Product Introduction 2 occurs in response to the concentration and the the and concentration the to response in light occurs polarized linearly of plane the of rotation Optical Measurement Principle from feedback 3 its now released has development, HORIBA with product HORIBA’s ongoing with r together and customers introduction, suga its Since icals, 1992. in SEPA-200, the ceut a m polarimeter r first a its ph offered HORIBA s a such s foodindustry. the and manufacturing, ield f n i d se u substances active optically other and hormones, alkaloids, vitamins, acids, amino , of purity or concentration the for measuring products indispensable are Polarimeters Introduction 村田 駿介 Shunsuke MURATA コンパクト自動旋光計 SEPA-500 Compact Automatic Polarimeter SEPA-500 Product Introduction and research, the SEPA-500 was developed to perform measurements. easy and quick, accurate, highly perform to developed was SEPA-500 the research, and technologies latest the SEPA-500. Using the polarimeter English Edition No.16 February 2012 English Edition No.16February rd generation generation the light enters a photo diode that converts the intensity to to intensity the converts that diode aphoto enters light the Finally, rotation. of angle the detects which “Analyzer,” the as known for , second a to rotator then and Faraday modulation a passed has enters it cell, light the sample the After through cell. the of path light and concentration, activity, optical the to response in rotates plane The light sample. liquid with cell the enters polarized only so polarized linearly is that light f source o wavelength fixed n a o with i begins t c u measurement The d o r p l a i r in SEPA-500 is shown configuration t s u d n i n i l o r t n foods. and o pharmaceuticals c s s e c o and r p or quality control quality for used product be can polarimeters measure therefore to parameter a as used be can rotation . optical from measured be can activity optical and concentration the and active, optically generally are acids amino active and Sugars materials. optically through passing light of characteristics Yoshiaki TOGAWAYoshiaki MURATA, Takeshi YAMANAKA, Shunsuke OHNISHI, Tomoyuki SHIMA, Mitsuko to right) (left Row Front Yuki ISHII HIRAI, Chie FUJIWARA, Shingo MATSUME, Yoshitaka IDA, Yoshihiko to right) (left Row Middle Toshiya ITO SAIJO, Yutaka UKON, Juichiro YONEZAWA, Hyosuke to right) (left Row Back Tasuo IGUSHI left the On Figure 2 Figure . Technical Reports Product Introduction

Compact Automatic Polarimeter SEPA-500 コンパクト自動旋光計 SEPA-500

Shunsuke MURATA

Figure 1 SEPA-500 村田 駿介

SEPA-500

Light source Photo Diode (LED) Sample cell Interference filter Faraday cell Lens Lens Hollow shaft motor Analyzer Polarizer

Figure 2 SEPA-500 configuration

an electrical signal. High Speed and High Resolution Measurement The rotating Analyzer automatically controls the angle to minimize the detected intensity at all times. That is, the Precision and speed of measurement depends on the polarization plane angle of the analyzer is controlled to be control mechanism of the rotating Analyzer. orthogonal to the light, which is rotated while passing In the previous SEPA-300 model, HORIBA had achieved through sample. The optical rotation obtained is the a resolution of 5/10000 degrees using a stepping motor difference between the rotation angle of a sample filled and a worm gear in the rotating Analyzer. cell and that of the empty cell. The Faraday rotator is made of a transparent material The SEPA-500 uses a new hollow shaft motor. The surrounded by a Faraday coil. The polarization plane of hollow shaft motor has no shaft or any moving parts in its the light traveling inside is rotated when a magnetic field rotation axis so the beam can pass through the Analyzer is applied, using a current in the coil. This is called the which is set on the rotation axis. This method removes Faraday effect. Using this Faraday effect, the direction of angle measurement errors, due to mechanical components rotation of the polarization plane is detected. The such as the coupling and the worm gear, used in earlier resolution of the measurement angle is improved by models. vibrating the polarization plane to optically null the Additionally, using digital electronics, the control measurement. algorithm was optimized for smooth and stable positioning of the rotating Analyzer, while keeping a low backlash. With these improvements, a maximum rotation

English Edition No.16 February 2012 3 Product Introduction 4 wavelength width of the LED is larger than that of Na Na of that than larger is LED the of width wavelength the As source. light the as LED the has SEPA-500 The Maintenance Free of Light Source the for aflowcell. of using samples also monitoring continuous but rotation, optical large with samples for useful only not is measurement resolution high and speed high This resolution. angle more fivetimes rotation has and speed model’s previous the is than higher the times performance twelve This achieving worldwide. is it performance achieved, highest were of degrees, resolution 1/10000 angle an and degrees/second 60 of speed Product Introduction Specifications *2: *1: Figure 3 SEPA-500 specifications 3SEPA-500 Figure Measurement repeatability Measurement accuracy Range ofmeasurementangles Maximum celllength Sensor Measurement wavelength Polarizer Light source Weight circulation connection Thermostatic water External dimensions External connection Data storage Data processing Measurement items Input method Display temperatures Range ofmeasurement Temperature measurementpoints Measurement method Maximum responsespeed Minimum displayangle Someunitswillnotoperat The pr English Edition No.16 February 2012 English Edition No.16February oduct displa ys usedinthiscatalogar e withFA 13 inte Hose: Maximum headpr Maximum Ext C Angle ofro T Co 0-99.9 C Ce null method S 60 /sec. 0.000 ±0.002 ±0.002 (atlessthan1); concentration, sug suga ±90 1 Phot 589 nm(equiva Po LED Ext statistical (mean,SD)pr Body: Ether USB memory T1 ouch scr 00 mm ymmetr ontinuous aut 6 syst kg er lar lor LCD; ll holder/insidecell er r nal pr nal diamet odiode r cont izing f nal pr net 1 490 (W)×250(D)×200(H)mm Ext em memor e composite inte ical angleoscillationoptical een er ent (int wa tation, specif inte 5.7 ilm r: nal diamet 1 te (*1) 06 (W)×1 r (optional),ex er r fl omatic measur y pr lent to s. er :

ow

ar pur 7 mm essure Ac oducts. national standar ±0.2%(at1ormor Compact Automatic Polarimeter SEPA-500 tual display : 81 (D)×1 12 sodiumD-line) er ic ro it L/min. ocessing : y : 11 50 kP tation, mm; 07 (H)mm te s ma ement, r a nal

y diff d), e) er . earlier models. This light source is also practically use. general practically in also 10years than is more for last will it as source free maintenance light This in models. used lamp Na earlier the to compared hours 20000 over of lifetime longer a has LED The nm. 2.5 to maximum) half at width spectral (full FWHM and 589nm to wavelength central the control to used is filter interference an lamp, previous model. previous the of volume and kg. weight 13 the half weight a approximately is external and This H200 with D250 W490 of design dimensions saving nal space a exter has with SEPA-500 SEPA-300, the kg, 35 weight a the and H270 W565D445 of to dimensions compared hen W axis the system. of optical the stability improves also but is weight and size the frame reduces motor only not the This components. which optical in between sandwiched very system a has optical SEPA-500 the simple Additionally, mechanism. gear worm traditional the than rather motor a hollow shaft of ausing Na and lamp, instead source LED light an using as such system, optical the in advances the to possible due was This size. compact a and footprint smaller a has also SEPA-500 the performance, in advances the to addition In Compact and Light Weight use with aPC. with use for drive thumb USB a to saved also and printer, optional impact an on printed screen, on displayed be can data measurement the Measured disk. removable a screen, on stored be can conditions setup screen. condition touch the the From using purity, or concentration standard), (international content sugar rotation, specific of rotation, angle to mode display the switch can user The touch a color display. LCD screen using by improved also was interface user The Devised Operativeness Technical Reports

Product Introduction Compact Automatic Polarimeter SEPA-500 Optical rotation measurement to be made without the need for a thermostat bath, reducing bench space requirements and the possibilities of a water leak.

Summary

The SEPA-500 has achieved high speed / high resolution measurements by using a hollow shaft motor and incorporating an LED to eliminate light source maintenance. A touch panel display was adopted to enhance the user interface. The (optional) Peltier type temperature control cell unit maintains a constant temperature while eliminating water leaks. The SEPA-500 has been developed as compact and light weight analyzer and we hope that customers will enjoy the enhanced user interface and the simplicity of operation.

Figure 4 Measurement results screen and condition setting and input screen

Temperature Control Cell Unit with Peltier Effect

Temperature control is important for the optical rotation measurements as unstable temperatures will generate erroneous data. In the earlier models, the solution was to use circulating water from connected thermostat bath. In SEPA-500, a Peltier type temperature control cell unit can be installed to cool the sample chamber (Figure 5). This permits the

Figure 5 Peltier type temperature control cell unit in the sample chamber

English Edition No.16 February 2012 5