DOCSLIB.ORG

GRAS Notice (GRN) No. 761, Esterified Propoxylated Glycerol

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
GRAS Notice (GRN) No. 761, Esterified Propoxylated Glycerol GRAS Notice (GRN) No. 761 https://www.fda.gov/food/generally-recognized-safe-gras/gras-notice-inventory February 20, 2018 Office ofFood Additive Safety HFS-200 Center for foodSafetyand Applied Nutrition food and Drug Administratioo 5001 Campus Drive College Park, MD, 20740 Dear Sir or Madam: Accompanying this letterisa notice pursuantto regulationsofthe Food and Orug Administration found at 21 CFR Part 170 settingforth the basis forthe conclusion reached by the submitter, Choco Finesse, LLC, that esterified propoxylated glycerol (EPG) is generally recognized as safe underthe intended conditions ofuse described in the notice. The notice is contained in a binder. In addition, we include a CD that contains a complete copyofthe notice. I hereby certify thatthe electronicfilescontained onthe flash drivewere scanned forviruses priorto submission, and thuscertified as beingvirus-free using Symantec Endpoint Protection. Sine I,/ .. (b) (6) '---'--------..... David Rowe President Phone: 317-694-3601 Email: drowe@chocofi nesse .com Ft -1 2 2 2018 z., ,..,.. ,. ; FOOD ADDITivE SAFETY GRAS NOTICE FOR ESTERIFIED PROPOXYLATED GLVCEROL {EPG) FOR USE IN SELECT COMMERCIAL FRYING APPLICATIONS Prepared for: Office of Food Additive Safety (HFS-200} Center for Food Safety and Applied Nutrition Food and Drug Administration 5001 Campus Drive College Park, MD 20740 Prepared by: Choco Finesse, LLC 5019 N. Meridian Street Indianapolis, Indiana 46208 February 20, 2018 rR1~~~~~~[Q) FEB 2 2 2018 OFFICE OF FOOD ADDmVE SAFETY GRAS Notice for Esterified Propoxylated Glycerol (EPG) for Use in Select Commercial Frying Applications TABLE OF CONTENTS Part 1. §170.225 Signed Statements and Certification ..................................................................................... 4 1.1 Name and Address of Notifier .................................................................................................. 4 1.2 Common Name of Notified Substance ..................................................................................... 4 1.3 Conditions of Use ...................................................................................................................... 5 1.4 Basis for GRAS ........................................................................................................................... 6 1.5 Availability of Information ........................................................................................................ 6 1.6 Freedom of Information Act, 5 U.S.C. 552 ................................................................................ 6 Part 2. §170.230 Identity, Method of Manufacture, Specifications, and Physical or Technical Effect ............. 7 2.1 Description ................................................................................................................................ 7 2.2 Manufacturing .......................................................................................................................... 7 2.2.1 Overview ...................................................................................................................... 7 2.2.2 Raw Material Specifications......................................................................................... 8 2.2.3 Detailed Description of the Manufacturing Process .................................................... 9 2.3 Product Specifications ............................................................................................................ 10 2.3.1 Proposed Chemical Specifications ............................................................................. 11 2.3.2 Microbiological Specifications ................................................................................... 14 2.4 Product Analysis ...................................................................................................................... 14 2.4.1 Chemical Analysis ...................................................................................................... 14 2.4.2 Microbiological Analysis ............................................................................................ 16 2.4.3 Additional Analytical Information .............................................................................. 16 Part 3. §170.235 Dietary Exposure .................................................................................................................. 19 3.1 History of Use in Food ............................................................................................................. 19 3.2 Intended Use of EPG and Levels of Use in Foods ................................................................... 19 3.3 Estimated Consumption of EPG from All Intended Conditions of Use in Food ...................... 25 3.3.1 Dietary Intake Survey ................................................................................................. 25 3.3.2 Estimated Consumption of EPG from Proposed Food-Uses ...................................... 26 3.3.3 Cumulative Intakes .................................................................................................... 28 Part 4. §170.240 Self-Limiting Levels of Use ................................................................................................... 28 Part 5. §170.245 Experience Based on Common Use in Food Before 1958 ................................................... 28 Part 6. §170.250 Narrative and Safety Information ........................................................................................ 29 6.1 Introduction ............................................................................................................................ 29 6.2 Literature Search..................................................................................................................... 30 6.3 Absorption, Distribution, Metabolism, and Excretion ............................................................ 30 6.3.1 ADME Studies ............................................................................................................ 31 6.4 Pivotal Toxicology Studies ...................................................................................................... 37 6.4.1 Repeat-Dose Toxicity ................................................................................................. 37 6.4.2 Reproductive and Developmental Toxicity Studies ................................................... 39 Choco Finesse, LLC 1 February 20, 2018 6.4.3 Genotoxicity Testing of Esterified Propoxylated Glycerol (EPG) (Bechtel, 2014) .......................................................................................................................... 40 6.5 Corroborative Evidence of Safety ........................................................................................... 45 6.5.1 Repeat-Dose Toxicity ................................................................................................. 45 6.5.2 Reproductive and Developmental Toxicity ................................................................ 51 6.5.3 Irritation and Sensitization Studies ............................................................................ 53 6.6 Clinical Safety .......................................................................................................................... 55 6.6.1 Tolerance Studies with H-EPG-05 HR/SO (9:1) .......................................................... 58 6.6.2 Tolerance Studies with EPG-05 HR/ST 45:55 (Softer Version on EPG) ...................... 58 6.7 Information Potentially Inconsistent with GRAS .................................................................... 66 6.7.1 Gastrointestinal Discomfort ....................................................................................... 66 6.7.2 Effect on Nutrient (Fat-Soluble Vitamin) Status ........................................................ 66 6.7.3 Effect on β-Carotene Levels ....................................................................................... 70 6.7.4 Effect on Serum HDL Levels ....................................................................................... 70 6.7.5 Effect on Serum Transaminase Levels ....................................................................... 73 6.8 Expert Panel Evaluation .......................................................................................................... 77 6.9 Conclusions ............................................................................................................................. 78 Part 7. §170.255 List of Supporting Data and Information ............................................................................. 79 List of Appendices Appendix 1 Certificate of Analysis – Propylene Oxide Appendix 2 Tocopherol Safety Summary Appendix 3 Estimated Daily Intake of Esterified Propoxylated Glycerol (EPG) by the U.S. Population from intended Frying Applications (2013-2014 NHANES) Appendix 4 Expert Panel Consensus Statement List of Figures and Tables Figure 2.2-1 Schematic Overview of the Manufacturing Process for EPG.................................................. 8 Figure 6.6.2.3-1 Mean Retinol Levels Over Time ............................................................................................ 62 Figure 6.6.2.3-2 Mean α-Tocopherol Levels Over Time ................................................................................. 63 Figure 6.6.2.3-3 Mean Circulating 25-OH D2 (Ergocalciferol) Levels Over Time ............................................ 64 Figure 6.6.2.3-4
Load more

Recommended publications
  • Retention Indices for Frequently Reported Compounds of Plant Essential Oils
    Retention Indices for Frequently Reported Compounds of Plant Essential Oils V. I. Babushok,a) P. J. Linstrom, and I. G. Zenkevichb) National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA (Received 1 August 2011; accepted 27 September 2011; published online 29 November 2011) Gas chromatographic retention indices were evaluated for 505 frequently reported plant essential oil components using a large retention index database. Retention data are presented for three types of commonly used stationary phases: dimethyl silicone (nonpolar), dimethyl sili- cone with 5% phenyl groups (slightly polar), and polyethylene glycol (polar) stationary phases. The evaluations are based on the treatment of multiple measurements with the number of data records ranging from about 5 to 800 per compound. Data analysis was limited to temperature programmed conditions. The data reported include the average and median values of retention index with standard deviations and confidence intervals. VC 2011 by the U.S. Secretary of Commerce on behalf of the United States. All rights reserved. [doi:10.1063/1.3653552] Key words: essential oils; gas chromatography; Kova´ts indices; linear indices; retention indices; identification; flavor; olfaction. CONTENTS 1. Introduction The practical applications of plant essential oils are very 1. Introduction................................ 1 diverse. They are used for the production of food, drugs, per- fumes, aromatherapy, and many other applications.1–4 The 2. Retention Indices ........................... 2 need for identification of essential oil components ranges 3. Retention Data Presentation and Discussion . 2 from product quality control to basic research. The identifi- 4. Summary.................................. 45 cation of unknown compounds remains a complex problem, in spite of great progress made in analytical techniques over 5.
    [Show full text]
  • VITAMIN K1 | C31H46O2 - Pubchem
    VITAMIN K1 | C31H46O2 - PubChem https://pubchem.ncbi.nlm.nih.gov/compound/Phylloquinone#secti... NIH U.S. National Library of Medicine National Center for Biotechnology Information OPEN CHEMISTRY Search Compounds ! DATABASE VITAMIN K1 " Cite this Record # $ % & ' ( STRUCTURE VENDORS DRUG INFO PHARMACOLOGY LITERATURE PATENTS BIOACTIVITIES PubChem CID: 5284607 VITAMIN K1; Phytonadione; Phylloquinone; 84-80-0; Phytylmenadione; Chemical Names: Phyllochinon More... Molecular Formula: C31H46O2 Molecular Weight: 450.707 g/mol InChI Key: MBWXNTAXLNYFJB-NKFFZRIASA-N Drug Indication Therapeutic Uses Clinical Trials FDA Orange Book Drug Information: FDA UNII Safety Summary: Laboratory Chemical Safety Summary (LCSS) VITAMIN K1 is a family of phylloquinones that contains a ring of 2-methyl-1,4-naphthoquinone and an isoprenoid side chain. Members of this group of vitamin K 1 have only one double bond on the proximal isoprene unit. Rich sources of vitamin K 1 include green plants, algae, and photosynthetic bacteria. Vitamin K1 has antihemorrhagic and prothrombogenic activity. " from MeSH Vitamin K is a family of fat-soluble compounds with a common chemical structure based on 2-methyl-1, 4-naphthoquinone " Metabolite Description from Human Metabolome Database (HMDB) PUBCHEM ) COMPOUND ) VITAMIN K1 Modify Date: 2018-01-06; Create Date: 2004-09-16 1 di 57 12/01/18, 11:36 VITAMIN K1 | C31H46O2 - PubChem https://pubchem.ncbi.nlm.nih.gov/compound/Phylloquinone#secti... * Contents 1 2D Structure 2 3D Conformer 3 Names and Identifiers + 4 Chemical and Physical Properties 5 Related Records 6 Chemical Vendors 7 Drug and Medication Information 8 Pharmacology and Biochemistry 9 Use and Manufacturing 10 Identification 11 Safety and Hazards 12 Toxicity 13 Literature 14 Patents 15 Biomolecular Interactions and Pathways 16 Biological Test Results 17 Classification 18 Information Sources 2 di 57 12/01/18, 11:36 VITAMIN K1 | C31H46O2 - PubChem https://pubchem.ncbi.nlm.nih.gov/compound/Phylloquinone#secti..
    [Show full text]
  • Peroxisomal Trans-2-Enoyl-Coa Reductase Is Involved in Phytol Degradation
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector FEBS Letters 580 (2006) 2092–2096 Peroxisomal trans-2-enoyl-CoA reductase is involved in phytol degradation J. Gloerich, J.P.N. Ruiter, D.M. van den Brink, R. Ofman, S. Ferdinandusse, R.J.A. Wanders* Laboratory Genetic Metabolic Diseases (F0-224), Departments of Clinical Chemistry and Pediatrics, Emma’s Children’s Hospital, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands Received 15 February 2006; accepted 4 March 2006 Available online 10 March 2006 Edited by Sandro Sonnino the peroxisomal acyl-CoA oxidases anymore. For further Abstract Phytol is a naturally occurring precursor of phytanic acid. The last step in the conversion of phytol to phytanoyl-CoA breakdown, the chain-shortened product is transported to is the reduction of phytenoyl-CoA mediated by an, as yet, the mitochondrion [3], where it is totally degraded to acetyl- unidentified enzyme. A candidate for this reaction is a previously CoA and propionyl-CoA units [4]. Besides breakdown of described peroxisomal trans-2-enoyl-CoA reductase (TER). To pristanic acid, peroxisomal b-oxidation is also involved in investigate this, human TER was expressed in E. coli as an the degradation of very long-chain fatty acids, long-chain MBP-fusion protein. The purified recombinant protein was dicarboxylic acids and bile acid intermediates [4]. shown to have high reductase activity towards trans-phytenoyl- Another metabolic process that recently has been shown to CoA, but not towards the peroxisomal b-oxidation intermediates occur, at least partly, in the peroxisome is the degradation of C24:1-CoA and pristenoyl-CoA.
    [Show full text]
  • Tetrahydrogeranylgeraniol, a Precursor of Phytol in The
    Tetrahydrogeranylgeraniol, a Precursor of Phytol in the Biosynthesis of Chlorophyll a — Localization of the Double Bonds Siegrid Schoch and Wolfram Schäfer Botanisches Institut der Universität München, München und Max-Planck-Institut für Biochemie, Martinsried Z. Naturforsch. 33 c, 408—412 (1978) ; received March 23, 1978 Dedicated to Prof. Dr. A. Butenandt on the Occasion of His 75. Birthday Avena sativa, Gramineae, Oats, Chlorophyll Biosynthesis, Phytol Pheophytins esterified with phytol and tetrahydrogeranylgeraniol are isolated from etiolated oat seedlings after short (1 min) exposure to light and a subsequent dark period of 15 to 20 min. After saponification of the pheophytins, a mixture of the alcohols was isolated. The structure of tetra­ hydrogeranylgeraniol was established as 3,7,11,15-tetramethyl-zl2’14 hexadecadiene-l-ol (3a). The implications for chlorophyll biosynthesis are discussed. Introduction The number of double bonds in the alcohols have been established from the molecular ions in their During our research on the last steps of chloro­ mass spectra [3, 4]. The heaviest alcohol is P (4), phyll biosynthesis, we could isolate pheophytins the alcohols THGG (3), DHGG (2) and GG (1) which are esterified with not only phytol (P), but are smaller by 2, 4 and 6 mass units respectively. also with tetrahydrogeranylgeraniol (THGG), dihy- To further evaluate this sequence it is necessary drogeranylgeraniol (DHGG) and geranylgeraniol to establish the order of hydrogenation of the three (GG) [1]. double bonds zl-6, A-10, A-\4> when transforming Based on these findings, a biosynthetic sequence GG to P. has been proposed, in which chlorophyllide a is first As the pigments were available only in small esterified to Chl^G • This pigment should then hy­ amounts (5 — 10nmol/g fresh weight), the alcohols drogenated successively to CMdiigg > CIiIthgg and were identified and the double bonds localized by finally to Chip [1], The activated alcohol substrate gc-ms technique combined with microchemical proce­ for the esterification is probably geranylgeraniol- dures.
    [Show full text]
  • Open Natural Products Research: Curation and Dissemination of Biological Occurrences of Chemical Structures Through Wikidata
    bioRxiv preprint doi: https://doi.org/10.1101/2021.02.28.433265; this version posted March 1, 2021. The copyright holder has placed this preprint (which was not certified by peer review) in the Public Domain. It is no longer restricted by copyright. Anyone can legally share, reuse, remix, or adapt this material for any purpose without crediting the original authors. Open Natural Products Research: Curation and Dissemination of Biological Occurrences of Chemical Structures through Wikidata Adriano Rutz1,2, Maria Sorokina3, Jakub Galgonek4, Daniel Mietchen5, Egon Willighagen6, James Graham7, Ralf Stephan8, Roderic Page9, Jiˇr´ıVondr´aˇsek4, Christoph Steinbeck3, Guido F. Pauli7, Jean-Luc Wolfender1,2, Jonathan Bisson7, and Pierre-Marie Allard1,2 1School of Pharmaceutical Sciences, University of Geneva, CMU - Rue Michel-Servet 1, CH-1211 Geneva 4, Switzerland 2Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU - Rue Michel-Servet 1, CH-1211 Geneva 4, Switzerland 3Institute for Inorganic and Analytical Chemistry, Friedrich-Schiller-University Jena, Lessingstr. 8, 07732 Jena, Germany 4Institute of Organic Chemistry and Biochemistry of the CAS, Flemingovo n´amˇest´ı2, 166 10, Prague 6, Czech Republic 5School of Data Science, University of Virginia, Dell 1 Building, Charlottesville, Virginia 22904, United States 6Dept of Bioinformatics-BiGCaT, NUTRIM, Maastricht University, Universiteitssingel 50, NL-6229 ER, Maastricht, The Netherlands 7Center for Natural Product Technologies, Program for Collaborative Research
    [Show full text]
  • Plastid Gene Expression and Plant Development Require a Plastidic Protein of the Mitochondrial Transcription Termination Factor Family
    Plastid gene expression and plant development require a plastidic protein of the mitochondrial transcription termination factor family Elena Babiychuka,b, Klaas Vandepoelea,b, Josef Wissingc, Miguel Garcia-Diazd, Riet De Ryckea,b, Hana Akbarie, Jérôme Joubèsf, Tom Beeckmana,b, Lothar Jänschc, Margrit Frentzene, Marc C. E. Van Montagub,1, and Sergei Kushnira,b,1 aDepartment of Plant Systems Biology, VIB, 9052 Ghent, Belgium; bDepartment of Plant Biotechnology and Genetics, Ghent University, 9052 Ghent, Belgium; cAbteilung Zellbiologie, Helmholtz-Zentrum für Infektionsforschung GmbH, 38124 Braunschweig, Germany; dPharmacological Sciences, Stony Brook University, Stony Brook, NY 11794-8651; eInstitut für Biologie I, Spezielle Botanik, Rheinisch-Westfälische Technische Hochschule Aachen, 52056 Aachen, Germany; and fUniversité Victor Ségalen Bordeaux 2, Laboratoire de Biogenèse Membranaire, Centre National de la Recherche Scientifique, 33076 Bordeaux Cedex, France Contributed by Marc C. E. Van Montagu, March 3, 2011 (sent for review December 6, 2010) Plastids are DNA-containing organelles unique to plant cells. In fluence transcription (8). mTERF3 acts as a specific repressor of Arabidopsis, one-third of the genes required for embryo devel- mammalian mtDNA transcription initiation in vivo (9). opment encode plastid-localized proteins. To help understand the Here, we show that 11 of 35 annotated Arabidopsis mTERFs role of plastids in embryogenesis and postembryonic develop- are targeted to plastids. Genetic complementation indicated that ment, we characterized proteins of the mitochondrial transcrip- early embryo arrest is a characteristic phenotype of mutation in tion termination factor (mTERF) family, which in animal models, mTERF/At4g02990, whereas in vitro cell culture and analysis of comprises DNA-binding regulators of mitochondrial transcription.
    [Show full text]
  • ACS Style Guide
    ➤ ➤ ➤ ➤ ➤ The ACS Style Guide ➤ ➤ ➤ ➤ ➤ THIRD EDITION The ACS Style Guide Effective Communication of Scientific Information Anne M. Coghill Lorrin R. Garson Editors AMERICAN CHEMICAL SOCIETY Washington, DC OXFORD UNIVERSITY PRESS New York Oxford 2006 Oxford University Press Oxford New York Athens Auckland Bangkok Bogotá Buenos Aires Calcutta Cape Town Chennai Dar es Salaam Delhi Florence Hong Kong Istanbul Karachi Kuala Lumpur Madrid Melbourne Mexico City Mumbai Nairobi Paris São Paulo Singapore Taipei Tokyo Toronto Warsaw and associated companies in Berlin Idaban Copyright © 2006 by the American Chemical Society, Washington, DC Developed and distributed in partnership by the American Chemical Society and Oxford University Press Published by Oxford University Press, Inc. 198 Madison Avenue, New York, NY 10016 Oxford is a registered trademark of Oxford University Press All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior permission of the American Chemical Society. Library of Congress Cataloging-in-Publication Data The ACS style guide : effective communication of scientific information.—3rd ed. / Anne M. Coghill [and] Lorrin R. Garson, editors. p. cm. Includes bibliographical references and index. ISBN-13: 978-0-8412-3999-9 (cloth : alk. paper) 1. Chemical literature—Authorship—Handbooks, manuals, etc. 2. Scientific literature— Authorship—Handbooks, manuals, etc. 3. English language—Style—Handbooks, manuals, etc. 4. Authorship—Style manuals. I. Coghill, Anne M. II. Garson, Lorrin R. III. American Chemical Society QD8.5.A25 2006 808'.06654—dc22 2006040668 1 3 5 7 9 8 6 4 2 Printed in the United States of America on acid-free paper ➤ ➤ ➤ ➤ ➤ Contents Foreword.
    [Show full text]
  • Peroxisomal Cofactor Transport
    biomolecules Review Peroxisomal Cofactor Transport Anastasija Plett, Lennart Charton and Nicole Linka * Institute of Plant Biochemistry and Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine University, Universitätsstrasse 1, 40,225 Düsseldorf, Germany; [email protected] (A.P.); [email protected] (L.C.) * Correspondence: [email protected]; Tel.: +49-(0)211-81-10412; Fax: +49-(0)211-81-13706 Received: 2 July 2020; Accepted: 7 August 2020; Published: 12 August 2020 Abstract: Peroxisomes are eukaryotic organelles that are essential for growth and development. They are highly metabolically active and house many biochemical reactions, including lipid metabolism and synthesis of signaling molecules. Most of these metabolic pathways are shared with other compartments, such as Endoplasmic reticulum (ER), mitochondria, and plastids. Peroxisomes, in common with all other cellular organelles are dependent on a wide range of cofactors, such as adenosine 50-triphosphate (ATP), Coenzyme A (CoA), and nicotinamide adenine dinucleotide (NAD). The availability of the peroxisomal cofactor pool controls peroxisome function. The levels of these cofactors available for peroxisomal metabolism is determined by the balance between synthesis, import, export, binding, and degradation. Since the final steps of cofactor synthesis are thought to be located in the cytosol, cofactors must be imported into peroxisomes. This review gives an overview about our current knowledge of the permeability of the peroxisomal membrane with the focus on ATP, CoA, and NAD. Several members of the mitochondrial carrier family are located in peroxisomes, catalyzing the transfer of these organic cofactors across the peroxisomal membrane. Most of the functions of these peroxisomal cofactor transporters are known from studies in yeast, humans, and plants.
    [Show full text]
  • Safety Assessment of Squalane and Squalene As Used in Cosmetics
    Safety Assessment of Squalane and Squalene as Used in Cosmetics Status: Re-Review for Panel Review Release Date: March 15, 2019 Panel Meeting Date: April 8-9, 2019 The 2019 Cosmetic Ingredient Review Expert Panel members are: Chair, Wilma F. Bergfeld, M.D., F.A.C.P.; Donald V. Belsito, M.D.; Ronald A. Hill, Ph.D.; Curtis D. Klaassen, Ph.D.; Daniel C. Liebler, Ph.D.; James G. Marks, Jr., M.D., Ronald C. Shank, Ph.D.; Thomas J. Slaga, Ph.D.; and Paul W. Snyder, D.V.M., Ph.D. The CIR Executive Director is Bart Heldreth, Ph.D. This safety assessment was prepared by Monice M. Fiume, Senior Director. © Cosmetic Ingredient Review 1620 L Street, NW, Suite 1200 ♢ Washington, DC 20036-4702 ♢ ph 202.331.0651 ♢ fax 202.331.0088 ♢ [email protected] Distributed for Comment Only -- Do Not Cite or Quote Commitment & Credibility since 1976 Memorandum To: CIR Expert Panel Members and Liaisons From: Monice M. Fiume MMF Senior Director Date: March 15, 2019 Subject: Re-Review of the Safety Assessment of Squalane and Squalene The CIR Expert Panel first reviewed the safety of Squalane and Squalene in 1982. The Panel concluded that “both Squalane and Squalene are safe as cosmetic ingredients in the present practices of use and concentration,” as described in that report (identified as squal042019orig in the pdf). In 2001, after considering new studies and updated use data on these two ingredients, the Panel determined to not re-open the safety assessment (squal042019RR1sum). The minutes from the Panel deliberations of that re-review are included (squal042019min_RR1).
    [Show full text]
  • Feodor Lynen
    FEODOR LYNEN The pathway from "activated acetic acid" to the terpenes and fatty acids Nobel Lecture, December 11, 1964 The extreme distinction conferred by the Royal Caroline Institute on the work of Konrad Bloch and myself "On the Mechanism and Regulation of the Cholesterol and Fatty Acid Metabolism" places on me the honoured duty of presenting a report on my investigations to this assembly. My first contact with dynamic biochemistry in 1937 occurred at an exceed- ingly propitious time. The remarkable investigations on the enzyme chain of respiration, on the oxygen-transferring haemin enzyme of respiration, the cytochromes, the yellow enzymes, and the pyridine proteins had thrown the first rays of light on the chemical processes underlying the mystery of biologi- cal catalysis, which had been recognised by your famous countryman Jöns Jakob Berzelius. Vitamin B2, which is essential to the nourishment of man and of animals, had been recognised by Hugo Theorell in the form of the phosphate ester as the active group of an important class of enzymes, and the fermentation processes that are necessary for Pasteur’s "life without oxygen" had been elucidated as the result of a sequence of reactions centered around "hydrogen shift" and "phosphate shift" with adenosine triphosphate as the phosphate-transferring coenzyme. However, 1,3-diphosphoglyceric acid, the key substance to an understanding of the chemical relation between oxi- dation and phosphorylation, still lay in the depths of the unknown. Never- theless, Otto Warburg was on its trail in the course of his investigations on the fermentation enzymes, and he was able to present it to the world in 1939.
    [Show full text]
  • ABSTRACT COOK, MALLORY CAROLINE. Phytanic Acid
    ABSTRACT COOK, MALLORY CAROLINE. Phytanic Acid: Enrichment in the Rumen and Implications for Human Health. (Under the direction of Vivek Fellner). Phytanic acid is a known peroxisome proliferator-activated receptor-α (PPAR-α) and retinoid X Receptor (RXR) agonist. RXR and PPAR-α aid in cell and lipid metabolism, being major substrate regulators skewing metabolism from glucose to lipid metabolism. In studies, rats that had higher circulating phytanic acid became hyperphagic without subsequent weight gain. Patients suffering Refsum’s disease, a condition causing elevated levels of phytanic acid in the body, also lost weight; yet another factor indicating beneficial fat lipolysis with increased dietary phytanic acid. Previous work has shown that ruminant diets with increased levels of chlorophyll yielded increased levels of phytanic acid in ruminant products; however, definitive quantitative studies had not yet been conducted. Two in vitro batch culture experiments were conducted using rumen fluid collected from a fistulated non-lactating Holstein, fed 100% forage diet 10 days prior to sampling. Rumen fluid was strained through double layered cheesecloth, mixed with artificial saliva in a 2:1 ratio, rumen fluid to saliva, 30 mL was added directly to bottles containing fresh forage alfalfa or alfalfa plus three levels (1000, 2000, 3000 ppm) of phytol or chlorophyll. During the addition of the rumen inoculum the batch culture bottles were purged with CO2, tightly capped using rubber septums and crimp tops and placed in a 39° C water bath for specified time intervals. Upon the conclusion of the subsequent time periods, bottles were placed on ice to halt the fermentation process and placed in a refrigerator (4° C) until subsequent analysis.
    [Show full text]
  • Phytanic Acid, a Daily Consumed Chlorophyll-Yielded Phytol Bio-Metabolite: a Comprehensive Review
    Vol. 10(48), pp. 1025-1033, 29 December, 2016 DOI: 10.5897/AJPP2015.4312 Article Number: 2A23B6462203 African Journal of Pharmacy and ISSN 1996-0816 Copyright © 2016 Pharmacology Author(s) retain the copyright of this article http://www.academicjournals.org/AJPP Review Phytanic acid, a daily consumed chlorophyll-yielded phytol bio-metabolite: A comprehensive review Md. Torequl Islam1,2,3*, Eunus S. Ali4, Ricardo M. de Carvalho2, Márcia F. C. J. Paz2, Antonio L. Braga2, Rosália M. T. de Lima2, Leonardo da R. Sousa2, Marcus V. O. B. de Alencar1,2, Jose V. de Oliveira Santos2 and Ana A. de Carvalho Melo-Cavalcante1,2 1Northeast Biotechnology Network (RENORBIO), Postgraduate Program in Biotechnology, Federal University of Piauí, Teresina (Piaui)-64.049-550, Brazil. 2Laboratory of Toxicology and Genetics, Federal University of Piauí, Teresina (Piaui)-64.049-550, Brazil. 3Department of Pharmacy, Southern University Bangladesh, Mehedibag (Chittagong)-4000, Bangladesh. 4Gaco Pharmaceuticals and Research Laboratory, Dhaka-1000, Bangladesh. Received 6 March 2015; Accepted 9 September, 2016 An important chlorophyll-derived diterpenoid essential oil, phytol (PYT) bio-metabolite, phytanic acid (PA) has a number of pathophysiological contributions. The PA metabolism and its plasma levels associated phenomena are continuously being researched on. This study aims to look at the complete current scenario of PA. The findings suggest that PA has anti-diabetic, cytotoxic, anticancer and anti- teratogenic activities. Although PA-mediated Refsum’s Diseases (RD), Sjogren-Larsson Syndrome (SLS) and prostate cancer are still controversial; Zellweger's Disease Hyperpipecolic Academia (ZDHA), Rhizomelic Chondrodysplasia Punctata (RCDP), Leber Disease (LD) and oxidative stress leading to mitochondrial and cardiac complications are also evident.
    [Show full text]