bioRxiv preprint doi: https://doi.org/10.1101/342006; this version posted June 8, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 Uneven distribution of cobamide biosynthesis and dependence in bacteria predicted by 2 comparative genomics 3 4 Running title: Cobamide biosynthesis predictions 5 6 Amanda N. Shelton1, Erica C. Seth1, Kenny C. Mok1, Andrew W. Han2, Samantha N. Jackson3,4, 7 David R. Haft3, Michiko E. Taga1* 8 9 1 Department of Plant & Microbial Biology, University of California, Berkeley, Berkeley, CA, 10 USA 11 2 Second Genome, Inc., South San Francisco, CA, USA 12 3 J. Craig Venter Institute, Rockville, MD, USA 13 4 Current address: Department of Biological & Environmental Engineering, Cornell University, 14 Ithaca, NY, USA 15 16 * Address correspondence to Michiko E. Taga,
[email protected] 17 18 19 -- 20 Abstract 21 22 The vitamin B12 family of cofactors known as cobamides are essential for a variety of microbial 23 metabolisms. We used comparative genomics of 11,000 bacterial species to analyze the extent 1 bioRxiv preprint doi: https://doi.org/10.1101/342006; this version posted June 8, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 24 and distribution of cobamide production and use across bacteria.