Completing genome-scale metabolic networks 1
S7 Predicted ergosterol pathway
trans,trans-farnesyl diphosphate biosynthesis
2 trans, trans-farnesyl diphosphate
diphosphate Farnesyl-diphosphate farnesyltransferase.: 99438 2.5.1.21
presqualene diphosphate NAD(P)H Farnesyl-diphosphate farnesyltransferase.: 99438 2.5.1.21 diphosphate NAD(P)+
a reduced acceptor 1.14.99.7 O2
an acceptor H2O
(S)-2,3-Epoxysqualene
Lanosterol synthase.: NOP10 Lanosterol synthase.: Chlre2_kg.scaffold_8000029 Lanosterol synthase.: HIRA3401 5.4.99.7
lanosterol
O2 NADPH sterol 14 desaturase: CYP51G1 1.14.13.70 H2O NADP+
4,4-dimethyl-14α-hydroxymethyl-5α-cholesta-8,24-dien-3β-ol
O2 sterol 14 desaturase: CYP51G1 NADPH 1.14.13.70 H+ NADPH O 2 sterol 14 desaturase: CYP51G1 2 H2O + 1.14.13.70 NADP formate 4,4-dimethyl-14α-formyl-5α-cholesta-8,24-dien-3β-ol NADP+ O2 NADPH sterol 14 desaturase: CYP51G1 1.14.13.70
formate H2O NADP+
4,4-dimethyl-5-α-cholesta-8,14,24-trien-3-β-ol
NADPH sterol redcutase: ERG4/ERG24 1.3.1.70
NADP+
4,4-dimethylzymosterol
O2 NAD(P)H sterol desaturase: ERG25 1.14.13.72 H2O NAD(P)+
4α-hydroxymethyl-4β-methyl-5α-cholesta-8,24-dien-3β-ol
O2 sterol desaturase: ERG25 NAD(P)H 1.14.13.72
O2 2 H2O NAD(P)+
4α-formyl-4β-methyl-5α-cholesta-8,24-dien-3β-ol
O2 sterol desaturase: ERG25 NAD(P)H 1.14.13.72
H2O NAD(P)+
sterol desaturase: ERG25 1.14.13.72 4α-formyl-5α-cholesta-8,24-dien-3β-ol 4α-carboxy-4β-methyl-5α-cholesta-8,24-dien-3β-ol α α β O2 sterol desaturase: ERG25 CO2 4 -hydroxymethyl-5 -cholesta-8,24-dien-3 -ol C-3 sterol dehydrogenase: BHSD1 NAD(P)H 1.14.13.72 O2 2 H2O NAD(P)H NAD(P)+
4α-methyl-5α-cholesta-8,24-dien-3-one H2O H2O NAD(P)+ NAD(P)+ sterol desaturase: ERG25 NADPH 1.1.1.270 1.14.13.72 4α-carboxy-5α-cholesta-8,24-dien-3β-ol O + 2 NAD(P) NAD(P)H steroid dehydrogenase: BHSD1 + NADP (MapMan): gwH.42.21.1 CO C-3 sterol dehydrogenase/ 2 C-4 decarboxylase: ERG26 NAD(P)H 1.1.1.170
4-α-methylzymosterol 5α-cholesta-8,24-dien-3-one
O NADPH 2 1.1.1.270
+ CO2 NADP
S-adenosyl-L-methionine sterol methyltransferase: SMT1 2.1.1.41
S-adenosyl-L-homocysteine
episterol
NADPH C-5 sterol desaturase: SC5D O2
NADP+ 2 H2O
5,7,24(28)-ergostatrienol
NADPH C-22 desaturase: CYP710B1 O2
NADP+ 2 H2O
5,7,22,24(28)-ergostatetraenol
H+ C-24 sterol reductase: ERG4/ERG24 NADPH 1.3.1.71
NADP+
ergosterol
Figure 1: The predicted ergosterol pathway. Shown are reaction together with the annotated enzymes as given by the ChlamyCyc database 1. The pathway was visualized using the Pathway Tools software 2 Completing genome-scale metabolic networks 2
References
[1] P. May, J.-O. Christian, S. Kempa and D. Walther, BMC Genomics, 2009, 10, 209.
[2] P. D. Karp, S. Paley and P. Romero, Bioinformatics, 2002, 18 Suppl 1, S225–S232.