Revision: 0 Effective Date: 12/11/2018 at incubated blood Response: Cultural Expected 5% With Appearance: Prepared Appearance: Dehydrated Specifications Control Quality 2. 1. Procedure Test 6. 5. 4. 3. 2. 1. Preparation toSDS Refer Precaution specifications. performance to meet required as supplemented and/or adjusted be may Formula 25°C at ±0.2 pH: 7.4 Agar Sodium Extract Yeast Soy Tryptose Typical Formulation eg: mixtures appropriate antibiotic of addition the by groups various for selective be made of recovery givesgood the medium ‘chocolated’ is the blood When appearances, gives colonial medium growing of is capable blood, of with addition which, the base agar A veryrich Description in humans. . 2 No. Base Agar Blood Use Intended Sheet Specification Technical

accordance with established laboratory procedures. procedures. laboratory established with accordance orunder anaerobically, aerobically, plates Incubate oxygen each Process pouring. wellbefore Mix A 45 toCool minutes. 15 for 121°C at Autoclave medium. the dissolve completely to minute one boil for and agitation frequent with Heat 3 Suspend Peptone

septically add 5 add septically Staphylococc C. perfringens Gardnerella Str C

sheep or sheep

hloride eptococc

-

stable and oxygen and stable

-

9.5 50°C.

Blood Agar Base is not intended for use in the diagnosis of disease or other conditions conditions or other disease of diagnosis in the use for intended is not Base Blood Agar

sample horse horse grams of the medium in one liter of purified water. purified inof one liter the medium of grams appropriate atmosphere and temperature and examined for growth after incubation. after growth for examined and temperature and atmosphere appropriate us spp. us spp. spp.

-

us 7% sterile, defibrinated horse or sheep blood or sheep horse defibrinated 7% sterile, – is used with blood for the and cultivation of a wide variety of fastidious fastidious of variety wide a of cultivation and isolation the for with blood used is /

12 15 S blood, medium is red and opaque. is red and blood, medium light is withoutblood medium Prepared Neomycin (X Neomycin – 5 5 g/L 2.5 treptococc as appropriat as

.0 .0 .0 .0 BloodAgar Base ( No.2 Colistin/Oxolinic acid (X acid Colistin/Oxolinic – Powder is homogeneous, free flowing, and beige. beige. and flowing, free homogeneous, is Powder

Colistin/Oxolinic acid (X acid Colistin/Oxolinic g/L g/L g/L g/L - labile streptolysins. streptolysins. labile Cultural response on Blood Agar Based No. 2 with 5 with 2 No. Based Agar Blood on response Cultural

us 0

15) (X e spp.

and inoculate directly onto the surface of the medium. Streak Streak for the medium. of surface the onto directly inoculate and

– 0

Colistin/Naladixic acid (X Colistin/Naladixic 16)

patterns and pigment production of of production pigment and patterns

0 11)

0 13) conditions of increased CO increased of conditions

NCM0040 yellow beig

. Haemophilus Haemophilus

0 12) e

, and trace to slightly hazy. hazy. to slightly trace and , fastidious organisms fastidious )

spp. The medium can medium The spp. 2 indicative

- (5 7 % -

10%) in 10%) sheep or sheep

value. value.

. The horse horse

Revision: 0 Effective Date: 12/11/2018 3. 2. 1. References closed. tightly container light keeping by storage tempera at same the environment humidity low in a the container 2 at media culture dehydrated Store Storage 3. 2. 1. Procedure the of Limitations directed. as when stored container in its intact to medium applies Expiry the originalcolor. from orchanged has appearance or if flowing, free dehy The on the container. stamped date toexpiration Refer Expiration 4. 3. 2. 1. as: described media agar blood on hemolysis of types 18 after reactions hemolytic growthand for medium Examine Results used thatfor beshould minimum the listed are organisms The Sheet Specification Technical pneumoniae pneumoniae Streptococcus coli Campylobacter jejuni Campylobacter jejuni Campylobacter aeruginosa Pseudomonas aureus coli Escherichia faecalis

examination of food, food, of examination Splittst F. D. and C., Vanderzant, manualBAM/default.htm www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalytical The 9. No. Monograph NY streptococci. of study Research. the Medical for Institute Rockefeller for agar blood of use The 1919. H. J. Brown, procedures. laboratory established increas under media base agar blood incubate performance, beta of reactions hemolytic influence can atmosphere Incubation alpha and are Such strains blood. in animal differences by be affected to shown been have D streptococci group some strainsof of reactions Hemolytic medium. this on grow to or fail poorly grow that be encountered strainsmay some variation, nutritional Due to lysis. Alpha thein medium. no change No des hemolysis. no indicates hemolysis (γ) Gamma colony. the zone surrounding clear a producing red blood cells, of lysis is the (β) hemolysis Beta the medium. of discoloration green a produces colony. This methem to hemoglobin of reduction is the (α) hemolysis Alpha

- prime hemolysis (α’) is a small zone of complete hemolysis surrounded by an area of partial of area an by surrounded hemolysis complete zoneof is a small hemolysis (α’) prime

- hemolytic on sheep blood agar. blood sheep on hemolytic ATCC ATCC® 43478 ATCC®

ATCC® 33291 ATCC® 29428 ATCC® ® 25922 ATCC® 29212 ATCC® 4 ATCC® 25923 ATCC® th ATCC® 19615 ATCC® ed., p. 1113. American Public Health Association, Washington, D.C. Association, Health Washington, Public American p. 1113. ed., 27853 ATCC® .

ATCC® 6305 ATCC®

– oesser (eds). (eds). oesser

30°C away from direct sunlight. Once opened and recapped, place place recapped, and opened Once direct sunlight. from away 30°C

Inoculum (CFU) Inoculum

beta 4Q streak 4Q streak 4Q streak 4Q streak 4Q 4Q Approx. Approx. 50 50 50 50 2015

streak - - - - -

hemolytic on horse, human, and rabbit blood agar blood rabbit and human, horse, on hemolytic 200 200 200 200 . Compendium of methods for the microbiological microbiological the for methods of Compendium . truction of red blood cells occurs and there is and there occurs cells red blood of truction

drated medium should be discarded if not not if discarded be should medium drated -

quality control testing. quality control 24 and 48 hours incubation. There are four are four There incubation. hours 48and 24

ed CO

oglobin in the medium surrounding the oglobin medium the in Good growth Good growth Good growth Good growth Good growth - hemolytic streptococci. For optimal optimal For streptococci. hemolytic Growth 2 ≥70% ≥70% ≥70% ≥

70% (5 (5 ture. Protect from and moisture from ture. Protect - Expected Results Expected

10%), in accordance with accordance in 10%),

Weak βhemolysis Weak α hemolysis hemolysis β hemolysis β hemolysis β Reactions NA NA NA NA

Revision: 0 Effective Date: 12/11/2018 6. 5. 4. Sheet Specification Technical

irbooy rcdrs adok vl 1 . 1.61 p. 1 vol. D.C. Washington, handbook, procedures microbiology Isenberg, H. D. (ed.). 1992. Interpretation of aerobic bacterial growth on primary culture media, Clinical D. C. Washington, 6 microbiology, clinical of Manual (eds.). Yolken H. R. and Ruoff, K. L. 1995. wastewater, water and of (eds.). Eaton D. A. and Clesceri, S. L. E., A. Greenberg,

Streptococcus

23 rd

ed. American Public Health Association, Washington, D.C. Association, Washington, Health Public American ed. , p. 299 - 305. 305. In

P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, - 1.67. American Society for Microbiology, Microbiology, for Society American 1.67. 2017 t h

. Standard methods for the examinati the for methods Standard . ed. American Society for Microbiology, Microbiology, for Society American ed.

on