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SIRT5 Antibody (Pab) 21.10.2014SIRT5 antibody (pAb) Chicken Anti -Human/Mouse SIRT5 (Sirtuin -5, SIR2L5) Instruction Manual Catalog Number PK-AB718-5771 Synonyms SIRT5 Antibody: Sirtuin 5, NAD-dependent deacetylase sirtuin-5, SIR2L5 Description The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension. Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p. Sirtuins are NAD ⁺-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. SIRT5 localizes to mitochondria, deacetylates carbamoyl phosphate synthetase 1, and is involved in the regulation of the urea cycle. Quantity 100 µg Source / Host Chicken Immunogen SIRT5 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SIRT5. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse Specificity Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. SIRT5 antibody can be used for detection of SIRT5 by Western blot at 1 - 2 µg/mL. Images Available upon request. References Salminen A. SIRT1: regulation of longevity via autophagy. Cell Signal2009; 21:1356-60. Afshar G and Murnane JP. Characterization of a human gene with sequence homology to Saccharomyces cerevisiae Sir 2. Gene1999; 234:161-8. Guarente L. Sirtuins as potential targets for metabolic syndrome. Nature2006; 444:868-74. Vaziri H, Dessain SK, Ng Eaton E, et al. hSIR2 (SIRT1) functions as an NAD-dependent p53 deacetylase. Cell2001; 107:149-59. Images Available upon request. Related Products Cat. No. PK-AB718-5771P - SIRT5 Peptide Cat. No. PK-AB718-1304 - Human Liver Tissue Lysate FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES. PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014 .
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