SANTA CRUZ BIOTECHNOLOGY, INC. Oma1 (H-11): sc-515788

BACKGROUND STORAGE Oma1 (Oma1 homolog, zinc metallopeptidase (S. cerevisiae)), also known Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of as MPRP-1 or ZMPOMA1, is a 521 amino acid mitochondrial protease that shipment. Non-hazardous. No MSDS required. belongs to the peptidase M48 family. Oma1 is an integral part of the inner membrane, and it functions to mediate the proteolytic breakdown of a mis- DATA folded derivative of the polytopic inner membrane protein OXA1. Oma1 is A B a novel component of the quality control system in the inner membrane of ABCDE F mitochondria. The encoding Oma1 maps to human 1p32.2, 90 K – which spans 260 million base pairs, contains over 3,000 and com- 55 K – prises nearly 8% of the . houses a large num- 43 K – < Oma1 ber of disease-associated genes, including those that are involved in familial 34K– adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher 23 K – disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of cancers, including head and neck cancer, malignant melanoma and multiple myeloma. Oma1 (H-11): sc-515788. Western blot analysisof Oma1 (H-11): sc-515788. Immunoperoxidase staining Oma1 expressioninDaudi (A), HL-60 (B), K-562 (C), of formalin fixed, paraffin-embedded human kidney MEG-01(D), U-937(E) and U-698-M (F)wholecell tissue showing cytoplasmic staining of cells in CHROMOSOMAL LOCATION lysates. glomeruli and cells in tubules (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human Genetic locus: OMA1 (human) mapping to 1p32.2; Oma1 (mouse) mapping fallopian tube tissue showing cytoplasmic staining of glandular cells (B). to 4 C6. SELECT PRODUCT CITATIONS SOURCE 1. Ausman, J., et al. 2018. Ceramide-induced BOK promotes mitochondrial Oma1 (H-11) is a mouse monoclonal antibody raised against amino acids fission in preeclampsia. Cell Death Dis. 9: 298. 230-399 mapping within an internal region of Oma1 of human origin. 2. Lee, Y.J., et al. 2019. Down-regulation of the mitochondrial i-AAA protease PRODUCT Yme1L induces muscle atrophy via FoxO3a and myostatin activation. J. Cell. Mol. Med. 24: 899-909. Each vial contains 200 µg IgG2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. 3. Fung, T.S., et al. 2019. Two distinct actin filament populations have effects on mitochondria, with differences in stimuli and assembly factors. J. Cell Oma1 (H-11) is available conjugated to agarose (sc-515788 AC), 500 µg/ Sci. 132: jcs234435. 0.25 ml agarose in 1 ml, for IP; to HRP (sc-515788 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; to either phycoerythrin (sc-515788 PE), fluorescein 4. Tobacyk, J., et al. 2019. The first direct activity assay for the mitochondrial (sc-515788 FITC), Alexa Fluor® 488 (sc-515788 AF488), Alexa Fluor® 546 protease OMA1. 46: 1-5. (sc-515788 AF546), Alexa Fluor® 594 (sc-515788 AF594) or Alexa Fluor® 647 5. Sugawara, S., et al. 2020. The mitochondrial protein PGAM5 suppresses (sc-515788 AF647), 200 µg/ml, for WB (RGB), IF, IHC(P) and FCM; and to either energy consumption in brown adipocytes by repressing expression of Alexa Fluor® 680 (sc-515788 AF680) or Alexa Fluor® 790 (sc-515788 AF790), uncoupling protein 1. J. Biol. Chem. 295: 5588-5601. 200 µg/ml, for Near-Infrared (NIR) WB, IF and FCM. 6. Noh, S., et al. 2020. p32/C1QBP regulates OMA1-dependent proteolytic APPLICATIONS processing of OPA1 to maintain mitochondrial connectivity related to mito- chondrial dysfunction and apoptosis. Sci. Rep. 10: 10618. Oma1 (H-11) is recommended for detection of Oma1 of mouse, rat and human origin by Western Blotting (starting dilution 1:100, dilution range 7. Lee, H., et al. 2020. The short variant of optic atrophy 1 (OPA1) improves 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein cell survival under oxidative stress. J. Biol. Chem. 295: 6543-6560. (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution 8. Aishwarya, R., et al. 2020. Pleiotropic effects of mdivi-1 in altering mito- range 1:50-1:500), immunohistochemistry (including paraffin-embedded chondrial dynamics, respiration, and autophagy in cardiomyocytes. Redox sections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phase Biol. 36: 101660. ELISA (starting dilution 1:30, dilution range 1:30-1:3000). 9. Douida, A., et al. 2021. Cells lacking PA200 adapt to mitochondrial dysfunc- Suitable for use as control antibody for Oma1 siRNA (h): sc-78998, Oma1 tion by enhancing glycolysis via distinct Opa1 processing. Int. J. Mol. Sci. siRNA (m): sc-151297, Oma1 shRNA Plasmid (h): sc-78998-SH, Oma1 shRNA 22: 1629. Plasmid (m): sc-151297-SH, Oma1 shRNA (h) Lentiviral Particles: sc-78998-V and Oma1 shRNA (m) Lentiviral Particles: sc-151297-V. RESEARCH USE Molecular Weight of Oma1 precursor: 60 kDa. For research use only, not for use in diagnostic procedures.

Molecular Weight of cleaved Oma1: 40 kDa. Alexa Fluor® is a trademark of Molecular Probes, Inc., Oregon, USA

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