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Project FISH-DIVA : Maggot

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Project FISH-DIVA : Maggot Project FISH-DIVA Maggot – Bioconversion Research Program in Indonesia Concept of New Food Resources Results and Applications 2005 - 2011 Project FISH-DIVA Maggot – Bioconversion Research Program in Indonesia Concept of New Food Resources Final Report Results and Applications Saurin HEM 2005 - 2011 November 2011 K E N M Final Report A E N N A T E IK R ER IA P N K N Saurin Hem ELAUTAN DA Centre for Aquaculture Research and Development November 2011 Research Institute for Ornamental Fish Project FISH-DIVA Maggot – Bioconversion Research Program in Indonesia Concept of New Food Resources Results and Applications 2005-2011 Final Report Saurin HEM November 2011 Contents SUMMARY ......................................................................................................................................... 1 Acknowledgment ................................................................................................................................ 2 General reviews ................................................................................................................................. 3 Current and new rend needs in the Future Aquaculture ................................................................3 Fishmeal replacement (resources constrains) ...............................................................................3 Historic context .................................................................................................................................. 3 Understanding the aquaculture situation and the problems faced .....................................................5 Basic principles of my Mission in Indonesia ....................................................................................... 6 IRD Mission related to Bioconversion Program - Terms of Reference (TOR) and duty. ................ 6 Synergic Collaboration between IRD/CRIA/DGA teams and actions programs - Partnership and Collaboration based on a mutual goal of interest. ..........................................................................6 Reinforce the future capacity building, ........................................................................................... 7 Back-ground Understanding .............................................................................................................. 8 Principle of Bioconversion as a natural processes .........................................................................8 Palm Kernel Meal ........................................................................................................................... 8 Bioconversion program in Indonesia .................................................................................................. 9 First contact with BSF .................................................................................................................... 9 An Open Gate ................................................................................................................................ 9 Identification confirmed at LIPI insectarium Cibinon Bogor. ...........................................................9 Vulgarizations Maggot program beside the population opinion ....................................................10 Evidence research needs for Confirmation ..................................................................................10 Insect Hermetia illucens (Linnaeus, 1758) .......................................................................................11 Classification and Characteristics .................................................................................................... 11 Repartition and distribution zone of presence of insect Hermetia illucens .......................................12 Biology learned from literature ......................................................................................................... 12 A word on the anatomy and morphology aspect ..............................................................................13 Anatomy ........................................................................................................................................... 13 The mouth part of the adult insect, ............................................................................................... 13 Anatomy of the sexual organs ...................................................................................................... 14 Research Approach to understand the pre-pupa stage ...................................................................15 Eggs collection ............................................................................................................................. 15 Tentative to establish the Larvae Aging ....................................................................................... 15 First tentative of intensive culture of insect Hermetia in purpose of mass production of eggs ......... 19 Intensive culture of insect Hermetia in enclosed environment .....................................................19 Bioconversion as an intensification ecological an example ..........................................................20 Research on biofertilizer a by-product from bioconversion process ............................................. 20 Research on microbiology ............................................................................................................... 21 Yeast and fungus ............................................................................................................... 21 Bacteria ............................................................................................................................. 22 Application of Bioconversion program to Develop the rural aquaculture – Singkut Village Sarolangun Jambi (SUMATRA) ....................................................................................................... 23 Introduction ................................................................................................................................... 23 Bioconversion in “Pilot program”: Maggot as an alternative fishmeal replacement ...................... 23 PKM supply issue ......................................................................................................................... 24 Conclusion .................................................................................................................................... 25 “Minilarvae”a second aspect of application of Bioconversion Research .......................................... 27 Bioconversion of organic wastes other than PKM (Palm Kernel Meal), in Indonesia ..................... 29 Back Grounds and research scope ..............................................................................................29 Intensive of the insect Hermetia illucens rearing to obtain the mass production of eggs ............. 29 Getting to know the life cycle during the larval stage.........................................................29 Care with precautions during the development of pupae stage ........................................30 Precaution and care of adult insects .................................................................................30 Results of productions: ......................................................................................................30 Bioconversion of organic wastes other than the PKM ..................................................................31 Waste from fish market: ............................................................................................................... 31 Ensiling process of fermentation lactic acid .................................................................................32 Analyses of larvae enriched ......................................................................................................... 32 Using enriched larvae for feeding Red Gurami Padang ..................................................................34 Biodegradation of the by-product from Tahu (Ampas tahu) and the rest of Copra ....................... 36 Conclusions recommendations: ....................................................................................................... 38 An unexpected Recognition ............................................................................................................. 39 Bibliography - References used ....................................................................................................... 40 Annexes ........................................................................................................................................... 43 Final Report Saurin HEM , Bioconversion Indonesia 2005 – 2011 1 SUMMARY The bioconversion of Palm Kernel Meal (PKM) is a natural process consisting of extracting nutrients residue in this by-product, into a form of insect larvae biomass. This is a new source of animal proteins and fats for aquaculture. The insect in the bioconversion process (Hermetia illucens, F. Stratiomyidae, O. Diptera) or Black Soldier Fly (BSF) is well known in USA as amongst the best biodegradation agents. The first research program in 2005 to 2007 consisted of investigating the biology and life-cycle of Hermetia in the Indonesian climatic conditions. Many data have existed especially from USA, where BSF has considered as benefited insect which may provide many good services for humanity and environment. However, previous research before any vulgarization to the population
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