DOCSLIB.ORG

Rhizobium Indigoferae Sp. Nov. and Sinorhizobium Kummerowiae Sp

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Rhizobium Indigoferae Sp. Nov. and Sinorhizobium Kummerowiae Sp International Journal of Systematic and Evolutionary Microbiology (2002), 52, 2231–2239 DOI: 10.1099/ijs.0.02030-0 Rhizobium indigoferae sp. nov. and Sinorhizobium kummerowiae sp. nov., respectively isolated from Indigofera spp. and Kummerowia stipulacea 1 College of Biological Ge Hong Wei,1,2 En Tao Wang,3 Zhi Yuan Tan,1 Ming E Zhu2 Sciences, China 1 Agricultural University, and Wen Xin Chen Beijing 100094, People’s Republic of China 2 Author for correspondence: Wen Xin Chen. Tel: j86 10 62891854. Fax: j86 10 62891055. Northwest Science and e-mail: wenxinIchen!263.net Technology University of Agriculture and Forestry, Yangling, Shaanxi 712100, People’s Republic of China Forty-eight rhizobial isolates from root nodules of Indigofera and Kummerowia, two genera of annual or perennial wild legumes growing in the 3 Departamento de Microbiologı!a, Escuela Loess Plateau in north-western China, were characterized by a polyphasic Nacional de Ciencias approach. Two main groups, cluster 1 and cluster 2, were defined based upon Biolo! gicas, Instituto the results of numerical taxonomy, SDS-PAGE of whole-cell proteins and DNA Polite! cnico Nacional, Prol. de Carpio y Plan de Ayala relatedness. All the isolates within cluster 1 were isolated from Indigofera and s/n, 11340, Me! xico DF, they were identified as Rhizobium strains by 16S rRNA gene analysis. DNA Mexico relatedness of 295–489% was obtained among the cluster 1 isolates and the reference strains for defined Rhizobium species. Cluster 2 consisted of isolates from Kummerowia stipulacea and was identified as belonging to Sinorhizobium by 16S rRNA gene analyses. DNA relatedness varied from 52to 417% among the isolates of cluster 2 and reference strains for Sinorhizobium species. Considering the existence of distinctive features among these two groups and related species within the genera Rhizobium and Sinorhizobium, we propose two novel species, Rhizobium indigoferae sp. nov. for cluster 1, with isolate CCBAU 71714T (l AS 1.3046T) as the type strain, and Sinorhizobium kummerowiae sp. nov. for cluster 2, with isolate CCBAU 71042T (l AS 1.3045T) as the type strain. Keywords: Rhizobium indigoferae, Sinorhizobium kummerowiae, diversity, phylogeny INTRODUCTION continual investigation of rhizobial resources. At the time of writing, 31species have been described within Rhizobia are useful bacterial resources because of their six genera: Allorhizobium (de Lajudie et al., 1998b), symbiotic nitrogen-fixing ability. They form nodules Azorhizobium (Dreyfus et al., 1988), Bradyrhizobium on roots, and occasionally on stems, of many leg- (Jordan, 1984; Kuykendall et al., 1992; Xu et al., uminous plants and reduce N# to ammonia, which can 1995), Mesorhizobium (Jarvis et al., 1982, 1997; Chen be used by the host plants. In the last two decades, the et al., 1991, 1997; Nour et al., 1994, 1995; de Lajudie classification of rhizobia has developed rapidly, mostly et al., 1998b; Wang et al., 1999), Rhizobium (Amarger by the application of polyphasic taxonomy and by the et al., 1997; van Berkum et al., 1998; Chen et al., 1997; Jordan, 1984; Lindstro$ m, 1989; Martı!nez-Romero et ................................................................................................................................................. al., 1991; Segovia et al., 1993; Wang et al., 1998) and Published online ahead of print on 12 July 2002 as DOI 10.1099/ Sinorhizobium (Chen et al., 1988; de Lajudie et al., ijs.0.02030-0. 1994; Rome et al., 1996). Azorhizobium and Bradyrhi- Full details of the GjC contents and DNA–DNA relatedness of the zobium are phylogenetically divergent from the other individual novel isolates are available as supplementary material in IJSEM Online (http://ijs.sgmjournals.org/). rhizobia. The remaining genera, Allorhizobium, Meso- The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene rhizobium, Rhizobium and Sinorhizobium, are closely sequences of strains CCBAU 71042T and CCBAU 71714T are AF364068 and related to each other and they are intermingled with AF364067. plant-pathogenic bacteria belonging to the genus 02030 # 2002 IUMS Printed in Great Britain 2231 G. H. Wei and others Agrobacterium and non-symbiotic bacteria within the order to characterize the isolates and strains. The simple genus Ensifer (Casida, 1982). A novel group of root- matching coefficient Ssm and UPGMA (Sneath & Sokal, nodule bacteria was recently identified as belonging to 1973) were used for clustering analysis of phenotypic Methylobacterium (Sy et al., 2001). Although most of features. The mean similarity for each isolate within a cluster the defined root-nodule bacteria are members of the α- was estimated to present the phenotypic variation in the cluster, as described previously, and the isolate with the subclass of the Proteobacteria, novel taxa of root- highest mean similarity was chosen as the central isolate or nodule bacteria, Ralstonia taiwanensis (Chen et al., representative for the cluster (Sneath & Sokal, 1973). 2001) and Burkholderia sp. (Moulin et al., 2001), have Generation time of the isolates was determined spectro- been reported, both being members of the β-Proteo- photometrically (Yelton et al., 1983) in YM broth (Vincent, bacteria. 1970). Legumes in the genera Indigofera and Kummerowia are SDS-PAGE of whole-cell proteins. Methods described pre- annual or perennial wild plants. Most of them are viously (Tan et al., 1997) were used for soluble protein drought-tolerant and they are held in high esteem as extraction, SDS-PAGE and scanning of the protein patterns foliage, green manure crops or in preventing soil using an Extra-Scanner densitometer (LKB). Jaccard’s erosion (Allen & Allen, 1981). Kummerowia species are coefficient (Sj) and UPGMA (Sneath & Sokal, 1973) were used for cluster analysis. used as herbal medicine in China for treating diarrhoea and fever. These plants form root nodules but their PCR-based RFLP of 16S rRNA genes. Procedures described microsymbionts have not been characterized system- previously (Wang et al., 1998) were used for PCR amplifi- atically. The north-western Loess Plateau region of cation of complete 16S rRNA genes with the universal China, including the provinces of Shaanxi, Gansu and forward primer P1 and the universal reverse primer P6 (Tan et al., 1997), for digestion of the PCR products with the Autonomous Region of Ningxia, is located in a restriction endonucleases MspI, HinfI, HaeI and RsaI and temperate zone with a semi-arid climate and soils poor for separation of the digested fragments in 3n0% agarose in organic matter. During the summer of 1992, we gels. Primer P1 (5h-CGggatccAGAGTTTGATCCTGGTC- obtained some rhizobial isolates from different plants AGAACGCT-3h) corresponds to positions 8–37 and primer and different geographical areas in a survey of rhizo- P6 (5h-CGggatccTACGGCTACCTTGTTACGACTTCA- bial resources in these regions. Previously, we have CCCC-3h) corresponds to positions 1479–1506 in the Escher- characterized isolates from Amorpha fruticosa, Glycyr- ichia coli 16S rDNA (Willems & Collins, 1993; Yanagi & rhiza, Gueldenstaedtia and other genera according to Yamasato, 1993). Lower-case letters indicate the BamHI their geographical or host origin (Wang et al., 1999; restriction site. Tan et al., 1999). Considering the potential value of Amplification, cloning and sequencing of 16S rDNA. This Indigofera and Kummerowia in agriculture and re- study was performed only for representative isolates of the forestation and the unknown taxonomic position of novel clusters. Full-length 16S rRNA genes (1500 bp) were the rhizobia associated with them, we decided to amplified from isolates by PCR in a 100 µl reaction mixture characterize isolates from Indigofera and Kummerowia using primers P1 and P6. Amplified 16S rDNA was purified plants in these regions with a polyphasic approach. from 0n8% low-melting-point agarose gel by the method of Wieslander (1979). Purified rDNA and plasmid vector The aims of the research were to check the diversity pUC18 were restricted with BamHI and ligated at 14 mC for and to classify the isolates by both phenotypic and 16 h. Ligated plasmids were transformed into E. coli DH5α genetic methods. and transformants were selected using the blue\white screening procedure (Sambrook et al., 1989). Plasmids METHODS containing 16S rDNA were extracted and purified according to the methods of Tiesman & Rizzino (1991). Purified Isolates and strains. The isolates and reference strains used plasmids were sequenced using an ABI377 automatic se- are listed in Table 1. The 21 isolates from Indigofera spp. and quencer (Applied Biosystems). Two independent clones of 27 isolates from Kummerowia stipulacea were fast-growing PCR-amplified 16S rRNA genes from each isolate were rhizobia that formed single colonies with diameters of sequenced. 2–3 mm within 3 days on YMA. Rhizobia were isolated Sequence analysis and reconstruction of the phylogenetic from fresh nodules collected from plants growing in the field tree. The 16S rRNA gene sequences obtained and those of by the standard method on YMA medium (Vincent, 1970). related bacteria obtained from GenBank were aligned using Single colonies were picked and checked for purity by the program in the Wisconsin package (Genetics repeated streaking and by microscopic examination. Nodu- Computer Group, 1995). Sequence similarities and a phylo- lation with the original host plant of each isolate was genetic tree were generated and bootstrapped with 1000 checked in glass tubes, half-filled with vermiculite, as subsamples using version
Load more

Recommended publications
  • Revised Taxonomy of the Family Rhizobiaceae, and Phylogeny of Mesorhizobia Nodulating Glycyrrhiza Spp
    Division of Microbiology and Biotechnology Department of Food and Environmental Sciences University of Helsinki Finland Revised taxonomy of the family Rhizobiaceae, and phylogeny of mesorhizobia nodulating Glycyrrhiza spp. Seyed Abdollah Mousavi Academic Dissertation To be presented, with the permission of the Faculty of Agriculture and Forestry of the University of Helsinki, for public examination in lecture hall 3, Viikki building B, Latokartanonkaari 7, on the 20th of May 2016, at 12 o’clock noon. Helsinki 2016 Supervisor: Professor Kristina Lindström Department of Environmental Sciences University of Helsinki, Finland Pre-examiners: Professor Jaakko Hyvönen Department of Biosciences University of Helsinki, Finland Associate Professor Chang Fu Tian State Key Laboratory of Agrobiotechnology College of Biological Sciences China Agricultural University, China Opponent: Professor J. Peter W. Young Department of Biology University of York, England Cover photo by Kristina Lindström Dissertationes Schola Doctoralis Scientiae Circumiectalis, Alimentariae, Biologicae ISSN 2342-5423 (print) ISSN 2342-5431 (online) ISBN 978-951-51-2111-0 (paperback) ISBN 978-951-51-2112-7 (PDF) Electronic version available at http://ethesis.helsinki.fi/ Unigrafia Helsinki 2016 2 ABSTRACT Studies of the taxonomy of bacteria were initiated in the last quarter of the 19th century when bacteria were classified in six genera placed in four tribes based on their morphological appearance. Since then the taxonomy of bacteria has been revolutionized several times. At present, 30 phyla belong to the domain “Bacteria”, which includes over 9600 species. Unlike many eukaryotes, bacteria lack complex morphological characters and practically phylogenetically informative fossils. It is partly due to these reasons that bacterial taxonomy is complicated.
    [Show full text]
  • Enhanced Productivity of Serine Alkaline Protease by Bacillus Sp
    Malaysian Journal of Microbiology, Vol 6(2) 2010, pp. 133-139 http://dx.doi.org/10.21161/mjm.20109 Studies on nodulation, biochemical analysis and protein profiles of Rhizobium isolated from Indigofera species Kumari B. S., Ram M. R.* and Mallaiah K. V. Department of Microbiology, Acharya Nagarjuna University Nagarjuna Nagar-522 510, Guntur Dist, Andhra Pradesh, India. E-mail: [email protected] Received 3 July 2009; received in revised form 6 November 2009; accepted 20 November 2009 _______________________________________________________________________________________________ ABSTRACT Nodulation characteristics in five species of Indigofera viz., I .trita, I. linnaei, I. astragalina, I. parviflora and I. viscosa was studied at regular intervals on the plants raised in garden soil. Among the species studied, highest average number of nodules per plant of 23 with maximum sized nodules of 8.0 mm diameter was observed in I. astragalina. Biochemical analysis of root nodules of I. astragalina revealed that the leghaemoglobin content of nodules and nitrogen content of root, shoot, leaves and nodules were gradually increased up to 60 DAS, and then decreased with increase in age. Rhizobium isolates of five species of Indigofera were isolated and screened for enzymatic activities and total cellular protein profiles. All the five isolates showed nitrate reductase, citrase, tryptophanase and catalase activity while much variation was observed for enzymes like gelatinase, urease, caseinase, lipase, amylase, lysine decarboxylase and protease activities. Among the isolates studied, only the isolate from I. viscosa has the ability to solubilize the insoluble tricalcium phosphate. All the Rhizobium isolates exhibit similarity in protein content, except the isolate from I. viscosa which showed one additional protein band.
    [Show full text]
  • Ep 2434019 A1
    (19) & (11) EP 2 434 019 A1 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: (51) Int Cl.: 28.03.2012 Bulletin 2012/13 C12N 15/82 (2006.01) C07K 14/395 (2006.01) C12N 5/10 (2006.01) G01N 33/50 (2006.01) (2006.01) (2006.01) (21) Application number: 11160902.0 C07K 16/14 A01H 5/00 C07K 14/39 (2006.01) (22) Date of filing: 21.07.2004 (84) Designated Contracting States: • Kamlage, Beate AT BE BG CH CY CZ DE DK EE ES FI FR GB GR 12161, Berlin (DE) HU IE IT LI LU MC NL PL PT RO SE SI SK TR • Taman-Chardonnens, Agnes A. 1611, DS Bovenkarspel (NL) (30) Priority: 01.08.2003 EP 03016672 • Shirley, Amber 15.04.2004 PCT/US2004/011887 Durham, NC 27703 (US) • Wang, Xi-Qing (62) Document number(s) of the earlier application(s) in Chapel Hill, NC 27516 (US) accordance with Art. 76 EPC: • Sarria-Millan, Rodrigo 04741185.5 / 1 654 368 West Lafayette, IN 47906 (US) • McKersie, Bryan D (27) Previously filed application: Cary, NC 27519 (US) 21.07.2004 PCT/EP2004/008136 • Chen, Ruoying Duluth, GA 30096 (US) (71) Applicant: BASF Plant Science GmbH 67056 Ludwigshafen (DE) (74) Representative: Heistracher, Elisabeth BASF SE (72) Inventors: Global Intellectual Property • Plesch, Gunnar GVX - C 6 14482, Potsdam (DE) Carl-Bosch-Strasse 38 • Puzio, Piotr 67056 Ludwigshafen (DE) 9030, Mariakerke (Gent) (BE) • Blau, Astrid Remarks: 14532, Stahnsdorf (DE) This application was filed on 01-04-2011 as a • Looser, Ralf divisional application to the application mentioned 13158, Berlin (DE) under INID code 62.
    [Show full text]
  • 2010.-Hungria-MLI.Pdf
    Mohammad Saghir Khan l Almas Zaidi Javed Musarrat Editors Microbes for Legume Improvement SpringerWienNewYork Editors Dr. Mohammad Saghir Khan Dr. Almas Zaidi Aligarh Muslim University Aligarh Muslim University Fac. Agricultural Sciences Fac. Agricultural Sciences Dept. Agricultural Microbiology Dept. Agricultural Microbiology 202002 Aligarh 202002 Aligarh India India [email protected] [email protected] Prof. Dr. Javed Musarrat Aligarh Muslim University Fac. Agricultural Sciences Dept. Agricultural Microbiology 202002 Aligarh India [email protected] This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically those of translation, reprinting, re-use of illustrations, broadcasting, reproduction by photocopying machines or similar means, and storage in data banks. Product Liability: The publisher can give no guarantee for all the information contained in this book. The use of registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. # 2010 Springer-Verlag/Wien Printed in Germany SpringerWienNewYork is a part of Springer Science+Business Media springer.at Typesetting: SPI, Pondicherry, India Printed on acid-free and chlorine-free bleached paper SPIN: 12711161 With 23 (partly coloured) Figures Library of Congress Control Number: 2010931546 ISBN 978-3-211-99752-9 e-ISBN 978-3-211-99753-6 DOI 10.1007/978-3-211-99753-6 SpringerWienNewYork Preface The farmer folks around the world are facing acute problems in providing plants with required nutrients due to inadequate supply of raw materials, poor storage quality, indiscriminate uses and unaffordable hike in the costs of synthetic chemical fertilizers.
    [Show full text]
  • Phenotypic and Genotypic Characteristics of Rhizobia Isolated from Meknes-Tafilalet Soils and Study of Their Ability to Nodulate Bituminaria Bituminosa
    ... British Microbiology Research Journal 4(4): 405-417, 2014 SCIENCEDOMAIN international www.sciencedomain.org Phenotypic and Genotypic Characteristics of Rhizobia Isolated from Meknes-tafilalet Soils and Study of Their Ability to Nodulate Bituminaria bituminosa Btissam Ben Messaoud1, Imane Aboumerieme1, Laila Nassiri1, Elmostafa El Fahime2 and Jamal Ibijbijen1* 1Soil and Environment Microbiology Unit, Faculty of Sciences, Moulay Ismail University, Meknes, Morocco. 2Technical Support Unit for Scientific Research, CNRST in Rabat, Morocco. Authors’ contributions This work is a part of the PhD of the first author and was carried out in collaboration between all authors. Author BBM designed the study, authors BBM and IA performed the experiment and statistical analysis, wrote the protocol, and wrote the first draft of the manuscript. Authors JI and LN supervised the study and managed the literature searches, author EEF did the genotypic analysis. All authors read and approved the final manuscript. Received 28th July 2013 nd Original Research Article Accepted 22 November 2013 Published 12th January 2014 ABSTRACT Aims: The objectives were to isolate and characterize phenotypically and genotypically the rhizobial strains from the soils belonging to the Meknes-Tafilalet region in order to select strains that are able to nodulate Bituminaria bituminosa. Study Design: An experimental study. Place and Duration of Study: Department of biology (Soil & Environment Microbiology Unit) Faculty of Sciences, Moulay Ismail University and Technical Support Unit for Scientific Research, CNRST in Rabat; between January and August 2010. Methodology: Samples from 23 different sites belonging to the Meknes-Tafilalet region were collected in order to select rhizobial strains that are able to nodulate Bituminaria bituminosa.
    [Show full text]
  • Analysis of Rhizobial Strains Nodulating Phaseolus Vulgaris From
    Systematic and Applied Microbiology 37 (2014) 149–156 Contents lists available at ScienceDirect Systematic and Applied Microbiology j ournal homepage: www.elsevier.de/syapm Analysis of rhizobial strains nodulating Phaseolus vulgaris from Hispaniola Island, a geographic bridge between Meso and South America and the first historical link with Europe a b,c d César-Antonio Díaz-Alcántara , Martha-Helena Ramírez-Bahena , Daniel Mulas , e b b,c e,∗ Paula García-Fraile , Alicia Gómez-Moriano , Alvaro Peix , Encarna Velázquez , d Fernando González-Andrés a Facultad de Ciencias Agronómicas y Veterinarias, Universidad Autónoma de Santo Domingo, Dominican Republic b Instituto de Recursos Naturales y Agrobiología, IRNASA (CSIC), Salamanca, Spain c Unidad Asociada Grupo de Interacción Planta-Microorganismo, Universidad de Salamanca-IRNASA (CSIC), Spain d Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad, Universidad de León, Spain e Departamento de Microbiología y Genética, Universidad de Salamanca, Spain a r t i c l e i n f o a b s t r a c t Article history: Hispaniola Island was the first stopover in the travels of Columbus between America and Spain, and Received 13 July 2013 played a crucial role in the exchange of Phaseolus vulgaris seeds and their endosymbionts. The analysis of Received in revised form recA and atpD genes from strains nodulating this legume in coastal and inner regions of Hispaniola Island 15 September 2013 showed that they were almost identical to those of the American strains CIAT 652, Ch24-10 and CNPAF512, Accepted 18 September 2013 which were initially named as Rhizobium etli and have been recently reclassified into Rhizobium phaseoli after the analysis of their genomes.
    [Show full text]
  • Studies on Exopolysaccharide and Indole Acetic Acid Production by Rhizobium Strains from Indigofera
    African Journal of Microbiology Research Vol 3(1) pp.010-014, January, 2009 Available online http://www.academicjournals.org/ajmr ISSN 1996-0808 ©2009 Academic Journals Full Length Research Paper Studies on exopolysaccharide and indole acetic acid production by Rhizobium strains from Indigofera B. Saritha Kumari, M. Raghu Ram* and K. V. Mallaiah Department of Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar-522 510, Guntur, A. P., India. Accepted 9 October, 2008 Rhizobium strains were isolated from root nodules of five species of Indigofera viz., Indigofera trita, Indigofera linnaei, Indigofera astragalina, Indigofera parviflora and Indigofera viscosa on Yeast Extract Mannitol Agar (YEMA) medium. The strains were examined for production of acid, exopolysaccharide (EPS) and indole acetic acid (IAA) by utilizing 10 different carbon sources. The Rhizobium strains isolated from I. trita, I. parviflora and I. viscosa showed maximum growth on glucose, while those from I. linnaei and I. astragalina showed maximum growth on fructose and maltose, respectively. All the five strains produced acid, EPS and IAA in Yeast extract mannitol broth. Among the strains studied, maximum EPS production was observed with the strain isolated from I. parviflora and maximum IAA production was observed with the strain isolated from I. viscosa. Key words: Rhizobium, Indigofera species, exopolysaccharide, indole acetic acid, acid production. INTRODUCTION The legume-rhizobium interaction is the result of specific 2002) and was described as Rhizobium indigoferae sp. recognition of the host legume by Rhizobium. Various nov. Reports on the plant growth-promoting characteris- signal molecules that are produced by both Rhizobia and tics of Rhizobium species isolated from Indigofera are the legume confer the specificity (Phillips, 1991).
    [Show full text]
  • Isolation and Characterization of Salinity Tolerant Nitrogen Fixing Bacteria from Sesbania Sesban (L) Root Nodules
    Biocatalysis and Agricultural Biotechnology 21 (2019) 101325 Contents lists available at ScienceDirect Biocatalysis and Agricultural Biotechnology journal homepage: http://www.elsevier.com/locate/bab Isolation and characterization of salinity tolerant nitrogen fixing bacteria from Sesbania sesban (L) root nodules N. Nohwar, R.V. Khandare, N.S. Desai * Amity Institute of Biotechnology, Amity University Mumbai, Bhatan Panvel, Raigad, Mumbai Metropolitan Area, 410206, India ARTICLE INFO ABSTRACT Keywords: This work was aimed to isolate and characterize Rhizobia from Sesbania Sesban root nodules growing in different Sesbania sesban parts of Mumbai metropolitan area. Root nodules from these plant samples were harvested and 120 test isolates Rhizobium were obtained. A total of 75 obtained isolates were found to show poor absorption of Congo red when grown on High salt tolerance yeast extract mannitol agar with 2% NaCl. These isolates were further studied for their morphological and High pH tolerance biochemical characteristics along with Rhizobium leguminosarum as a reference culture. The isolates were found High temperature tolerance 16S rRNA sequencing to be gram negative rods and exhibited a rapid growth as evident from bromothymol blue test. Biochemical and Biofertilizers phenotypic characterization of all 75 isolates revealed them to be Rhizobium species. Furthermore, 8 represen­ tative isolates from 8 different Mumbai Metropolitan locations were used for genotypic analysis by 16S rRNA sequencing. All 8 isolates were shown to have inhibitory effect on growth when tested on different salt con­ centration. As the NaCl concentration was increased from 0.1 to 20%, the growth started declining. Further, at 30% concentration, an absolute growth inhibition was observed. The isolates exhibited noteworthy growth at pH � 6.0 to 11.0.
    [Show full text]
  • Bacteria in Agrobiology: Crop Productivity Already Published Volumes
    Dinesh K. Maheshwari, Meenu Saraf Abhinav Aeron Editors Bacteria in Agrobiology: Crop Productivity Bacteria in Agrobiology: Crop Productivity Already published volumes: Bacteria in Agrobiology: Disease Management Dinesh K. Maheshwari (Ed.) Bacteria in Agrobiology: Crop Ecosystems Dinesh K. Maheshwari (Ed.) Bacteria in Agrobiology: Plant Growth Responses Dinesh K. Maheshwari (Ed.) Bacteria in Agrobiology: Plant Nutrient Management Dinesh K. Maheshwari (Ed.) Bacteria in Agrobiology: Stress Management Dinesh K. Maheshwari (Ed.) Bacteria in Agrobiology: Plant Probiotics Dinesh K. Maheshwari (Ed.) Dinesh K. Maheshwari • Meenu Saraf • Abhinav Aeron Editors Bacteria in Agrobiology: Crop Productivity Editors Dinesh K. Maheshwari Meenu Saraf Dept. of Botany Microbiology University School of Sciences Gurukul Kangri University Dep. Microbiology and Biotechnology Haridwar (Uttarakhand), India Gujarat University Ahmedabad (Gujarat), India Abhinav Aeron Department of Biosciences DAV (PG) College Muzaffarnagar (Uttar Pradesh) India ISBN 978-3-642-37240-7 ISBN 978-3-642-37241-4 (eBook) DOI 10.1007/978-3-642-37241-4 Springer Heidelberg New York Dordrecht London Library of Congress Control Number: 2013942485 © Springer-Verlag Berlin Heidelberg 2013 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work.
    [Show full text]
  • Taxonomy of the Rhizobia: Current Perspectives
    British Microbiology Research Journal 4(6): 616-639, 2014 SCIENCEDOMAIN international www.sciencedomain.org Taxonomy of the Rhizobia: Current Perspectives Halima Berrada1 and Kawtar Fikri-Benbrahim1* 1Laboratory of Microbial Biotechnology, Faculty of Sciences and Technology, Sidi Mohammed Ben Abdellah University, P. O. Box 2202, Fez, Morocco. Authors’ contributions This work was carried out in collaboration between both authors. Both authors read and approved the final manuscript. Received 28th June 2013 th Review Article Accepted 6 February 2014 nd rti………… Published 22 February 2014 Article ABSTRACT The classification of rhizobia has been gone through a substantial change in recent years due to the addition of several new genera and species to this important bacterial group. Recent studies have shown the existence of a great diversity among nitrogen-fixing bacteria isolated from different legumes. Currently, more than 98 species belonging to 14 genera of α- and β- proteobacteria have been described as rhizobia. The genera Rhizobium, Mezorhizobium, Ensifer (formerly Sinorhizobium), Bradyrhizobium, Phyllobacterium, Microvirga, Azorhizobium, Ocrhobactrum, Methylobacterium, Devosia, Shinella (Class of α- proteobacteria), Burkholderia, Cupriavidus (formerly Ralstonia) (Class of β-proteobacteria) and some γ-proteobacteria, form the set of the bacteria known as legume’s symbionts. There is certainly much to discover, since only 23% of known legumes were identified specifically for symbiotic relationship up to date. The discovery of new symbionts associated with legumes is necessary to gain deep insight into the taxonomy of the rhizobia. A literature review of the currently recognized classification of rhizobia is presented in this paper. Keywords: Rhizobia; Legume; Proteobacteria; Taxonomy; Classification. ____________________________________________________________________________________________ *Corresponding author: Email: [email protected]; British Microbiology Research Journal, 4(6): 616-639, 2014 1.
    [Show full text]
  • Universidade Federal Rural Do Rio De Janeiro Instituto De Florestas Curso De Graduação Em Engenharia Florestal
    Universidade Federal Rural do Rio de Janeiro Instituto de Florestas Curso de Graduação em Engenharia Florestal Especificidade hospedeira de estirpes de rizóbio para leguminosas florestais Fernando Soares Gonçalves Seropédica, RJ Julho, 2009 Especificidade hospedeira de estirpes de rizóbio para leguminosas florestais Fernando Soares Gonçalves Sob orientação do pesquisador Sergio Miana de Faria Monografia apresentada ao Curso de Engenharia Florestal, como requisito para a obtenção do título de Engenheiro Florestal, Instituto de Florestas da Universidade Federal Rural do Rio de Janeiro. Seropédica, RJ Julho, 2009 II Especificidade hospedeira de estirpes de rizóbio para leguminosas florestais Fernando Soares Gonçalves Aprovada em 06 / 07 / 2009 ______________________________________________________ Sergio Miana de Faria Pesquisador-Embrapa Agrobiologia (orientador) _____________________________________________________ Gustavo Ribeiro Xavier Pesquisador-Embrapa Agrobiologia _____________________________________________________ Claudia Pozzi Jantalia Pesquisadora-Embrapa Agrobiologia III “No final tudo da certo. Se ainda não deu certo é porque não acabou.” Zélia Cardoso de Melo IV AGRADECIMENTOS Agradeço aos meus pais Vicente e Maria que sempre me apoiaram em todos os momentos, a minha irmã Liliane e ao meu irmão Leandro, que estarão sempre comigo mesmo que a distancia seja grande. À minha segunda família que me deu muito carinho e me trataram como um filho e sempre me apoiando. À minha sogrinha Selma, ao sogro Helio e aos cunhados Eduardo e Bruno. À minha grande amiga e noiva Kelly pelo carinho, apoio, dedicação e companheirismo dedicado todos os dias. À Universidade Federal Rural do Rio de Janeiro pelo suporte para o meu desenvolvimento como cidadão e profissional. À Embrapa Agrobiologia e a toda a minha família do Laboratório de Leguminosas pelos dias de alegria e risadas proporcionadas, principalmente nos dias de stress de final de período.
    [Show full text]
  • Characterisation of Rhizobia and Studies on N₂ Fixation of Common
    Lincoln University Digital Thesis Copyright Statement The digital copy of this thesis is protected by the Copyright Act 1994 (New Zealand). This thesis may be consulted by you, provided you comply with the provisions of the Act and the following conditions of use: you will use the copy only for the purposes of research or private study you will recognise the author's right to be identified as the author of the thesis and due acknowledgement will be made to the author where appropriate you will obtain the author's permission before publishing any material from the thesis. Characterisation of rhizobia and studies on N2 fixation of common weed legumes in New Zealand ___________________________________ A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy in Molecular Microbiology by Wendy Ying Ying Liu _______________________________ Lincoln University 2014 Abstract of a thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy (Molecular Microbiology) Characterisation of rhizobia and studies on N2 fixation of common weed legumes in New Zealand by Wendy Ying Ying Liu Most legume species can fix atmospheric N2 via symbiotic bacteria (collectively termed rhizobia) in nodules on their roots, thus allowing them to colonise marginal land with low soil N availability. Over the last 150 years, over 100 legume species from different continents have become naturalised in NZ and many of these are now common weeds. The major objective of this study was to genotypically characterise rhizobial isolates which produce N2-fixing nodules on common weed legumes in NZ soils via phylogenetic analyses of 16S rRNA, recA, nifH, nodA and/or nodC gene sequences to establish their identity, diversity and presumptive origin(s).
    [Show full text]