Semi-Annual Report for the Time Period
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Comprehensive Mutagenesis of the Fims Promoter Regulatory Switch Reveals Novel Regulation of Type 1 Pili in Uropathogenic Escherichia Coli
Comprehensive mutagenesis of the fimS promoter regulatory switch reveals novel regulation of type 1 pili in uropathogenic Escherichia coli Huibin Zhanga, Teodorus T. Susantob, Yue Wanb, and Swaine L. Chena,c,1 aInfectious Diseases Group, Genome Institute of Singapore, Singapore 138672; bStem Cell and Development, Genome Institute of Singapore, Singapore 138672; and cDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119074 Edited by Roy Curtiss III, University of Florida, Gainesville, FL, and approved March 7, 2016 (received for review December 6, 2015) Type 1 pili (T1P) are major virulence factors for uropathogenic (11). These regulators act through a variety of mechanisms, such Escherichia coli (UPEC), which cause both acute and recurrent uri- as DNA structure and supercoiling (20–22), transcription ter- nary tract infections. T1P expression therefore is of direct relevance mination and RNA stability (23, 24), or dual effects on both for disease. T1P are phase variable (both piliated and nonpiliated phase variation and fimA transcription [such as through cAMP bacteria exist in a clonal population) and are controlled by an in- receptor protein (CRP), integration host factor (IHF), or growth vertible DNA switch (fimS), which contains the promoter for the fim phase] (18, 20, 25). operon encoding T1P. Inversion of fimS is stochastic but may be In vitro, the recombinases bind to sites flanking and over- biased by environmental conditions and other signals that ulti- lapping fimS inverted repeats (IRs) (26). On plasmid substrates, mately converge at fimS itself. Previous studies of fimS sequences FimB mediates switching in both directions, whereas FimE important for T1P phase variation have focused on laboratory-adapted performs only ON-to-OFF switching (27). -
Heraldic Terms
HERALDIC TERMS The following terms, and their definitions, are used in heraldry. Some terms and practices were used in period real-world heraldry only. Some terms and practices are used in modern real-world heraldry only. Other terms and practices are used in SCA heraldry only. Most are used in both real-world and SCA heraldry. All are presented here as an aid to heraldic research and education. A LA CUISSE, A LA QUISE - at the thigh ABAISED, ABAISSÉ, ABASED - a charge or element depicted lower than its normal position ABATEMENTS - marks of disgrace placed on the shield of an offender of the law. There are extreme few records of such being employed, and then only noted in rolls. (As who would display their device if it had an abatement on it?) ABISME - a minor charge in the center of the shield drawn smaller than usual ABOUTÉ - end to end ABOVE - an ambiguous term which should be avoided in blazon. Generally, two charges one of which is above the other on the field can be blazoned better as "in pale an X and a Y" or "an A and in chief a B". See atop, ensigned. ABYSS - a minor charge in the center of the shield drawn smaller than usual ACCOLLÉ - (1) two shields side-by-side, sometimes united by their bottom tips overlapping or being connected to each other by their sides; (2) an animal with a crown, collar or other item around its neck; (3) keys, weapons or other implements placed saltirewise behind the shield in a heraldic display. -
The History of Florida's State Flag the History of Florida's State Flag Robert M
Nova Law Review Volume 18, Issue 2 1994 Article 11 The History of Florida’s State Flag Robert M. Jarvis∗ ∗ Copyright c 1994 by the authors. Nova Law Review is produced by The Berkeley Electronic Press (bepress). https://nsuworks.nova.edu/nlr Jarvis: The History of Florida's State Flag The History of Florida's State Flag Robert M. Jarvis* TABLE OF CONTENTS I. INTRODUCTION ........ .................. 1037 II. EUROPEAN DISCOVERY AND CONQUEST ........... 1038 III. AMERICAN ACQUISITION AND STATEHOOD ......... 1045 IV. THE CIVIL WAR .......................... 1051 V. RECONSTRUCTION AND THE END OF THE NINETEENTH CENTURY ..................... 1056 VI. THE TWENTIETH CENTURY ................... 1059 VII. CONCLUSION ............................ 1063 I. INTRODUCTION The Florida Constitution requires the state to have an official flag, and places responsibility for its design on the State Legislature.' Prior to 1900, a number of different flags served as the state's banner. Since 1900, however, the flag has consisted of a white field,2 a red saltire,3 and the * Professor of Law, Nova University. B.A., Northwestern University; J.D., University of Pennsylvania; LL.M., New York University. 1. "The design of the great seal and flag of the state shall be prescribed by law." FLA. CONST. art. If, § 4. Although the constitution mentions only a seal and a flag, the Florida Legislature has designated many other state symbols, including: a state flower (the orange blossom - adopted in 1909); bird (mockingbird - 1927); song ("Old Folks Home" - 1935); tree (sabal palm - 1.953); beverage (orange juice - 1967); shell (horse conch - 1969); gem (moonstone - 1970); marine mammal (manatee - 1975); saltwater mammal (dolphin - 1975); freshwater fish (largemouth bass - 1975); saltwater fish (Atlantic sailfish - 1975); stone (agatized coral - 1979); reptile (alligator - 1987); animal (panther - 1982); soil (Mayakka Fine Sand - 1989); and wildflower (coreopsis - 1991). -
Flags of the World
ATHELSTANEFORD A SOME WELL KNOWN FLAGS Birthplace of Scotland’s Flag The name Japan means “The Land Canada, prior to 1965 used the of the Rising Sun” and this is British Red Ensign with the represented in the flag. The redness Canadian arms, though this was of the disc denotes passion and unpopular with the French sincerity and the whiteness Canadians. The country’s new flag represents honesty and purity. breaks all previous links. The maple leaf is the Another of the most famous flags Flags of the World traditional emblem of Canada, the white represents in the world is the flag of France, The foremost property of flags is that each one the vast snowy areas in the north, and the two red stripes which dates back to the represent the Pacific and Atlantic Oceans. immediately identifies a particular nation or territory, revolution of 1789. The tricolour, The flag of the United States of America, the ‘Stars and comprising three vertical stripes, without the need for explanation. The colours, Stripes’, is one of the most recognisable flags is said to represent liberty, shapes, sizes and devices of each flag are often in the world. It was first adopted in 1777 equality and fraternity - the basis of the republican ideal. linked to the political evolution of a country, and during the War of Independence. The flag of Germany, as with many European Union United Nations The stars on the blue canton incorporate heraldic codes or strongly held ideals. European flags, is based on three represent the 50 states, and the horizontal stripes. -
Heraldic Achievement of MOST REVEREND NELSON J
Heraldic Achievement of MOST REVEREND NELSON J. PEREZ Tenth Archbishop of Philadelphia Per pale: dexter, argent on a pile azure a mullet in chief of the field, overall on a fess sable three plates each charged with a cross throughout gules; sinister, per fess azure and chevronny inverted azure and Or, in chief a Star of Bethlehem argent and in base a mound Or, over all on a fess sable fimbriated argent, a Paschal Lamb reguardant, carrying in the dexter forelimb a palm branch Or and a banner argent charged with a Cross gules In designing the shield — the central element in what is formally called the heraldic achievement — an archbishop has an opportunity to depict symbolically various aspects of his own life and heritage, and to highlight aspects of Catholic faith and devotion that are important to him. The formal description of a coat of arms, known as the blazon, uses a technical language, derived from medieval French and English terms, which allows the appearance and position of each element in the achievement to be recorded precisely. An archbishop shows his commitment to the flock he shepherds by combining his personal coat of arms with that of the archdiocese, in a technique known as impaling. The shield is divided in half along the pale or central vertical line. The arms of the archdiocese appear on the dexter side — that is, on the side of the shield to the viewer’s left, which would cover the right side (in Latin, dextera) of the person carrying the shield. The arms of the archbishop are on the sinister side — the bearer’s left, the viewer’s right. -
Armorial Rules for Submission of the S.C.A. College of Arms Illustrated by Coblaith Mhuimhneach
Armorial Rules for Submission of the S.C.A. College of Arms Illustrated by Coblaith Mhuimhneach My son, Áed, is fascinated (some would say obsessed) with heraldry. When he was eight years old, he reached a level in his understanding of heraldry as practiced in the S.C.A. at which the next logical step was to study the armory-related sections of the Rules for Submission. I read through them, and found visualizing the example devices and comparisons troublesome, so I decided to spare him the effort. The images in this document were the eventual result. I would like to thank Daniel de Lincoln, Jaelle of Armida, Meradudd Cethin, and Julianna de Luna for taking the time to share their heraldic expertise with me, thus much improving the quality of my depictions. There were some portions of the rules that I did not believe my son had, at that time, the discrimination and general background knowledge to comprehend even with illustrations. I did not create images for those portions. If you would like to see some, contact me. Sufficient interest would probably spur me to make them. In the mean time, the text of the un- illustrated portions are included here for the user’s convenience. You can find the current, definitive version of the RfS on the S.C.A. College of Arms’ website, at http://heraldry.sca.org. The graphics in this document are under my copyright, as of 2008. You may distribute them as you wish, so long as you credit me as their creator and do not sell them at a profit. -
14 Flags Over Oklahoma Trunk Contents
14 Flags Trunk Contents Te Over Oklahoma Hands-on Items 14 Flags – This trunk includes 14 full-size cloth flags that represent the 14 flags over Oklahoma. You may display these in the classroom and let the students pick them up and examine them. Royal Standard of Spain, circa 1513 – According to the Oklahoma Historical Society, this was the first flag to fly over Oklahoma. A Spanish explorer named Coronado brought it to Oklahoma in 1541. The red- and-white quartered flag has a golden castle on the red and a red lion on the white. The castle and lion represented royal houses Castile and Leon, from which the King of Spain descended. The Great Union Flag of Great Britain – This flag flew over Oklahoma beginning in 1663 when King Charles II gave his friends a wide strip of country called Carolina, which stretched from the Atlantic to the Pacific. 1 14 Flags Trunk Contents Te French Flag – Bernard de la Harpe brought this flag to the region in 1719 when he visited an Indian village on the Arkansas River near present-day Haskell in Muskogee County. However, the French claims on this area go back to 1682 when La Salle claimed all the country drained by the Mississippi River and its branches in the name of France. Cross of Burgundy, circa 1506 – In 1763 France gave all the country west of the Mississippi to Spain at the end of the Seven Years' War via the Treaty of Paris. Both French and Spanish explorers had established trading posts in the area. -
Heraldry in Game of Thrones
genealogy Article The Shields that Guard the Realms of Men: Heraldry in Game of Thrones Mat Hardy School of Humanities & Social Sciences, Faculty of Arts & Education, Deakin University, Burwood 3125, Australia; [email protected] Received: 12 October 2018; Accepted: 6 November 2018; Published: 12 November 2018 Abstract: The vast popularity of the Game of Thrones franchise has drawn a new and diverse audience to the fantasy genre. Within the pseudo-medieval world created by G.R.R. Martin, a great deal of detail has gone into establishing coats of arms for the characters and families that are depicted. These arms fulfill an extremely important role, both within the arc of the story and as part of the marketing collateral of this very successful series. This article examines the role of arms in the Game of Thrones universe and explores how the heraldic system transcends the usual genealogical display and functions more as a type of familial branding. An exploration of some of the practices and idiosyncrasies of heraldry in the franchise shows that whilst Martin sets his foundation firmly in the traditional, he then extends this into the fanciful; in much the same manner as he does with other faux-historical aspects of his work. This study is valuable because Game of Thrones has brought heraldry from being a niche interest to something that is now consumed by a global audience of hundreds of millions of people. Several of the fantasy blazons in the series are now arguably the most recognisable coats of arms in history. Keywords: Game of Thrones; A Song of Ice and Fire; heraldry; blazonry; fantasy; G.R.R. -
Ing Items Have Been Registered
ACCEPTANCES Page 1 of 27 April 2012 LoAR THE FOLLOWING ITEMS HAVE BEEN REGISTERED: ÆTHELMEARC Faelan mac Colmain. Name. Ylaire Saint Claire. Name change from Ylaire le Enguigniur. Commenters questioned whether Saint Claire needed to be changed to Sainte Claire. However, Green Staff was able to find medieval examples of bynames using the submitted spelling. Therefore, this can be registered as submitted. The submitter’s previous name, Ylaire le Enguigniur, is released. AN TIR Andrew of Dragon’s Mist. Reblazon of device. Sable, between the horns of a crescent argent a wolf’s head erased Or and on a chief argent three mullets sable. Blazoned when registered in October 1994 as Sable, a wolf’s head erased Or between the horns of a crescent and on a chief argent three mullets sable, the crescent is the primary charge, with the wolf’s head a secondary charge. Anne Midwinter. Name. Cerridwen Maelwedd. Reblazon of device. Vert, between the horns of a crescent argent a sea-lion statant Or, a chief embattled ermine. Blazoned when registered in January 1995 as Vert, a sea lion statant Or within the horns of a crescent argent and a chief embattled ermine, the crescent is the primary charge and the sea-lion is a secondary charge. Dýrfinna þeysir. Name. Edmund Halliday. Device. Or, semy of trefoil knots inverted azure, a crane close contourny sable within an orle vert. Electra de Flora. Reblazon of device. Per bend purpure and gules, a crescent bendwise sinister and a cinquefoil and between the horns of the crescent a mullet argent. -
Habitat Characterization and Sea Scallop Resource Enhancement Study in a Proposed Habitat Research Area
Habitat Characterization and Sea Scallop Resource Enhancement Study in a Proposed Habitat Research Area Final Report Prepared for the 2013 Sea Scallop Research Set-Aside May 2014 Submitted by Coonamessett Farm Foundation, Inc In Collaboration with Kevin Stokesbury, Susan Inglis, Changsheng Chen - SMAST Coonamessett Farm Foundation, Inc 277 Hatchville Road East Falmouth, Massachusetts, USA 02536 508-564-5516 FAX 508-564-5073 [email protected] NOAA Grant Number: NA13NMF4540009 A. Grantee: Coonamessett Farm Foundation, Inc B. Project Title: Habitat Characterization and Sea Scallop Resource Enhancement Study in a Proposed Habitat Research Area C. Amount of Grant: $201,609.00 D. Award Period: 03/01/2013 - 02/28/2014 E. Reporting Period: 03/01/2013 - 02/28/2014 Project Summary: Scallop seed from a natural seed bed was harvested in the Great South Channel on Georges Bank and transplanted to an experimental seed bed in Closed Area I (CAI) which has been identified as a potential Dedicated Habitat Research Area (DHRA). The encompassing goal of this project was to demonstrate the feasibility of a seeding program to enhance and stabilize scallop recruitment on Georges Bank while documenting the factors that affect seed survival. The success of the transplanting experiment was evaluated with optical surveys using drop cameras operated by the University of Massachusetts, Dartmouth School for Marine Science and Technology (SMAST) and an off-bottom towed benthic sled (HabCam II operated by Arnie’s Fisheries). The goal was to use images to evaluate scallop survival, scallop growth, and dispersal of scallop seed, as well as changes in population density of scallops and predators after the seeding operation. -
Heraldry in Macedonia with Special Regard to the People's/Socialist
genealogy Article Heraldry in Macedonia with Special Regard to the People’s/Socialist Republic of Macedonia until 1991 Jovan Jonovski Macedonian Heraldic Society, 1000 Skopje, North Macedonia; [email protected] or [email protected]; Tel.: +389-70-252-989 Abstract: Every European region and country has some specific heraldry. In this paper, we will consider heraldry in the People’s/Socialist Republic of Macedonia, understood by the multitude of coats of arms, and armorial knowledge and art. Due to historical, as well as geographical factors, there is only a small number of coats of arms and a developing knowledge of art, which make this paper’s aim feasible. This paper covers the earliest preserved heraldic motifs and coats of arms found in Macedonia, as well as the attributed arms in European culture and armorials of Macedonia, the кing of Macedonia, and Alexander the Great of Macedonia. It also covers the land arms of Macedonia from the so-called Illyrian Heraldry, as well as the state and municipal heraldry of P/SR Macedonia. The paper covers the development of heraldry as both a discipline and science, and the development of heraldic thought in SR Macedonia until its independence in 1991. Keywords: heraldry of Macedonia; coats of arms of Macedonia; socialist heraldry; Macedonian municipal heraldry 1. Introduction Macedonia, as a region, is situated on the south of Balkan Peninsula in Southeast Citation: Jonovski, Jovan. 2021. Europe. The traditional boundaries of the geographical region of Macedonia are the lower Heraldry in Macedonia with Special Néstos (Mesta in Bulgaria) River and the Rhodope Mountains to the east; the Skopska Crna Regard to the People’s/Socialist Gora and Shar mountains, bordering Southern Serbia, in the north; the Korab range and Republic of Macedonia until 1991. -
HERALDRY MANUAL GUIDE: RULES for REGISTRATON and ADMINISTRATION
The Adrian Empire, Inc. HERALDRY MANUAL GUIDE: RULES FOR REGISTRATON and ADMINISTRATION This document is presented as a GUIDE. It is the Heraldry Manual: Rules for Registration and Administration as adopted October 1999 and amended November 2001. It also contains rulings, policies, and clarifications as published by the Imperial College of Arms since November 2001. This is NOT an official document of the Adrian Empire (it has not been adopted for use by any body within the Adrian Empire). It is a REFERENCE tool so that the College of Arms may consolidate its rules and regulations. ~Maedb Hawkins, Imperial Office of Publishing. As adopted October 1999 DRAFTamended November 2001 DRAFT AMENDMENTS ADDED MAY 2004 © 2004 The Adrian Empire Inc., all rights reserved. Anyone is welcome to point out any error or omission that they may find. Imperial Sovereign of Arms [email protected] Empress [email protected] Emperor [email protected] Page 2 of 35 DRAFT Heraldry Manual Guide as amended May 2004 TABLE OF CONTENTS Preface ................................................................................................................................................................5 I. The Rule of Tincture .......................................................................................................................................5 A. Simple Ordinaries.............................................................................................................................5 B. Field Divisions..................................................................................................................................5