mSECTM1B VersaClone cDNA Catalog Number: RDC0740
Specifications: Description This shuttle vector contains the complete ORF for the gene of interest, Gene: mSECTM1B along with a Kozak consensus sequence for optimal translation initiation. It is inserted NotI to AscI. The gene insert is flanked with Accession: NP_081183 convenient multiple cloning sites which can be used to easily cut and transfer the gene cassette into your desired expression vector. Insert size: 652bp Preparation and Storage Concentration: 10µg at 0.2μg/μL Formulation cDNA is provided in 10 mM Tris-Cl, pH 8.5
Shipping Ships at ambient temperature
Stability 1 year from date of receipt when stored at -20°C to -80°C Storage Use a manual defrost freezer and avoid repeated mSECTM1B cDNA freeze-thaw cycles.
Plasmid
Sectm1b secreted and BstAPI NdeI transmembrane 1B [ Mus BbeI musculus (house mouse) ] KasI SspI NarI SfoI BmgBI HpaI HindIII XmnI Also known as: K12; Sectm1 ApaI BamHI BanII EcoRV PspOMI BmtI NheI Summary: NotI SECTM1 is a single-pass type I EagI transmembrane protein. SECTM1 BstEII and CD7 are cognate proteins that AMP BclI are located next to each other on Bpu10I RDC0740mSECTM1B (1-212) human chromosome 17. Mouse SECTM1B protein binds to mouse NmeAIII 3386 bps BbsI BsrFI PpuMI CD7 and may be involved in PstI AscI BtgZI AhdI BssHII thymocyte signaling. SECTM1 Bst1107I XbaI costimulates CD4 and CD8 T cell SalI proliferation and induces IFN SmaI XmaI gamma production. ApoI EcoRI COLE1 PspXI XhoI SapI Eco47III AlwNI PciI
FOR RESEARCH USE ONLY
NOT FOR USE IN HUMANS
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> RDC0740 Plasmid DNA Sequence 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 101 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 201 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 401 ggagacgtgt taacaagctt ggatccgata tcgctagcgc ggccgccacc atgctggcct actctgtaac atcctctggc ctgtttccca gaatgctctg 501 ggccctcctt ctactggcag cctccctgaa tgcctataac catgtctggg acaaaccttg ttgcactgag catgaagtat ctgtaaacag aggcagccgc 601 gtggtgatgg cctgtaatat ctccaacaat ctcagagacg tcaccattga gttggttacc agtaaaaaga ctagcatcat cttcaatcaa acgcctccag 701 gaaactactc taaggattca tggcagcttc atattcaagg aggccaggcc cagctggtga tcacagatgc tcagggcaaa cactcagggg agtactggtg 801 gaagctgcgt ggattccagg cagagttcaa aaacttcaac ctgattgtta atgccgcaga cagacagaag acagaggact tgccagtcac taaggtccct 901 gataagcccc caactgcagt gcggacagaa gttatcatca tcatcgccat tgctaccacc atcatcatca caggaatcgg tgtattcgtt tggtacaagc 1001 aattccctgt agctccacag atacaaatgt cagtaccttg tctgattcat gggtctcctg gtatccccta cctgacactg cccccctaaa ggcgcgccag 1101 tatactctag agtcgacacc cggggaattc ctcgagcgct cgtctctagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 1201 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 1301 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 1401 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 1501 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 1601 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 1701 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 1801 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1901 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 2001 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 2101 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 2201 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 2301 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 2401 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 2501 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 2601 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 2701 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 2801 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 2901 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 3001 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 3101 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 3201 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 3301 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
> RDC0740 Translated Insert Sequence 1 mlaysvtssg lfprmlwall llaaslnayn hvwdkpccte hevsvnrgsr vvmacnisnn lrdvtielvt skktsiifnq tppgnyskds wqlhiqggqa 101 qlvitdaqgk hsgeywwklr gfqaefknfn livnaadrqk tedlpvtkvp dkpptavrte viiiiaiatt iiitgigvfv wykqfpvapq iqmsvpclih 201 gspgipyltl pp
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