DOCSLIB.ORG

Filmarray® Blood Culture Identification Panel Quick Guide

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Filmarray® Blood Culture Identification Panel Quick Guide ® FilmArray Blood Culture Identification Panel Quick Guide Testing on the FilmArray should be performed within 8 hours of the positive reading from a continuously monitoring blood culture instrument. To avoid contamination always wear gloves and work behind a protective shield. Step 1: Prepare Pouch Once sample is ready for testing, remove pouch from packaging. Insert pouch into Pouch Loading Station. Place Sample Buffer vial into red well. OR Place Hydration Solution vial into blue well. Step 2: Hydrate Pouch Draw 1 mL of Hydration Solution into Hydration Syringe. If bubbles are present in liquid, leave syringe tip in vial and gently tap side of syringe, releasing bubbles to float to surface. Insert Hydration Syringe tip into hydration port of pouch located OR directly below blue arrow. Holding the barrel of the Hydration Syringe, forcefully push down to puncture port seal. DO NOT PUSH THE SYRINGE PLUNGER. FilmArray Wait as Hydration Solution is drawn into pouch. ™ Panel Note: See FilmArray Operator's Manual Troubleshooting section if pouch fails to hydrate. OR Step 3: Prepare Sample Mix Use a 28 gauge needle to withdraw 0.1 mL of sample from blood culture bottle. Transfer sample to Sample Buffer vial. Gently mix up and down using Transfer Pipette. Note: For alternate workflow, see package insert. FilmArray ™ Panel Step 4: Load Sample Mix OR Draw 0.3 mL of Sample Mix using Sample Loading Syringe. Insert Sample Loading Syringe tip into sample port of pouch FilmArray located directly below red arrow. ™ Panel Holding the barrel of the syringe, forcefully push down to puncture port seal. DO NOT PUSH THE SYRINGE PLUNGER. Wait as Sample Mix is drawn into pouch. Step 5: Run Pouch Follow instructions on software screen. FilmArray The pouch will click into place when properly seated. ™ Panel Note: If the pouch does not insert easily, ensure that the lid is opened completely. ® BioFire Diagnostics, LLC | 390 Wakara Way, Salt Lake City, Utah 84108, USA | 1-801-736-6354 ® FilmArray Blood Culture Identification Panel Quick Guide TM BCID Panel The Run Summary Section displays information Run Summary about the sample and a summary of the control and Sample ID: 8017366354 Run Date: 19 Mar 2012 3:36 PM test results. 1. Organisms Staphylococcus 3. Controls: Passed Detected: Staphylococcus aureus 1. Organisms Detected: Applicable • Names of any detected organisms 2. • If "None", no organisms were detected Antimicrobial mecA - Detected Resistance Genes: • If " Invalid", RETEST SAMPLE 2. Applicable Antimicrobial Resistance Genes: Displays results for antimicrobial resistance gene(s) associated with detected organism(s). Results Summary - Interpretations 3. Controls: Antimicrobial Resistance Genes • If "Passed", results are valid √ Detected mecA (methicillin-resistance gene) • If " Failed" or " Invalid", RETEST SAMPLE 4. Ø N/A vanA/B (vancomycin-resistance genes) Ø N/A KPC (carbapenem-resistance gene) NOTE: Antimicrobial resistance can occur via multiple mechanisms. A Not Detected result for the FilmArray antimicrobial resistance gene assays does not indicate antimicrobial susceptibility. Subculturing is required for species identification and susceptibility testing of isolates. Gram Positive Bacteria The Results Summary-Interpretations Section Not Detected Enterococcus lists the test results for each organism or antimicrobial Not Detected Listeria monocytogenes resistance gene targeted by the Blood Culture 5. √ Detected Staphylococcus Identification Panel. √ Detected Staphylococcus aureus 4. If "N/A", antimicrobial resistance gene was not Not Detected Streptococcus 6. reported because an organism associated with Not Detected Streptococcus agalactiae (Group B) the gene was not detected Not Detected Streptococcus pneumoniae Not Detected Streptococcus pyogenes (Group A) 5. If "Detected", organism or antimicrobial Gram Negative Bacteria resistance gene was detected Not Detected Acinetobacter baumannii 6. If "Not Detected", organism or antimicrobial Not Detected Enterobacteriaceae resistance gene was not detected Not Detected Enterobacter cloacae complex Not Detected Escherichia coli If “ Invalid”, RETEST SAMPLE Not Detected Klebsiella oxytoca Not Detected Klebsiella pneumoniae Note: If repeated "Invalid" results are obtained, Not Detected Proteus contact BioFire Diagnostics, the local bioMérieux Not Detected Serratia marcescens Not Detected Haemophilus influenzae sales representative, or an authorized distributor. Not Detected Neisseria meningitidis Not Detected Pseudomonas aeruginosa The Run Details Section displays information about Yeast the pouch, instrument, run status, and operator. Not Detected Candida albicans Not Detected Candida glabrata 7. Run Status: If Completed, run is complete. Not Detected Candida krusei If "Incomplete", "Aborted", "Instrument Not Detected Candida parapsilosis Communication Error", "Instrument Error", or Not Detected Candida tropicalis "Software Error", RETEST SAMPLE : If repeated "Error" messages are obtained, Note Run Details contact BioFire Diagnostics, the local bioMérieux Pouch: BCID Panel v2.0 Protocol: BC v 2.1 sales representative, or an authorized distributor. 7. Run Status: Completed Operator: Operator Serial No.: 00169044 Instrument: ITI FA "FA1187" Lot No.: 120228B ® © Copyright 2015 BioFire Diagnostics, LLC. All rights reserved. RFIT-PRT-0056-04 BioFire Diagnostics, LLC | 390 Wakara Way, Salt Lake City, Utah 84108, USA | 1-801-736-6354 RFIT-ASY-0114 RFIT-ASY-0109 FilmArray® Blood Culture Identification (BCID) Panel Instruction Booklet For use with the syringe-based loading system Customer and Technical Support for U.S. Customers Reach Us on the Web Reach Us by Phone http://www.BioFireDX.com 1-800-735-6544 – Toll Free (801) 736-6354 – Utah Reach Us by E-mail [email protected] Reach Us by Fax Reach Us by Mail (801) 588-0507 390 Wakara Way Salt Lake City, UT 84108 USA Customer and Technical Support outside of the U.S. Contact your local sales representative or distributor for technical support BioFire Diagnostics, LLC 390 Wakara Way Qarad b.v.b.a Cipalstraat 3 Salt Lake City, UT 84108 B-2440 Geel, USA Belgium © Copyright 2007–2015, BioFire Diagnostics, LLC. All rights reserved. RFIT-PRT-0101-04 February 2015 The information contained in this document is subject to change without notice. No part of this document may be reproduced or transmitted in any form or by any means, electronic or mechanical, for any purpose, without the express written permission of BioFire Diagnostics, LLC. FilmArray Software, Detector, and Metacall software modules © 2002–2015 BioFire Diagnostics, LLC. BioFire Diagnostics, BioFire, the BioFire logo, FilmArray and LCGreen are trademarks of BioFire Diagnostics, LLC or BioFire Defense, LLC and are registered trademarks in the United States. All other names of products and brands appearing in this manual are trademarks or registered trademarks of their respective owners. The purchase of this product includes a limited, nontransferable license under specific claims of one or more U.S. patents as listed on BioFire Diagnostics’ Web site (http://www.biofiredx.com/LegalNotices/) and owned by BioFire and the University of Utah Research Foundation. BioFire Diagnostics, LLC FilmArray BCID Panel CE-IVD Instruction Booklet i TABLE OF SYMBOLS The following symbols can be found on FilmArray BCID Panel Kit components or throughout this Instruction Booklet. Use the definitions below as a guideline to interpreting the symbols. Table of Symbols Expiry Date Manufacturer Catalog Number YYYY-MM-DD Consult Storage Temperature Lot Number Instructions for Use Limitations European Union Contains Sufficient Serial Number Conformity n For <n> Tests In vitro Diagnostic Keep Away from Do Not Reuse Medical Device Sunlight Acute toxicity, cat. Serious eye Do Not Use if 4 & Skin irritation, damage, cat. 1 Package is Damaged cat. 2 BioFire Diagnostics, LLC FilmArray BCID Panel CE-IVD Instruction Booklet ii TABLE OF CONTENTS TABLE OF SYMBOLS ................................................................................................................................................... II TABLE OF CONTENTS ................................................................................................................................................ III NAME AND INTENDED USE .........................................................................................................................................5 FILMARRAY BLOOD CULTURE IDENTIFICATION (BCID) PANEL ......................................................................................................... 5 SUMMARY AND EXPLANATION OF THE TEST ...............................................................................................................5 BACKGROUND OF TARGETED ORGANISMS ................................................................................................................................... 6 PRINCIPLE OF THE PROCEDURE ................................................................................................................................................. 11 MATERIALS PROVIDED ............................................................................................................................................. 12 MATERIALS REQUIRED BUT NOT PROVIDED .............................................................................................................. 13 WARNINGS AND PRECAUTIONS ...............................................................................................................................
Load more

Recommended publications
  • Hickman Catheter-Related Bacteremia with Kluyvera
    Jpn. J. Infect. Dis., 61, 229-230, 2008 Short Communication Hickman Catheter-Related Bacteremia with Kluyvera cryocrescens: a Case Report Demet Toprak, Ahmet Soysal, Ozden Turel, Tuba Dal1, Özlem Özkan1, Guner Soyletir1 and Mustafa Bakir* Department of Pediatrics, Section of Pediatric Infectious Diseases and 1Department of Microbiology, Marmara University School of Medicine, Istanbul, Turkey (Received September 10, 2007. Accepted March 19, 2008) SUMMARY: This report describes a 2-year-old child with neuroectodermal tumor presenting with febrile neu- tropenia. Blood cultures drawn from the peripheral vein and Hickman catheter revealed Kluyvera cryocrescens growth. The Hickman catheter was removed and the patient was successfully treated with cefepime and amikacin. Isolation of Kluyvera spp. from clinical specimens is rare. This saprophyte microorganism may cause serious central venous catheter infections, especially in immunosuppressed patients. Clinicians should be aware of its virulence and resistance to many antibiotics. Central venous catheters (CVCs) are frequently used in confirmed by VITEK AMS (VITEK Systems, Hazelwood, Mo., patients with hematologic and oncologic disorders. Along with USA) and by API (Analytab Inc., Plainview, N.Y., USA). Anti- their increased use, short- and long-term complications of microbial susceptibility was assessed by the disc diffusion CVCs are more often being reported. The incidence of CVC method. K. cryocrescens was sensitive for cefotaxime, cefepime, infections correlates with duration of catheter usage, immuno- carbapenems, gentamycine, amikacin and ciprofloxacin. logic status of the patient, type of catheter utilized and mainte- Intravenous cefepime and amikacin were continued and the nance techniques employed. A definition of CVC infection CVC was removed. His echocardiogram was normal and has been difficult to establish because of problems differen- a repeat peripheral blood culture was sterile 48 h after the tiating contaminant from pathogen microorganisms.
    [Show full text]
  • Intramammary Infections with Coagulase-Negative Staphylococcus Species
    Printing of this thesis was financially supported by Printed by University Press, Zelzate ISBN number: 9789058642738 INTRAMAMMARY INFECTIONS WITH COAGULASE-NEGATIVE STAPHYLOCOCCUS SPECIES IN BOVINES - MOLECULAR DIAGNOSTICS AND EPIDEMIOLOGY - KARLIEN SUPRÉ 2011 PROMOTORS/PROMOTOREN Prof. dr. Sarne De Vliegher Faculteit Diergeneeskunde, UGent Prof. dr. Ruth N. Zadoks Royal (Dick) School of Veterinary Studies, University of Edinburgh; Moredun Research Institute, Penicuik, Schotland Prof. dr. Freddy Haesebrouck Faculteit Diergeneeskunde, UGent MEMBERS OF THE EXAMINATION COMMITTEE/LEDEN VAN DE EXAMENCOMMISSIE Prof. dr. dr. h. c. Aart de Kruif Voorzitter van de examencommissie Prof. dr. Mario Vaneechoutte Faculteit Geneeskunde en Gezondheidswetenschappen, UGent Dr. Margo Baele Directie Onderzoeksaangelegenheden, UGent Dr. Lic. Luc De Meulemeester MCC-Vlaanderen, Lier Prof. dr. Geert Opsomer Faculteit Diergeneeskunde, UGent Prof. dr. Marc Heyndrickx Instituut voor Landbouw en Visserijonderzoek (ILVO), Melle Dr. Suvi Taponen University of Helsinki, Finland Prof. dr. Ynte H. Schukken Cornell University, Ithaca, USA INTRAMAMMARY INFECTIONS WITH COAGULASE-NEGATIVE STAPHYLOCOCCUS SPECIES IN BOVINES - MOLECULAR DIAGNOSTICS AND EPIDEMIOLOGY - KARLIEN SUPRÉ Department of Reproduction, Obstetrics, and Herd Health Faculty of Veterinary Medicine, Ghent University Dissertation submitted in the fulfillment of the requirements for the degree of Doctor in Veterinary Sciences, Faculty of Veterinary Medicine, Ghent University INTRAMAMMAIRE INFECTIES MET COAGULASE-NEGATIEVE
    [Show full text]
  • Food Microbiology Changes in the Microbial Communities in Vacuum
    Food Microbiology 77 (2019) 26–37 Contents lists available at ScienceDirect Food Microbiology journal homepage: www.elsevier.com/locate/fm Changes in the microbial communities in vacuum-packaged smoked bacon during storage T ∗ Xinfu Lia,b,d, Cong Lia,b,d, Hua Yea,b, Zhouping Wanga,b, Xiang Wud, Yanqing Hand, Baocai Xub,c,d, a State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China b School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China c School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China d State Key Laboratory of Meat Processing and Quality Control, Yurun Group, Nanjing, 211806, China ARTICLE INFO ABSTRACT Keywords: This study aimed to gain deeper insights into the microbiota composition and population dynamics, monitor the Microbial communities dominant bacterial populations and identify the specific spoilage microorganisms (SSOs) of vacuum-packed Smoked bacon bacon during refrigerated storage using both culture-independent and dependent methods. High-throughout High-throughput sequencing (HTS) sequencing (HTS) showed that the microbial composition changed greatly with the prolongation of storage time. The diversity of microbiota was abundant at the initial stage then experienced a continuous decrease. Lactic acid bacteria (LAB) mainly Leuconostoc and Lactobacillus dominated the microbial population after seven days of storage. A total of 26 isolates were identified from different growth media using traditional cultivation isolation and identification method. Leuconostoc mesenteroides and Leuconostoc carnosum were the most prevalent species since day 15, while Lactobacillus sakei and Lactobacillus curvatus were only found on day 45, suggesting that they could be responsible for the spoilage of bacon.
    [Show full text]
  • Virulence Factors in Staphylococcus Associated with Small Ruminant Mastitis: Biofilm Production and Antimicrobial Resistance Genes
    antibiotics Article Virulence Factors in Staphylococcus Associated with Small Ruminant Mastitis: Biofilm Production and Antimicrobial Resistance Genes Nara Cavalcanti Andrade 1, Marta Laranjo 1 , Mateus Matiuzzi Costa 2 and Maria Cristina Queiroga 1,3,* 1 MED–Mediterranean Institute for Agriculture, Environment and Development, Instituto de Investigação e Formação Avançada, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, Portugal; [email protected] (N.C.A.); [email protected] (M.L.) 2 Federal University of the São Francisco Valley, BR 407 Highway, Nilo Coelho Irrigation Project, s/n C1, Petrolina 56300-000, PE, Brazil; [email protected] 3 Departamento de Medicina Veterinária, Escola de Ciências e Tecnologia, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, Portugal * Correspondence: [email protected]; Tel.: +351-266-740-800 Abstract: Small ruminant mastitis is a serious problem, mainly caused by Staphylococcus spp. Different virulence factors affect mastitis pathogenesis. The aim of this study was to investigate virulence factors genes for biofilm production and antimicrobial resistance to β-lactams and tetracyclines in 137 staphylococcal isolates from goats (86) and sheep (51). The presence of coa, nuc, bap, icaA, icaD, blaZ, mecA, mecC, tetK, and tetM genes was investigated. The nuc gene was detected in all S. aureus isolates and in some coagulase-negative staphylococci (CNS). None of the S. aureus isolates carried the bap gene, while 8 out of 18 CNS harbored this gene. The icaA gene was detected in Citation: Andrade, N.C.; Laranjo, M.; S. aureus and S. warneri, while icaD only in S. aureus. None of the isolates carrying the bap gene Costa, M.M.; Queiroga, M.C.
    [Show full text]
  • (51) International Patent Classification: C12R 1/44 (2006.01) A23L 5/41
    ( (51) International Patent Classification: Published: C12R 1/44 (2006.01) A23L 5/41 (2016.01) — with international search report (Art. 21(3)) A23L 29/00 (20 16.0 1) A23L 13/40 (20 16.01) — with (an) indication(s) in relation to deposited biological (21) International Application Number: material furnished under Rule 13bis separately from the PCT/EP20 19/06 1422 description (Rules 13bis.4(d)(i) and 48.2(a) (viii)) (22) International Filing Date: 03 May 2019 (03.05.2019) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 18170807.4 04 May 2018 (04.05.2018) EP 18184186.7 18 July 2018 (18.07.2018) EP (71) Applicant: CHR. HANSEN A/S [DK/DK]; Boege Alle 10-12, 2970 Hoersholm (DK). (72) Inventors: THORSEN, Tina Mailing; c/o Chr. Hansen A/S, Boege Alle 10-12, 2970 Hoersholm (DK). BAROI, George Nabin; c/o Chr. Hansen A/S, Boege Alle 10-12, 2970 Hoersholm (DK). TAPONEN, Robin; c/o Chr. Hansen A/S, Boege Alle 10-12, 2970 Hoersholm (DK). SOELTOFT-JENSEN, Jakob; c/o Chr. Hansen A/S, Boege Alle 10-12, 2970 Hoersholm (DK). YDE, Birgitte; c/o Chr. Hansen A/S, Boege Alle 10-12, 2970 Hoersholm (DK). (81) Designated States (unless otherwise indicated, for every kind of national protection available) : AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
    [Show full text]
  • Table of Contents
    An Investigation of Staphylococcus aureus and Related Species From Flood Affected and Other Environmental Sources A Thesis in Molecular Microbiology by Nadeesha Samanmalee Jayasundara BSc (Environmental Conservation & Management) School of Biomedical Science, Institute of Health & Biomedical Innovation Queensland University of Technology Brisbane, Australia Thesis submitted to Queensland University of Technology in fulfilment of the requirements for the degree of Masters of Applied Science (Research) May 2014 2 Abstract The genus Staphylococcus consists of 45 species and is widely distributed across environments such as skin and mucous membranes of humans and animals, as well as in soil, water and air. S. aureus and S. epidermidis are the most commonly associated species with human infections. Hence, most studies have focused on clinical and clinically sourced staphylococci. In addition, S. haemoliticus, S. intermidius, S. delphini, and S. saprophiticus are also considered potentially pathogenic members of the genus. Although staphylococci are distributed in various environments, there have been very few studies examining residential air as a reservoir of clinically significant pathogens, particularly Staphylococcus species. As a result, airborne transmission of staphylococci, and associated health risks, remains unclear. This study included not only residential air but also air samples from flood affected houses. Flood water can be considered as a potential carrier of pathogenic bacteria, because flood water can be affected by residential septic systems, municipal sanitary sewer systems, hospital waste, agricultural lands/operations and wastewater treatment plants. Even after the flood waters recede, microorganisms that are transported in water can remain in soil, in or on plant materials and on numerous other surfaces. Therefore, there is a great concern for use of previously flooded indoor and outdoor areas.
    [Show full text]
  • Current Challenges of Veterinary Microbiological
    POST. MIKROBIOL., CURRENT CHALLENGES OF VETERINARY MICROBIOLOGICAL 2018, 57, 3, 270–277 http://www.pm.microbiology.pl DIAGNOSTICS CONCERNING THE SUSCEPTIBILITY DOI: 10.21307/PM-2018.57.3.270 OF STAPHYLOCOCCI TO ANTIBIOTICS Magdalena Kizerwetter-Świda*, Dorota Chrobak-Chmiel, Magdalena Rzewuska Division of Microbiology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Poland Submitted in April, accepted in May 2018 Abstract: Staphylococci belong to bacteria often isolated from clinical material obtained from animals. Unlike in human medicine, in veterinary, different species of coagulase-positive staphylococci are isolated from clinical specimens, and exceptStaphylococcus aureus, Staphylococcus pseudintermedius, and other species are also often recognized. Recently, the taxonomy of staphylococci has been updated, therefore, now it is necessary to recognize the new species as well. Currently, coagulase-negative staphylococci are considered an important group of opportunistic pathogens. The accurate identification of species within the genus Staphylococcus is important because, according to the EUCAST and CLSI recommendations, the interpretation of the results of susceptibility testing for S. aureus and coagulase-negative staphylococci is different. Furthermore, the resistance to methicillin in S. aureus strains is detected using a cefoxitin disk, whereas in the case of S. pseudintermedius – using an oxacillin disk. An important problem for veterinary microbiological laboratories
    [Show full text]
  • Possible Detection of Pathogenic Bacterial Species Inhabiting Streams in Great Smoky Mountains National Park
    POSSIBLE DETECTION OF PATHOGENIC BACTERIAL SPECIES INHABITING STREAMS IN GREAT SMOKY MOUNTAINS NATIONAL PARK A thesis presented to the faculty of the Graduate School of Western Carolina University in partial fulfillment of the requirement for the degree of Master of Science in Biology. By Kwame Agyapong Brown Director: Dr. Sean O’Connell Associate Professor of Biology and Biology Department Head Committee members: Dr. Sabine Rundle and Dr. Timothy Driscoll November 2016 ACKNOWLEDGEMENTS I would like to express my deepest gratitude to my adviser Dr. Sean O’Connell for his insightful mentoring and thoughtful contribution throughout this research. I would also like to acknowledge my laboratory teammates: Lisa Dye, Rob McKinnon, Kacie Fraser, and Tori Carlson for their diverse contributions to this project. This project would not have been possible without the generous support of the Western Carolina University Biology Department and stockroom; so I would like to thank the entire WCU biology faculty and Wesley W. Bintz for supporting me throughout my Masters program. Finally, I would like to thank my thesis committee members and reader: Dr. Sabine Rundle, Dr. Timothy Driscoll and Dr. Anjana Sharma for their contributions to this project. ii TABLE OF CONTENTS List of Tables ................................................................................................................................. iv List of Figures ..................................................................................................................................v
    [Show full text]
  • Bacteria Richness and Antibiotic-Resistance in Bats from a Protected Area in the Atlantic Forest of Southeastern Brazil
    RESEARCH ARTICLE Bacteria richness and antibiotic-resistance in bats from a protected area in the Atlantic Forest of Southeastern Brazil VinõÂcius C. ClaÂudio1,2,3*, Irys Gonzalez2, Gedimar Barbosa1,2, Vlamir Rocha4, Ricardo Moratelli5, FabrõÂcio Rassy2 1 Centro de Ciências BioloÂgicas e da SauÂde, Universidade Federal de São Carlos, São Carlos, SP, Brazil, 2 FundacËão Parque ZooloÂgico de São Paulo, São Paulo, SP, Brazil, 3 Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil, 4 Centro de Ciências AgraÂrias, Universidade Federal de São Carlos, Araras, SP, Brazil, 5 Fiocruz Mata AtlaÃntica, FundacËão Oswaldo Cruz, Rio de Janeiro, RJ, a1111111111 Brazil a1111111111 [email protected] a1111111111 * a1111111111 a1111111111 Abstract Bats play key ecological roles, also hosting many zoonotic pathogens. Neotropical bat microbiota is still poorly known. We speculate that their dietary habits strongly influence OPEN ACCESS their microbiota richness and antibiotic-resistance patterns, which represent growing and Citation: ClaÂudio VC, Gonzalez I, Barbosa G, Rocha serious public health and environmental issue. Here we describe the aerobic microbiota V, Moratelli R, Rassy F (2018) Bacteria richness richness of bats from an Atlantic Forest remnant in Southeastern Brazil, and the antibiotic- and antibiotic-resistance in bats from a protected area in the Atlantic Forest of Southeastern Brazil. resistance patterns of bacteria of clinical importance. Oral and rectal cavities of 113 bats PLoS ONE 13(9): e0203411. https://doi.org/ from Carlos Botelho State Park were swabbed. Samples were plated on 5% sheep blood 10.1371/journal.pone.0203411 and MacConkey agar and identified by the MALDI-TOF technique.
    [Show full text]
  • Interplay of Virulence, Antibiotic Resistance and Epidemiology in Escherichia Coli Clinical Isolates
    Interplay of virulence, antibiotic resistance and epidemiology in Escherichia coli clinical isolates Elisabet Guiral Vilalta Aquesta tesi doctoral està subjecta a la llicència Reconeixement- NoComercial – SenseObraDerivada 4.0. Espanya de Creative Commons. Esta tesis doctoral está sujeta a la licencia Reconocimiento - NoComercial – SinObraDerivada 4.0. España de Creative Commons. This doctoral thesis is licensed under the Creative Commons Attribution-NonCommercial- NoDerivs 4.0. Spain License. Facultat de Medicina Departament de Fonaments Clínics Programa de Doctorat de Medicina i Recerca Translacional “Interplay of virulence, antibiotic resistance and epidemiology in Escherichia coli clinical isolates” Doctoranda: Elisabet Guiral Vilalta Departament de Fonaments Clínics Institut de Salut Global de Barcelona‐ Universitat de Barcelona‐ Hospital Clínic de Barcelona Directors de tesi: Dr. Jordi Vila Estapé i Dra. Sara M. Soto González Departament de Fonaments Clínics Institut de Salut Global de Barcelona‐ Universitat de Barcelona‐ Hospital Clínic de Barcelona Barcelona, Setembre 2018 El Dr. JORDI VILA ESTAPÉ, Catedràtic del Departament de Fonaments Clínics de la Facultat de Medicina de la Universitat de Barcelona, Cap del Servei de Microbiologia de l’Hospital Clínic de Barcelona i Research Professor i Director de la Iniciativa de Resistències Antimicrobianes de l’Institut de Salut Global de Barcelona (ISGlobal) i la Dra. SARA M. SOTO GONZÁLEZ, Professora Associada del Departament de Fonaments Clínics de la Universitat de Barcelona i Associate Research Professor d’ ISGlobal, CERTIFIQUEN: Que el treball de recerca titulat “Interplay of virulence, antibiotic resistance and epidemiology in Escherichia coli clinical isolates”, presentat per ELISABET GUIRAL VILALTA, ha estat realitzat al Laboratori de Microbiologia de l’ISGlobal, dins les dependències de l’Hospital Clínic de Barcelona, sota la seva direcció i compleix tots els requisits necessaris per la seva tramitació i posterior defensa davant del Tribunal corresponent.
    [Show full text]
  • The Genera Staphylococcus and Macrococcus
    Prokaryotes (2006) 4:5–75 DOI: 10.1007/0-387-30744-3_1 CHAPTER 1.2.1 ehT areneG succocolyhpatS dna succocorcMa The Genera Staphylococcus and Macrococcus FRIEDRICH GÖTZ, TAMMY BANNERMAN AND KARL-HEINZ SCHLEIFER Introduction zolidone (Baker, 1984). Comparative immu- nochemical studies of catalases (Schleifer, 1986), The name Staphylococcus (staphyle, bunch of DNA-DNA hybridization studies, DNA-rRNA grapes) was introduced by Ogston (1883) for the hybridization studies (Schleifer et al., 1979; Kilp- group micrococci causing inflammation and per et al., 1980), and comparative oligonucle- suppuration. He was the first to differentiate otide cataloguing of 16S rRNA (Ludwig et al., two kinds of pyogenic cocci: one arranged in 1981) clearly demonstrated the epigenetic and groups or masses was called “Staphylococcus” genetic difference of staphylococci and micro- and another arranged in chains was named cocci. Members of the genus Staphylococcus “Billroth’s Streptococcus.” A formal description form a coherent and well-defined group of of the genus Staphylococcus was provided by related species that is widely divergent from Rosenbach (1884). He divided the genus into the those of the genus Micrococcus. Until the early two species Staphylococcus aureus and S. albus. 1970s, the genus Staphylococcus consisted of Zopf (1885) placed the mass-forming staphylo- three species: the coagulase-positive species S. cocci and tetrad-forming micrococci in the genus aureus and the coagulase-negative species S. epi- Micrococcus. In 1886, the genus Staphylococcus dermidis and S. saprophyticus, but a deeper look was separated from Micrococcus by Flügge into the chemotaxonomic and genotypic proper- (1886). He differentiated the two genera mainly ties of staphylococci led to the description of on the basis of their action on gelatin and on many new staphylococcal species.
    [Show full text]
  • Molecular Characterization of Culturable Aerobic Bacteria in the Midgut of Field-Caught Culex Tritaeniorhynchus, Culex Gelidus, and Mansonia Annulifera Mosquitoes in the Gampaha
    Hindawi BioMed Research International Volume 2020, Article ID 8732473, 13 pages https://doi.org/10.1155/2020/8732473 Research Article Molecular Characterization of Culturable Aerobic Bacteria in the Midgut of Field-Caught Culex tritaeniorhynchus, Culex gelidus, and Mansonia annulifera Mosquitoes in the Gampaha District of Sri Lanka Nayana Gunathilaka ,1 Koshila Ranasinghe ,2 Deepika Amarasinghe ,2 Wasana Rodrigo,3 Harendra Mallawarachchi,4 and Nilmini Chandrasena1 1Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka 2Department of Zoology and Environmental Management, Faculty of Science, University of Kelaniya, Colombo, Sri Lanka 3Biotechnology Unit, Industrial Technology Institute, Colombo 07, Colombo, Sri Lanka 4Department of Parasitology and Medical Entomology, Medical Research Institute, Colombo 08, Colombo, Sri Lanka Correspondence should be addressed to Nayana Gunathilaka; [email protected] Received 5 June 2020; Revised 8 August 2020; Accepted 17 September 2020; Published 5 October 2020 Academic Editor: Wen Jun Li Copyright © 2020 Nayana Gunathilaka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. Larval and adult mosquito stages harbor different extracellular microbes exhibiting various functions in their digestive tract including host-parasite interactions. Midgut symbiotic bacteria can be genetically exploited to express molecules within the vectors, altering vector competency and potential for disease transmission. Therefore, identification of mosquito gut inhabiting microbiota is of ample importance before developing novel vector control strategies that involve modification of vectors. Method. Adult mosquitoes of Culex tritaeniorhynchus, Culex gelidus, and Mansonia annulifera were collected from selected Medical Officer of Health (MOH) areas in the Gampaha district of Sri Lanka.
    [Show full text]