The Sandy Beach Environment
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The Green Fluorescent Protein
P1: rpk/plb P2: rpk April 30, 1998 11:6 Annual Reviews AR057-17 Annu. Rev. Biochem. 1998. 67:509–44 Copyright c 1998 by Annual Reviews. All rights reserved THE GREEN FLUORESCENT PROTEIN Roger Y. Tsien Howard Hughes Medical Institute; University of California, San Diego; La Jolla, CA 92093-0647 KEY WORDS: Aequorea, mutants, chromophore, bioluminescence, GFP ABSTRACT In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrin- sically fascinating and tremendously valuable. High-resolution crystal structures of GFP offer unprecedented opportunities to understand and manipulate the rela- tion between protein structure and spectroscopic function. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories. CONTENTS NATURAL AND SCIENTIFIC HISTORY OF GFP .................................510 Discovery and Major Milestones .............................................510 Occurrence, Relation to Bioluminescence, and Comparison with Other Fluorescent Proteins .....................................511 PRIMARY, SECONDARY, TERTIARY, AND QUATERNARY STRUCTURE ...........512 Primary Sequence from -
Morphology of Two Deep-Sea Olivella from the Southwestern Atlantic, with a Record of a Radula-Less Olivellinae Species (Neogastropoda: Olivoidea: Olividae)
Archiv für Molluskenkunde 150 (1) 31–43 Frankfurt am Main, 30 June 2021 Morphology of two deep-sea Olivella from the southwestern Atlantic, with a record of a radula-less Olivellinae species (Neogastropoda: Olivoidea: Olividae) Alexandre Dias Pimenta1 & Luiz Ricardo Lopes Simone2 1 Departamento de Invertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro, Quinta da Boa Vista, São Cristóvão, 20940-040, Rio de Janeiro, Brazil ([email protected]). https://orcid.org/0000-0001-7001-5820 2 Museu de Zoologia da Universidade de São Paulo, Avenida Nazaré, 481, Ipiranga, 04263-000, São Paulo, Brazil ([email protected]). https://orcid.org/0000-0002-1397-9823 • Corresponding author: A.D. Pimenta. Abstract. Two deep-sea species from southeast Brazil, originally assigned to different subgenera ofOli - vella, are anatomically described. Olivella (Olivina) klappenbachi and Olivella (Anasser) careorugula, both described by Absalão & Pimenta (2003), present typical Olivellinae anatomy, with internal absorp tion of the shell wall and a non-spiralized visceral mass, the absence of a valve of Leiblein and gland of Leiblein, and a large cuticularized and muscular stomach. Olivella klappenbachi presents the typical radular morphology of Olivellinae, while Olivella careorugula lacks a radula and odontophore, which is unique among known olivids. A well-founded phylogenetic classification of Olivella at the generic/ subgeneric level is still lacking, and the numerous proposed subgenera are mostly based on the structure of the pillar. A broader taxonomic -
Alegria, Santa Barbara County, California May 26, 2001 Species List
PISCO Coastal Biodiversity Survey University of California Santa Cruz http://cbsurveys.ucsc.edu Please note: The information listed below is provided for your convenience. We ask that you please contact the SWAT Team ([email protected]) prior to using this information for any purpose. We make this request to: 1. Reduce redundancy; we may be currently working on projects that involve this information. 2. We would like to be informed of and involved in projects developed using this information. We have been careful to voucher any organisms that were difficult to identify in the field so that more detailed evaluation could be done in the lab. We are therefore confident that the identification of organisms listed below is reliable with the caveat that some sponges and tunicates are very difficult to identify to species without detailed histological evaluation, which we have not done. The number of cases where this could have been a problem is very small. For more information please visit our website above or link directly to our protocols at: http://cbsurveys.ucsc.edu/sampling/images/dataprotocols.pdf Alegria, Santa Barbara County, California May 26, 2001 Species list: Acanthinucella spp Lepidochitona dentiens Acrosorium ciliolatum Lepidochitona hartwegii Alia spp Littorina keenae Anthopleura elegantissima Littorina plena/scutulata Anthopleura sola Lottia austrodigitalis/digitalis Balanus glandula Lottia limatula Blue green algae Lottia paradigitalis/strigatella Bossiella spp Lottia pelta Brachidontes/Septifer spp Lottia scabra/conus Calliarthron -
ECOLOGÍA DE “Oliva Bewleyi” (Marrat, 1870 )
ECOLOGÍA DE “Oliva bewleyi” (Marrat, 1870) (MOLLUSCA: GASTROPODA:OLIVIDAE) EN BAHÍA CONCHA, PARQUE NACIONAL NATURAL TAYRONA (PNNT), CARIBE COLOMBIANO DIANA CAROLINA TORRES PALACIO UNIVERSIDAD DE BOGOTÁ JORGE TADEO LOZANO FACULTAD DE CIENCIAS NATURALES PROGRAMA DE BIOLOGÍA MARINA BOGOTÁ D. C. 2008 ECOLOGÍA DE “Oliva bewleyi” (Marrat, 1870) (MOLLUSCA: GASTROPODA:OLIVIDAE) EN BAHÍA CONCHA, PARQUE NACIONAL NATURAL TAYRONA (PNNT), CARIBE COLOMBIANO DIANA CAROLINA TORRES PALACIO Director Juan Manuel Díaz Merlano Dr. rer. Nat Codirector Oscar Delgadillo Garzón Biólogo Marino, UJTL Coasesor Adolfo Sanjuán Muñoz Biólogo Marino, M.Sc. UNIVERSIDAD DE BOGOTÁ JORGE TADEO LOZANO FACULTAD DE CIENCIAS NATURALES PROGRAMA DE BIOLOGÍA MARINA BOGOTÁ D. C. 2008 ii Nota de Aceptación ____________________________ ____________________________ ____________________________ ____________________________ ____________________________ ____________________________ ____________________________ Firma del presidente del jurado ____________________________ Firma del jurado ____________________________ Firma del jurado iii Este trabajo está dedicado a mi familia por su amor, apoyo y paciencia. Y en este momento de mi vida, a Juan y a mi bebe Solé, por darle luz a mi vida. La perseverancia por alcanzar mi sueño de mar. iv AGRADECIMIENTOS Agradezco al creador del universo por darme vida y la oportunidad de poder disfrutar con toda la energía y amor, este trabajo de grado y en especial, de poder bucear mucho, del mar, la playa y el grupo de moluscos A mi familia, agradezco su apoyo incondicional, su paciencia, sabiduría, oportunidades y sobre todo su amor. Por que gracias a ellos he podido darme cuenta de mis errores, recuperar la confianza y así continuar con valor… Juan Manuel Díaz, más que un director ha sido como un papá, por su apoyo profesional y su amistad, por su gran paciencia y sobre todo por creer en mi y darme siempre la oportunidad. -
Bioluminescence and Fluorescence of Three Sea Pens in the North-West
bioRxiv preprint doi: https://doi.org/10.1101/2020.12.08.416396; this version posted December 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Bioluminescence and fluorescence of three sea pens in the north-west Mediterranean sea Warren R Francis* 1, Ana¨ısSire de Vilar 1 1: Dept of Biology, University of Southern Denmark, Odense, Denmark Corresponding author: [email protected] Abstract Bioluminescence of Mediterranean sea pens has been known for a long time, but basic parameters such as the emission spectra are unknown. Here we examined bioluminescence in three species of Pennatulacea, Pennatula rubra, Pteroeides griseum, and Veretillum cynomorium. Following dark adaptation, all three species could easily be stimulated to produce green light. All species were also fluorescent, with bioluminescence being produced at the same sites as the fluorescence. The shape of the fluorescence spectra indicates the presence of a GFP closely associated with light production, as seen in Renilla. Our videos show that light proceeds as waves along the colony from the point of stimulation for all three species, as observed in many other octocorals. Features of their bioluminescence are strongly suggestive of a \burglar alarm" function. Introduction Bioluminescence is the production of light by living organisms, and is extremely common in the marine environment [Haddock et al., 2010, Martini et al., 2019]. Within the phylum Cnidaria, biolumiescence is widely observed among the Medusazoa (true jellyfish and kin), but also among the Octocorallia, and especially the Pennatulacea (sea pens). -
Section 3.5 Marine Biology
3.5 MARINE BIOLOGY 3.5.1 Introduction 3.5.1.1 Definition of Resource For purposes of this EIS/OEIS, marine biological resources are defined as marine flora and fauna and habitats that they occupy, occurring within the Point Mugu Sea Range, Mugu Lagoon, and the intertidal and nearshore environment of San Nicolas Island and Point Mugu. This section specifically addresses marine invertebrates and flora. Fish and sea turtles are addressed in Section 3.6, marine mammals are addressed in Section 3.7, and seabirds are addressed in Section 3.8. Threatened and endangered species, as defined by the U.S. Fish and Wildlife Service (USFWS), are also addressed. Species that are federally listed are afforded a degree of regulatory protection, which entails a permitting process including specific mitigation measures for any allowable (incidental) impacts on the species. Species that are proposed to be listed by the USFWS are treated similarly to listed species by that agency; recommendations of the USFWS, however, are advisory rather than mandatory in the case of proposed species. A federally listed endangered species is defined as any species, including subspecies, that is “in danger of extinction throughout all or a significant portion of its range.” A federally listed threatened species is defined as any species “likely to become an endangered species within the foreseeable future throughout all or a significant portion of its range.” “Proposed” endangered or threatened species are those species for which a proposed regulation has been published in the Federal Register, but a final rule has not yet been issued. 3.5.1.2 Regional Setting The Sea Range straddles the ocean off Point Conception which is considered a major geographic feature that affects marine biological diversity. -
OREGON ESTUARINE INVERTEBRATES an Illustrated Guide to the Common and Important Invertebrate Animals
OREGON ESTUARINE INVERTEBRATES An Illustrated Guide to the Common and Important Invertebrate Animals By Paul Rudy, Jr. Lynn Hay Rudy Oregon Institute of Marine Biology University of Oregon Charleston, Oregon 97420 Contract No. 79-111 Project Officer Jay F. Watson U.S. Fish and Wildlife Service 500 N.E. Multnomah Street Portland, Oregon 97232 Performed for National Coastal Ecosystems Team Office of Biological Services Fish and Wildlife Service U.S. Department of Interior Washington, D.C. 20240 Table of Contents Introduction CNIDARIA Hydrozoa Aequorea aequorea ................................................................ 6 Obelia longissima .................................................................. 8 Polyorchis penicillatus 10 Tubularia crocea ................................................................. 12 Anthozoa Anthopleura artemisia ................................. 14 Anthopleura elegantissima .................................................. 16 Haliplanella luciae .................................................................. 18 Nematostella vectensis ......................................................... 20 Metridium senile .................................................................... 22 NEMERTEA Amphiporus imparispinosus ................................................ 24 Carinoma mutabilis ................................................................ 26 Cerebratulus californiensis .................................................. 28 Lineus ruber ......................................................................... -
The Diet and Predator-Prey Relationships of the Sea Star Pycnopodia Helianthoides (Brandt) from a Central California Kelp Forest
THE DIET AND PREDATOR-PREY RELATIONSHIPS OF THE SEA STAR PYCNOPODIA HELIANTHOIDES (BRANDT) FROM A CENTRAL CALIFORNIA KELP FOREST A Thesis Presented to The Faculty of Moss Landing Marine Laboratories San Jose State University In Partial Fulfillment of the Requirements for the Degree Master of Arts by Timothy John Herrlinger December 1983 TABLE OF CONTENTS Acknowledgments iv Abstract vi List of Tables viii List of Figures ix INTRODUCTION 1 MATERIALS AND METHODS Site Description 4 Diet 5 Prey Densities and Defensive Responses 8 Prey-Size Selection 9 Prey Handling Times 9 Prey Adhesion 9 Tethering of Calliostoma ligatum 10 Microhabitat Distribution of Prey 12 OBSERVATIONS AND RESULTS Diet 14 Prey Densities 16 Prey Defensive Responses 17 Prey-Size Selection 18 Prey Handling Times 18 Prey Adhesion 19 Tethering of Calliostoma ligatum 19 Microhabitat Distribution of Prey 20 DISCUSSION Diet 21 Prey Densities 24 Prey Defensive Responses 25 Prey-Size Selection 27 Prey Handling Times 27 Prey Adhesion 28 Tethering of Calliostoma ligatum and Prey Refugia 29 Microhabitat Distribution of Prey 32 Chemoreception vs. a Chemotactile Response 36 Foraging Strategy 38 LITERATURE CITED 41 TABLES 48 FIGURES 56 iii ACKNOWLEDGMENTS My span at Moss Landing Marine Laboratories has been a wonderful experience. So many people have contributed in one way or another to the outcome. My diving buddies perse- vered through a lot and I cherish our camaraderie: Todd Anderson, Joel Thompson, Allan Fukuyama, Val Breda, John Heine, Mike Denega, Bruce Welden, Becky Herrlinger, Al Solonsky, Ellen Faurot, Gilbert Van Dykhuizen, Ralph Larson, Guy Hoelzer, Mickey Singer, and Jerry Kashiwada. Kevin Lohman and Richard Reaves spent many hours repairing com puter programs for me. -
Sand Beach Monitoring at Channel Islands National Park, 2007-2012
National Park Service U.S. Department of the Interior Natural Resource Stewardship and Science Sand Beach Monitoring at Channel Islands National Park 2007-2012 Natural Resource Report NPS/MEDN/NRR—2015/1049 ON THE COVER Clockwise from Upper left: wrack on China Camp Beach, Old Ranch House Canyon Lagoon, elephant seals on China Camp Beach, Jacob Elliott and Stephen Whitaker conducting core samples on upper beach transect at Water Canyon, Santa Rosa Island. Photograph by: Dan Richards Sand Beach Monitoring at Channel Islands National Park 2007-2012 Natural Resource Report NPS/MEDN/NRR—2015/1049 Daniel V. Richards and Stephen G. Whitaker National Park Service Channel Islands National Park 1901 Spinnaker Drive Ventura, CA 93001 October 2015 U.S. Department of the Interior National Park Service Natural Resource Stewardship and Science Fort Collins, Colorado The National Park Service, Natural Resource Stewardship and Science office in Fort Collins, Colorado, publishes a range of reports that address natural resource topics. These reports are of interest and applicability to a broad audience in the National Park Service and others in natural resource management, including scientists, conservation and environmental constituencies, and the public. The Natural Resource Report Series is used to disseminate comprehensive information and analysis about natural resources and related topics concerning lands managed by the National Park Service. The series supports the advancement of science, informed decision-making, and the achievement of the National Park Service mission. The series also provides a forum for presenting more lengthy results that may not be accepted by publications with page limitations. All manuscripts in the series receive the appropriate level of peer review to ensure that the information is scientifically credible, technically accurate, appropriately written for the intended audience, and designed and published in a professional manner. -
An Invitation to Monitor Georgia's Coastal Wetlands
An Invitation to Monitor Georgia’s Coastal Wetlands www.shellfish.uga.edu By Mary Sweeney-Reeves, Dr. Alan Power, & Ellie Covington First Printing 2003, Second Printing 2006, Copyright University of Georgia “This book was prepared by Mary Sweeney-Reeves, Dr. Alan Power, and Ellie Covington under an award from the Office of Ocean and Coastal Resource Management, National Oceanic and Atmospheric Administration. The statements, findings, conclusions, and recommendations are those of the authors and do not necessarily reflect the views of OCRM and NOAA.” 2 Acknowledgements Funding for the development of the Coastal Georgia Adopt-A-Wetland Program was provided by a NOAA Coastal Incentive Grant, awarded under the Georgia Department of Natural Resources Coastal Zone Management Program (UGA Grant # 27 31 RE 337130). The Coastal Georgia Adopt-A-Wetland Program owes much of its success to the support, experience, and contributions of the following individuals: Dr. Randal Walker, Marie Scoggins, Dodie Thompson, Edith Schmidt, John Crawford, Dr. Mare Timmons, Marcy Mitchell, Pete Schlein, Sue Finkle, Jenny Makosky, Natasha Wampler, Molly Russell, Rebecca Green, and Jeanette Henderson (University of Georgia Marine Extension Service); Courtney Power (Chatham County Savannah Metropolitan Planning Commission); Dr. Joe Richardson (Savannah State University); Dr. Chandra Franklin (Savannah State University); Dr. Dionne Hoskins (NOAA); Dr. Charles Belin (Armstrong Atlantic University); Dr. Merryl Alber (University of Georgia); (Dr. Mac Rawson (Georgia Sea Grant College Program); Harold Harbert, Kim Morris-Zarneke, and Michele Droszcz (Georgia Adopt-A-Stream); Dorset Hurley and Aimee Gaddis (Sapelo Island National Estuarine Research Reserve); Dr. Charra Sweeney-Reeves (All About Pets); Captain Judy Helmey (Miss Judy Charters); Jan Mackinnon and Jill Huntington (Georgia Department of Natural Resources). -
Emily Sue Stafford
Measuring and Interpreting Predation on Gastropod Shells by Emily Sue Stafford A thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy Department of Earth and Atmospheric Sciences University of Alberta © Emily Sue Stafford, 2014 ABSTRACT This dissertation focuses on problems and progress in studying crushing predation on gastropods in the Modern and the fossil record. Although crushing predation tends to be destructive, it is possible to gather data on crushing predation from multiple angles. Chapter 2 applies an ichnotaxonomic name, Caedichnus, to the trace created by peeling crab predators. Chapter 3 the relationship between shell repair frequency and predation mortality in a modern gastropod community. In this case, repair frequency was likely a direct product of variation in predator abundance and strength. Chapter 4 focused on hermit crabs, an organism that inhabits gastropod shells and exposes those shells to predation even after the original gastropod inhabitant has died. The predatory crabs showed no preference for snail or hermit crab prey, which may mean that hermit crab habitation does not significantly alter the crab-on-snail predation patterns present in a shell assemblage. Chapter 5 expanded on previous work by the author, using a method by G.J. Vermeij to estimate crushing predation in a gastropod assemblage even when individual instances of predatory damage cannot be identified. Vermeij Crushing Analysis (VCA) uses drilled shells to establish a baseline of taphonomic damage in a shell assemblage; the chapter refines and examines this method more deeply, in addition to applying the method to compare predation on modern and fossil gastropod shell assemblages. -
An Annotated Checklist of the Marine Macroinvertebrates of Alaska David T
NOAA Professional Paper NMFS 19 An annotated checklist of the marine macroinvertebrates of Alaska David T. Drumm • Katherine P. Maslenikov Robert Van Syoc • James W. Orr • Robert R. Lauth Duane E. Stevenson • Theodore W. Pietsch November 2016 U.S. Department of Commerce NOAA Professional Penny Pritzker Secretary of Commerce National Oceanic Papers NMFS and Atmospheric Administration Kathryn D. Sullivan Scientific Editor* Administrator Richard Langton National Marine National Marine Fisheries Service Fisheries Service Northeast Fisheries Science Center Maine Field Station Eileen Sobeck 17 Godfrey Drive, Suite 1 Assistant Administrator Orono, Maine 04473 for Fisheries Associate Editor Kathryn Dennis National Marine Fisheries Service Office of Science and Technology Economics and Social Analysis Division 1845 Wasp Blvd., Bldg. 178 Honolulu, Hawaii 96818 Managing Editor Shelley Arenas National Marine Fisheries Service Scientific Publications Office 7600 Sand Point Way NE Seattle, Washington 98115 Editorial Committee Ann C. Matarese National Marine Fisheries Service James W. Orr National Marine Fisheries Service The NOAA Professional Paper NMFS (ISSN 1931-4590) series is pub- lished by the Scientific Publications Of- *Bruce Mundy (PIFSC) was Scientific Editor during the fice, National Marine Fisheries Service, scientific editing and preparation of this report. NOAA, 7600 Sand Point Way NE, Seattle, WA 98115. The Secretary of Commerce has The NOAA Professional Paper NMFS series carries peer-reviewed, lengthy original determined that the publication of research reports, taxonomic keys, species synopses, flora and fauna studies, and data- this series is necessary in the transac- intensive reports on investigations in fishery science, engineering, and economics. tion of the public business required by law of this Department.