l l e r E. R. J.): ulationsdemographie Veränderungen der ökologi- Pollen cryopreservation as a tool for genebank mainte- schen Nische beeinflusst – am Beispiel diploider ameri- nance of germplasm. – COST Action 871, 4th Meeting of kanischer Gersten. – Senckenberg-Museum, Görlitz, WG 1, Fundamental Aspects of Plant Cryopreservation, 06.03.2010. Cryoprotection and Genetic Stability, Poznan/Poland, 29.-30.04.2010.

171 Vorträge und Poster/Lectures and Posters

V96. Ke l l e r , E. R. J.: Present situation of in vitro storage and V110. Ko h l , S.: Senescence-induced N remobilisation and seed cryopreservation in genebanks. InWent-Refresher-Kurs, filling in barley. – Arbeitstreffen DFG-Forschergruppe IPK Gatersleben, 18.05.2010. FOR 948 „Seneszenz“, Hohenlieth, 06.05.2010. V97. Ke l l e r , J.: Kryokonservierung und in vitro-Erhaltung als V111. Ko l l e r s , S., M.S. Rö d e r , V. Ko r z u n , E. Eb m e y e r , O. Ar g i l l i e r, P. Konservierungsstrategie für Genbanken – Gemeinsame Jo a q u i m , D. Ku l o s a , B. Ro d e m a n n & M. Ga n a l (vorgetragen Tagung des Arbeitskreises Deutsche In Vitro Kulturen von Ko l l e r, S.): Population structure in a collection of (ADIVK) und der Gesellschaft für Pflanzenbiotechnolo- elite West-European germplasm of hexaploid wheat gie e.V. „Pflanzenbiotechnologie in Deutschland – wo (Triticum aestivum L.). – Conference “Green Plant Breed- stehen wir?“, Hannover, 13.-15.09.2010. ing Technologies”, Vienna/Austria, 02.-05.02.2010. V98. Ke l l e r , E.R.J.: The EURALLIVEG project ‑ achievements V112. Kö s z e g i , D., A.J. Jo h n s t o n , A. Cz i h a l , U. Hä h n e l , J. Ku m l e h n , L. and problems. – Institutstag IPK, Gatersleben, 04.- Al t s c h m i e d , U. Grossniklaus & H. Bä u m l e i n (vorgetragen von 05.10.2010. Kö s z e g i , D.): RKD defines the egg cell identity in wheat V99. Kl u k a s , C.: Auswertung und Visualisierung von Hoch- and Arabidopsis. – International Conference “Molecular durchsatzdaten mittels VANTED. – Kolloquium der Aspects of Plant Development”, Vienna/Austria, 23.- Roux-Forschergruppe, Medizinische Fakultät der 26.02.2010. Martin-Luther-Universität Halle-Wittenberg, Halle/S., V113. Kr a c h , C., M. Fr a n k e , L. Fi c h t m ü l l e r, F. Ke l l n e r , H. Po s g a r , N. 28.01.2010. Sc h ä f e r & B. H. Ju n k e r (vorgetragen von Kr a c h , C.): Steady- V100. Kl u k a s , C.: Visualization and analysis of multimodal bio- state 13C metabolic flux analysis: Focus on developing logical data. – 2010 International Summer School on barley seeds. – XVII Conference of the Federation of Eu- Integrative Biological Pathway Analysis and Simulation, ropean Societies of Plant Biology (FESPB 2010), Valen- Universität Bielefeld, 21.05.2010. cia/Spain, 04.-09.07.2010. V101. Kl u k a s , C.: Visualization of biological experiment data in V114. Ku m l e h n , J., L. Al t s c h m i e d , F. Ba k o s , H. Bä u m l e i n , D. Da g h m a , context of networks and pathway drawings according C. Gr y c z k a , G. He n s e l , D. Kö s z e g i , R. Li p p m a n n , A. Ma t r o s , M. to the SBGN standard, outlook to automated phenotyp- Me l z e r , H.-P. Mo c k , K. Pl a s u n , T. Ru t t e n & C. Sp r i n g m a n n (vor- ing at the IPK. – College of Life Sciences, Zhejiang Uni- getragen von Ku m l e h n , J.): Initial mechanisms of pollen versity, Hangzhou/China, 29.07.2010. embryogenesis. – 10th GABI Status Seminar, Potsdam, V102. Kl u k a s , C.: Automated phenotyping with the Integrated 09.-11.03.2010. Analysis Platform (IAP). – Bayer CropScience, Frankfurt V115. Ku h l m a n n , M., R. Sc h m i d t & M. F. Me t t e (vorgetragen von am Main, 07.12.2010. Ku h l m a n n , M.): Histone modifications in RNA-directed V103. Kl u k a s , C.: Development of the Integrated Analysis transcriptional gene silencing. – XVII Conference of Platform (IAP). – BASF Plant Science, Limburgerhof, the Federation of European Societies of Plant Biology 18.10.2010. (FESPB 2010), Valencia/Spain, 04.-09.07.2010. V104. Kn ü p f f e r , H.: Report from the conference TDWG-2009, V116. Ku h l m a n n , M., R. Sc h m i d t & M. F. Me t t e (vorgetragen von Montpellier, November 2009. – ECPGR Documentation Ku h l m a n n , M.): Genetic analysis of RNA-mediated gene and Information Network Coordinating Group (NCG), silencing in Arabidopsis thaliana. – 6th Genetic Work- Maccarese/ Italy, 17.-18.02.2010. shop on small non-coding RNAs, Berlin, 24.-25.11.2010. V105. Kn ü p f f e r , H.: Genbankdokumentation und Informations- V117. Ku n z e , G.: Arxula adeninivorans. – Institut Pasteur, netze für pflanzengenetische Ressourcen undB iodiver- Genomes and Genetics Department, Paris/France, sität. Bioinformatik-Intensivkurs für Studenten der Uni- 10.05.2010. versität Kiel, IPK Gatersleben, 19.03.2010. V118. Ku n z e , G.: Yeast – a valuable tool for expression of bio- V106. Kn ü p f f e r , H. & T. J. L. v a n Hi n t u m (vorgetragen von Kn ü p f f e r , sensor compounds and for biofuel cells. – Christchurch/ H.): Comparison of the coverage of European Central New Zealand, 23.11.2010. Crop Databases and EURISCO with respect to forage V119. La n g e , M.: Das Modell des Information Retrieval und crop species. – 10th ECPGR Forages Working Group seine Anwendung in der Praxis. – Vortrag im Berufungs- Meeting, Malchow (Poel), 28.-29.04.2010. verfahren Informationsmanagement, Hochschule An- V107. Kn ü p f f e r , H.: Genebank documentation and information halt (FH), Köthen, 08.01.2010. networks for plant genetic resources and biodiversity. V120. La n g e , M.: The LAILAPS search engine: a feature model InWent Refresher Course “The Role of Plant Genetic for relevance ranking in life science databases. – 6th In- Resources in Climate Change Adaptation” Gatersleben, ternational Symposium on Integrative Bioinformatics 18.05.2010. 2010, Cambridge/UK, 22.-24.03.2010. V108. Kn ü p f f e r , H.: Genbankdokumentation und Informations- V121. La n g e , M.: Databases and information systems in Bioin- netze für pflanzengenetische Ressourcen undB iodiver- formatics: from information retrieval to search engines. sität. IPK Gatersleben, Vortrag vor Studenten der Uni- – International Summer School on Integrative Biological versität Göttingen, 16.06.2010. Pathway Analysis and Simulation, Universität Bielefeld, V109. Kn ü p f f e r , H.: Analysis of historical observation data of 20.05.2010. IPK genebank accessions of barley. – Institutstag IPK, V122. Le o n o v a , I.N., E.B. Bu d a s h k i n a , N.P. Ka l i n i n a , M.S. Rö d e r , E.S. Gatersleben, 04.-05.10.2010. Sk o l o t n e v a , A. Bö r n e r & E. A. Sa l i n a (vorgetragen von Le-

172 o n o v a , I. N.): T. aestivum × T. timopheevii introgression wide and candidate gene approach. – Conference lines as a source of pathogen resistance genes. – 8th “Green Plant Breeding Technologies”, Vienna/Austria, International Wheat Conference, St. Petersburg/Russia, 02.-05.02.2010. 01.-04.06.2010. V135. Ma t t h i e s , I., M.S. Rö d e r , R.K. Pa s a m , B. Kilian & A. Gr a n e r V123. Li p p m a n n , R., A. Ma t r o s , J. Ku m l e h n & H.-P. Mo c k (vorgetra- (vorgetragen von Rö d e r , M.S.): Assoziationsgenetik bei gen von Li p p m a n n , R.): Identification of potential feeder Gerste. – GFP Sommertagung Getreide, IPK, Gatersle- substances promote pollen embryogenesis (POEM) ben, 09.-10.06.2010. from the secretome of pistils. – 6th Plant Science Stu- V136. Ma u , M. & T. F. Sh a r b e l (vorgetragen von Ma u , M.): Unre- dent Conference, IPK, Gatersleben, 15.-18.06.2010. duced pollen formation in Boechera – a transcriptomic V124. Lo h w a s s e r , U., M. Na g e l & A. Bö r n e r (vorgetragen von study. – 6th Plant Science Student Conference, IPK, Lo h w a s s e r , U.): Untersuchungen zur Langlebigkeit und Gatersleben, 15.-18.06.2010. Langzeitlagerung von Saatgut. – 20. Bernburger Win- V137. Me h l h o r n , H.: The DBE2 information system. – SEA Pro- terseminar für Arznei- und Gewürzpflanzen, Bernburg, ject Meeting, IPK, Gatersleben, 21.-22.01.2010. 23.02.2010. V138. Me h l h o r n , H.: The DBE2 information system. – Task Force V125. Lo h w a s s e r , U. & A. Bö r n e r (vorgetragen von Lo h w a s s e r , U.): Metabolite Profiling, IPK, Gatersleben, 26.02.2010. Saatgutqualität im Wandel der Zeit – Von der Domes- V139. Me h l h o r n , H.: Ontologies – arrangements of beings. – tikation zum Qualitätsmanagement. – Arbeitstagung 6th Plant Science Student Conference, IPK, Gatersleben, der Arbeitsgemeinschaft Saatgut- und Sortenwesen 15.-18.06.2010. der GPZ und GPW, IPK, Gatersleben, 24.-25.02.2010. V140. Me l z e r , M.: Recent developments in modern micro- V126. Lo h w a s s e r , U.: Saatgutqualität im Wandel der Zeit – Von scopy: Methods and application. – Bhabha Atomic Re- der Domestikation zum Qualitätsmanagement. – Saat- search Centre, Mumbai/India, 19.03.2010. bautagung 2010 des Saatbauverbandes Sachsen-An- V141. Me l z e r , M.: Recent developments in modern microsco- halt, Bernburg-Strenzfeld, 10.03.2010. py: Methods and application. – TATA Institute of Funda- V127. Lo h w a s s e r , U. & A. Bö r n e r (vorgetragen von Lo h w a s s e r , U.): mental Research (TIFR), Mumbai/India, 24.03.2010. Die bundesdeutsche ex situ-Genbank in Gatersleben, V142. Me t t e , M.F.: RNA-directed DNA methylation in plants. – Erhalt pflanzengenetischer Ressourcen. – Weihensteph- European Networking Summer School (ENSS) Plant Epi- aner Symposium zur Gartendenkmalpflege, Freising- genetics 2010, IPK, Gatersleben, 20.-24.09.2010. Weihenstephan, 25.06.2010. V143. Me t t e , M.F.: RNA-directed RNA polymerases involved in V128. Lo h w a s s e r , U., T. Br u c h m ü l l e r , H. Bu d a h n , H. Kr ü g e r , D. Ul r i c h , A. transcript level-mediated post-transcriptional gene si- Bö r n e r & F. Ma r t h e (vorgetragen von Lo h w a s s e r , U.): Ergeb- lencing in Arabidopsis thaliana. – Institutstag IPK, Gater- nisse eines Genpoolscreenings bei Petersilie (Petroselinum sleben, 04.-05.10.2010. crispum (Mill.) Nyman) hinsichtlich wirtschaftlich be- V144. Me y e r , R.C., B. Eb e r t , T. Gä r t n e r , B. Ku s t e r e r , J. Li s e c , D. Ri e w e , deutsamer Merkmale. – Tagung der GPZ-AG Gemüse und R. Sc h m i d t , M. St e i n f a t h , K. We i g e l t , A.E. Me l c h i n g e r , J. Se l - Arznei- und Gewürzpflanzen und der GFP Abteilung Ge- b i g , L. Wi l l m i t z e r & T. Al t m a n n (vorgetragen von Al t m a n n , müse, Heil- und Gewürzpflanzen, Erfurt, 07.-08.09.2010. T.): Molecular and genetic analysis of biomass and me- V129. Lo h w a s s e r , U.: “Leafy Vegetables“ – ein europäisches Pro- tabolite heterosis in Arabidopsis thaliana. – Genomics- jekt zur verbesserten Nutzbarmachung verschiedener based breeding, Justus-Liebig-Universität, Gießen, 26.- Blattgemüsearten. – GFP-Jahrestagung, Abteilung Ge- 28.10.2010. müse, Heil- und Gewürzpflanzen, Bonn, 02.-04.11.2010. V145. Mi e d a n e r , T., M. Lo e f f l e r , P. Ri s s e r , L. Ha r t l , G. Strittmatter , V130. Lo h w a s s e r , U.: Leafy vegetables, stimulating use – char- M. Ou z u n o v a , B. Ho l t s c h u l t e , B. Ke s s e l , V. Ko r z u n , E. Eb m e y e r , acterisation and regeneration. – Leafy Vegetable Meet- P. Ka r l o v s k y , I. Fe u s s n e r , S. Nu t z , P. Sc h w e i z e r , J. Ku m l e h n , D. ing, Avignon/France, 16.-17.11.2010. Mu e l l e r , D.J. St a h l , F. Fe l s e n s t e i n , S. Pr a u d , M. Tr o t t e t , S.G. V131. Ma t r o s , A., S. Ka s p a r , U. Se i f f e r t , W. We s c h k e & H.-P. Mo c k At i e n z a Pe n a s , M.J. Gi m é n e z & B. Ke l l e r (vorgetragen von (vorgetragen von Ma t r o s , A.): Elucidation of tempo- Mi e d a n e r , T.): Securing a sustainable production of feed ral and spatial protein patterns in developing barley and food – a functional genomic-guided strategy for grains by multiplexed LC-MS and MALDI-imaging MS improving biotic stress tolerance in cereals. – 10th GABI approaches. – 43. Jahrestagung der Deutschen Gesell- Status Seminar, Potsdam, 09.-11.03.2010. schaft für Massenspektrometrie (DGMS), Halle/S., 07.- V146. Mo c k , H.-P.: Proteomics to evaluate genetic resources for 10.03.2010. stress defence responses. – Charles University, Prague/ V132. Ma t r o s , A.: LC-based protein analysis of barley grain Czech Republic, 13.04.2010. development. – German Proteomics Summit, Freising- V147. Mo c k , H.-P.: Proteomics to evaluate genetic resources for Weihenstephan, 06.05.2010. stress defence responses. – University of Silesia, Kato- V133. Ma t r o s , A.: Proteomic approaches for analysis of bar- wice/Poland, 23.06.2010. ley grain development. – Massenspektrometrie Forum V148. Mo c k , H.-P.: Proteomics to evaluate genetic resources Hannover, Hannover, 15.06.2010. for stress defence responses. – Frontier in Agriculture V134. Ma t t h i e s , I., S. We i s e & M.S. Rö d e r (vorgetragen von Ma t - Proteome Research, National Institute of Crop Science, t h i e s , I.): Association mapping in barley – a genome Tsukuba/Japan, 18.11.2010.

173 Vorträge und Poster/Lectures and Posters

V149. Mu t h , J., T. Al t m a n n , E. Ba u e r , S. Fr e r i c h m a n n , H.-J. Ha r l o f f , V161. Op p e r m a n n , M.: General aspects of Genebank Informati- C. Ju n g , V. Ko r z u n , T. Kr a f t , J. Ku m l e h n , G. Le c k b a n d , M. Ou- on Systems, – InWent-Refresher-Kurs, IPK Gatersleben, z u n o v a , D. Pr ü f e r , N. St e i n , D. St e l l i n g , E. Ta c k e & B. We i s s h a a r 18.05.2010. (vorgetragen von Mu t h , J.): GABI-TILL: Expansion and use V162. Ou z u n o v a , M., T. Pr e s t e r l , P. We s t h o f f , K. Er n s t , E. Pe s t s o v a , of the GABI-TILLING platform for crop gene function anal- T. Al t m a n n , R.C. Me y e r , M. Er n s t , A.E. Me l c h i n g e r , J.M. Mo n - ysis. – 10th GABI Status Seminar, Potsdam, 09.-11.03.2010. t e s , J. Se l b i g , M. St i t t , R. Su l p i c e , A. Cz e d i k -Ey s e n b e r g & L. V150. Na g e l , M., I. Kr a n n e r & A. Bö r n e r (vorgetragen von Na g e l , Wi l l m i t z e r (vorgetragen von Al t m a n n , T.): Biomass pro- M.): Langlebigkeit von Getreidesamen – Physiologie duction in maize – genomics guided breeding of en- und Genetik. – Arbeitstagung der Arbeitsgemeinschaft ergy maize and a systems-oriented analysis. – 10th GABI Saatgut- und Sortenwesen der GPZ und GPW, IPK, Status Seminar, Potsdam, 09.-11.03.2010. Gatersleben, 24.-25.02.2010. V163. Pa l i w a l , R., A.K. Jo s h i , U. Ku m a r & M. S. Rö d e r (vorgetragen V151. Na g e l , M., I. Kr a n n e r & A. Bö r n e r (vorgetragen von Na- von Pa l i w a l , R.): M-QTL and epistasis contribute to ter- g e l , M.): Parameters related to seed longevity in cereal minal heat tolerance in hexaploid wheat (T. aestivum L.). seeds. – Departmental Science and Conservation Semi- – 6th Plant Science Student Conference, IPK, Gatersle- nar Series, Millennium Seed Bank Project, Wakehurst ben, 15.-18.06.2010. Place, West Sussex/UK, 17.03.2010. V164. Pe l l i n o , M. & T. F. Sh a r b e l (vorgetragen von Pe l l i n o , M.): V152. Na g e l , M.: The longevity of crop seeds stored under Effects of hybridization on expression of apomixis in ambient conditions. – Research Meeting, Millennium Ranunculus auricomus. – EU COST Apomixis Workshop, Seedbank, KEW Botanical Gardens/Wakehurst Place, Wageningen/The Netherlands, 05.07.2010. West Sussex/UK, 09.05.2010. V165. Pl a s u n , K., M. Mü l l e r, M. Me l z e r , B. Me l z e r , T. Al t m a n n & J. V153. Na g e l , M.: The genetic architecture of maize flowering Ku m l e h n (vorgetragen von Pl a s u n , K.): Haploid technol- time. – New Areas in Plant Breeding, PAG (Promotions- ogy in the major experimental model plants Arabidop- studiengang Agrar): Modul – Vertiefung des Fachwis- sis thaliana and Brachypodium distachyon: An initial sens, Georg-August-Universität, Göttingen, 09.07.2010. approach. – 6th Plant Science Student Conference, IPK, V154. Na g e l , M., I. Kr a n n e r & A. Bö r n e r (vorgetragen von Na g e l , Gatersleben, 15.-18.06.2010. M.): Seed conservation in ex situ gene banks; geneti- V166. Po s g a r , H. & B. H. Ju n k e r (vorgetragen von Po s g a r , H.): cal and physiological backgrounds for viability loss in Metabolic flux analysis. – Dechema Summer School wheat and barley after storage. – 29th Congress of the “Quantitative Biology – From Cell to Process”, Bad Her- International Seed Testing Association (ISTA), Köln 2010, renalb, 26.-30.07.2010. 15.-18.06.2010. V167. Poursarebani , N., R. Ar i y a d a s a , D. Sc h u l t e , R. Zh o u , B. St e u e r - V155. Ne u m a n n , K.: Assoziationskartierung von Trockentoleranz n a g e l , M.M. Ma r t i s , K. Ma y e r , A. Gr a n e r & N. St e i n (vor- in Gerste. – Oberseminar Pflanzenzüchtung, Martin-Lu- getragen von Poursarebani , N.): Genetic anchoring of ther-Universität Halle-Wittenberg, Halle/S., 08.07.2010. the physical map of barley (Hordeum vulgare L.) using V156. Nü r k , N.M.: Tracing the impact of the Andean uplift – a virtual gene map order of the barley genome. – 6th Evolutionary history of Hypericum in South America: Plant Science Student Conference, IPK, Gatersleben, biogeography, character evolution, niche shifts and 15.-18.06.2010. age estimations. – DFG Nachwuchsakademie „Systema- V168. Pu e n t e Mo l i n s , M., J.M. Co r r a l , O.M. Al i y u , J.L. Ma r o n & T. F. tik der Pflanzen und Pilze”, Senckenberg-Naturmuseum, Sh a r b e l (vorgetragen von Pu e n t e Mo l i n s , M.): Hybridiza- Frankfurt am Main, 25.09.2010. tion and its role in the reproduction of Hypericum per- V157. Nü r k , N.M., S. Ma d r i ñ á n , M. Ca r i n e , M. Ch a s e & F. R. Bl a t t - foratum. – Botany 2010, Providence, Rhode Island/USA, n e r (vorgetragen von Nü r k , N. M.): Molecular phylogeny 02.-06.08.2010. and historical biogeography of Hypericum. – 3rd Annual V169. Re h m a n Ar i f , M.A., U. Lo h w a s s e r & A. Bö r n e r (vorgetragen Hypericum Meeting, University of Padua, Padua/Italy, von Re h m a n Ar i f , M. A.): Seed longevity in wheat (Triticum 29.10.2010. aestivum L.) – Genetics and beyond. – Arbeitstagung V158. Nü r k , N.M., S. Ur i b e -Co n v e r s , D. Ta n k & F. R. Bl a t t n e r (vor- der Arbeitsgemeinschaft Saatgut- und Sortenwesen getragen von Nü r k , N. M.): Out of the tropics? Parametric der GPZ und GPW, IPK, Gatersleben, 24.-25.02.2010. model based biogeography reconstructions in the tem- V170. Re h m a n Ar i f , M.A., M. Na g e l , U. Lo h w a s s e r & A. Bö r n e r (vorge- perate genus of St. John’s wort (Hypericum). – PuRGe tragen von Re h m a n Ar i f , M. A.): Seed longevity in wheat (Trit- Seminar, University of Idaho & University of Washing- icum aestivum L.): genetics and beyond. – 6th Plant Science ton, Moscow/USA, 19.11.2010. Student Conference, IPK, Gatersleben, 15.-18.06.2010. V159. Op p e r m a n n , M.: Das Genbankinformationssystem GBIS. V171. Re h m a n Ar i f , M.A.: Genetics of seed longevity in wheat (Triti- Ein Überblick. – Arbeitstagung der Arbeitsgemein- cum aestivum L.). – Oberseminar Pflanzenzüchtung, Martin- schaft Saatgut- und Sortenwesen der GPZ und GPW, Luther-Universität Halle-Wittenberg, Halle/S., 04.11.2010. IPK, Gatersleben, 24.-25.02.2010. V172. Rö d d e r , D., S.S. Ja k o b , C. He i b l & F. R. Bl a t t n e r (vorgetragen V160. Op p e r m a n n , M.: Suggestions: handling of C&E data in the von Rö d d e r , D.): Linking large-scale analyses of climate European context. – 10th ECPGR Forages Working Group niche evolution with population demography. – GfÖ Meeting, Malchow (Poel), 27.-29.04.2010. 40th Anniversary Conference, Gießen, 30.08.-03.09.2010

174 V173. Rö d e r , M.S.: New genes by forward genetics approaches. V188. Sc h m i d t , R.: Analysis of allelic diversity in Brassica napus. – Institutstag IPK, Gatersleben, 04.-05.10.2010. – 10th Gatersleben Research Conference 2010 (GRCX) V174. Ru t t e n , T.: Role of transcription factor LEC1 (Leafy Coty- “Sequence-informed Crop Research”, Quedlinburg (Pa- ledon 1) in Arabidopsis embryogenesis. – Julius Kühn- lais Salfeldt)/Gatersleben (IPK), 22.-24.11.2010. Institut, Braunschweig, 23.03.2010. V189. Sc h m u t z e r , T., B. St e u e r n a g e l , F. Bu l l , U. Sc h o l z , A. Ho u b e n & V175. Ru t t e n , T., M. Me l z e r & D. Da g h m a (vorgetragen von Ru t - N. St e i n (vorgetragen von Sc h m u t z e r , T.): K-mer analysis to t e n , T.): High Pressure Freezing of immature pollen from reveal genomic secrets in highly repetitive genomes. – barley using cyanobacteria as “Filler“. – Frühjahrstreffen 6th Plant Science Student Conference, IPK, Gatersleben, des Arbeitskreises PANOS der Deutschen Gesellschaft 15.-18.06.2010. für Elektronenmikroskopie, Martin-Luther-Universität V190. Sc h n u r b u s c h , T.: Chasing genes for abiotic stress toler- Halle-Wittenberg, Halle/S., 07.05.2010. ance in wheat and barley. – Kolloquium in Pflanzen- V176. Sa n e i , M., R. Pickering, S. Na s u d a & A. Ho u b e n (vorgetragen züchtung, Martin-Luther-Universität Halle-Wittenberg, von Sa n e i , M.): Analysis of uniparental elimination of Halle/S., 22.01.2010. chromosome in wide crosses. – 6th Plant Science Stu- V191. Sc h n u r b u s c h , T.: Abiotischer Stress bei Weizen, die aus- dent Conference, IPK, Gatersleben, 15.-18.06.2010. tralische Sicht. – DLG-/GPZ-/GFP-Symposium: Ertrags- V177. Sc h a l l a u , A., F. Ar z e n t o n , A.J. Jo h n s t o n , U. Hä h n e l , D. Ko- bildung bei Weizen, Gustav-Stresemann-Institut, Bonn, s z e g i , F.R. Bl a t t n e r , L. Al t s c h m i e d , G. Ha b e r e r , G. Ba r c a c c i a & 01.-02.03.2010. H. Bä u m l e i n (vorgetragen von Bä u m l e i n , H.): Identification V192. Sc h n u r b u s c h , T.: Chasing genes for boron toxicity tol- and genetic analysis of the APOSPORY locus in Hyperi- erance in small grain cereals wheat and barley. – Kol- cum perforatum. – XXI International Congress on Sexual loquium, Technische Universität München-Freising, Plant Reproduction, Bristol/UK, 02.-06.08.2010. 24.06.2010. V178. Sc h a l l a u , K.: From subcellular data to kinetic models. – V193. Sc h n u r b u s c h , T.: The boron toxicity tolerance locus Bo1 Institut für Theoretische Biologie, Humboldt-Universität in bread wheat (Triticum aestivum L.) encodes a boron zu Berlin, 25.02.2010. efflux-type transporter specific to the genus Aegilops. V179. Sc h a l l a u , K.: Kinetic characteristices of AGPase in barley. – International Triticeae Mapping Initiative (ITMI) Sum- – Conference on Regulation of Plant Growth, Potsdam, mer Workshop, Beijing/China, 01.-04.09.2010. 12.-14.04.2010. V194. Sc h n u r b u s c h , T.: Are branched spikes advantageous? V180. Sc h l i c h t i n g , A.: Evaluierung genetischer Ressourcen der – The molecular elucidation of spike branching in Kartoffel durch innovative Analysen von Inhaltsstoff- wheat and barley. – Institutstag IPK, Gatersleben, 04.- und Qualitätsparametern der Knolle. – BLE-Innovations- 05.10.2010. tage, Berlin, 06.-07.10.2010. V195. Sc h o l z , U.: Assembling the barley genome possibilities, V181. Sc h m i d , N., A.R. Me d a & N. v o n Wi r é n (vorgetragen von Sc h m i d , challenges and current status. – Institutstag IPK, Gaters- N.): A role of the SNARE protein VTI11 in intracellular iron leben, 04.-05.10.2010. efficiency in Arabidopsis thaliana. – 6th Plant Science Stu- V196. Sc h r e i b e r , F.: Integrative Bioinformatics – Examples from dent Conference, IPK, Gatersleben, 15.-18.06.2010. high-throughput phenotyping and systems biology V182. Sc h m i d t , B.: The Cucurbitaceae of the German ex situ gen- graphical notation. – ACPFG Adelaide and University of ebank. – ECPGR Cucurbits Working Group, 2nd Meeting, South Australia, Adelaide/Australia, 21.01.2010. Tiblisi/Georgia, 08.-10.11.2010. V197. Sc h r e i b e r , F.: Integrative Bioinformatics. – DSTO, Mel- V183. Sc h m i d t , R.: Analysis of seed oil biosynthesis using natu- bourne/Australia, 28.01.2010. ral variation in Arabidopsis thaliana and Brassica napus. – V198. Sc h r e i b e r , F.: Integrative Bioinformatics – Examples from Biowissenschaftliches Netzwerk„Strukturen und Mecha- modelling plant metabolism and systems biology nismen der biologischen Informationsverarbeitung“, graphical notation. – Monash University, Melbourne/ Gesamtarbeitsbesprechung, Martin-Luther-Universität Australia, 03.02.2010. Halle-Wittenberg, Halle/S., 04.-05.03.2010. V199. Sc h r e i b e r , F.: Integrative Bioinformatics – network model- V184. Sc h m i d t , R.: Genome plasticity in Brassicaceae. – Institut ling, analysis, and visualisation with examples from crop für Pflanzenbau und Pflanzenzüchtung, Christian-Al- plant metabolism. – The University of Western Australia, brechts-Universität zu Kiel, 04.05.2010. Perth/Australia, 10.02.2010. V185. Sc h m i d t , R.: Analysis of allelic diversity in Brassica napus. V200. Sc h r e i b e r , F.: Systems Biology Graphical Notation (SBGN) – Brassica2010, 17th Crucifer Genetics Workshop, Saska- – methods and tools. – 2010 International Summer toon/Canada, 05.-09.09.2010. School on Integrative Biological Pathway Analysis and V186. Sc h m i d t , R.: Transcript level-mediated post-transcrip- Simulation, Universität Bielefeld, 21.05.2010. tional gene silencing. – European Networking Summer V201. Sc h r e i b e r , F.: Biological networks. – Workshop proGD, School (ENSS) Plant Epigenetics 2010, IPK, Gatersleben, Konstanz, 21.09.2010. 20.-24.09.2010. V202. Sc h r e i b e r , F.: Modeling, analysis, simulation and visuali- V187. Sc h m i d t , R.: Analysis of allelic diversity in Brassica napus. sation of biological networks. – Maastricht University, – Institutstag IPK, Gatersleben, 04.-05.10.2010. Maastricht/The Netherlands, 05.11.2010.

175 Vorträge und Poster/Lectures and Posters

V203. Sc h r e i b e r , F.: Systems Biology Graphical Notation – eine Animal Genome XVIII. Conference, San Diego/USA, 09.- neue Sprache der Biologen mit Unterstützung aus der 13.01.2010. Informatik. – Dagstuhl Workshops Wissenschaftsjourna- V216. Sc h w e i z e r , P.: Gene für quantitative Krankheitsresistenz lismus „Schreiben und Sprechen über Informatik“, Dag- in der Gerste. – GFP – Öffentliche Sitzung der Abtei- stuhl Leibniz-Zentrum für Informatik, 14.-16.06.2010. lung Getreide, IPK, Gatersleben, 10.06.2010. V204. Sc h r e i b e r , F.: Integrative bioinformatics – network mod- V217. Sc h w e i z e r , P.: RNA interference as a tool in crop plant ge- elling, analysis, and visualisation with examples from netic analysis. – European Networking Summer School crop plant metabolism. – CSIRO Canberra/Australia, (ENSS) Plant Epigenetics 2010, IPK, Gatersleben, 20.- 26.11.2010. 24.09.2010. V205. Sc h r e i b e r , F.: Systems biology graphical notation. – V218. Sc h w e i z e r , P.: A role of the GER4 gene cluster in quantita- The University of Western Australia, Perth/Australia, tive resistance of barley. – Institutstag IPK, Gatersleben, 07.12.2010. 04.-05.10.2010. V206. Sc h r e i b e r , F.: An introduction to flux balance analysis and V219. Sc h w e i z e r , P.: Ein biotechnologischer Ansatz zur Reduk- Vanted/FBASimVis. – The University of Western Austra- tion des Fusariumbefalls in Getreide. – 3. Wiss. Sym- lia, Perth/Australia, 08.12.2010. posium des Verbandes Deutscher Mühlen, Würzburg, V207. Sc h r e i b e r , F.: Analysis and visualisation of biological net- 05.11.2010. works and related information – barley as an example. V220. Sędzielewska , K., K. Ve t t e r , J. Fu c h s , N.M. Nü r k , R. Bo d e , R. – Workshop on Analysis and Visualisation of Large and Wa t z k e & G. Ku n z e (vorgetragen von Sędzielewska , K.): Complex Data Sets, Sydney/Australia, 13.12.2010. Symbiotic glomeromycota Glomus intraradices – diag- V208. Sc h u b e r t , I.: Mechanics of chromosome and karyotype nostic investigation. – 6th Plant Science Student Confer- evolution – a magnifying lens for palaeogenomics. – ence, IPK, Gatersleben, 15.-18.06.2010. Annual Conference of the German Genetics Society V221. Se i f e r t , M.: Exploiting prior knowlegde and gene dis- (GfG) “Evolution of Primates”, Jena, 16.-18.09.2010 tances in the analysis of tumor expression profiles with V209. Sc h u b e r t , I.: Modifications of plant interphase chromatin extended Hidden Markov Models. – Universität Siegen, at the microscopic level – an overview. – European Net- 19.12.2010. working Summer School (ENSS) Plant Epigenetics 2010, V222. Se y f a r t h , M.: Identification and characterization of func- IPK, Gatersleben, 20.-24.09.2010. tional diversity of regulatory proteins controlling plant V210. Sc h u b e r t , I.: Interphase chromosome arrangement is growth. – Biowissenschaftliches Netzwerk „Strukturen coined by genetic and environmental impacts and de- und Mechanismen der biologischen Informationsver- termines genome stability. – Institutstag IPK, Gatersle- arbeitung“, Gesamtarbeitsbesprechung, Martin-Luther- ben, 04.-05.10.2010. Universität Halle-Wittenberg, Halle/S., 04.-05.03.2010. V211. Sc h u b e r t , I.: Mechanics of chromosome evolution and V223. Sh a r b e l , T.F., M.-L. Vo i g t , J.M. Co r r a l , G. Ga l l a , J. Ku m l e h n , C. potential pitfalls of genomics. – Mini-Symposium Kl u k a s , F. Sc h r e i b e r , H. Vo g e l & B. Ro t t e r (vorgetragen von “Plants and the evolution their genomes” on the occa- Sh a r b e l , T. F.): Apomictic and sexual ovules of Boechera sion of the retirement of Prof. Johann Greilhuber, Uni- display heterochronic global gene expression patterns. versity of Vienna, Vienna/Austria, 05.11.2010. – Plant and Animal Genome XVIII. Conference, San Di- V212. Sc h u b e r t , I. & M. Ly s a k (vorgetragen von Sc h u b e r t , I.): Me- ego/USA, 09.-13.01.2010. chanics of chromosome evolution – a magnifying lens V224. Sh a r b e l , T.F.: Sex and agriculture. – Universität Bayreuth, for paleogenomics. – 10th Gatersleben Research Confer- 28.01.2010. ence 2010 (GRCX) “Sequence-informed Crop Research”, V225. Sh a r b e l , T.F.: Sex and agriculture. – Systems Biology Quedlinburg (Palais Salfeldt)/Gatersleben (IPK), 22.- Workshop, Victoria AgriBiosciences Center, Melbourne/ 24.11.2010. Australia, 17.-28.05.2010. V213. Sc h u l t e , D., R. Ar i y a d a s a , N. Poursarebani , R. Zh o u , T. Sr e t e - V226. Sh a r b e l , T.F.: Heterochrony and hybridization: the evolution n o v i c -Rajicic, P. La n g r i d g e , B.-J. Sh i , K. Ma y e r , T. Cl o s e , S. We i s e , of apomixis in the genus Boechera. – Brassica2010,17th U. Sc h o l z , A. Gr a n e r & N. St e i n (vorgetragen von Sc h u l t e , Crucifer Genetics Workshop, Saskatoon/Canada, 05.- D.): Whole genome physical map of barley (Hordeum 09.09.2010. vulgare L.). – Plant and Animal Genome XVIII. Confer- V227. Sh a r b e l , T.F.: Time travel: a conserved apomixis-specific ence, San Diego/USA, 09.-13.01.2010. functional polymorphism has recombined and spread V214. Sc h u l t e , D., R. Ar i y a d a s a , T. Sc h m u t z e r , P. La n g r i d g e , B.-J. during the post-Pleistocene evolution of apomictic Sh i , A. Gr a n e r & N. St e i n (vorgetragen von Sc h u l t e , D.): Boechera. – Institutstag IPK, Gatersleben, 04.-05.10.2010. Contribution of a random sheared library to whole ge- V228. Sh a r b e l , T.F.: Sex and agriculture: evolutionary genom- nome physical mapping in barley (Hordeum vulgare cv. ics and next generation sequencing approaches to Morex). – Plant and Animal Genome XVIII. Conference, understanding apomixis. – II Ciclo de seminarios sobre San Diego/USA, 09.-13.01.2010. avances en la caracterización genética y molecular de la V215. Sc h w e i z e r , P.: Multiple WRKY-factor binding sites in the apomixis en gramíneas forrajeras, Facultad de Ciencias promoters of the GER4 gene cluster of barley cause Agrarias, Universidad Nacional de Rosario, Argentina, high transcription upon pathogen attack. – Plant and 23.-24.11.2010.

176 V229. Sh a r b e l , T.F., M.-L. Vo i g t , J.M. Co r r a l , J. Ku m l e h n , C. Kl u k a s , V243. St e i n , N.: How do we access genes in Triticeae genetic F. Sc h r e i b e r , H. Vo g e l & B. Ro t t e r (vorgetragen von Sh a r - centromeres? – 10th Gatersleben Research Conference b e l , T. F.): Heterochrony and hybridization: the evolution 2010 (GRCX) “Sequence-informed Crop Research”, of apomixis in the genus Boechera. – 10th Gatersleben Quedlinburg (Palais Salfeldt)/Gatersleben (IPK), 22.- Research Conference 2010 (GRCX) “Sequence-informed 24.11.2010. Crop Research”, Quedlinburg (Palais Salfeldt)/Gatersle- V244. Th i e l , T. & T. F. Sh a r b e l (vorgetragen von Th i e l , T.): Scruti- ben (IPK), 22.-24.11.2010. nizing gene copy number variation (CNV) as a factor for V230. Sh a r m a , R., R.K. Pa s a m , B. Kilian & A. Gr a n e r (vorgetragen apomixis expression in Boechera. – 9th IMPRS Sympo- von Sh a r m a , R.): Genome-wide association studies for sium, Dornburg, 15.02.2010. plant architecture traits in barley. – 6th Plant Science V245. v o n Wi r é n , N.: Ammonium sensing in Arabidopsis. – Student Conference, IPK, Gatersleben, 15.-18.06.2010. Molekulare Pflanzenphysiologie, Friedrich-Alexander- V231. Sreenivasulu , N.: Molecular basis of terminal drought tol- Universität Erlangen-Nürnberg, 07.01.2010. erance. – The University of Western Australia, School of V246. v o n Wi r é n , N.: Nitrogen sensing and signalling in plants. Plant Biology, Perth/Australia, 08.02.2010. – Wissenschaftszentrum Weihenstephan für Ernährung, V232. Sreenivasulu , N.: Integrated view of grain development Landnutzung und Umwelt, Technische Universität and deciphering the holistic view of reprogrammed München, 21.01.2010. seed metabolism under terminal drought. – ARC Excel- V247. v o n Wi r é n , N.: Ansätze aus der Pflanzenernährung zur lent Centre of Energy, Perth/Australia, 12.02.2010. genetischen Anpassung von Kulturpflanzen an den V233. Sreenivasulu , N., C. Se i l e r , V. Harshavadhan , K. Ra j e s h , Klimawandel. – Gesellschaft für Pflanzenzüchtung/ S. Wo r c h , M.S. Rö d e r , H. Ro l l e t s c h e k , J. Ku m l e h n , N. St a r o s k e , Deutsche Landwirtsch. Gesellschaft, Bonn, 01.03.2010. U. Co n r a d , W. We s c h k e , A. Bö r n e r , M. St r i c k e r t , K. Pi l l e n , L. V248. v o n Wi r é n , N.: Chancen und Risiken der grünen Gentech- Ku n t z e , V. Ko r z u n , B. Us a d e l , R. Su l p i c e , M. St i t t & U. Wo b u s nik. – Lion’s Club, Quedlinburg, 18.03.2010. (vorgetragen von Sreenivasulu , N.): SEEDs for the FUTURE: V249. v o n Wi r é n , N.: Ammonium transport and sensing in developing barley grains with improved yield and grain plants. – Botanisches Institut und Botanischer Garten, quality under terminal drought during seed filling. – Christian-Albrechts-Universität zu Kiel, 07.06.2010. 10th GABI Status Seminar, Potsdam, 09.-11.03.2010. V250. We b e r , H. & W. We s c h k e (vorgetragen von We b e r , H.): Same- V234. Sreenivasulu , N.: SEEDs for the FUTURE: Strategies for im- nentwicklung in Weizen. – GFP Sommertagung Get- proved seed yield and quality under terminal drought reide, IPK, Gatersleben, 09.-10.06.2010. in barley. – Martin-Luther-Universität Halle-Wittenberg, V251. We i c h e r t , N., A. Ha n e m a n n , H. Si m k o v a , J. Do l e z e l & M. S. Interdisziplinäres Zentrum für Nutzpflanzenforschung, Rö d e r (vorgetragen von Rö d e r , M. S.): Exploiting the Halle/S., 11.06.2010. genomic sequences of rice and Brachypodium for de- V235. Sreenivasulu , N.: Systems biology of seed metabolism for limiting a grain size QTL in wheat. – 10th Gatersleben improving yield stability. – National Institute of Plant Research Conference 2010 (GRCX) “Sequence-informed Genome Research, New Delhi/India, 10.12.2010. Crop Research”, Quedlinburg (Palais Salfeldt)/Gatersle- V236. St e i n , N.: Aktueller Stand der Weizengenomsequen- ben (IPK), 22.-24.11.2010. zierung und Perspektiven. – DLG-/GPZ-/ GFP-Sympo- V252. We i e r , D., F. Bo l l e n b e c k , R. Pi e l o t , U. Se i f f e r t & W. We s c h k e sium: Ertragsbildung bei Weizen, Gustav-Stresemann- (vorgetragen von We i e r , D.): A 4D atlas of the develop- Institut, Bonn, 01.-02.03.2010. ing barley grain. – International Conference “Molecular V237. St e i n , N.: How to sequence a 5 Gigabase genome – a Aspects of Plant Development”, Vienna/Austria, 23.- case study of barley (Hordeum vulgare). – Lunchtime 26.02.2010. seminar, Justus-Liebig-Universität, Gießen, 05.05.2010. V253. We i s e , S.: Novel developments of the MetaCrop infor- V238. St e i n , N.: Sequencing the 5 Gigabase barley genome ‑ mation system for facilitating systems biological ap- new perspectives for functional genomics in an import- proaches. – 6th International Symposium on Integrative ant cereal model and crop plant. – Seminar, Humboldt- Bioinformatics 2010, Cambridge/UK, 22.-24.03.2010. Universität zu Berlin, 11.05.2010. V254. We s c h k e , W.: Molekulare Schalter der Ertragsbildung bei V239. St e i n , N.: New achievements in Crop Genomics – or how Weizen. – DLG-/GPZ-/GFP-Symposium: Ertragsbildung to unlock the 5 Gigabase barley genome. – SFB Semi- bei Weizen, Gustav-Stresemann-Institut, Bonn, 01.- nar, Universität Würzburg, 27.05.2010. 02.03.2010. V240. St e i n , N.: How to sequence a 5 Gigabase genome – a case V255. We s c h k e , W.: 3-D-Mikrodissektion biologischer Objekte. – study of barley (Hordeum vulgare). – Seminary at Trait- BMBF-Projektforum Biotechnica, Hannover, 05.10.2010. Genetics 10th Anniversary, Gatersleben, 07.-08.06.2010. V256. Wi l l n e r, E.: Forage grasses – Poa central crop database V241. St e i n , N.: The best-guess barley genome – one step away development and assigning most original samples to- from barley whole genome sequencing. – Institutstag wards defining an European Forage Collection (EFC). – IPK, Gatersleben, 04.-05.10.2010. Baltic Sea Network for Management and Conservation V242. St e i n , N.: Barley – get ready for genomics based breed- of Plant Genetic Resources, Tallinn/Estonia, 30.11.2010. ing! – Genomics-based breeding, Justus-Liebig-Univer- sität, Gießen, 26.-28.10.2010.

177 Vorträge und Poster/Lectures and Posters

V257. Wi t z e l , K., A. Bö r n e r , J. Ku m l e h n , G. Ku n z e , M. Me l z e r , T. in developing ovules. – Plant and Animal Genome XIX. Ru t t e n , K.H. Mü h l i n g , M. St r i c k e r t , A. We i d n e r & H.-P. Mo c k Conference, San Diego/USA, 15.-19.01.2011. (vorgetragen von Wi t z e l , K.): Investigation of salinity V8. Am i t e y e , S., H. Vo g e l & T. F. Sh a r b e l (vorgetragen von stress responses in barley using the genetic variation of Am i t e y e , S.): Apomixis and hybridization in the genus mapping populations. – Gordon Res. Conference: Salt & Boechera: miRNA influences on global gene expres- Water Stress in Plants, Les Diablerets/Switzerland, 13.- sion patterns in developing ovules. – EU COST Apo- 18.06.2010. mixis Workshop, Valencia/Spain, 04.-05.07.2011. V258. Wo b u s , A.M.: Embryonale Stammzellen und Analyse der V9. Ba n a e i Mo g h a d d a m , A.M.: Directed nondisjunction of rye pankreatischen Entwicklung in vitro. – 527. Sitzung der B-chromosomes. – Japanese-German JSPS and DFG- Nordrhein-Westfälischen Akademie der Wissenschaften funded Workshop “Frontiers of Plant Chromosome Re- und Künste, Düsseldorf, 03.03.2010. search: Centromeres and Artificial Chromosomes”, IPK V259. Wo b u s , A.M.: Embryonale Stammzellforschung – woher Gatersleben, 31.10.-03.11.2011. und wohin? – Translationszentrum für Regenerative V10. Ba u e r , B.: Einfluss der N-Form auf Qualitätsparameter Medizin (TRM), Universität Leipzig, 11.03.2010. in Winterweizen. – Norddeutsches Marktfruchtforum, V260. Wo b u s , A.M.: Beziehung zwischen Pluripotenz und Tum- Kiel, 23.02.2011. origenität. – Novartis Ethical Advisory Board, Novartis V11. Ba u e r , B.: Perspectives of global wheat production. – International, Basel/Switzerland, 12.11.2010. agri benchmark Cash Crop Conference 2011, Middel- V261. Za y n a l i Ne z h a d , K., U. Lo h w a s s e r , M.S. Rö d e r & A. Bö r n e r fart, Copenhagen/Denmark, 12.-17.06.2011. (vorgetragen von Za y n a l i Ne z h a d , K.): Genetic linkage V12. Ba u e r , B.: Unterfußdüngung bei Minimalbodenbear- map construction and identification of quantitative beitung. Was ist machbar? Was ist erforderlich? – Fach- trait loci (QTLs) determining post-anthesis drought tol- hochschule Südwestfalen, Soest, 17.06.2011. erance and other agronomic traits in bread wheat. – 6th V13. Ba u e r , B.: Genotypical differences in the phytohormon- Plant Science Student Conference, IPK, Gatersleben, al response of wheat to nitrogen fertilisation. – ADAS, 15.-18.06.2010. Cambridge/UK, 06.09.2011. V262. Za y n a l i Ne z h a d , K.: Genetic linkage map construction and V14. Ba u e r , B.: Influence of nitrogen forms on tillering, cyto- identification of TQ Ls determining post-anthesis drought kinin translocation and yield in cereal plants. – Gemein- tolerance and other agronomic traits in bread wheat. – same Tagung der Deutschen Gesellschaft für Pflanzen- Oberseminar Pflanzenzüchtung, Martin-Luther-Univer- ernährung und der Gesellschaft für Pflanzenbau, Kiel, sität Halle-Wittenberg, Halle/S., 08.07.2010. 27.-29.09.2011. V15. Bä u m l e i n , H.: Identification and genetic analysis of the apospory locus in Hypericum perforatum. – Plant and 2011 Animal Genome XIX. Conference, San Diego/USA, 15.- 19.01.2011. V1. Al l a m , M.: The onset of a study in oilseed rape. – 7th V16. Bä u m l e i n , H.: Identification and genetic analysis of the Plant Science Student Conference, IPB, Halle/S., 14.- apospory locus in Hypericum perforatum. – Institutstag 17.06.2011. IPK, Gatersleben, 04.-06.10.2011. V2. Al t m a n n , T.: Hybridzüchtung: Heterotische Gruppen. – V17. Be n o r , S. & F. R. Bl a t t n e r (vorgetragen von Be n o r , S.): Phylo- Fachgespräch Weizen-Hybridzüchtung, BMELV, Bonn, geny and biogeography of the jute genus Corchorus (Mal- 09.02.2011. vaceae s.l.). – BioSystematics, Berlin, 21.-27.02.2011. V3. Al t m a n n , T.: PLANT-KBBE: CornFed – integration of ad- V18. Bo r i s j u k , L.: Unravelling developmental and positional cues vanced mapping and phenotyping methods to iden- for oil storage in rapeseed by 3D-NMR imaging. – Gordon tify key alleles for building European maize ideotypes. Research Conference on Plant Lipids: Structure, Metabo- – 11th GABI Status Seminar, Potsdam, 15.-17.03.2011. lism & Function, Galverston/USA, 30.01.-04.02.2011. V4. Al t m a n n , T.: Phänotypisierung – neue Verfahren für V19. Bo r i s j u k , L.: Dynamic 13C/1H NMR imaging uncovers die Erfassung von Pflanzeneigenschaften. – FNR/GFP sugar allocation in the living seed. – 10th International Strategiedialog II, Pflanzenzüchtung für nachwach- Conference of the International Society for Seed Science sende Rohstoffe, Berlin, 25.03.2011. (ISSS), Costa do Sauípe, Bahia/Brazil, 10.-15.04.2011. V5. Al t m a n n , T.: Analysis of natural variation and heterosis V20. Bö r n e r , A.: The German ex situ genebank in Gatersleben: of growth and metabolism in Arabidopsis and Maize. – Conservation and utilisation of plant genetic resourc- Institutstag IPK, Gatersleben, 04.-06.10.2011. es. – Seminar, USDA-ARS, National Center for Genetic V6. Al t m a n n , T.: Zugang zu neuen FuE-Konzepten durch Resources Preservation, Fort Collins, Colorado/USA, innovative Verfahren der Pflanzenphänotypisierung. 13.01.2011. – 1. Symposium Zierpflanzenzüchtung, Julius Kühn- V21. Bö r n e r , A.: Conservation and utilisation of plant genetic Institut, Quedlinburg, 15.-16.11.2011. resources in the German ex situ genebank in Gatersle- V7. Am i t e y e , S., H. Vo g e l & T. F. Sh a r b e l (vorgetragen von Am i t e y e , ben. – National Bureau of Plant Genetic Resources, New S.): Apomixis and hybridization in the genus Boechera: Delhi/India, 01.03.2011. miRNA influences on global gene expression patterns

178 V22. Bö r n e r , A., M.A. Re h m a n Ar i f , K. Ne u m a n n , M. Na g e l , U. Lo h - V34. Cz a u d e r n a , T. & F Sc h r e i b e r (vorgetragen von Sc h r e i b e r , F.): w a s s e r & B. Ko b i l j s k i (vorgetragen von Bö r n e r , A.): Associa- SBGN-ED – working with the Systems Biology Graphical tion genetics – a new strategy to study seed and seed- Notation. – COMBINE 2011, Heidelberg, 03.-07.09.2011. ling stress tolerance in crop plants. – 10th International V35. Da g h m a , D.S.: Structural changes during the initiation Conference of the International Society for Seed Science of pollen embryogenesis in barley. – International PhD (ISSS), Costa do Sauípe, Bahia/Brazil, 10.-15.04.2011. School on Plant Development, Retzbach-Würzburg, V23. Bö r n e r , A., E.K. Kh l e s t k i n a , T.A. Pshenichnikova , S. Ch e b o t a r , 05.-07.10.2011. M. Na g e l , M.A. Re h m a n Ar i f , K. Ne u m a n n , B. Ko b i l j s k i , U. Lo h - V36. De h m e r , K.J.: Diversity analyses in wild potatoes via mul- w a s s e r & M. S. Rö d e r (vorgetragen von Bö r n e r , A.): Main- tiplex fluorescent SSR fingerprints. – th18 Triennial Con- tenance and utilisation of wheat genetic resources for ference of the European Association for Potato Research future breeding. – International Conference “Wheat (EAPR2011), Oulu/Finland, 24.-29.07.2011. Genetic Resources and Genomics”, Novosibirsk/Russia, V37. De m i d o v , D.: Consequences of spindle checkpoint mis- 28.08.-01.09.2011. function in plants by modulation of AtAurora activity. V24. Bö r n e r , A., E.K. Kh l e s t k i n a , T.A. Pshenichnikova , K. Ne u m a n n , – Japanese-German JSPS and DFG-funded Workshop U. Lo h w a s s e r , B. Ko b i l j s k i , V. Ko r z u n & M. S. Rö d e r (vorge- “Frontiers of Plant Chromosome Research: Centromeres tragen von Bö r n e r , A.): Cereal genetic stocks – examples and Artificial Chromosomes”, IPK Gatersleben, 31.10.- of successful co-operation. – EWAC-EUCARPIA Cereals 03.11.2011. Section Conference, Novi Sad/Serbia, 07.-11.11.2011. V38. Di e k m a n n , K.: Illustration of the diversity of genebank V25. Br a s s a c , J., S.S. Ja k o b & F. R. Bl a t t n e r (vorgetragen von accessions of cultivated potato using simple sequence Br a s s a c , J.): Analysis of phylogenetic relationships of repeat markers. – 18th Triennial Conference of the Euro- Hordeum (Poaceae) polyploids. – BioSystematics, Berlin, pean Association for Potato Research (EAPR2011), Oulu/ 21.-27.02.2011. Finland, 24.-29.07.2011. V26. Bu d a h n , H., T. Br u c h m ü l l e r , D. Ul r i c h , H. Kr ü g e r , U. Lo h w a s s e r & F V39. Dobrovolskaya , O., J. Sa l s e , P. Ma r t i n e k , V.S. Ar b u z o v a , O.M. Ma r t h e (vorgetragen von Bu d a h n , H .): Charakterisierung der Po p o v a , A. Bö r n e r , E.A. Sa l i n a & L. I. La i k o v a (vorgetragen intraspezifischen Variabilität bei Petersilie mittels moleku- von Dobrovolskaya , O.): Characterization of genes that larer Marker sowie klassischer und nicht-zielgerichteter are involved in control of spike development in wheat Bestimmung flüchtiger Inhaltsstoffe. – 6. Fachtagung Arz- and its relatives. – International Conference “Wheat nei- und Gewürzpflanzen, Berlin, 19.-22.09.2011. Genetic Resources and Genomics”, Novosibirsk/Russia, V27. Co n r a d , U.: Production of very large spider silk proteins by 28.08.-01.09.2011. posttranslational fusions in vivo. – COST Meeting Cost Ac- V40. Fi n k e , A., M. Ku h l m a n n & M. F. Me t t e (vorgetragen von tion Molecular Farming, Ghent/Belgium, 14.-16.09.2011. Fi n k e , A.): Genetic analysis of RNA-directed transcrip- V28. Co n r a d , U.: Production and characterization of large tional gene silencing in Arabidopsis. – 7th Plant Science spider silk protein multimers in transgenic tobacco by Student Conference, IPB, Halle/S., 14.-17.06.2011. posttranslational fusions. – Institutstag IPK, Gatersle- V41. Fi n k e , A., M. Ku h l m a n n & M. F. Me t t e (vorgetragen von Fi n k e , ben, 04.-06.10.2011. A.): Genetic analysis of RNA-directed transcriptional V29. Co n r a d , U.: Production of therapeutically and technically gene silencing in Arabidopsis. – 2nd European Workshop important proteins in plants: avian flu antigens and spi- on Plant Chromatin, Versailles/France, 01.-02.09.2011. der silk proteins from tobacco. – Kolloquium, Institut für V42. Fl e i s c h e r , F., C. Vo l k m a r & A. Bö r n e r (vorgetragen von Biochemie II, Universität Düsseldorf, 19.10.2011. Fl e i s c h e r , F.): Untersuchungen eines Winterweizensorti- V30. Co n r a d , U.: Production and immunological characteriza- ments 2011 am Standort Gatersleben auf Befall durch tion of avian flu hemagglutinin multimers in transgenic Weizengallmücken. – Arbeitskreis „Populationsdynamik plants. – Pharma Planta Open Meeting, Brussels/Bel- und Epidemiologie”, Martin-Luther-Universität Halle- gium, 25.10.2011. Wittenberg, Halle/S., 22.09.2011. V31. Co n r a d , U.: Production of spider silk derivatives in trans- V43. Fl o r s c h ü t z , K., A. Sc h r ö t e r , K. Ve t t e r , R. Wa t z k e & G. Ku n z e genic plants: purification and characterization. – Kick- (vorgetragen von Fl o r s c h ü t z , K.): Nachweis und Quan- Off Meeting COST-Action Netzwerk “Biological Adhe- tifizierung von arbuskulären Mykorrhizapilzen. – 15. sives: From Biology to Biomimetics”, Vienna/Austria, Jahrestagung der DPG-Projektgruppe Mikrobielle 03.-05.11.2011. Symbiose des Arbeitskreises Phytomedizin in Garten- V32. Cz a u d e r n a , T., M.P. v a n Ie r s e l & A. C. Vi l l e g e r (vorgetragen bau und Forst und der Vereinigung der Mykorrhizaan- von Cz a u d e r n a , T.): An introduction to SBGN-ML. – HAR- wender und -produzenten Deutschlands, Lüneburg, MONY 2011: The Hackathon on Resources for Modeling 24.-25.11.2011. in Biology, New York/USA, 18.-22.04.2011. V44. Fr a n k e , M., J. Hu e g e , C. Kr a c h , H. Po s g a r & B. H. Ju n k e r (vor- V33. Cz a u d e r n a , T. & F Sc h r e i b e r (vorgetragen von Sc h r e i b e r , F.): getragen von Fr a n k e , M.): Metabolic flux analysis in bar- Using SBGN-ED for the Systems Biology Graphical No- ley. – 7th Plant Science Student Conference, IPB, Halle/S., tation. – International German/Russian Summer School 14.-17.06.2011. in “Integrative Biological Pathway Analysis and Simula- V45. Fr i e d e l , S.: What can genebank data tell us? – Institutstag tion”, Bielefeld, 04.-07.07.2011. IPK, Gatersleben, 04.-06.10.2011.

179 Vorträge und Poster/Lectures and Posters

V46. Fr i t s c h , R.M.: Iran - terra incognita Alliorum? – Insti- of Genetic Engineering and Biotechnology, Tehran/Iran, tutskolloquium, Conservatoire et Jardin botaniques 14.-16.12.2011. ville de Genève, Geneva/Switzerland, 20.10.2011. V64. Ha r p k e , D., H. Ke r n d o r f f , T. Ru t t e n & F. R. Bl a t t n e r (vorge- V47. Gi e r s b e r g , M.: Nachweis östrogener Aktivitäten mittels tragen von Ha r p k e , D.): Two times into Asia Minor: phy- A-YES Assay. – Arbeitskreis „Endokrine Wirkungen” – 2. logeny of Crocus (Iridaceae) based on ITS sequences of Sitzung, Bundesanstalt für Gewässerkunde, Koblenz, nuclear rDNA. – BioSystematics, Berlin, 21.-27.02.2011. 22.03.2011. V65. He c k m a n n , S., I. Le r m o n t o v a , D. De m i d o v , B. Be r c k m a n s , L. d e V48. Gi l s , M.: Hybridzüchtung in Weizen. – Innoplanta Jahres- Ve y l d e r , H. Bä u m l e i n , I. Sc h u b e r t & A. Ho u b e n (vorgetragen tagung, Gatersleben, 14.03.2011. von He c k m a n n , S.): Transcriptional and posttranslational V49. Gi l s , M.: Pollination control systems for hybrid wheat breed- regulation of the Aurora-substrate CENH3 in Arabidop- ing. – German Seed Alliance, Isernhagen, 13.04.2011. sis. – SFB 648 Meeting, Martin-Luther-Universität Halle- V50. Gi l s , M.: Pollination control systems for hybrid wheat Wittenberg, Halle/S., 04.02.2011. breeding. – Universität Hohenheim, 21.06.2011. V66. He c k m a n n , S., I. Le r m o n t o v a , D. De m i d o v , B. Be r c k m a n s , L. d e Ve y l - V51. Gi l s , M.: Pollination control systems for hybrid wheat d e r , H. Bä u m l e i n , I. Sc h u b e r t & A. Ho u b e n (vorgetragen von breeding. – Visit European Plant Breeding Academy UC He c k m a n n , S.): Transcriptional and posttranslational regu- Davis, IPK Gatersleben, 23.06.2011. lation of the Aurora-substrate CENH3 in Arabidopsis. – V52. Gi l s , M.: Pollination control systems for hybrid wheat DST-DAAD Project Based Personal Exchange Programme, breeding. – Institutstag IPK, Gatersleben, 04.-06.10.2011. University of Jammu/India, 16.03.2011. V53. Gi l s , M.: Mit geteilten Genen zum vollen Ertrag – neue V67. He c k m a n n , S.: Holocentric chromosome organization and Wege in der Weizen-Hybridzucht. – 22. Meeting of Trust- behaviour during mitosis and meiosis in Luzula elegans. – ees (Stiftungsrat), IPK Gatersleben, 07.10.2011. Japanese-German JSPS and DFG-funded Workshop “Fron- V54. Gi l s , M.: Etablierung eines innovativen Systems zur Her- tiers of Plant Chromosome Research: Centromeres and Ar- stellung von Hybridweizen. – BMBF-Projektforum, Bio- tificial Chromosomes”, IPK Gatersleben, 31.10.-03.11.2011. technica Hannover, 11.10.2011. V68. He d t m a n n , C., A. Ma t r o s & H. P. Mo c k (vorgetragen von V55. Gr a n e r , A.: Genomforschung zur Nutzbarmachung pflan- He d t m a n n , C.): Proteomic approaches to investigate the zengenetischer Ressourcen. – Institutskolloquium, Chris- regulation of stress-inducible proteins from Arabidopsis tian-Albrechts-Universität, Kiel, 12.01.2011. by phosphorylation. – Meeting COST Action FA0603, Di- V56. Gr a n e r , A.: Von der Natur zur Kultur: Nutzpflanzen als jon/France, 25.-27.05.2011. Lebensgrundlage des Menschen. – Institutskolloquium, V69. He n s e l , G., C. Be r g e r , S. Bi e r i , C. Bo l l m a n n , A. Br u c h m ü l l e r , Technische Universität Ilmenau, 26.01.2011. D. Do u c h k o v , S. Fr i e d e l , S. Go e d e k e , E. Gr ü t z e m a n n , M. Gu r u - V57. Gr a n e r , A.: Podiumsdiskussion: “Genomics, untapped bio- s h i d z e , A. Hi m m e l b a c h , S. Lü c k , C. Ma r t h e , A. Mü l l e r, I. Ot t o , J. diversity, GMOs and socio-economic concerns”. – 15th An- Ri e c h e n , P. Sc h w e i z e r & J. Ku m l e h n (vorgetragen von He n s e l , nual ADNAT Convention, Intl. Symposium on Genomics G.): Engineered minichromosomes as vectors in barley. and Biodiversity, Hyderabad/India, 23.-25.02.2011. – International Conference “Plant Transformation Tech- V58. Gr a n e r , A.: Towards a genomics driven valorization of nologies II”, Vienna/Austria, 19.-22.02.2011. plant genetic resources. – Annual Symposium of the V70. He n s e l , G.: Expression systems for the production of Otto Warburg Centre for Agricultural Biotechnology, pharmaceutical or technical proteins in barley. – Gen- Golm, 14.04.2011. technik-Workshop der Gesellschaft für Pflanzenbiotech- V59. Gr a n e r , A.: Grüne Gentechnik – Wahrnehmung und nologie, Quedlinburg und Üplingen, 22.-23.08.2011. Realität. – Jahrestreffen ehemaliger Absolventen der V71. He n s e l , G.: Genetic engineering in barley – methods and Ingenieurswissenschaften der Leibniz-Universität Han- applications. – Japanese-German JSPS and DFG-funded nover, Quedlinburg, 07.05.2011. Workshop “Frontiers of Plant Chromosome Research: V60. Gr a n e r , A.: Genomics informed valorization of plant ge- Centromeres and ArtificialC hromosomes”, IPK Gatersle- netic resources. – Seminarvortrag, National Institute ben, 31.10.-03.11.2011. for Plant Genomics Research (NIPGR), New Delhi/India, V72. Ho e r a n d l , E., D. Ho j s g a a r d , T.F. Sh a r b e l , M. Pe l l i n o & H. Vo g e l 18.10.2011. (vorgetragen von Ho e r a n d l , E.): Effects of hybridization V61. Gr a n e r , A.: Grüne Gentechnik – Rolle der Pflanzenbio- on the expression of apomixis in Ranunculus auricomus. technologie für die Ernährungssicherung. – Future of – Plant and Animal Genome XIX. Conference, San Die- Food, Hochschule Anhalt, Bernburg, 26.10.2011. go/USA, 15.-19.01.2011. V62. Ha j i r e z a e i , M.R.: Microarray-based identification of genes V73. Ho j s g a a r d , D., H. Vo g e l , T.F. Sh a r b e l , M. Pe l l i n o & E. Ho e r a n d l differentially expressed in stem base of Petunia hybrida (vorgetragen von Ho j s g a a r d , D.): Hybridization delays in different developmental stages of adventitious root the sexual development and triggers apomixis in the formation. – 6th International Symposium on Root De- Ranunculus auricomus complex. – EU COST Apomixis velopment, Amos/Canada, 07.-11.08.2011. Workshop, Valencia/Spain, 04.-05.07.2011. V63. Ha j i r e z a e i , M.R.: Improvement of agronomical impor- V74. Ho u b e n , A.: Histone phosphorylation – a dynamic affair. tant crops by understanding the underlying biological – Department of Plant Biochemistry, Polish Academy of mechanisms. – Symposium on Stress, National Institute Sciences, Warsaw/Poland, 17.05.2011.

180 V75. Ho u b e n , A.: Role of CENH3 in stable and unstable grass V88. Ke l l e r , E.R.J.: Veranstaltungen und Initiativen zur Kryo- hybrids. – Japanese-German JSPS and DFG-funded konservierung 2010 / 2011: COST 871 – Abschlussta- Workshop “Frontiers of Plant Chromosome Research: gung in Angers, EU-Projekt-Abschlusstagung in Prag Centromeres and ArtificialC hromosomes”, IPK Gatersle- und Erstes Internationales Kryo-Symposium in Yangling, ben, 31.10.-03.11.2011. China. – Jahrestagung des Arbeitskreises Deutsche In- V76. Ju n k e r , B.H.: Integrated analysis and modeling of legume vitro-Kulturen ADIVK, Bremen, 15.-16.09.2011. seed metabolism. – FORSYS Young Investigator Semi- V89. Kh l e s t k i n a , E.K., O.Y. Tereshchenko , V.S. Ar b u z o v a , A. Bö r n e r , nar, Heidelberg, 14.02.2011. L.A. Pe r s h i n a & E.A. Sa l i n a (vorgetragen von Kh l e s t k i n a , V77. Ju n k e r , B.H.: Analysis and modeling of metabolic fluxes E.K.): A new range of wheat precise genetic stocks ap- in plants. – Institut für Pharmazie, Martin-Luther-Uni- plication: insights into gene function. – EWAC-EUCAR- versität Halle-Wittenberg, Halle/S., 27.04.2011. PIA Cereals Section Conference, Novi Sad/Serbia, 07.- V78. Ju n k e r , B.H.: Metabolic flux analysis in plant seeds – at 11.11.2011. the interface between metabolomics and systems bi- V90. Kilian, B.: Genetic diversity and domestication of wheat ology. – Max-Planck-Institut für Pflanzenzüchtungsfor- and barley. – Martin-Luther-Universität Halle-Witten- schung, Köln, 06.07.2011. berg, Halle/S., 15.07.2011. V79. Ju n k e r , B.H.: Towards high-throughput fluxomics in crop V91. Ki t t l e r , J., H. Kr ü g e r , W. Sc h ü t z e , U. Kä s t n e r , W. Ju n g h a n n s , seeds. – Institutstag IPK, Gatersleben, 04.-06.10.2011. W.D. Bl ü t h n e r , U. Lo h w a s s e r & F Ma r t h e (vorgetragen von V80. Ka p u s i , E., C.H. Te o , L. Ma, G. He n s e l , J. Ku m l e h n , I. Sc h u b e r t , Ki t t l e r , J.): Charakterisierung unterschiedlicher Gen- M.F. Me t t e & A. Ho u b e n (vorgetragen von Ka p u s i , E.): Engi- pools der Melisse (Melissa officinalis) als Basis für die neered minichromosomes as vectors in barley. – Inter- Entwicklung von züchterisch wertvollem Ausgangs- national Conference “Plant Transformation Technolo- material. – 6. Fachtagung Arznei- und Gewürzpflanzen, gies II”, Vienna/Austria, 19.-22.02.2011. Berlin, 19.-22.09.2011. V81. Ka r i m i As h t i y a n i , R.: AtHaspin phosphorylates histone H3 V92. Kl u k a s , C.: Automated plant phenotyping at the IPK. at threonine 3 and contributes to embryonic patterning – College of Life Sciences, Zhejiang University, Hang- in Arabidopsis. – Japanese-German JSPS and DFG-fund- zhou/China, 26.07.2011. ed Workshop “Frontiers of Plant Chromosome Research: V93. Kl u k a s , C.: Development of an information system for Centromeres and ArtificialC hromosomes”, IPK Gatersle- automated crop plant phenotyping. – Institutstag IPK, ben, 31.10.-03.11.2011. Gatersleben, 04.-06.10.2011. V82. Ke l l e r , E.R.J., H.M. Sc h u m a c h e r , M. Hö f e r , A. Me i e r -Di n k e l , V94. Kn ü p f f e r , H.: Chair’s report: activities and achievements K. Zo g l a u e r & I. Pi n k e r (vorgetragen von Ke l l e r , E.R.J.): of the Working Group on Barley since its 6th meeting Country Report: Cryopreservation in Germany. – Final (Salsomaggiore, Italy, 2000) and the 2nd meeting of the Meeting of COST 871 CryoPlanet, Angers/France, 08.- Cereals Network (Foça, Turkey, 2008). – 7th Meeting of 11.02.2011. the ECPGR Barley Working Group, Nicosia/Cyprus, 10.- V83. Ke l l e r , E.R.J., A. Se n u l a , C. Za n k e , M. Gr ü b e , A. Ka c z m a r c z y k , 12.05.2011. A. Nu k a r i , D. Te y s s e d r e , C. Kr e m e r Mo r a l e s , J. Ed e s i , M. Pe l c V95. Kn ü p f f e r , H.: Hordeum wild species in Europe – crop wild & M. Ol a s -So c h a c k a (vorgetragen von Ke l l e r , E.R.J.): Ways relatives of cultivated barley. – 7th Meeting of the ECPGR of collaboration – COST short-term scientific missions Barley Working Group, Nicosia/Cyprus, 10.-12.05.2011. on three crops and their outcomes – potato, garlic and V96. Kn ü p f f e r , H.: European Barley Database and EURISCO, mint. – Final Meeting of COST 871 CryoPlanet, Angers/ International Barley Core Collection. – 7th Meeting of France, 08.-11.02.2011. the ECPGR Barley Working Group, Nicosia/Cyprus, 10.- V84. Ke l l e r , E.R.J.: Formalization of vegetatively propagated 12.05.2011. crop’s standards. – 7th Meeting of the ECPGR Allium Work- V97. Kn ü p f f e r , H.: Rye genetic resources in the world’s gene- ing Group, Perea, Thessaloniki/Greece, 06.-08.09.2011. banks. – AEGIS Workshop “Improving the prerequisites V85. Ke l l e r , E.R.J.: Future development and project ideas for a European rye collection”, Radzików/Poland, 13.- for vegetatively propagated alliums. – 7th Meeting of 14.10.2011. the ECPGR Allium Working Group, Perea, Thessaloniki/ V98. Ko h l , S.: Relationship between senescence-induced N- Greece, 06.-08.09.2011. remobilization and seed filling in barley. – Meeting DFG- V86. Ke l l e r , E.R.J. & C. Za n k e (vorgetragen von Ke l l e r , E.R.J.): Forschergruppe FOR948 „Nitrogen uptake, metabolism The AEGIS Project “Cryopreservation of young inflores- and remobilization in leaves during plant senescence”, cence bases in bolting garlic for germplasm storage“. – Graz/Austria, 12.-13.05.2011. 7th Meeting of the ECPGR Allium Working Group, Perea, V99. Ku m l e h n , J.: Genetische Transformation bei Getreide. – 2. Thessaloniki/Greece, 06.-08.09.2011. Quedlinburger Pflanzenzüchtungstage, Quedlinburg, V87. Ke l l e r , E.R.J. & C. Za n k e (vorgetragen von Ke l l e r , E.R.J.): 29.-30.03.2011. EURALLIVEG – vegetative Allium, Europe’s core collec- V100. Ku m l e h n , J.: Genetic engineering and haploid technol- tion, safe and sound. – 7th Meeting of the ECPGR Al- ogy in cereals. – University of Cape Town, Cape Town/ lium Working Group, Perea, Thessaloniki/Greece, 06.- South Africa, 13.09.2011. 08.09.2011.

181 Vorträge und Poster/Lectures and Posters

V101. Ku m l e h n , J.: Investigations on the initiation of pollen V114. Lo h w a s s e r , U.: The rye collection of the German genebank. embryogenesis. – Institutstag IPK, Gatersleben, 04.- – Workshop “Improving the prerequisites for a European 06.10.2011. rye collection”, Radzików/Poland, 13.-14.10.2011. V102. Ku n z e , G.: Arxula adeninivorans – a valuable tool for basic V115. Ma, L.: Generation of engineered chromosomes in bar- research and biotechnological application. – Kolloqui- ley. – Japanese-German JSPS and DFG-funded Workshop um, IPK Gatersleben, 27.04.2011. “Frontiers of Plant Chromosome Research: Centromeres V103. Ku n z e , G.: Glomus irregulare AMykor – a molecular ap- and Artificial Chromosomes”, IPK Gatersleben, 31.10.- proach to characterize and identify the arbuscular 03.11.2011. mycorrhizal fungus. – Institutstag IPK, Gatersleben, 04.- V116. Ma r c o n i , G., G. Ga l l a , M. Co n n e r , L. Ra g i , G. Ba r c a c c i a , P. Oz i a s - 06.10.2011. Ak i n s , T.F. Sh a r b e l , M. Fa l c i n e l l i & E. Al b e r t i n i (vorgetragen V104. La n g e , M.: The LAILAPS information retrieval portal as von Ma r c o n i , G.): Cross-species characterization of APO- scalable and integrative database query endpoint. – START for unveiling its role in apomixis. – EU COST Action Workshop “Translational Bioinformatics” of 6th Interna- Apomixis Workshop, Valencia/Spain, 04.-05.07.2011. tional Symposium on Health Informatics and Bioinfor- V117. Ma r t í n , C., A. Se n u l a , I. Go n z á l e z , A. Ac o s t a , M.E. Go n z á l e z - matics (HIBIT2011), Izmir/Turkey, 02.-05.05.2011. Be n i t o & E.R.J. Ke l l e r (vorgetragen von Ma r t í n , C.): The role V105. Le r m o n t o v a , I.: Centromere maintenance via CENH3. – of cryopreservation in the long-term conservation of Japanese-German JSPS and DFG-funded Workshop vegetatively propagated plants. – Final Meeting of COST “Frontiers of Plant Chromosome Research: Centromeres Action 871 CryoPlanet, Angers/France, 08.-11.02.2011. and Artificial Chromosomes”, IPK Gatersleben, 31.10.- V118. Ma s c h e r , M., U. Sc h o l z & S. Fr i e d e l (vorgetragen von 03.11.2011. Ma s c h e r , M.): Chargaff’s second parity rule in plant ge- V106. Liiving, T. & B. H. Ju n k e r (vorgetragen von Liiving, T.): In- nomes. – JCB Workshop “Nucleotides – Networks – Nov- vestigating the starch synthesis pathway for kinetic elties. Understanding Evolution Beyond Darwin and modeling in pea embryos during seed filling phase. – Haeckel”, Jena, 28.-29.03.2011. 7th Plant Science Student Conference, IPB, Halle/S., 14.- V119. Ma s c h e r , M.: Chargaff’s second parity rule in grass ge- 17.06.2011. nomes. – 7th Plant Science Student Conference, IPB, V107. Lo h w a s s e r , U., A. Di t t b r e n n e r , F. Ma r t h e & A Bö r n e r (vorge- Halle/S., 14.-17.06.2011. tragen von Lo h w a s s e r U.): Taxonomy of plant genetic re- V120. Ma s c h e r , M.: Chargaff’s NAD fragment assembly: an ap- sources – and interaction of morphological, molecular plication of graph theory in molecular biology. – Ober- and phytochemical data. – BioSystematics, Berlin, 21.- seminar Algebra, Fakultät für Mathematik, Otto-von- 27.02.2011. Guericke-Universität, Magdeburg, 05.07.2011. V108. Lo h w a s s e r , U., M. Na g e l & A Bö r n e r (vorgetragen von Lo h - V121. Ma t r o s , A., M. Pe u k e r t , S. Ka s p a r , W. We s c h k e & H. P. Mo c k w a s s e r , U.): Longevity of seeds – intraspecific differences (vorgetragen von Ma t r o s , A.): Spatial protein and me- in germplasm collections. – BioSystematics, Berlin, 21.- tabolite profiling during barley grain development by 27.02.2011. MALDI-MS imaging and LC-MS approaches. – DGMS- V109. Lo h w a s s e r , U.: The umbellifers (Apiaceae) of the German Tagung, Dortmund, 27.02.-02.03.2011. ex situ genebank. – ECPGR Umbellifer Working Group V122. Ma t r o s , A.: Analysis of spatial and temporal changes Meeting, Quedlinburg, 30.03.-01.04.2011. during barley grain development. – Charles University, V110. Lo h w a s s e r , U. & A. Bö r n e r (vorgetragen von Lo h w a s s e r , Prague/Czech Republic, 18.10.2011. U.): Quality standards in genebanks – improvement of V123. Me h l h o r n , H.: The DBE2 information system. – Work- sustainability of plant genetic resources. – EUCARPIA shop “Translational Bioinformatics” of 6th International Section Genetic Resources, European Plant Genetic Symposium on Health Informatics and Bioinformatics Resources Conference 2011, Wageningen/The Nether- (HIBIT2011), Izmir/Turkey, 02.-05.05.2011. lands, 05.-07.04.2011. V124. Me h l h o r n , H.: Integration of biological networks using V111. Lo h w a s s e r , U. & A. Bö r n e r (vorgetragen von Lo h w a s s e r , U.): VANTED NETS. – 7th Plant Science Student Conference, Genbanken – Ressourcen für künftige Generationen. – IPB, Halle/S., 14.-17.06.2011. Symposium on Biodiversität, Geistiges Eigentum und V125. Me h l h o r n , H.: Integration of biological networks using Innovation, Philipps-Universität Marburg, 20.05.2011. VANTED NETS. – International German/Russian Sum- V112. Lo h w a s s e r , U.: Sammlungen alter Kulturpflanzen – Gen- mer School in “Integrative Biological Pathway Analysis reservoir für die Zukunft? – Deutsche Biotechnologie- and Simulation”, Bielefeld, 04.-07.07.2011. tage 2011, München, 25.-26.05.2011. V126. Me h l h o r n , H.: Analysis of biological networks. – Interna- V113. Lo h w a s s e r , U., M.A. Re h m a n Ar i f , K. He r r m a n n & A Bö r n e r tional German/Russian Summer School in “Integrative (vorgetragen von Lo h w a s s e r , U.): New strategies for Biological Pathway Analysis and Simulation”, Bielefeld, discovery loci determining pre-harvest sprouting and 04.-07.07.2011. dormancy in wheat and barley. – The 12th International V127. Me h l h o r n , H. & F. Sc h r e i b e r (vorgetragen von Me h l h o r n , Symposium on Pre-Harvest Sprouting in Cereals, Red H.): Integration and exploration of biological networks. Deer, Alberta/Canada, 24.-27.07.2011. – Workshop „Datenmanagement in den Lebenswissen- schaften”, Berlin, 06.10.2011.

182 V128. Me l z e r , B., T. Gu s e , C. Ka s t n e r , K. Pl a s u n , C. Be r g e r , E. Ka p u s i , V141. Na g e l , M., I. Kr a n n e r & A. Bö r n e r (vorgetragen von Na g e l , G. He n s e l , C. Ma r t h e , P. Ho f f m e i s t e r & J. Ku m l e h n (vorge- M.): Biochemical and genetic studies on seed longevity tragen von Me l z e r , B.): Novel tools for Brachypodium rein the German ex situ genebank. – Seminar, Wagenin- search: Genetic transformation using shoot segments gen University and Research Centre (WUR), Wagenin- and immature pollen-derived generation of instantly gen/The Netherlands, 17.10.2011. homozygous lines. – 1st European Brachypodium Work- V142. Ne u m a n n , K.: Die Identifizierung von TQ Ls für die Trock- shop, Paris/France, 19.-21.10.2011. entoleranz in Gerste. – Oberseminar Pflanzenzüchtung, V129. Me l z e r , M.: The microworld of a plant cell: Microscopy Martin-Luther-Universität Halle-Wittenberg, Halle/S., techniques in plant research application. – Faculty of Bi- 10.02.2011. ology and Environment Protection, University of Silesia, V143. Ne u m a n n , K., A.F. Ba l i n t , F. Sz i r a , M. Ba u m , R.K. Va r s h n e y & A. Katowice/Poland, 19.05.2011. Bö r n e r (vorgetragen von Ne u m a n n , K.): Genome-wide as- V130. Me l z e r , M.: Cells in the focus: Microscopy in plant re- sociation mapping for early and late drought tolerance search. – Royal Institute of Technology (KTH), UAlbaNo- in a diverse barley collection. – Agrisafe Final Confer- va University Center, Stockholm/Sweden, 22.06.2011. ence “Climate change: challenge for training of applied V131. Me l z e r , M.: Comparative structural cell biological stud- plant scientists“, Budapest/Hungary, 21.-23.03.2011. ies of gametophytic pollen development vs. initiation V144. Ne u m a n n , K., B. Ko b i l j s k i , S. De n č i ć , R.K. Va r s h n e y & A. Bö r n e r of pollen embryogenesis in barley. – Institutstag IPK, (vorgetragen von Ne u m a n n , K.): Genome-wide asso- Gatersleben, 04.-06.10.2011. ciation mapping of agronomic traits in bread wheat. – V132. Mo c k , H.-P.: Proteomics to evaluate genetic resources EWAC-EUCARPIA Cereals Section Conference, Novi Sad/ for stress defence responses. – INRA, Clermont-Ferrand/ Serbia, 07.-11.11.2011. France, 24.05.2011. V145. Nü r k , N.M., S. Ur i b e -Co n v e r s , D. Ta n k , S. Ma d r i ñ á n , M. Ca r i n e , V133. Mo c k , H.-P.: Studying barley seed development by M. Ch a s e & F. R. Bl a t t n e r (vorgetragen von Nü r k , N.M.): spatially resolved mass spectrometrical analysis. – Molecular phylogeny and historical biogeography of Danish Technical University, Copenhagen/Denmark, Hypericum. – BioSystematics, Berlin, 21.-27.02.2011. 07.06.2011. V146. Nü r k , N.M.: Tracing the impact of the Andean uplift – V134. Mo c k , H.-P.: Analysis of salt stress tolerance mechanisms evolutionary history of Hypericum in South America. in barley mapping populations. – Institutstag IPK, – DFG Nachwuchsakademie “Systematik der Pflanzen Gatersleben, 04.-06.10.2011. und Pilze”, Frankfurt am Main, 25.05.2011. V135. Mo c k , H.-P.: Phytochemical analysis of anthocyanins. V147. Pe r u z z i , L., D. Ha r p k e & A. Ca r t a (vorgetragen von Pe r u z - – 1st Annual Meeting Athena, Acireale, Sicily/Italy, 12.- z i , L.): Risultati preliminari sull’evoluzione e filogenesi, 14.10.2011. nell’ambito della serie Verni (Crocus, Iridaceae), dei due V136. Mo c k , H.-P.: Proteomics to evaluate genetic resources crochi endemici di Toscana. – Riunione Annuale Grup- for stress defence responses. – KEW Botanical Gardens/ po Biosistematica 2011, Castiglioncello (Livorno)/Italy, Wakehurst Place, West Sussex/UK, 07.12.2011. 11.06.2011. V137. Mo s t e k , A., S. Lü t h j e , C. Me i s r i m l e r , A. Bö r n e r & A. We i d n e r V148. Pe t e r s o n , A. & D. Ha r p k e (vorgetragen von Pe t e r s o n , A.): (vorgetragen von Mo s t e k , A.): Proteome differences be- Molecular insights in the species-rich polyploid Lili- tween salt-sensitive and tolerant barley and alleviating aceae genus Gagea: impact of molecular data for deter- influence ofBABA . – Final COST Action Meeting FA0603 mination of hybridogenic taxa and species boundaries. ‘Plant Proteomics in Europe’, Dijon/France, 26.05.2011. – BioSystematics, Berlin, 21.-27.02.2011. V138. Na g e l , M. & A. Bö r n e r (vorgetragen von Na g e l , M.): Im- V149. Pe u k e r t , M., A. Ma t r o s & H. P. Mo c k (vorgetragen von Pe u - pacts of the genetic background on seed deterioration k e r t , M.): MALDI-imaging for spatial protein and me- in crops. – 10th International Conference of the Interna- tabolite profiling during barley grain development. – tional Society for Seed Science (ISSS), Costa do Sauípe, Bruker Anwendertreffen, Kassel, 21.-22.03.2011. Bahia/Brazil, 10.-15.04.2011. V150. Pistrick, K.: Das wissenschaftliche Werk von Paul Schuster V139. Na g e l , M., I. Kr a n n e r & A. Bö r n e r (vorgetragen von Na g e l , (1876–1965). – Eröffnung einer zusammen mit U. Kison M.): Conservation of germplasm collections – biochem- konzipierten und gestalteten Ausstellung „Paul Schus- ical and genetic impacts on seed ageing in barley. – 10th ter – Pfarrer und Botaniker in Meisdorf”, Patronatskirche International Conference of the International Society Meisdorf, Stadt Falkenstein/Harz, 27.08.2011. for Seed Science (ISSS), Costa do Sauípe, Bahia/Brazil, V151. Pshenichnikova , T.A., M.D. Pe r m y a k o v a , S.V. Os i p o v a , A.V. Pe r - 10.-15.04.2011. m y a k o v , A. Bö r n e r , S. La n d j e v a , T. Ka r c e v a , E.K. Kh l e s t k i n a , A.V. V140. Na g e l , M.: Erhaltung von pflanzengenetischen Res- Si m o n o v , E.V. Mo r o z o v a & L.V. Sh c h u k i n a (vorgetragen von sourcen in der Bundeszentralen ex situ Genbank Pshenichnikova , T.A.): Prospects for the use of cytogenetic Gatersleben. – Interdisziplinäres Wissenschaftlertreffen collections and their derivative forms in bread wheat im Rahmen des Übereinkommens über die Biologi- for identification and mapping of new genes. – Inter- sche Vielfalt, Naturschutzakademie Vilm, Putbus, 22.- national Conference “Wheat Genetic Resources and Ge- 26.08.2011. nomics”, Novosibirsk/Russia, 28.08.-01.09.2011.

183 Vorträge und Poster/Lectures and Posters

V152. Pshenichnikova , T.A., E. Kh l e s t k i n a , L.V. Sh c h u k i n a , A.V. Si m o n - V165. Sc h r e i b e r , F.: Visualisation of biological data. – Otto-von- o v , A.K. Ch i s t y a k o v a , E.V. Mo r o z o v a , S. La n d j e v a , T. Ka r c e v a & Guericke-Universität Magdeburg, 11.02.2011. A. Bö r n e r (vorgetragen von Pshenichnikova , T.A.): Exploita- V166. Sc h r e i b e r , F.: Creating views on integrated multi-domain tion of Saratovskaya 29 / Janetzkis Probat 4D*7A substi- data and representing knowledge in SBGN. – Friedrich- tution and derivate lines for comprehensive phenotyp- Schiller-Universität, Jena, 16.06.2011. ing and molecular mapping of quantitative traits loci V167. Sc h r e i b e r , F.: Von Genen zu Netzen – Bioinformatik in Biolo- (QTL). – EWAC-EUCARPIA Cereals Section Conference, gie und Medizin. – Summerbyte, Halle/S., 11.08.2011. Novi Sad/Serbia, 07.-11.11.2011. V168. Sc h r e i b e r , F.: Current developments in pathway-related V153. Re h m a n Ar i f , M.A., M. Na g e l , U. Lo h w a s s e r & A. Bö r n e r Bioinformatics. – Novosibirsk State University, Russia, (vorgetragen von Re h m a n Ar i f , M.A.): Long-term seed 15.09.2011. storability in genebank collections – genetic studies V169. Sc h r e i b e r , F.: Creating views on integrated multi-domain in wheat. – Agrisafe Final Conference “Climate change: data. – Institutstag IPK, Gatersleben, 04.-06.10.2011. challenge for training of applied plant scientists“, Buda- V170. Sc h r e i b e r , F.: Bioinformatics @ IPK Gatersleben. – Instituts- pest/Hungary, 21.-23.03.2011. tag IPK, Gatersleben, 04.-06.10.2011. V154. Re h m a n Ar i f , M.A.: Seed conservation in ex situ genebanks V171. Sc h r e i b e r , F.: The Systems Biology Graphical Notation – – genetic studies on longevity in wheat. – 7th Plant methods and tools. – German-Russian Workshop, Bio- Science Student Conference, IPB, Halle/S., 14.-17.06.2011. technica Hannover, 10.10.2011. V155. Ro h n , H.: Network-based integrative visualization of bio- V172. Sc h r e i b e r , F.: Representing, analysing and visualising plant logical multi-domain data. – International German/Rus- metabolism. – Rheinische Friedrich-Wilhelms-Universität sian Summer School in “Integrative Biological Pathway Bonn, 22.11.2011. Analysis and Simulation”, Bielefeld, 04.-07.07.2011. V173. Sc h u b e r t , I.: Centromere maintenance. – State Key Labora- V156. Ro l l e t s c h e k , H.: Combined non-invasive imaging and tory of Plant Genomics, Centre for Genome Biology, In- modelling approaches reveal metabolic compartmen- stitute of Genetics and Developmental Biology, Chinese tation in the cereal endosperm. – 71st Harden Confer- Academy of Sciences, Beijing/China, 10.05.2011. ence on Metabolic Pathway Analysis, University of V174. Sc h u b e r t , I.: Mechanics of chromosome and karyotype Chester, Cheshire, England/UK, 19.-23.09.2011. evolution helps to interprete genomic data correctly. V157. Ru t t e n , T.: CENH3 dynamics in the plant cell cycle. – Fac- – National Maize Improvement Center, Chinese Agricul- ulty of Biology and Environment Protection, University tural University, Beijing/China, 10.05.2011. of Silesia, Katowice/Poland, 29.11.2011. V175. Sc h u b e r t , I.: Mechanics of chromosome and karyotype V158. Ša r h a n o v á , P., R.J. Va š u t , K.A. Ge e t h a , T.F. Sh a r b e l , M. Da n č á k , evolution helps to interprete genomic data correctly. – M. Ki t n e r & B. Tr á v n í č e k (vorgetragen von Ša r h a n o v á , P.): Wuhan University, Beijing/China, 13.05.2011. Microevolutionary processes in Central European Rubus V176. Sc h u b e r t , I.: Korrekte Interpretation der Genomevolu- populations: apomixis versus sexuality – stability versus tion erfordert Kenntnis der Chromosomenmutagenese. innovation. – EU COST Action Apomixis Workshop, Va- – Genetik und Epigenetik höherer Pflanzen, Wissen- lencia/Spain, 04.-05.07.2011. schaftliches Symposium anlässlich des 80. Geburtstages V159. Sc h a l l a u , A., F. Ar z e n t o n , A.J. Jo h n s t o n , U. Hä h n e l , D. Kö s z e g i , von Professor Rudolf Hagemann, Deutsche Akademie F.R. Bl a t t n e r , L. Al t s c h m i e d , T.F. Sh a r b e l , G. Ha b e r e r , G. Ba r - der Naturforscher Leopoldina, Halle/S., 28.10.2011. c a c c i a & H. Bä u m l e i n (vorgetragen von Sc h a l l a u , A.): Iden- V177. Sc h u b e r t , I.: Plant centromeres. – Japanese-German JSPS tification and genetic analysis of the apospory locus in and DFG-funded Workshop “Frontiers of Plant Chromo- Hypericum perforatum. – Plant and Animal Genome XIX. some Research: Centromeres and Artificial Chromo- Conference, San Diego/USA, 15.-19.01.2011. somes”, IPK Gatersleben, 31.10.-03.11.2011. V160. Sc h a l l a u , A.: The apospory locus of Hypericum perfora- V178. Sc h u b e r t , V.: Is there CENH3 loading during meiosis in tum. – 4th Annual Hypericum Meeting, Graz/Austria, 23.- plants? – Japanese-German JSPS and DFG-funded 24.10.2011. Workshop “Frontiers of Plant Chromosome Research: V161. Sc h m u t z e r , T.: RNA-seq in rye (Secale cereale L.): Establish- Centromeres and ArtificialC hromosomes”, IPK Gatersle- ment of new resources in Triticeae genomics. – 7th Plant ben, 31.10.-03.11.2011. Science Student Conference, IPB, Halle/S., 14.-17.06.2011. V179. Sc h w e i z e r , P.: HIGS: Host-induced gene silencing in phy- V162. Sc h m u t z e r , T.: Tackling repetitiveness in plant genomes: topathogenic fungi. – Plant and Animal Genome XIX. An introduction how to process NGS sequencing data Conference, San Diego/USA, 15.-19.01.2011. of plants. – International German/Russian Summer V180. Sc h w e i z e r , P.: Cell-wall related genes for race-nonspecific School in “Integrative Biological Pathway Analysis and resistance of barley to powdery mildew. – University of Simulation”, Bielefeld, 04.-07.07.2011. Adelaide, Adelaide/Australia, 23.09.2011. V163. Sc h n u r b u s c h , T.: Spike branching in wheat and barley. – V181. Sc h w e i z e r , P.: Race-nonspecific resistance of barley to 11th GABI Status Seminar, Potsdam, 15.-17.03.2011. powdery mildew: one trait – many genes. – SCIRO Plant V164. Sc h n u r b u s c h , T.: The rise of developmental mutants in Industry, Canberra/Australia, 26.09.2011. barley and wheat. – Seminar, Carlsberg Research Labo- V182. Se i f e r t , M.: Extended Hidden Markov Models for the ratory, Copenhagen/Denmark, 10.-11.11.2011. analysis of MeDIP-chip, tumor expression and Array-

184 CGH profiles. –I nstitute of Biology at the Ecole Normale V196. Ti k h e n k o , N., A. Bö r n e r , N. Ts v e t k o v a , T. Ru t t e n & A. Vo y l o k o v Supérieure, Paris/France, 18.02.2011. (vorgetragen von Ti k h e n k o , N.): Development of a model V183. Se i f e r t , M.: Exploiting prior knowledge and gene dis- system to study gene interactions of parental forms in tances in the analysis of tumor expression profiles with wheat-rye hybrids and primary Triticale. – International extended Hidden Markov Models. – Jstacs Workshop, Conference “Wheat Genetic Resources and Genomics”, Martin-Luther-Universität Halle-Wittenberg, Halle/S., Novosibirsk/Russia, 28.08.-01.09.2011. 19.05.2011. V197. Tr a u t w e i n , A., D. Ja n k o w s k a & G. Ku n z e (vorgetragen von V184. Se i f e r t , M.: Analyzing tumor expression and comparative Tr a u t w e i n A.): Purine degradation pathway in Arxula genomics data with extended Hidden Markov Mod- adeninivorans – basis for production of food with low els. – Interfakultäres Institut für Genetik und Funktio- purine content. – Institute of Microbial Technology (IM- nelle Genomforschung, Ernst-Moritz-Arndt-Universität, TECH). Chandigarh/India, 14.02.2011. Greifswald, 04.08.2011. V198. va n Ie r s e l , M.P. & T. Cz a u d e r n a (vorgetragen von v a n Ie r s e l , V185. Sreenivasulu , N.: Strategies for improved seed yield and M.P.): LibSBGN status update. – COMBINE 2011, Heidel- quality under terminal drought. – University of Agri- berg, 03.-07.09.2011. cultural Sciences, Crop Physiology Department, GKVK, V199. v o n Wi r é n , N.: Ammonium and iron sensing in Arabidop- Bangalore/India, 11.01.2011. sis plants. – Institut für Pflanzenbiochemie, Halle/S., V186. Sreenivasulu , N.: Reprogramming of seed metabo- 11.01.2011. lism under drought. – Kolloquien Pflanzenzüchtung, V200. v o n Wi r é n , N.: Ammonium and iron sensing in Arabidop- Martin-Luther-Universität Halle-Wittenberg, Halle/S., sis plants. – Universität Heidelberg, 19.01.2011. 10.06.2011. V201. v o n Wi r é n , N.: Ammonium and iron sensing in Arabidop- V187. Sreenivasulu , N.: Exploring yield enhancement genes sis plants. – Freie Universität Berlin, 01.07.2011. influencing grain number and seed growth under V202. v o n Wi r é n , N.: Chancen und Risiken der grünen Gentech- drought. – IZN Annual Meeting, Martin-Luther-Univer- nik. – Lion’s Club, Wernigerode, 19.07.2011. sität Halle-Wittenberg, Halle/S., 13.07.2011. V203. v o n Wi r é n , N.: Nutrient sensing in Arabidopsis. – Instituts- V188. Sreenivasulu , N.: Seed transcriptome reprogramming tag IPK, Gatersleben, 04.-06.10.2011. under terminal drought to harness optimum yield and V204. Vu, G.T.H. & I Sc h u b e r t (vorgetragen von Sc h u b e r t , I. seed. – PBGB Seminar, International Rice Research Ins- und Vu, G.T.H.): Genome sequencing in carnivorous titute, Metro Manila/Philippines, 14.09.2011. plants to study genome size and karyotype evolu- V189. Sreenivasulu , N.: Importance of ABA homeostasis for seed tion. – Julius-von-Sachs-Institut für Biowissenschaften, transcriptome reprogramming under terminal drought Lehrstuhl Botanik, Molekulare Pflanzenphysiologie to harness optimum yield and seed quality. – Instituts- und Biophysik, Julius-Maximilians-Universität Würz- tag IPK, Gatersleben, 04.-06.10.2011. burg, 21.10.2011. V190. St e i n , N.: New achievements in crop genomics – or how V205. We s c h k e , W.: Ertragssteigerungen bei Weizen – der to unlock the 5 gigabase barley genome. – Seminar, mögliche Beitrag der Grünen Gentechnik. – 25. Ge- Leibniz-Universität Hannover, 02.02.2011. treide-Tagung, Detmold, 17.03.2011. V191. St e i n , N.: Making the barley genome accessible – V206. We s c h k e , W.: Ertragssteigerungen bei Weizen – der mögli- progress in physical mapping and sequencing a 5 che Beitrag der Grünen Gentechnik. – Sitzung des Aus- Gbp genome. – Seminar, University of Bologna/Italy, schusses für Müllerei-Technologie, Würzburg, 22.03.2011. 23.02.2011. V207. We s c h k e , W.: Transgenic solutions to enhance yield struc- V192. St e i n , N.: Sequencing the 5.1 gigabase genome of bar- ture of high performance lines of wheat. – German- ley – new insights into Triticeae genome organization. – Brazilian Workshop on Transgenic Crops, Hannover, 04.- Seminar, Carlsberg Research Laboratory, Copenhagen/ 05.04.2011. Denmark, 04.03.2011. V208. We s c h k e , W.: Relationship between senescence-induced V193. St e i n , N.: Beauty and the beast – unlocking the barley N-remobilization and seed filling in barley. – Meeting genome by chromosomal genomics. – Botanikerta- DFG-Forschergruppe FOR948 „Nitrogen uptake, me- gung 2011 “Diversity makes the Difference”, Berlin, 18.- tabolism and remobilization in leaves during plant se- 23.09.2011. nescence”, Berlin, 23.-24.09.2011. V194. St e u e r n a g e l , B.: Challenges in assembling barley sequenc- V209. We s c h k e , W.: The role of cellular disintegration in crop es. – 3rd Workshop on TritiGen COST Action FA0604 “Triti- seed development. – Institutstag IPK, Gatersleben, 04.- ceae Genomics for The Advancement of Essential Euro- 06.10.2011. pean Crops”, Istanbul/Turkey, 03.-04.05.2011. V210. Wi t z e l , K., A. Ma t r o s , A. Mø l l e r, C. Fi n n i e , B. Sv e n s s o n , G. Ku n - V195. Te o , C.H.: Induction of telomere-mediated chromosom- z e & H. P. Mo c k (vorgetragen von Wi t z e l , K.): Label-free al truncation and stability of truncated chromosomes quantitative proteomics of the plasma membrane leads in Arabidopsis thaliana. – Japanese-German JSPS and to the identification of new candidates for increasing DFG-funded Workshop “Frontiers of Plant Chromosome salt tolerance in barley. – Meeting COST Action FA0603, Research: Centromeres and Artificial Chromosomes”, Dijon/France, 25.-27.05.2011. IPK Gatersleben, 31.10.-03.11.2011.

185 Vorträge und Poster/Lectures and Posters

V211. Za y n a l i Ne z h a d , K., W.E. We b e r , M.S. Rö d e r , S. Sh a r m a , U. Lo h - M. Mo r g a n t e , P. He d l e y , R. Wa u g h , A. Gr a n e r & N. St e i n : The w a s s e r , R.C. Me y e r , B. Sa a l & A Bö r n e r (vorgetragen von Za y - physical map of the barley genome. – Institutstag IPK, n a l i Ne z h a d , K.): QTL analysis for thousand-grain weight Gatersleben, 04.-05.10.2010. under terminal drought stress in bread wheat (Triticum P10. Ar i y a d a s a , R., D. Sc h u l t e , N. Poursarebani , R. Zh o u , B. St e u e r n a - aestivum L.). – International Conference “Wheat Genetic g e l , U. Sc h o l z , M. Ma r t i s , K. Ma y e r , H. Liu, P. He d l e y , R. Wa u g h , P. Resources and Genomics”, Novosibirsk/Russia, 28.08.- La n g r i d g e , T. Cl o s e , A. Gr a n e r & N. St e i n : The physical map of 01.09.2011. barley (Hordeum vulgare L.). – 10th Gatersleben Research Conference 2010 (GRCX) “Sequence-informed Crop Re- search”, Quedlinburg (Palais Salfeldt)/Gatersleben (IPK), 22.-24.11.2010. Poster/Posters P11. Ba k o s , F., C. Ka s t n e r , B. Ba r n a b á s , E. Ku g e l m a n n & J. Ku m l e h n : Generation of doubled haploid maize plants following inductive treatment of isolated immature pollen. – Con- 2010 ference on Green Plant Breeding Technology, Vienna/ Austria, 02.-05.02.2010. P1. Ab s a t t a r o v a , A.S., M.S. Rö d e r , S. Ko l l e r s , A.I. Mo r g o u n o v & S. P12. Ba r u n a w a t i , N.: Influence ofN itrogen Fertilizer application Ke n j e b a e v a : Identification and distribution of the alleles on iron retranslocation in wheat plants. – 15th Interna- of photoperiod and vernalization responses wheat tional Symposium on Iron Nutrition and Interactions in genes in Kazakhstan. – 8th International Wheat Confer- Plants, Budapest/Hungary, 26.-30.06.2010. ence, St. Petersburg/Russia, 01.-04.06.2010. P13. Be r g e r , C., G. He n s e l , M. Gu r u s h i d z e , D. Do u c h k o v , R. Ei c h m a n n , P2. Ag a r w a l , R., A. Ma t r o s , M. Me l z e r , H.-P. Mo c k & K. Sainis: I. Sc h ö n b o r n , L. Lo y a l l , S. Bi e r i , R. Hü c k e l h o v e n , P. Sc h w e i - Blue native PAGE analysis validates heterogeneity in z e r & J. Ku m l e h n : Generation and molecular analysis of the thylakoids of Synechocystis 6803. – 15th Interna- transgenic barley and wheat lines carrying hairpin RNAi tional Congress of Photosynthesis, Beijing/China, 22.- constructs directed towards genes involved in plant- 27.08.2010. pathogen interaction. – 10th GABI Status Seminar, Pots- P3. Ah k a m i , A.H., M. Me l z e r , B. Ha u s e , P. Fr a n k e n , U. Dr ü g e & dam, 09.-11.03.2010. M.R. Ha j i r e z a e i : Influence of auxin on primary metabo- P14. Bo h n e r , A.: Physiological functions of the urea transpor- lism at various developmental stages of root formation ter AtDUR3 in Arabidopsis thaliana. – 6th Plant Science in Petunia. – 20th IPGSA (International Plant Growth Student Conference, IPK, Gatersleben, 15.-18.06.2010. Substances Association)-Conference, Tarragona/Spain, P15. Bo h n e r , A.: Physiological functions of the urea transporter 28.06.-02.07.2010. AtDUR3 during senescence in Arabidopsis thaliana. – 5th P4. Al t e r , P., J.-S. An, B. St e u e r n a g e l , T. Sc h m u t z e r , U. Sc h o l z , T. European Plant Senescence Network Meeting (SENnet Dr e s s e l h a u s & M. Ga h r t z : Maize stem parenchyma – de- 2010), Wokefield Park, Reading/UK, 31.08.-03.09.2010. veloping tools for the engineering of a potential storage P16. Bo h n e r , A.: Physiological function of the urea transpor- tissue. – Maize Genetics Conference, Riva del Garda/Italy, ter AtDUR3 during senescence in Arabidopsis thaliana. 18.-21.03.2010. – Deutsche Gesellschaft für Pflanzenernährung (DGP) P5. Al t s c h m i e d , L.: Promoters of Arabidopsis thaliana are char- Symposium, Hannover, 30.09.-02.10.2010. acterized by local n-mer frequencies. – Institutstag IPK, P17. Bo r i s o v a , B., G. Jo v t c h e v , I. Sc h u b e r t & M.F. Me t t e : Genetic Gatersleben, 04.-05.10.2010. analysis of tandem repeat mediated control of nu- P6. An d e d e n , E., F. Ba l o c h , B. Kilian, N. Na c h i t & H. Öz k a n : Allelic clear architecture in A. thaliana. – Biowissenschaftli- diversity for vernalization and photoperiod genes in ches Netzwerk „Strukturen und Mechanismen der bi- bread wheat cultivars and landraces from Turkey. – 8th ologischen Informationsverarbeitung“, Gesamtarbeits International Wheat Conference, St. Petersburg/Russia, besprechung, Martin-Luther-Universität Halle-Witten- 01.-04.06.2010. berg, Halle/S., 04.-05.03.2010. P7. An g i o i , S.A., D. Ra u , E. Be l l u c c i , L. Na n n i , H. Kn ü p f f e r , V. Ne g r i , P18. Bo r i s o v a , B., I. Sc h u b e r t & J. Fu c h s : The sex chromatin of P.L. Sp a g n o l e t t i Ze u l , R. Pa p a & G. At t e n e : European runner Rumex acetosa reveals distinct histone methylation bean landraces: genetic diversity and structure assessed marks. – Biowissenschaftliches Netzwerk „Strukturen by chloroplast microsatellites. – 54th Annual Congress of und Mechanismen der biologischen Informationsver- the Italian Society of Agricultural Genetics (SIGA), Mat- arbeitung“, Gesamtarbeitsbesprechung, Martin-Luther- era/Italy, 27.-30.09.2010. Universität Halle-Wittenberg, Halle/S., 04.-05.03.2010. P8. Ap p e l h a g e n , I., G. Hu e p , T. Al t m a n n , B. We i s s h a a r & M. Sa g a s - P19. Boudichevskaia , A., S. Vo i g t l ä n d e r & R. Sc h m i d t : Analysis of s e r : Weird fingers – functional analysis of I W P domain regulators of seed oil biosynthesis. – Biowissenschaftli- proteins. – 21st International Conference on Arabidopsis ches Netzwerk „Strukturen und Mechanismen der Research, Yokohama/Japan, 06.-10.06.2010. biologischen Informationsverarbeitung“, Gesamtar- P9. Ar i y a d a s a , R., D. Sc h u l t e , N. Poursarebani , R. Zh o u , B. St e u e r - beitsbesprechung, Martin-Luther-Universität Halle- n a g e l , U. Sc h o l z , M. Ma r t i s , M. Se i d e l , H. Gu n d l a c h , K. Ma y e r , Wittenberg, Halle/S., 04.-05.03.2010. S. Ta u d i e n , M. Fe l d e r , M. Pl a t z e r , B. Sh i , P. La n g r i d g e , T. Cl o s e ,

186 P20. Boudichevskaia , A., J. Ko p p o l u & R. Sc h m i d t : Analysis of regu- P33. Da g h m a , D., K. Ho f f i e , T. Ru t t e n , M. Wi e s n e r & M. Me l z e r : High lators of seed oil biosynthesis. – 6th Plant Science Stu- pressure freezing of immature pollen using Cyanobac- dent Conference, IPK, Gatersleben, 15.-18.06.2010. teria as ‘filler’ for loading nitrocellulose capillaries. – 17th P21. Boudichevskaia , A., J. Ko p p o l u & R. Sc h m i d t : Genetic analysis International Microscopy Congress, Rio de Janeiro/Bra- of seed traits in Arabidopsis thaliana. – Institutstag IPK, zil, 19.-24.09.2010. Gatersleben, 04.-05.10.2010. P34. Da g h m a , D. & M. Me l z e r : Structural and ultrastructural P22. Br e u i n g , A., C. Za n k e , A. Se n u l a , E.R.J. Ke l l e r , H. Wa i b e l & T. study of barley pollen embryogenesis. – 12th Interna- Wi n k e l m a n n : Kostenanalyse der Langzeitlagerung von tional EMBL PhD Student Symposium, Heidelberg, 21.- Knoblauch in Genbanken. – Gemeinsame Tagung des 23.10.2010. Arbeitskreises Deutsche In Vitro Kulturen (ADIVK) und P35. Da g h m a , D.S., T. Ru t t e n , K. Ho f f i e , M. Wi e s n e r & M. Me l z e r : der Gesellschaft für Pflanzenbiotechnologie e.V. „Pflan- Cyanobacteria as biological filler for high pressure zenbiotechnologie in Deutschland – wo stehen wir?“, freezing of embryonic pollen. – 10th GABI Status Semi- Hannover, 13.-15.09.2010. nar, Potsdam, 09.-11.03.2010. P23. Br u c h m ü l l e r , A., M. Ku h l m a n n , J. Ku m l e h n , R. Sc h m i d t & M.F. P36. Da g h m a , D.S., T. Ru t t e n , G. He n s e l , J. Ku m l e h n & M. Me l z e r : Me t t e : RNA-directed RNA polymerase RDR5 is essential Live-cell imaging of immature barley pollen – A new ap- for transcript level-mediated post-transcriptional gene proach to study the initiation of pollen embryogenesis. – silencing. – 21st International Conference on Arabidopsis Institutstag IPK, Gatersleben, 04.-05.10.2010. Research, Yokohama/Japan, 06.-10.06.2010. P37. De h m e r , K.J. & L. Kr a w e t z k e : An SSR-based assessment of P24. Ca o , H.X., A. Boudichevskaia & R. Sc h m i d t : Exploiting BAC diversity in wild species of potato. – 2nd International libraries for the study of the Brassica napus genome. – Symposium on Genomics of Plant Genetic Resources, Institutstag IPK, Gatersleben, 04.-05.10.2010. Bologna/Italy, 24.-27.04.2010. P25. Ch e s n o k o v , Y.V., N.V. Po c h e p n y a , L. Ko z l e n k o , O.P. Mi t r o f a n o - P38. De h m e r , K.J.: Assessing genetic diversity in potato wild v a , U. Lo h w a s s e r & A. Bö r n e r : Localization of QTLs for ag- species via SSR markers. – EAPR – EUCARPIA Congress ronomic important characters in spring wheat (Triticum on Potato Breeding after Completion of the DNA Se- aestivum L.) grown in different ecological places in Rus- quence of the Potato Genome, Wageningen/The Ne- sia and Germany. – 8th International Wheat Conference, therlands, 27.-30.06.2010. St. Petersburg/Russia, 01.-04.06.2010. P39. De h m e r , K.J.: Wild potatoes and fluorescent SSRs: diver- P26. Co l m s e e , C., S. So n n e w a l d , U. Sc h l ü t e r , A. Br ä u t i g a m , N. sity assessment in potato genetic resources. – 7th So- Ze l l e r h o f f , A. We b e r , M. Bu c h e r , U. So n n e w a l d & U. Sc h o l z : lanaceae Conference (SOL2010), Dundee, Scotland/UK, OPTIMAS-OC: Development of an oligo chip to explore 05.-09.09.2010. the maize transcriptome. – 10th GABI Status Seminar, P40. De m i d o v , D., I. Le r m o n t o v a , T. Ru t t e n , K. Ri e h a , J. Fu c h s & A. Potsdam, 09.-11.03.2010. Ho u b e n : Characterisation of Arabidopsis Aurora kinases P27. Co l m s e e , C., M. Klapperstück , M. La n g e , S. Fl e m m i n g & U. Sc h o l z : and other spindle checkpoint proteins. – Chroma- Efficient management of high-throughput primary lab tin2010 – Transcription, chromatin structure and DNA data. – 6th International Symposium on Integrative Bioin- repair in development and differentiation, Zeche Zoll- formatics 2010, Cambridge/UK, 22.-24.03.2010. verein, Essen, 07.-10.07.2010. P28. Cz a u d e r n a , T., A. Ju n k e r , C. Kl u k a s & F. Sc h r e i b e r : SBGN-ED P41. De m i d o v , D., O. We i s s , S. He s s e , T. Ru t t e n , J. Fu c h s & A. Ho u - for editing, translating and validating SBGN maps and b e n : Characterisation of Aurora-like kinases in plants. – RIMAS as a first application. – 6th International Sympo- International Conference “Molecular Aspects of Plant sium on Integrative Bioinformatics 2010, Cambridge/ Development”, Vienna/Austria, 23.-26.02.2010. UK, 22.-24.03.2010. P42. Dö l l , S. & H.-P. Mo c k : Identification and functional char- P29. Cz a u d e r n a , T., A. Ju n k e r , C. Kl u k a s , A. Ha r t m a n n , H. Bä u m l e i n acterisation of genes involved in the biosynthesis of the & F. Sc h r e i b e r : SBGN-ED for editing, translating and vali- coumarin scopolin. – Institutstag IPK, Gatersleben, 04.- dating SBGN maps and RIMAS as a first application. – 05.10.2010. Institutstag IPK, Gatersleben, 04.-05.10.2010. P43. Do u c h k o v , D., S. Lü c k & P. Sc h w e i z e r : A high-throughput P30. Cz a u d e r n a , T., C. Kl u k a s , A. Ju n k e r & F. Sc h r e i b e r : Editing, val- RNAi screen for defense- or susceptibility-related genes idating, and translating of SBGN maps with SBGN-ED. – in barley (GABI-Phenome). – 10th GABI Status Seminar, 11th International Conference on Systems Biology (ICSB Potsdam, 09.-11.03.2010. 2010), Edinburgh, Scotland/UK, 10.-15.10.2010. P44. Do u c h k o v , D., S. Lü c k & P. Sc h w e i z e r : High-throughput RNAi P31. Cz e r n i c k a , M., E. Bö e r , B. St e u e r n a g e l , S. Fl e m m i n g , N. St e i n , U. of multigene families for quantitative resistance in bar- Sc h o l z & G. Ku n z e : The Arxula adeninivorans genome: Se- ley. – Institutstag IPK, Gatersleben, 04.-05.10.2010. quencing, assembly and annotation. – Institutstag IPK, P45. Do u c h k o v , D., S. Lü c k & P. Sc h w e i z e r : High-throughput RNAi Gatersleben, 04.-05.10.2010. of multigene families for quantitative resistance in bar- P32. Da g h m a , D.S., J. Ku m l e h n & M. Me l z e r : Structural markers ley. – 10th Gatersleben Research Conference 2010 (GRCX) of Pollen Embryogenesis (POEM). – 10th GABI Status “Sequence-informed Crop Research”, Quedlinburg (Pa- Seminar, Potsdam, 09.-11.03.2010. lais Salfeldt)/Gatersleben (IPK), 22.-24.11.2010.

187 Vorträge und Poster/Lectures and Posters

P46. Er n s t , M., R.C. Me y e r & T. Al t m a n n : GABI-ENERGY: Biomass P58. Fr e i s t , S., I. Ot t o , G. He n s e l , K. Boutilier, T.F. Sh a r b e l & J. production in maize – genomics guided breeding of Ku m l e h n : Genetic transformation of Boechera polyantha energy maize and a systems-oriented analysis; geno- (formerly B. holboellii) – the way forward to function- typing of inbred line panel and phenotyping of inbreds ally characterize genes involved in apomictic repro- and test crosses under glasshouse conditions. – 10th duction. – Gemeinsame Tagung des Arbeitskreises GABI Status Seminar, Potsdam, 09.-11.03.2010. Deutsche In Vitro Kulturen (ADIVK) und der Gesellschaft P47. Fe d o s e y e n k o , D., C. We i s h a a r & N. Vo n Wi r é n : Investigating für Pflanzenbiotechnologie e.V. „Pflanzenbiotechnolo- beneficial plant microbe interaction using metabolomic gie in Deutschland – wo stehen wir?“, Hannover, 13.- approach. – Deutsche Gesellschaft für Pflanzenernährung 15.09.2010. (DGP) Symposium, Hannover, 30.09.-02.10.2010. P59. Fr i e d e l , S., J. Ke i l w a g e n & M. Se i f e r t : Large-scale transcrip- P48. Fi n k e , A., M. Ku h l m a n n & M.F. Me t t e : Genetic and molecular tome analysis of different types of the abiotic stress in analysis of RNA-directed transcriptional gene silencing Arabidopsis thaliana. – Institutstag IPK, Gatersleben, in Arabidopsis. – 6th Meeting of the GBM Study Section 04.-05.10.2010. RNA Biochemistry, Hohenwart, 30.09.-03.10.2010. P60. Fu c h s , J., B. Bo r i s o v a , G. Jo v t c h e v & I. Sc h u b e r t : Epigenetic P49. Fi n k e , A., M. Ku h l m a n n & M.F. Me t t e : Genetic and molecular modifications of sex chromatin in dioecious plants. –I n- analysis of RNA-directed transcriptional gene silencing stitutstag IPK, Gatersleben, 04.-05.10.2010. in Arabidopsis. – 6th Plant Science Student Conference, P61. Ga l l a , G., T.F. Sh a r b e l & G. Ba r c a c c i a : Apospory in Hyperi- IPK, Gatersleben, 15.-18.06.2010. cum perforatum L.: from cytohistological investigations P50. Fi n k e , A., M. Ku h l m a n n & M.F. Me t t e : Genetic analysis of of sporogenesis and gametogenesis to transcriptome RNA-directed transcriptional gene silencing in Ara- analysis. – 14th International Biotechnology Symposium bidopsis thaliana. – Institutstag IPK, Gatersleben, 04.- and Exhibition, Rimini/Italy, 14.-18.09.2010. 05.10.2010. P62. Ga l l a , G., T.F. Sh a r b e l & G. Ba r c a c c i a : Apospory in Hyperi- P51. Fl a v e l l , A., X. Xu, J. Co m a d r a n , W. Th o m a s , D. Ma r s h a l l , J. Ru s - cum perforatum L.: from cytohistological investigations s e l l , R. Wa u g h , A. To n d e l l i , L. Ca t t i v e l l i , F. Schnaithmann , K. of sporogenesis and gametogenesis to transcriptome Pi l l e n , A. Sc h u l m a n , C. In g v a r d s e n , S. Ra s m u s s e n , R. Sh a r m a & analysis. – 54th Annual Congress of the Italian Soci- B. Kilian: An incremental approach for association map- ety of Agricultural Genetics (SIGA), Matera/Italy, 27.- ping of useful traits in barley – The EXBARDIV Project. 30.09.2010. – 2nd International Symposium on Genomics of Plant P63. Ga w r o n s k i , P. & T. Sc h n u r b u s c h : A few gene deletion causes Genetic Resources, Bologna/Italy 24.-27.04.2010. early flowering in an einkorn wheat mutant (Triticum P52. Fo r n e r , S., K. Mi e l k e , U. Co n r a d & B. Ha u s e : Tissue and organ monococcum L.). – 6th Plant Science Student Confer- specificity of jasmonate response in development of to- ence, IPK, Gatersleben, 15.-18.06.2010. mato (Solanum lycopersicum) flowers. – th6 Plant Science P64. Ga w r o n s k i , P., N. St e i n & T. Sc h n u r b u s c h : A few gene dele- Student Conference, IPK, Gatersleben, 15.-18.06.2010. tion causes early flowering in an einkorn wheat mutant P53. Fr a n c i a , E., M. Pasquariello , D. Ba r a b a s c h i , A. To n d e l l i , D. (Triticum monococcum L.). – Institutstag IPK, Gatersle- Sc h u l t e , N. St e i n , E.J. St o c k i n g e r , M.A. St a n c a & N. Pe c c h i o n i : ben, 04.-05.10.2010. Towards physical mapping and sequencing the Fr-H2 P65. Ge r n a n d -Kl i e f o t h , D., J. Fu c h s , F. Ei c k m e y e r & K.J. De h m e r : (Frost Resistance-H2) region of barley chromosome 5H. Application of the GISH technique and PCR markers – Plant and Animal Genome XVIII. Conference, San Die- for more efficient breeding programs in the grass gen- go/USA, 09.-13.01.2010. era Dactylis, Festulolium and Phleum. – Institutstag IPK, P54. Fr a n k e , M., C. Kr a c h , H. Po s k a r , N. Sc h ä f e r , L. Fi c h t m ü l l e r, F. Gatersleben, 04.-05.10.2010. Ke l l n e r & B.H. Ju n k e r : Steady-state 13C metabolic flux anal- P66. Gh o l a m i , M., D.K. Ze l t n e r , R. Sc h m i d t , W. Ec k e & R. Sn o w d o n : ysis of developing crop seeds. – JSMC/JCB Workshop Multiplexed 454 amplicon sequencing for high-through- “Microbes, Models, and Methods”, Jena, 09.-10.03.2010. put SNP discovery in Brassica napus candidate genes for P55. Fr a n k e , M., C. Kr a c h , H. Po s k a r & B.H. Ju n k e r : Determina- oil content. – Brassica 2010, 17th Crucifer Genetics Work- tion of metabolic fluxes in developing crop seeds using shop, Saskatoon/Canada, 05.-09.09.2010. steady-state 13C metabolic flux analysis. – METABOLOM- P67. Gi e h l , R.F.H., J.E. Li m a & N. v o n Wi r é n : Local supply of iron ICS, Amsterdam/The Netherlands, 27.06.-01.07.2010. distinctly defines lateral root number and elongation P56. Fr a n k e , M., C. Kr a c h , H. Po s k a r & B.H. Ju n k e r : 13C metabolic in Arabidopsis thaliana. – XVII Conference of the Fed- flux analysis on developing crop plants. – 11th Interna- eration of European Societies of Plant Biology (FESPB tional Conference on Systems Biology (ICSB 2010), Edin- 2010), Valencia/Spain, 04.-09.07.2010. burgh, Scotland/UK, 10.-15.10.2010. P68. Gi e h l , R., H. Ta k a h a s h i & N. v o n Wi r é n : Nutriomics-based P57. Fr e i s t , S., I. Ot t o , G. He n s e l , K. Boutilier, T.F. Sh a r b e l & J. identification of transcription factors involved in nutri- Ku m l e h n : Genetic transformation of Boechera polyantha ent accumulation and efficiency inArabidopsis thaliana. (formerly B. holboellii) – the way forward to functionally – Deutsche Gesellschaft für Pflanzenernährung (DGP) characterize genes involved in apomictic reproduction. Symposium, Hannover, 30.09.-02.10.2010. – XXI International Congress on Sexual Plant Reproduc- tion, Bristol/UK, 02.-06.08.2010.

188 P69. Gi e h l , R.F.H., J.E. Li m a & N. v o n Wi r é n : Local supply of iron P81. Ha i l e , J., K. Ha m m e r , M.M. Na c h i t , A. Ba d e b o & M.S. Rö d e r : distinctly defines lateral root number and elongation QTL analysis in an intraspecific recombinant inbred in Arabidopsis thaliana. – Institutstag IPK, Gatersleben, population of durum wheat (Triticum turgidum L. var. 04.-05.10.2010. durum) to recent Eastern African isolate of stem rust, P70. Gi e r s b e r g , M., T. Ge r l a c h , M. Ka i s e r , P.T. Mi n h Ha, A. Ni e t e r , Puccinia graminis f.sp. tritici, Ug99. – Borlaug Global Rust S. Uh l i g & G. Ku n z e : Environmental pollution control by Initiative 2010, Technical Workshop, St. Petersburg/Rus- yeast based biosensors. – Institutstag IPK, Gatersleben, sia, 30.-31.05.2010. 04.-05.10.2010. P82. Ha i l e , J., K. Ha m m e r , A. Ba d e b o , M.M. Na c h i t & M.S. Rö d e r : P71. Go e d e k e , S., G. He n s e l , C. Ka y d a m o v , S. Br o e d e r s , I. Sa a l b a c h & QTL Analysis for resistance to stem rust including re- J. Ku m l e h n : Production of recombinant proteins in trans- cently available Ethiopian races of Ug99 in durum genic barley grains. – Meeting on Molecular Farming: wheat. – 10th Gatersleben Research Conference 2010 Plants as a Production Platform for High Value Proteins, (GRCX) “Sequence-informed Crop Research”, Quedlin- Vico Equense/Italy, 06.-09.10.2010. burg (Palais Salfeldt)/Gatersleben (IPK), 23.-24.11.2010. P72. Go h r , A., J. Gr a u , J. Ke i l w a g e n , R. Eg g e l i n g , B. Ha l d e m a n n , M. P83. Ha j i r e z a e i , M.-R.: Improvement of biomass production: Mo h r , M. Sc h a r f e , M. Se i f e r t , I. Gr o s s e & S. Po s c h : JSTACS – A resources, opportunities and constraints. – 16th Inter- Java framework for statistical analysis and classification national Conference on Renewable Resources and of biological sequences. – 27th International Conference Plant Biotechnology, NAROSSA®2010, Magdeburg, 07.- on Machine Learning (ICML 2010), Haifa/Israel, 21.- 08.06.2010. 24.06.2010. P84. Ha r p k e , D., H. Ke r n d o r f f , T. Ru t t e n & F.R. Bl a t t n e r : Phylog- P73. Go l e m i e c , E., D. Dä n h a r d t , G. He n s e l , J. Ku m l e h n , U. Vo i g t & eny of Crocus based on rDNA ITS sequences. – Instituts- K. Kr u p i n s k a : Barley Whirly1-RNAi plants show delayed tag IPK, Gatersleben, 04.-05.10.2010. senescence at high light intensity. – 5th European Plant P85. Harshavardhan , V.T., C. Se i l e r , K. Ra j e s h , H. Ro l l e t s c h e k , Senescence Network Meeting (SENnet 2010), Wokefield G. He n s e l , J. Ku m l e h n , U. Wo b u s & N. Sreenivasulu : A trans- Park, Reading/UK, 31.08.-03.09.2010. genic strategy for improved seed filling under drought P74. Gr u b e r , B., R. Gi e h l & N. v o n Wi r é n : Transciptional regula- stress by altering the ABA metabolism in barley. – 10th tion of manganese homeostasis in Arabidopsis thaliana. GABI Status Seminar, Potsdam, 09.-11.03.2010. – Deutsche Gesellschaft für Pflanzenernährung (DGP) P86. Ha r t m a n n , A., A. Ju n k e r , E. Gr a f a h r e n d -Be l a u , M. Kl a p p e r - Symposium, Hannover, 30.09.-02.10.2010. s t ü c k , K. Hi p p e , C. Co l m s e e , S. We i s e , U. Sc h o l z , B.H. Ju n k e r & P75. Gr y c z k a , C., D. Kő s z e g i , G. He n s e l , D. Da g h m a , J. Ku m l e h n & F. Sc h r e i b e r : MetaCrop: an information system for crop H. Bä u m l e i n : Pollen embryogenesis related gene expres- plant metabolism. – Institutstag IPK, Gatersleben, 04.- sion. – International Conference “Molecular Aspects of 05.10.2010. Plant Development”, Vienna/Austria, 23.-26.02.2010. P87. Ha s e n e y e r , G., T. Sc h m u t z e r , M. Se i d e l , C.-C. Sc h ö n , K. Ma y e r , P76. Gr y c z k a , C., D. Kö s z e g i , G. He n s e l , D. Da g h m a , J. Ku m l e h n & U. Sc h o l z , N. St e i n & E. Ba u e r : GABI RYE-EXPRESS: Using H. Bä u m l e i n : Molecular analysis of pollen embryogen- next-generation technologies for improvement of ge- esis in barley. – 10th GABI Status Seminar, Potsdam, 09.- netic and genomic resources in rye. – 10th GABI Status 11.03.2010. Seminar, Potsdam, 09.-11.03.2010. P77. Gu r u s h i d z e , M., E. Ku g e l m a n n , S. Go t t w a l d , N. St e i n & J. Ku m - P88. Ha s e n e y e r , G., T. Sc h m u t z e r , M. Se i d e l , K. Ma y e r , C.-C. Sc h o e n , l e h n : Generation of instantly homozygous barley mu- U. Sc h o l z , N. St e i n & E. Ba u e r : A functional genomics re- tants from EMS-treated embryogenic pollen cultures. source for the EXPRESSed portion of the rye genome. – Conference on Green Plant Breeding Technology, Vi- – 10. GPZ Haupttagung 2010 „Innovations in Breeding enna/Austria, 02.-05.02.2010. Methodology”, Technische Universität München, Frei- P78. Gu r u s h i d z e , M., E. Ku g e l m a n n , S. Go t t w a l d , N. St e i n & J. Ku m - sing-Weihenstephan, 15.-17.03.2010. l e h n : Generation of instantly homozygous barley mutants P89. Ha s e n e y e r , G., T. Sc h m u t z e r , M. Se i d e l , C.-C. Sc h o e n , R. Zh o u , from EMS-treated embryogenic pollen cultures. – 10th K. Ma y e r , U. Sc h o l z , N. St e i n & E. Ba u e r : New genomics re- GABI Status Seminar, Potsdam, 09.-11.03.2010. sources for rye (Secale cereale L.). – Conference on Ge- P79. Gu s e , T., E. Gr ü t z e m a n n , J. Tu b i s , C. Ma r t h e & J. Ku m l e h n : nomics-Based Breeding of the GPZ Ag Genome Analy- Transformation von Weizen durch Agrobakterien-ver- sis, Justus-Liebig-Universität, Gießen, 26.-28.10.2010. mittelten Gentransfer in in vitro kultivierten Samen- P90. Ha u p t m a n n , V., F. Ju n g h a n s , K. Sc h a l l a u , M. Me n z e l , P. Gu n k e l , anlagen. – Gemeinsame Tagung des Arbeitskreises U. Sp o h n & U. Co n r a d : Plant-based expression of recom- Deutsche In Vitro Kulturen (ADIVK) und der Gesellschaft binant spider silk protein derivates and their mechani- für Pflanzenbiotechnologie e.V. „Pflanzenbiotechnolo- cal characterisation. – Bio Meets Nano and IT, Halle/S., gie in Deutschland – wo stehen wir?“, Hannover, 13.- 24.11.2010. 15.09.2010. P91. He c k m a n n , S., K. Na g a k i & A. Ho u b e n : Inverted Meiosis – in P80. Gu s e , T., C. Ma r t h e , E. Gr ü t z e m a n n , J. Tu b i e s & J. Ku m l e h n : Ge- the holocentric chromosome species Luzula elegans. – netic transformation of wheat via injection of Agrobac- DFG Priority Programme SPP1384 – Mechanisms of Ge- terium into isolated ovules. – Institutstag IPK, Gatersle- nome Haploidization/Annual Meeting, EMBL, Heidel- ben, 04.-05.10.2010. berg, 22.-24.03.2010.

189 Vorträge und Poster/Lectures and Posters

P92. He c k m a n n , S., K. Na g a k i & A. Ho u b e n : Inverted Meiosis – in P103. Ho a n g , P.T., T.B. Le, D. Fl o s s , J. Sc h e l l e r & U. Co n r a d : Overex- the holocentric chromosome species Luzula elegans. – pression and purification of the elastin-like polypeptide 6th Plant Science Student Conference, IPK, Gatersleben, fusion influenza H5N1 antigens in Nicotiana tabacum. 15.-18.06.2010. – COST Meeting “Molecular Farming”, Napoli/Italy, 06.- P93. He c k m a n n , S., I. Le r m o n t o v a , A. Ho u b e n , H. Bä u m l e i n & I. Sc h u - 08.10.2010. b e r t : Regulation of CENH3 expression in Arabidopsis P104. Ho u s t o n , K., A. Dr u k a , N. Bo n a r , J. Fr a n c k o w i a k , U. Lu n d q v i s t , thaliana. – Chromatin2010 – Transcription, chromatin J. Al e x a n d e r , M. Mo r g a n t e , N. St e i n , D. Ha r r a p & R. Wa u g h : structure and DNA repair in development and differen- Fine mapping and identification of the candidate genes tiation, Zeche Zollverein, Essen, 07.-10.07.2010. for barley third outer glume (trd) locus. – Plant and P94. He d t m a n n , C., A. Ma t r o s , G. Ku n z e , G. d e Ja e g e r & H.-P. M o c k : Animal Genome XVIII. Conference, San Diego/USA, 09.- Mass spectrometry analysis to elucidate the interac- 13.01.2010. tion partners of plant co-chaperones with homology P105. Ja n i a k , A., S. Pi o r k o , B. Ch m i e l e w s k a , A. Ma t r o s , H.-P. Mo c k to the human protein HOP (heat shock organizing & I. Sz a r e j k o : A comparative analysis of protein expres- protein). – 43. Jahrestagung der Deutschen Gesell- sion during root hair initiation in barley. – International schaft für Massenspektrometrie (DGMS), Halle/S., 07.- Conference “Molecular Aspects of Plant Development”, 10.03.2010. Vienna/Austria, 23.-26.02.2010. P95. He d t m a n n , C., S. Ka s p a r , R. Li p p m a n n , S. Dö l l , A. Ma t r o s , P106. Janmohammadi , M., A. Ma t r o s , R.T. Af s h a r i , S. Ma h f o o z i , H. G. d e Ja e g e r , K. Wi t z e l & H.-P. Mo c k : Analysis of abiotic Al i z a d e h & H.-P. Mo c k : Proteome analysis of freeze toler- stress responses by proteomic approaches. – XVII Con- ant winter wheat during transition from the vegetative ference of the Federation of European Societies of Plant to the reproductive phase under field condition. – 3rd Biology (FESPB 2010), Valencia/Spain, 04.-09.07.2010. Iranian Proteomics Congress, Pasteur Institute, Tehran/ P96. He d t m a n n , C., S. En d r e s s , A. Ma t r o s , G. d e Ja e g e r & H.-P. M o c k : Iran, 26.-27.05.2010. Biochemical analysis of plant co-chaperone function P107. Jo h n s t o n , A.J. & W. Gr u i s s e m : Dosage-sensitive function of and their potential interaction partners. – Institutstag RETINOBLASTOMA RELATED and convergent epigenetic IPK, Gatersleben, 04.-05.10.2010. control are required during the Arabidopsis life cycle. – P97. He n s e l , G., D. Da g h m a , D. Kő s z e g i , U. Zi e r o l d , H. Bä u m l e i n , 21st International Conference on Arabidopsis Research, M. Me l z e r & J. Ku m l e h n : Unravelling initial mechanisms of Yokohama/Japan, 06.-10.06.2010. pollen embryogenesis: Microspore-specific transgene P108. Jo s t , M., F. Sh a h i n n i a , A. Dr u k a , R. Wa u g h , M. Mo r g a n t e & expression and visualisation of sub-cellular structures N. St e i n : Molecular mapping of the gene Laxatum (lax.a) in fixed and viable pollen. – 10th GABI Status Seminar, on barley chromosome 5H. – 6th Plant Science Student Potsdam, 09.-11.03.2010. Conference, IPK, Gatersleben, 15.-18.06.2010. P98. He n s e l , G., D. No w a r a , G. Zi m m e r m a n n , A. Ga y , J. Ku m l e h n & P109. Ka r i m i As h t i y a n i , R., T. Ru t t e n & A. Ho u b e n : AtHaspin, a P. S c h w e i z e r : Resistenzerhöhung bei Gerste und Weizen histone H3-specific kinase in Arabidopsis thaliana. – mittels pflanzlicher Expression von RNAi-Konstrukten Forschungscluster-C (FC-C/Epigenetic), Halle/S., 04.- mit konservierten Sequenzen pathogener Pilze. – Ge- 05.03.2010. meinsame Tagung des Arbeitskreises Deutsche In Vitro P110. Ka r i m i As h t i y a n i , R., T. Ru t t e n & A. Ho u b e n : Arabidopsis Kulturen (ADIVK) und der Gesellschaft für Pflanzenbio- Haspin, a histone H3-specific kinase with potential role technologie e.V. „Pflanzenbiotechnologie in Deutschland in meristem development? – SEB (Society for Experi- – wo stehen wir?“, Hannover, 13.-15.09.2010. mental Biology) Annual Main Meeting, Prague/Czech P99. He n s e l , G., H. Bä u m l e i n , H.-P. Mo c k , M. Me l z e r , L. Al t s c h m i e d , Republic, 30.06.-03.07.2010. D.S. Da g h m a , C. Gr y c z k a , D. Ko s z e g i , R. Li p p m a n n , A. Ma t r o s , P111. Ka r i m i As h t i y a n i , R., D. De m i d o v , T. Ru t t e n & A. Ho u b e n : Func- K. Pl a s u n & J. Ku m l e h n : GABI-POEM – a project aiming to tional characterisation of histone kinases. – Institutstag elucidate initial mechanisms of pollen embryogenesis. IPK, Gatersleben, 04.-05.10.2010. – Institutstag IPK, Gatersleben, 04.-05.10.2010. P112. Ka s p a r , S., A. Ma t r o s , W. We s c h k e & H.-P. Mo c k : LC-MS P100. Hi m m e l b a c h , A. & P. Sc h w e i z e r : Complementation for en- based protein analysis of microdissected tissues re- gineering nonhost resistance in wheat against wheat vealed cell-type specific biological functions in devel- powdery mildew using barley BAC clones (GABI- oping barley grains. – 43. Jahrestagung der Deutschen Phenome). – 10th GABI Status Seminar, Potsdam, 09.- Gesellschaft für Massenspektrometrie (DGMS), Halle/S., 11.03.2010. 07.-10.03.2010. P101. Ho a n g , P.T., T.B. Le & U. Co n r a d : Expression and purifica- P113. Ka s p a r , S., K. Wi t z e l , B. Sc h u l z , H. Me h l h o r n , F. Sc h r e i b e r & tion of recombinant antigens of the H5N1 avian flu H.-P. Mo c k : BetaNet: Biochemical network analysis for virus in Nicotiana tabacum. – 6th Plant Science Student the identification of novel candidates associated with Conference, IPK, Gatersleben, 15.-18.06.2010. increased sucrose levels in sugar beet. – 10th GABI Sta- P102. Ho a n g , P.T., T.B. Le, D. Fl o s s , J. Sc h e l l e r & U. Co n r a d : Purifica- tus Seminar, Potsdam, 09.-11.03.2010. tion of elastin-like polypeptide fusion H5N1 influenza P114. Ka s t n e r , C., A. Hi m m e l b a c h , G. Dö h l e m a n n , P. Sc h w e i z e r , antigens by membrane based technology. – Instituts- G. He n s e l , M. Ga h r t z & J. Ku m l e h n : Etablierung eines tag IPK, Gatersleben, 04.-05.10.2010. Agrobakterien-vermittelten Transformationssystems und

190 dessen Anwendung zur Untersuchung des Pathogen-in- ish chromosome. – 10th Gatersleben Research Confer- duzierbaren HvGER4c Promotors in Mais. – Gemeinsame ence 2010 (GRCX) “Sequence-informed Crop Research”, Tagung des Arbeitskreises Deutsche In Vitro Kulturen Quedlinburg (Palais Salfeldt)/Gatersleben (IPK), 22.- (ADIVK) und der Gesellschaft für Pflanzenbiotechnologie 24.11.2010. e.V. „Pflanzenbiotechnologie in Deutschland – wo stehen P126. Kn ü p f f e r , H. & M. Op p e r m a n n : The expeditions of Hans wir?“, Hannover, 13.-15.09.2010. Stubbe in Greece and Albania in 1941-1942, and the P115. Ke m p e , K., M. Ru b t s o v a & M. Gi l s : A novel hybrid seed sys- present status of the material collected in the IPK Gene- tem for wheat. – 10th GABI Status Seminar, Potsdam, 09.- bank. – Institutstag IPK, Gatersleben, 04.-05.10.2010. 11.03.2010. P127. Ko b i l j s k i , B., A. Bö r n e r , A. Ko n d i c -Sp i k a , S. De n c i c , D. Tr k u l j a P116. Kh l e s t k i n a , E.K., O.Y. Tereshchenko , T.A. Pshenichnikova , V.S. & L. Br b a k l i c : How to validate potentially useful quan- Ar b u z o v a , M.S. Rö d e r , A. Bö r n e r & E.A. Sa l i n a : Flavonoid titative trait loci for efficient implementation of mark- biosynthesis genes in wheat: genome location and er-assisted selection in wheat breeding. – 8th Interna- function. – 8th International Wheat Conference, St. Pe- tional Wheat Conference, St. Petersburg/Russia, 01.- tersburg/Russia, 01.-04.06.2010. 04.06.2010. P117. Kim, Y.-M., J.V. Kö b e r , I. Ja k o b , J.O. Gi e s e , M. Me l z e r , U. So n - P128. Ko h l , S., J. Ho l l m a n n , V. Ra d c h u k , M. Mü l l e r, B. St e u e r n a g e l , n e w a l d , N. v o n Wi r é n & M.-R. Ha j i r e z a e i : Silencing of to- T. Sc h m u t z e r , U. Sc h o l z , H. We b e r & W. We s c h k e : New 454-se- bacco HXK1a isoform strongly affects plant growth. – quence information reflecting the relationship between 6th Plant Science Student Conference, IPK, Gatersleben, N-remobilization and grain filling in barley. – 10th Gaters- 15.-18.06.2010. leben Research Conference 2010 (GRCX) “Sequence- P118. Kim, Y.-M., J.V. Kö b e r , I. Ja k o b , J.O. Gi e s e , M. Me l z e r , U. So n - informed Crop Research”, Quedlinburg (Palais Salfeldt)/ n e w a l d , N. v o n Wi r é n & M. Ha j i r e z a e i : Silencing of Tobacco Gatersleben (IPK), 22.-24.11.2010. HXK1a isoform strongly affects plant growth. – Institut- P129. Ko l l e r s , S., M.S. Rö d e r , V. Ko r z u n , E. Eb m e y e r , O. Ar g i l l i e r, stag IPK, Gatersleben, 04.-05.10.2010. P. Jo a q u i m , D. Ku l o s a , B. Ro d e m a n n & M. Ga n a l : Population P119. Kim, Y.-M., J.V. Kö b e r , I. Ja k o b , J.O. Gi e s e , M. Me l z e r , U. So n - structure in a collection of elite West-European germ- n e w a l d , N. v o n Wi r é n & M. Ha j i r e z a e i : Silencing of To- plasm of hexaploid wheat (Triticum aestivum L.). – 10th bacco HXK1a isoform strongly affects plant growth. GABI Status Seminar, Potsdam, 09.-11.03.2010. – 4th International Symposium of the SFB 429 “Signals, P130. Ko l l e r s , S., M.S. Rö d e r , V. Ko r z u n , E. Eb m e y e r , O. Ar g i l l i e r, Sensing and Plant Primary Metabolism”, Potsdam, 06.- P. Jo a q u i m , D. Ku l o s a , B. Ro d e m a n n & M. Ga n a l : Population 09.10.2010. structure in a collection of elite West-European germ- P120. Kirioukhova, O., C. Ka e g i , A.J. Jo h n s t o n , H. Bä u m l e i n , R. Gr o s s - plasm of hexaploid wheat (Triticum aestivum L.). – 10. Ha r d t & U. Grossniklaus : A novel Arabidopsis orthologue GPZ Haupttagung 2010 „Innovations in Breeding Meth- of Inner Centromere Protein is essential for reproduc- odology”, Technische Universität München, Freising- tive development. – XXI International Congress on Sex- Weihenstephan, 15.-17.03.2010. ual Plant Reproduction, Bristol/UK, 02.-06.08.2010. P131. Ko l l e r s , S., H. Lu e r s s e n , R. Cz i h a l , M.W. Ga n a l & M.S. Rö d e r : P121. Kl e g e r , A., D. Ma l a n , B. Fl e i s c h m a n n , M. Wa r t e n b e r g , M. Ze n c k e , SNP genotyping in hexaploid wheat (Triticum aestivum G. Ad l e r , A.M. Wo b u s & S. Li e b a u : Modulation of calcium L.) with Illumina BeadXpress. – Institutstag IPK, Gater- activated potassium channels induces cardiogenesis of sleben, 04.-05.10.2010. pluripotent stem cells and enrichment of pacemaker- P132. Ko l l e r s , S., H. Lu e r s s e n , R. Cz i h a l , M.W. Ga n a l & M.S. Rö d e r : like cells. – 3rd International Congress on Stem Cells and SNP genotyping in hexaploid wheat (Triticum aestivum Tissue Formation, Dresden, 11.-14.07.2010. L.) with Illumina BeadXpress. – 10th Gatersleben Re- P122. Kl e m m e , S., J. Do l e z e l , K. Ma y e r , N. St e i n , T. Wi c k e r , J. Fu c h s search Conference 2010 (GRCX) “Sequence-informed & A. Ho u b e n : Sequence characterization of rye B-chro- Crop Research”, Quedlinburg (Palais Salfeldt)/Gatersle- mosomes. – 6th Plant Science Student Conference, IPK, ben (IPK), 22.-24.11.2010. Gatersleben, 15.-18.06.2010. P133. Ko m a t s u d a , T., N. Wa n g , S. Na i r , Y. Tu r u s p e k o v , M. Po u r k h e i - P123. Kl e m m e , S., M. Ma r t i s , A.M. Ba n a e i Mo g h a d d a m , K. Ma y e r , r a n d i s h , S. Sinsuwongwat , M. Sa m e r i , G. Ch e n , H. Ka n a m o r i , T. Wi c k e r , J. Ma c a s , U. Sc h o l z , F. Bu l l , T. Sc h m u t z e r , H. Sim- I. Ho n d a , Y. Wa t a n a b e , N. St e i n , T. Wi c k e r , A. Ta g i r i , Y. Na g a - k o v a , J. Do l e z e l , N. St e i n , J. Fu c h s & A. Ho u b e n : Evolution m u r a & T. Ma t s u m o t o : Biological function of barley cleis- and drive mechanism of a selfish chromosome. – Insti- togamy gene cly1. – Plant and Animal Genome XVIII. tutstag IPK, Gatersleben, 04.-05.10.2010. Conference, San Diego/USA, 09.-13.01.2010. P124. Kl u k a s , C., I. Mü c k e , H. Me h l h o r n & F. Sc h r e i b e r : Develop- P134. Ko n o v a l o v , F., B. Kilian & A. Ku d r y a v t s e v : Inferring evolu- ment of the Integrated Analysis Platform (IAP) an infor- tionary relationships in wild and cultivated wheat spe- mation system for automated plant phenotyping. – In- cies based on polymorphic retrotransposon insertions. stitutstag IPK, Gatersleben, 04.-05.10.2010. – International Conference “Plant Genetics, Genomics, P125. Kl e m m e , S., M. Ma r t i s , A.M. Ba n a e i Mo g h a d d a m , H. Gu n d l a c h , and Biotechnology 2010” (PlantGen), Novosibirsk/Rus- K. Ma y e r , T. Wi c k e r , J. Ma c a s , P. No v a k , P. Ne u m a n n , U. Sc h o l z , sia, 07.-10.06.2010. F. Bu l l , T. Sc h m u t z e r , H. Si m k o v a , J. Do l e z e l , N. St e i n , J. Fu c h s & A. Ho u b e n : Evolution and drive mechanism of a self-

191 Vorträge und Poster/Lectures and Posters

P135. Ko n o v a l o v , F., S. Sh a a f , H. Öz k a n , A. Gr a n e r & B. Kilian: Mea- S. Ge n t z , E. Gr ü t z e m a n n , P. Ho f f m e i s t e r , A. Kn o s p e , S. Kn ü p f f e r , suring ‘the rest of the iceberg’: genetic diversity of wild S. Lü c k , C. Ma r t h e , A. Mü l l e r, I. Ot t o , S. Wo l f & G. He n s e l : wheat assessed by retrotransposon-based markers. The cereal transformation platform at the IPK Gater- – 10th Gatersleben Research Conference 2010 (GRCX) sleben. – 10th Gatersleben Research Conference 2010 “Sequence-informed Crop Research”, Quedlinburg (Pa- (GRCX) “Sequence-informed Crop Research”, Quedlin- lais Salfeldt)/Gatersleben (IPK), 22.-24.11.2010. burg (Palais Salfeldt)/Gatersleben (IPK), 22.-24.11.2010. P136. Ko o h i De h k o u r d i , M., D. Ri e w e , R.C. Me y e r & T. Al t m a n n : Char- P147. Ku n z e , M., S. Kr a u s e , J. Ga s t a l d e l l o , G. Mö n k e & U. Co n r a d : acterization of candidate enzymes within metabolite Recombinant antibodies against ABA receptors: screen- QTL in Arabidopsis thaliana. – 6th Plant Science Student ing and characterization. – Institutstag IPK, Gatersleben, Conference, IPK, Gatersleben, 15.-18.06.2010. 04.-05.10.2010. P137. Ko o h i De h k o r d i , M., D. Ri e w e , M. Kh o d a m b a s h i , R.C. Me y e r & T. P148. La n d j e v a , S., U. Lo h w a s s e r & A. Bö r n e r : Mapping QTL for Al t m a n n : Characterization of candidate enzymes within germination, seed vigour and longevity on wheat D ge- metabolite QTL in Arabidopsis thaliana. – 10th Gatersle- nome. – Arbeitstagung der Arbeitsgemeinschaft Saat- ben Research Conference 2010 (GRCX) “Sequence-in- gut- und Sortenwesen der GPZ und GPW, IPK, Gatersle- formed Crop Research”, Quedlinburg (Palais Salfeldt)/ ben, 24.-25.02.2010. Gatersleben (IPK), 22.-24.11.2010. P149. La n g e , M., C. Co l m s e e , S. Fl e m m i n g , J. Ch e n , M. Klapperstück P138. Ko p p o l u , R., V. Va d e z , L. Krishnamurthy , S. Ni g a m , S. Is o b e , S. & U. Sc h o l z : The eDAL suite: tools and concepts for pri- Kn a p p , T. Ja y a k u m a r , G. He, D. Be r t i o l i , D. Ho i s i n g t o n , M. Bu t - mary data citation. – Institutstag IPK, Gatersleben, 04.- t e r f i e l d & R. Va r s h n e y : QTL analysis for drought tolerance 05.10.2010. in groundnut indicates involvement of several small-ef- P150. La p i n , D., R.C. Me y e r & G. v a n d e n Ac k e r v e k e n : Genetic map- fect main and epistatic QTL. – 6th Plant Science Student ping of broad resistance to downy mildew in Arabidop- Conference, IPK, Gatersleben, 15.-18.06.2010. sis thaliana C24. – Oomycete Molecular Genetics Net- P139. Ko p p o l u , R., T. Ko m a t s u d a & T. Sc h n u r b u s c h : Genetic control work Meeting, Toulouse/France, 06.-08.06.2010. of lateral spikelet fertility in barley – the role of the vrs4 P151. Le Ho n g , D., A.J. Jo h n s t o n , T. Ru t t e n & H. Bä u m l e i n : Repro- locus. – Institutstag IPK, Gatersleben, 04.-05.10.2010. ductive functions of EFFECTOR OF TRANSCRIPTION P140. Ko p p o l u , R., T. Ko m a t s u d a & T. Sc h n u r b u s c h : Isolation and (ET). – Institutstag IPK, Gatersleben, 04.-05.10.2010. characterization of candidates for the six-rowed spike4 P152. Le r m o n t o v a , I., S. He c k m a n n , I. Mo r a e s , T. Ru t t e n , J. Fu c h s (vrs4) gene in barley. – Conference on Genomics-Based & I. Sc h u b e r t : Functional characterisation of the plant Breeding of the GPZ Ag Genome Analysis, Justus-Lie- centromeric histone H3 variant and developmental big-Universität, Gießen, 26.-28.10.2010. consequences of its depletion. – EMBO Workshop on P141. Ko s z e g i , D., A.J. Jo h n s t o n , M. Mi l d n e r , S.E. Wu e s t , T. Ru t t e n , O. Chromosome Segregation and Aneuploidy, Edinburgh, Kirioukhova, U. Grossniklaus & H. Bä u m l e i n : Plant RKD tran- Scotland/UK, 19.-23.06.2010. scription factors reprogram sporophytic cells towards P153. Liiving, T. & B.H. Ju n k e r : Investigating the metabolism and an egg cell fate. – XXI International Congress on Sexual metabolic fluxes in legume embryos during seed filling Plant Reproduction, Bristol/UK, 02.-06.08.2010. phase with non-aqueous fractionation. – Conference on P142. Ko s z e g i , D., A.J. Jo h n s t o n , M. Mi l d n e r , S.E. Wu e s t , T. Ru t t e n , Regulation of Plant Growth, Potsdam, 12.-14.04.2010. O. Kirioukhova, U. Grossniklaus & H. Bä u m l e i n : Plant RKD P154. Liiving, T. & B.H. Ju n k e r : Investigating the metabolism and transcription factors reprogram sporophytic cells to- metabolic fluxes in legume embryos during seed fill- wards an egg cell fate. – Institutstag IPK, Gatersleben, ing phase with non-aqueous fractionation. – 6th Plant 04.-05.10.2010. Science Student Conference, IPK, Gatersleben, 15.- P143. Kr a c h , C., M. Fr a n k e , L. Fi c h t m ü l l e r, F. Ke l l n e r & B.H. Ju n - 18.06.2010. k e r : Towards metabolic flux maps of developing barley P155. Li p p m a n n , R., A. Ma t r o s , J. Ku m l e h n & H.-P. Mo c k : Mass spec- seeds. – Conference on Regulation of Plant Growth, trometric identification of potential feeder substances Potsdam, 12.-14.04.2010. beneficial to pollen embryogenesis. – 43. Jahrestagung P144. Kr e m e r , C., M. C, A. Se n u l a , E.R.J. Ke l l e r & M.E. Go n z á l e z - der Deutschen Gesellschaft für Massenspektrometrie Be n i t o : Cryopreservation of mint in vitro-apices: effect of (DGMS), Halle/S., 07.-10.03.2010. preculture and cooling procedures. – 47th Annual Meet- P156. Li p p m a n n , R., A. Ma t r o s , J. Ku m l e h n & H.-P. Mo c k : Mass spec- ing of the Society for Cryobiology (CRYO-2010), Bristol/ trometric identification of potential feeder substances UK, 17.-20.07.2010. beneficial to pollen embryogenesis. – 10th GABI Status P145. Ku m a r , U., A.K. Jo s h i , S. Ku m a r , R. Ch a n d & M.S. Rö d e r : Quan- Seminar, Potsdam, 09.-11.03.2010. titative trait loci for resistance to spot blotch caused by P157. Lo h w a s s e r , U., A. Di t t b r e n n e r , H.-P. Mo c k & A. Bö r n e r : Mo- Bipolaris sorokiniana in wheat (T. aestivum L.) lines ‘Ning lecular and biochemical studies of opium poppy (Pa- 8201’ and ‘Chirya 3’. – 8th International Wheat Confer- paver somniferum L.) – a basis for enhancing the quality ence, St. Petersburg/Russia, 01.-04.06.2010. of food and pharmaceutical aspects. – 2nd International P146. Ku m l e h n , J., C. Be r g e r , D. Do u c h k o v , T. Gu s e , A. Hi m m e l b a c h , Symposium on Genomics of Plant Genetic Resources, B. Me l z e r , E. Ka p u s i , C. Ka s t n e r , J. Ri e c h e n , P. Sc h w e i z e r , Bologna/Italy, 24.-27.04.2010. U. Zi e r o l d , G. Zi m m e r m a n n , C. Bo l l m a n n , H. Bü c h n e r , S. Fr e i s t ,

192 P158. Ma, L., C.H. Te o , E. Ka p u s i , G.T.H. Vu, G. He n s e l , J. Ku m l e h n , P169. Me y e r , R.C., F. Ar a n a -Ce b a l l o s , M. Er n s t , M. Ko o h i , M.M. Mu- M.F. Me t t e , I. Sc h u b e r t & A. Ho u b e n : Characterisation of bar- r a y a , D. Ri e w e , M. Ro s s o , M. Se y f a r t h , K. We i g e l t & T. Al t m a n n : ley chromosomes by fluorescent in situ hybridization. Genetic variation and heterosis of vegetative biomass – SEB (Society for Experimental Biology) Annual Main accumulation and metabolite composition in Arabidop- Meeting, Prague/Czech Republic, 30.06.-03.07.2010. sis thaliana and Zea mays. – Institutstag IPK, Gatersle- P159. Ma, L., G.T.H. Vu, V. Sc h u b e r t , K. Wa t a n a b e , N. St e i n , A. Ho u b e n ben, 04.-05.10.2010. & I. Sc h u b e r t : Synteny between Brachypodium distachyon P170. Me y e r , R.C., D. Ri e w e , K. We i g e l t , B. Eb e r t , R. Sc h m i d t , M. Er n s t , and Hordeum vulgare as revealed by FISH. – 10th Gaters- J. Li s e c & T. Al t m a n n : Uncovering the molecular basis of leben Research Conference 2010 (GRCX) “Sequence- heterosis for biomass in the model species Arabidopsis informed Crop Research”, Quedlinburg (Palais Salfeldt)/ thaliana. – Plant and Animal Genome XVIII. Conference, Gatersleben (IPK), 22.-24.11.2010. San Diego/USA, 09.-13.01.2010. P160. Ma r c o n i , G., G. Ga l l a , J. Co n n e r , L. Ra g g i , T.F. Sh a r b e l , P171. Me y e r , R.C., D. Ri e w e , K. We i g e l t , B. Eb e r t , R. Sc h m i d t , J. Li s e c , P. Oz i a l s -Ak i n s , G. Ba r c a c c i a , M. Fa l c i n e l l i & E. Al b e r t i n i : M. St e i n f a t h & T. Al t m a n n : Uncovering the molecular Cross-species characterization of APOSTART for unveil- basis of heterosis for biomass. – 7th Tri-National Arabi- ing its role in apomixis: could APOSTART be the gene dopsis Meeting (TNAM 2010), Salzburg/Austria, 15.- for the “apomictic revolution”? – 54th Annual Congress 18.09.2010. of the Italian Society of Agricultural Genetics (SIGA), P172. Mi n h Ha, P.T., M. Gi e r s b e r g , M. Kö r n e r , C. Ka i s e r , S. Uh l i g , Matera/Italy, 27.-30.09.2010. K. Ku n a t h , K. Fl o r s c h ü t z & G. Ku n z e : Immobilized Arxula P161. Ma r q u e s , A., M. Gu e r r a & A. Ho u b e n : Characterization of adeninivorans yeast cells as microbial compound of eu- and heterochromatin of Citrus by histone modifica- electrochemical biosensors for detection of estrogenic tion and DNA methylation analysis. – 6th Plant Science activities in waste water. – 6th Plant Science Student Student Conference, IPK, Gatersleben, 15.-18.06.2010. Conference, IPK, Gatersleben, 15.-18.06.2010. P162. Ma t t h i e s , I.E., S. We i s e , U. Sc h o l z & M.S. Rö d e r : GABI-GENO- P173. Mo r a e s , I., I. Le r m o n t o v a & I. Sc h u b e r t : How is CENH3 tar- BAR: Association mapping in barley – a genome wide geted to centromeric DNA? – EMBO Conference on and candidate gene approach. – 10th GABI Status Semi- Replication/Repair and Segregation of Chromosomes, nar, Potsdam, 09.-11.03.2010. Freiburg-Munzingen, 13.-17.07.2010. P163. Ma t t h i e s , I., S. We i s e & M.S. Rö d e r : Association mapping in P174. Mü l l e r, M., H. Sc h ö n , A. Sc h l e r e t h , W.-R. Sc h e i b l e , H. Wi t u c k a - barley – a genome wide and candidate gene approach. Wa l l , R.C. Me y e r , M. v o n Ko r f f , O. Th i m m , M. St i t t & T. Al t - – 10. GPZ Haupttagung 2010 „Innovations in Breeding m a n n : FUNCIN: Functional genomics to identify lead Methodology”, Technische Universität München, Frei- genes and metabolic markers to improve nitrogen sing-Weihenstephan, 15.-17.03.2010. utilization in crops WP2-7: Using natural diversity of P164. Ma u , M., J.M. Co r r a l , M. Me l z e r , J. Fu c h s , H. Vo g e l , D. Ge e l e n Arabidopsis. – 10th GABI Status Seminar, Potsdam, 09.- & T.F. Sh a r b e l : Unreduced pollen formation in the genus 11.03.2010. Boechera: Analysis of anther development and stage- P175. Na g e l , M., K. Ne u m a n n , U. Lo h w a s s e r , K. Fö r s t e r & A. Bö r n e r : specific global gene expression. – Institutstag IPK, Assoziationsgenetische Pilotstudie zur Langlebigkeit Gatersleben, 04.-05.10.2010. von Gerste (Hordeum vulgare L.). – Arbeitstagung der P165. Me l z e r , B., C. Be r g e r , T. Gu s e , E. Ka p u s i , G. He n s e l & J. Ku m - Arbeitsgemeinschaft Saatgut- und Sortenwesen der l e h n : Agrobacterium-mediated transformation of Bra- GPZ und GPW, IPK, Gatersleben, 24.-25.02.2010. chypodium distachyon using shoot base segments. – 6th P176. Na g e l , M., M.A. Re h m a n Ar i f , I. Kr a n n e r , A. Fi e d l e r , H. Sc h u l z Plant Science Student Conference, IPK, Gatersleben, & A. Bö r n e r : Physiologische und genetische Aspekte zur 15.-18.06.2010. Qualität von langzeitgelagertem Weizensaatgut. – 45. P166. Me l z e r , B., C. Be r g e r , T. Gu s e , E. Ka p u s i , G. He n s e l , C. Ma r t h e Vortragstagung der Deutschen Gesellschaft für Quali- & J. Ku m l e h n : Agrobacterium-mediated transformation of tätsforschung (DGQ), Berlin, 22.-23.03.2010. Brachypodium distachyon using shoot base segments. – P177. Na g e l , M., M.A. Re h m a n Ar i f , E. Wi l l n e r & A. Bö r n e r : Seed Gemeinsame Tagung des Arbeitskreises Deutsche In Vi- longevity – intra- and interspecific differences in the tro Kulturen (ADIVK) und der Gesellschaft für Pflanzenbio- Gatersleben genebank collections. – 29th Congress of technologie e.V. „Pflanzenbiotechnologie in Deutschland the International Seed Testing Association (ISTA), Köln – wo stehen wir?“, Hannover, 13.-15.09.2010. 2010, 15.-18.06.2010. P167. Me l z e r , B., C. Be r g e r , T. Gu s e , E. Ka p u s i , G. He n s e l , C. Ma r t h e P178. Na g e l , M., M.A. Re h m a n Ar i f , U. Lo h w a s s e r , K. Ne u m a n n , & J. Ku m l e h n : Agrobacterium-mediated transformation of H. Ts c h i e r s c h , H. Ro l l e t s c h e k , L. Bo r i s j u k , I. Kr a n n e r & Brachypodium distachyon using shoot base segments. – A. Bö r n e r : Seed deterioration: genetic and biochemical Institutstag IPK, Gatersleben, 04.-05.10.2010. impacts. – Institutstag IPK, Gatersleben, 04.-05.10.2010. P168. Me r x , K., R. Su p i l c e , W. We s c h k e , M. St i t t & H.-P. Mo c k : High P179. Na g e l , M., M. Ro s e n h a u e r , E. Wi l l n e r, F.-G. Sc h r ö d e r , R. Sn o w - throughput determination of enzyme activities and d o n , W. Fr i e d t & A. Bö r n e r : Seed longevity in canola (Bras- kinetic parameters in barley grains. – 6th Plant Science sica napus L.) – genetic variation and QTL mapping. – 2nd Student Conference, IPK, Gatersleben, 15.-18.06.2010. International Symposium on Genomics of Plant Genetic Resources, Bologna/Italy, 24.-27.04.2010.

193 Vorträge und Poster/Lectures and Posters

P180. Na g e l , M., M. Ro s e n h a u e r , E. Wi l l n e r, R. Sn o w d o w n , W. Fr i e d t mapping in natural populations. – 10th Gatersleben Re- & A. Bö r n e r : Genetic variation and background of seed search Conference 2010 (GRCX) “Sequence-informed longevity in canola (Brassica napus L.). – 6th Plant Science Crop Research”, Quedlinburg (Palais Salfeldt)/Gatersle- Student Conference, IPK, Gatersleben, 15.-18.06.2010. ben (IPK), 22.-24.11.2010. P181. Na v a k o d e , S., M. Na c h i t , M. Ba u m , M.S. Rö d e r & A. Bö r n e r : P193. Pe u k e r t , M., S. Ka s p a r , A. Ma t r o s , W. We s c h k e & H.-P. Mo c k : A genetic linkage map of durum wheat segregating MALDI-imaging and LC-based mass spectrometry for for drought, heat and salt tolerance. – 2nd International spatial protein and metabolite profiling during barley Symposium on Genomics of Plant Genetic Resources, grain development. – Institutstag IPK, Gatersleben, 04.- Bologna/Italy, 24.-27.04.2010. 05.10.2010. P182. Ne u m a n n , K., M. Ba u m , U. Lo h w a s s e r & A. Bö r n e r : Genome P194. Pe u k e r t , M., A. Ma t r o s & H.-P. Mo c k : MALDI-imaging: A wide association mapping – a strategy to unlock germ- tool for direct analysis of intact tissue sections. – 6th plasm collections. – Institutstag IPK, Gatersleben, 04.- Plant Science Student Conference, IPK, Gatersleben, 05.10.2010. 15.-18.06.2010. P183. Nü r k , N.M., S. Ma t r i ñ á n , M. Ca r i n e & F.R. Bl a t t n e r : Phylog- P195. Pistrick, K., M. Ak h a l k a t s i , M. Girgvliani & T. Shanshiashvili : eny and historical biogeography of Hypericum. – Botany Sammlung von Saat- und Pflanzgut pflanzengenetisch- 2010, Annual Meeting of the American Botanical Socie- er Ressourcen in Svanetien und Lečchumi (Geor- ties, Providence, Rhode Island/USA, 31.07.-04.08.2010. gien, Kaukasus) 2009. – Arbeitstagung der Arbeits- P184. Nü r k , N.M., S. Ma t r i ñ á n , M. Ca r i n e & F.R. Bl a t t n e r : Phyloge- gemeinschaft Saatgut- und Sortenwesen der GPZ und netic analysis and historical biogeography of Hypericum. GPW, IPK, Gatersleben, 24.-25.02.2010. – Institutstag IPK, Gatersleben, 04.-05.10.2010. P196. Pistrick, K., M. Ak h a l k a t s i , M. Girgvliani & T. Sh a n s h i a s h - P185. Op p e r m a n n , M., J. Ke i l w a g e n , H. Kn ü p f f e r , S. Fr i e d e l & v i l i : Collecting plant genetic resources in Svanetia and A. Bö r n e r : Validation, analysis and aggregation of long- Lečchumi (Georgia, Caucasus). – Institutstag IPK, Gaters- term trait observation data of genebank material. – An- leben, 04.-05.10.2010. nual Conference of the Taxonomic Databases Working P197. Pl a s u n , K., M. Mü l l e r, M. Me l z e r , T. Al t m a n n & J. Ku m l e h n : Group (TDWG 2010), Marine Biological Laboratory Arabidopsis plantlets obtained via anther culture origi- Woods Hole/USA, 26.09.-01.10.2010. nate from somatic cells. – 10th GABI Status Seminar, P186. Os i p o v a , S.V., A.V. Pe r m y a k o v , M.D. Pe r m y a k o v a , M.V. Ko v a l e n - Potsdam, 09.-11.03.2010. k o v a , T.A. Pshenichnikova & A. Bö r n e r : Leaf dehydroascor- P198. Pl a s u n , K., M. Mü l l e r, M. Me l z e r , T. Al t m a n n & J. Ku m l e h n : bate reductase and catalase activity is associated with Arabidopsis plantlets obtained via anther culture origi- drought tolerance in bread wheat. – International Con- nate from somatic cells. – XXI International Congress on ference “Plant Genetics, Genomics, and Biotechnology Sexual Plant Reproduction, Bristol/UK, 02.-06.08.2010. 2010” (PlantGen), Novosibirsk/Russia, 07.-10.06.2010. P199. Pl a s u n , K., M. Mü l l e r, M. Me l z e r , T. Al t m a n n & J. Ku m l e h n : P187. Pa l i w a l , R., A.K. Jo s h i , U. Ku m a r , J.P. Sr i v a s t a v a & M.S. Rö d e r : Arabidopsis plantlets obtained via anther culture origi- Genetic dissection of terminal heat tolerance in hexaploid nate from somatic cells. – Gemeinsame Tagung des wheat (T. aestivum L.). – Institutstag IPK, Gatersleben, 04.- Arbeitskreises Deutsche In Vitro Kulturen (ADIVK) und 05.10.2010. der Gesellschaft für Pflanzenbiotechnologie e.V. „Pflan- P188. Pa l i w a l , R., A.K. Jo s h i , U. Ku m a r , J.P. Sr i v a s t a v a & M.S. Rö d e r : zenbiotechnologie in Deutschland – wo stehen wir?“, Genetic dissection of terminal heat tolerance in hexaploid Hannover, 13.-15.09.2010. wheat (T. aestivum L.). – 10th Gatersleben Research Confer- P200. Po s k a r , H., C. Kr a c h , M. Fr a n k e , J. Hü g e , L. Fi c h t m ü l l e r, F. ence 2010 (GRCX) “Sequence-informed Crop Research”, Ke l l n e r , N. Sc h ä f e r & B. Ju n k e r : Flux maps for central me- Quedlinburg (Palais Salfeldt)/Gatersleben (IPK), 23.- tabolism of legume and cereal seeds. – Institutstag IPK, 24.11.2010. Gatersleben, 04.-05.10.2010. P189. Pa s a m , R., R. Sh a r m a , B. Kilian & A. Gr a n e r : Whole genome P201. Pu e n t e Mo l i n s , M., J.M. Co r r a l , O.M. Al i y u , J.L. Ma r o n & T.F. association mapping in spring barley. – 10th GABI Status Sh a r b e l : Hybridization and its role in apomictic repro- Seminar, Potsdam, 09.-11.03.2010. duction of St. John‘s wort (Hypericum perforatum L.). – P190. Pa s a m , R.K., R. Sh a r m a , G. Ha s e n e y e r , B. Kilian & A. Gr a n e r : Institutstag IPK, Gatersleben, 04.-05.10.2010. Whole genome association mapping in spring barley P202. Ra d c h u k , R., R.J.N. Em e r y , D. We i e r , H. Vi g e o l a s , P. Geigenberger , cultivars. – 10. GPZ Haupttagung 2010 „Innovations J.E. Lu n n , R. Fe i l , W. We s c h k e & H. We b e r : Sucrose non-fer- in Breeding Methodology”, Technische Universität menting kinase 1 (SnRK1) coordinates metabolic and hor- München, Freising-Weihenstephan, 15.-17.03.2010. monal signals during pea cotyledon growth and differen- P191. Pa s a m , R., R. Sh a r m a , G. Ha s e n e y e r , B. Kilian & A. Gr a n e r : QTL tiation. – International Conference “Molecular Aspects of identification for grain quality traits in barley using LD Plant Development”, Vienna/Austria, 23.-26.02.2010. mapping in natural populations. – Conference on Ge- P203. Ra d c h u k , V., G. He n s e l , N. We i c h e r t , J. Ku m l e h n & W. We s c h - nomics-Based Breeding of the GPZ Ag Genome Analysis, k e : Down-regulation of ABA 8’-hydroxylase 2 affects Justus-Liebig-Universität, Gießen, 26.-28.10.2010. spike number, seed set, seed development and germi- P192. Pa s a m , R., R. Sh a r m a , G. Ha s e n e y e r , B. Kilian & A. Gr a n e r : QTL nation in barley. – 10th GABI Status Seminar, Potsdam, identification for grain quality traits in barley using LD 09.-11.03.2010.

194 P204. Ra d c h u k , V., E. Po t o k i n a , D. We i e r , N. St e i n & W. We s c h k e : In- and climate change: scaling from mitochondria to the traspecies diversity of Jekyll genes influences grain yield globe”, Oxford/UK, 11.-14.04.2010 in barley. – 10th Gatersleben Research Conference 2010 P216. Ro s e n h a u e r , M., M. Na g e l , E. Wi l l n e r, F.-G. Sc h r ö d e r , R. Sn o w - (GRCX) “Sequence-informed Crop Research”, Quedlin- d o w n , W. Fr i e d t & A. Bö r n e r : Genetische Untersuchungen burg (Palais Salfeldt)/Gatersleben (IPK), 22.-24.11.2010. des Merkmals Langlebigkeit von Saatgut der Spezies P205. Ra d c h u k , V., R. Ra d c h u k , J. Ku m l e h n , N. Sreenivasulu , T. Ru t - Brassica napus L. – Arbeitstagung der Arbeitsgemein- t e n & L. Bo r i s j u k : Jekyll is crucial for tapetal degradation schaft Saatgut- und Sortenwesen der GPZ und GPW, and pollen development and pollen release in barley. IPK, Gatersleben, 24.-25.02.2010. – International Conference “Molecular Aspects of Plant P217. Ro s s o , M.G., M. Go t t o w i k , R. Lo s s e , G. We h r s t e d t & T. Al t m a n n : Development”, Vienna/Austria, 23.-26.02.2010. GABI-TILL – expansion and novel use of the GABI-Tilling P206. Ra j a r a m a n , J., D. Do u c h k o v , R. Hü c k e l h o v e n & P. Sc h w e i z e r : platform for gene function analysis in Arabidopsis. – 21st A partially duplictated gene is involved in quantitative International Conference on Arabidopsis Research, Yo- resistance of barley. – Institutstag IPK, Gatersleben, kohama/Japan, 06.-10.06.2010. 04.-05.10.2010. P218. Ru b t s o v a , M., K. Ke m p e & M. Gi l s : Development of an inno- P207. Ra j e s h , K., V.T. Harshavardhan , H. Ro l l e t s c h e k , K.P. Gö t z , vative hybrid seed production system for wheat. – Insti- C. Se i l e r , M. St r i c k e r t , V. Ko r z u n , B. Us a d e l , R. Su l p i c e , M. St i t t , tutstag IPK, Gatersleben, 04.-05.10.2010. U. Wo b u s & N. Sreenivasulu : Importance of senescence in- P219. Sa l i n a , E.A., I.N. Le o n o v a , E.M. Eg o r o v a , E.B. Bu d a s h k i n a & duced remobilization in determining the crop yield in M.S. Rö d e r : Development and application of Triticum elite lines of barley under terminal drought. – 10th GABI timopheevii mapping data in wheat resistance manage- Status Seminar, Potsdam, 09.-11.03.2010. ment strategies. – 8th International Wheat Conference, P208. Ri e c h e n , J., A. Va r s h n e y , G. He n s e l , P. Sc h w e i z e r & J. Ku m l e h n : St. Petersburg/Russia, 01.-04.06.2010. Etablierung einer Mehltau-Resistenz bei Weizen durch P220. Sa n e i , M., R. Pickering, S. Na s u d a & A. Ho u b e n : Analysis of Epidermis-spezifischeA bregulation von MLO. – Gemein- uniparental elimination of chromosome in wide cross- same Tagung des Arbeitskreises Deutsche In Vitro Kul- es. – SEB (Society for Experimental Biology) Annual Main turen (ADIVK) und der Gesellschaft für Pflanzenbiotech- Meeting, Prague/Czech Republic, 30.06.-03.07.2010. nologie e.V. „Pflanzenbiotechnologie in Deutschland – P221. Sa n e i , M., R. Pickering, S. Na s u d a & A. Ho u b e n : Analysis of wo stehen wir?“, Hannover, 13.-15.09.2010. uniparental elimination of chromosome in wide cross- P209. Ri e w e , D., M. Ko o h i De h k o u r d i , J. Li s e c , R.C. Me y e r , L. Wi l l m i t z e r es. – 10th Gatersleben Research Conference 2010 (GRCX) & T. Al t m a n n : Identification of metabolic TQ L candidate “Sequence-informed Crop Research”, Quedlinburg (Pa- genes in Arabidopsis. – 21st International Conference on lais Salfeldt)/Gatersleben (IPK), 22.-24.11.2010. Arabidopsis Research, Yokohama/Japan, 06.-10.06.2010. P222. Sc h a l l a u , A., F. Ar z e n t o n , A.J. Jo h n s t o n , U. Hä h n e l , D. Ko s z e g i , P210. Ro d e , J., P. Kü h n , G. He n s e l , H. Gn a d , J. We y e n , J. Ku m l e h n & F.R. Bl a t t n e r , L. Al t s c h m i e d , G. Ha b e r e r , G. Ba r c a c c i a & H. Bä u m - K. Pi l l e n : An investigation of transgenic lines of culti- l e i n : Identification and genetic analysis of theA POSPORY vated spring barley (Hordeum vulgare L.) carrying flow- locus in Hypericum perforatum L. – XXI. International ering genes of wild barley (Hordeum vulgare ssp. spon- Congress on Sexual Plant Reproduction, Bristol/UK, taneum). – Conference on Genomics-Based Breeding of 02.-06.08.2010. the GPZ Ag Genome Analysis, Justus-Liebig-Universität, P223. Sc h a l l a u , K., S.M. Ba k e r , T. Liiving, I. Fr e i e r , S. We b e r & Gießen, 26.-28.10.2010. B. Ju n k e r : Data-driven kinetic modelling of metabolic P211. Ro h n , H., C. Kl u k a s & F. Sc h r e i b e r : Integrative visualiza- pathways involved in storage compound synthesis. – tion of multimodal biological data. – VIZBI Workshop, Institutstag IPK, Gatersleben, 04.-05.10.2010. Heidelberg, 03.-05.03.2010. P224. Sc h l i c h t i n g , A., K.J. De h m e r , R. Mü l l e r & P. Le i n w e b e r : The P212. Ro h n , H., C. Kl u k a s & F. Sc h r e i b e r : Integrative visualization chemical composition of potato tubers: Profiling of of multimodal biological data. – 6th International Sym- quality parameters to evaluate genetic resources. – 7th posium on Integrative Bioinformatics 2010, Cambridge/ Solanaceae Conference (SOL2010), Dundee, Scotland/ UK, 22.-24.03.2010. UK, 05.-09.09.2010. P213. Ro l l e t s c h e k , H., D. Ma c h e r e l & L. Bo r i s j u k : Transient hypox- P225. Sc h l ü t e r , U., A. Fe r n i e , A. We b e r , F. Sc h r e i b e r , F. Bö r n k e , H. ia during seed germination: the role of mitochondria. Fa h n e n s t i c h , M. Bu c h e r , T. Dr e s s e l h a u s , U. Sc h o l z & U. So n - – Arbeitstagung der Arbeitsgemeinschaft Saatgut- und n e w a l d : OPTIMAS – Optimisation of biomass produc- Sortenwesen der GPZ und GPW, IPK, Gatersleben, 24.- tion in maize. – 10th GABI Status Seminar, Potsdam, 09.- 25.02.2010. 11.03.2010. P214. Ro l l e t s c h e k , H., G. Me l k u s , S. Sc h i e b o l d , N. He i n z e l , H. Ts c h i e r s c h , P226. Sc h m i d , N.: PLANT-KBBE: HOT IRON Approach for the iden- J. Ja s i k , J. Fu c h s & T. Al t m a n n : Modelling of oil storage in crop tification of genes improving iron efficiency in Arabidop- seeds. – Institutstag IPK, Gatersleben, 04.-05.10.2010. sis. – 10th GABI Status Seminar, Potsdam, 09.-11.03.2010. P215. Ro l l e t s c h e k , H., J. Th i e l , H.A. Ng u y e n & L. Bo r i s j u k : Seed- P227. Sc h m i d , N.: A role of the SNARE protein VTI11 in intracel- specific upregulation of hemoglobins affects endoge- lular iron efficiency in Arabidopsis thaliana. – 15th Inter- nous nitric oxide, mitochondrial respiration and more. national Symposium on Iron Nutrition and Interactions – 24th New Phytologist Symposium “Plant respiration in Plants, Budapest/Hungary, 26-30.06.2010.

195 Vorträge und Poster/Lectures and Posters

P228. Sc h m i d , N.: A role of the SNARE protein VTI11 in intracel- P239. Seidensticker , T. & T. Sc h n u r b u s c h : Towards the molecular lular iron efficiency in Arabidopsis thaliana. – Deutsche elucidation of ‘Miracle Wheat’ – wheats with branched Gesellschaft für Pflanzenernährung (DGP) Symposium, inflorescences. – 10th GABI Status Seminar, Potsdam, Hannover, 30.09.-02.10.2010. 09.-11.03.2010. P229. Sc h m i d t , R. & A. Boudichevskaia : GABI-OIL Omics-based P240. Seidensticker , T. & T. Sc h n u r b u s c h : Towards the molecular strategies for increasing seed oil content of rapeseed: elucidation of ‘Miracle Wheat’ – wheats with branched analysis of genetic diversity in Brassica napus. – 10th inflorescences. – th6 Plant Science Student Conference, GABI Status Seminar, Potsdam, 09.-11.03.2010. IPK, Gatersleben, 15.-18.06.2010. P230. Sc h m u t z e r , T., B. St e u e r n a g e l , F. Bu l l , S. Kl e m m e , A. Ho u b e n , P241. Se i f e r t , M., A.M. Ba n a e i Mo g h a d d a m , A. Go h r , M. St r i c k e r t N. St e i n & U. Sc h o l z : K-mer analysis to reveal genomic & I. Gr o s s e : Parsimonious higher-order Hidden Markov ambiguities in highly repetitive genomes. – Genome Models for enhanced comparative genomics. – 9th Euro- Informatics 2010, Wellcome Trust Conference Centre, pean Conference on Computational Biology (ECCB Hinxton/UK, 15.-19.09.2010. 2010), Ghent/Belgium, 26.-29.09.2010. P231. Sc h m u t z e r , T., B. St e u e r n a g e l , F. Bu l l , S. Kl e m m e , A. Ho u b e n , N. P242. Se i f e r t , M., A.M. Ba n a e i Mo g h a d d a m , A. Go h r , M. St r i c k e r t St e i n & U. Sc h o l z : K-mer analysis to reveal genomic am- & I. Gr o s s e : Parsimonious higher-order Hidden Markov biguities in highly repetitive plant genomes. – Instituts- Models for enhanced comparative genomics. – From tag IPK, Gatersleben, 04.-05.10.2010. Functional Genomics to Systems Biology, EMBL, Heidel- P232. Sc h m u t z e r , T., B. St e u e r n a g e l , F. Bu l l , S. Kl e m m e , A. Ho u b e n , berg, 13.-16.11.2010. N. St e i n & U. Sc h o l z : K-mer analysis to reveal genomic P243. Se i f e r t , M., M. St r i c k e r t , A. Sc h l i e p & I. Gr o s s e : Analysis of tu- ambiguities in highly repetitive genomes. – 10th Gaters- mor gene expression data by integrating chromosomal leben Research Conference 2010 (GRCX) “Sequence- distances of genes into HMMs. – 12th Status Seminar informed Crop Research”, Quedlinburg (Palais Salfeldt)/ “Chip Technologies, Sequencing and Functional Ge- Gatersleben (IPK), 22.-24.11.2010. nomics”, Frankfurt am Main, 04.-05.02.2010. P233. Sc h r e i b e r , F., S. We i s e , C. Co l m s e e , E. Gr a f a h r e n d -Be l a u , P244. Se i f f e r t , U., F. Bo l l e n b e c k , H.-P. Mo c k & A. Ma t r o s : Clustering B.H. Ju n k e r , C. Kl u k a s , D. Ko s c h ü t z k i , M. La n g e & U. Sc h o l z : of crop phenotypes by means of hyperspectral signa- SBGN-ED for editing, validating, and translating of SBGN tures using artificial neural networks. – 2nd Workshop in Maps and application examples. – 11th International Hyperspectral Image and Signal Processing: Evolution Conference on Systems Biology (ICSB 2010), Edinburgh, in Remote Sensing (IEEE-WHISPERS), Reykjavik/Iceland, Scotland/UK, 10.-15.10.2010. 14.-17.06.2010. P234. Sc h r o e d e r , I.S., S. Su l z b a c h e r , J. Fu c h s , T. No l d e n , H. Hi m m e l b a u e r , P245. Se i l e r , C., V.T. Harshavardhan , K. Ra j e s h , U. Sc h o l z , M. Lo h s e , J. Cz a r n o t a , R. Me i s t e r f e l d & A.M. Wo b u s : Potential of Sox17- B. Us a d e l , U. Wo b u s & N. Sreenivasulu : ABA biosynthesis expressing mouse embryonic stem cells induced to dif- and degradation during barley seed development un- ferentiate into endoderm and early pancreatic lineages der control and terminal drought stress conditions. – by activin A. – 3rd International Congress on Stem Cells 10th GABI Status Seminar, Potsdam, 09.-11.03.2010. and Tissue Formation, Dresden, 11.-14.07.2010. P246. Se n u l a , A., D. Bü c h n e r & E.R.J. Ke l l e r : Dehydrating pre- P235. Sc h r o e d e r , I.S., S. Su l z b a c h e r , J. Fu c h s , T. No l d e n , H. Him- treatment, water relations and survival of shallot shoot m e l b a u e r , J. Ca r n o t h a , R. Me i s t e r f e l d & A.M. Wo b u s : Po- tips from field and in vitro culture. – 47th Annual Meet- tential of Sox17-expressing mouse embryonic stem ing of the Society for Cryobiology (CRYO-2010), Bristol/ cells induced to differentiate into the endoderm and UK, 17.-20.07.2010. early pancreatic lineage by activin A. – Institutstag IPK, P247. Se n u l a , A., D. Bü c h n e r & E.R.J. Ke l l e r : Dehydrating pre- Gatersleben, 04.-05.10.2010. treatment, water relations and survival of shallot shoot P236. Sc h u b e r t , I., K. Wa t a n a b e , J. Fu c h s & V. Sc h u b e r t : Interphase tips from field and in vitro culture. – Institutstag IPK, chromosome arrangement, homologous recombination Gatersleben, 04.-05.10.2010. repair and mitotic chromosome segregation are impaired P248. Se y f a r t h , M., K. We i g e l t , M. Er n s t , R.C. Me y e r & T. Al t m a n n : in cohesin mutants. – EMBO Workshop on Chromosome Identification and characterization of functional diversi- Segregation and Aneuploidy, Edinburgh, Scotland/UK, ty of regulatory proteins controlling plant growth. – Bio- 19.-23.06.2010. wissenschaftliches Netzwerk „Strukturen und Mecha- P237. Sc h w a r z , M., E. Bö e r , H.-M. Vo r b r o d t , M. Pi o n t e k & G. Ku n z e : nismen der biologischen Informationsverarbeitung“, Glycosidase as biocatalyst for the establishment of su- Gesamtarbeitsbesprechung, Martin-Luther-Universität gar derivates for biomedicine and biotechnology. – 6th Halle-Wittenberg, Halle/S., 04.-05.03.2010. Plant Science Student Conference, IPK, Gatersleben, 15.- P249. Sh a h i n n i a , F., A. Dr u k a , R. Wa u g h , M. Mo r g a n t e & N. St e i n : 18.06.2010. Positional cloning of the gene dense spike (dsp1) on P238. Sedzielewska , K., D. Kl e m a n n , N. Nü r k , J. Fu c h s , K. Fl o r s c h ü t z , barley chromosome 7H by utilization of next genera- A. Sc h r ö t e r , K. Wa t z k e & G. Ku n z e : Genomic and phylo- tion grass-genomic tools. – Plant and Animal Genome genetic characterization of symbiotic glomeromycota XVIII. Conference, San Diego/USA, 09.-13.01.2010. Glomus intraradices. – Institutstag IPK, Gatersleben, 04.-05.10.2010.

196 P250. Sh a r m a , R., R. Pa s a m , B. Kilian & A. Gr a n e r : Genome-wide P262. Th i e l , J., D. We i e r & W. We s c h k e : Microdissection-based association studies for plant architectural traits in differ- studies of young barley seeds reveal specific regulatory ent barley gene pools. – Institutstag IPK, Gatersleben, programmes and function in nucellar projection and 04.-05.10.2010. endosperm transfer cells. – International Conference P251. Sh a r m a , R., R.K. Pa s a m , B. Kilian & A. Gr a n e r : Whole genome “Molecular Aspects of Plant Development”, Vienna/Aus- association studies for plant architecture traits in differ- tria, 23.-26.02.2010. ent gene pools of barley. – Conference on Genomics- P263. Th i e l , T., J. Ho l m a n n , T. Sc h m u t z e r , U. Sc h o l z , H. We b e r & Based Breeding of the GPZ Ag Genome Analysis, Justus- W. We s c h k e : The transcriptome of the differentiating Liebig-Universität, Gießen, 26.-28.10.2010. barley grain transfer region: new sequence information P252. Sp r i n g m a n n , C., S. Hö r d l i n g , T. Me c h a n i k , S. St r u b e , F. Ba k o s from microdissection-based 454 sequencing. – Instituts- & J. Ku m l e h n : Development of commercially applicable tag IPK, Gatersleben, 04.-05.10.2010. methods to generate DHLs in cereals. – 10th GABI Status P264. Th i e l , T., D. We i e r , B. Ha u s e , H. We b e r & W. We s c h k e : Gradients Seminar, Potsdam, 09.-11.03.2010. of ABA biosynthesis and distribution in developing barley P253. St a r o s k e , N., V. Ra d c h u k , G. He n s e l , J. Ku m l e h n , W. We s c h k e grains. – Institutstag IPK, Gatersleben, 04.-05.10.2010. & U. Co n r a d : Immunomodulation of abscisic acid in de- P265. Ti k h e n k o , N., N. Ts v e t k o v a , A. Vo y l o k o v & A. Bö r n e r : Wheat veloping barley grains. – International Conference “Mo- improvement using rye – disturbance by embryo le- lecular Aspects of Plant Development”, Vienna/Austria, thality. – 8th International Wheat Conference, St. Peters- 23.-26.02.2010. burg/Russia, 01.-04.06.2010. P254. St a r o s k e , N., V. Ra d c h u k , G. He n s e l , J. Ku m l e h n , W. We s c h k e & P266. Tr a u t w e i n , A., D. Ja n k o w s k a , A. Co r d e s & G. Ku n z e : Purine U. Co n r a d : Immunomodulation of abscisic acid in devel- degradation pathway in Arxula adeninivorans – Urate oping barley grains. – 10th GABI Status Seminar, Pots- oxidase as an additive to reduce the uric acid content dam, 09.-11.03.2010. of food. – 6th Plant Science Student Conference, IPK, P255. St e u e r n a g e l , B., T. Sc h m u t z e r , S. Ta u d i e n , T. Wi c k e r , M. Pl a t z e r , Gatersleben, 15.-18.06.2010. K.F.X. Ma y e r , U. Sc h o l z & N. St e i n : Evaluation of assemblers P267. Tr ö s c h , M., I. Ki l b i e n s k i , A. Sc h ä f e r , I. Ha j d a s , G. He n s e l , J. Ku m - on 454 data of highly repetitive eukaryotic sequences. l e h n & K. Kr u p i n s k a : The nuclear protein HvS40 controls – Plant and Animal Genome XVIII. Conference, San Die- leaf senescence in barley. – 5th European Plant Senes- go/USA, 09.-13.01.2010. cence Network Meeting (SENnet 2010), Wokefield Park, P256. St e u e r n a g e l , B., T. Sc h m u t z e r , S. Ta u d i e n , M. Pl a t z e r , K.F.X. Ma y e r , Reading/UK, 31.08.-03.09.2010. T. Wi c k e r , U. Sc h o l z & N. St e i n : Evaluation of assembler on P268. v a n Ie r s e l , M.P., S.E. Bo y d , F. Be r g m a n n , S. Mo o d i e , F. Sc h r e i b e r , 454 data of highly repetitive eukaryotic sequences. – 10th T. Cz a u d e r n a , E. De m i r , N. Le No v è r e , A. So r o k i n , H. Mi, GABI Status Seminar, Potsdam, 09.-11.03.2010. A. Lu n a , U. Do g r u s o z , Y. Ma t s u o k a , A. Fu n a h a s h i , H. Ki t a n o , P257. St e u e r n a g e l , B., T. Sc h m u t z e r , S. Ta u d i e n , M. Fe l d e r , A. Pe t z o l d , M.I. Al a d j e m , M.L. Bl i n o v & A.C. Vi l l é g e r : LibSBGN: elec- M. Pl a t z e r , R. Ar i y a d a s a , N. St e i n & U. Sc h o l z : Utilizing next- tronic exchange of SBGN maps. – 11th International generation sequencing of barcoded BAC pools to ana- Conference on Systems Biology (ICSB 2010), Edinburgh, lyze the complex genome of barley. – Next Generation Scotland/UK, 10.-15.10.2010. Sequencing Symposium 2010, Max-Planck-Institut für P269. Wa t a n a b e , K., J. Ku m l e h n & I. Sc h u b e r t : Gene targeting and Züchtungsforschung, Köln, 03.-05.11.2010. recombination repair in barley using I-SceI endonuclease. P258. St o l l , A. & F.R. Bl a t t n e r : Diversidad genetica y filogeogra- – SEB (Society for Experimental Biology) Annual Main fia de Nothofagus dombeyi (Mirb.) Oerst en Chile. – X Meeting, Prague/Czech Republic, 30.06.-03.07.2010. Congresso Latinoamericano de Botanica, La Serena/ P270. Wa t a n a b e , K., J. Ku m l e h n & I. Sc h u b e r t : Gene targeting and Chile, 04.-10.10.2010. recombination repair in barley using I-SceI endonu- P259. Te o , C.H., L. Ma, E. Ka p u s i , G. He n s e l , A. Ho u b e n , J. Ku m l e h n , clease. – Institutstag IPK, Gatersleben, 04.-05.10.2010. I. Sc h u b e r t & M.F. Me t t e : Construction of engineered P271. We i g e l t , K., R.C. Me y e r , D. Ri e w e , B. Eb e r t , J. Li s e c , R. Sc h m i d t , minichromosomes in barley (Hordeum vulgare). – SEB L. Wi l l m i t z e r & T. Al t m a n n : GABI-GNADE: Genome-wide (Society for Experimental Biology) Annual Main Meet- natural diversity exploitation in the Arabidopsis popu- ing, Prague/Czech Republic, 30.06.-03.07.2010. lation; identification and characterization of genes P260. Te o , C.H., L. Ma, E. Ka p u s i , G. He n s e l , A. Ho u b e n , J. Ku m l e h n , controlling vegetative biomass accumulation in Arabi- I. Sc h u b e r t & M.F. Me t t e : Construction of engineered dopsis thaliana. – 10th GABI Status Seminar, Potsdam, minichromosomes in barley (Hordeum vulgare). – Insti- 09.-11.03.2010. tutstag IPK, Gatersleben, 04.-05.10.2010. P272. We i g e l t , K., R.C. Me y e r , D. Ri e w e , B. Eb e r t , J. Li s e c , R. Sc h m i d t , P261. Te o , C.H., L. Ma, E. Ka p u s i , G. He n s e l , A. Ho u b e n , J. Ku m l e h n , L. Wi l l m i t z e r & T. Al t m a n n : Identification and character- I. Sc h u b e r t & M.F. Me t t e : Construction of engineered ization of genes controlling vegetative biomass ac- minichromosomes in barley (Hordeum vulgare). – 10th cumulation in Arabidopsis thaliana. – 21st International Gatersleben Research Conference 2010 (GRCX) “Se- Conference on Arabidopsis Research, Yokohama/Japan, quence-informed Crop Research”, Quedlinburg (Palais 06.-10.06.2010. Salfeldt)/Gatersleben (IPK), 22.-24.11.2010.

197 Vorträge und Poster/Lectures and Posters

P273. We i s s h a a r , C., S. Sp a e p e n , J. Vanderleyden , J. St r a u s s & N. v o n P8. Ba l i n t , A.F., F. Sz i r a & A. Bö r n e r : Identification of loci af- Wi r é n : Influence of associative bacteria on root morphol- fecting grain micronutrient content in cereals using ogy in Arabidopsis plants. – 1st International Symposium association mapping. – Agrisafe Final Conference “Cli- on the Nitrogen Nutrition of Plants (Nitrogen2010), mate change: challenge for training of applied plant Inuyama International Sightseeing Center “FREUDE”, scientists”, Budapest/Hungary, 21.-23.03.2011. Aichi/Japan, 26.-30.07.2010. P9. Ba n a e i Mo g h a d d a m , A.M., F. Ro u d i e r , M. Se i f e r t , C. Be r a r d , P274. Wi c k e r , T., N. St e i n , J.P. Bu c h m a n n & B. Ke l l e r : The dy- M.L. Ma g n i e t t e , R. Ka r i m i As h t i y a n i , A. Ho u b e n , V. Co l o t & namic equilibrium of genome size in plants. – Plant M.F. Me t t e : Dynamics of histone modifications H3K4me2 and Animal Genome XVIII. Conference, San Diego/USA, and H3K27me3 in Arabidopsis thaliana in response to in- 09.-13.01.2010. traspecies hybridization. – EMBO Conference Series “Chro- P275. Wi c k e r , T., N. St e i n , T. Ko m a t s u d a , J.P. Bu c h m a n n & B. Ke l l e r : matin and Epigenetics”, EMBL, Heidelberg, 01.-05.06.2011. Rapid evolution of intergenic regions in plants: a rich P10. Ba n a e i Mo g h a d d a m , A.M., S. Kl e m m e , V. Sc h u b e r t & A. Ho u b e n : source of polymorphisms. – Plant and Animal Genome Drive mechanism of a selfish chromosome. – 18th Inter- XVIII. Conference, San Diego/USA, 09.-13.01.2010. national Chromosome Conference, University Place, P276. Wi l l n e r, E., M. Op p e r m a n n & K.J. De h m e r : Provision of grass Manchester/UK, 29.08.-02.09.2011. C & E data via GBIS for a more efficient PGR utilization – P11. Ba r u n a w a t i , N., B. Ba u e r & N. v o n Wi r é n : Influence of foliar Institutstag IPK, Gatersleben, 04.-05.10.2010. citric acid application on micronutrient remobilization P277. Wo r c h , S., N. Sreenivasulu , K. Ra j e s h , L. Ku n t z e & M.S. Rö d e r : and retranslocation in wheat plants and their depen- Candidate genes for grain yield and quality under termi- dence on nitrogen fertilization. – Botanikertagung 2011 nal drought stress in barley. – 10th Gatersleben Research “Diversity makes the Difference”, Berlin, 18.-23.09.2011. Conference 2010 (GRCX) “Sequence-informed Crop Re- P12. Ba u e r , E., G. Ha s e n e y e r , T. Sc h m u t z e r , M. Se i d e l , R. Zh o u , C.C. search”, Quedlinburg (Palais Salfeldt)/Gatersleben (IPK), Sc h ö n , K.F.X. Ma y e r , U. Sc h o l z & N. St e i n : Bringing rye ge- 22.-24.11.2010. nomics on track: transcripts, SNPs, maps, and diversity. – Plant and Animal Genome XIX. Conference, San Die- go/USA, 15.-19.01.2011. 2011 P13. Bo h n e r , A. & N. v o n Wi r é n : Physiological functions of the urea transporter AtDUR3 in senescing leaves of Arabi- P1. Ah k a m i , A.H., T. Ru t t e n , M. Me l z e r , B. Ha u s e , N. v o n Wi r é n , dopsis. – Botanikertagung 2011 “Diversity makes the P. Fr a n k e n , U. Dr u e g e & M.R. Ha j i r e z a e i : Relationship be- Difference”, Berlin, 18.-23.09.2011. tween auxin transport, auxin accumulation, primary P14. Bo h n e r , A., L. Un r a t h , B. Ke t t i g & N. v o n Wi r é n : Role of ni- metabolism and adventitious root formation in Petunia trogen forms in nitrogen retranslocation during senes- shoot tip cuttings. – Botanikertagung 2011 “Diversity cence – Institutstag IPK, Gatersleben, 04.-06.10.2011. makes the Difference”, Berlin, 18.-23.09.2011. P15. Bo r i s j u k , L. & T. Ne u b e r g e r : Increased throughput for P2. Al i y u , O.M., M. Se i f e r t , J.M. Co r r a l & T.F. Sh a r b e l : Copy quantitative determination of lipid in crop seeds using number variation and apomixis expression in the genus high resolution MRI. – 11th International Conference on Boechera (Brassicaceae). – Institutstag IPK, Gatersleben, Magnetic Resonance Microscopy, Changping, Beijing/ 04.-06.10.2011. China, 14.-18.08.2011. P3. Al q u d a h , A.M., B. Kilian, A. Gr a n e r & T. Sc h n u r b u s c h : Study P16. Bö r n e r , M., M. Na g e l , K. Ne u m a n n , U. Lo h w a s s e r & A. Bö r n e r : of pre-anthesis development in barley – Institutstag Management of plant genetic resources – examples IPK, Gatersleben, 04.-06.10.2011. on seed longevity. – International Conference “Wheat P4. Am i t e y e , S. & T.F. Sh a r b e l : Do microRNAs cause loss of Genetic Resources and Genomics”, Novosibirsk/Russia, sex in Boechera? – 9th IMPRS Symposium, Dornburg, 28.08.-01.09.2011. 15.02.2011. P17. Boudichevskaia , A., J. Ko p p o l u & R. Sc h m i d t : Genetic analysis P5. Ar i y a d a s a , R., D. Sc h u l t e , N. Poursarebani , R. Zh o u , B. St e u e r - of seed traits in Arabidopsis thaliana. – 11th GABI Status n a g e l , H. Liu, P.E. He d l e y , H. Gu n d l a c h , T. Nu s s b a u m e r , P. La n - Seminar, Potsdam, 15.-17.03.2011. g r i d g e , K.F.X. Ma y e r , U. Sc h o l z , A. Gr a n e r & N. St e i n : Whole P18. Br a s s a c , J., S.S. Ja k o b & F.R. Bl a t t n e r : Analysis of phyloge- genome physical map of barley (Hordeum vulgare L.). – netic relationships of Hordeum (Poaceae) polyploids. 11th GABI Status Seminar, Potsdam, 15.-17.03.2011. – 7th Plant Science Student Conference, IPB, Halle/S., P6. Ar i y a d a s a , R., D. Sc h u l t e , N. Poursarebani , R. Zh o u , K.F.X. Ma y - 14.-17.06.2011. e r , R. Wa u g h , M. Pl a t z e r , B. St e u e r n a g e l , U. Sc h o l z , P. La n g r i d g e , P19. Br a s s a c , J., S.S. Ja k o b & F.R. Bl a t t n e r : Analysis of phyloge- T. Cl o s e , A. Gr a n e r & N. St e i n : A physical map of the barley netic relationships of polyploid Hordeum species. – In- genome. – Institutstag IPK, Gatersleben, 04.-06.10.2011. stitutstag IPK, Gatersleben, 04.-06.10.2011. P7. Ba i e r , C. & F.R. Bl a t t n e r : Population genetic structure of P20. Bu c h m a n n , J., M. Ma t s u m o t o , N. St e i n , B. Ke l l e r & T. Wi c k e r : The the obligate myrmecophyte Macaranga winkleri and Mites, they are a-changing’ – how DNA-transposon activ- the non-myrmecophytic M. tanarius (Euphorbiaceae). – ity corrodes collinearity. – Plant and Animal Genome XIX. BioSystematics, Berlin, 21.-27.02.2011. Conference, San Diego/USA, 15.-19.01.2011.

198 P21. Bu n d t z e n , S., K. Sc h a l l a u & B.H. Ju n k e r : Metabolic engineer- Martin-Luther-Universität Halle-Wittenberg, Halle/S., ing based on computational models for starch produc- 28.-30.03.2011. tion in seeds. – Botanikertagung 2011 “Diversity makes P34. De m i d o v , D., O. We i s s , J. Fu c h s & A. Ho u b e n : Cross-talk be- the Difference”, Berlin, 18.-23.09.2011. tween histone H3 phosphorylation per AtAurora ki- P22. Ca o , H.X. & R. Sc h m i d t : PCR-based screening platform for an nases and other post-translational histone modifica- oilseed rape BAC library. – Institutstag IPK, Gatersleben, tions in Arabidopsis. – Proteomic Forum 2011, Freie Uni- 04.-06.10.2011. versität Berlin, 03.-07.04.2011. P23. Ch e n , W. & P. Sc h w e i z e r : Establishment of a virus-induced P35. De m i d o v , D., S. He c k m a n n , I. Le r m o n t o v a , T. Ru t t e n , J. Fu c h s gene silencing system in adult wheat plants for Fusarium & A. Ho u b e n : Physiological function of Aurora kinases head blight interactions. – SFB 648 International Meeting and other spindle assembly checkpoint (SAC) proteins “Communication in Plants and their Responses to the En- in plants. – International Meeting “Communication in vironment”, Halle/S., 19.-21.05.2011. Plants and their Responses to the Environment”, Mar- P24. Ch e n , W. & P. Sc h w e i z e r : A virus-induced gene silencing sys- tin-Luther-Universität Halle-Wittenberg, Halle/S., 19.- tem in adult wheat plants for Fusarium head-blight inter- 22.05.2011. actions. – Institutstag IPK, Gatersleben, 04.-06.10.2011. P36. De m i d o v , D., O. We i s s , I. Le r m o n t o v a , T. Ru t t e n , J. Fu c h s , P25. Ch e s n o k o v , Y.V., E.A. Go n c h a r o v a , M.N. Sitnikov, N.B. Po c h e p n y a , T.F. Sh a r b e l & A. Ho u b e n : Physiological function of Aurora N.V. Ko c h e r i n a , U. Lo h w a s s e r & A. Bö r n e r : Mapping of agro- kinases and other spindle assembly checkpoint (SAC) nomically important QTLs in soft spring wheat (Triticum proteins in plants. – EMBO Conference Series “Chroma- aestivum L.) in dose gradient of nitrogen nutrition. – tin and Epigenetics”, EMBL, Heidelberg, 01.-05.06.2011. EWAC-EUCARPIA Cereals Section Conference, Novi Sad/ P37. Di e k m a n n , K. & K.J. De h m e r : Management of the IPK po- Serbia, 07.-11.11.2011. tato collections using molecular markers. – Institutstag P26. Cl o s e , T.J., A. Gr a n e r , P. La n g r i d g e , C. Li, M. Ma t s u m o t o , IPK, Gatersleben, 04.-06.10.2011. G. Mu e h l b a u e r , K. Sa t o , A. Sc h u l m a n , R. Wa u g h , R. Wi s e , P38. Di t t b r e n n e r , A., H. Ne u w e g e r & H.-P. M o c k : Data mining G. Zh a n g & N. St e i n : International Barley Genome Sequenc- for GC-MS based metabolite profiling using MeltDB. – ing Consortium – the barley genome is in sight. – Plant DGMS-Tagung, Dortmund, 27.02.-02.03.2011. and Animal Genome XIX. Conference, San Diego/USA, P39. Di t t b r e n n e r , A. & H.-P. Mo c k : Anthocyanin profiling of 15.-19.01.2011. different plant materials and preparative isolation. – P27. Cz a u d e r n a , T., C. Kl u k a s & F. Sc h r e i b e r : Editing, validating, and 5th International Conference on Polyphenols and Health translating of SBGN maps with SBGN-ED. – International (ICPH), Barcelona/Spain, 17.-20.10.2011. Symposium on Integrative Bioinformatics 2011 (IB2011), P40. Di t t m a n n , C., M. Op p e r m a n n & H. Kn ü p f f e r : Conceptual Wageningen/The Netherlands, 21.-23.03.2011. integration of data acquisition and management pro- P28. Cz a u d e r n a , T., C. Kl u k a s & F. Sc h r e i b e r : Editing, validating, cesses for in vitro preservation in the genebank infor- and translating of SBGN maps with SBGN-ED. – HAR- mation system GBIS – Institutstag IPK, Gatersleben, 04.- MONY 2011: The Hackathon on Resources for Modeling 06.10.2011. in Biology, New York/USA, 18.-22.04.2011. P41. Dobrovolskaya , O., P. Ma r t i n e k , L.I. La i k o v a , V.S. Ar b u z o v a , P29. Cz a u d e r n a , T., E. Gr a f a h r e n d -Be l a u , A. Ju n k e r , A. Ha r t m a n n , J. Sa l s e , A. Bö r n e r & E.A. Sa l i n a : Identification and charac- M. Klapperstück & F. Sc h r e i b e r : Novel developments in terization of genes that are involved in regulation of the SBGN-ED and applications. – 12th International Confer- cereal inflorescence development in bread wheat (T. aes- ence on Systems Biology (ICSB2011), Heidelberg/Mann- tivum L.) and its close relatives. – EWAC-EUCARPIA Cere- heim, 28.08.-01.09.2011. als Section Conference, Novi Sad/Serbia, 07.-11.11.2011. P30. Cz a u d e r n a , T., E. Gr a f a h r e n d -Be l a u , A. Ju n k e r , A. Ha r t m a n n , P42. Dö l l , S., D. Be r r e t h & H.-P. Mo c k : An efThoc1 superfamily M. Klapperstück & F. Sc h r e i b e r : Novel developments in SB- transcription factor mediates the enhanced production GN-ED and applications. – COMBINE 2011, Heidelberg, of the coumarin scopolin after abiotic stress. – Instituts- 03.-07.09.2011. tag IPK, Gatersleben, 04.-06.10.2011. P31. Cz a u d e r n a , T., E. Gr a f a h r e n d -Be l a u , A. Ju n k e r , A. Ha r t m a n n , P43. Do n a t h , S. & N. v o n Wi r é n : Effect of endogenous polyamine M. Klapperstück & F. Sc h r e i b e r : Novel developments in SB- levels on leaf senescence in dependence of N status. – GN-ED and applications. – Institutstag IPK, Gatersleben, Botanikertagung 2011 “Diversity makes the Difference”, 04.-06.10.2011. Berlin, 18.-23.09.2011. P32. Da g h m a , D.S., G. He n s e l , T. Ru t t e n , J. Ku m l e h n & M. Me l z e r : P44. Do u c h k o v , D., A. Jo h r d e , A. Hi m m e l b a c h , G. He n s e l , J. Ku m l e h n GABI-POEM – structural study of initial mechanisms of & P. Sc h w e i z e r : Genes and loci for quantitative disease pollen embryogenesis. – 11th GABI Status Seminar, Pots- resistance in barley. – SFB 648 International Meeting dam, 15.-17.03.2011. “Communication in Plants and their Responses to the P33. De m i d o v , D., O. We i s s , I. Le r m o n t o v a , T. Ru t t e n , J. Fu c h s , Environment”, Halle/S., 19.-21.05.2011. T.F. Sh a r b e l & A. Ho u b e n : Function of plant Aurora kinases P45. En t z i a n , A., I. Mü c k e , J.-M. Pa p e & C. Kl u k a s : Automatisierte and other SAC proteins during microsporogenesis. – Analyse von Mais-Pflanzenbildern mit der Software IAP 2nd Annual Meeting of the DFG Priority Programme („Integrated Analysis Platform“). – Tag der offenen Tür, SPP1384 – Mechanisms of Genome Haploidization, IPK, Gatersleben, 28.05.2011.

199 Vorträge und Poster/Lectures and Posters

P46. En t z i a n , A., J.-M. Pa p e & C. Kl u k a s : Automated analysis of tection of phytopathogenic viruses and bacteria as well crop plant images with the software IAP „Integrated as receptor interactions. – Institutstag IPK, Gatersleben, Analysis Platform“. – 2nd International Plant Phenotyp- 04.-06.10.2011. ing Symposium, Jülich, 05.-07.09.2011. P58. Fr a n k e , M., M.R. Gh a f f a r i , A. Ju n k e r , C. Kr a c h , A. Es c h e n r ö d e r , P47. En t z i a n , A., J.-M. Pa p e & C. Kl u k a s : Automated analysis of T. He r t e r , H. Ri e g l e r , K. Kn o r r , K. Lo t z , M. Ha j i r e z a e i , B.H. Ju n k e r , crop plant images with the Integrated Analysis Platform M. Mü l l e r, B. Us a d e l , M. Le p s & F. Sc h r e i b e r : BioEnergie 2021 – IAP. – Institutstag IPK, Gatersleben, 04.-06.10.2011. MMM – Multiscale Metabolic Modelling of cereals: An in- P48. En t z i a n , A., J.-M. Pa p e & C. Kl u k a s : Automated maize tegrative systems biology approach for biomass research. phenotyping. – International Symposium “Phenodays – 11th GABI Status Seminar, Potsdam, 15.-17.03.2011. 2011”, Wageningen/The Netherlands, 12.-14.10.2011. P59. Fr e i s t , S., I. Ot t o , G. He n s e l , K. Boutilier, T.F. Sh a r b e l & P49. Er m a k o v a , M.F., A.K. Ch i s t y a k o v a , L.V. Sh c h u k i n a , T.A. Ps h e - J. Ku m l e h n : Genetic transformation of Boechera polyan- n i c h n i k o v a , E.V. Mo r o z o v a , V. Si m o n o v , A. We i d n e r & A. Bö r n - tha (formerly B. holboellii): The way forward to function- e r : Technological properties of grain and flour in bread ally characterize genes involved in apomictic reproduc- wheat lines with introgressions from Aegilops speltoides tion. – Institutstag IPK, Gatersleben, 04.-06.10.2011. and Aegilops markgrafii. – Agrisafe Final Conference P60. Fu c h s , J., G. Me l k u s , L. Bo r i s j u k & P.M. Ja k o b : Imaging “Climate change: challenge for training of applied plant of metabolite dynamics in plant seeds using IVD. – scientists”, Budapest/Hungary, 21.-23.03.2011. 26th International Conference on Low Temperature P50. Er m a k o v a , M.F., A.K. Ch i s t y a k o v a , L.V. Sh c h u k i n a , T.A. Ps h e - Physics (ICMRU 2011), Beijing/China, 10.-17.08.2011. n i c h n i k o v a , E.V. Mo r o z o v a , A.V. Si m o n o v , A. We i d n e r & P61. Fu c h s , J., G. Me l k u s , L. Bo r i s j u k & P.M. Ja k o b : Spectroscopic A. Bö r n e r : Technological properties of grain and flour imaging of metabolite dynamics in plant seeds using in bread wheat lines with introgressions from Aegilops Interleaved Variable Density (IVD) acquisition location. speltoides and Aegilops markgrafii. – International Con- – 11th International Conference on Magnetic Resonance ference “Wheat Genetic Resources and Genomics”, No- Microscopy, Changping, Beijing/China, 14.-18.08.2011. vosibirsk/Russia, 28.08.-01.09.2011. P62. Ge r l a c h , T., C. Ch e r k o u k , L. Re b o h l e , S. Ho w i t z , G. Ha n k e , P51. Fe i x , B., V. Ra d c h u k , A. Ne r l i c h , C. Hü m e r , R. Ra d c h u k , P. Le h m a n n , M. Jä h n e , K. Si m o n , S. Uh l i g & G. Ku n z e : Rezeptor- R.J.N. Em e r y , H. Ke l l e r , K.-P. Gö t z , W. We s c h k e , P. Geigenberger selektive Anreicherung und Bio-LED-Sensorik zur quali- & H. We b e r : Barley grains, deficient in the cytosolic small tativen und quantitativen Detektion von östrogen- und subunit of ADP-Glucose pyrophosphorylase, reveal co- androgen-wirkenden Substanzen. – 7. Deutsches Bio- ordinated down-regulation of C:N metabolism medi- sensorsymposium, Heiligenstadt, 03.-06.04.2011. ated by an overlapping metabolic-hormonal control – P63. Ge r s o n , L., K.J. De h m e r & S. Ha r t m a n n : Elucidation of the Institutstag IPK, Gatersleben, 04.-06.10.2011. genetic diversity in populations of perennial ryegrass P52. Fi n k e , A., M. Ku h l m a n n & M.F. Me t t e : Genetic analysis of and development of selection methods for the trait RNA-directed transcriptional gene silencing in Ara- “persistence“. – 7th Plant Science Student Conference, bidopsis. – 7th Plant Science Student Conference, IPB, IPB, Halle/S., 14.-17.06.2011. Halle/S., 14.-17.06.2011. P64. Ge r s o n , L., A. Ha r t m a n n , M. Pr o b s t , S. Se e f e l d e r & K.J. De h - P53. Fi n k e , A., M. Ku h l m a n n & M.F. Me t t e : Genetic analysis of m e r : Analysis of allele compositions and development of RNA-directed transcriptional gene silencing in Arabi- selection methods. – Botanikertagung 2011 “Diversity dopsis thaliana. – ESF-EMBO Symposium “Epigenetics makes the Difference”, Berlin, 18.-23.09.2011. in Context: From Ecology to Evolution”, Sant Feliu de P65. Gh a f f a r i , M.R. & M.R. Ha j i r e z a e i : Based prediction for bio- Guixols/Spain, 18.-23.09.2011. mass formation during grain filling in barley. –I nstituts- P54. Fi n k e , A., M. Ku h l m a n n & M.F. Me t t e : Genetic analysis of tag IPK, Gatersleben, 04.-06.10.2011. RNA-directed transcriptional gene silencing in Ara- P66. Gi e r s b e r g , M., U. Hä h n e l , S. Wo r c h , P. Mi n h Ha, A. Tr a u t w e i n , D. bidopsis thaliana. – Institutstag IPK, Gatersleben, 04.- Ja n k o w s k a , M. Sc h w a r z & G. Ku n z e : Arxula adeninivorans – a 06.10.2011. valuable tool for basic research and biotechnological ap- P55. Fl o r s c h ü t z , K., A. Sc h r ö t e r , S. Sc h m i e d e r , F. So n n t a g & plication. – Institutstag IPK, Gatersleben, 04.-06.10.2011. G. Ku n z e : On-Chip-Nachweis von phytopathogenen P67. Go v i n d , G., V.T. Harshavadhan , T. Ru t t e n & N. Sreenivasulu : Spike- RNA-Viren mittels Oberflächenplasmonenresonanz – branching and -row phenotype: molecular tools to alter Barley Stripe Mosaic Virus. – Sensor + Test 2011, Nürn- grain number and its potential benefits under terminal berg, 07.-09.06.2011. drought. – Institutstag IPK, Gatersleben, 04.-06.10.2011. P56. Fl o r s c h ü t z , K., A. Sc h r ö t e r , S. Sc h m i e d e r , F. So n n t a g & P68. Gr u b e r , B.D., R.F.H. Gi e h l , E. Fr a u s t & N. v o n Wi r é n : The differen- G. Ku n z e : On-Chip-Nachweis von phytopathogenen tial effect of nutrient deficiencies on the root morphology RNA-Viren mittels Oberflächenplasmonenresonanz of Arabidopsis thaliana. – Botanikertagung 2011 “Diversity – Potato Virus Y. – Sensor + Test 2011, Nürnberg, makes the Difference”, Berlin, 18.-23.09.2011. 07.-09.06.2011. P69. Gr y c z k a , C., M. Er n s t , M.M. Mu r a y a , S. Fr i e d e l , D. Ri e w e , J. Li s e c , P57. Fl o r s c h ü t z , K., A. Sc h r ö t e r , A. Ch a m a s , T. Ge r l a c h , F. So n n t a g , A. Cz e d i k -Ey s e n b e r g , R.C. Me y e r , C. Riedelsheimer , M. St i t t , J. Se l b i g , S. Sc h m i e d e r , U. Kl o t z b a c h , P. Sc h w e i z e r & G. Ku n z e : Phytochip L. Wi l l m i t z e r , A.E. Me l c h i n g e r & T. Al t m a n n : GABI-ENERGY: Bio- and Multiplex Diagnostic Chip – new methods for de- mass production in maize – genomics guided breeding of

200 energy maize and a systems-oriented analysis; genotyp- an integration and analysis pipeline. – Plant Metabolic ing of inbred line panel and phenotyping of inbreds and Engineering Gordon Research Conference, Waterville test crosses under glass house conditions. – 11th GABI Sta- Valley, NH/USA, 24.-29.07.2011. tus Seminar, Potsdam, 15.-17.03.2011. P81. Ha r t m a n n , A., E. Gr a f a h r e n d -Be l a u , A. Ju n k e r , S. We i s e , P70. Gr y c z k a , C., G. He n s e l , D.S. Da g h m a , D. Kö s z e g i , A. Cz i h a l , M. Klapperstück , C. Kl u k a s , H. Ro l l e t s c h e k , L. Bo r i s j u k , L. Al t s c h m i e d , J. Ku m l e h n & H. Bä u m l e i n : Pollen embryogenesis B.H. Ju n k e r , U. Sc h o l z & F. Sc h r e i b e r : Systems biology in related gene expression (GABI-POEM). – 11th GABI Status crop plant metabolism: an integration and analysis pipe- Seminar, Potsdam, 15.-17.03.2011. line. – 12th International Conference on Systems Biology P71. Gu r u s h i d z e , M., E. Ku g e l m a n n , S. Go t t w a l d , N. St e i n & J. Ku m - (ICSB2011), Heidelberg/Mannheim, 28.08.-01.09.2011. l e h n : Instantly homozygous barley mutant lines generated P82. Ha r t m a n n , A., E. Gr a f a h r e n d -Be l a u , A. Ju n k e r , S. We i s e , from EMS-treated embryogenic pollen. – 11th GABI Status M. Klapperstück , C. Kl u k a s , H. Ro l l e t s c h e k , L. Bo r i s j u k , Seminar, Potsdam, 15.-17.03.2011. B.H. Ju n k e r , U. Sc h o l z & F. Sc h r e i b e r : Systems biology in P72. Gu r u s h i d z e , M., F. Bi n i , E. Ku g e l m a n n , S. Go t t w a l d , N. St e i n & crop plant metabolism: an integration and analysis J. Ku m l e h n : Induced mutagenesis in embryogenic pol- pipeline. – Metabolic pathway analysis 2011, University len cultures of barley. – Institutstag IPK, Gatersleben, of Chester, Cheshire, UK, 19.-23.09.2011. 04.-06.10.2011. P83. Ha r t m a n n , A., E. Gr a f a h r e n d -Be l a u , A. Ju n k e r , S. We i s e , P73. Gu s e , T., C. Ma r t h e , E. Gr ü t z e m a n n , J. Tu b i e s & J. Ku m l e h n : Ge- M. Klapperstück , B.H. Ju n k e r , C. Kl u k a s , U. Sc h o l z & F. netic transformation of wheat (Triticum aestivum L.) via Sc h r e i b e r : Systems biology in crop plant metabolism: Agrobacterium tumefaciens infection of in vitro cultured an integration and analysis pipeline. – Institutstag IPK, ovules. – International Conference “Plant Transforma- Gatersleben, 04.-06.10.2011. tion Technologies II”, Vienna/Austria, 19.-22.02.2011. P84. Ha s e n e y e r , G., T. Sc h m u t z e r , M. Se i d e l , R. Zh o u , C.C. Sc h ö n , P74. Ha i l e , J.K., M.M. Na c h i t , K. Ha m m e r , A. Ba d e b o & M.S. Rö d e r : U. Sc h o l z , K.F.X. Ma y e r , N. St e i n & E. Ba u e r : GABI RYE-EX- QTL mapping of resistance to Ethiopian stem rust races PRESS: From RNA-seq to large scale genotyping new of Puccinia graminis f. sp. tritici including Ug99 in durum genomic resources for rye (Secale cereale L.) – 11th GABI wheat (Triticum durum Desf.) – 2011 Field Crop Rust Status Seminar, Potsdam, 15.-17.03.2011. Symposium, San Antonio, Texas/USA, 14.-16.12.2011. P85. Ha s e n e y e r , G., T. Sc h m u t z e r , M. Se i d e l , R. Zh o u , C.C. Sc h ö n , P75. Ha j i r e z a e i , M.R., A.H. Ah k a m i , U. Dr u e g e , B. St e u e r n a g e l , U. Sc h o l z , N. St e i n , K.F.X. Ma y e r & E. Ba u e r : From RNA-seq M. St r i c k e r t , S. Ze r c h e , U. Sc h o l z , N. v o n Wi r é n & P. Fr a n k e n : to large-scale genotyping – genomics resources for rye Microarray-based identification of genes differentially -ex (Secale cereale L.). – Jahrestagung, Technische Univer- pressed in stem base of Petunia hybrida in different devel- sität München, Freising, 16.-18.03.2011. opmental stages of ARF. – 6th International Symposium P86. He c k m a n n , S., K. Na g a k i , E. Sc h r o e d e r -Re i t e r , J. Ma c a s & A. on Root Development, Amos/Canada, 07.-11.08.2011. Ho u b e n : Unusual holocentric chromosomes of the grass P76. Ha n e m a n n , A., S. Sh a r m a , S. Ma r z i n , G. Sc h w e i z e r & M.S. Rö d e r : Luzula elegans. – 2nd Annual Meeting of the DFG Prior- Pectin esterase inhibitor gene: A possible candidate for ity Programme SPP1384 – Mechanisms of Genome Ha- the resistance gene Rrs2 against Rhynchosporium secalis in ploidization, Martin-Luther-Universität Halle-Witten- barley. – International Meeting “Communication in Plants berg, Halle/S., 28.-30.03.2011. and their Responses to the Environment”, Martin-Luther- P87. He c k m a n n , S., I. Le r m o n t o v a , D. De m i d o v , B. Be r c k m a n s , Universität Halle-Wittenberg, Halle/S., 19.-22.05.2011. L. d e Ve y l d e r , H. Bä u m l e i n , I. Sc h u b e r t & A. Ho u b e n : Dynam- P77. Harshavadhan , V., C. Se i l e r , K. Ra j e s h , H. Ro l l e t s c h e k , G. He n - ics of histone modifications H3K4me2 and H3K27me3 s e l , J. Ku m l e h n , U. Wo b u s & N. Sreenivasulu : A transgenics in Arabidopsis thaliana in response to intraspecies hy- strategy for improved seed filling by altering the ABA bridization. – EMBO Conference Series “Histone Variants homeostasis in barley. – 11th GABI Status Seminar, Pots- and Genome Regulation”, IGBMC, Strasbourg/France, dam, 15.-17.03.2011. 01.-05.06.2011. P78. Ha r t m a n n , A., T. Cz a u d e r n a , R. Ho f f m a n n , C. Kl u k a s , T. Al t - P88. He d t m a n n , C., A. Ma t r o s & H.-P. Mo c k : Elucidating the m a n n , F. Sc h r e i b e r & N. St e i n : High-throughput phenotyp- molecular functions of stress-inducible proteins with ing in barley – the IPK plant phenomics facilities. – Plant homology to the human protein HOP in tobacco and and Animal Genome XIX. Conference, San Diego/USA, Arabidopsis. – Botanikertagung 2011 “Diversity makes 15.-19.01.2011. the Difference”, Berlin, 18.-23.09.2011. P79. Ha r t m a n n , A., S. We i s e , C. Co l m s e e , E. Gr a f a h r e n d -Be l a u , B.H. P89. He d t m a n n , C., S. En d r e s s , A. Ma t r o s & H.-P. Mo c k : Investi- Ju n k e r , C. Kl u k a s , D. Ko s c h ü t z k i , M. La n g e , U. Sc h o l z & F. gation of Arabidopsis HOP/STI1 co-chaperone function Sc h r e i b e r : Systems biology in crop plant metabolism: an and their regulation during stress response. – Instituts- integration and analysis pipeline. – Plant Metabolic En- tag IPK, Gatersleben, 04.-06.10.2011. gineering Gordon Research Seminar, Waterville Valley, P90. He n s e l , G., D.S. Da g h m a , M. Me l z e r & J. Ku m l e h n : Illuminat- NH/USA, 23.-24.07.2011. ing early pollen embryogenesis: Time-lapse livecell im- P80. Ha r t m a n n , A., S. We i s e , C. Co l m s e e , E. Gr a f a h r e n d -Be l a u , aging facilitated by nucleus-specific GFP accumulation. B.H. Ju n k e r , C. Kl u k a s , D. Ko s c h ü t z k i , M. La n g e , U. Sc h o l z & – 11th GABI Status Seminar, Potsdam, 15.-17.03.2011. F. Sc h r e i b e r : Systems biology in crop plant metabolism:

201 Vorträge und Poster/Lectures and Posters

P91. He n s e l , G., M. Gu r u s h i d z e , J. Ri e c h e n , C. Be r g e r , D. Do u c h k o v , P102. Ke l l e r , E.R.J., C.D. Za n k e , T. Ko t l i n s k a , M. Ol a s -So c h a c k a , A. R. Ei c h m a n n , R. Hü c k e l h o v e n , P. Sc h w e i z e r & J. Ku m l e h n : Gen- Re i s & A.M. Ba r a t a : Cryopreservation of young inflores- eration and molecular analysis of transgenic barley and cence bases in bolting garlic for germplasm storage. wheat lines carrying hairpin RNAi constructs directed To serve and conserve. Genebanks exploring ways to towards genes involved in plant-pathogen interactions. improve service to PGR users and effectiveness of PGR – 11th GABI Status Seminar, Potsdam, 15.-17.03.2011. conservation. – EUCARPIA Section Genetic Resources, P92. He n s e l , G., A. Br u c h m ü l l e r , S. Go e d e k e , S. Br o e d e r s , A. Him- European Plant Genetic Resources Conference 2011, m e l b a c h , T. Ru t t e n , J. Ri e c h e n , U. Zi e r o l d , P. Sc h w e i z e r & Wageningen/The Netherlands, 05.-07.04.2011. J. Ku m l e h n : Genetic engineering in barley – methods and P103. Ke l l e r , E.R.J., C. Za n k e , T. Ko t l i ń s k a , M. Ol a s -s o c h a c k a , A. Re i s applications. – Meeting Cost Action Molecular Farming, & A.M. Ba r a t a : Cryopreservation of young inflorescence Ghent/Belgium, 14.-16.09.2011. bases in bolting garlic for germplasm storage. – Instituts- P93. He n s e l , G., D.S. Da g h m a , M. Me l z e r & J. Ku m l e h n : Illuminat- tag IPK, Gatersleben, 04.-06.10.2011. ing early pollen embryogenesis by nucleus-specific GFP P104. Ke r r e n , A., I. Ju s u f i , A. Al e k s a k h i n & F. Sc h r e i b e r : CluMa-GO: accumulation in barley (Hordeum vulgare L.). – Botani- Bring gene ontologies and hierarchical clusterings to- kertagung 2011 “Diversity makes the Difference”,B erlin, gether. – IEEE Symposium on Biological Data Visualization 18.-23.09.2011. (BioVis), Providence, Rhode Island/USA, 23.-24.10.2011. P94. He n s e l , G., A. Hi m m e l b a c h , D. No w a r a , C. Ka s t n e r , D. Do u c h - P105. Kirioukhova, O., D. Kö s z e g i , R. Le m c k e , K. Kr u m m e l , P. Ri z z o , k o v , P. Sc h w e i z e r & J. Ku m l e h n : Development of a plant A. Bu s c h i n g , S. Sk i e b e , H. Bä u m l e i n , W. Gr u i s s e m & A.J. Jo h n - transformation toolbox to study plant-pathogen inter- s t o n : Ups and Downs of the Retinoblastoma pathway actions in cereals. – Botanikertagung 2011 “Diversity control plant reproductive development, differentia- makes the Difference”, Berlin, 18.-23.09.2011. tion and genome integrity. – Institutstag IPK, Gatersle- P95. Ho a n g , P.T., J. Po h l , U. Gr e s c h , F. Ra b e n s t e i n , J. Ve i t s & ben, 04.-06.10.2011. U. Co n r a d : Production and characterization of avian flu P106. Ki s o n , U. & K. Pistrick: Paul Schuster – Pfarrer und Botani- hemagglutinin multimers in transgenic plants. – Insti- ker in Meisdorf. – Ausstellung Patronatskirche Meisdorf, tutstag IPK, Gatersleben, 04.-06.10.2011. Stadt Falkenstein/Harz, 27.08.-25.09.2011. P96. Ho a n g , P.T., J. Po h l , U. Gr e s c h , F. Ra b e n s t e i n , J. Ve i t s & U. Co n - P107. Kl e m m e , S., M. Ma r t i s , A.M. Ba n a e i Mo g h a d d a m , K. Ma y e r , r a d : Production and immunological characterization of T. Wi c k e r , J. Ma c a s , U. Sc h o l z , F. Bu l l , T. Sc h m u t z e r , H. Sim- avian flu hemagglutinin multimers in transgenic plants. k o v a , J. Do l e z e l , N. St e i n , J. Fu c h s & A. Ho u b e n : Evolution of a – Pharma Planta Open Meeting, Brussels/Belgium, selfish chromosome. – 7th Plant Science Student Confer- 25.10.2011. ence, IPB, Halle/S., 14.-17.06.2011. P97. Hü g e , J., H. Po s k a r , C. Kr a c h , M. Fr a n k e , L. Fi c h t m ü l l e r, F. Ke l l - P108. Kl e m m e , S., M. Ma r t i s , A.M. Ba n a e i Mo g h a d d a m , K. Ma y e r , n e r , N. Sc h ä f e r & B.H. Ju n k e r : A pipeline for the derivation T. Wi c k e r , J. Ma c a s , U. Sc h o l z , F. Bu l l , T. Sc h m u t z e r , H. Si m k o v a , of intracellular metabolic fluxes in plant seeds. – Insti- J. Do l e z e l , N. St e i n , J. Fu c h s & A. Ho u b e n : Evolution of a selfish tutstag IPK, Gatersleben, 04.-06.10.2011. chromosome. – 18th International Chromosome Confer- P98. Ju n k e r , B.H., H. Po s k a r , C. Kr a c h , M. Fr a n k e , N. Sc h ä f e r , L. ence, University Place, Manchester/UK, 29.08.-02.09.2011. Fi c h t m ü l l e r, F. Ke l l n e r , H. Ro h n , F. Sc h r e i b e r & J. Hü g e : Meta- P109. Kl e m m e , S., M. Ma r t i s , A.M. Ba n a e i Mo g h a d d a m , F.R. Bl a t t - bolic flux analysis in plant seeds. – Plant MetabolicE ngi- n e r , J. Ma c a s , T. Sc h m u t z e r , U. Sc h o l z , H. Gu n d l a c h , T. Wi- neering Gordon Research Conference, Waterville Valley, c k e r , P. No v a k , P. Ne u m a n n , H. Si m k o v a , J. Do l e z e l , E. Ba u e r , NH/USA, 24.-29.07.2011. G. Ha s e n e y e r , L. Ma, J. Fu c h s , N. St e i n , K.F.X. Ma y e r & A. Ho u - P99. Ka r c e v a , T., S. La n d j e v a & A. Bö r n e r : Effects of wheat Rht- b e n : Structure and evolution of a selfish chromosome. B1b, Rht-B1c and Rht-D1b genes on plant height and – Institutstag IPK, Gatersleben, 04.-06.10.2011. yield potential under the climatic conditions of Bulga- P110. Ko l l e r s , S., M.S. Rö d e r , B. Ro d e m a n n , V. Ko r z u n , O. Ar g i l l i e r, ria. – EWAC-EUCARPIA Cereals Section Conference, Novi C. Pi e t s c h , E. Eb m e y e r , J. Pl i e s k e , P. Jo a q u i m & M. Ga n a l : Asso- Sad/Serbia, 07.-11.11.2011. ciations with Fusarium head blight in a set of elite West- P100. Ka r i m i As h t i y a n i , R., T. Ru t t e n , A.M. Ba n a e i Mo g h a d d a m , ern-European germplasm of hexaploid wheat (Triticum V. Sc h u b e r t , J. Fu c h s & A. Ho u b e n : AtHaspin phospho- aestivum L.). – 11th GABI Status Seminar, Potsdam, 15.- rylates histone H3 at threonine 3 during mitosis and 17.03.2011. contributes to embryonic patterning in Arabidopsis. – P111. Ko l l e r s , S., B. Ro d e m a n n , M.W. Ga n a l , J. Pl i e s k e , O. Ar g i l l i e r, 18th International Chromosome Conference, University M. Sa w k i n s , V. Ko r z u n , E. Eb m e y e r , C. Pi e t s c h & M.S. Rö d e r : Place, Manchester/UK, 29.08.-02.09.2011. Whole genome association mapping of Fusarium head P101. Ka s t n e r , C., A. Hi m m e l b a c h , G. Dö h l e m a n n , P. Sc h w e i z e r , G. He n - blight resistance in European winter wheat. – Instituts- s e l , M. Ga h r t z & J. Ku m l e h n : Establishment of an Agrobac- tag IPK, Gatersleben, 04.-06.10.2011. terium-based transformation method for maize and its P112. Ko o h i De h k o u r d i , M., D. Ri e w e , M. Kh o d a m b a s h i , R.C. Me y e r & use to validate the pathogen-inducible barley GER4c T. Al t m a n n : Reverse genetics for the assessment of essen- promoter. – International Conference “Plant Transfor- tial candidate genes for metabolite content. – 7th Plant mation Technologies II”, Vienna/Austria, 19.-22.02.2011. Science Student Conference, IPB, Halle/S., 14.-17.06.2011.

202 P113. Ko p p o l u , J., A. Boudichevskaia & R. Sc h m i d t : Genetic analysis P126. Le r m o n t o v a , I., O. Ko r o l e v a , T. Ru t t e n , J. Fu c h s , V. Sc h u b e r t , of seed traits in Arabidopsis thaliana under different en- I. Mo r a e s , D. Kö s z e g i & I. Sc h u b e r t : Consequences of CENH3 vironments. – 7th Plant Science Student Conference, IPB, depletion or truncation in Arabidopsis. – Institutstag Halle/S., 14.-17.06.2011. IPK, Gatersleben, 04.-06.10.2011. P114. Ko w a l c z y k , K., A. Bö r n e r , J. Le ś n i o w s k a -No w a k , M. No w a k & P127. Le r m o n t o v a , I., O. Ko r o l e v a , T. Ru t t e n , J. Fu c h s & I. Sc h u b e r t : S. Ok o ń : Analysis of selected quantitative traits in Triti- Consequences of CENH3 depletion or truncation in Ara- cum aestivum/Aegilops squarrosa introgressive lines. – bidopsis. – EMBO Conference Series “Histone Variants EWAC-EUCARPIA Cereals Section Conference, Novi Sad/ and Genome Regulation”, IGBMC, Strasbourg/France, Serbia, 07.-11.11.2011. 12.-14.10.2011. P115. Ko w a l c z y k , K., A. Bö r n e r , M. No w a k , J. Le ś n i o w s k a -No w a k & P128. Li p p m a n n , R., A. Ma t r o s , G. He n s e l , J. Ku m l e h n & H.-P. Mo c k : M. Za p a l s k a : Characterization of quantitative traits of Purification of feeder compounds stimulating pollen Steptoe × Morex barley (Hordeum vulgare L.) popula- embryogenesis. – 11th GABI Status Seminar, Potsdam, tion. – EWAC-EUCARPIA Cereals Section Conference, 15.-17.03.2011. Novi Sad/Serbia, 07.-11.11.2011. P129. Li p p m a n n , R. & H.-P. M o c k : Euphorbia lathyris, a potential crop P116. Kr a c h , C., H. Po s k a r , M. Fr a n k e , N. Sc h ä f e r , L. Fi c h t m ü l l e r, for third generation biofuels – a proteomic approach. – F. Ke l l n e r , J. Hü g e & B.H. Ju n k e r : Quantification of intra- 11th GABI Status Seminar, Potsdam, 15.-17.03.2011. cellular metabolic fluxes in developing legume seeds. P130. Li p p m a n n , R. & H.-P. Mo c k : Euphorbia lathyris, a potential – FORSYS Young Investigator Seminar, Heidelberg, crop for third generation biofuels – a proteomic ap- 14.02.2011. proach. – Meeting COST Action FA0603, Dijon/France, P117. Ku h l m a n n , M., A. Fi n k e & M.F. Me t t e : Genetic analysis of 25.-27.05.2011. RNA-directed transcriptional gene silencing in Arabi- P131. Lo h w a s s e r , U., M.A. Re h m a n Ar i f & A. Bö r n e r : New strategies dopsis thaliana. – SFB 648 International Meeting “Com- for discovery loci determining pre-harvest sprouting munication in Plants and their Responses to the Envi- and dormancy in wheat and barley. – Institutstag IPK, ronment”, Halle/S., 19.-21.05.2011. Gatersleben, 04.-06.10.2011. P118. Ku h l m a n n , M., A. Fi n k e & M.F. Me t t e : Genetic analysis of P132. Lo h w a s s e r , U., M.A. Re h m a n Ar i f & A. Bö r n e r : Comparative RNA-directed transcriptional gene silencing in Ara- mapping of loci determining pre-harvest sprouting and bidopsis thaliana. – Botanikertagung 2011 “Diversity dormancy in wheat and barley. – EWAC-EUCARPIA Cere- makes the Difference”, Berlin, 18.-23.09.2011. als Section Conference, Novi Sad/Serbia, 07.-11.11.2011. P119. La g i n h a , A.M., R.F.H. Gi e h l , L. Yu a n , D. Re n t s c h & N. v o n Wi- P133. Lü p k e n , T., A. Ha b e k u s s , D. Pe r o v i c , I. Kr ä m e r , U. Hä h n e l , B. r é n : Physiological characterization of the ammonium St e u e r n a g e l , R. Ar i y a d a s a , R. Zh o u , N. St e i n , M. Ma r t i s , K.F.X. transporter AMT2;1 in Arabidopsis thaliana. – Botani- Ma y e r & F. Or d o n : High resolution mapping of the BaM- kertagung 2011 “Diversity makes the Difference”,B erlin, MV/BaYMV resistance gene rym11 by employing syn- 18.-23.09.2011. teny and next generation sequencing data for marker P120. La n g e , M., J. Ch e n , D. Bo r c k , C. Co l m s e e , K. Hi p p e , B. Ko r m e i e r , saturation. – VIIIth International Working Group of Plant S. We i s e & U. Sc h o l z : Building information retrieval por- Viruses with Fungal Vectors, Louvain-la-Neuve/Belgium, tals using the lailaps search engine. – 6th International 06.-08.07.2011. Symposium on Health Informatics and Bioinformatics P134. Ma, L., E. Ka p u s i , C.H. Te o , G. He n s e l , J. Ku m l e h n , I. Sc h u b e r t , M.F. (HIBIT 2011), Izmir/Turkey, 02.-05.05.2011. Me t t e & A. Ho u b e n : Telomere-mediated truncation of barley P121. La n g e , M., D. Sc h ü l e r , C. Co l m s e e , S. Fl e m m i n g , D. Ar e n d & chromosomes. – 18th International Chromosome Confer- U. Sc h o l z : LIMSOPHY – How to implement a LIMS at IPK. ence, University Place, Manchester/UK, 29.08.-02.09.2011. – Institutstag IPK, Gatersleben, 04.-06.10.2011. P135. Ma c a s , J., S. Kl e m m e , P. No v á k , P. Ne u m a n n , A.M. Ba n a e i P122. La p i n , D., R.C. Me y e r & G. v a n d e n Ac k e r v e k e n : Genetic map- Mo g h a d d a m , N. St e i n , H. Ši m k o v á , J. Do l e ž e l & A. Ho u b e n : ping of broad resistance to downy mildew in Arabidopsis Comparative analysis of repetitive DNA based on bio- C24. – International Meeting “Communication in Plants informatic analysis of next generation sequencing data and their Responses to the Environment”, Martin-Luther- reveals differences in repeat composition between A Universität Halle-Wittenberg, Halle/S., 19.-22.05.2011. and B chromosomes of rye. – EU COST Action Workshop P123. Le Ho n g , D., A.J. Jo h n s t o n , O. Kirioukhova, A. Cz i h a l , T. Ru t t e n , “Statistical challenges on 1000 euro genome sequences R. Iv a n o v & H. Bä u m l e i n : Reproductive functions of EFFEC- in plants”, Toulouse/France, 14.-15.04.2011. TOR OF TRANSCRIPTION. – Institutstag IPK, Gatersleben, P136. Ma r c o n i , G., G. Ga l l a , J. Co n n e r , L. Ra g g i , G. Ba r c a c c i a , 04.-06.10.2011. P. Oz i a s -Ak i n s , T.F. Sh a r b e l , M. Fa l c i n e l l i & E. Al b e r t i n i : Cross- P124. Le, T.L., M. Ar l t & R. Sc h m i d t : Post-transcriptional trans- species characterization of APOSTART for unveiling its gene silencing in Arabidopsis thaliana – analysis of nat- role in apomixis. – Plant and Animal Genome XIX. Con- ural variation. – 7th Plant Science Student Conference, ference, San Diego/USA, 15.-19.01.2011. IPB, Halle/S., 14.-17.06.2011. P125. Le, T.L. & R. Sc h m i d t : Post-transcriptional transgene silencing in Arabidopsis thaliana – analysis of natural variation. – Institutstag IPK, Gatersleben, 04.-06.10.2011.

203 Vorträge und Poster/Lectures and Posters

P137. Ma r z e c , M., I. Sz a r e j k o , D.S. Da g h m a , T. Ru t t e n , K. Ho f f i e , M. P148. Mi d d l e t o n , C., B. Kilian, N. St e i n & T. Wi c k e r : 454 snapshot Wi e s n e r & M. Me l z e r : Structural and molecular charac- provides insights into the composition and divergence terization of barley root epidermal cells for identifica- of several Triticeae members. – Plant and Animal Ge- tion of new genes involved in root hair development nome XIX. Conference, San Diego/USA, 15.-19.01.2011. in Hordeum vulgare. – Institutstag IPK, Gatersleben, 04.- P149. Mö n k e , G., H. Zi e r m a n n , J. Ke i l w a g e n & U. Co n r a d : Seed- 06.10.2011. specific transcription factors ABI3 and FUS3 show high P138. Ma r z i n , S., A. Ha n e m a n n , S. Sh a r m a , G. Sc h w e i z e r & M.S. Rö d e r : binding motif specificity. – Institutstag IPK, Gatersle- Pectin esterase inhibitor gene: A possible candidate for ben, 04.-06.10.2011. the resistance gene Rrs2 against Rhynchosporium secalis P150. Mo s t e k , A., S. Lü t h j e , A. Bö r n e r & A. We i d n e r : Comparison of in barley. – Institutstag IPK, Gatersleben, 04.-06.10.2011. different methods of isolation and solubilization of mem- P139. Ma s c h e r , M., U. Sc h o l z & S. Fr i e d e l : Chargaff’s second rule brane proteins isolated from roots of a salinity tolerant in plant genomes. – 12th International Conference on barley line germinating under salinity stress. – COST Ac- Systems Biology (ICSB2011), Heidelberg/Mannheim, tion FA0901 WG1 Meeting “Ecology of Halophytes and 28.08.-01.09.2011. Saline Habitats”, Torun/Poland, 11.-12.05.2011. P140. Ma s c h e r , M., C. Co l m s e e , A. Ha r t m a n n , T. Cz a u d e r n a , P151. Mu e h l b a u e r , G., T.J. Cl o s e , A. Gr a n e r , P. La n g r i d g e , C. Li, U. Sc h l ü t e r , F. Sc h r e i b e r & U. Sc h o l z : Co-expression net- M. Ma t s u m o t o , K. Sa t o , A. Sc h u l m a n , R. Wa u g h , R. Wi s e , G. work analysis identifies hub genes in maize. – Instituts- Zh a n g & N. St e i n : A sequence of the barley genome in tag IPK, Gatersleben, 04.-06.10.2011. 2011. – Plant and Animal Genome XIX. Conference, San P141. Ma t t h i e s , I.E., M. Ma l o s e t t i , S. We i s e , F. v a n Ee u w i j k & Diego/USA, 15.-19.01.2011. M.S. Rö d e r : GENOBAR: Genome-wide association map- P152. Mu r a y a , M.M., T. Sc h m u t z e r , U. Sc h o l z & T. Al t m a n n : Plant- ping for kernel and malting quality traits using histori- KBBE Cornfed: Integration of advanced mapping and cal European barley reports. – 11th GABI Status Seminar, phenotyping methods to identify key alleles for build- Potsdam, 15.-17.03.2011. ing European maize ideotypes – genetic and pheno- P142. Ma y e r , K.F.X., M. Ma r t i s , N. Poursarebani , P.E. He d l e y , H. Si m k - typic analysis of biomass production and water use o v a , H. Liu, J.A. Mo r r i s , B. St e u e r n a g e l , S. Ta u d i e n , S. Ro e s s n d e r , efficiency (WUE). – 11th GABI Status Seminar, Potsdam, H. Gu n d l a c h , T. Nu s s b a u m e r , P. Su c h a n k o v a , M. Ku b a l a k o v a , 15.-17.03.2011. F. Mu r a t , M. Fe l d e r , A. Gr a n e r , J. Sa l s e , T. En d o , H. Sa k a i , P153. Na g e l , M., M.A. Re h m a n Ar i f , H. Ts c h i e r s c h , H. Ro l l e t s c h e k , T. Ta n a k a , T. It o h , K. Sa t o , M. Pl a t z e r , M. Ma t s u m o t o , L. Bo r i s j u k , I. Kr a n n e r & A. Bö r n e r : Genetic variation of U. Sc h o l z , J. Do l e z e l , R. Wa u g h & N. St e i n : GenomeZipper: seed deterioration in wheat. – 10th International Confer- A synteny driven approach to unlock the barley genome. ence of the International Society for Seed Science (ISSS), – 11th GABI Status Seminar, Potsdam, 15.-17.03.2011. Costa do Sauípe, Bahia/Brazil, 10.-15.04.2011. P143. Me i t z e l , T., R. Ra d c h u k , H. We b e r & W. We s c h k e : Seed specific P154. Na g e l , M., M.A. Re h m a n Ar i f , M. Al l a m , I.O. Da n i e l , M. Gä b l e r , modulation of the trehalose-6-phosphate level in pea. – R.K. Pa s a m , B. Kilian, N. St e i n & A. Bö r n e r : Viability of seed Institutstag IPK, Gatersleben, 04.-06.10.2011. collections in genebanks – crucial aspects of seed lon- P144. Me l z e r , B., T. Gu s e , C. Ka s t n e r , C. Be r g e r , E. Ka p u s i , G. He n s e l & gevity. – Institutstag IPK, Gatersleben, 04.-06.10.2011. J. Ku m l e h n : Agrobacterium-mediated transformation of P155. Na g e l , M., I.O. Da n i e l , M. Gä b l e r , R.K. Pa s a m , M.A. Re h m a n Brachypodium distachyon using shoot segments. – Ar i f , B. Kilian, N. St e i n & A. Bö r n e r : Seed longevity in cereal 1st European Brachypodium Workshop, Paris/France, collections – variation and gene identification. –E WAC- 19.-21.10.2011. EUCARPIA Cereals Section Conference, Novi Sad/Serbia, P145. Me y e r , R.C., H. Sc h ö n , A. Sc h l e r e t h , T. St r u c k m e y e r , I. Fi s c h e r , 07.-11.11.2011. W.-R. Sc h e i b l e , H. Wi t u c k a -Wa l l , M. v o n Ko r f f , M. Mü l l e r, O. P156. Ne h r l i c h , S., E. Wi l l n e r & K.J. De h m e r : Characterization and Th i m m , M. St i t t & T. Al t m a n n : FUNCIN: Using natural diver- evaluation of Genebank accessions as a pre-selection sity of Arabidopsis to identify lead genes and metabolic instrument for plant breeding objectives and strate- markers to improve nitrogen utilization in crops. – 11th gies. – EUCARPIA Section Fodder Crops and Amenity GABI Status Seminar, Potsdam, 15.-17.03.2011. Grasses, Dublin/Ireland, 05.-08.09.2011. P146. Me y e r , R.C., K. We i g e l t , M. Se y f a r t h , F.A. Ar a n a -Ce b a l l o s , P157. Ne h r l i c h , S., S. We i s e , S. Wi t t e r , E. Wi l l n e r, U. Sc h o l z & H. Wi t u c k a -Wa l l , M. Me l z e r & T. Al t m a n n : Biomass and leaf K.J. De h m e r : DiSTo2 – a diversity studies toolkit for pheno- area in the study of heterosis in Arabidopsis thaliana. – typic, genotypic and passport data of genebank acces- 2nd International Plant Phenotyping Symposium, For- sions. – Institutstag IPK, Gatersleben, 04.-06.10.2011. schungszentrum Jülich, 05.-07.09.2011. P158. No w a r a , D., W. Ch e n , G. He n s e l , J. Ku m l e h n & P. Sc h w e i z e r : P147. Me y e r , R.C., F.A. Ar a n a -Ce b a l l o s , C. Gr y c z k a , H. Je o n , HIGS: Host-induced gene silencing in phytopathogenic M. Ko o h i , M.M. Mu r a y a , M. Pf e i f e r , D. Ri e w e , M.G. Ro s s o , fungi. – Plant and Animal Genome XIX. Conference, San M. Se y f a r t h , C. We b e r , K. We i g e l t -Fi s c h e r & T. Al t m a n n : Phe- Diego/USA, 15.-19.01.2011. notypic, genetic and metabolic analyses of vegetative P159. No w a r a , D., G. He n s e l , A. Ga y , D. D o u c h k o v , P. S c h w e i z e r & J. Ku m - biomass accumulation in Arabidopsis thaliana and Zea l e h n : Host-induced gene silencing in fungal pathogens of mays. – Institutstag IPK, Gatersleben, 04.-06.10.2011. cereals. – International Conference “Plant Transformation Technologies II”, Vienna/Austria, 19.-22.02.2011.

204 P160. No w a r a , D., G. He n s e l , A. Ga y , D. Do u c h k o v , P. Sc h w e i z e r & P172. Po s k a r , C.H., C. Kr a c h , M. Fr a n k e , N. Sc h ä f e r , L. Fi c h t m ü l l e r, J. Ku m l e h n : Host-induced Gene Silencing (HIGS) in fungal F. Ke l l n e r , J. Hü g e & B.H. Ju n k e r : A pipeline for the deriva- pathogens of cereals. – Gentechnik-Workshop der Ge- tion of intracellular metabolic fluxes in developing le- sellschaft für Pflanzenbiotechnologie, Quedlinburg und gume seeds. – Society for Experimental Biology Annual Üplingen, 22.-23.08.2011. Main Meeting, Glasgow/UK, 01.-04.07.2011. P161. No w a r a , D., A. Ga y , G. He n s e l , J. Ku m l e h n & P. Sc h w e i z e r : Host- P173. Poursarebani , N., R. Ar i y a d a s a , D. Sc h u l t e , R. Zh o u , B. St e u e r - induced gene silencing in fungal plant pathogens. – Ins- n a g e l , M. Ma r t i s , K.F.X. Ma y e r , U. Sc h o l z , A. Gr a n e r & N. St e i n : titutstag IPK, Gatersleben, 04.-06.10.2011. Potential of grass genome synteny for genetic anchoring P162. Nü r k , N.M. & F.R. Bl a t t n e r : Biogeography of the genus Hy- of the physical map of barley (Hordeum vulgare L.) – A pericum. – Institutstag IPK, Gatersleben, 04.-06.10.2011. case study on chromosome 2H. – Plant and Animal Ge- P163. Or d o n , F., J. Ro d e , T. La n g e , B. Kilian, A. Gr a n e r , R. Pa s a m , nome XIX. Conference, San Diego/USA, 15.-19.01.2011. I. Ma t t h i e s , M.S. Rö d e r , S. We i s e , U. Sc h o l z , F. Ur b a n , J. Ah l - P174. Ra d c h u k , V., D. We i e r , R. Ra d c h u k , H. We b e r & W. We s c h k e : Ex- e m e y e r , W. Fr i e d t , I. Ga w e n k a , K. Sc h m i d , J. Gr o e r , V. Ko r z u n , pression of distinct VPE genes coincides with the disinte- J. We y e n , J. Fö r s t e r , R. He m k e r , F. We s p e l , E. La u b a c h , E. Kn o p f , gration processes in developing barley grains. – 1st Plant H. Bl ü m e l , H. Ja i s e r , P. Gr e i f , L. Cs e l e n y i & U. St e p h a n : GABI Protease Conference, Hemavan/Sweden, 11.-14.04.2011. GENOBAR: A genome-wide approach to associate genet- P175. Ra j e s h , K., V. Harshavadhan , H. Ro l l e t s c h e k , K.-P. G ö t z , C. Se i l e r , ic diversity with agronomically important traits in barley. M. St r i c k e r t , V. Ko r z u n , B. Us a d e l , R. Su l p i c e , M. St i t t , U. Wo b u s – 11th GABI Status Seminar, Potsdam, 15.-17.03.2011. & N. Sreenivasulu : Importance of senescence induced re- P164. Os i p o v a , S.V., A.V. Pe r m y a k o v , M.D. Pe r m y a k o v a , T.A. Ps h e n i c h - mobilization in determining the crop yield in elite lines of n i k o v a & A. Bö r n e r : Genetic variability of detoxification barley under terminal drought. – 11th GABI Status Semi- enzymes activity in leaves of inter-varietal substitution nar, Potsdam, 15.-17.03.2011. lines of bread wheat with different tolerance to water P176. Re h m a n Ar i f , M.A., M. Na g e l , K. Ne u m a n n , U. Lo h w a s s e r , B. Ko- deficit. – EWAC-EUCARPIA Cereals Section Conference, b i l j s k i & A. Bö r n e r : Genome-wide association mapping of Novi Sad/Serbia, 07.-11.11.2011. longevity, dormancy and pre-harvest sprouting in bread P165. Pa s a m , R., R. Sh a r m a , B. Kilian & A. Gr a n e r : Whole genome wheat (Triticum aestivum L.). – EWAC-EUCARPIA Cereals association mapping in barley: effects of marker densi- Section Conference, Novi Sad/Serbia, 07.-11.11.2011. ty on QTL discovery and significance. – Institutstag IPK, P177. Ro h n , H., C. Kl u k a s & F. Sc h r e i b e r : Visual analytics of mul- Gatersleben, 04.-06.10.2011. timodal biological data. – International Conference P166. Pe l l i n o , M., D. Ho j s g a a r d , H. Vo g e l , T. Sc h m u t z e r , J.M. Co r - on Information Visualization Theory and Applications r a l , E. Hö r a n d l & T.F. Sh a r b e l : Comparative transcriptomal (IVAPP2011), Vitamoura/Portugal, 05.-07.03.2011. profiling live microdissected ovules from sexual and P178. Ro h n , H. & F. Sc h r e i b e r : Combining different types of bio- apomictic Ranunculus. – Institutstag IPK, Gatersleben, logical data. – International Symposium on Integrative 04.-06.10.2011. Bioinformatics 2011 (IB2011), Wageningen/The Nether- P167. Pe r o v i c , D., J. Kö n i g , D. Ko p a h n k e , B.J. St e f f e n s o n , J. Fö r s t e r , B. lands, 21.-23.03.2011. Kilian, J. Pl i e s k e & F. Or d o n : MBR1012 × Scarlett: A new DH P179. Ró i s , A.S., D. Espírito-Sa n t o , T.F. Sh a r b e l & A.D. Ca p e r t a : Cy- population for genetic dissection of resistance to dif- togenetic diversity and ploidy variation in diploid and ferent pathogens in barley. – EWAC-EUCARPIA Cereals polyploid Limonium species. – 13th Congress of the Euro- Section Conference, Novi Sad/Serbia, 07.-11.11.2011. pean Society for Evolutionary Biology (ESEB2011), P168. Pe u k e r t , M., A. Ma t r o s & H.-P. Mo c k : MALDI-MS imaging – Tübingen, 20.-25.08.2011. a tool for characterization of tissue specific distribution P180. Ro l l e t s c h e k , H., G. Me l k u s , E. Gr a f a h r e n d -Be l a u , J. Fu c h s , pattern of small molecules during barley grain devel- N. He i n z e l & L. Bo r i s j u k : Combined non-invasive imaging opment. – Botanikertagung 2011 “Diversity makes the and modeling approaches reveal metabolic compart- Difference”, Berlin, 18.-23.09.2011. mentation in the barley endosperm. – 12th International P169. Ph a n , H.P., B.T. Le, D. Fl o s s , J. Sc h e l l e r & U. Co n r a d : Purifica- Conference on Systems Biology (ICSB2011), Heidelberg/ tion and characterization of ELPylated antigens of the Mannheim, 28.08.-01.09.2011. H5N1 avian flu virus inNicotiana tabacum. – 7th Plant Sci- P181. Ro l l e t s c h e k , H., G. Me l k u s , E. Gr a f a h r e n d -Be l a u , J. Fu c h s , N. ence Student Conference, IPB, Halle/S., 14.-17.06.2011. He i n z e l , F. Sc h r e i b e r , P.M. Ja k o b & L. Bo r i s j u k : Combined non- P170. Ph a n , H.P., B.T. Le, D. Fl o s s , J. Sc h e l l e r & U. Co n r a d : Purifica- invasive imaging and modelling approaches reveal meta- tion and characterization of ELPylated antigens of the bolic compartmentation in the cereal endosperm. – 71st H5N1 avian flu virus in Nicotiana tabacum. – COST Ac- Harden Conference on Metabolic Pathway Analysis, Uni- tion, Molecular Farming: Plants as a Production Platform versity of Chester, Cheshire, England/UK, 19.-23.09.2011. for High Value Proteins, Napoli/Italy, 06.-08.10.2011. P182. Ro l l e t s c h e k , H., N. He i n z e l , H. Ts c h i e r s c h , J. Fu c h s , T. Al t m a n n & L. P171. Pistrick, K., M. Ak h a l k a t s i & J. Ek h v a i a : Plant genetic re- Bo r i s j u k : Modelling of growth and storage metabolism in sources of higher altitudes in southeastern Georgia crop seeds. – Institutstag IPK, Gatersleben, 04.-06.10.2011. (Samcche-Džavacheti and Atčara). – Institutstag IPK, P183. Ru b t s o v a , M., K. Ke m p e & M. Gi l s : A split gene approach for Gatersleben, 04.-06.10.2011. hybrid wheat production. – Institutstag IPK, Gatersle- ben, 04.-06.10.2011.

205 Vorträge und Poster/Lectures and Posters

P184. Ru t t e n , T., D. Kö s z e g i , A.J. Jo h n s t o n , A. Cz i h a l , J. Ku m l e h n , man Workshop on Tibetan Plateau Research, Hamburg, S. Wu e s t , O. Kirioukhova, J. Gh e y s e l i n c k , L. Al t s c h m i e d , 03.-05.03.2011. G. Mö n k e , M. Me l z e r & H. Bä u m l e i n : Studies on the egg-cell P196. Seidensticker , T., A. Bö r n e r & T. Sc h n u r b u s c h : Towards ge- specific transcription factors RKD1 & RKD2. – 9th Interna- netic elucidation of gene(s) regulating inflorescence tional Botanical Microscopy Meeting, Wageningen/The branching in wheat and barley. – 11th GABI Status Semi- Netherlands, 16.-21.04.2011. nar, Potsdam, 15.-17.03.2011. P185. Sainis, J.K., R. Ag a r w a l , K.H. Sü s s & M. Me l z e r : Immune elec- P197. Seidensticker , T., A. Bö r n e r & T. Sc h n u r b u s c h : Towards the tron microscopy suggests that soluble enzymes form molecular elucidation of ‘Miracle Wheat’ - wheats with a thin layer on the surface of thylakoid membranes. – branched inflorescences. – 21st Workshop of the Inter- Gordon Research Conference on Photosynthesis, Da- national Triticeae Mapping Initiative (ITMI), Mexico City/ vidson/USA, 12.-17.06.2011. Mexico, 05.-09.09.2011. P186. Sa l e m , K.F.M., M.S. Rö d e r & A. Bö r n e r : Evaluation of ge- P198. Seidensticker , T., L. Ro s s i n i , U. Lu n d q v i s t & T. Sc h n u r b u s c h : netic diversity among Egyptian bread wheat (Triticum Compositum barley – towards genetic elucidation of aestivum L.) varieties during the period 1947-2004 us- gene(s) regulating inflorescence branching. – 21st Work- ing microsatellites. – EWAC-EUCARPIA Cereals Section shop of the International Triticeae Mapping Initiative Conference, Novi Sad/Serbia, 07.-11.11.2011. (ITMI), Mexico City/Mexico, 05.-09.09.2011. P187. Sa n e i , M., R. Pickering, K. Ku m k e , S. Na s u d a & A. Ho u b e n : Loss P199. Seidensticker , T., A. Bö r n e r & T. Sc h n u r b u s c h : Towards the of CENH3 from centromeres preceds uniparental chro- molecular elucidation of ‘Miracle Wheat’ – wheats with mosome elimination in interspecific barley hybrids. – branched inflorescences. – Annual Conference of the Ger- 18th International Chromosome Conference, University man Genetics Society (GfG), Würzburg, 14.-16.09.2011. Place, Manchester/UK, 29.08.-02.09.2011. P200. Seidensticker , T., L. Ro s s i n i , A. Bö r n e r , U. Lu n d q v i s t & P188. Sa n e i , M., R. Pickering, K. Ku m k e , S. Na s u d a & A. Ho u b e n : Loss T. Sc h n u r b u s c h : Towards genetic elucidation of gene(s) of CENH3 from centromeres precedes uniparental chro- regulating inflorescence branching in wheat and bar- mosome elimination in interspecific barley hybrids. – ley. – Institutstag IPK, Gatersleben, 04.-06.10.2011. Institutstag IPK, Gatersleben, 04.-06.10.2011. P201. Se i f e r t , M., A.M. Ba n a e i Mo g h a d d a m , A. Go h r , M. St r i c k e r t & I. P189. Sa u r , A., I.P. Pa t h u r i , G. He n s e l , D. Za j i c , R. Pr ö l s , R. Hü- Gr o s s e : Parsimonious higher-order Hidden Markov Mod- c k e l h o v e n , J. Ku m l e h n & L.M. Vo l l : Suppression of starch els for enhanced comparative genomics. – Instituts- biosynthesis compromises non-host resistance in bar- tag IPK, Gatersleben, 04.-06.10.2011. ley. – SFB 648 International Meeting “Communication P202. Se i l e r , C., V. Harshavadhan , R. Ka l l a d a n , G. He n z e l , J. Ku m l e h n in Plants and their Responses to the Environment”, & N. Sreenivasulu : Fine tuned modulation of receptor ki- Halle/S., 19.-21.05.2011. nase RPK1 in source and sink tissues lead to altered ABA P190. Sc h a l l a u , K., S. Bu n d t z e n , T. Liiving, S. We b e r , S.M. Ba k e r , V. Pe t e r homeostasis and improved drought tolerance in barley. & B.H. Ju n k e r : Metabolic engineering based on predictions – Institutstag IPK, Gatersleben, 04.-06.10.2011. from computational models of starch synthesis in plant P203. Se y f a r t h , M., K. We i g e l t , D. Ri e w e , R.C. Me y e r & T. Al t m a n n : seeds. – Institutstag IPK, Gatersleben, 04.-06.10.2011. Identification and characterization of functional diver- P191. Sc h l e r e t h , A., H. Ts c h o e p , W.R. Sc h e i b l e , R.C. Me y e r , M. Mü l - sity of regulatory proteins controlling plant growth. – l e r , H. Sc h ö n , T. Al t m a n n & M. St i t t : GABI-ENERGY: Biomass 7th Plant Science Student Conference, IPB, Halle/S., 14.- production in maize – genomics guided breeding of 17.06.2011. energy maize and a systems-oriented analysis; geno- P204. Se y f a r t h , M., K. We i g e l t , R.C. Me y e r & T. Al t m a n n : Identifi- typing of inbred line panel and phenotyping of inbreds cation and characterization of functional diversity of and test crosses under glass house conditions. – 11th regulatory proteins controlling plant growth. – Botani- GABI Status Seminar, Potsdam, 15.-17.03.2011. kertagung 2011 “Diversity makes the Difference”,B erlin, P192. Sc h r e i b e r , F. & T. Cz a u d e r n a : SBGN-ED – working with the 18.-23.09.2011. Systems Biology Graphical Notation. – 12th Interna- P205. Sh i , R.: Senescence-induced iron remobilization in tional Conference on Systems Biology (ICSB2011), source leaves as a prerequisite for iron retranslocation Heidelberg/Mannheim, 28.08.-01.09.2011. in barley plants. – Botanikertagung 2011 “Diversity P193. Sc h u b e r t , I., V. Sc h u b e r t & J. Fu c h s : DNA double-strand makes the Difference”, Berlin, 18.-23.09.2011. break recombination repair in plants – is ‘Break-Induced P206. Sp r i n g m a n n , C., S. Hö r d i n g , T. Me c h a n i k , F. Ba k o s & J. Ku m l e h n : Replication’ a real phenomenon? – Institutstag IPK, Development of commercially applicable methods to Gatersleben, 04.-06.10.2011. generate doubled haploid lines in cereals. – 11th GABI P194. Sedzielewska , K.A., D. Kl e m a n n , J. Fu c h s , N.M. Nü r k , K. Ve t t e r , Status Seminar, Potsdam, 15.-17.03.2011. R. Wa t z k e & G. Ku n z e : A molecular approach to character- P207. St e u e r n a g e l , B., T. Wi c k e r , H. Gu n d l a c h , K.F.X. Ma y e r , N. St e i n ize Glomus AMykor isolate. – 26th Fungal Genetic Con- & U. Sc h o l z : Assembly validation and scaffolding using ference, Boston/USA, 15.-20.03.2011. transposable element information. – Plant and Animal Ge- P195. Se e b e r , E., G. Wi n t e r f e l d , T.F. Sh a r b e l , W. Du r k a , I. He n s e n , nome XIX. Conference, San Diego/USA, 15.-19.01.2011. J. Liu, H. Mi e h e & K. We s c h e : A study on generative repro- P208. St e u e r n a g e l , B., S. Ta u d i e n , H. Gu n d l a c h , M. Gr o t h , M. Fe l d e r , duction, clonal growth and polyploidy. – 7th Sino-Ger- T. Nu s s b a u m e r , M. Pl a t z e r , K.F.X. Ma y e r , U. Sc h o l z & N. St e i n :

206 WGS sequencing of barley: A current status. – 11th GABI natural diversity exploitation in the Arabidopsis popu- Status Seminar, Potsdam, 15.-17.03.2011. lation; Identification and characterization of genes P209. Sz i r a , F., A. Bö r n e r , K. Ne u m a n n , K. Za y n a l i Ne z h a d , G. Ga l i b a controlling vegetative biomass accumulation in Arabi- & A.F. Ba l i n t : EST-based markers associated with QTLs for dopsis thaliana. – 11th GABI Status Seminar, Potsdam, drought tolerance in barley (Hordeum vulgare) could be 15.-17.03.2011. used for marker-assisted selection. – Agrisafe Final Con- P220. We i g e l t , K., R.C. Me y e r , D. Ri e w e , I. Mü c k e & T. Al t m a n n : Use ference “Climate change: challenge for training of applied of high-throughput phenotyping platforms to identify plant scientists”, Budapest/Hungary, 21.-23.03.2011. and characterize genes controlling vegetative biomass P210. Ta v a k o l , E., G. Ve r d e r i o , T. Fu s c a , S. Ci a n n a m e a , T.J. Cl o s e , accumulation in Arabidopsis thaliana. – 2nd International A. Dr u k a , R. Wa u g h , K.F.X. Ma y e r , M. Ma r t i s , R. Ar i y a d a s a , Plant Phenotyping Symposium, Forschungszentrum D. Sc h u l t e , R. Zh o u , N. St e i n , G. Mu e h l b a u e r & L. Ro s s i n i : Jülich, 05.-07.09.2011. Fine mapping and steps towards map-based cloning P221. Wi c k e r , T., K.F.X. Ma y e r , H. Gu n d l a c h , B. St e u e r n a g e l , of Cul4, a Mendelian locus required for tillering in bar- U. Sc h o l z , A. Gr a n e r , J. Do l e z e l , H. Si m k o v a , F. Ch o u l e t , ley. – Plant and Animal Genome XIX. Conference, San C. Fe u i l l e t , T. Fa h i m a , H. Bu d a k , B. Ke l l e r & N. St e i n : Report Diego/USA, 15.-19.01.2011. on low-pass sequencing of flow-sorted wheat group P211. Te o , C.H., L. Ma, E. Ka p u s i , G. He n s e l , J. Ku m l e h n , I. Sc h u b e r t , 1 chromosome arms. – Plant and Animal Genome XIX. A. Ho u b e n & M.F. Me t t e : Construction of engineered Conference, San Diego/USA, 15.-19.01.2011. minichromosomes in Hordeum vulgare and Arabidopsis P222. Wi c k e r , T., K.F.X. Ma y e r , H. Gu n d l a c h , B. St e u e r n a g e l , thaliana. – 18th International Chromosome Conference, U. Sc h o l z , A. Gr a n e r , J. Do l e z e l , H. Si m k o v a , F. Ch o u l e t , University Place, Manchester/UK, 29.08.-02.09.2011. C. Fe u i l l e t , T. Fa h i m a , H. Bu d a k , B. Ke l l e r & N. St e i n : Too P212. Te o , C.H., L. Ma, E. Ka p u s i , G. He n s e l , J. Ku m l e h n , I. Sc h u b e r t , many genes and pseudogenes – runaway amplifica- A. Ho u b e n & M.F. Me t t e : Towards construction of engi- tion of genic sequences in barley and wheat? – Plant neered plant minichromosomes. – Institutstag IPK, and Animal Genome XIX. Conference, San Diego/USA, Gatersleben, 04.-06.10.2011. 15.-19.01.2011. P213. Th i e l , T. & T.F. Sh a r b e l : Scrutinizing gene copy number varia- P223. Wi l l n e r, E., S. Ne h r l i c h & K.J. De h m e r : A European Forage tion (CNV) as a factor for apomixis expression in Boechera. Collection (EFC) as basis for AEGIS and the planned – 9th IMPRS Symposium, Dornburg, 15.02.2011. Euro Genebank – Which final requirements are needed? P214. Ti s o n , J.M., A. Pe t e r s o n , D. Ha r p k e & L. Pe r u z z i : Phylogenetic – EUCARPIA Section Genetic Resources, European Plant analysis shed new light into taxonomy and reticulate evo- Genetic Resources Conference 2011, Wageningen/The lution of the critical Mediterranean Gagea sect. Didymo- Netherlands, 05.-07.04.2011. bulbos (Liliaceae). – BioSystematics, Berlin, 21.-27.02.2011. P224. Wi t t e r , S. & K.J. De h m e r : Establishment of heterotic pools P215. Ts c h i e r s c h , H., L. Bo r i s j u k & H. Ro l l e t s c h e k : Modelling seed in Lolium perenne L. as basis for biomass hybrid breed- photosynthesis by IMAGING-PAM reveals gradient dis- ing. – 7th Plant Science Student Conference, IPB, Halle/S., tribution and adjustments to low light supply. – Interna- 14.-17.06.2011. tional Conference on Tetrapyrole Photoreceptors of Pho- P225. Wi t t e r , S., S. We i s e , J. Fö r s t e r & K.J. De h m e r : Heterotic pools tosynthetics Organisms (ICTPPO), Berlin, 24.-28.07.2011. in perennial ryegrass as basis for biomass hybrid breed- P216. v a n Ie r s e l , M.P., S.E. Bo y d , F. Be r g m a n n , S. Mo o d i e , F. Sc h r e i b e r , ing. – Botanikertagung 2011 “Diversity makes the Dif- T. Cz a u d e r n a , E. De m i r , N. Le No v è r e , A. So r o k i n , H. Mi, ference”, Berlin, 18.-23.09.2011. A. Lu n a , U. Do g r u s o z , Y. Ma t s u o k a , A. Fu n a h a s h i , H. Ki t a n o , P226. Wo r c h , S., N. Sreenivasulu , K. Ra j e s h , L. Ku n z e & M.S. Rö d e r : M.I. Al a d j e m , M.L. Bl i n o v & A.C. Vi l l é g e r : LibSBGN: elec- Candidate genes for grain yield and quality under ter- tronic exchange of SBGN maps. – HARMONY 2011: The minal drought stress in barley. – 11th GABI Status Semi- Hackathon on Resources for Modeling in Biology, New nar, Potsdam, 15.-17.03.2011. York/USA, 18.-22.04.2011. P227. Ye, F. & N. v o n Wi r é n : Iron as a determinant of virulence P217. v a n Ie r s e l , M.P., S.E. Bo y d , F. Be r g m a n n , S. Mo o d i e , F. Sc h r e i b e r , and resistance in the Colletotrichum graminicola – maize T. Cz a u d e r n a , E. De m i r , N. Le No v è r e , A. So r o k i n , H. Mi, interaction. – Botanikertagung 2011 “Diversity makes A. Lu n a , U. Do g r u s o z , Y. Ma t s u o k a , A. Fu n a h a s h i , H. Ki t a n o , the Difference”, Berlin, 18.-23.09.2011. M.I. Al a d j e m , M.L. Bl i n o v & A.C. Vi l l é g e r : LibSBGN: elec- P228. Zh o u , R., G. Ha s e n e y e r , T. Sc h m u t z e r , M. Se i d e l , C.C. Sc h ö n , tronic processing of SBGN maps. – COMBINE 2011, E. Ba u e r , U. Sc h o l z , K.F.X. Ma y e r & N. St e i n : A high densi- Heidelberg, 03.-07.09.2011. ty transcript map of rye (Secale cereale L.) comprising P218. Vu, G.T.H., H.X. Ca o , F. Bu l l , J. Fu c h s , T. Sc h m u t z e r , 3,000 EST-SNPs loci. – Plant and Animal Genome XIX. G. Jo v t c h e v , A. Pe c i n k a , P. De a r , U. Sc h o l z & I. Sc h u b e r t : Ge- Conference, San Diego/USA, 15.-19.01.2011. nome size evolution in the genus Genlisea from half of the A. thaliana genome to the twenty-fold. – “Plant Ge- nome Evolution” (A Current Opinion Conference), Ams- terdam/The Netherlands, 04.-06.09.2011. P219. We i g e l t , K., R.C. Me y e r , D. Ri e w e , B. Eb e r t , J. Li s e c , R. Sc h m i d t , L. Wi l l m i t z e r & T. Al t m a n n : GABI-GNADE: Genome-wide

207 Vom Institut organisierte Tagungen und Veranstaltungen/Conferences and Meetings Organised by IPK

Vom Institut organisierte Tagungen und Veranstaltungen/ Conferences and Meetings Organised by IPK

2010 Tag der offenen Tür in der Genbank Teilsammlungen Nord zum Motto „2010 – Jahr der Biologischen Vielfalt“ Veranstaltung zur Verabschiedung des Administrativen 5. Juni 2010, Malchow/Poel Leiters, Herrn Bernd Eise und zur Einführung seiner Nach- ca. 200 Teilnehmer folgerin, Frau Sybille-Andrea Lorenz 21. Januar 2010, Gatersleben GFP-Sommertagung 188 Teilnehmer Öffentliche Sitzung der Abteilung Getreide 9. - 10. Juni 2010, Gatersleben Arbeitstagung der Arbeitsgemeinschaft Saatgut- und Sor- 70 Teilnehmer tenwesen der Gesellschaft für Pflanzenbauwissenschaften (GPW) und der Gesellschaft für Pflanzenzüchtung (GPZ) Tag der offenen Tür „Biodiversität als Herausforderung und „Saatgut als Kulturerbe – Produktion, Nutzung und Erhal- Chance“ und 5. Fest der Begegnung tung“ 12. Juni 2010, Gatersleben 24. - 25. Februar 2010, Gatersleben ca. 500 Teilnehmer ca. 100 Teilnehmer 6th Plant Science Student Conference (PSSC) 10. Sitzung der ECPGR-Arbeitsgruppe Forages 15. - 18. Juni 2010, Gatersleben 28. - 29. April 2010, Malchow/Poel 70 Teilnehmer 33 Teilnehmer Workshop „Bestimmung von Schlafmohn und anderen InWent – Weiterbildung und Entwicklung gGmbH Mohnarten“ für Kriminalämter der BRD „The Role of Plant Genetic Resources in 1. - 2. Juli 2010, Gatersleben Climate Change Adaptation“ 10 Teilnehmer 17. - 22. Mai 2010, Gatersleben und Berlin 16 Teilnehmer 3. Gaterslebener Gespräch „Globale Aspekte der Grünen Gentechnik“ Mini-Symposium Association Studies Task Force (ASTF) 16. - 17. September 2010, Gatersleben 27. Mai 2010, Gatersleben 80 Teilnehmer

Abb. 53 Wie hier am Stand der chine- sischen Mitarbeiter/-innen konnten sich die Besucher über das Leben und die Bräuche in den Heimatlän- dern unserer ausländi- schen Wissenschaftler/-innen informieren bzw. auf kuli- narische Entdeckungsreise gehen. (Foto: B. Schäfer/IPK Gatersleben) Fig. 53 Visitors to the Muliti-cultural Event showed great interest in the life, habits and customs in the home countries of our foreign scientists - exemplary the Chinese community. Es- pecially tempting seemed the specialties from the different countries. (Photo: B. Schäfer/ IPK Gatersleben)

208 European Networking Summer School (ENSS) Plant Epige- 2011 netics 2010 „Epigenetische Genregulation in Pflanzen“ 20. - 24. September 2010, Gatersleben Gaterslebener Begegnung XII „Wachstum und Reifung in 43 Teilnehmer Natur und Gesellschaft” 12. - 14. Mai 2011, Gatersleben Institutstag 2010 110 Teilnehmer Vortragsveranstaltung, Posterpräsentation aller wissenschaftlichen Arbeitsgruppen Tag der offenen Tür „Der Wissenschaftsstandort Malchow 4. - 5. Oktober 2010, Gatersleben stellt sich vor“ ca. 200 Teilnehmer 21. Mai 2011, Arbeitsgruppe Teilsammlungen Nord der IPK- Genbank, Malchow/Peol Gaterslebener Forum zum Wissenschaftsmanagement und ca. 300 Teilnehmer Technologietransfer 15. Oktober 2010, Gatersleben Tag der offenen Tür am Biotechnologie-Campus 22 Teilnehmer Gatersleben 28. Mai 2011, Gatersleben Mini-Symposium „Stem Cell Research and Applications“ ca. 300 Teilnehmer anlässlich der Verabschiedung von Frau Prof. Dr. Anna M. Wobus Institutstag 2011 17. November 2010, Gatersleben Vortragsveranstaltung, Posterpräsentation aller wissenschaftli- 60 Teilnehmer chen Arbeitsgruppen 4. - 5. Oktober 2011, Gatersleben 10th Gatersleben Research Conference (GRC X) ca. 200 Teilnehmer “Sequence-informed Crop Research” 22. - 24. November 2010, Gatersleben und Quedlinburg JSPS-DFG Workshop „Frontiers of Plant Chromosome 160 Teilnehmer Research: Centromeres and Artificial Chromosomes“ 31. Oktober - 3. November 2011, Gatersleben Jahresversammlung BarleyGenomeNet 30 Teilnehmer 26. November 2010, Gatersleben 15 Teilnehmer Festveranstaltung anlässlich der Verleihung der For- schungspreise für Grundlagenforschung und angewandte Forschung des Landes Sachsen-Anhalt 5. Dezember 2011, Gatersleben ca. 100 Teilnehmer

Abb. 54 Die Teilnehmer genießen die herbstliche Atmosphäre am IPK während des gemeinsamen JSPS-DFG-finanzierten Workshops "Frontiers of Plant Chromosome Research: Centromeres and Artificial Chromosomes" vor dem Hörsaal. (Foto: H. Ernst/IPK Gatersleben) Fig. 54 Indian Summer in Gatersle- ben: Participants of the joint JSPS-DFG-financed Workshop "Frontiers of Plant Chromo- some Research: Centromeres and Artificial Chromosomes" in front of IPK's lecture hall. (Photo: H. Ernst/IPK Gatersle- ben)

209 Beteiligung an der Organisation externer Veranstaltungen/Participation in Organising External Meetings

Beteiligung an der Organisation externer Veranstaltungen/ Participation in Organising External Meetings

Thema Zeitpunkt der Veranstalter/Mitorganisatoren Art der Veran- Anzahl Veranstaltung (beteiligte Einrichtungen) staltung (national/ Teilnehmer Ort/Land internat.)

2010

6th International Symposium on 20.-22.03.2010 University of Cambridge and international 120 Integrative Bioinformatics Cambridge Rothamsted Research UK Prof. F. Schreiber Dr. U. Scholz

Systems Biology Graphical Notation 21.-23.04.2010 IPK und Martin-Luther-Universität international 40 Workshop (SBGN 5.5) Wittenberg Halle-Wittenberg Prof. F. Schreiber T. Czauderna

2nd International Symposium 24.-27.04.2010 Alma Mater Studiorum international 400 on Genomics of Plant Genetics Bologna Università di Bologna Resources Italy Prof. A. Graner PD Dr. A. Börner

8th International Wheat Conference 01.-04.06.2010 N.I. Vavilov Research Institute of international 650 St. Petersburg Plant Industry (VIR) Russia International Maize and Wheat Improvement Center (CIMMYT) PD Dr. A. Börner

16th International Conference for 07.-08.06.2010 PPM Pilot Pflanzenöltechnologie international ca. 100 Renewable Resources and Plant Magdeburg Magdeburg e.V. Biotechnology Prof. G. Kunze NAROSSA 2010

European Conference on Machine 20.-24.09.2010 Universitat Politècnica de Catalunga international 460 Learning and Principles and Barcelona BarcelonaTech (UPC) Practice of Knowledge Discovery in Spain Centre National de la Recherche Databases (ECML PKDD) Scientifique C( nrS) Dr. J. Keilwagen

Frontier in Agriculture Proteome 18.11.2010 National Institute of Crop Science international ca. 220 Research Tsukuba PD Dr. H.-P. Mock Japan

210 Thema Zeitpunkt der Veranstalter/Mitorganisatoren Art der Veran- Anzahl Veranstaltung (beteiligte Einrichtungen) staltung (national/ Teilnehmer Ort/Land internat.)

2011

COST Action 871, 08.-11.02.2011 Agrocampus Quest of INHP international 68 CryoPlaNet Final meeting Angers Dr. J. Keller France

7th International Symposium on 21.-23.03.2011 University of Wageningen international ca. 120 Integrative Bioinformatics Wageningen Dr. M. Lange, The Netherlands Dr. U. Scholz, Prof. F. Schreiber

Joint AGRISAFE-EUCARPIA Cereals 21.-23.03.2011 Agricultural Research international 160 Section Workshop for Young Cereal Budapest Institute of the Hungarian Academy Scientists “Climate Change and Hungary of Sciences, Martonvásár Plant Breeding Answers“ PD Dr. A. Börner

Seventh meeting of the ECPGR 10.-12.05.2011 ECPGR Secretariat international 28 Barley Working Group Nicosia Dr. H. Knüpffer Cyprus

Workshop Java framework for 19.05.2011 Martin-Luther-Universität Halle- national 20 statistical analysis and classification Halle/S. Wittenberg of biological sequences (Jstacs) Dr. S. Friedel

First International Symposium on 28.-30.06.2011 Northwest A & F University, international 70 Cryopreservation of Horticultural Shaanxi Yangling Crops in China China Dr. J. Keller

21st International Triticeae Mapping 05.-09.09.2011 Internationale Triticeae Mapping international ca. 300 Initiative Workshop Mexico City Initiative Mexico Dr. N. Stein

Seventh meeting of the ECPGR 06.-08.09.2011 European Cooperative Programme international 22 Allium Working Group Perea, Thessaloniki for Plant Genetic Resources (ECPGR) Greece Dr. J. Keller

Non-conventional Yeast in the 11.-14.09.2011 Institute of Cell Biology, international ca. 200 Postgenomic Era (NCY) Lviv NAS of Ukraine, Ukrainian Society of Ukraine Cell Biology Prof. G. Kunze

7th International Symposium on 18.-22.09.2011 International Society for international 260 In vitro Culture and Horticultural Ghent Horticultural Science, Breeding „Biotechnological Belgium University Ghent, Belgium Advances in In vitro Horticultural Dr. J. Keller Breeding“

6. Fachtagung Arznei- und 19.-22.09.2011 Deutscher Fachausschuss international ca. 150 Gewürzpflanzen Berlin für Arznei-, Gewürz- und Aromapflanzen (DFA) und Landwirtschaftlich-Gärtnerische Fakultät der Humboldt-Universität zu Berlin Dr. U. Lohwasser

Joint EWAC-EUCARPIA Cereals 07.-11.11.2011 Institute of Field and Vegetable international 60 Section Conference „Development Novi Sad Crops, Novi Sad and Utilisation of Cereal Stocks for Serbia PD Dr. A. Börner Gene Identification and Molecular Mapping“

Parlamentarischer Abend 12.12.2011 Ministerium für Wissenschaft und national 70 „Pflanzen-basierte Bioökonomie: Berlin Wirtschaft Sachsen-Anhalt, Wertstoffproduktion und IPB Halle/S., IPK Gatersleben Ernährungssicherung“

211 Ehrungen, Preise/Honours, Awards

Ehrungen, Preise/Honours, Awards

2010 Audience Award – Best Poster

Der Biologe Dr. Nils Stein hat am 15. März 2010 den erstmals Young-Min Kim, IPK, Abt. Physiologie und Zellbiologie, Ar- verliehenen Günter und Anna Wricke-Forschungspreis erhal- beitsgruppe Molekulare Pflanzenernährung. ten. Damit wurden seine Arbeiten zur Genforschung an Getrei- de durch die gleichnamige Stiftung gewürdigt (http://www. M.Sc. Manuela Nagel erhielt auf dem 29th International Seed wricke-stiftung.de). Testing Association (ISTA) Congress, der vom 15. bis 18. Juni 2010 in Köln stattfand, den „Seed Symposium Award 2010“ für Auf der 45. Vortragstagung der Deutschen Gesellschaft für Qua- hervorragende Leistungen ihrer Darstellung. litätsforschung präsentierte sich Manuela Nagel mit einem Poster, das am 23. März 2010 als „Hervorragender wissenschaft- Honorable mention of BEST Performer (2nd place) in the licher Posterbeitrag“ ausgezeichnet wurde. Die Veranstaltung DREAM5 international initiative of Columbia University and fand in Berlin-Dahlem statt. IBM, challenge: „Transcription-Factor/DNA-Motif Recognition“ for Jens Keilwagen, Jan Grau, Ivo Grosse, Stefan Posch, No- Am 25. März 2010 erhielt Jemanesh Kifatew Haile den „Jeanie vember 2010. Borlaug Laube Women in Triticum Award 2010“. Die äthiopi- sche Wissenschaftlerin arbeitet in der Arbeitsgruppe Gen- und Genomkartierung bei Dr. M. Röder. 2011

Bei der 6th Plant Science Student Conference (PSSC), die vom Die Nationale Akademie der Wissenschaften Leopoldina 15. bis 18. Juni 2010 im IPK stattfand, wurden folgende Preise ehrte ihre Mitglieder Frau Prof. Dr. Anna M. Wobus und Herrn vergeben: Prof. Dr. Ulrich Wobus mit der Cothenius-Medaille in Gold. Sie erhielten diese Ehrung für ihr wissenschaftliches Lebenswerk. Jury Award ‑ Best Talks Die Medaillen wurden im Rahmen der feierlichen Eröffnung der Leopoldina-Jahresversammlung am 23. September 2011 in 1. Platz: Rico Lippmann, IPK, Abt. Physiologie und Zellbiolo- Halle/S. überreicht. gie, Arbeitsgruppe Angewandte Biochemie; Dr. Manuela Nagel, Dr. Astrid Junker und Nicolai Nürk wur- 2. Platz: Naser Poursarebani, IPK, Abt. Genbank, Arbeits- den am 5. Oktober 2011 mit dem „Beagle Award“ ausgezeich- gruppe Genomdiversität; net. Sie erhielten diesen Preis, der an Nachwuchswissenschaft- ler des IPK vergeben wird, für ihre wissenschaftliche Arbeit 3. Platz: Katarzyna Plasun, IPK, Abt. Physiologie und Zellbio- und ihr soziales Engagement. Die Preisverleihung erfolgte im logie, Arbeitsgruppe Pflanzliche Reproduktionsbiologie. Anschluss an den Institutstag durch den Geschäftsführenden Direktor und das Student Board. Jury Award ‑ Best Poster Am 5. Dezember 2011 wurde der Forschungspreis für ange- 2. Platz: Tina Seidensticker, IPK, Abt. Genbank, Arbeitsgrup- wandte Forschung des Landes Sachsen-Anhalt an den langjäh- pe Pflanzliche Baupläne; rigen Mitarbeiter des IPK und Leiter der Arbeitsgruppe Hefege- netik, Herrn Prof. Dr. Gotthard Kunze, verliehen und im Rah- 3. Platz: Anne Bohner, IPK, Abt. Physiologie und Zellbiologie, men einer Festveranstaltung durch Herrn Staatssekretär Marco Arbeitsgruppe Molekulare Pflanzenernährung. Tullner, Ministerium für Wissenschaft und Wirtschaft des Landes Sachsen-Anhalt, überreicht. Audience Award – Best Talk Von der Ag Dateninspektion nahmen Dr. Jens Keilwagen und Katarzyna Plasun, IPK, Abt. Physiologie und Zellbiologie, Dr. Michael Seifert erfolgreich am 6. „Bioinformatics DREAM Arbeitsgruppe Pflanzliche Reproduktionsbiologie. 2011“ Wettbewerb teil. Gemeinsam mit sechs anderen Teams errangen beide unabhängig voneinander den 1. Platz.

212 Arbeitsaufenthalte von Gästen im IPK/ Guest Researchers at the IPK (ab einer Woche, ohne Schüler, Praktikanten, Studenten)

Abteilung Genbank Dr. Cedric Moisy, MTT/BI Plant Genomics Laboratory, Hel- sinki, Finnland, 22.03.2010 bis 26.03.2010, Eigenfinanzierung Dr. Sven Gottwald, Justus-Liebig-Universität, Gießen, (Dr. N. Stein/Arbeitsgruppe Genomdiversität). 01.01.2010 bis 28.02.2011, Eigenfinanzierung (Prof. Dr. A. Gra- ner, Dr. N. Stein/Arbeitsgruppe Genomdiversität). Dr. Outi Manninen, MTT/Agrifood Research, Helsinki, Finnland, 22.03.2010 bis 26.03.2010, Eigenfinanzeirung (Dr. N. Stein/Ar- Dr. Dragan Perovic, Julius Kühn-Institut Quedlinburg, Qued- beitsgruppe Genomdiversität). linburg, 01.01.2010 bis 30.04.2010, Finanzierung durch JKI Quedlinburg; 01.06.2010 bis 31.12.2010, Eigenfinanzierung Dr. Marco Maccaferri, Department of Agro-Environmental (Dr. N. Stein/Arbeitsgruppe Genomdiversität). Science and Technology, University of Bologna, Bologna, Itali- en, 22.03.2010 bis 26.03.2010, Finanzierung durch University of Salar Shaaf, Department of Agronomy and Plant, University of Te- Bologna (Dr. N. Stein/Arbeitsgruppe Genomdiversität). heran, Teheran, Iran, 11.02.2010 bis 25.09.2010, Finanzierung durch iranische Regierung (Dr. B. Kilian/Arbeitsgruppe Genomdiversität). Dr. Bu-Jun Shi, Australian Center for Plant Functional Geno- mics (ACPFG), Adelaide, Australien, 27.02.2011 bis 02.03.2011, Agostino Fricano, Università degli Studi di Milano, Lodi, Finanzierung durch University of Adelaide (Dr. N. Stein/Arbeits- Italien, 22.03.2010 bis 26.03.2010, Finanzierung durch BMBF gruppe Genomdiversität). (Dr. N. Stein/Arbeitsgruppe Genomdiversität). Oskar Obidiegwu, University Hohenheim, Institute of Plant Benedikt Athmer, 01.04.2010 bis 31.12.2011, Eigenfinanzie- Breeding, Seed Science and Population Genetics, Stuttgart- rung (Dr. N. Stein/Arbeitsgruppe Genomdiversität). Hohenheim, 28.08.2011 bis 02.09.2011, Finanzierung durch IPK und University of Hohenheim (Dr. B. Kilian/Arbeitsgruppe Dr. Jan Bartos, Laboratory of Molecular Cytogenetics and Genomdiversität). Cytometry, Institute of Experimental Botany, Olomouc, Tsche- chische Republik, 22.03.2010 bis 26.03.2010, Eigenfinanzierung Ben-Israel Imri, Hebrew University, Faculty of Agricultural (Dr. N. Stein/Arbeitsgruppe Genomdiversität). Science, Jerusalem, Israel, 19.06.2011 bis 26.06.2011, Finanzie- rung durch IPK und Eigenfinanzierung (Dr. B. Kilian/Arbeits- Ping Yang, 24.08.2010 bis 31.08.2010, Eigenfinanzierung gruppe Genomdiversität). (Dr. N. Stein/Arbeitsgruppe Genomdiversität). Dr. Anna Filatenko, Vavilov Institute (VIR), St. Petersburg, Dr. Daniela Schulte, 01.02.2010 bis 31.12.2010, Eigenfinanzie- Russland, 22.05.2011 bis 31.10.2011, Finanzierung durch DFG rung (Dr. N. Stein/Arbeitsgruppe Genomdiversität). (Dr. B. Kilian/Arbeitsgruppe Genomdiversität).

Therese Gradin, School of Life Sciences, Södertörn Universi- Dr. Maria Munoz Amatriain, University of Minnesota, De- ty, Södertörn, Schweden, 01.02.2010 bis 28.02.2010, Finanzie- partment of Agronomy and Plant Genetics, Minnesota, USA, rung durch Södertörn University (Dr. N. Stein/Arbeitsgruppe 06.06.2011 bis 17.07.2011, Finanzierung durch University of Genomdiversität). Minnesota (Dr. N. Stein/Arbeitsgruppe Genomdiversität und Dr. U. Scholz/Arbeitsgruppe Bioinformatik und Informations- Dr. Fedor A. Konovalov, N. I. Vavilov Institute of General Ge- technologie). netics, Moskau, Russland, 01.03.2010 bis 31.05.2010, Finanzie- rung durch DFG; 18.11.2010 bis 18.12.2010, Eigenfinanzierung; Dr. Marzena Kurowska, University of Silesia, Faculty of Biology 04.06.2011 bis 19.06.2011, IPK (Dr. B. Kilian/Arbeitsgruppe Ge- and Environmental Protection, Department of Genetics, Ka- nomdiversität). towice, Polen, 29.10.2011 bis 31.01.2012, Finanzierung durch IAEA-Stipendium (Dr. N. Stein/Arbeitsgruppe Genomdiversi- Dr. Marianna Paquariello, University of Modena, Modena, tät). Italien, 15.03.2010 bis 30.05.2010; 28.08.2011 bis 27.09.2011, Eigenfinanzierung (Dr. N. Stein, Dr. A. Himmelbach/Arbeits- Dr. Helmy Mohamed Youssef, Plant Physiology & Biotech De- gruppe Genomdiversität). partment, Faculty of Agriculture, Kairo, Ägypten, 09.05.2010 bis 09.07.2010, Finanzierung durch DAAD-Stipendium Dr. Minne Turktas, Szbzrci University of Istanbul, Istanbul, Tür- (Dr. T. Schnurbusch/Arbeitsgruppe Pflanzliche Baupläne). kei, 22.03.2010 bis 26.03.2010, Eigenfinanzierung (Dr. N. Stein/ Arbeitsgruppe Genomdiversität).

213 Arbeitsaufenthalte von Gästen im IPK/Guest Researchers at the IPK

Arash Fazeli, University of Sari, Sari, Iran, 13.04.2011 bis Mai Allam, National Research Center (NRC), Plant Biotechno- 12.10.2011, Finanzierung durch Ministry of Science, Research logy Department, Kairo, Ägypten, 04.04.2011 bis 03.04.2014, & Technology, Iran; 13.10.2011 bis 31.12.2011, Eigenfinan- Finanzierung durch DAAD (Dr. A. Börner/Arbeitsgruppe Res- zierung; 01.01.2012 bis 29.02.2012, Finanzierung durch IPK sourcengenetik und Reproduktion). (Dr. T. Schnurbusch/Arbeitsgruppe Pflanzliche Baupläne). Dr. Isaac Daniel, University of Agriculture, Department of Plant Dr. Yuri Shavrukov, Australian Centre for Plant Functional Ge- Breeding & Seed Technology, Abeokuta, Nigeria, 01.07.2011 bis nomics, Glen Osmond, Australien, 19.05.2010 bis 21.05.2010, 26.09.2011, Finanzierung durch ein Stipendium der Humboldt- Eigenfinanzierung (Dr. T. Schnurbusch/Arbeitsgruppe Pflanz- Stiftung (Dr. A. Börner/Arbeitsgruppe Ressourcengenetik und liche Baupläne). Reproduktion).

Lucia Stimolo, Institute of Plant Genetics, Genebank Bari, Bari, Serik Jantassov, Kasakh Research Institute of Potato and Vege- Italien, 23.08.2010 bis 27.08.2010, Eigenfinanzierung (Dr. U. tables Production, Almaty Oblast, Karasay Rayon, Kasachstan, Lohwasser/Arbeitsgruppe Ressourcengenetik und Reproduk- 11.09.2011 bis 17.09.2011, Finanzierung durch GIZ Bonn/Esch- tion). born (Dr. U. Lohwasser/Arbeitsgruppe Ressourcengenetik und Reproduktion). Mian Abdur Rehman Arif, University of Arid Agriculture, Ra- walpindi, Pakistan, 01.01.2010 bis 31.03.2012, Finanzierung Dr. Malgorzata Pelc, Warsaw University of Life Science, De- durch DAAD (Dr. A. Börner/Arbeitsgruppe Ressourcengenetik partment of Vegetable and Medicinal Plants, Warschau, Polen, und Reproduktion). 07.04.2010 bis 28.05.2010, Finanzierung durch COST Action 871 (Dr. J. Keller/Arbeitsgruppe In vitro-Erhaltung und Cryo- Khalil Zaynali Nezhad, Department of Agronomy and Plant Lagerung). Breeding, College of Agriculture, Isfahan Institute of Technolo- gy, Isfahan, Iran, 01.01.2010 bis 30.09.2010, Finanzierung durch Marta Olas-Sochacka, Research Institute of Vegetable Crops iranische Regierung (Dr. A. Börner/Arbeitsgruppe Ressourcen- (RIVC), Warschau, Polen, 19.10.2010 bis 18.11.2010, Finanzie- genetik und Reproduktion). rung durch COST Action 871 (Dr. J. Keller/Arbeitsgruppe In vitro-Erhaltung und Cryo-Lagerung). Kerstin Neumann, 01.01.2010 bis 31.08.2010, Eigenfinanzie- rung (Dr. A. Börner/Arbeitsgruppe Ressourcengenetik und Re- Dr. Magfrat Muminova, Academy of Science, Institute of Ge- produktion). netics and Plant Experimental Biology, Tashkent, Usbekistan, 12.09.2011 bis 11.12.2011, Finanzierung durch DAAD (Dr. J. Kel- Dr. Oxana Dobrovolskaya, Institute of Cytology and Gene- ler/Arbeitsgruppe In vitro-Erhaltung und Cryo-Lagerung). tics, Nowosibirsk, Russland, 02.03.2010 bis 03.04.2010 und 31.10.2010 bis 23.12.2010, Finanzierung durch DFG (Dr. A. Bör- Andreas Stephanik, 01.02.2010 bis 31.12.2010, Eigenfinanzie- ner/Arbeitsgruppe Ressourcengenetik und Reproduktion). rung (Dr. H. Knüpffer/Arbeitsgruppe Genbankdokumentation).

Dr. Annette Weidner, 01.01.2010 bis 30.06.2010, Eigenfinan- Tamar Jinjikhadze, Ilia State University Tbilisi, Botanical Gar- zierung (Dr. A. Börner/Arbeitsgruppe Ressourcengenetik und den and Institute of Botany, Cultural Flora Department, Tbilisi, Reproduktion). Georgien, 19.03.2011 bis 29.03.2011, Finanzierung durch Tbilisi Botanical Garden (Dr. H. Knüpffer/Arbeitsgruppe Genbankdo- Dr. Abdolrazagh Danesh-Shahraki, University of Shahrekord, kumentation). Department of Agronomy and Plant Breeding, Faculty of Agri- culture, Shahrekord, Iran, 13.07.2010 bis 29.07.2010, Finanzie- Solomon Benor, Debub University, Department of Plant Sciences, rung durch Shahrekord University (Dr. A. Börner/Arbeitsgruppe Awassa, Äthiopien, 01.01.2010 bis 30.09.2011, Finanzierung durch Ressourcengenetik und Reproduktion). DAAD (Dr. F. Blattner/Arbeitsgruppe Experimentelle Taxonomie).

Dr. Natalia Tikhenko, St. Petersburg State University, Biologi- Christina Baier, 01.09.2010 bis 31.03.2011 und 01.07.2011 bis cal Research Institute, St. Petersburg, Russland, 04.04.2010 bis 31.12.2011, Eigenfinanzierung (Dr. F. Blattner/Arbeitsgruppe 14.04.2010, Eigenfinanzierung (Dr. A. Börner/Arbeitsgruppe Experimentelle Taxonomie). Ressourcengenetik und Reproduktion). Dr. Anna A. Filatenko, N. I. Vavilov-Institute (VIR), St. Peters- Klaudia Kruppa, Martonvásár, Agricultural Research Institute, burg, Russland, 15.02.2010 bis 10.03.2010, Eigenfinanzierung Martonvásár, Ungarn, 10.01.2011 bis 09.04.2011, Finanzierung (Dr. F. Blattner/Arbeitsgruppe Experimentelle Taxonomie). durch Agricultural Research Institute Martonvásár (Dr. A. Bör- ner/Arbeitsgruppe Ressourcengenetik und Reproduktion). Prof. Dr. Konrad Bachmann, 01.01.2010 bis 31.05.2011, Eigenfinanzierung (Dr. F.B lattner/Arbeitsgruppe Experimentel- le Taxonomie).

214 Dr. Reinhard Fritsch, 01.01.2010 bis 30.04.2012, Finanzierung Gurmeet Kaur, Jammu Universität, Indien, 17.10.2010 bis durch DFG (Dr. F. Blattner/Arbeitsgruppe Taxonomie pflanzen- 01.12.2010, Finanzierung durch DAAD (Dr. A. Houben/Arbeits- genetischer Ressourcen). gruppe Chromosomenstruktur und -funktion).

Maria Cuacos, University Madrid, Madrid, Spanien, 01.06.2011 bis 31.08.2011, Finanzierung durch University Madrid, Minis- Abteilung Cytogenetik und Genomanalyse terio de Educacion (Dr. A. Houben/Arbeitsgruppe Chromo- somenstruktur und -funktion). Emilia Cincu, Humboldt-Universität zu Berlin, Berlin, 31.05.2010 bis 04.06.2010, Eigenfinanzierung (Dr. J. Fuchs/Arbeitsgruppe Aretuza Sousa, Ludwig-Maximilians-Universität, München, Karyotypevolution). 28.02.2011 bis 11.03.2011, Finanzierung durch Ludwig-Maxi- milians-Universität München (Dr. A. Houben/Arbeitsgruppe Aslihan Temel, Universität Istanbul, Türkei, 04.01.2010 bis Chromosomenstruktur und -funktion). 30.09.2010, Finanzierung durch Stipendium der Universität Istanbul (Prof. Dr. I. Schubert/Arbeitsgruppe Karyotypevolution). Maryam Sanei, 01.07.2011 bis 01.10.2011, Eigenfinanzierung (Dr. A. Houben/Arbeitsgruppe Chromosomenstruktur und Katharina Seeliger, KIT Campus Süd, Institut für Botanik II, -funktion). Karlsruhe, 11.04.2010 bis 17.04.2010, Eigenfinanzierung (Prof. Dr. I. Schubert/Arbeitsgruppe Karyotypevolution). Erwann Caillieux, Institut de Biologie de l’ école normale su- périeure (IBENS), Paris, Frankreich, 10.10.2011 bis 28.10.2011, Prof. Takashi R. Endo, Universität Kyoto, Japan, 20.11.2010 Finanzierung durch DAAD (Dr. A. Houben/Arbeitsgruppe bis 25.11.2010, Finanzierung durch IPK (Prof. Dr. I. Schubert/ Chromosomenstruktur und -funktion). Arbeitsgruppe Karyotypevolution). Takayoshi Ishii, Tottori University, Laboraty of Molecular Breed- Dr. Insa Schröder, Martin-Luther-Universität Halle-Wittenberg, ing Arid Lund Research Center, Tottori, Japan, 17.10.2011 bis Halle/S., 01.01.2011 bis 30.06.2011, Finanzierung durch Koope- 30.10.2011, Eigenfinanzierung (Dr. A. Houben/Arbeitsgruppe rationsvertrag mit der MLU Halle/S. (Prof. Dr. I. Schubert/Ar- Chromosomenstruktur und -funktion). beitsgruppe Karyotypevolution). Johanna Acevedo-Garcia, Max-Planck-Institut für Pflanzen- Prof. Dr. Anna Magdalene Wobus, 01.01.2011 bis 31.03.2012, züchtungsforschung Köln, Department of Plant Microbe Interac- Eigenfinanzierung (Prof. Dr. I. Schubert/Arbeitsgruppe Karyo- tions, Köln, 31.07.2011 bis 13.08.2011, Eigenfinanzierung (Dr. A. typevolution). Houben/Arbeitsgruppe Chromosomenstruktur und -funktion).

Tran Duc Trung, Plant Resource Center (PRC), Division of Agri- Samuel Amiteye, International Max Planck Research School, cultural Biodiversity, Vietnam Academy of Agricultural Science, Asutsuare, Ghana, 01.01.2010 bis 22.02.2012, Finanzierung Hanoi, Vietnam, 17.09.2011 bis 30.09.2014, Finanzierung durch durch International Max Planck Research School (Dr. T. Sharbel/ MOET-Stipendium Vietnam (Prof. Dr. I. Schubert/Arbeitsgruppe Arbeitsgruppe Apomixis). Karyotypevolution). Thomas Thiel, International Max Planck Research School, Jena, Dr. Thi Ha Giang Vu, Max-Planck-Institut für Pflanzenzüch- 01.01.2010 bis 31.12.2011, Finanzierung durch International tungsforschung, Köln, 25.05.2011 bis 03.06.2011 und 30.09.2011 Max Planck Research School/Max-Planck-Institut für Chemi- bis 10.10.2012, Eigenfinanzierung (Prof. Dr. I. Schubert/Arbeits- sche Ökologie, Jena (Dr. T. Sharbel/Arbeitsgruppe Apomixis). gruppe Karyotypevolution). Susanne Scheffknecht, Universität Wien, Abteilung Pharma- Miriam Gonzalez Garcia, Universität Madrid, Spanien, kognosie, Wien, Österreich, 01.08.2010 bis 14.08.2010, Finan- 02.05.2010 bis 02.08.2010, Finanzierung durch Universität Ma- zierung durch FWF (Dr. T. Sharbel/Arbeitsgruppe Apomixis). drid, Ministerio de Educacion, Spanien (Dr. A. Houben/Arbeits- gruppe Chromosomenstruktur und -funktion). Charlotte Scheriau, Heidelberg Institute for Plant Science, Bio- diversity and Plant Systematics, Universität Heidelberg, Heidel- Francesco Agueci, 01.01.2010 bis 30.06.2010, Eigenfinanzie- berg, 28.02.2010 bis 06.03.2010, Finanzierung durch DFG (Dr. T. rung (Dr. A. Houben/Arbeitsgruppe Chromosomenstruktur Sharbel/Arbeitsgruppe Apomixis). und -funktion). Marcin Piwczynski, Max-Planck-Institut für Chemische Öko- Dr. Elena Evtushenko, Institute of Chemical Biology and Funda- logie Jena, Jena, 30.8.2010 bis 27.09.2010, Finanzierung durch mental Medicine, Laboratory of Molecular Genetics, Nowosibirsk, Nicolaus Copernicus University, Poland (Dr. T. Sharbel/Arbeits- Russland, 01.09.2010 bis 29.11.2010, Finanzierung durch DFG (Dr. gruppe Apomixis). A. Houben/Arbeitsgruppe Chromosomenstruktur und -funktion).

215 Arbeitsaufenthalte von Gästen im IPK/Guest Researchers at the IPK

Dr. Diego Hojsgaard, Universität Wien, Department of Syste- K. A. Geetha, Directorate of Medicinal and Aromatic Plants Re- matic and Evolutionary Botany, Wien, Österreich, 17.10.2010 search, Boriavi, Indien, 02.05.2011 bis 31.07.2011, Finanzierung bis 23.10.2010 und 23.03.2011 bis 31.05.2011, Finanzierung durch Stipendium der World Bank (Dr. T. Sharbel/Arbeitsgruppe durch FWF (Dr. T. Sharbel/Arbeitsgruppe Apomixis). Apomixis).

Dr. Christoph Dobes, Universität Wien, Abteilung Pharmakog- Dr. Marta Puente Molins, 15.06.2011 bis 15.03.2012, Eigenfi- nosie, Wien, Österreich, 01.08.2010 bis 08.08.2010, Finanzie- nanzierung (Dr. T. Sharbel/Arbeitsgruppe Apomixis). rung durch FWF (Dr. T. Sharbel/Arbeitsgruppe Apomixis). Dr. Insa Schröder, Martin-Luther-Universität Halle-Wittenberg, Ingrid Jordon-Thadeus, Heidelberg Institute for Plant Science, Halle/S., 01.01.2010 bis 31.12.2010, Finanzierung durch Koope- Biodiversity and Plant Systematics, Universität Heidelberg, Hei- rationsvertrag mit der MLU Halle (Prof. Dr. A.M. Wobus/Arbeits- delberg, 28.02.2010 bis 06.03.2010, Finanzierung durch DFG gruppe In vitro-Differenzierung). (Dr. T. Sharbel/Arbeitsgruppe Apomixis). Anna Daniel-Wojcik, 01.03.2010 bis 31.08.2010, Eigenfinanzie- Dr. Lars-Gernot Otto, 01.01.2010 bis 31.07.2010, 11.08.2010 rung (Prof. Dr. A.M. Wobus/Arbeitsgruppe In vitro-Differenzie- bis 14.02.2011 und 15.08.2011 bis 31.12.2011, Eigenfinanzie- rung). rung (Dr. T. Sharbel/Arbeitsgruppe Apomixis). Dung Phuong Le, Martin-Luther-Universität Halle-Wittenberg, Giulio Galla, University of Padova, Department of Enviromen- Halle/S., 24.09.2011 bis 30.09.2015, Finanzierung durch MOET/ tal Agronomy & Crop Science, Padua, Italien, 08.03.2010 bis DAAD-Stipendium (Dr. R. Schmidt/Arbeitsgruppe Genom- 12.04.2010 und 05.08.2011 bis 26.08.2011, Eigenfinanzierung plastizität). (Dr. T. Sharbel/Arbeitsgruppe Apomixis). Astrid Bruchmüller, 01.04.2010 bis 30.09.2010, Eigenfinanzie- Francisco Valverde, University of Granada, Granada, Spanien, rung (Dr. M. Mette/Arbeitsgruppe Epigenetik). 21.02.2011 bis 18.04.2011, Finanzierung durch University of Granada (Dr. T. Sharbel/Arbeitsgruppe Apomixis). Alexandra Plochotnikova, University of Vilnius, Institute of Biotechnology, Vilnius, Litauen, 06.06.2011 bis 18.06.2011, Ei- Javier Morillas, University of Granada, Granada, Spanien, genfinanzierung und Finanzierung durch IPK (Dr. M. Mette/ 21.02.2011 bis 18.04.2011, Finanzierung durch University of Arbeitsgruppe Epigenetik). Granada (Dr. T. Sharbel/Arbeitsgruppe Apomixis). Simona Jachimoviciute, University of Vilnius, Institute of Bio- Ana Sofia Rois, Instituto Superior de Agronomia (ISA), Lissa- technology, Vilnius, Litauen, 06.06.2011 bis 18.06.2011, Eigenfi- bon, Portugal, 24.01.2011 bis 04.02.2011, Finanzierung durch nanzierung und Finanzierung durch IPK (Dr. M. Mette/Arbeits- Instituto Superior de Agronomia (Dr. T. Sharbel/Arbeitsgruppe gruppe Epigenetik). Apomixis). Dr.-Ing. Alexander Ihlow, Technische Hochschule Ilmenau, Anna Weber, Poznań University of Life Sciences, Poznań, Polen, Ilmenau, 01.01.2010 bis 30.11.2011, Finanzierung durch TH 14.08.2011 bis 10.09.2011, Finanzierung durch EU COST Action Ilmenau (Dr. P. Schweizer/Arbeitsgruppe Transkriptomanalyse). (Dr. T. Sharbel/Arbeitsgruppe Apomixis). Stephan Marzin, 01.01.2010 bis 31.01.2010, Eigenfinanzierung Lúbos Majeský, University of Olomouc, Olomouc, Tschechische (Dr. P. Schweizer/Arbeitsgruppe Transkriptomanalyse). Republik, 12.09.2011 bis 12.12.2011, Finanzierung durch Univer- sity of Olomouc (Dr. T. Sharbel/Arbeitsgruppe Apomixis). Luo Liu, 01.01.2010 bis 30.06.2010, Eigenfinanzierung (Dr. P. Schweizer/Arbeitsgruppe Transkriptomanalyse). John Thomsen Lovell, Colorado State University, Fort Collins, USA, 25.09.2011 bis 05.12.2011, Finanzierung durch DFG-Sti- Ernst Metzner, Martin-Luther-Universität Halle-Wittenberg, pendium (Dr. T. Sharbel/Arbeitsgruppe Apomixis). Halle/S., 01.01.2010 bis 31.07.2010, Eigenfinanzierung (Dr. P. Schweizer/Arbeitsgruppe Transkriptomanalyse). Jana Koperdakova, Pavol Jozef Šafárik University, Institute of Biology and Ecology, Košice, Slovakische Republik, 01.09.2011 Dr. Clara Pliego Prieto, Imperial College London, Großbri- bis 19.09.2011, Finanzierung durch EU COST Action (Dr. T. Shar- tannien, 20.09.2010 bis 20.10.2010, Eigenfinanzierung (Dr. P. bel/Arbeitsgruppe Apomixis). Schweizer/Arbeitsgruppe Transkriptomanalyse).

E. Eradasappa, Central Potato Research Station, Sahaynagar, In- Gerald Siegwart, Universität für Bodenkultur, Wien, Österreich, dien, 14.10.2011 bis 11.01.2012, Finanzierung durch ein Stipen- 20.09.2010 bis 16.10.2010, Eigenfinanzierung (Dr. P. Schweizer/ dium der World Bank (Dr. T. Sharbel/Arbeitsgruppe Apomixis). Arbeitsgruppe Transkriptomanalyse).

216 Tobias Baum, Fraunhofer-Institut für Fabrikbetrieb und -auto- Dr. Urte Schlüter, Friedrich-Alexander-Universität Erlan- matisierung, Magdeburg, 09.03.2011 bis 30.06.2011, Finanzie- gen-Nürnberg, Erlangen, 06.06.2011 bis 10.06.2011, Eigenfi- rung durch BMBF (Dr. P. Schweizer/Arbeitsgruppe Transkrip- nanzierung (Dr. U. Scholz/Arbeitsgruppe Bioinformatik und tomanalyse). Informationstechnologie).

Jemanesh Kifetew Haile, Ethiopian Institute of Agricultural Maria Munoz Amatriain, University of Minnesota, Department Research, Addis Abeba, Äthiopien, 01.01.2010 bis 30.04.2012, of Agronomy and Plant Genetics, Minnesota, USA, 06.06.2011 Finanzierung durch DAAD-Stipendium (Dr. M. Röder/Arbeits- bis 17.07.2011, Finanzierung durch University of Minnesota gruppe Gen- und Genomkartierung). (Dr. U. Scholz/Arbeitsgruppe Bioinformatik und Dr. N. Stein/ Ar- beitsgruppe Genomdiversität). Rajneesh Paliwal, Banaras Hindu University, Varanasi, Indien, 01.01.2010 bis 30.06.2010, Finanzierung durch DAAD-Stipendi- um (Dr. M. Röder/Arbeitsgruppe Gen- und Genomkartierung). Abteilung Molekulare Genetik Dr. Jose Luis Diaz De Léon, Universidad de Baja California, La Paz, Mexiko, 28.07.2010 bis 29.09.2010, Finanzierung durch Mehrana Koohi, Shahrekord University, Department of Plant CONACYT-BMBF; 04.09.2011 bis 04.10.2011, Finanzierung Breeding, College of Agriculture, Shahrekord, Iran, 10.01.2010 durch BMBF; 05.10.2011 bis 13.10.2011, Eigenfinanzierung (Dr. bis 31.08.2010 und 01.12.2010 bis 30.09.2011, Finanzierung M. Röder/Arbeitsgruppe Gen- und Genomkartierung). durch Iranian Ministry of Science, Research and Technology (Prof. Dr. T. Altmann, Dr. R. Meyer/Arbeitsgruppe Heterosis). Aiman Absattarova, Kazakh Scientific Production Center of Farming and Plant Growth, Almalybak, Kasachstan, 11.01.2010 Hea-Jung Jeon, Kyung Hee University, Department of Hor- bis 19.03.2010, Finanzierung durch IAOE-Stipendium (Dr. M. ticultural Biotechnology, College of Life Science, Youngin-si, Röder/Arbeitsgruppe Gen- und Genomkartierung). Republik Korea, 27.09.2010 bis 26.09.2013, Finanzierung durch Stipendium der koreanischen Regierung (Prof. Dr. T. Altmann/ Dr. Elena Salina, Institute of Cytology and Genetics, Nowosibirsk, Arbeitsgruppe Heterosis). Russland, 04.10.2010 bis 23.10.2010, Finanzierung durch BLE-Pro- jekt (Dr. M. Röder/Arbeitsgruppe Gen- und Genomkartierung). Dr. Michaela Ernst, Forschungszentrum Jülich, 01.09.2010 bis 31.03.2011 und 25.11.2011 bis 31.05.2012, Eigenfinanzierung Dr. Thelma Castellanos, Universidad de Baja California, La (Prof. Dr. T. Altmann/Arbeitsgruppe Heterosis). Paz, Mexiko, 28.07.2010 bis 29.09.2010, Finanzierung durch CONACYT-BMBF (Dr. M. Röder/Arbeitsgruppe Gen- und Ge- Dr. Marc Strickert, Universität Siegen, Fakultät Medieninfor- nomkartierung). matik, 01.02.2010 bis 31.10.2010, Eigenfinanzierung (Dr. W. Weschke/Arbeitsgruppe Samenentwicklung). Dr. Irina Leonova, Institute of Cytology and Genetics, Nowosi- birsk, Russland, 10.10.2010 bis 09.12.2010, Finanzierung durch Dr. Christiane Seiler, 01.07.2010 bis 31.10.2010, Eigenfinan- DFG (Dr. M. Röder/Arbeitsgruppe Gen- und Genomkartierung). zierung (Dr. W. Weschke, Dr. N. Sreenivasulu/Arbeitsgruppe Sa- menentwicklung). Merit Mora, Universidad de Baja California, La Paz, Mexiko, 03.08.2010 bis 29.09.2010, Finanzierung durch CONACYT-BMBF Dr. Manoj Prasad, National Institute of Plant Genome Re- (Dr. M. Röder/Arbeitsgruppe Gen- und Genomkartierung). search, Aruna Asaf Ali Marg, New Delhi, Indien, 12.02.2010 bis 28.02.2010 und 18.11.2010 bis 27.11.2010, Finanzierung durch Mehmoona Ilyas, University of Arid Agriculture, Shamsabad, DLR (Dr. N. Sreenivasulu/Arbeitsgruppe Samenentwicklung). Pakistan, 18.08.2011 bis 17.02.2012, Finanzierung durch Re- search Fellowship of Higher Education Commission (Dr. M. Rö- Nicole Staroske, 01.04.2011 bis 30.04.2011 und 01.08.2011 bis der/Arbeitsgruppe Gen- und Genomkartierung). 31.12.2011, Eigenfinanzierung (Dr. W. Weschke/Arbeitsgruppe Samenentwicklung). Dr. Uttam Kumar, Plant Tissue Culture & Molecular Biology (TERI), New Delhi, Indien, 01.06.2011 bis 22.08.2012, Finanzie- Van Tran Thi Thuy, Agricultural Science Institute of Northern rung durch BMBF (Dr. M. Röder/Arbeitsgruppe Gen- und Ge- Central Vietnam, Vinh City, Vietnam, 21.08.2011 bis 31.08.2014, nomkartierung). Finanzierung durch Ministry of Education and Training (Dr. W. Weschke/Arbeitsgruppe Samenentwicklung). Dr. Malgorzata Czernicka, University of Agriculture Krakow, Department of Genetics, Plant Breeding and Seed Science, Maria Isabel Mora Ramirez, Universidad de Costa Rica, San Krakow, Polen, 28.02.2010 bis 31.12.2011, Finanzierung durch José, Costa Rica, 01.10.2011 bis 30.09.2015, Finanzierung durch DAAD-Stipendium (Dr. U. Scholz, M. Lange/Arbeitsgruppe Bio- DAAD-Stipendium (Dr. W. Weschke/Arbeitsgruppe Samenent- informatik und Informationstechnologie). wicklung).

217 Arbeitsaufenthalte von Gästen im IPK/Guest Researchers at the IPK

Dr. Yaroslav Pirko, National Academy of Science, Institute of Dr. Michael Wybrow, Monash University, Melbourne, Austra- Food Biotechnology and Genomics, Department of Genomics lien, 19.08.2010 bis 27.08.2010, Finanzierung durch Monash and Molecular Biotechnology, Kiew, Ukraine, 03.07.2011 bis Universitiy (Prof. Dr. F. Schreiber/Arbeitsgruppe Pflanzenbioin- 30.09.2012, Finanzierung durch DFG (Dr. W. Weschke/Arbeits- formatik). gruppe Samenentwicklung). Yasdan Asgari, University of Tehran, Institute of Biochemis- Maria Mildner, 01.10.2010 bis 31.10.2010, Eigenfinanzierung try and Biophysics, Tehran, Iran, 22.06.2011 bis 21.08.2011, Fi- (Dr. H. Bäumlein/Arbeitsgruppe Genregulation). nanzierung durch University of Tehran (Prof. Dr. F. Schreiber/ Arbeitsgruppe Pflanzenbioinformatik). Dr. Amal Joseph Johnston, Eidgenössische Technische Hoch- schule Zürich, Abteilung Biologie, Zürich, Schweiz, 01.09.2010 Dr. Kambiz Baghalian, University of Agriculture Tokyo, Tokyo, bis 31.08.2012, Finanzierung durch Stipendium der A. von Japan, 03.04.2011 bis 30.11.2011, Eigenfinanzierung (Prof. Dr. F. Humboldt-Stiftung (Dr. H. Bäumlein/Arbeitsgruppe Genregu- Schreiber/Arbeitsgruppe Pflanzenbioinformatik). lation). Dr. Christiane Seiler, 01.11.2010 bis 30.04.2011, Eigenfinanzie- Diep Le Hong, Vietnamese Academy of Science and Technol- rung (Dr. N. Sreenivasulu/Arbeitsgruppe Stress-Genomik). ogy, Institute of Biotechnology, Hanoi, Vietnam, 01.01.2010 bis 31.03.2010 und 01.10.2010 bis 14.03.2011, Finanzierung durch Dr. Marc Strickert, Universität Siegen, Fakultät Medieninfor- Ministry of Education & Training/Vietnam (Dr. H. Bäumlein/Ar- matik, 01.11.2010 bis 30.09.2012, Eigenfinanzierung (Dr. N. beitsgruppe Genregulation). Sreenivasulu/Arbeitsgruppe Stress-Genomik).

Anna Schallau, 11.08.2010 bis 28.02.2011, Eigenfinanzierung Dr. Sudhakar Reddy Palakolanu, 06.11.2011 bis 05.11.2012, und Finanzierung durch IPK (Dr. H. Bäumlein/Arbeitsgruppe Finanzierung durch Leibniz-DAAD-Stipendium (Dr. N. Sreeniva- Genregulation). sulu/Arbeitsgruppe Stress-Genomik).

Petra Sarahnova, Palacky University, Department of Bota- ny, Tschechische Republik, 28.10.2010 bis 07.02.2011 und 04.10.2011 bis 31.03.2012, Finanzierung durch DAAD-Stipendi- Abteilung Physiologie und Zellbiologie um (Dr. H. Bäumlein/Arbeitsgruppe Genregulation). Nunun Barunawati, Brawijaya University, Malang, Indonesien, Isabella Herrmann, Martin-Luther-Universität Halle-Witten- 01.01.2010 bis 30.06.2012, Finanzierung durch indonesische berg, Halle/S., 01.01.2010 bis 30.06.2010, Eigenfinanzierung Regierung (Prof. Dr. N. von Wirén/Arbeitsgruppe Molekulare (Dr. U. Conrad/Arbeitsgruppe Phytoantikörper). Pflanzenernährung).

Phan Trong Hoang, University of Science, Institute of Biotech- Dmitriy Fedoseyenko, Universität Hohenheim, Stuttgart- nology, Hanoi, Vietnam, 01.01.2010 bis 29.02.2012, Finanzierung Hohenheim, 01.01.2010 bis 31.12.2010 und 01.07.2011 bis durch Stipendium vom Ministry of Education and Training, Viet- 31.07.2011, Eigenfinanzierung (Prof. Dr. N. von Wirén/Arbeits- nam (Dr. U. Conrad/Arbeitsgruppe Phytoantikörper). gruppe Molekulare Pflanzenernährung).

Dr. Nguyen Huu Cuong, Institute of Biotechnology, Hanoi, Mariela Monteoliva, University of Córdoba, Department of Vietnam, 08.08.2010 bis 04.10.2010, Finanzierung durch DAAD Biological Chemistry, Córdoba, Argentinien, 14.11.2010 bis (Dr. U. Conrad/Arbeitsgruppe Phytoantikörper). 20.12.2010, Finanzierung durch BMBF (Prof. Dr. N. von Wirén/ Arbeitsgruppe Molekulare Pflanzenernährung). Alejandro Sarriòn Perdigones, Instituto des Biologia Mole- cular y Celular de Plantas, Valencia, Spanien, 17.09.2011 bis Amirhossein Ahkami, Universität Hohenheim, Stuttgart-Ho- 19.11.2011, Finanzierung durch Ministerium für Wissenschaft henheim, 01.04.2010 bis 30.09.2010, Finanzierung über Univer- und Innovation, Spanien (Dr. U. Conrad/Arbeitsgruppe Phy- sität Hohenheim (Prof. Dr. N. von Wirén/Arbeitsgruppe Moleku- toantikörper). lare Pflanzenernährung).

Dr. Urs Hähnel, Julius Kühn-Institut, Quedlinburg, 01.01.2010 Ricardo Fabiano Hettwer Giehl, Universität Hohenheim, bis 16.05.2010, Finanzierung durch Julius Kühn-Institut (Dr. L. Stuttgart-Hohenheim, 03.05.2010 bis 31.03.2011, Finanzierung Altschmied/Arbeitsgruppe Expressionskartierung). durch Brasilianisches Bildungsministerium ‑ CAPES (Prof. Dr. N. von Wirén/Arbeitsgruppe Molekulare Pflanzenernährung). Prof. Ali Masoudi-Nejad, Universität Teheran, Teheran, Iran, 01.08.2010 bis 10.09.2010, Finanzierung durch DAAD-Stipendi- um (Prof. Dr. F. Schreiber/Arbeitsgruppe Pflanzenbioinformatik).

218 Zeynep Isik, Universität Hohenheim, Stuttgart-Hohenheim, Dr. Vanesa Tognetti, Ghent University, VIB Department of Plant 18.03.2010 bis 26.03.2010, Finanzierung durch Stipendium der Systems Biology, Gent, Belgien, 12.05.2011 bis 17.05.2011, Fi- Universität Hohenheim (Prof. Dr. N. von Wirén/Arbeitsgruppe nanzierung durch IPK (Dr. M. Hajirezaei/Arbeitsgruppe Moleku- Molekulare Pflanzenernährung). lare Pflanzenernährung).

Fengying Duan, China Agricultural University, Beijing, China, Yanelis Karina Capdesuner Ruiz, Institute Centro de Bio- 01.11.2010 bis 31.10.2012, Finanzierung durch chinesische plantas, Ciego de Avila, Cuba, 01.01.2010 bis 29.08.2010 und Regierung (Prof. Dr. N. von Wirén/Arbeitsgruppe Molekulare 16.06.2011 bis 10.12.2011, Finanzierung durch DAAD-Stipendi- Pflanzenernährung). um (Dr. H.-P. Mock/Arbeitsgruppe Angewandte Biochemie).

Baris Boylu, Middle East Technical University, Ankara, Türkei, Jan Fila, Institute of Experimental Botany, Prag, Tschechische 26.08.2011 bis 31.08.2011, Eigenfinanzierung (Prof. Dr. N. von Republik, 02.03.2010 bis 28.03.2010, Finanzierung durch EU- Wirén/Arbeitsgruppe Molekulare Pflanzenernährung). COST; 14.11.2010 bis 27.11.2010, Eigenfinanzierung; 31.07.2011 bis 18.09.2011, Finanzierung durch DAAD (Dr. H.-P. Mock/Ar- Michiko Takahashi, Utsunomiya University, Utsunomiya, Ja- beitsgruppe Angewandte Biochemie). pan, 22.04.2011 bis 21.09.2011, Eigenfinanzierung (Prof. Dr. N. von Wirén/Arbeitsgruppe Molekulare Pflanzenernährung). Mohsen Janmohammadi, University of Tehran, Department of Agronomy and Plant Breeding, Teheran, Iran, 01.01.2010 bis Prof. Lixing Yuan, Chinese Agricultural University, Beijing, 22.04.2010, Finanzierung durch Forschungsstipendium der Re- China, 12.08.2011 bis 02.09.2011, Finanzierung durch Robert- publik Iran (Dr. H.-P. Mock/Arbeitsgruppe Angewandte Bioche- Bosch-Foundation (Prof. Dr. N. von Wirén/Arbeitsgruppe Mole- mie). kulare Pflanzenernährung). Manuela Eick, Institut für Botanik und Landschaftsökologie Susanne Schmidt, University of Queensland, School of Agri- EMAU, Greifswald, 18.07.2010 bis 30.07.2010, Eigenfinanzie- culture & Food Science, Queensland, Australien, 14.05.2011 bis rung (Dr. A. Matros/Arbeitsgruppe Angewandte Biochemie). 30.06.2011, Finanzierung durch Humboldt-Stipendium (Prof. Dr. N. von Wirén/Arbeitsgruppe Molekulare Pflanzenernährung). Giuseppe Lattanzio, Estación Experimental de Aula Dei, Dpto. Nutrición Vegetal, Italien, 01.08.2010 bis 22.11.2010, Eigenfi- Majid Ghorbani Javid, Tarbiar Modares University, Department nanzierung (Dr. H.-P. Mock/Arbeitsgruppe Angewandte Bio- of Agronomy, Agriculture Faculty, Teheran, Iran, 11.03.2010 bis chemie). 10.09.2010, Finanzierung durch Ministry of Science, Research and Technology in Teheran (Dr. M. Hajirezaei/Arbeitsgruppe Dr. Katja Witzel, Universität Kiel, Fakultät für Agrar- und Ernäh- Molekulare Pflanzenernährung). rungswissenschaften, 08.11.2010 bis 26.11.2010; 16.05.2011 bis 20.05.2011 und 01.11.2011 bis 08.11.2011, Eigenfinanzierung Dr. Mathias Zurbriggen, Universidad de Rosario, Rosario, (Dr. H.-P. Mock/Arbeitsgruppe Angewandte Biochemie). Argentinien, 21.06.2010 bis 30.06.2010 und 29.08.2010 bis 23.09.2010, Finanzierung durch DFG (Dr. M. Hajirezaei/Arbeits- Dr. Elisabetta Mazzucotelli, Genomic Research Centre, Fioren- gruppe Molekulare Pflanzenernährung). zuola d´Arda, Italien, 18.04.2010 bis 28.05.2010, Finanzierung durch EU COST Action (Dr. H.-P. Mock/Arbeitsgruppe Ange- Dr. Nestor Carillo, Universidad de Rosario, Rosario, Argen- wandte Biochemie). tinien, 09.09.2010 bis 23.09.2010, Finanzierung durch DFG; 12.05.2011 bis 17.05.2011, Finanzierung durch IPK (Dr. M. Haji- José Àngel Huerta Ocampo, Potosi Institute of Scientific Re- rezaei/Arbeitsgruppe Molekulare Pflanzenernährung). search and Technology, San Luis Potosi, Mexico, 04.04.2011 bis 30.11.2011, Eigenfinanzierung (Dr. H.-P. Mock/Arbeitsgruppe Maria Alvarez, Universidad Nacional de Córdoba, Córdoba, Angewandte Biochemie). Argentinien, 11.09.2010 bis 21.09.2010, Finanzierung durch MINCYT ‑ BMBF (Dr. M. Hajirezaei/Arbeitsgruppe Molekulare Amenehsadat Hashemi, Agricultural Biotechnology Research Pflanzenernährung). Institute of Iran (ABRII), Department of Physiology and Pro- teomics, Karaj, Iran, 19.06.2011 bis 18.12.2011, Finanzierung Young-Min Kim, 01.02.2011 bis 31.01.2012, Eigenfinanzierung durch Ministry of Science, Research and Technology in Teheran (Dr. M. Hajirezaei/Arbeitsgruppe Molekulare Pflanzenernäh- (Dr. H.-P. Mock/Arbeitsgruppe Angewandte Biochemie). rung). Sunita Yadav, University of Delhi, Department of Botany, New Medhi Ghaboli, Agricultural Biotechnology Research Institute Delhi, Indien, 01.09.2011 bis 30.11.2011, Finanzierung durch of Iran (ABRII), Karaj, Iran, 05.08.2011 bis 04.04.2012, Finanzie- DAAD-Fellowship (Dr. H.-P. Mock/Arbeitsgruppe Angewandte rung durch Ministry of Science and Technology of Iran (Dr. M. Biochemie). Hajirezaei/Arbeitsgruppe Molekulare Pflanzenernährung).

219 Arbeitsaufenthalte von Gästen im IPK/Guest Researchers at the IPK

Marek Marzec, University of Silesia, Katowice, Polen, 01.01.2010 Katarzyna Plasun, 01.01.2011 bis 31.03.2012, Eigenfinanzie- bis 29.03.2010, Finanzierung durch UPGOW-Europäische Uni- rung (Dr. J. Kumlehn/Arbeitsgruppe Pflanzliche Reproduktions- on; 01.04.2011 bis 21.04.2011, 02.06.2011 bis 28.06.2011 und biologie). 29.09.2011 bis 20.10.2011, Finanzierung durch DAAD (Dr. M. Melzer/Arbeitsgruppe Strukturelle Zellbiologie). Inés Cambra, University of Madrid, Madrid, Spanien, 11.04.2011 bis 14.07.2011, Finanzierung durch University of Madrid; Dr. Ahmed Eisa Mahmoud Ghoniem, University of Cairo, Bota- 15.08.2011 bis 14.09.2011, Finanzierung durch IPK (Dr. J. Kum- ny Department, Agriculture Faculty, Kairo, Ägypten, 10.10.2010 lehn/Arbeitsgruppe Pflanzliche Reproduktionsbiologie). bis 09.11.2010, Finanzierung durch Universität Kairo (Dr. M. Melzer/Arbeitsgruppe Strukturelle Zellbiologie). Katarzyna Szyrajew, University of Silesia, Katowice, Polen, 19.09.2011 bis 18.12.2011, Finanzierung durch ERASMUS-Sti- Pooja Pandey, Integral University of Lucknow, Indien, pendium (Dr. J. Kumlehn/Arbeitsgruppe Pflanzliche Reproduk- 24.09.2011 bis 30.09.2011, Finanzierung durch DAAD-Siemens- tionsbiologie). Stipendium (Dr. M. Melzer/Arbeitsgruppe Strukturelle Zellbio- logie). Monika Gajecka, University of Silesia, Department of Genetics, Katowice, Polen, 06.06.2011 bis 05.09.2011, Finanzierung durch Prof. Louise Andersen, University of Illinois, Department of ERASMUS-Stipendium (Dr. J. Kumlehn/Arbeitsgruppe Pflanzli- Biological Sciences, Chicago, USA, 28.04.2011 bis 17.05.2011, che Reproduktionsbiologie). Eigenfinanzierung (Dr. M. Melzer/Arbeitsgruppe Strukturelle Zellbiologie). Stefan Hiekel, Friedrich-Schiller-Universität, Jena, 10.10.2011 bis 30.11.2011, Finanzierung durch IPK (Dr. J. Kumlehn/Arbeits- Stefanie Goedeke 01.04.2010 bis 31.12.2010, Eigenfinanzie- gruppe Pflanzliche Reproduktionsbiologie). rung (Dr. J. Kumlehn/Arbeitsgruppe Pflanzliche Reproduktions- biologie). Dr. Federica Bini, 01.09.2011 bis 28.02.2012, Eigenfinanzie- rung (Dr. J. Kumlehn/Arbeitsgruppe Pflanzliche Reproduktions- Malik Zahid Abbas, Wheat Biotechnology Laboratory, Plant biologie). Transformation Group Agricultural Biotechnology Division, NIBGE, Faisalabad, Pakistan, 17.06.2010 bis 16.12.2010, Finan- Dr. Francesca Tedeschi, University of Tuscia, Viterbo, Italien, zierung über Stipendium der Higher Education Commision (Dr. 01.10.2010 bis 30.09.2011, Finanzierung durch University of Tus- J. Kumlehn/Arbeitsgruppe Pflanzliche Reproduktionsbiologie). cia (Dr. J. Kumlehn/Arbeitsgruppe Pflanzliche Reproduktions biologie).

220 Dr. Mohammad Pourkheirandish, National Institute of Agro- Mauricio Toro Nahuelpan, University of La Serena, La Serena, biological Sciences (NIAS), Tsukuba, Japan, 03.01.2011 bis Chile, 19.08.2010 bis 03.03.2011, Finanzierung durch Stipendi- 08.01.2011, Finanzierung durch NIAS (Dr. J. Kumlehn/Arbeits- um der Bayer-Stiftung (Prof. Dr. G. Kunze/Arbeitsgruppe Hefe- gruppe Pflanzliche Reproduktionsbiologie). genetik).

Dr. Prasanthi Perera, International Center for Tropical Agricul- Ravi Kumar Yelchuri, Institute of Microbial Technology, Council ture (CIAT), Cali, Colombia, 23.09.2011 bis 23.10.2011, Finan- of Scientific and Industrial Research Sector, Indien, 16.11.2010 zierung durch CIAT (Dr. J. Kumlehn/Arbeitsgruppe Pflanzliche bis 10.12.2010, Finanzierung durch BMBF (Prof. Dr. G. Kunze/ Reproduktionsbiologie). Arbeitsgruppe Hefegenetik).

Miguel Alvaro Benito, Universidad Autonoma de Madrid, Dr. Burla Sashidar, The Energy and Resources Institute (TERI), Centro de Biología Molecular „Severo Ochoa“, Madrid, Spanien, New Delhi, Indien, 08.04.2010 bis 28.06.2010, Finanzierung 23.08.2010 bis 26.11.2010, Finanzierung durch CBMSO/Univer- durch DLR (Prof. Dr. G. Kunze/Arbeitsgruppe Hefegenetik). sidad Autonoma de Madrid (Prof. Dr. G. Kunze/Arbeitsgruppe Hefegenetik). Prof. Keith Baronian, University of Canterbury, School of Biological Sciences, Christchurch, Neuseeland, 17.06.2010 Thi Minh Ha Pham, Institute of Biotechnology, Vietnam Aca- bis 28.06.2010, Eigenfinanzierung (Prof. Dr. G. Kunze/Arbeits- demy of Science and Technology, Hanoi, Vietnam, 01.01.2010 gruppe Hefegenetik). bis 31.07.2011, Finanzierung durch MOET-Stipendium Vietnam (Prof. Dr. G. Kunze/Arbeitsgruppe Hefegenetik). Dr. Viviana Ordenes, CEAZA Campus Andreas Bello, Chile, 11.10.2010 bis 25.10.2010, Eigenfinanzierung (Prof. Dr. G. Kun- Kinga Sedzielewska, AMykor GmbH, 01.01.2010 bis 14.09.2010, ze/Arbeitsgruppe Hefegenetik). Finanzierung durch Kooperationsvertrag mit AMykor GmbH (Prof. Dr. G. Kunze/Arbeitsgruppe Hefegenetik). Sabine Nutz, Julius Kühn-Institut, Quedlinburg, 01.02.2011 bis 04.03.2011, Finanzierung durch IPK (Prof. Dr. G. Kunze/Arbeits- Tabias Samuel Lemanski, Wrocław University of Technology, gruppe Hefegenetik). Wrocław, Polen, 08.02.2010 bis 07.05.2010, Finanzierung durch ComEAST (Prof. Dr. G. Kunze/Arbeitsgruppe Hefegenetik).

221 Arbeitsaufenthalte von IPK-Wissenschaftlern in anderen Einrichtungen/Stays of IPK Researchers at other Institutions

Arbeitsaufenthalte von IPK-Wissenschaftlern in anderen Einrichtungen/ Stays of IPK Researchers at Other Institutions

Abteilung Genbank Abteilung Cytogenetik und Genomanalyse

Tina Seidensticker/Arbeitsgruppe Pflanzliche Baupläne, Dr. Gabriele Jovtchev/Arbeitsgruppe Karyotypevolution, Università degli Studi di Milano, Lodi, Italien, 21.06.2010 bis Institute of Biophysics, Department of Plant Developmental 10.07.2010, Finanzierung durch BMBF. Genetics, Brno, Tschechische Republik, 26.07.2010 bis 30.07.2010, Finanzierung durch Projekt des Landes Sachsen- Piotr Gawroński/Arbeitsgruppe Pflanzliche Baupläne, Univer- Anhalt. sity of Liverpool, Liverpool, UK, 31.03.2011 bis 16.04.2011, Fi- nanzierung durch IPK. Dr. Andreas Houben/Arbeitsgruppe Chromosomenstruktur und -funktion, Chinese Academy of Sciences, Institute of Ge- Manuela Nagel/Arbeitsgruppe Ressourcengenetik und Re- netics, Peking, China, 06.11.2011 bis 20.11.2011, Finanzierung produktion, Seed Conservation Department, Millennium Seed durch Chinese Academy of Sciences. Bank, Kew Gardens, Großbritannien, 13.03.2010 bis 08.05.2010 und 08.11.2010 bis 24.11.2010, Finanzierung durch IPK und Stefan Heckmann/Arbeitsgruppe Chromosomenstruktur und Millennium Seed Bank. -funktion, Jammu University, Jammu, Indien, 12.03.2011 bis 24.03.2011, Finanzierung durch DAAD. Dr. Frank Blattner/Arbeitsgruppe Experimentelle Taxonomie, Department of Botany, Mongolian National University, Ulaan Jeyaraman Rajaraman/Arbeitsgruppe Transkriptomanalyse, Baator, Mongolei, 03.08.2010 bis 01.09.2010, Finanzierung Technische Universität München, München, 25.04.2010 bis durch DFG. 08.05.2010, Finanzierung durch IPK.

Dr. Frank Blattner und Dr. Sabine Jakob/Arbeitsgruppe Jemanesh Kifetew Haile/Arbeitsgruppe Gen- und Genomkar- Experimentelle Taxonomie, Feldarbeit, Südost-Argentinien, tierung, Institute for Maize and Wheat Improvement Centre, 15.01.2011 bis 17.02.2011, Finanzierung durch DFG. CIMMYT, Mexiko, 05.03.2011 bis 29.03.2011, Finanzierung durch „Jeanie Borlaug Women in Triticum Award 2010“. Dr. Frank Blattner/Arbeitsgruppe Experimentelle Taxonomie, Feldarbeit, Kalifornien, USA, 01.06.2011 bis 16.06.2011, Finan- zierung durch DFG.

Dr. Reinhard Fritsch/Arbeitsgruppe Taxonomie pflanzengenetischer Ressourcen, Iranian Research Institute of Plant Pro- tection, Teheran, Iran, 24.04.2010 bis 15.05.2010, Finanzierung durch DFG.

Dr. Klaus Pistrick/Arbeitsgruppe Taxonomie pflanzengenetischer Ressourcen, Institut für Botanik und Botanischer Gar- ten, Tbilisi, Georgien, 20.07.2010 bis 04.08.2010, Finanzierung durch IPK.

Nikolai Matthias Nürk/Arbeitsgruppe Experimentelle Ta- xonomie, University Idaho, Stillinger Herbarium, Idaho, USA, 05.11.2010 bis 28.11.2010, Finanzierung durch DFG.

Dr. Sabine Jakob/Arbeitsgruppe Experimentelle Taxonomie, Dushanbe, Tadschikistan, 18.06.2010 bis 10.07.2010, Finanzie- rung durch DFG.

222 Abteilung Molekulare Genetik Abteilung Physiologie und Zellbiologie

Gerd Melkus/Arbeitsgruppe Heterosis, University Park, Park, Dr. Mohammad-Reza Hajirezaei/Arbeitsgruppe Molekulare USA, 12.04.2010 bis 01.05.2010, Finanzierung durch BMBF (GABI- Pflanzenernährung, Institute of Molecular and Cell Biology, SysSEED). Department of Physiology and Cell Biology, Rosario, Argen- tinien, 21.02.2010 bis 25.02.2010, Finanzierung durch DLR und Dr. Ljoudmilla Borisjuk/Arbeitsgruppe Heterosis, Penn State MinCyt/BMBF. University, Pennsylvania, USA, 20.12.2010 bis 09.01.2011 und 31.05.2011 bis 20.06.2011, Finanzierung durch Industriepro- Dr. Michael Melzer/Arbeitsgruppe Strukturelle Zellbiologie, jekt. Molecular Biology and Agriculture Division, Bhaba Atomic Research Center, Mumbai, Indien, 04.03.2010 bis 11.04.2010, Olga Kirioukhova/Arbeitsgruppe Genregulation, National Finanzierung durch DLR/BMBF. Institute for Basic Biology, Obazaki, Japan, 19.11.2010 bis 01.01.2011, Finanzierung durch Humboldt-Stipendium. Dr. Michael Melzer/Arbeitsgruppe Strukturelle Zellbiologie, Department of Genetics, Faculty of Biology and Environment Valeska Hauptmann/Arbeitsgruppe Phytoantikörper, INRA, Protection, University of Silesia, Katowice, Polen, 17.05.2011 bis Nantes, Frankreich, 15.03.2010 bis 30.04.2010, Finanzierung 22.05.2011, Finanzierung durch DAAD. durch COST Action der Europäischen Union. Dr. Twan Rutten/Arbeitsgruppe Strukturelle Zellbiologie, Prof. Dr. Falk Schreiber/Arbeitsgruppe Pflanzenbioinformatik, Department of Genetics, Faculty of Biology and Environment University of Sydney; ACPFG Adelaide und University of South Protection, University of Silesia, Katowice, Polen, 28.11.2011 bis Australia; University of Newcastle; Monash University Mel- 02.12.2011, Finanzierung durch DAAD. bourne und DSTO Melbourne; NICTA Sydney; University Western Australia Perth; CSIRO Canberra, Australien, 11.01.2010 Prof. Dr. Gotthard Kunze/Arbeitsgruppe Hefegenetik, Univer- bis 15.02.2010, teilweise Finanzierung durch University of South sity of Canterbury, School of Biological Sciences, Canterbury, Australia und University Western Australia Perth. Neuseeland, 18.11.2010 bis 25.11.2010, Finanzierung durch In- dustrieprojekt mit Neuseeland. Prof. Dr. Falk Schreiber/Arbeitsgruppe Pflanzenbioinformatik, University of Newcastle; CSIRO Canberra; University of Sydney Dagmara Jankowska/Arbeitsgruppe Hefegenetik, Institute und NICTA Sydney; Monash University Melbourne; University of Microbial Technology, Chandigarh, Indien, 13.12.2010 bis Western Australia Perth, Australien, 21.11.2010 bis 13.12.2010, 17.12.2010, Finanzierung durch DAAD. teilweise Finanzierung durch CSIRO Canberra und University Western Australia Perth. Anke Trautwein/Arbeitsgruppe Hefegenetik, Institute of Micro- bial Technology, Chandigarh, Indien, 13.12.2010 bis 17.12.2010, Dr. Christian Klukas/Arbeitsgruppe Bildanalyse, Zhejiang Uni- Finanzierung durch DAAD. versity, Department of Bioinformatics, College of Life Sciences, Hangzhou, China, 22.07.2010 bis 04.08.2010 und 17.07.2011 bis 29.07.2011, Finanzierung durch Research Fund for International Young Scientists, NSFC of China.

Dr. Nese Sreenivasulu/Arbeitsgruppe Stress-Genomik, Na- tional Institute of Plant Genome Research, New Delhi; Univer- sity of Agricultural Sciences, Department of Crop Physiology, Bangalore, Indien, 30.11.2010 bis 31.01.2011, Finanzierung durch BMBF.

223 Lehrtätigkeit/Teaching

Lehrtätigkeit/Teaching

2010

Name der/des Lehrenden Thema Universität/ Fakultät/ SWS* Hochschule Fachbereich (FB) Prof. Dr. A. Graner (GB) Masterstudiengang Nutz Martin-Luther- Naturwissenschaftliche 4 Priv.-Doz. Dr. A. Börner (GB) pflanzenwissenschaften, Universität Halle- Fakultät III Dr. N. Stein (GB) Mastermodul: Pflanzengenetische Wittenberg Institut für Agrar- und Dr. B. Kilian (GB) Ressourcen und Genomforschung Ernährungswissenschaften (Vorlesung und Praktikum) Priv.-Doz. Dr. A. Börner (GB) Erhaltungsstrategien und Martin-Luther- Naturwissenschaftliche 1 Dr. J. Keller (GB) Management pflanzengenetischer Universität Halle- Fakultät III Dr. U. Lohwasser (GB) Ressourcen Wittenberg Institut für Agrar- und Dr. A. Senula (GB) (Vorlesung und Praktikum) Ernährungswissenschaften Dr. A. Senula (GB) Kryokonservierung von Minze- Martin-Luther- Naturwissenschaftliche 10 Akzessionen (Mentha) Universität Halle- Fakultät I (Praktikum) Wittenberg Institut für Pharmazie Dr. T. Schnurbusch (GB) Quantitative Genetik und Martin-Luther- Naturwissenschaftliche 2 Populationsgenetik in der Universität Halle- Fakultät III Pflanzenzüchtung Wittenberg Institut für Agrar- und (Vorlesung) Ernährungswissenschaften Prof. Dr. I. Schubert (CYG) Klassische und molekulare Universität Kassel Fachbereich Genetik 7 Dr. J. Fuchs (CYG) Cytogenetik (Komplexpraktikum) Priv.-Doz. Dr. V. Schubert (CYG) Moderne Techniken der Martin-Luther- Naturwissenschaftliche 1 Dr. J. Fuchs (CYG) Mikroskopie und Cytogenetik Universität Halle- Fakultät III Dr. M. Melzer (PZB) (Bachelor-Studentenpraktikum) Wittenberg Institut für Agrar- und Ernährungswissenschaften Dr. A. Houben (CYG) Biotechnologie in der Hochschule Anhalt FB Landwirtschaft 7 Dr. H. Bäumlein (MOG) Pflanzenproduktion Bernburg (Vorlesung und Praktikum) Dr. A. Houben (CYG) Zytogenetik und Gentechnologie Martin-Luther- Naturwissenschaftliche 1 Priv.-Doz. Dr. V. Schubert (CYG) der Nutzpflanzen Universität Halle- Fakultät III Dr. J. Kumlehn (PZB) (Praktikum) Wittenberg Institut für Agrar- und Ernährungswissenschaften Priv.-Doz. Dr. R. Schmidt (CYG) Einblicke durch Genomprojekte: Universität Potsdam Mathematisch-Natur 2 Was machen Pflanzen anders? wissenschaftliche Fakultät (Seminar) Priv.-Doz. Dr. R. Schmidt (CYG) Struktur-/Funktionsbeziehungen Universität Potsdam Mathematisch-Natur 2 in Eukaryontengenomen wissenschaftliche Fakultät (Seminar) Prof. Dr. A.M. Wobus (CYG) Grundlagen der Zell- und Martin-Luther- Medizinische Fakultät 1 Gewebekultur und aktuelle Universität Halle- Aspekte der Stammzellforschung Wittenberg (Vorlesung) Dr. U. Scholz (CYG) Einführung in die Bioinformatik Hochschule Anhalt FB Biotechnologie, 4 Dr. M. Lange (CYG) (Vorlesung und Übung) Köthen Lebensmitteltechnologie, Dr. S. Weise (CYG) Verfahrens- und Umwelttechnik Dr. M. Lange (CYG) Datenbankmanagementsysteme Hochschule Harz FB Automatisierung und 6 Dr. S. Weise (CYG) Medieninformatik Wernigerode Informatik Bachelor-Studiengang (Vorlesung und Übung)

* SWS = Semesterwochenstunden

224 Name der/des Lehrenden Thema Universität/ Fakultät/ SWS* Hochschule Fachbereich (FB) Prof. Dr. T. Altmann (MOG) Projektmodul Molekulare Genetik Martin-Luther- Naturwissenschaftliche 15 Dr. R.C. Meyer (MOG) (Vorlesung) Universität Halle- Fakultät I Dr. W. Weschke (MOG) Wittenberg Institut für Biologie Dr. H. Weber (MOG) Dr. U. Conrad (MOG) Dr. habil. L. Altschmied (MOG) Dr. S. Friedel (MOG) Prof. Dr. T. Altmann (MOG) Biologie der Samenentwicklung Universität Hannover FB Pflanzenproteomik 6 Dr. H. Rolletschek (MOG) (Praktikum) Institut für Pflanzengenetik Dr. U. Conrad (MOG) Grundlagen der Genetik Martin-Luther- Naturwissenschaftliche 1 (Vorlesung) Universität Halle- Fakultät I Wittenberg FB Pharmazie Dr. U. Conrad (MOG) Molecular Pharming im Masterkurs Martin-Luther- Naturwissenschaftliche 1 Pharmazeutische Biotechnologie Universität Halle- Fakultät I (Vorlesung) Wittenberg FB Pharmazie Dr. habil. L. Altschmied (MOG) Pflanzenphysiologisches Friedrich-Schiller- Institut für Pflanzen 4 Praktikum Universität Jena physiologie Prof. Dr. F. Schreiber (MOG) Analyse biologischer Netzwerke Martin-Luther- Naturwissenschaftliche 4 H. Mehlhorn (MOG) (Vorlesung und Übung) Universität Halle- Fakultät III Wittenberg Institut für Informatik Prof. Dr. F. Schreiber (MOG) Bioinformatik-Intensivkurs Christian-Albrechts- Agrar- und Ernährungs 4 Dr. U. Scholz (CYG) Master-Studiengang Universität zu Kiel wissenschaftliche Fakultät Dr. S. Friedel (MOG) Agrarwissenschaften Dr. C. Klukas (MOG) (Vorlesung und Übung) Dr. B. Junker (PZB) Dr. H. Knüpffer (GB) Dr. N. Sreenivasulu (MOG) Molecular basis of terminal University of Western Summer school of plant 1 Senior Lecturer drought tolerance Australia, Perth biology (Vorlesung) Prof. Dr. N. von Wirén (PZB) Pflanzenphysiologie: Martin-Luther- Naturwissenschaftliche 2 Transportprozesse über Universität Halle- Fakultät I pflanzliche Membranen; Wittenberg Institut für Biologie Molekularer Mineralstoffwechsel Priv.-Doz. Dr. H.-P. Mock (PZB) Pflanzenphysiologie Martin-Luther- Naturwissenschaftliche 4 (Grundpraktikum) Universität Halle- Fakultät I Wittenberg Institut für Biologie Prof. Dr. G. Kunze (PZB) Molekulargenetik Teil I Hochschule Anhalt FB Biotechnologie, 3 (Vorlesung) Köthen Lebensmitteltechnologie, Verfahrens- und Umwelttechnik Prof. Dr. G. Kunze (PZB) Molekulargenetik Teil II Hochschule Anhalt FB Biotechnologie, 3 (Vorlesung) Köthen Lebensmitteltechnologie, Verfahrens- und Umwelttechnik Prof. Dr. G. Kunze (PZB) Biosensoren für die Hochschule Anhalt FB Biotechnologie, 2 Umweltkontrolle (Vorlesung) Köthen Lebensmitteltechnologie, Verfahrens- und Umwelttechnik Prof. Dr. G. Kunze (PZB) Hefegenetik Ernst-Moritz-Arndt- Mathematisch-Natur 4 (Praktikum) Universität Greifswald wissenschaftliche Fakultät, FB Biologie Institut für Genetik und Biochemie Semesterwochenstunden (SWS) insgesamt: 102

* SWS = Semesterwochenstunden

225 Lehrtätigkeit/Teaching

2011

Name der/des Lehrenden Thema Universität/ Fakultät/ SWS* Hochschule Fachbereich (FB) Prof. Dr. A. Graner (GB) Masterstudiengang Nutz Martin-Luther- Naturwissenschaftliche 4 Priv.-Doz. Dr. A. Börner (GB) pflanzenwissenschaften Universität Halle- Fakultät III Dr. N. Stein (GB) Mastermodul: Pflanzengenetische Wittenberg Institut für Agrar- und Dr. B. Kilian (GB) Ressourcen und Genomforschung Ernährungswissenschaften (Vorlesung und Praktikum) Priv.-Doz. Dr. A. Börner (GB) Erhaltungsstrategien und Martin-Luther- Naturwissenschaftliche 1 Dr. J. Keller (GB) Management pflanzengenetischer Universität Halle- Fakultät III Dr. U. Lohwasser (GB) Ressourcen Wittenberg Institut für Agrar- und Dr. A. Senula (GB) (Vorlesung und Praktikum) Ernährungswissenschaften Dr. T. Schnurbusch (GB) Quantitative Genetik und Martin-Luther- Naturwissenschaftliche 2 Populationsgenetik in der Universität Halle- Fakultät III Pflanzenzüchtung Wittenberg Institut für Agrar- und (Vorlesung) Ernährungswissenschaften Dr. J. Keller (GB) Cryopreservation and in vitro Martin-Luther- Biozentrum der Universität 1,5 storage for plant germplasm Universität Halle- Halle conservation (Vorlesung) Wittenberg Prof. Dr. I. Schubert (CYG) Klassische und molekulare Universität Kassel Fachbereich Genetik 7 Dr. J. Fuchs (CYG) Cytogenetik (Komplexpraktikum) Priv.-Doz. Dr. V. Schubert (CYG) Moderne Techniken der Martin-Luther- Naturwissenschaftliche 0,5 Dr. J. Fuchs (CYG) Mikroskopie und Cytogenetik Universität Halle- Fakultät III Dr. M. Melzer (PZB) (Bachelor-Studentenpraktikum) Wittenberg Institut für Agrar- und Ernährungswissenschaften Priv.-Doz. Dr. V. Schubert (CYG) Grundlagen und moderne Martin-Luther- Naturwissenschaftliche 1,5 Techniken der Mikroskopie im Universität Halle- Fakultät III Masterstudiengang „Nutzpflanzen Wittenberg Institut für Agrar- und wissenschaften“ Ernährungswissenschaften (Vorlesung und Praktikum) Dr. A. Houben (CYG) Biotechnologie in der Pflanzen- Hochschule Anhalt FB Landwirtschaft 7 Dr. H. Bäumlein (MOG) und Tierproduktion Bernburg (Vorlesung und Praktikum) Priv.-Doz. Dr. R. Schmidt (CYG) Einblicke durch Genomprojekte: Universität Potsdam Mathematisch-Natur 2 Was machen Pflanzen anders? wissenschaftliche Fakultät (Seminar) Priv.-Doz. Dr. R. Schmidt (CYG) Struktur-/Funktionsbeziehungen Universität Potsdam Mathematisch-Natur 2 in Eukaryontengenomen wissenschaftliche Fakultät (Seminar) Prof. Dr. A.M. Wobus (CYG) Grundlagen der Zell- und Martin-Luther- Medizinische Fakultät 1 Gewebekultur und aktuelle Universität Halle- Aspekte der Stammzellforschung Wittenberg (Vorlesung) Dr. U. Scholz (CYG) Genomkartierung/Molekulare Martin-Luther- Naturwissenschaftliche 2 B. Steuernagel (CYG) Diversität Universität Halle- Fakultät III Prof. Dr. A. Graner (GB) Wittenberg Institut für Agrar- und Dr. N. Stein (GB) Ernährungswissenschaften Dr. B. Kilian (GB) K. Wolf (GB) M. Ziems (GB) A. Kusserow (GB) J. Perovic (GB) R.K. Pasam (GB) R. Sharma (GB) Dr. U. Scholz (CYG) Einführung in die Bioinformatik Hochschule Anhalt Fachbereich 7 – 8 Bachelor-Studiengang Köthen Angewandte Biotechnologie Biowissenschaften und (Vorlesung und Übung) Prozesstechnik

* SWS = Semesterwochenstunden

226 Name der/des Lehrenden Thema Universität/ Fakultät/ SWS* Hochschule Fachbereich (FB) Dr. M. Lange (CYG) Datenbankmanagementsysteme Hochschule Harz, Fachbereich 4 Dr. S. Weise (CYG) Bachelor-Studiengang Wernigerode Automatisierung und Medieninformatik Informatik (Vorlesung und Übung) Prof. Dr. T. Altmann (MOG) Projektmodul Molekulare Genetik Martin-Luther- Naturwissenschaftliche 15 Dr. W. Weschke (MOG) Bachelor-Studiengang Biologie Universität Halle- Fakultät I Dr. H. Weber (MOG) (Vorlesung) Wittenberg Institut für Biologie Dr. U. Conrad (MOG) Dr. habil. L. Altschmied (MOG) Dr. S. Friedel (MOG) Dr. habil. L. Altschmied (MOG) Praktikum für Pflanzenphysiologie Friedrich-Schiller- Institut für 4 (Lehramts- und Diplomstudenten) Universität Jena Pflanzenphysiologie Prof. Dr. F. Schreiber (MOG) Analyse biologischer Netzwerke Martin-Luther- Naturwissenschaftliche 4 (Vorlesung und Übung) Universität Halle- Fakultät III Wittenberg Institut für Informatik Prof. Dr. F. Schreiber (MOG) Bioinformatik Christian-Albrechts- Agrar- und Ernährungs 4 Dr. U. Scholz (CYG) (Vorlesung und Übung) Universität zu Kiel wissenschaftliche Fakultät Dr. B. Junker (PZB) Dr. H. Knüpffer (GB) M. Oppermann (GB) Dr. C. Klukas (MOG) Dr. S. Friedel (MOG) Prof. Dr. N. von Wirén (PZB) Transportprozesse über Martin-Luther- Naturwissenschaftliche 1 pflanzliche Membranen Universität Halle- Fakultät I (Vorlesung und Praktikum) Wittenberg Fach Pflanzenphysiologie Prof. Dr. N. von Wirén (PZB) Molekularer Mineralstoffwechsel Martin-Luther- Naturwissenschaftliche 1 (Vorlesung) Universität Halle- Fakultät I Wittenberg Fach Pflanzenphysiologie Priv.-Doz. Dr. H.-P. Mock (PZB) Pflanzenphysiologie Martin-Luther- Naturwissenschaftliche 4 (Grundpraktikum) Universität Halle- Fakultät I Wittenberg Institut für Biologie Dr. J. Kumlehn (PZB) Cytogenetik und Gentechnik Martin-Luther- Naturwissenschaftliche 1,3 (Master-Studiengang) Universität Halle- Fakultät III, Agrarwissen- Wittenberg schaften, Geowissen- schaften und Informatik Prof. Dr. G. Kunze (PZB) Molekulargenetik Teil I Hochschule Anhalt FB Biotechnologie, Lebens- 3 (Vorlesung) Köthen mitteltechnologie, Verfah- rens- und Umwelttechnik Prof. Dr. G. Kunze (PZB) Molekulargenetik Teil II Hochschule Anhalt FB Biotechnologie, Lebens- 3 (Vorlesung) Köthen mitteltechnologie, Verfah- rens- und Umwelttechnik Prof. Dr. G. Kunze (PZB) Biosensoren für die Hochschule Anhalt FB Biotechnologie, 2 Umweltkontrolle (Vorlesung) Köthen Lebensmitteltechnologie, Verfahrens- und Umwelttechnik Prof. Dr. G. Kunze (PZB) Hefegenetik Ernst-Moritz-Arndt- Mathematisch-Naturwissen- 4 (Praktikum) Universität Greifswald schaftliche Fakultät, FB Biologie Dr. B. Junker (PZB) Mikrobiologie Martin-Luther- Pharmazie 2 (Vorlesung) Universität Halle- Wittenberg Dr. B. Junker (PZB) Biogene Arzneimittel Martin-Luther- Pharmazie 1 (Seminar) Universität Halle- Wittenberg Semesterwochenstunden (SWS) insgesamt: 92,8

* SWS = Semesterwochenstunden

227 Mitarbeit an wissenschaftlichen Zeitschriften/Editing Scientific Journals

Mitarbeit an wissenschaftlichen Zeitschriften/ Editing Scientific Journals

Mitarbeiter des Leibniz-Instituts für Pflanzengenetik und Kul- Genetics, Journal of the Genetics Society of America (A. turpflanzenforschung sind Herausgeber bzw. Mitherausgeber Houben, Editorial Board). folgender Zeitschriften: Genetics and Breeding, Bulgarian Academy of Sciences for Agriculture, Piestany, Slovak Republic (A. Börner, Editorial Board). the Bulgarian Genetical Society, Sofia,B ulgaria (I. Schubert, Edi- BBA Gene Regulatory Mechanisms, Elsevier, Maryland torial Board). Heights, USA (A. Houben, Board Member). GM Crops, Landes Bioscience, Austin, USA (J. Kumlehn, Edito- Biotechnology in Forestry and Agriculture, Springer, Berlin- rial Board). Heidelberg (J. Kumlehn, Editor). International Scholarly Research Network (ISRN) Agronomy, Biotechnology Research International, Hindawi, Publishing New York, USA (N. Sreenivasulu, Editorial Board). Corporation, New York, USA (U. Conrad, Subject Editor). Japanese Journal of Breeding Science, The Japanese Society BMC Biotechnology, BioMed Central Ltd, London, UK (U. Con- of Breeding, Tsukuba, Japan (N. Stein, Associate Editor). rad, Associate Editor). Journal of Integrative Bioinformatics (JIB), IMBio, Bielefeld BMC Plant Biology, BioMed Central Ltd, London, UK (N. Stein (F. Schreiber, Associate Editor; M. Lange and U. Scholz, Editorial and N. Sreenivasulu, Associate Editor). Board; C. Klukas, M. Lange and S. Weise, Editorial Management). BMC Research Notes, BioMed Central, London, UK (B. Junker, Journal of Plant Physiology, Elsevier, Amsterdam, The Nether- Associate Editor). lands (J. Kumlehn, Editorial Board). BMC Systems Biology, BioMed Central, London, UK (F. Schrei- Journal of Stem Cells, Nova Science Publishers, Inc., New York, ber, Associate Editor). USA (Anna M. Wobus, Editorial Advisory Board Member). Botanical Journal of Iran, Rostaniha, Tehran, Iran (R. Fritsch, Journal of Tissue Engineering and Regenerative Medicine, Editorial Board). John Wiley & Sons, Ltd., UK (Anna M. Wobus, Editorial Board Cell Biology and Toxicology, Springer, Dordrecht, The Nether- Member). lands (Anna M. Wobus, Consulting Editor). Medizinische Informatik, Biometrie und Epidemiologie, Cells Tissues Organs, Karger AG, Basel, Switzerland (Anna M. Köln (F. Schreiber, Advisory Board). Wobus, Associate Editor). Molecular Breeding, Springer, Dordrecht, The Netherlands Cereal Research Communications, Akadémiai Kiadó, Buda- (A. Graner and J. Kumlehn, Editorial Board). pest, Hungary (A. Börner, Editorial Board). Molecular Plant-Microbe Interactions, APS Press, St. Paul, Chromosoma, Springer, New York, USA (I. Schubert, Associate USA (P. Schweizer, Associate Editor). Editor). New Phytologist, Lancaster, UK (N. von Wirén, Scientific Advisor). Chromosome Research, Springer, Dordrecht, The Netherlands Plant Biotechnology Journal, Blackwell Publishing, Bristol, UK (A. Houben, Editorial Advisory Board). (R. Schmidt, Advisory Board). Comparative and Functional Genomics (T. Altmann, Sup- Plant Cell Reports, Springer, Berlin-Heidelberg (R. Schmidt, porting Editorial Board Member). Editorial Board). Current Trends in Biotechnology and Pharmacy, USA Plant Molecular Biology Reporter, Springer, Berlin-Heidel- (N. Sreenivasulu, Editorial Board Member). berg (R. Schmidt und A. Houben, Editorial Board). Cytogenetics & Genome Research (CGR), Karger AG, Basel, Plant Systematics and Evolution, Springer, Berlin-Heidelberg Switzerland (I. Schubert, Editorial Board). (F.R. Blattner, Editorial Board). Electronic Wheat Information Service, Shizuoka, Japan Proteomics, Wiley-VCH, Weinheim (H.-P. Mock, Editorial Board). (A. Houben, Editorial Advisory Board). Stem Cells, AlphaMed Press, Durham, USA (Anna M. Wobus, Field and Vegetable Crops Research, Novi Sad, Serbia Editorial Board Member). (A. Börner, Editorial Board). The International Journal of Developmental Biology, The Frontiers in Crop Science and Horticulture, Lausanne, Swit- University of the Basque Country Press, Bilbao, Spain (Anna M. zerland (A. Börner, Editorial Board). Wobus, Editorial Advisory Board Member). Frontiers in Plant Genetics and Genomics, Frontiers The Nucleus, MD Publications Pvt Ltd., New Delhi, India Lausanne, Switzerland (I. Schubert, Associate Editor; (I. Schubert, Advisory Board). A. Houben, Editorial Advisory Board). The Open Mycology Journal, Bentham Science Publishers Frontiers in Plant Nutrition, Frontiers Media, Genève, Switzer- Ltd., USA (G. Kunze, Editorial Advisory Board). land (N. von Wirén, Editor-in-Chief). The Plant Journal, Blackwell Publishing, Oxford, UK (T. Alt- Functional & Integrative Genomics, Springer, Berlin-Heidel- mann, Editor). berg (N. Stein, Associate Editor). Theoretical and Applied Genetics, Springer, Berlin-Heidel- Genetic Resources and Crop Evolution (GRACE), Springer, berg (A. Graner, Editorial Board). Dordrecht, The Netherlands (K. Pistrick, Managing Editor; F. R. Vegetable Crops Research Bulletin, RIVC, Skierniewice, Blattner, Editorial Board). Poland (J. Keller, Editorial Advisory Board).

228 Tätigkeit in Gremien/Activities in Boards

Geschäftsführender Direktor Dr. H. Knüpffer − Koordinator des Cereals Network sowie Chairman der Barley Prof. Dr. A. Graner Working Group des European Cooperative Programme for − Mitglied der Deutschen Akademie der Naturforscher LEO- Plant Genetic Resources (ECPGR); POLDINA – Nationale Akademie der Wissenschaften Halle/S.; − Mitglied der Network Coordinating Group des Documenta- − Mitglied des Vorstandsrates der Gesellschaft für Pflanzen- tion and Information Network des ECPGR; züchtung e.V. (GPZ) und Leiter der Ag Genomanalyse; − Mitglied der Arbeitsgruppe zum Europäischen Koopera- − Mitglied des Beirates für nachwachsende Rohstoffe, Ministerium tionsprogramm pflanzengenetischer Ressourcen (ECPGR) für Landwirtschaft und Umwelt des Landes Sachsen-Anhalt; des Beratungs- und Koordinierungsausschusses für pflan- − Vorsitzender des ScientificA dvisory Boards des Max-Planck- zengenetische Ressourcen (BeKo) von Bund und Ländern Instituts für Pflanzenzüchtungsforschung, Köln; (unter Leitung des BMELV); − Mitglied im Wissenschaftlichen Beirat des Julius Kühn-Insti- − Mitglied des International Barley Core Collection Committee tuts (JKI), Quedlinburg; (Bioversity International); − Mitglied des Beratungs- und Koordinierungsausschusses (BEKO) − Mitglied der Arbeitsgruppe Biodiversity Information Stan- des Nationalen Fachprogramms zur Erhaltung und nachhalti- dards (TDWG − ehem. Taxonomic Databases Working Group). gen Nutzung pflanzengenetischer Ressourcen landwirtschaftlicher und gartenbaulicher Kulturpflanzen, BMELV, Bonn; Priv.-Doz. Dr. A. Börner − Mitglied des Wissenschaftlichen Beirates Otto Warburg − Koordinator der European Cereals Genetics Co-operative Center for Agricultural Biotechnology, Hebrew University, (EWAC); Jerusalem, Israel; − Vorsitzender der Cereals Section of the European Associa- − Honorary Fellow des James Hutton Institute, Dundee, UK; tion for Research on Plant Breeding (EUCARPIA); − Steering Committee, International Barley Sequencing Con- − Mitglied des Seed Storage Committee of the International sortium (IBSC); Seed Testing Association (ISTA); − Stellvertretender Vorsitzender der Gemeinschaft zur Förde- − German Representative of Working Group Wheat of the Eu- rung der Kulturpflanzenforschung Gatersleben e.V.; ropean Cooperative Programme for Plant Genetic Resources − Review Panel Research Program: Integrated Biological Sys- (ECPGR); tems, Australian Government Department of Innovation, − Vorsitzender der Arbeitsgemeinschaft Saatgut und Sor- Industry, Science and Research (DIISR) (2010); tenwesen der Gesellschaft für Pflanzenbauwissenschaften − Mitglied Executive Board, Generation Challenge Program, Con- (GPW) und der Gesellschaft für Pflanzenzüchtung (GPZ); sultative Group of International Agricultural Research (CGIAR); − Mitglied der Beratungsgruppe Entwicklungsorientierte − Mitglied des Direktoriums, Interdisziplinäres Zentrum für Nutz- Agrarforschung (BEAF) der Deutschen Gesellschaft für Tech- pflanzenforschung (IZN), Martin-Luther-Universität Halle/S; nische Zusammenarbeit (GTZ). − acatech, Arbeitsgruppe Anpassungsstrategien in der Klima- politik; Dr. U. Lohwasser − Mitglied des Kuratoriums der Sparkassenstiftung Aschersle- - Mitglied des Expertengremiums zur Entwicklung von Gen- ben-Staßfurt; bankstandards bei Bioversity; − Vorsitzender des Fördervereins des Schülerlabors „Grünes - Core Advisory Group bzw. Reviewer bei Bioversity; Labor Gatersleben“. - Vorstandsmitglied in der Gemeinschaft zur Förderung der Kulturpflanzenforschung; - Mitglied des beratenden Gremiums zum Aufbau der Gen- banken WEL und Zierpflanzen in Deutschland; Abteilung Genbank - Deutscher Vertreter of Working Group Solanaceae of the Eu- ropean Cooperative Programme for Plant Genetic Resources Dr. N. Stein (ECPGR). − Chair of International Barley Genome Sequencing Consor- tium (IBSC, http://barleygenome.org); Dr. J. Keller − European Triticeae Genomics Initiative (ETGI, http://www. − Mitglied der Koordinierungsgruppe des ECPGR Vegetables etgi.org) (Koordinator); Network und Vice Chairman der Allium-Arbeitsgruppe; − Management Committee and National Representative of − Chairman der Allium Working Group im European Coopera- COST-Action Tritigen FA0604; tive Programme for Plant Genetic Resources (ECPGR); − Chair of the Scientific Coordinating Committee (SCC) of the − Mitglied im Lenkungsausschuss der europäischen COST- German Plant Biotechnology Program. Initiative 871 „Kryokonservierung in Europa“.

229 Tätigkeit in Gremien/Activities in Boards

Dr. K.J. Dehmer − Mitglied des Scientific Advisory Board of the Nottingham − Mitglied in der ECPGR Working Group on Potato; Arabidopsis Stock Centre (NACS); − Councellor der European Association for Potato Research − Program Board Member of the DFG-funded Program (EAPR). „Heterosis bei Pflanzen”. − Mitglied im Wissenschaftlichen Beirat der Gemeinschaft zur E. Willner Förderung der privaten deutschen Pflanzenzüchtung e.V. − Stellvertretende Vorsitzende der ECPGR Working Group on (GFP). Forages. Prof. Dr. F. Schreiber Dr. K. Pistrick − Mitglied des Lenkungsgremiums der Gesellschaft für Infor- − Mitglied im Nomenclature Committee of the International matik (GI) und der Gesellschaft für Medizinische Informatik, Seed Testing Association (ISTA). Biometrie und Epidemiologie (GMDS) des Fachbereichs „In- formatik in den Lebenswissenschaften“; − Mitglied im German/Russian Virtual Network on Bioinfor- matics; Abteilung Cytogenetik und Genomanalyse − Mitglied im Sino/German Network of Computational & Inte- grative Biology; Priv.-Doz. Dr. R. Schmidt − Sprecher der Fachgruppe Informationsmanagement in der − Gewähltes Mitglied des DFG-Fachkollegiums „Pflanzenwis- Biotechnologie der Gesellschaft für Informatik (GI). senschaften“.

Prof. Dr. Anna M. Wobus − Mitglied der Deutschen Akademie der Naturforscher Abteilung Physiologie und Zellbiologie LEOPOLDINA – Nationale Akademie der Wissenschaften, Halle/S.; Prof. Dr. N. von Wirén − Ordentliches Mitglied der Berlin-Brandenburgischen Aka- − Mitglied des „IPNC-Steering Committees“ (International demie der Wissenschaften (BBAW); Plant Nutrition); − Mitglied der Zentralen Ethik-Kommission für Stammzellen- − Vizepräsident der Deutschen Gesellschaft für Pflanzener- forschung (ZES) am Robert-Koch-Institut, Berlin (stellvertre- nährung; tende Vorsitzende); − Fachgutachter der Deutschen Forschungsgemeinschaft im − Mitglied des Novartis Ethics Advisory Board (NEAB) von Fachkollegium 207 „Agrar-, Forstwissenschaften, Gartenbau NOVARTIS Pharma International, Basel, Schweiz; und Tiermedizin“; − Mitglied des Programmbeirats des Wissenschaftszentrums − Mitglied des Scientific Advisory Boards des „Department of Sachsen-Anhalt (WZW). Agriculture & Ecology of the University of Copenhagen“; − Mitglied des Wissenschaftlichen Beirats des Leibniz-Instituts Dr. habil. P. Schweizer für Pflanzenbiochemie, Halle/S.; − Mitglied der Zentralen Kommission für Biologische Sicher- − Mitglied des Direktoriums des Leibniz-Wissenschaftscam- heit (ZKBS), Bereich Pflanzenzucht. pus „Pflanzenbasierte Bioökonomie“ mit der Martin-Luther- Universität Halle-Wittenberg, Halle/S.

Prof. Dr. G. Kunze Abteilung Molekulare Genetik − Mitglied im wissenschaftlichen Beirat der Fa. ARTES Biotech- nology GmbH. Prof. Dr. T. Altmann − Mitglied des Multinational Arabidopsis Steering Commit- Dr. B.H. Junker tee; − Mitglied im Zukunftsforum Biotechnologie der DECHEMA − Mitglied des Scientific Co-Ordination Committee (SCC) of e.V., Arbeitskreis Systembiologie und Synthetische Biologie; the BMBF-funded German Plant Biotechnology Program; − Mitglied der Fachgruppe Systembiologie und Synthetische − Mitglied des Scientific Advisory Board of the French plant Biologie der DECHEMA e.V. genome program Génoplante;

230 Öffentlichkeitsarbeit/Public Relations Informationsveranstaltungen und Führungen/Informative Events and Guided Tours

2010 31. März 2010 Führung durch die Malchower Öl- und Futterpflanzen-Sorti- 8. Januar 2010 mente der IPK-Genbank für Futterpflanzenzüchter derN PZ und Besuch von Studenten des 1. Semesters Landwirtschaft (Bache- der Universität Kiel, 5 Personen, Sammelreisestrategie für alte lorstudiengang) der Hochschule Anhalt, Köthen, 30 Personen, Grünlandstandorte (E. Willner). Vorstellung des Instituts, Besuch der Abteilungen Molekulare Genetik und Cytogenetik und Genomanalyse, Besichtigung der 7. April 2010 Genbank, des Samenkühllagers und der In vitro-Erhaltung und Besuch von Lehrern der Sächsischen Bildungsagentur (SBA) Kryolagerung (R. Schnee, Dr. habil. H. Bäumlein, Dr. A. Houben, Chemnitz, 20 Personen, Überblick zur Geschichte der Biotech- Dr. U. Lohwasser, Dr. J. Keller). nologie und zur Genregulation bei Pflanzen, Produktion von Vakzinen und Antikörpern in Pflanzen, Anwendung von Hefen 13. Januar 2010 in der Pflanzenforschung und zur Entwicklung von Hormon- Besuch von Studenten der Agrarwirtschaft an der Fachhoch- Sensoren, Führung durch die Labore der Arbeitsgruppe Hefe- schule Soest, 11 Personen, Vorstellung des Instituts und der genetik, Neue Verfahren und Methoden in der Chromosomen- Aufgaben der Genbank, Führung durch die Genbank, Elektro- biologie, Systembiologie – an der Schnittstelle zwischen Dry nenmikroskopie in der Pflanzenforschung und Besichtigung und Wetlab, Erzeugung von transgenen Pflanzen,B esichtigung der Gewächshäuser (R. Schnee, Dr. U. Lohwasser, Dr. M. Melzer, der Gewächshäuser und Gewebekulturen, Rundgang im Grü- E. Geyer). nen Labor (Dr. habil. H. Bäumlein, Priv.-Doz. Dr. U. Conrad, Prof. Dr. G. Kunze, Dr. A. Houben, Dr. B. Junker, Dr. J. Kumlehn). 22. Januar 2010 Besuch von Studenten des 1. Semesters Landwirtschaft (Bache- 12. April 2010 lorstudiengang) der Hochschule Anhalt, Köthen, 30 Personen, Besucher vom Umwelt-, Planungs-, Verkehrs- und Wirtschafts- Vorstellung des Instituts, Besuch der Abteilungen Molekulare ausschuss im Biotech-Gründerzentrum Gatersleben, 13 Perso- Genetik und Cytogenetik und Genomanalyse, Besichtigung nen, Erläuterung der Aufgaben in der Genbank mit anschlie- der Genbank mit Samenkühllager, Labor und In vitro-Erhaltung ßender Führung (Priv.-Doz. Dr. A. Börner). und Kryo-Lagerung (R. Schnee, Dr. habil. H. Bäumlein, Dr. A. Houben, Dr. U. Lohwasser, Dr. J. Keller). 28. April 2010 Führung durch die Malchower Öl- und Futterpflanzen-Sorti- 27. Januar 2010 mente der IPK-Genbank im Rahmen des 10. ECPGR working Besuch von Herrn Detlef Gürth, MdL Sachsen-Anhalt, wirt- group meeting on forages, 30 Personen, Vorstellung der Ar- schaftspolitischer Sprecher der CDU-Fraktion, Besuch der Gen- beitsweise nach QMS, insbesondere Dokumentation: GBIS mit bank und Rundgang durch das IPK und SunGene, Vorstellung C- und E-Daten-Verwaltung (S. Hünmörder, V. Miehe, M. Opper- der Forschungsschwerpunkte Grüne Gentechnik und Diskus- mann, E. Willner). sion über den Standort (Prof. Dr. A. Graner, Prof. Dr. Th. Alt- mann, Prof. Dr. I. Schubert, Prof. Dr. N. von Wirén, S.-A. Lorenz, 30. April 2010 R. Schnee). Besuch von Berufsberatern der Arbeitsagentur Harzkreis, 12 Personen, Überblick über Ausbildungsmöglichkeiten am 27. Januar 2010 IPK (Bürokauffrau/-mann, Biologielaborant/-in, Gärtner/-in, Genbankführung für Dr. Likyelesh Gugsa aus Äthiopien (Dr. U. Bibliothekassistent/-in), Besichtigung eines Labors und Ge- Lohwasser, Dr. J. Keller). wächshauses sowie der Bibliothek, Rundgang im Grünen Labor (C. Höpfner, J. Becker, Priv.-Doz. Dr. A. Börner, J. Marlow, S. Win- 18. Februar 2010 ter, S. Amme). Besuch von Mitarbeitern der Psychiatrischen Tagesklinik, Aschersleben, 20 Personen, Vorstellung des Instituts sowie der 30. April 2010 Aufgaben der Genbank und anschließende Besichtigung, Er- Führung durch die Malchower Öl- und Futterpflanzen-Sorti- läuterung der In vitro-Erhaltung und Kryo-Konservierung, Ge- mente der IPK-Genbank im Rahmen des 10. ECPGR working wächshausführung und Besichtigung des Phytokammerhauses group meeting on forages, 3 Personen, vertiefende Vorstellung (Priv.-Doz. Dr. A. Börner, Dr. J. Keller, P. Schreiber, E. Geyer). der Arbeitsweise nach QM-Standards, insbesondere Vermeh- rungsstandards, Isolationen (E. Willner).

231 Öffentlichkeitsarbeit/Public Relations

3. Mai 2010 3. Juni 2010 Besuch von Fachschülern der Landesanstalt für Landwirtschaft, Teilnehmer am EXBARDIV-Meeting-Projekt, 14 Personen, Vor- Forsten und Gartenbau, Fachschule für Landwirtschaft, Garten- stellung der Aufgaben der Genbank, Genbankführung und bau und Hauswirtschaft, Quedlinburg, 9 Personen, Vorstellung Feldbesichtigung (Dr. U. Lohwasser, P. Schreiber). des Instituts, In vitro-Erhaltung und Kryokonservierung von Kul- turpflanzen,B esichtigung des Herbars sowie der Samensamm- 5. Juni 2010 lung, Gewächshausbesichtigung (Dr. J. Keller, Dr. K. Pistrick, J. Führungen zum „Tag der offenenT ür“ der Malchower Sortimen- Marlow). te für Öl- und Futterpflanzen der IPK-Genbank, ca. 250 Perso- nen, Rundgänge und Erläuterungen zum Thema „2010 – Jahr 5. Mai 2010 der Biologischen Vielfalt“ im Schaugarten bzw. im Feldanbau Besuch von Mitgliedern des Rotary Club aus Sandy, UK, 3 Per- und in den Arbeitsräumen (M. Hautmann, P. Hermann, S. Hün- sonen, Vorstellung des Instituts, Besichtigung des Genomzen- mörder, R. Rudloff, H. Schmalfeldt, E. Willner unter Mithilfe von trums und der Genbank (Prof. Dr. A. Graner, Dr. habil. P. Schwei- B. Fietz, H. Weiß). zer, Priv.-Doz. Dr. A. Börner). 8. Juni 2010 7. Mai 2010 Besuch des Freundeskreises der Fakultät für Physik und Geo- Besuch des Staatssekretärs Herrn Jürgen Stadelmann und wissenschaften, Leipzig, 40 Personen, Vorstellung des Instituts, Herrn Karl-Heinz Weege vom Ministerium für Landwirtschaft Besichtigung der Genbank mit Kühllager, Keimfähigkeitstest- und Umwelt des Landes Sachsen-Anhalt, Vorstellung des In- labor und Dreschscheune (R. Schnee, Priv.-Doz. Dr. A. Börner, stituts mit anschließender Diskussion, Führung Lemnatec-An- Dr. A. Meister). lage, Besuch Grünes Labor (Prof. Dr. A. Graner, Prof. Dr. Th. Alt- mann, Prof. Dr. I. Schubert, S.-A. Lorenz, Priv.-Doz. Dr. H.-P. Mock, 9. Juni 2010 Dr. habil. P. Schweizer, Prof. Dr. F. Schreiber, I. Mücke). Besuch von Mitgliedern der GFP-Arbeitsgruppe Getreide, 50 Personen, Führung durch die Botanischen Vergleichssammlun- 10. Mai 2010 gen (Dr. K. Pistrick). Führung von Ph. D. Efisio Mattana, Centro Conservazione Bio- diversità, Università degli Studi di Cagliari, Cagliari, Italien, Vor- 11. Juni 2010 stellung des Instituts und der Aufgaben der Genbank, Führung Besuch von Studenten der Universität Rostock, Agrar- und Um- durch die Genbank, Erläuterung der In vitro-Erhaltung und weltwissenschaftliche Fakultät/ Agrobiotechnologie, 10 Perso- Kryokonservierung (Priv.-Doz. Dr. A. Börner, Dr. J. Keller). nen, Führung durch die Groß Lüsewitzer Kartoffel-Sortimente der IPK-Genbank (M. Angeli, U. Behrendt, Dr. K.J. Dehmer). 11. Mai 2010 Besucher im Rahmen der Landesgartenschau (LAGA), Aschers- 12. Juni 2010 leben, 4 Personen, Darstellung der Aufgaben einer Genbank, Besucher zum „Tag der offenen Tür“, ca. 60 Personen, Besichti- Führung durch die Botanischen Vergleichssammlungen zum gung der Genbank, Führung durch die Botanischen Vergleichs- Thema Allium-Taxonomie (Priv.-Doz. Dr. A. Börner, Dr. J. Keller, sammlungen des Bereiches Taxonomie und Evolution (Herba- Dr. K. Pistrick, M.-L. Graichen). rium, Samen- und Fruchtsammlung sowie Ährensammlung, Präsentation der Teilsammlungen Nord der IPK-Genbank (Priv.- 17. Mai 2010 Doz. Dr. A. Börner, Dr. K. Pistrick, Dr. U. Lohwasser, M. Kotter, Besuch eines Freundeskreises aus Friedrichsaue, 8 Personen, Dr. K. J. Dehmer, Dr. J. Keller, D. Büchner, G. Matzig, B. Schmidt, Führung durch den Staudengarten (B. Schütze). R. Kurch, M.-L. Graichen).

17. Mai 2010 14. Juni 2010 Mitarbeiter der Firma Norika, 2 Personen, Führung durch die Besuch von Studenten der Universität Hohenheim, Institut Groß Lüsewitzer Kartoffel-Sortimente der IPK-Genbank (M. An- für Pflanzenzüchtung, Saatgutforschung und Populationsge- geli). netik (350), Stuttgart, 15 Personen, Vorstellung der Genbank, Genbankdokumentation, In vitro-Erhaltung und Kryokonservie- 26. Mai 2010 rung von Kulturpflanzen, Führung durch die Herbarsammlun- Besucher des Landseniorenvereins Salzwedel, 42 Personen, gen und durch die Vermehrungsbestände der Genbank (Priv.- Vorstellung des Instituts und der Aufgaben der Genbank, Gen- Doz. Dr. A. Börner, Dr. H. Knüpffer, Dr. J. Keller, Dr. K. Pistrick, bankführung und durch die Herbar- und Samensammlung, P. Schreiber). Führung durch die Vermehrungsflächen und Gewächshäuser der Genbank, Diskussion über Gentechnik (Dr. U. Lohwasser, Dr. K. Pistrick, Dr. J. Kumlehn).

232 15. Juni 2010 1. bis 2. Juli 2010 Besucher der Landesgartenschau (LAGA) Aschersleben, 4 Workshop „Bestimmung von Schlafmohn und anderen Mohn- Personen, Darstellung der Aufgaben einer Genbank, Füh- arten“, Kriminalämter der BRD, 10 Personen, Vorstellung der rung durch den Anbau des Salatsortiments (Dr. U. Lohwasser, Aufgaben der Genbank, Forschung an Schlafmohn, Einführung S. Thumm, M.-L. Graichen). der Mohnarten sowie Feldbesichtigung Mohnanbau, Führung durch die Genbank (Priv.-Doz. Dr. A. Börner, Dr. U. Lohwasser, 16. Juni 2010 R. Kurch). Besuch von Studenten und Mitarbeitern des Instituts für Pflan- zenbau und Pflanzenzüchtung der Universität Göttingen, 4. Juli 2010 27 Personen, Vorstellung des Instituts und der Aufgaben der Besuch einer Gruppe ehemaliger Agraringenieure aus Berlin, Genbank, Genbankführung, Informationen über die Aufgaben 15 Personen, Führung durch die Malchower Sortimente für der Genbankdokumentation sowie Führungen in der In vitro- Öl- und Futterpflanzen der IPK-Genbank und Erläuterung der Erhaltung und Kryokonservierung, Herbar- und Samensamm- Arbeit einer Genbank (E. Willner). lung, automatisierte Phänotypisierung und Feldführung (Dr. U. Lohwasser, Dr. J. Keller, Dr. H. Knüpffer, Dr. K. Pistrick). 7. Juli 2010 Besuch eines Kartoffelzüchters mit Mitarbeiterin, 2 Personen, 16. Juni 2010 Führung durch die Groß Lüsewitzer Kartoffel-Sortimente der Besuch von Vertretern des Landwirtschaftsministeriums Meck- IPK-Genbank (U. Behrendt). lenburg-Vorpommern, 3 Personen, Führung durch die Groß Lüsewitzer Kartoffel-Sortimente der IPK-Genbank (Dr. K.J. Deh- 7. Juli 2010 mer, E. Willner). Besuch von Studenten der Martin-Luther-Universität Halle- Wittenberg, 5 Personen, Vorstellung des Instituts, Grüne Gen- 16. Juni 2010 technik, Besichtigung der Arbeitsgruppe Pflanzliche Reproduk- Besuch von Mitarbeitern des Pflanzenschutzdienstes Rostock tionsbiologie (R. Schnee, Dr. J. Kumlehn). mit Gästen des Plant Protection Directorats Serbien, 5 Perso- nen, Führung durch die Groß Lüsewitzer Kartoffel-Sortimente 9. Juli 2010 der IPK-Genbank (Dr. K.J. Dehmer, U. Behrendt). Besuch von Interessenten der BioTech Farm GmbH & Co. KG, Üplingen, 6 Personen, Vorstellung der Genbank, Freilandversu- 18. Juni 2010 che, Rundgang durchs Institut (R. Schnee). Besuch von Mitarbeitern und Kunden einer Kartoffel-Vermark- tungsgesellschaft, 45 Personen, Vorstellung der Groß Lüsewit- 10. Juli 2010 zer Kartoffel-Sortimente der IPK-Genbank (Dr. K.J. Dehmer). Besuch von Vorstandsmitgliedern und Aufsichtsrat der Milchtrocknung Süd-Hannover (MTS), Sitz in Harbarnsen bei 24. Juni 2010 Hildesheim, 15 Personen, Vorstellung des Instituts und der Besuch des Genbank-Beirats, 7 Personen, Führung durch die Aufgaben der Genbank, Genbankführung sowie Feld- und Ge- Malchower Öl- und Futterpflanzen-Sortimente der IPK-Gen- wächshausführung (Priv.-Doz. Dr. A. Börner). bank, insbesondere QM-Standards, Genbankdokumentations- system GBIS, Vermehrungs- und Versuchsanbau (E. Willner). 14. Juli 2010 Vertreter des Landwirtschaftsministeriums Mecklenburg-Vor- 25. Juni 2010 pommern, 2 Personen, Führung durch die Malchower Öl- und Besuch des Genbank-Beirats, 7 Personen, Führung durch die Futterpflanzen-Sortimente der IPK-Genbank (Dr. K.J. Dehmer, Groß Lüsewitzer Kartoffel-Sortimente der IPK-Genbank, ins- E. Willner). besondere in vitro-Kultur, Wildkartoffelvermehrung und Fel- danbau (Dr. K.J. Dehmer, M. Angeli, U. Behrendt, K. Diekmann, 20. Juli 2010 K. Göhrke, M. Vandrey). Besucher der Landesgartenschau (LAGA), Aschersleben, 6 Per- sonen, Darstellung der Aufgaben einer Genbank, Führung 30. Juni 2010 durch den Anbau des Hülsenfrüchtesortiments (Dr. U. Lohwas- Besuch vom Centre for Genetic Resources (CGN), Niederlande, ser, M. Kotter). 3 Personen, Vorstellung der Aufgaben der Genbank, Führun- gen der In vitro-Erhaltung und Kryokonservierung, Führung im 22. Juli 2010 Gewächshaus und Feldbesichtigung sowie Informationen über Besuch von Mitgliedern des Wirtschaftsclubs Quedlinburg, 18 die Herbarsammlung und das Aufgabengebiet der Dokumen- Personen, Vorstellung des Instituts, Genbankführung, Führung tation (Priv.-Doz. Dr. A. Börner, Dr. J. Keller, Dr. A. Senula, Dr. U. durch das Genomzentrum (Prof. Dr. A. Graner, Dr. U. Lohwasser, Lohwasser, B. Schmidt, M.-L. Graichen, S. Thumm, M. Opper- Dr. habil. L. Altschmied). mann).

233 Öffentlichkeitsarbeit/Public Relations

30. Juli 2010 23. August 2010 Besuch des WGL-Präsidenten, Prof. Karl Ulrich Mayer, und sei- Auszubildende des Julius Kühn-Instituts Groß Lüsewitz, 7 Per- ner Referentin, Dr. Nicola Isendahl, Berlin, Vorstellung des Insti- sonen, Führung durch die Groß Lüsewitzer Kartoffel-Sortimen- tuts, Vortrag „Grüne Gentechnik am IPK“, Rundgang Genbank, te der IPK-Genbank (U. Behrendt, K. Göhrke, M. Vandrey). Genomzentrum und Staudengarten, Abschlussdiskussion (Prof. Dr. A. Graner, Prof. Dr. I. Schubert, Prof. Dr. Th. Altmann, S.- 28. August 2010 A. Lorenz, Prof. Dr. F. Schreiber, Dr. F. Blattner, Dr. U. Lohwasser, Exkursion der EGF-Tagung (European Grass Foundation), Kiel, Dr. habil. P. Schweizer, Dr. J. Kumlehn, R. Schnee, N. Schmid, M. 44 Personen, Führung durch die Malchower Sortimente für Mau, L. Gerson, K. Menzel). Öl- und Futterpflanzen der IPK-Genbank und Erläuterung der Arbeit einer Genbank (K. Ploen, E. Willner). 3. August 2010 Besuch von Lehrern aus Staßfurt, 60 Personen, Vorstellung des 3. September 2010 Instituts und der Aufgaben der Genbank, Führung durch die Besuch von Mitarbeitern der Ag Heterosis des IPK, 12 Personen, Genbank sowie Feld- und Staudengartenführung (Dr. U. Loh- Führung durch die Sortimente für Öl- und Futterpflanzen der wasser, P. Schreiber, J. Marlow). IPK-Genbank und Erläuterung der Arbeit der Teilsammlungen Nord mit Schwerpunkt Ölpflanzen (K. Ploen, E. Willner). 4. August 2010 Besuch des Lehrerkollegiums der Sekundarschule „Maxim Gor- 6. September 2010 ki“, Schönebeck, 32 Personen, Vorstellung des Instituts, Besich- Besuch von russischen Wirtschaftsvertretern, Organisation tigung des Genomzentrums und der Lemnatec-Gewächshaus- über die Deutsche Management Akademie Niedersachsen, anlage (Dr. habil. L. Altschmied, I. Mücke, R. Schnee). Celle, 18 Personen, Vorstellung des Instituts, Campusrundgang, Genbankbesichtigung, Besichtigung der Gewächshäuser Ge- 5. August 2010 netik und am Genomzentrum (R. Schnee). Besuch von Herrn Veit Wolpert, Landesvorsitzender der FDP- Fraktion Sachsen-Anhalt, in Begleitung eines Referenten, einem 7. September 2010 InnoPlanta-Mitarbeiter und zwei dpa-Journalisten, Vorstellung Besucher der Landesgartenschau (LAGA) Aschersleben, 25 Per- der Aufgaben der Genbank und anschließende Genbankfüh- sonen, Darstellung der Aufgaben einer Genbank, Führung im rung (Dr. U. Lohwasser, R. Schnee). Dauergarten des Kräutersortiments (Priv.-Doz. Dr. A. Börner, R. Kurch). 5. August 2010 Gast aus Japan in der Ag Genomdiversität, Vorstellung der Auf- 8. September 2010 gaben der Genbank und Genbankführung (Dr. U. Lohwasser). Besucher aus dem Institut für Spezielle Zoologie und Evoluti- onsbiologie, Jena, 8 Personen, Vorstellung des Instituts und der 17. August 2010 Genbank mit anschließender Führung, Vorstellung der taxono- Besucher der Landesgartenschau (LAGA) Aschersleben, 17 mischen Sammlungen in Vorbereitung der Sonderausstellung Personen, Darstellung der Aufgaben einer Genbank, Führung „Domestikation“ des Phyletischen Museums (Priv.-Doz. Dr. A. durch den Anbau des Tomatensortiments (Dr. U. Lohwasser, Börner, Dr. K. Pistrick, M. Grau). B. Schmidt). 11. September 2010 18. August 2010 Besuch von Mitgliedern der Freiwilligen Feuerwehr Grimma, Besuch von Frau Prof. Dr. Ulla Bonas, MLU Halle-Wittenberg, 40 Personen, Vorstellung des Instituts und der Genbank mit Vorstellung der Aufgaben der Genbank und Genbankführung anschließender Führung sowie eine Feld- und Gewächshaus- (Priv.-Doz. Dr. A. Börner). führung (Priv.-Doz. Dr. A. Börner).

23. bis 27. August 2010 15. September 2010 Besuch von Frau L. Stimolo, Genbank Bari, Italien, Einführung in Besuch von Mitgliedern des Bundesverbandes für Teilnehmer- die Aufgaben der Genbank, Vorstellung des Genbankinforma- gemeinschaften e.V. (BTG), Schönebeck, 67 Personen, Vorstel- tionssystems (GBIS) und Genbankdokumentation, Erläuterung lung des Instituts, Bewahrung von Pflanzenschätzen,E rfassung des Qualitätsmanagementsystems der Genbank, Besichtigung pflanzlicher Merkmale „am laufenden Band“ (R. Schnee, Dr. C. der Herbar- und Samensammlung, Erläuterung der in vitro- Klukas, I. Mücke). Erhaltung und Kryokonservierung, Führung im Gewächshaus und auf dem Feld, Durchführung der Charakterisierung der 21. September 2010 Kürbiskollektion sowie ein Abschlussgespräch (Priv.-Doz. Dr. A. Besucher der Landesgartenschau (LAGA), Aschersleben, 25 Börner, Dr. U. Lohwasser, Dr. K. Pistrick, Dr. C. Zanke, M. Opper- Personen, Darstellung der Aufgaben einer Genbank, Führung mann, B. Schmidt). durch den Anbau des Kürbissortiments (Dr. U. Lohwasser, B. Schmidt).

234 22. September 2010 20. Oktober 2010 Besuch von Mitgliedern des Vereins der Freunde des Märki- Besuch von Prof. F. Corbineau, Pierre und Marie Curie-Universi- schen Ausstellungs- und Freizeitzentrums (MAFZ), 26 Personen, tät Paris, Besichtigung der Botanischen Vergleichssammlungen Vorstellung der Aufgaben der Genbank und anschließende (Dr. K. Pistrick). Genbankführung sowie eine Feld- und Gewächshausführung (Priv.-Doz. Dr. A. Börner). 21. Oktober 2010 Teilnehmer am Begleitprogramm anlässlich der Jahreskonfe- 27. September 2010 renz der Ministerpräsidentinnen und Ministerpräsidenten der Besuch von Auszubildenden als landwirtschaftlich-technische deutschen Bundesländer in Magdeburg, 7 Personen, Vorstel- Angestellte der Fachschule Lüneburg, 22 Personen, Vorstellung lung des Instituts, Rundgang auf dem Gelände des IPK und Be- des Instituts, Genbankführung, Demonstration der Variabili- sichtigung der LemnaTec-Anlage im Gewächshaus der Genetik, tät von Kulturpflanzen im Herbarium sowie in der Ähren- und Besichtigung der Kühllager der Bundeszentralen Ex-situ-Gen- Früchtesammlung, Variabilität in den Gewächshäusern der bank für landwirtschaftliche und gartenbauliche Kulturpflan- Genbank (R. Schnee, Dr. U. Lohwasser, Dr. K. Pistrick, J. Marlow). zen, Samenaufbereitung in der Genbank (R. Schnee, Priv.-Doz. Dr. A. Börner). 28./29. September 2010 Besuch einer Schülergruppe der Freien Schule Neinstedt, 12 22. Oktober 2010 Personen, Einführende Erläuterungen zu den Grundlagen Besuch von australischen Kartoffelzüchtern, 2 Personen, Füh- der Dokumentation von Pflanzenmaterial in Botanischen Ver- rung durch die Groß Lüsewitzer Kartoffel-Sortimente der IPK- gleichssammlungen (Dr. K. Pistrick). Genbank (Dr. K.J. Dehmer).

2. Oktober 2010 26. Oktober 2010 Besuch von Mitgliedern des Lions Club, Bad Driburg, 42 Perso- Besuch von kubanischen Kartoffelzüchtern als Gäste der Ag- nen, Vorstellung des Instituts und der Aufgaben der Genbank, rar- und Umweltwissenschaftlichen Fakultät der Universität Genbankführung, Variabilität in den Gewächshäusern der Gen- Rostock, 3 Personen, Führung durch die Groß Lüsewitzer Kar- bank und im Tropenhaus, Molecular Ph(F)arming – eine Chan- toffel-Sortimente der IPK-Genbank (Dr. K.J. Dehmer). ce für die Landwirtschaft der Zukunft? (Priv.-Doz. Dr. A. Börner, Priv.-Doz. Dr. U. Conrad, J. Marlow). 2. bis 4. November 2010 Besuch von Frau M. Stein, Head of the Evogene Seed Bank, Is- 7. Oktober 2010 rael, Vorstellung der Aufgaben der Genbank und anschließen- Besuch von Wissenschaftlern der UC Davis, USA, 2 Personen, de Genbankführung, Gespräche mit den Sortimentsgruppen, Vorstellung des Instituts und der Aufgaben der Genbank, an- Informationen über das GBIS-System, in vitro-Erhaltung, Kryo- schließende Genbankführung (Prof. Dr. A. Graner, Priv.-Doz. Dr. lagerung, Herbarium, Gewächshaus und Qualitätsmanage- A. Börner). ment (Priv.-Doz. Dr. A. Börner, K. Krusch, K. Bollmann, M. Op- permann, D. Dittmann, Dr. J. Keller, Dr. K. Pistrick, J. Marlow, 7. Oktober 2010 Dr. U. Lohwasser). Besuch von Schülern der 3. und 4. Klassen der Grundschule Sanitz, ca. 80 Personen, Führung durch die Groß Lüsewitzer 8. November 2010 Kartoffel-Sortimente derI PK-Genbank (U. Behrendt, K. Göhrke). Besuch von Studenten des Studiengangs Agrarökologie der Universität Rostock, 6 Personen, Führung durch die Groß Lüse- 12. Oktober 2010 witzer Kartoffel-Sortimente derI PK-Genbank (Dr. K. J. Dehmer). Führung durch die Groß Lüsewitzer Kartoffel-Sortimente der IPK-Genbank für Kartoffel-Interessenten, 2 Personen (U. Beh- 10. November 2010 rendt, K. Göhrke, S. Pelikan). Besuch einer Delegation von Wissenschaftlern aus dem Kö- nigreich Jordanien, 5 Personen, Vorstellung des Instituts, Vor- 13. Oktober 2010 stellung der Forschungsinteressen der jordanischen Delegati- Besuch von Teilnehmern des 1. Vergabekongresses Sachsen- on, Diskussion möglicher Kooperationsthemen zur Saat- und Anhalt, 40 Personen, Vorstellung der Aufgaben der Genbank Pflanzenzucht, Institutsbesichtigung (Prof. Dr. I. Schubert, Prof. und Genbankführung, Warum Frost für Pflanzensamen gut ist, Dr. G. Kunze, Dr. N. Sreenivasulu, Dr. T. Schnurbusch, Ahmad M. Bestimmung von Pflanzen: der Nutzen von Herbar-Sammlun- Alqudah, Dr. B. Kilian, R. Schnee). gen, Reise ins Innere der Pflanzen (Priv.-Doz. Dr.A . Börner, Dr. K. Pistrick, Dr. M. Melzer). 11. November 2010 Besuch vom Bundessortenamt Hannover, 3 Personen, Vorstel- lung der Aufgaben der Genbank und Genbankführung (Priv.- Doz. Dr. A. Börner, Dr. U. Lohwasser, M. Oppermann, M. Kotter).

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11. November 2010 15. Dezember 2010 Besuch von Schülern der 9. Klasse der ecolea, Internationale Besuch von Studenten der Fachhochschule Südwestfalen, Schule Warnemünde, 25 Personen, Führung durch die Groß Fachbereich Agrarwirtschaft, Soest, 15 Personen, Vorstellung Lüsewitzer Kartoffel-Sortimente der IPK-Genbank (Dr. K.J. Deh- des Instituts, Anwendung von Assoziationskartierung zur Ver- mer, U. Behrendt, K. Göhrke). besserung der Malzqualität bei Gerstensorten, Was ist Epigene- tik?, anschließende Besichtigung der Ag Epigenetik mit einem 12. und 18. November 2010 „Blitzversuch“ der GUS-Reportergenexpression, Führung durch Besuch von Schülern anlässlich der Berufsorientierungstage des die Kühlräume der Genbank, Vorstellung der In vitro-Erhaltung Grünen Labors Gatersleben, 40 Personen, Führung und leichte der Genbank (R. Schnee, Dr. M. Röder, Dr. M.F. Mette, Priv.-Doz. Arbeiten im Gewächshaus der Genbank, Analyse gesundheits- Dr. A. Börner, Dr. Ch. Zanke). förderlicher Pflanzeninhaltsstoffe (J. Marlow, A. Dittbrenner). 16. Dezember 2010 18. November 2010 Besuch der Universität Florenz, Italien, Bereich Landwirtschaft Besuch einer Seminargruppe für ein Freiwilliges Ökologisches und Pflanzengenetik, 6 Personen, Vorstellung des Instituts und Jahr (FÖJ), Jugendwerk Aufbau Ost e.V., Berlin, 30 Personen, der Genbank mit anschließender Besichtigung, Vorstellung Vorstellung des Instituts, Biodiversität in der Genbank, Gen- der In vitro-Erhaltung und Besichtigung der Herbar- und Sa- bankführung, Grüne Gentechnik, Nährstoffeffizienzuntersu- mensammlung (R. Schnee, Priv.-Doz. Dr. A. Börner, Dr. J. Keller, chungen (R. Schnee, Priv.-Doz. Dr. A. Börner, Dr. J. Kumlehn, Dr. K. Pistrick). Prof. Dr. N. von Wirén).

18. November 2010 Besuch von Dr. J.P. Sampoux, Leiter der Futterpflanzengenbank 2011 des INRA-Instituts Lusignan, Frankreich, Führung durch die Malchower Öl- und Futterpflanzen-Sortimente der IPK-Gen- 20. Januar 2011 bank und anschließende Kooperationsabsprachen für 2011 Besuch von Herrn C. Ullrich im Rahmen eines Schülerprakti- (S. Nehrlich, E. Willner). kums, Zarrendorf, Führung durch die Malchower Öl- und Fut- terpflanzen-Sortimente der IPK-Genbank (S. Nehrlich). 25. November 2010 Besuch einer Studentengruppe der Martin-Luther-Universität 31. Januar 2011 Halle-Wittenberg, 5 Personen, Vorstellung des Instituts, Besich- Besuch von Studenten der Hochschule für Technik und Wirt- tigung der Genbank und Campus-Führung (R. Schnee). schaft (HTW), Dresden, 10 Personen, Vorstellung des Instituts und der Genbank, Langzeitlagerung von Saatgut mit anschlie- 6. Dezember 2010 ßender Führung durch die Genbank, In vitro-Erhaltung und Besuch von Frau Heike Brehmer, Mitglied des Deutschen Bun- Kryo-Lagerung, Führung durch die Gewächshäuser der Gen- destages, und Herrn Detlef Gürth, CDU-Fraktion im Landtag bank (Dr. U. Lohwasser, Dr. J. Keller, J. Marlow). von Sachsen-Anhalt, Vorstellung des Instituts, Genom-basierte Ansätze in der Getreidezüchtung? Die Gerste-Genomsequenz 3. Februar 2011 ist in Sicht, Klimawandel und Ressourcenverknappung als Her- Besuch einer norwegischen Aktionskünstlerin, Führung durch ausforderung für die Pflanzenzüchtung: Das Beispiel Hybrid- die Groß Lüsewitzer Kartoffel-Sortimente der IPK-Genbank weizen, Rundgang und Besichtigung der LemnaTec-Anlage, (U. Behrendt, K.J. Dehmer, K. Göhrke). Sequenzierung (Prof. Dr. Th. Altmann, Dr. N. Stein, Dr. M. Gils, 8. Februar 2011 R. Schnee). Besuch von Schülern der 1. bis 4. Klasse der Grundschule Gaters- leben (Hortkinder), 20 Personen, Führung durch die Genbank, 14. Dezember 2010 Samenkühllager, Dreschhalle und durch die Gewächshäuser Besuch von Studenten der Hochschule Bremen, 45 Personen, der Genbank (R. Schnee, J. Marlow). Vorstellung des Instituts sowie der Aufgaben der Genbank, Vor- trag zu den Arbeiten der Taxonomie am IPK, Besichtigung der 10. Februar 2011 Herbar- und Samensammlung einschließlich des Samenkühlla- Besuch der Ministerin Prof. Birgitta Wolff in Begleitung von gers, Gewächshausbesichtigung (Priv.-Doz. Dr. A. Börner, Dr. F. Herrn Detlef Gürth (CDU), MdL Sachsen-Anhalt, Vorstellung des Blattner, Dr. K. Pistrick, J. Marlow). Instituts, allgemeine Diskussion mit den Mitgliedern des Direk- toriums, Vorführung der Mais-Pflanzenphänotypisierungsanla- ge (Prof. A. Graner, Prof. I. Schubert, Prof. T. Altmann, Prof. N. von Wirén, S.-A. Lorenz, R. Schnee).

236 1. März 2011 6. April 2011 Genbankführung für Dr. Bu-Jun Shi aus Adelaide, Australien Besuch von Mitarbeitern des Pfarrkonvents Salzlandkreis, 15 (Dr. U. Lohwasser). Personen, Vorstellung des Instituts, Diskussion zur Geschichte des IPK, zur Molekulargenetik und insbesondere zur Grünen 2. März 2011 Gentechnik (Prof. U. Wobus, R. Schnee). Besuch von Teilnehmern einer Lehrerfortbildung, 21 Personen, Vorstellung sowie Führung durch die Groß Lüsewitzer Kartof- 8. April 2011 fel-Sortimente der IPK-Genbank (U. Behrendt). Besuch von Schülern der 9. Klasse der Prof. Hans Lembke-Schu- le, Kirchdorf, 16 Personen, Vorstellung des Instituts, Führung 14. März 2011 durch die Malchower Öl- und Futterpflanzensortimente der Führung durch die Genbank für Prof. Director Dr. Qifa Zhang, IPK-Genbank (S. Nehrlich). Wuhan, China (Priv.-Doz. Dr. A. Börner). 20. April 2011 16. März 2011 Besuch der Mitglieder des VERN, Boitzenburger Land, 4 Perso- Besuch von Studenten der Universität Kiel, 6 Personen, Vor- nen, Begrüßung und Führung durch die Groß Lüsewitzer Kar- stellung des Instituts und Aufgaben der Genbank, Keimfähig- toffel-Sortimente der IPK-Genbank (U. Behrendt). keit, Saatgutaufreinigung/Ernteerfassung/Erntekontrolle mit Herbar und Samensammlung, Führung durch die Genbank 28. April 2011 (Dr. U. Lohwasser, C. Dittmann, S. Pistrick, A. Winger, M. Kotter, Besuch von Schülern der 6. Klasse der Prof. Hans Lembke-Schu- S. Schmidt, B. Harke, B. Schmidt. S. Eickmeier). le, Kirchdorf, 24 Personen, Begrüßung und Führung durch die Malchower Öl- und Futterpflanzensortimente derI PK-Genbank 18. März 2011 (S. Nehrlich). Besuch von Schülern der Takefu High Super Highschool, Fukui, Japan, 20 Personen, Vorstellung des Instituts, Strategien zur 29. April 2011 Verbesserung der pflanzlichen Ernährung, Führung durch die Besuch von rumänischen und deutschen Studenten im Rah- Genbank und Gewächshäuser, Vorführung der automatischen men eines Seminars „Jugendliche entdecken die Zukunft der Anlage zur Merkmalserfassung in Pflanzen (Prof. N. von Wirén, Landwirtschaft in der EU“, 15 Personen, Vorstellung des Insti- R. Schnee). tuts, Ährenmutationen und erhöhter Ertrag, Führung durch die Pflanzen-Samenbank, Besichtigung der LemnaTec-Anlage und 24. März 2011 der Feldflächen (R. Schnee,T . Seidensticker). Besuch von MinDirig’in Petra Steiner-Hoffmann, Frau Dr. Clau- dia Herok und Dr. Henk van Liempt vom BMBF sowie MinRat 3. Mai 2011 Thomas Reitmann und Frau Gisela Liepelt vom Kultusmini- Besuch von Absolventen, Wolfen, 11 Personen, Vorstellung des sterium des Landes Sachsen-Anhalt, Vorstellung des Instituts, Instituts, Diskussion zur Geschichte des IPK, zur Molekularge- Diskussionen zur Genomsequenzierung, Genidentifizierung netik und insbesondere zur Grünen Gentechnik (R. Schnee). mittels quantitativer genetischer Methoden, Analyse biologischer Prozesse/Genidentifizierung mittels systembiologischer 4. Mai 2011 Verfahren am Beispiel Samenfüllung, Charakterisierung von Besuch von Agraringenieurstudenten der Fachhochschule Kiel, Genbankmaterial, Erfahrungen und Stand der Entwicklung der 22 Personen, Vorstellung des Instituts, Identifizierung von Mar- Pflanzenphänotypisierung amI PK, Zielstellungen der IPK-Betei- kern für den Einsatz in Kreuzungen, Genbankführung, Besichti- ligung am DPPN, Deutsches Innovations-Netz zur Erschließung gung der Herbar-, Ähren- und Fruchtsammlung als Vergleichs- und Nutzung Pflanzengenetischer Ressourcen für die BioÖko- basis für die Bestimmung von pflanzengenetischen Ressourcen nomie, Besichtigung der Kulturpflanzenbank, Labor:B iochemi- (Dr. S. Kollers, Dr. U. Lohwasser, Dr. K. Pistrick, R. Schnee). sche Analytik, Sequenzierung, Vorführung Mais-Pflanzenphä- notypisierungsanlage (Prof. Dr. A. Graner, Prof. Dr. I. Schubert, 7. Mai 2011 Prof. Dr. T. Altmann, Prof. Dr. N. von Wirén, Dr. P. Schweizer, Besuch von Wasserbauingenieuren, 50 Personen, Vorstellung Dr. N. Stein, Dr. M. Röder, Dr. W. Weschke, R. Schnee). des Instituts, Führung durch die Genbank und Rundgang im IPK (R. Schnee). 31. März 2011 Besuch der ECPGR Umbellifer Working Group, 15 Personen, 11. Mai 2011 Vorstellung des Instituts, Besichtigung der Genbank und der Besuch der ehemaligen Leiterin Hella Brumme des Rosariums Herbar-, Ähren- und Fruchtsammlung, In vitro-Erhaltung und Sangerhausen mit Lehr- und Studienkollegen, 10 Personen, Cryo-Lagerung sowie der LemnaTec-Anlage und der Gewächs- Vorstellung des Instituts und der Genbank, Führung durch den häuser (Priv.-Doz. Dr. A. Börner, Dr. U. Lohwasser, Dr. J. Keller, Staudengarten (B. Schütze, R. Schnee). Dr. K. Pistrick, M. Oppermann, P. Schreiber, J. Marlow).

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11. Mai 2011 8. Juni 2011 Besucher eines Arbeitstreffens zum Projekt triploide Kamille, Besuch von Bürgermeistern aus Sachsen-Anhalt, 25 Personen, Vorstellung des Instituts und der Aufgaben der Genbank mit Vorstellung des Instituts, Führung durch die Genbank und Be- anschließender Genbankführung (Priv.-Doz. Dr. A. Börner). sichtigung der Freiflächen (P. Schreiber, R. Schnee).

17. Mai 2011 15. Juni 2011 Besuch von Frau Prof. Dr. Chris-Carolin Schön, LMU München, Besuch von Studenten der Universität Gießen, 30 Personen, Vorstellung der Aufgaben der Genbank mit anschließender Führung durch die Botanischen Vergleichssammlungen der Genbankführung (Priv.-Doz. Dr. A. Börner). Genbank, Erhaltung und Nutzbarmachung pflanzengenetischer Ressourcen für künftige Pflanzenzüchtung, Assoziations- 17. Mai 2011 genetik für Züchtung von Malzqualität bei Gerste, Feldführung Besuch von Mitgliedern des Plattdeutschen Vereins, Schwe- (Dr. K. Pistrick, Priv.-Doz. Dr. A. Börner, Dr. M. Röder). rin, 30 Personen, Vorstellung des Instituts, Führung durch die Malchower Öl- und Futterpflanzensortimente derI PK-Genbank 16. Juni 2011 (S. Nehrlich, E. Willner). Besuch von Studenten der Universität Hohenheim, 25 Perso- nen, Vorstellung der Aufgaben der Genbank, Informationen 19. Mai 2011 über die Aufgaben der Ag Genbankdokumentation und der Besuch einer Delegation aus Südkorea, 20 Personen, Vor- In vitro-Erhaltung und Cryo-Lagerung, Führung durch die Her- stellung des Instituts, Besichtigung der Genbank, Rundgang bar-Sammlungen und durch die Vermehrungsbestände der durch Labore und Gewächshäuser (Priv.-Doz. Dr. A. Börner, Genbank (Priv.-Doz. Dr. A. Börner, Dr. H. Knüpffer, Dr. J. Keller, R. Schnee). Dr. K. Pistrick, P. Schreiber).

20. Mai 2011 16. Juni 2011 Besuch von Studenten der Universität Rostock, Agrar- und Um- Besuch von Studenten der TU Dresden, 34 Personen, Vorstel- weltwissenschaftliche Fakultät/Agrobiotechnologie, 10 Perso- lung des Instituts und Rundgang auf dem Gelände, Besichti- nen, Führung durch die Groß Lüsewitzer Kartoffelsortimente gung der Kulturpflanzenbank und Führung durch die Labore der IPK-Genbank (Dr. K.J. Dehmer). der Ag Hefegenetik (Prof. G. Kunze, R. Schnee).

3. Juni 2011 17. Juni 2011 Besuch von Mitgliedern des Rotary Club München, 15 Perso- Besuch von Staatssekretär Marco Tullner, Ministerium für Wis- nen, Vorstellung des Instituts und der Aufgaben der Genbank, senschaft und Wirtschaft des Landes Sachsen-Anhalt, Vorstel- Führung durch das Genomzentrum und die Genbank (Prof. A. lung des Instituts, Gespräch mit den Mitgliedern des Direkto- Graner, Dr. habil. P. Schweizer, Priv.-Doz. Dr. A. Börner). riums, Besichtigung LemnaTec-Anlage (Prof. A. Graner, Prof. T. Altmann, Prof. I. Schubert, Prof. N. von Wirén, S.-A. Lorenz, K. 6. Juni 2011 Neumann, R. Schnee). Besuch von Schülern der 10. Klasse des Christophorus-Gym- nasiums Rostock, 17 Personen, Aufgaben der Groß Lüsewitzer 21. Juni 2011 Kartoffelsortimente der IPK-Genbank mit anschließender Füh- Besuch von Studenten der Fachhochschule Wismar, Umwelt- rung (Dr. K.J. Dehmer). und Verfahrenstechnik, 17 Personen, Führung durch die Mal- chower Öl- und Futterpflanzensortimente der IPK-Genbank 6.-9. Juni 2011 (S. Nehrlich, K. Ploen). Besuch von Dr. Grzegorz Gryziak, Gene Bank Manager, Natio- nal Center for Plant Genetic Resources, Radzików, Polen, Vor- 23. Juni 2011 stellung der Aufgaben der Genbank, Informationen über die Besuch von Geisenheimer Gartenbaustudenten, 12 Personen, Aufgaben der Ag Genbankdokumentation und der In vitro- Vorstellung des Instituts und der Aufgaben der Genbank, Be- Erhaltung und Cryo-Lagerung. Beseitigung der Herbar- und sichtigung des Samenkühllagers und der Herbar- und Samen- Samensammlung mit anschließender Genbankführung, Be- sammlung, Erläuterung der In vitro-Erhaltung und Cryo-Kon- sichtigung LemnaTec-Anlage und Gewächshaus (Priv.-Doz. Dr. servierung, Feldführung (Priv.-Doz. Dr. A. Börner, Dr. K. Pistrick, A. Börner, P. Schreiber, Dr. J. Keller, Dr. K. Pistrick, Dr. H. Knüpffer, Dr. J. Keller, P. Schreiber). M. Oppermann, C. Dittmann, J. Marlow, K. Krusch, K. Bollmann, M.A. Rehman Arif).

7. Juni 2011 Besuch von Kartoffelanbauern aus dem Kreis Kleve, 20 Perso- nen, Aufgaben der Groß Lüsewitzer Kartoffelsortimente der IPK-Genbank mit anschließender Führung (Dr. K.J. Dehmer).

238 23. Juni 2011 25.-29. Juli 2011 Besuch von Studenten der Berlin-Brandenburg School for Re- Besuch von S. Fourtier, Mitarbeiter der Futterpflanzengen- generative Therapies (BSRT), 21 Personen, Production of an- bank des INRA-Instituts Lusignan, Frankreich, Führung durch tibodies in plants, Introduction of IPK and tour through seed- die Malchower Öl- und Futterpflanzensortimente der IPK- bank, Plant evolution in time and space, Epigenetics in plants, Genbank, Kooperationsabsprachen für 2011/2012 (S. Nehrlich, Metabolomics and Iron acquisition, and transport in plants K. Ploen, E. Willner). (Priv.-Doz. Dr. U. Conrad, N. Nürk, A. Finke, T. Liiving, M. Franke, N. Schmid, R. Schnee). 28. Juli 2011 Besuch von Mitarbeitern der Fa. Saaten-Union, 20 Personen, 27. Juni 2011 Vorstellung der Genbank und Führung, Besichtigungen Sa- Besuch von Mitarbeitern der Ag Teilsammlungen Nord, Groß Lü- menkühllager, Herbar- und Samensammlung (Priv.-Doz. Dr. A. sewitz und Malchow, 20 Personen, Besichtigung der Genbank, Börner, Dr. K. Pistrick). Feld- und Gewächshausführung, Besichtigung der LemnaTec- Anlage (Priv.-Doz. Dr. A. Börner, Dr. A. Senula, P. Schreiber). 1. August 2011 Besuch von vietnamesischen Gastwissenschaftlern des JKI, 29. Juni 2011 2 Personen, Führung durch die Groß Lüsewitzer Kartoffels- Besuch von Kindern des Hortes Immenhus Tessin, 23 Personen, ortimente der IPK-Genbank und Erläuterung ihrer Aufgaben Aufgaben der Groß Lüsewitzer Kartoffelsortimente der IPK- (U. Behrendt, K. Diekmann). Genbank und anschließende Führung (U. Behrendt). 11. August 2011 1. Juli 2011 Besuch eines Wissenschaftlers der BOKU Wien, Führung durch Besuch einer Delegation der Academy of Agricultural Sciences, die Groß Lüsewitzer Kartoffelsortimente der IPK-Genbank und Nordkorea, 16 Personen, Vorstellung des Instituts und der Gen- Erläuterung ihrer Aufgaben (K. Diekmann). bank, Führung durch das Samenkühllager, Vorstellung der Ar- beit mit dem GBIS-System, Besichtigung der Vermehrungsflä- 3. September 2011 chen (Priv.-Doz. Dr. A. Börner, Dr. H. Knüpffer, M. Oppermann, Besuch im Rahmen eines Klassentreffens von Dr. H. Lellbach, P. Schreiber, R. Schnee). 8 Personen, Diedrichshagen, Führung durch die Malchower Öl- und Futterpflanzensortimente der IPK-Genbank und Erläu- 1. Juli 2011 terung (E. Willner). Besucher aus China, Afrika und Österreich vom Projekt Rei- chenstraße, Quedlinburg, 6 Personen, Vorstellung des Instituts, 3. September 2011 Besichtigung der Genbank, Forschung an resistentem Weizen Besuch von Mitgliedern des Landfrauenvereins Glasin, 15 Per- (R. Schnee, Dr. N. Sreenivasulu). sonen, Führung durch die Malchower Öl- und Futterpflanzen- sortimente der IPK-Genbank und Erläuterung (E. Willner). 4. Juli 2011 Besuch von Studenten der Universität Kassel-Witzenhausen, 5. September 2011 6 Personen, Aufgaben der Groß Lüsewitzer Kartoffelsortimente Besuch von Landwirten aus der oberbayerischen Region des der IPK-Genbank und anschließende Führung (Dr. K.J. Dehmer, Planungs- und Ausführungsbüros für angewandte Chaosfor- M. Vandrey). schung, Quantentechnologie, Geomantie und Galabau aus Bald- halm, 4 Personen, Vorstellung des Instituts und der Aufgaben 8. Juli 2011 der Genbank, Führung durch die Genbank und die Außenflä- Besuch von Dr. Günter Welz und Dr. Marcus Weidler, Bayer chen (Dr. U. Lohwasser). CropScience, Führung durch die Kulturpflanzenbank, Feldfüh- rung (Priv.-Doz. Dr. A. Börner, P. Schreiber). 14. September 2011 Besuch von Lehrern im Rahmen einer themengebundenen 8. Juli 2011 Lehrerfortbildung des Landesinstituts für Schulqualität und Besuch von Anbauberatern der Landwirtschaftskammer NRW, Lehrerbildung Sachsen-Anhalt, Halle/S., 30 Personen, Ge- 30 Personen, Vorstellung des Instituts, Führung durch die wächshaus-Rundgang: technische Lösungen für die Pflanzen- Genbank und die taxonomischen Sammlungen, Feldführung forschung, Genbank-Führung, Besichtigung Staudengarten (R. Schnee, P. Schreiber, M. Grau). (Priv.-Doz. Dr. A. Börner, E. Geyer).

8. Juli 2011 15. September 2011 Besuch von Weizenzüchtern, Institute of Food Crops, Jiangsu Besuch von zwei 9. Klassen des Weinberggymnasiums Klein- Academy of Agricultural Sciences, Nanjing, China, 3 Personen, machnow, 30 Personen, Führung durch die Groß Lüsewitzer Vorstellung der Aufgaben der Genbank mit anschließender Kartoffelsortimente der IPK-Genbank und Erläuterung der Auf- Feldführung im Getreidesortiment (M. Grau). gaben (Dr. K.J. Dehmer).

239 Öffentlichkeitsarbeit/Public Relations

16. September 2011 28. September 2011 Besuch von Mitarbeitern der Mitteldeutschen Baumschulen, Besuch im Rahmen eines Klassentreffens von G. Bachler, Nien- Reinstedt, 6 Personen, Vorstellung des Instituts, Rundgang Ge- dorf, 10 Personen, Führung durch die Malchower Öl- und Fut- wächshauskomplex Genetik und Gelände, Führung durch die terpflanzensortimente der IPK-Genbank (V. Miehe). Genbank: Herbar-, Ähren- und Samensammlung, Samenkühl- lager (R. Schnee). 28.-29. September 2011 Besuch einer Mitarbeiterin des National Agriculture Institute 24. September 2011 Kairo, Ägypten, Führung durch die Malchower Öl- und Futter- Besichtigung der Botanischen Vergleichssammlungen durch pflanzensortimente der IPK-Genbank (S. Nehrlich, E. Willner). S. Jantassov, Kaskh Research Institute of Potato and Vegetable Production (Dr. K. Pistrick). 29. September 2011 Besuch von Dr. Fransisca Tan, CEO Gudang Garam, Indonesia- 27. September 2011 based cigarette producer, 2 Personen, Vorstellung des Instituts, Besuch von Fachschullehrern aus China, 7 Personen, Vorstel- Besichtigung der Gewächshausanlagen und Labore, Führung lung des Instituts, Ug99 – Globalisierte Gefahr, Führung Gen- durch die Genbank (R. Schnee). bank – insbesondere Weizenkollektion (Dr. M. Röder, Priv.-Doz. Dr. A. Börner, R. Schnee). 4.-6. Oktober 2011 Besuch von drei 4. Klassen der Grundschule „John Brinckman“, 27. September 2011 Rostock, ca. 70 Personen, Vorstellungen und Führung der Groß Besuch von Mitgliedern des Landwirtschaftsausschusses im Lüsewitzer Kartoffelsortimente der IPK-Genbank (U. Behrendt, Landtag von Südtirol, 10 Personen, Vorstellung des Instituts, K. Göhrke, K. Löschner, M. Vandrey). Grundlagen der Gentechnik, Neue Ansätze zur Züchtung er- tragreicherer Weizensorten, Führung durch die Genbank (Dr. J. 6. Oktober 2011 Kumlehn, R. Schnee). Besuch von Mitarbeitern des Instituts für Geobotanik, Halle/S., 2 Personen, Vorstellung der Aufgaben der Genbank und der 28. September 2011 Arbeitsgruppe Experimentelle Taxonomie, Besichtigungen des Besuch von Mitarbeitern der Justizbehörden des Landes Samenkühllagers und der Herbar- und Samensammlung (Dr. F. Sachsen-Anhalt, 10 Personen, Vorstellung des Instituts, Rund- Blattner, Dr. U. Lohwasser). gang: Campus, Felder, Genbank, Labore, Gewächshaus PGRC (R. Schnee).

240 10. Oktober 2011 9. November 2011 Besuch von Studenten der Hochschule Bremen, 30 Personen, Besuch von Studenten der Otto-von-Guericke-Universität Vorstellung des Instituts und der Aufgaben der Genbank, Vor- Magdeburg, Fachrichtung Biosystemtechnik, 14 Personen, Vor- trag zu den Arbeiten der Taxonomie am IPK, Besichtigung der stellung des Instituts und der Arbeitsgruppe Systembiologie, Herbar- und Samensammlung, Samenkühllager sowie Ge- Führung durch die Genbank, die Gewächshäuser am Genom- wächshausbesichtigung (Priv.-Doz. Dr. A. Börner, Dr. F. Blattner, zentrum und die Genetik (Dr. B. Junker, R. Schnee). Dr. K. Pistrick, J. Marlow). 18. und 25. November 2011 13. Oktober 2011 Besucher des Freiwilligen Ökologischen Jahres (FÖJ), Jugend- Besuch von Dr. Mirzaei, Dr. Jafari und Dr. Ashani, Research werk Aufbau Ost e.V., Berlin, je 30 Personen, Vorstellung des Institute for Forest and Rangelands Teheran, Iran, 4 Personen, Instituts, Biodiversität in der Genbank, Grüne Gentechnik, (Priv.- Vorstellung des Instituts, Besichtigung der Genbank und der Doz. Dr. A. Börner, Dr. G. Hensel, Dr. J. Kumlehn, R. Schnee). Botanischen Vergleichssammlungen, In vitro-Erhaltung und Cryo-Lagerung (Prof. Dr. A. Graner, Dr. K. Pistrick, Dr. J. Keller, 22. November 2011 R. Kurch, R. Schnee). Besucher im Rahmen des Berufsorientierungstages des Grü- nen Labors, Gatersleben, 7 Personen, Gewächshausrundgang 20. Oktober 2011 (E. Geyer). Besuch einer Praktikantin der NPZ, Führung durch die Malcho- wer Öl- und Futterpflanzensortimente der IPK-Genbank (E. Will- 1. Dezember 2011 ner). Besuch von Schülern des John-Brinckman-Gymnasiums, Gü- strow, 16 Personen, Führung durch die Groß Lüsewitzer Kartof- 26. Oktober 2011 felsortimente der IPK-Genbank (U. Behrendt, M. Vandrey). Vorlesung für Mitarbeiter des IPK im Rahmen der Innerbe- trieblichen Qualifizierung: „Sequenziertechniken: Methodische 8. Dezember 2011 Grundlagen, aktuelle Anwendungen und zukünftige Entwick- Besuch von Schülern des Käthe-Kollwitz-Gymnasiums, Rostock, lungen“ (Dr. L. Altschmied). 15 Personen, Führung durch die Groß Lüsewitzer Kartoffelsorti- mente der IPK-Genbank (U. Behrendt, Dr. K.J. Dehmer). 3. November 2011 Besuch von Schülerinnen im Rahmen einer Ausbildung zur 14. Dezember 2011 biologisch-technischen Assistentin, Bad Berleburg, 2 Personen, Besuch von Studenten des Kurses „Bioethik“ der Martin-Luther- Vorstellung des Instituts und der Arbeitsgruppe Phytoantikör- Universität, Halle/S., 15 Personen, Rundgang durch das Institut, per, Spinnenseidenproteine (R. Schnee, N. Weichert). Einführung zur Anwendung von Gewebekulturen in der Pflan- zenanzucht, Grüne Gentechnik, Rundgang durch Labore der Arbeitsgruppen In vitro-Erhaltung und Cryo-Lagerung sowie Pflanzliche Reproduktionsbiologie (Dr. J. Keller, Dr. J. Kumlehn, Dr. G. Hensel, R. Schnee).

241 Pressemitteilungen/Press Releases

Pressemitteilungen/Press Releases

2010 4. März 2011 20. Januar 2010 Saat für die Zukunft. Neuer WissenschaftsCampus „Pflanzen- Langjähriger Administrativer Leiter des IPK Gatersleben schei- basierte Bioökonomie“. det aus dem Institut. (Gemeinsame Pressemitteilung des Kultusministeriums des Landes Sachsen-Anhalt, der Martin-Luther-Universität Halle- 23. März 2010 Wittenberg und der Leibniz-Gemeinschaft). Erster Wricke-Preis geht an Gaterslebener Forscher. 15. März 2011 11. Mai 2010 Feldzerstörer scheitern erneut vor Gericht. Agrar-Staatssekretär besuchte das IPK und das Schülerlabor. Bundesgerichtshof bestätigt Urteil des OLG Naumburg.

14. Juni 2010 13. April 2011 IPK Gatersleben nun auch offiziell familienfreundlicher Arbeit- Einladung zur Gaterslebener Begegnung XII, vom 12. bis 14. geber. Mai 2011, zum Thema „Wachstum und Reifung in Natur und Gesellschaft“. 8. September 2010 3. Gaterslebener Gespräch „Globale Aspekte der Grünen Gen- 25. Mai 2011 technik“ am 17. September. Biotech-Campus Gatersleben lädt zum Tag der offenenT ür ein.

9. September 2010 30. Mai 2011 IPK Gatersleben präsentiert Kürbisse auf LAGA 2010 in Aschers- Vier Feldzerstörer fechten mildes Urteil des Amtsgerichtes leben. Aschersleben an. Berufungsverhandlung vor dem Landgericht Magdeburg am 4. Oktober 2010 1. Juni. Neue Resistenzgene gegen Weizenmehltau in alten Landras- sen entdeckt. 14. Juni 2011 8. Gaterslebener Forschungspreis geht an Züricher Nachwuchs- Weitere 7.000 Samen gehen auf die Reise gen Nordpol. wissenschaftlerin. 20. Juni 2011 22. November 2010 Eine Woche Hybridzüchtung. Zukunft der Pflanzenforschung im Visier. 10. Gatersleben Re- IPK Gatersleben ist Gastgeber für internationale Pflanzenzüchter. search Conference. 14. Juli 2011 8. Dezember 2010 Weshalb die doppelte Hälfte besser ist als das ursprüngliche Mehr Schlagkraft für Forschung zur Kulturpflanze in der Region. Ganze. (Gemeinsame Pressemitteilung vom IPK Gatersleben und dem Pflanzenforscher entdecken Geheimnis zur Eliminierung von JKI Quedlinburg anlässlich der Unterzeichnung eines Koopera- Chromosomen. tionsvertrages). 22. Juli 2011 Berufung der Feldzerstörer von Gatersleben scheitert vor dem Magdeburger Landgericht. 2011 Strafkammer des Landgerichts Magdeburg verurteilt Feldzer- störer in Revisionsverfahren. 10. Februar 2011 Erster Stopp: Leibniz-Institut für Pflanzengenetik und Kultur- 5. Dezember 2011 pflanzenforschung (IPK). Forschungspreise gehen an Daniela Christiane Dieterich, Gott- Die Kultusministerin des Landes, Frau Prof. Dr. Birgitta Wolff, be- hard Kunze und Niels Angermüller. suchte Leibniz-Einrichtungen in Sachsen-Anhalt.

242 Presse- und Medienarbeit/Contributions in Press and Media soweit erfasst/as registered

2010 31. Mai 2010 MZ-Marktplatz, Nr. 22, „IPK wird für seine Personalpolitik ge- 25. Januar 2010 ehrt“ (R. Schnee). Mitteldeutsche Zeitung Aschersleben, „Wechsel an der Füh- rungsspitze“ – Langjähriger Administrativer Leiter des IPK geht 9. Juni 2010 in den Ruhestand (B. Eise, S.-A. Lorenz). Mitteldeutsche Zeitung, „Wissenschaft gibt tiefe Einblicke“ ‑ Tag der offenenT ür und Fest der Begegnung laden am Sonnabend 28. Januar 2010 ein (R. Schnee). Mitteldeutsche Zeitung, „Forscher warten auf EU-Entschei- dung“ ‑ CDU-Landtagsabgeordneter Detlef Gürth besucht den 14. Juni 2010 Biotechnologiestandort Gatersleben (Prof. Dr. A. Graner). Mitteldeutsche Zeitung, Aschersleben, „Die Welt der Wissen- schaft zum Anfassen und Probieren“ – Zahlreiche Besucher ka- 24. März 2010 men zum Tag der offenenT ür und zum Begegnungsfest Volksstimme, „Wricke-Forschungspreis erstmals verliehen“ (Prof. Dr. A. Graner, R. Schnee). (Dr. N. Stein). 22. Juni 2010 22. April 2010 Mitteldeutsche Zeitung, Aschersleben, „Leibniz-Institut erhält DIE ZEIT, Nr. 17, „Mendels Kinder“ – Junge Pflanzenforscher ha- offiziell familienfreundliches Prädikat“ – Vereinbarkeit von Fa- ben es oft mit Gentechnik zu tun, aber nur wenige bauen trans- milie und Beruf stehen im Mittelpunkt (Prof. Dr. A. Graner). gene Pflanzen (T. Guse, Prof. Dr. A. Graner). 6. Juli 2010 1. Mai 2010 ARD/MDR, Interview, „Forschung am IPK“ (R. Schnee). Mitteldeutsche Zeitung, Aschersleben, „Erster Wricke-Preis geht an Gaterslebener Forscher“ (Dr. N. Stein, R. Schnee). 8. Juli 2010 Zeitschrift „K+S Information“, Kassel, „Die Welt braucht unsere 4. Mai 2010 Düngemittel“ (anlässlich Fachtagung „Megatrend Agrarroh- Mitteldeutsche Zeitung, Magdeburg, „Genweizen-Prozess: stoffe“) (Prof. Dr. A. Graner). Neue Vorwürfe vor neuer Runde“ – Gentechnik-Gegner sehen Versuchsgenehmigung als Farce (Prof. Dr. A. Graner). 20. Juli 2010 Mitteldeutsche Zeitung, Halle/S., Interview, „Bedeutung der 17. Mai 2010 Züchtung, Landwirtschaft und Biodiversität“ (R. Schnee). Deutschlandfunk Magdeburg, Interview, „Rechtsstreit Feldzer- störung“ (Prof. Dr. A. Graner). 20. Juli 2010 Mitteldeutsche Zeitung, „Junge Wissenschaftler machen sich 22. Mai 2010 auf die Spur der Nabelschnur“ – Doktoranden des Gatersle- HARZER Kreisblatt, Nr. 5, „Tag der offenen Tür im Institut für bener Leibniz-Instituts gehen auf Exkursion und untersuchen Pflanzengenetik“ (N. Wahle). ungewöhnliche Firmengeschichten in der Region (M. Mau, L. Gerson, H. Wolf von der BIO Mitteldeutschland GmbH). 23. Mai 2010 Generalanzeiger, „Agrar-Staatssekretär besuchte das IPK und 21. Juli 2010 das Schülerlabor“ (Prof. Dr. A. Graner). Mitteldeutsche Zeitung, „Kritik an Patent auf Brokkoli“ (Prof. Dr. A. Graner). 26. Mai 2010 NDR Hamburg, Interview für W. wie Wissen, „Biodiversität in 22. Juli 2010 Kleingärten am Beispiel der Kulturpflanzen“ (Prof. Dr. A. Graner). nufan-Film, Berlin, Beiträge für biotechnologie.tv, Interview, „Zehn Jahre Arabidopsis-Genom; Genbank Gatersleben; Gerste- Genom: Erstmals deutsche Koordinierung einer Kulturpflan- zensequenzierung“ (Prof. Dr. Th. Altmann, Prof. Dr. A. Graner, Dr. N. Stein).

243 Presse- und Medienarbeit/Contributions in Press and Media

22. Juli 2010 1. Oktober 2010 Mitteldeutsche Zeitung, „Kleine Bauarbeiter probieren sich auf Mitteldeutsche Zeitung, Aschersleben, „Glaubt an Aufrichtig- neuer Baustelle aus“ – Die Gaterslebener Kindertagesstätte keit“ – Zusammenhalten – Zukunft gewinnen (Dr. N. Sreeniva- überrascht mit neuem Angebot (S.-A. Lorenz). sulu).

19. August 2010 15. Oktober 2010 Mitteldeutsche Zeitung, „Globale Aspekte der Gentechnik“ Mitteldeutsche Zeitung, Aschersleben, „Verhandlung geht in (R. Schnee). eine dritte Runde“ – Feldzerstörer-Angeklagte lehnen eine Ver- urteilung mit einer geringen Geldstrafe ab (Dr. W. Weschke). 26. August 2010 WDR, „Das Wunder von Köln“, Materialbereitstellung für Film- 27. Oktober 2010 aufnahmen (Dr. K. Pistrick). mdr aktuell, Interview, „Fragen zu Untersuchungen und Expe- rimenten am IPK, Potenziale für die Ernährungssituation bei 3. September 2010 uns und möglicherweise in der ganzen Welt“ (Dr. J. Kumlehn, dapd Nachrichtenagentur GmbH, Fotoaufnahmen von For- R. Schnee). schungsarbeiten am IPK anlässlich des Gründungsjubiläums des InnoPlanta e.V. (R. Schnee). 24. November 2010 Mitteldeutsche Zeitung, Aschersleben, „Pflanzenforscher aus 14. September 2010 22 Nationen“ – Die Gaterslebener Research Conference be- Mitteldeutsche Zeitung, Aschersleben, „Leibniz-Institut zeigt schäftigt sich mit Genomforschung (R. Schnee, Dr. N. Stein). Kürbisse“ (R. Schnee). 8. Dezember 2010 14. September 2010 WDR Köln, Interview, „Welche Methoden werden eingesetzt, um Mitteldeutsche Zeitung, Aschersleben, „Sportler setzen Zeichen Getreidesorten auf neue klimatische Bedingungen vorzuberei- gegen Gewalt“ – Traditionelles Fußballspiel findet in Gatersle- ten und welche Erfolge man mit herkömmlichen Züchtungs- ben bereits seit zehn Jahren statt (R. Schnee). methoden haben kann, wenn es um Resistenzen gegen Pilze o.ä. geht? Welche Rolle wird die Gentechnik in den nächsten 14. September 2010 Jahren in der Getreidezucht einnehmen?“ (Prof. Dr. A. Graner, Mitteldeutsche Zeitung, Aschersleben, „Prozessauftakt gegen Dr. M. Röder). Feldzerstörer“ (R. Schnee).

15. September 2010 Mitteldeutsche Zeitung, Aschersleben, „Die Grüne Gentechnik 2011 aus globaler Sicht“ – Morgen Abend beginnt das 3. Gaterslebe- ner Gespräch (R. Schnee). 25. Januar 2011 MDR, LexiTV, Leipzig, Dreharbeiten über das IPK zum Thema 16. September 2010 „Vererbung“ (Priv.-Doz. Dr. A. Börner, Dr. U. Lohwasser, P. Schrei- Mitteldeutsche Zeitung, Aschersleben, „Kürbisse mit Feigen- ber, J. Marlow, R. Schnee). blatt und Warze“ – Gaterslebens Leibniz-Institut präsentiert Halloweenklassiker in allen Formen und Farben (B. Schmidt). 3. Februar 2011 radioeins, Telefoninterview, „Archivierung von Pflanzen“ (Prof. 17. September 2010 Dr. A. Graner). MDR Figaro, Das Kulturradio des Mitteldeutschen Rundfunks, Interview, „Die positiven Seiten der Grünen Gentechnik und 9. Februar 2011 welche rationalen Argumente gibt es“ (R. Schnee, Prof. Dr. A. Mitteldeutsche Zeitung, Aschersleben, „Zwölfte Auflage der Graner). Gaterslebener Begegnung“ (R. Schnee).

18. September 2010 12. Februar 2011 Mitteldeutsche Zeitung, Aschersleben, „Knorrige Bäume und Mitteldeutsche Zeitung, Aschersleben, „Kultusministerin be- leuchtende Weizenähren“ – Eine Kunstausstellung eröffnet das sucht das Leibniz-Institut“ (Prof. Dr. A. Graner). Gaterslebener Gespräch (Prof. Dr. Anna M. Wobus, Prof. Dr. U. Wobus, Prof. Dr. A. Graner, R. Schnee, K. Menzel).

244 7. März 2011 8. Juni 2011 Mitteldeutsche Zeitung, Aschersleben, „Kultureller Atem der Bayerisches Fernsehen, Redaktion Unser Land, Beitrag über die Region“. Der Gaterslebener Kulturverein ist 20 Jahre alt und Genbank in Gatersleben, Sortenvielfalt und Möglichkeiten der schaut auf über 100 Veranstaltungen zurück (Dr. H. Knüpffer, grünen Gentechnik, Fragen zu GVO (Priv.-Doz. Dr. A. Börner, K. Menzel). Prof. Dr. T. Altmann).

11. März 2011 10. Juni 2011 Leipzig School of Media, Telefoninterview, „Kommunikation Mitteldeutsche Zeitung, Aschersleben, „Hefe entpuppt sich als und Akzeptanz grüner Gentechnik“ (R. Schnee). vielseitiges Talent“. Gaterslebener Wissenschaftler entwickeln Tests für Östrogen und Hormone (Prof. Dr. G. Kunze, Dr. K. Flor- 17. März 2011 schütz, Dr. M. Giersberg). Mitteldeutsche Zeitung, Aschersleben, „Schicksal der Partner geht nah“. Das IPK sorgt sich um japanische Institute (Prof. Dr. 15. Juni 2011 A. Graner, Prof. Dr. N. von Wirén). Mitteldeutsche Zeitung, Aschersleben, „Neue Samen für Gen- bank am Nordpol“ (R. Schnee). 15. April 2011 Mitteldeutsche Zeitung, Aschersleben, „Wachstum in Natur 17. Juni 2011 und Gesellschaft“. Die zwölfte Gaterslebener Begegnung ver- Volksstimme, „Gatersleber Genbank geht auf Nummer sicher“. eint Wissenschaftler, Künstler und Politiker (R. Schnee). Pflanzensamen werden zusätzlich auf Spitzbergen eingelagert (Priv.-Doz. Dr. A. Börner). 14. Mai 2011 Mitteldeutsche Zeitung, Aschersleben, „Blumiger Schatz aus 21. Juni 2011 der Kantine“. Leibniz-Institut eröffnete Ausstellung (Prof. Dr. U. Mitteldeutsche Zeitung, Aschersleben, „Leibniz-Institut be- Wobus). herbergt Züchter aus aller Welt“. In dieser Woche veranstaltet amerikanische Universität in Gatersleben ein Fortbildungspro- Mai 2011 gramm (R. Schnee). Poeler Inselblatt, „Tag der offenenT ür – Der Wissenschaftsstand- ort Malchow öffnet seineT ore“ (S. Nehrlich). 21. Juni 2011 Prof. Dr. H. Spohler, Hochschule für Technik und Wirtschaft, 18. Mai 2011 Berlin, Fotoaufnahmen vom IPK zum Buch- und Ausstellungs- LaborJournal, Telefoninterview „Pflanzenvielfalt aus dem Tief- projekt „Der dritte Tag“ (Priv.-Doz. Dr. A. Börner, S. Pistrick, kühlschrank“ (Prof. Dr. A. Graner). A. Winger, I. Mücke, Dr. J. Kumlehn, R. Schnee).

25. Mai 2011 24. Juni 2011 Mitteldeutsche Zeitung, Aschersleben, „Pflanzenvielfalt zum Mitteldeutsche Zeitung, Aschersleben, „Von Adler-Imitat, Hand- Staunen, Anfassen und zum Verkosten“. Gaterslebens Biotech- arbeit und Unkrautbildern“. Gaterslebener Institut ist Gastgeber nologie-Campus lädt Sonnabend zum Tag der offenen Tür ein für Pflanzenzüchter-Weiterbildung (Priv.-Doz. Dr. A. Börner). (R. Schnee). 29. Juni 2011 27. Mai 2011 Volksstimme, „Gentechnik und Erhalt der Vielfalt gehen Hand in Mitteldeutsche Zeitung, Aschersleben, „Hefen, die zum Bac- Hand“ (R. Schnee). ken nicht taugen“. Zum Tag der offenenT ür (Prof. Dr. G. Kunze, R. Schnee). 1. Juli 2011 Volksstimme, „Auf Vermehrungsflächen der Genbank beginnt 31. Mai 2011 Getreideernte“ (Priv.-Doz. Dr. A. Börner). Mitteldeutsche Zeitung, Aschersleben, „Das Ei der Pflanzen unter dem Mikroskop gesehen“. Im Gaterslebener Institut für 7. Juli 2011 Pflanzengenetik und Kulturpflanzenforschung konnten die Mitteldeutsche Zeitung, Aschersleben, „Wenn Gene einfach Gäste so einiges sehen, schmecken und fühlen (Dr. H. Bäum- blaumachen“. IPK-Arbeitsgruppe betreibt Grundlagenfor- lein, Dr. U. Lohwasser, I. Mücke). schung für die Pflanzenzucht (Dr. M.F. Mette, Dr. M. Kuhlmann).

245 Presse- und Medienarbeit/Contributions in Press and Media

8. Juli 2011 15. Oktober 2011 Mitteldeutsche Zeitung, Aschersleben, „Sichere Impfstoffe Mitteldeutsche Zeitung, Aschersleben, „Zwischen Lichtjahren aus der Region“. IPK-Nachwuchskräfte sind auf Fachexkursion und Nanometern“. Illustratorin Svetlana Kilian stellt im Leibniz- (S. Witter vom PhD Student Board, U. Pigla von BIO Mittel- Institut aus (K. Menzel). deutschland GmbH). 13. November 2011 15. Juli 2011 Frankfurter Allgemeine Sonntagszeitung, Wissenschaft, Nr. 45, Frau im Spiegel, Telefoninterview „Ist Genfood gefährlich?“ „Saatgut für die Ururenkel“ (Priv.-Doz. Dr. A. Börner). (Prof. Dr. A. Graner). 5. Dezember 2011 26. Juli 2011 Mitteldeutsche Zeitung, Aschersleben, „Gaterslebener wird mit Mitteldeutsche Zeitung, Aschersleben, „Berufung der Feldzer- Forschungspreis geehrt“ (R. Schnee). störer scheitert“ (R. Schnee).

12. Oktober 2011 Mitteldeutsche Zeitung, Aschersleben, „Wissenschaft trifft wieder auf Kultur“. Konzert und Vernissage im IPK (K. Menzel).

246 Messen und Ausstellungen/Fairs and Exhibitions

2010 29. August 2010 Auf der Ausstellung BioErleben 2010 in Warnemünde präsen- 23. bis 26. März 2010 tierte die Arbeitsgruppe Teilsammlungen Nord des IPK das Das IPK beteiligte sich im Rahmen des Gemeinschaftsstandes Groß Lüsewitzer Kartoffel-Sortiment (Dr. K.J. Dehmer, U. Beh- „Forschung für die Zukunft“ mit einem Exponat zum Thema rendt). „Biosensoren zum Nachweis östrogen wirksamer Substanzen“ (Dr. M. Giersberg, T. Gerlach) an der ANALYTICA 2010 in Mün- 15. September 2010 chen. Das IPK war auf der Landesgartenschau 2010 (LAGA) in Aschers- leben vertreten und gestaltete mit einer Präsentation von zahl- 8. Mai 2010 reichen Kürbissen das Thema „Die bunte Welt der Kürbisse“ an- Mitarbeiter der Teilsammlungen Malchow der IPK-Genbank schaulich (Dr. U. Lohwasser, B. Schmidt, S. Eickmeier). präsentierten sich auf dem 8. Landesrapsblütenfest in Stern- berg, Mecklenburg-Vorpommern, mit einem Ausstellungsstand 16. bis 19. September 2010 „Pflanzenvielfalt – Anbau und Erhaltung in der Genbank“. Die Mitarbeiter der Teilsammlungen Nord nahmen an der Mecklen- Veranstaltung wurde von etwa 1000 Gästen besucht (V. Miehe, burger Landwirtschaftsausstellung (MeLa) teil und präsentier- S. Hünmörder). ten sich auf dem Gemeinschaftsstand des Rates für Agrarwis- senschaften Mecklenburg-Vorpommern mit der Ausstellung 7. bis 8. Juni 2010 „Teilsammlungen Nord der IPK-Genbank“ (U. Behrendt, Dr. K.J. Beteiligung des IPK an der NAROSSA 2010 (Nachwachsende Dehmer, K. Göhrke, S. Pelikan, K. Ploen, E. Willner). Rohstoffe und Pflanzenbiotechnologie) in Magdeburg zum Thema „Plant breeding for non food application“ (Dr. K. Flor- 5. bis 7. Oktober 2010 schütz). Mitarbeiter des IPK präsentierten sich erneut im Rahmen des Gemeinschaftsstandes „Forschung für die Zukunft“ mit einem 9. Juni 2010 Exponat zum Thema „Auf den Spuren von Viren und Pilzen“ auf Das IPK war auf der Landesgartenschau 2010 (LAGA) in Aschers- der BIOTECHNICA 2010 in Hannover (Dr. K. Florschütz). leben vertreten und gestaltete einen Stand zu Doldengewäch- sen (Dr. U. Lohwasser, B. Schmidt, R. Kurch, M.-L. Graichen, Priv.- 14. Oktober 2010 Doz. Dr. A. Börner, F. Schröder). Bereits zum dritten Mal riefen der Wirtschaftsclub Aschersleben e.V. und die Stadt Aschersleben zu einem Berufsorientierungs- 12. bis 13. August 2010 tag auf, an dem auch das Institut teilnahm. Es wurde vor allem Das IPK beteiligte sich an einer gemeinsamen Veranstaltung den Sekundarschülern die Möglichkeit gegeben, sich beruflich von Lehr- und Forschungseinrichtungen in Sachsen-Anhalt, zu orientieren und über Praktikums- und Ausbildungsplätze zu die vom Julius Kühn-Institut in Quedlinburg unter dem Motto informieren (J. Marlow). „Biologische Vielfalt bei Pflanzen“ durchgeführt wurde. Mitar- beiter präsentierten zum Thema „Biodiversität zum Probieren“ Doldenblütler mit einer anschließenden Teeverkostung (Dr. U. Lohwasser, B. Schmidt, R. Kurch, M.-L. Graichen).

247 Messen und Ausstellungen/Fairs and Exhibitions

2011 28. August 2011 Präsentation der Groß Lüsewitzer Kartoffel-Sortimente auf der 21. bis 30. Januar 2011 Ausstellung BioErleben 2011 in Warnemünde (über 25.000 Be- Grüne Woche Berlin: Beteiligung der Arbeitsgruppe Teilsamm- sucher; U. Behrendt). lungen Nord/Standort Groß Lüsewitz der IPK-Genbank am Ausstellungsstand des BMBF (insgesamt ca. 415.000 Besucher; 4. September 2011 Dr. K.J. Dehmer, K. Diekmann). Tag der offenen Tür am Standort Groß Lüsewitz der Arbeits- gruppe Teilsammlungen Nord der IPK-Genbank mit Führungen 7. Mai 2011 und Erläuterungen zu den Groß Lüsewitzer Kartoffel-Sortimen- 9. Landes-Rapsblütenfest in Sternberg: Beteiligung der Arbeits- ten im Rahmen des Park- und Seefestes Groß Lüsewitz. gruppe Teilsammlungen Nord/Standort Malchow der IPK-Gen- bank mit einem Ausstellungsstand „Pflanzenvielfalt – Anbau 15.-18. September 2011 und Erhaltung in der Genbank“ (ca. 1.000 Besucher; K. Ploen, Mitarbeiter der Arbeitsgruppe Teilsammlungen Nord der IPK- S. Nehrlich). Genbank beteiligten sich auf der Mecklenburger Landwirt- schaftsausstellung (MELA) am Gemeinschaftsstand des Rates 14. Mai 2011 für Agrarwissenschaften Mecklenburg-Vorpommern. Diese 5. Poeler Rapsblütenfest in Kirchdorf: Beteiligung der Arbeits- Veranstaltung verzeichnete etwa 66.000 Besucher (U. Beh- gruppe Teilsammlungen Nord/Standort Malchow der IPK- rendt, Dr. K.J. Dehmer, K. Diekmann, K. Löschner, S. Nehrlich, Genbank mit einem Ausstellungsstand „Pflanzenvielfalt – An- S. Pelikan, K. Ploen, E. Willner). bau und Erhaltung in der Genbank“ (ca. 1.000 Gäste; V. Miehe, E. Willner). 11. bis 13. Oktober 2011 Mitarbeiter aus dem IPK beteiligten sich am Gemeinschafts- 16. März 2011 stand „Forschung für die Zukunft“ mit Exponaten zum Thema Das Institut beteiligte sich am Tag der Berufe, der im Biotech- „Biologische Sensorsysteme“ auf der BIOTECHNICA 2011 in Gründerzentrum durchgeführt wurde. In diesem Zusammen- Hannover (Dr. K. Florschütz, Dr. M. Giersberg). hang wurden die Ausbildungsberufe Biologielaborant/-in und Gärtner/-in vorgestellt.

7. bis 9. Juni 2011 An der Veranstaltung Sensor + Test 2011, die in Nürnberg stattfand, beteiligten sich Mitarbeiter des IPK mit einem Poster „On- Chip-Nachweis von phytopathogenen RNA-Viren und miRNA mittels Oberflächenplasmonenresonanz“ (Dr. K. Florschütz, A. Schröter, Prof. Dr. G. Kunze).

248 Gremien und Mitarbeiter/-innen in speziellen Funktionen/ Boards of the IPK and Employees with Special Responsibilities

Der Stiftungsrat überwacht die Geschäftsführung des Direk- Mitglieder des Direktoriums toriums, überprüft die Wirtschaftsführung, genehmigt die Jah- resrechnung und erteilt Entlastung für das jeweils abgelaufene Prof. Dr. Andreas Graner, Geschäftsführender Direktor und Haushaltsjahr. Leiter der Abteilung Genbank, Sybille-Andrea Lorenz, Administrative Leiterin und Leiterin Mitglieder des Stiftungsrates der Abteilung Verwaltung und Zentrale Dienste, Prof. Dr. Ingo Schubert, Leiter der Abteilung Cytogenetik und MinDirig Dr. Joachim Welz, MK LSA, Magdeburg, (Vorsitz bis Genomanalyse, November 2011), Prof. Dr. Thomas Altmann, Leiter der Abteilung Molekulare RD Peter Hassenbach. BMBF, Bonn, (stellv. Vorsitz bis Septem- Genetik, ber 2010), Prof. Dr. Nicolaus von Wirén, Leiter der Abteilung Physiologie Dr. Henk van Liempt, BMBF, Bonn, (stellv. Vorsitz seit März und Zellbiologie. 2011), Martin Köhler, BMELV, Bonn, (bis Oktober 2010), MinRat Thomas Reitmann, MK LSA bis April 2011; MW LSA seit Der Wissenschaftliche Beirat berät den Stiftungsrat und das April 2011, Magdeburg, Direktorium in wissenschaftlichen und technischen Fragen. MinRat Friedel Cramer, BMELV, Bonn, (seit Oktober 2011), Er ist verantwortlich für die Bewertung der wissenschaftlich- Prof. Dr. Wulf Diepenbrock, Martin-Luther-Universität Halle- technischen Arbeiten und fördert die Verbindung mit Einrich- Wittenberg, (bis Februar 2011), tungen des In- und Auslandes. Prof. Dr. Birgit Dräger, Martin-Luther-Universität Halle-Wit- tenberg, (seit März 2011), Mitglieder des Wissenschaftlichen Beirates Prof. Dr. Lothar Willmitzer, Max-Planck-Institut für Molekulare Pflanzenphysiologie Potsdam-Golm, Prof. Dr. Christian Jung, Kiel, (Vorsitz), Prof. Dr. Christian Jung, Christian-Albrechts-Universität zu Prof. Dr. Ralph Bock, Potsdam-Golm, (stellv. Vorsitz), Kiel, (Vorsitz Wissenschaftlicher Beirat) Prof. Dr. George Coupland, Köln, Prof. Dr. Ralph Bock, Max-Planck-Institut für Molekulare Pflan- Prof. Dr. Thomas Dandekar, Würzburg, zenphysiologie Potsdam-Golm, (stellv. Vorsitz Wissenschaftli- PD Dr. Christiane Gebhardt, Köln, (Vorsitz Genbank-Beirat), cher Beirat). Prof. Dr. Marcus Koch, Heidelberg, a.o. Univ. Prof. Dr. Josef Loidl, Wien, Prof. Dr. Jerzy Paszkowski, Genf, Das Direktorium ist ein Kollegialorgan, zusammengesetzt Prof. Dr. Dierk Scheel, Halle, aus den Leitern der wissenschaftlichen Abteilungen und dem Prof. Dr. Dietmar Schomburg, Braunschweig, (seit November Administrativen Leiter. Der Stiftungsrat bestellt einen der wis- 2011), senschaftlichen Abteilungsleiter für drei Jahre zum Geschäfts- Dr. Ralf-Michael Schmidt, Ludwigshafen, (bis Oktober 2011), führenden Direktor. Dieser bildet gemeinsam mit dem Admi- Prof. Dr. Chris-Carolin Schön, München. nistrativen Leiter die Geschäftsführung, die die Stiftung nach Maßgabe der Geschäftsordnung gerichtlich und außergericht- lich vertritt.

283 Gremien und Mitarbeiter/-innen in speziellen Funktionen/Boards of the IPK and Employees with Special Responsibilities

Aufgrund der Sonderstellung der Genbank im Servicebereich Mitarbeiter/-innen des IPK in speziellen Funktionen und um den mit der globalen Erhaltung genetischer Ressour- cen verbundenen Fragestellungen gesondert Rechnung zu Dr. Ulrike Lohwasser (Qualitätsmanagement-Beauftragte), tragen, verfügt die Genbank satzungsgemäß über einen Gen- Thomas Lüttge (Qualitäts-Beauftragter für die Abteilung VZD), bank-Beirat, der dem Wissenschaftlichen Beirat beratend zur Dr. Winfriede Weschke und Dr. Jochen Kumlehn (Beauftrag- Seite steht. te für Biologische Sicherheit), Dr. Hans-Peter Mock (Beauftragter für Betäubungsmittel und Mitglieder des Genbank-Beirates Gefahrstoffe), Prof. Dr. Andreas Graner (Beauftragter für Strahlenschutz), PD Dr. Christiane Gebhardt, Köln, (Vorsitz) Dr. Helmut Bäumlein (Ombudsmann), (bis November 2011), Dr. Tankred Schuhmann (Beauftragter für Datenschutz), Dr. Theo J. L. van Hintum, Wageningen, (stellv. Vorsitz) (bis No- Ellen Weiß (Gleichstellungsbeauftragte), vember 2011), Hans-Jürgen Winkelmann (Fachkraft für Arbeitssicherheit), Prof. Dr. Heiko Becker, Göttingen, Steffen König (Schwerbehindertenbeauftragter), Dir. und Prof. Dr. Frank Ordon, Quedlinburg, Peter Schreiber (Beauftragter für Havarie- und Katastrophen- Dr. habil. Heiko Kurt Parzies, Stuttgart-Hohenheim, schutz), († August 2011), Carmen Höpfner (Beauftragte für Lehrausbildung). Dr. habil. Hans Günter Welz, Wolfenbüttel.

Betreuer des IPK-Doktorandenprogramms Mitglieder des IPK-Personalrates Dr. Timothy F. Sharbel Thomas Kruse (Vorsitzender), Dr. Udo Conrad Sibylle Pistrick (1. Stellvertreterin), Dr. Andreas Houben Frank Schröder (2. Stellvertreter), Dr. Michael F. Mette Kathrin Gramel-Eikenroth, Steffen König, Melanie Ruff, Mitglieder des PhD Student Board Birgit Schäfer, Nicole Wahle, Nicole Schmid (Sprecherin) Evelin Willner, Teilsammlungen Nord, Malchow/Poel, Stefan Heckmann Dagmar Böhmert (Ersatzmitglied), Katja Herrmann Ute Riedel (Ersatzmitglied). Lydia Gerson Steffie Witter Mathias Franke Tiina Liiving Nadine Bernhardt Jonathan Brassac Andreas Finke Manuela Nagel

284 Leibniz Institute of Plant Genetics and Governing Board Chair: Crop Plant Research (IPK) MinDirig Dr. Joachim Welz Vice Chair: Corrensstraße 3 RD Peter Hassenbach D-06466 Gatersleben Scientific Advisory Board Germany Chair: Phone: +49 (0)39482 50 Board of Directors Prof. Christian Jung Fax: +49 (0)39482 5500 Prof. Andreas Graner 1) Email: [email protected] Managing Director Internet: http://www.ipk-gatersleben.de Genebank Advisory Board Sybille-Andrea Lorenz 1) Chair: As of 1 January 2010 Administrative Director PD Dr. Christiane Gebhardt Managing Office Prof. Thomas Altmann Roland Schnee Prof. Ingo Schubert Personnel Council Prof. Nicolaus von Wirén Chair: Thomas Kruse

Programmes

Groups

Plant Genome Resources Centre (PGRC) Coordinator: Dr. Patrick Schweizer

Bioinformatics Coordinator: Prof. Falk Schreiber 1) Management

Plant Genome Resources Centre (PGRC) Coordinator: Dr. Patrick Schweizer

Bioinformatics Coordinator: Prof. Falk Schreiber 1) Management

Plant Genome Resources Centre (PGRC) Coordinator: Dr. Patrick Schweizer

Bioinformatics Coordinator: Prof. Falk Schreiber 1) Management

Leibniz-inStitut FüR PFLanzengenetik und kuLtuRPFLanzenFoRSchung (iPk) 2010/2011 Scientific Report Forschungsbericht/