Physico-Chemical Properties and GCMS Analyses of Indigenous Rice Bran and Mustard Seed Oils and Their Blends
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Research Article ISSN: 2574 -1241 DOI: 10.26717/BJSTR.2021.34.005620 Physico-Chemical Properties and GCMS Analyses of Indigenous Rice Bran and Mustard Seed Oils and their Blends Mst. Sarmina Yeasmin1*, G.M. Masud Rana1, Tahmina Akhtar Chowdhury1, Lailatul Ferdousi2, Md. Mahmudur Rahman1 and Md. Moktadir Rahman1 1Oils, Fats and Waxes Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Rajshahi Laboratory, Bangladesh 2Applied Zoology Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Rajshahi Laboratory, Bangladesh *Corresponding author: Industrial Research (BCSIR), Rajshahi Laboratory, Bangladesh Mst. Sarmina Yeasmin, Oils, Fats and Waxes Research Division, Bangladesh Council of Scientific and ARTICLE INFO ABSTRACT Received: acid ratio. Indigenous Rice Bran Oil (RBO) and Mustard Seed Oil (MO) were mixed in the Published: February March 30, 18, 2021 2021 Blended edible oils were formulated to enrich fatty acid profile with balance fatty andratio peroxide of 60:40, value 70:30 of andthe 90:10single (RBO:MO).vegetable oils Moisture and their content, blends density, were determined. specific gravity, Fat- Citation: Mst Sarmina Yeasmin, G M tyrefractive acid composition index, viscosity, such freeas Saturated fatty acid, Fatty acid Acidvalue, (SFA), iodine Mono-Unsaturated value, unsaponifiable Fatty matter Acid Masud Rana, Lailatul Ferdousi, Tahmina (MUFA), Poly-Unsaturated Fatty Acid (PUFA) of all oils were analyzed by GCMS. GCMS Akhtar Chowdhury, Md Mahmudur Rahman, Md Moktadir Rahman. Physico- Chemical Properties and GCMS Analyses analysis of rice bran oil identified 6 fatty acids with ratio SFA:MUFA:PUFA=1.43:1.46:1.0- of Indigenous Rice Bran and Mustard Seed and mustard seed oil identified 8 fatty acids with ratio SFA:MUFA:PUFA=1.72:1:2.06. All Oils and their Blends. Biomed J Sci & Tech blended oils revealed 9 fatty acids with ratio SFA:MUFA:PUFA=1.24:2.1:1.0;SFA:MUFA:P Res 34(5)-2021. BJSTR. MS.ID.005620. MO)UFA=1.34:2.56:1.0andSFA:MUFA:PUFA=1.2:1.6:1.0; ratio was near World Health Organization (WHO) for 60:40, guidelines. 70:30 According and 90:10 to (RBO:WHO MO) respectively. Among three blendings, SFA:MUFA:PUFA=1.2:1.6:1.0 for 90:10 (RBO: Keywords: MUFA and PUFA respectively. The results suggest that 90:10 (RBO:MO) blended oil can beand good other alternative health agencies, oil for nutritionallyedible purposes superior with oilimprove must fall nutrition in the ratioas well of 1:1-3:1;as desirable SFA; Unsaturation Blended Oil; Rice Bran Oil; Mustard Oil; Fatty Acids; Saturation and physico-chemical properties. Abbreviations: RBO: Rice Bran Oil; MSO: Mustard Seed Oil; SFA: Saturated Fatty Acid; MUFA: Mono-Unsaturated Fatty Acid; PUFA: Poly-Unsaturated Fatty Acid; LDL-C: Low Density Lipoprotein Cholesterol; FFA: Free Fatty Acid; AV: Acid Value Introduction gamma-oryzanol, high levels of phytosterols and tocols [1,2]. Due to the presence of micronutrients in RBO, the value of other edible vegetable oils can be nutritionally improved by mixing with RBO. different fatty acids. But high intakes of Saturated Fatty Acids (SFA) Vegetable oil plays a variety of in human nutrition due to its Choudhary, et al. [3] stated that blending of non-conventional RBO increase Low Density Lipoprotein Cholesterol (LDL-C) as well as cardiovascular diseases. On the other hand, unsaturated fatty acids stable cooking oil that reduces the demand for traditional oils. On have the opposite effects. To combat this fatty acid ratio problem, with traditional oils (sunflower oil, canola oil etc.) can result in a the other hand, mustard oil which is rich in unsaturated fatty acids blending of vegetable oils is gaining popularity in the oil sector to widely used for both culinary and therapeutic purposes. Oil with satisfy consumer demands. Rice Bran Oil (RBO), a by-product of higher unsaturation are highly prone to oxidation [4]. Basically the rice milling industry contains unique antioxidant compound fatty acid composition of the oils is the main factor responsible for Copyright@ Mst Sarmina Yeasmin | Biomed J Sci & Tech Res | BJSTR. MS.ID.005620. 27167 Volume 34- Issue 5 DOI: 10.26717/BJSTR.2021.34.005620 oxidation and also for the formation of undesirable compounds according to the AOAC and AOCS Official Methods [12,13]. the production of a blending oil for edible purposes because of viscometer. They measure viscosity by sensing the torque required or flavours [5,6]. However, there is global increasing demand for Viscosity of all oils were determined by Fungilab digital Rotational improvedBlending fatty is acidsone of and the nutritional methods used benefits. to modify oils and fats for Determinationto rotate a spindle ofat constantFatty Acid speed Composition while immersed in fluid. wide range of applications. Evidences suggest that no single oil Preparation of Fatty Acid Methyl Ester: 200 mg of oil sample can provide the recommended dietary fatty acid ratio. According was taken in a 10 mL Pyrex test tube. 3.5 mL 0.5M Sodium methoxide to WHO and other health agencies, nutritionally superior oil must was added to the test tube and heated using burner until removing the bubbles. Then, 1.5 mL n-hexane was added to the mixture and The objective of the present investigation was to design an oil fall in the ratio of 1:1-3:1; SFA, MUFA and PUFA respectively [7]. mixing by vortex mixture. Then around 5 mL deionized water was blend which may provide an ideal recommended fatty acid ratio added to test tube slowly and waited for settle down. Organic upper regarding nutrition. layer was taken into the GC vial for GCMS analysis. Materials and Methods GCMS Condition: The gas chromatographic analyses of the Materials oils were performed on SHIMADZU GC-2010. GC was equipped Mustard seeds were procured from local market then fresh with auto-sampler (AOC- 20s), auto-injector (AOC-20i) and SH and healthy mustard seeds were stored for oil extraction. Freshly milled rice bran, outer layer of the grain was collected from rice Rxi 5MS Sill capillary column with 30m×0.25mm×0.25 µm film. milling industry, Rajshahi, Bangladesh and immediately processed oven temperature of GC was 1400C for 10.0 min, then increased at Helium was used as carrier gas at flow rate 2.0 mL/min. Initial after milling for stabilization. Primarily sieved with 100 mesh size to separate husk and broken rice grain. All solvents and chemicals temperature was 250 0C and injection volume was 1.0 µL with 75:1 70C/min to a final temperature of 250 0C for 10.0 min. The injector used were analytical, HPLC and GC grade. Fatty acids standards split ratio. Solvent cut time was 3.40 min and total run time was were purchased from Sigma-Aldrich. 35.71 min. The detector used was SHIMADZU GCMS-QP-2020 and temperature was 2550C. Extraction of Oils Rice bran oil was extracted by solvent extraction method. The Results and Discussion extraction process was conducted in the laboratory by Soxhlet Moisture is the amount of water present in edible oils. Moisture apparatus for 6 hours using n-hexane as extracted solvent. The should be as low as possible. The maximum allowed moisture ratio of rice bran:hexane used was 1:3 (w/v). n-hexane has been content in edible oils is 0.2% [14]. Previous studies have shown selected because the solvent has better effect over other polar that fungus species such as Aspergillus niger and Mucor species survive and reproduce when the moisture content value is higher oil was recovered by the evaporation of the solvent under reduced than 0.2% / [15]. Moisture content of all oils and their blends were solvents like alcohol, ketone, aldehyde ether and ester etc. [8]. The pressure using a Rotary vacuum evaporator and the percentage of determined presented in Table 1. All values were very low than oil content was calculated. Mustard oil was extracted by automated single oils and their blends were determined presented in Table 1. specified limit. Density, specific gravity and refractive index of all oil expelling machine. The refining process of the oils was carried Preparationout using the method of Blends as described by [9] with some modification. Results were within the WHO/FAO permissible limits. Viscosity of it can be observed from Table 1 that viscosity decreased in the oil is a measure of the oils resistance to flow. In the present study, Rice bran oil and mustard seed oil were taken in the ratio of blended oils than single vegetable oils, this is because blending 90:10, 70:30 and 60:40 respectively. Each blend was placed in 250 oil with different ratios decreases saturation as well as increases rpm for 15 min. Then the single oils and all blended oils were stored other researcher since oil viscosity depends on molecular structure ml beakers and were mixed by using a mechanical stirrer at 180 unsaturation fatty acids of oil. This phenomenon also verified by at room temperature in air tight glass bottle for physico-chemical and decreases with unsaturation of fatty acids [7]. It may due to and GCMS analyses. double bonds that make bonding more rigid and rotation between Analysis of Physicochemical Properties c-c bonds become more strenuous. Physical properties such as moisture content, refractive index, important quality parameters of edible oil. The results showed The Free Fatty Acid (FFA) and Acid Value (AV) are most were determined by the method described by Garba, et al. [10] and in Table 1 that the FFA value of all oils were very low and the density, specific gravity and viscosity of the single and blended oils mg KOH/g) [16]. All oils were of good quality because of the low AOAC [11].