DOCSLIB.ORG

Molecular Typing of Eimeria Ahsata and E. Crandallis Isolated from Slaughterhouse Wastewater

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

Jundishapur J Microbiol. 2016 April; 9(4):e34140. doi: 10.5812/jjm.34140. Published online 2016 April 23. Letter Molecular Typing of Eimeria ahsata and E. crandallis Isolated From Slaughterhouse Wastewater Kareem Hatam Nahavandi,1 Amir Hossein Mahvi,2 Mehdi Mohebali,1,3 Hossein Keshavarz,1 Sasan Rezaei,1 Hamed Mirjalali,4,5 Samira Elikaei,1 and Mostafa Rezaeian1,* 1Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran 2Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran 3Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, IR Iran 4Gastroenterology and Liver Disease Research Center, Research institute for Gastroentrology and Liver Diseases, Shahid Beheshti University of Medical Sciences (SBUMS), Tehran, IR Iran 5Foodborne and Waterborne Diseases Research Center, Research institute for Gastroentrology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran *Corresponding author: Mostafa Rezaeian, Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran. Tel: +98-2188973901, E-mail: [email protected] Received 2015 October 28; Revised 2016 February 02; Accepted 2016 February 07. Keywords: 18S rRNA Gene, Iran, Wastewater, Eimeria ahsata, Eimeria crandallis Dear Editor, and DNA sequence variations of E. crandallis and E. ahsata compared with other Eimeria that exist in the GenBank The Eimeria species are host-specific protozoan para- database. Therefore, the present study was undertaken to sites that cause the disease known as coccidiosis in a va- identify genetic characteristics of E. ahsata and E. crandallis riety of animals including cattle, sheep, goats, pigs and in slaughterhouse wastewater samples. poultry throughout the world (1). These parasites invade epithelial tissues of the intestine, causing severe damage Twenty-four grab samples of untreated wastewater in the host and result in significant economic losses (2). (5 L each) were collected from the two slaughterhouse At least twelve species of bovine Eimeria, ten species of treatment plants located in the southwest suburban area ovine Eimeria and nine species of caprine Eimeria occur of Tehran, Iran. The animals slaughtered in these two in Iran (3-5). Eimeria ahsata and E. crandallis are two of a slaughterhouses included cattle, sheep and goats. Wa- group of ovine coccidia of what might be called the ar- ter samples were concentrated by the centrifugal (water- loingi type (6). Conventionally, microscopic examination ether) concentration procedure, as previously described of Eimerian oocytes is the only practical method to dis- (12). Oocytes were sporulated in a 2% (w/v) potassium criminate species of Eimeria (2,7,8). However, the partic- dichromate (K2Cr2O7) solution at room temperature (25°C) ular morphology of a given species may vary considerably. for three weeks. The sporulated oocytes were partially Accordingly, microscopic differentiation is not reliable be- identified as E. ahsata and E. crandallis based on the mor- cause several species have confusing features, along with phology of oocytes at × 40 and × 1,000 magnifications the presence of intraspecies variation (7). Therefore, mor- (13). DNA was extracted using an AccuPrep® stool DNA phological observations should not be used as isolated cri- extraction kit (Bioneer Corporation, Daejeon, South Ko- terion for differentiation of species (9). rea) according to the manufacturer’s protocol. An approx- imately 636-bp fragment of DNA extract was PCR amplified Thus, molecular assays have proven useful for the iden- on the basis of the 18S rRNA gene, as previously described tification of Eimeria spp. to overcome the limitations (14). All PCR amplicons were sequenced in both directions of these traditional procedures (7, 10). However, hema- on an automated DNA analyzer (ABI 3730 XL, Bioneer, South tological and biochemical changes in blood serum, and Korea). histopathological lesions of ovine coccidiosis in sheep and lambs naturally infected by E. crandallis and E. ahsata were Unique nucleotide sequences described in this work mentioned in previous studies (11), but no information were deposited in GenBank and accession numbers is available about the genetic characterization and phylo- KP893746, KP893747, KP893748 and KP893749 were pro- genic positions of E. crandallis and E. ahsata. In the present vided for Iranian E. ahsata and E. crandallis sequences of study, the 18S rRNA gene was employed as a molecular 18S rRNA. genetic approach to investigate the phylogenetic analysis Sporulated oocytes of E. ahsata and E. crandallis were Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. Hatam Nahavandi K et al. identified in all wastewater samples three weeks after their study, the microscopic examination resulted in positive re- storage at 25°C. All samples that were found to be positive sults for all water samples from both slaughterhouse treat- by microscopic evaluation were confirmed to be Eimeria ment plants. The presence of positive samples for E. ahsata by PCR. BLAST search of our 18S rRNA sequences against and E. crandallis was confirmed by PCR assay. those previously published for other Eimeria spp. revealed In the present study, 18S rRNA sequences of E. crandallis the highest similarity (99% - 100% homology) with E. ah- and E. ahsata had considerable homology with E. arloingi, sata and E. crandallis, with differences observed at 2 - 4 nu- E. bovis and E. zuernii (98% - 99%). Similarly, Khodakaram- cleotides. Genetic distances among Eimeria species were Tafti et al. (4) reported a close relationship between E. ar- in the range of 0.002 to 1.264 nucleotide substitutions per loingi and bovine (E. bovis and E. zuernii) and ovine (E. ah- 100 bp. There was a minor difference in the sequence of sata and E. crandallis) coccidia, based on the 18S sequence 18S rRNA gene in E. ahsata and E. crandallis with E. arloingi analyses. It has also been shown that there is a similar- available in GenBank. Phylogenetic analysis showed that ity between these two species (E. crandallis and E. ahsata) one clade contained E. ahsata, E. crandallis and E. arloingi and E. arloingi, based on microscopic evaluation of their (the most pathogenic Eimeria in goats), and E. ahsata and sporulated oocytes. Identically, E. ahsata and E. crandallis in E. crandallis grouped together in one clade (Figure 1). sheep, E. arloingi in goats and E. bovis and E. zuernii in cattle are highly pathogenic and formed a monophyletic group Figure 1. Phylogenetic Tree Based on 18S rRNA Sequences, Constructed According in the position away from other members in spite of many to the NJ Method, Showing the Position of E. ahsata, E. crandallis and Other Eimeria different biological characteristics and the histopatholog- Species Isosporasuis is Used as an Outgroup ical lesions. The phylogram based on the 18S rRNA se- quences showed that one clade contained E. ahsata, E. cran- 64 Eimeria Brunetti |U67116| 68 Eimeria Maxima |U67117| dallis and E. arloingi, and E. ahsata and E. crandallis grouped 44 Eimeria Acervulina |U67115| together in one clade. Similarly, Bush (16) reported a ten- 53 Eimeria Mitis |U67118| Eimeria Tenella |U67121| dency provided by the phylogenetic analysis of avian Eime- 37 99 Eimeria Necatrix |U67119| ria for E. necatrix and E. tenella, the most pathogenic Eimeria Eimeria Meleagrimitis |AF041437| Cyclospora Cayetanensis |AF111183| 15 58 in chicken. 93 Cyclospora Cercopitheci |AF111184| While the 18S rRNA sequence may not eventually prove 69 Cyclospora Papionis |AF111187| 6 Eimeria Pilarensis |AF324215| to be useful to examine in greater detail the magnitude of 71 Eimeria Separata |AF311643| population variation of a single Eimeria species, it can be Eimeria Langebarteli |AF311640| 2 86 Eimeria Nieschulzi |U40263| used to identify Eimerian oocytes in environmental sam- 85 Eimeria Falciformis |AF080614| 15 ples. 61 Eimeria Sevilletensis |AF311644| Eimeria Catronensis |AF324213| 35 Eimeria Alabamensis |AF291427| Eimeria Hessei |KC333453| Acknowledgments 58 Eimeria Dipodomysis |AF339490| 65 Eimeria Zuernii |AB769665| 86 Eimeria Ellipsoidalis |AB769634| The authors thank Fatemeh Tarighi and Tahereh Reza- Eimeria Bovis |U77084| eian for technical assistance. 98 Eimeria Arloingi |KC507792| Eimeria Ahsata |KP893747| 76 Eimeria Crandallis |KP893746| 50 Eimeria Crandallis |KP893748| Footnotes Eimeria Crandallis |KP893749| Eimeria Gruis |AB194382| Authors’ Contribution: The overall implementation of 99 Eimeria Reichenowi |AB194379| Isospora Suis |U97523| this study was the results of efforts of all authors. All au- 0.02 thors read and approved the final manuscript. Funding/Support: This work was supported by the insti- The percentage of replicate tree in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to the branches. tute for environmental research (IER) and center for re- search of endemic parasites of Iran (CREPI), Tehran Univer- sity of Medical Sciences (TUMS), Iran (grant no. 92-02-160- Prior to this
Recommended publications
  • Coccidiosis in Large and Small Ruminants

    Coccidiosis in Large and Small Ruminants

    Coccidiosis in Large and Small Ruminants a, b Sarah Tammy Nicole Keeton, PhD, MS *, Christine B. Navarre, DVM, MS KEYWORDS Coccidia Coccidiosis Diarrhea Ruminants Cattle Sheep Goats Ionophores KEY POINTS Coccidiosis is an important parasitic disease of ruminant livestock caused by the proto- zoan parasite of the genus Eimeria. Calves between 6 and 12 months of age and lambs and kids between 1 and 6 months of age are most susceptible. Subclinical disease is characterized by poor growth. Clinical disease is most commonly characterized by diarrhea. Control of coccidiosis is based on sound management, the use of preventive medications, and treatment of clinical cases as necessary. INTRODUCTION: NATURE OF THE PROBLEM Coccidiosis is a parasitic disease of vertebrate animals, including domestic ruminants.1 It is economically significant, with losses from both clinical and subclinical disease. Coccidiosis is caused by the protozoan parasite of the genus Eimeria. Eimeria are host specific, meaning that an Eimeria species that infect goats does not infect sheep or cattle and vice versa. Certain species of Eimeria are nonpathogenic and do not cause disease. The pathogenic species and sites of infection are listed in Table 1. Mixed infections with multiple pathogenic and nonpathogenic species is common. LIFE CYCLE Proper treatment and control of coccidiosis requires an understanding of the complex life cycle and transmission of Eimeria spp (Fig. 1). The life cycle can be divided into Disclosure: The authors have nothing to disclose. a Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman Drive, Baton Rouge, LA 70803, USA; b LSU AgCenter, School of Animal Sciences, Louisiana State University, 111 Dalrymple Bldg, 110 LSU Union Square, Baton Rouge, LA 70803-0106, USA * Corresponding author.
  • Coccidia (Protozoa: Apicomplexa) of the Domesticated

    Coccidia (Protozoa: Apicomplexa) of the Domesticated

    Copyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and private study only. The thesis may not be reproduced elsewhere without the permission of the Author. COCCIDIA (PROTOZOA: APICOMPLEXA) OF THE DOMESTICATED GOAT CAPRA HIRCUS IN NEW ZEALAND A THESIS PRESENTED IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF PHILOSOPHY IN VETERINARY SCIENCE AT MASSEY UNIVERSITY AYE KYAWT SOE SEPTEMBER, 1989 ~sey University Library Thesis Copyright Form Title of thesis: Cceu '/;)1/t ( PB97\2-?-o4 : A P1 CbM rl£x.A) 0 P ·n+t DOI\H~"t'"'TiCA--TE:D 6Dt-T C4Pr:ut t+1.e..c.u.s ft\J NN iEALA-NJ) (1) (a) I give permission for my thesis to be made available to readers in the Massey University Library under conditions determined by the Librarian. ")) I do not wish my thesis to be nade available to readers without my written consent for _______ rmnths. ( 2) (a) I agree that my thesis, or a copy, may be sent to another institution under conditions determined by the Librarian. (b) I do not wish my thesis, or a copy, to be sent to another institution without my written consent for· ------- roonths. (3) (a) I agree that my thesis nay be copied for Library use. (b) I do not wish my thesis to be copied for Library use for months. Signed Date The copyright of this thesis belongs to the author. Readers must sign their narre in the spaoe below to show that they recognise this • They are asked to add their pe:r:nanent address.
  • Observations on Sporulation of Eimeria Bovis (Apicomplexa: Eimeriidae) from the European Bison Bison Bonasus: Effect of Temperature and Potassium Dichromate Solution

    Observations on Sporulation of Eimeria Bovis (Apicomplexa: Eimeriidae) from the European Bison Bison Bonasus: Effect of Temperature and Potassium Dichromate Solution

    '>?? 82'%$32'2' doi: %'%11%%&2'%$'2' http://folia.paru.cas.cz Research note Observations on sporulation of Eimeria bovis (Apicomplexa: Eimeriidae) from the European bison Bison bonasus: effect of temperature and potassium dichromate solution Anna M. Pyziel and Aleksander W. Demiaszkiewicz L!# Abstract: "#!\!!! Eimeria bovis (K2Cr2O6B[ EFG%5'+B!!!; bison Bison bonasusEHBIE2$J!2M?B#! GEB#I!#!!%+?# 2+?9!E. bovis!2M?I!#E# IB5!!8I!%3### EMM$?B#IQIR2Cr2O6, besides the temperature, plays a crucial role in the process of sporulation of oocysts under laboratory conditions, as the longest delay in sporogony was observed when the faeces were stored without any other additives in the temperature of the refrigerator. Keywords:9!I!#>#!I8 Coccidia from the genus Eimeria %+6$ %5562''5BI E!9 ;!B # E. bovis was the most prevalent species of eimerians (prev* parasites of economic importance of livestock production, M'J7$'M3%$'!YQVZB due to increasing mortality rates of calves, reduction in ; G G !! food intake and decrease of body mass as well as body #GIE2'%1B !E8%5+'BH "GWI#! species of Eimeria are simple and begin when infective sporulation time of E. bovis, the most prevalent species of (sporulated) oocysts are ingested by a susceptible host. Eimeria, ;GEBison bonasus Linnaeus), 99#* I9! thelial cells of a host, but the symptoms of coccidiosis and "#G#2''6 #9 2'%% ! # ! I!ES!!%513B" !!;G of eimerian development, sporogony, takes place outside 8!#!G the host. of oocysts of E. bovis!EM%$'M3'''QVB# Time required for sporulation is one of diagnostic cri* placed in Petri dishes, which were being closed afterwards [GI* (to prevent dehydration of faecal material) and prepared G 9! ! for sporulation in different conditions.
  • Impact of Gastrointestinal Protozoan Infections on the Acute Phase Response in Neonatal Ruminants Seedetrakti Algloomnakkuste M

    Impact of Gastrointestinal Protozoan Infections on the Acute Phase Response in Neonatal Ruminants Seedetrakti Algloomnakkuste M

    TARMO NIINE TARMO IMPACT OF GASTROINTESTINAL PROTOZOAN INFECTIONS ON THE ACUTE PHASE RESPONSE IN NEONATAL RUMINANTS RESPONSE IN NEONATAL PHASE THE ACUTE INFECTIONS ON PROTOZOAN OF GASTROINTESTINAL IMPACT Professor IMPACT OF GASTROINTESTINAL PROTOZOAN THEINFECTIONS INFLUENCE ON THE OF ACUTEGROWTH PHASE CONDITIONS RESPONSE ONIN PHYSICO-MECHANICALNEONATAL RUMINANTS PROPERTIES OF SCOTS PINE (Pinus sylvestris L.) WOOD IN ESTONIA SEEDETRAKTI ALGLOOMNAKKUSTE MÕJU MÄLETSEJALISTE ÄGEDA JÄRGU VASTUSELE KASVUTINGIMUSTENEONATAALPERIOODIL MÕJU HARILIKU MÄNNI (Pinus sylvestris L.) PUIDU FÜÜSIKALIS- MEHAANILISTELE OMADUSTELE EESTIS TARMO NIINE REGINO KASK A thesis Professor Endla Reintam for applying for the degree of Doctor of Philosophy in VeterinaryA Thesis Sciences for applying for the degree of Doctor of Philosophy in Forestry Väitekiri filosoofiadoktori kraadi taotlemiseks loomaarstiteaduse erialal Väitekiri Dr. filosoofiadoktori kraadi taotlemiseks metsanduse erialal Tartu 20192015 Eesti Maaülikooli doktoritööd Doctoral Theses of the Estonian University of Life Sciences IMPACT OF GASTROINTESTINAL PROTOZOAN INFECTIONS ON THE ACUTE PHASE RESPONSE IN NEONATAL RUMINANTS SEEDETRAKTI ALGLOOMNAKKUSTE MÕJU MÄLETSEJALISTE ÄGEDA JÄRGU VASTUSELE NEONATAALPERIOODIL TARMO NIINE A thesis for applying for the degree of Doctor of Philosophy in Veterinary Sciences Väitekiri filosoofiadoktori kraadi taotlemiseks loomaarstiteaduse erialal Tartu 2019 Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences According to verdict No 3 of September 17, 2019, the Doctoral Committee of Veterinary and Food Science of Estonian University of Life Sciences has accepted the thesis for the defence of the degree of Doctor Philosophy in Veterinary Medicine and Food Science. Opponent: Prof. Bernd Lepenies Research Centre for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Germany. Supervisors: Prof. Toomas Orro Chair of Clinical Veterinary Medicine, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences.
  • The in Vitro and in Vivo Anti-Virulent Effect of Organic Acid Mixtures

    The in Vitro and in Vivo Anti-Virulent Effect of Organic Acid Mixtures

    www.nature.com/scientificreports OPEN The in vitro and in vivo anti‑virulent efect of organic acid mixtures against Eimeria tenella and Eimeria bovis Igori Balta1,2,3, Adela Marcu3, Mark Linton1, Carmel Kelly1, Lavinia Stef3,8*, Ioan Pet3,8*, Patrick Ward4, Gratiela Gradisteanu Pircalabioru5, Carmen Chifriuc5, Ozan Gundogdu6, Todd Callaway7,8* & Nicolae Corcionivoschi1,2,3,8* Eimeria tenella and Eimeria bovis are complex parasites responsible for the condition of coccidiosis, that invade the animal gastrointestinal intestinal mucosa causing severe diarrhoea, loss of appetite or abortions, with devastating impacts on the farming industry. The negative impacts of these parasitic infections are enhanced by their role in promoting the colonisation of the gut by common foodborne pathogens. The aim of this study was to test the anti‑Eimeria efcacy of maltodextrin, sodium chloride, citric acid, sodium citrate, silica, malic acid, citrus extract, and olive extract individually, in vitro and in combination, in vivo. Firstly, in vitro infection models demonstrated that antimicrobials reduced (p < 0.05), both singly and in combination (AG), the ability of E. tenella and E. bovis to infect MDBK and CLEC‑213 epithelial cells, and the virulence reduction was similar to that of the anti‑ coccidial drug Robenidine. Secondly, using an in vivo broiler infection model, we demonstrated that AG reduced (p = 0.001) E. tenella levels in the caeca and excreted faeces, reduced infammatory oxidative stress, improved the immune response through reduced ROS, increased Mn‑SOD and SCFA levels. Levels of IgA and IgM were signifcantly increased in caecal tissues of broilers that received 0.5% AG and were associated with improved (p < 0.0001) tissue lesion scores.
  • Prevalence of Eimeria Spp. in Calves from Dairy Farms in Northern Paraná State, Brazil Prevalência De Eimeria Spp

    Prevalence of Eimeria Spp. in Calves from Dairy Farms in Northern Paraná State, Brazil Prevalência De Eimeria Spp

    Short Communication ISSN 1984-2961 (Electronic) www.cbpv.org.br/rbpv Braz. J. Vet. Parasitol., Jaboticabal, v. 27, n. 1, p. 118-122, jan.-mar. 2018 Doi: http://dx.doi.org/10.1590/S1984-29612017072 Prevalence of Eimeria spp. in calves from dairy farms in northern Paraná state, Brazil Prevalência de Eimeria spp. em bezerros de propriedades leiteiras do norte do estado do Paraná, Brasil Sérgio Tosi Cardim1; Mércia Seixas1; Victor Bittencourt Dutra Tabacow1; Alessandra Taroda1; Priscilla Gomes Carneiro1; Thais Agostinho Martins1; Luiz Daniel de Barros1; Ana Flávia Minutti1; Andreas Lazaros Chryssafidis1; Odilon Vidotto1; João Luis Garcia1* 1 Laboratório de Protozoologia, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina – UEL, Londrina, PR, Brasil Received September 14, 2017 Accepted November 21, 2017 Abstract Bovine coccidiosis is a disease of major importance in cattle herds across the world. The disorder mainly affects young calves, and E. bovis and E. zuernii are considered the most pathogenic species of the genus, however, E. alabamensis have been described in grazing calves. In this study, the prevalence of Eimeria spp. was evaluated in calves on dairy farms in the northern region of the state of Paraná, Brazil. Four hundred calves on 44 dairy farms were tested for the presence of coccidian oocysts. The positives were re-examined and the oocysts were morphometrically analyzed for species identification. All the farms were contaminated and 205 animals (51.25%) presented Eimeria spp. oocysts. Among these, 146 animals (71.22%) were co-infected by two or more species of coccidia. Ten species of Eimeria were identified: E. bovis (in 30.25% of the positive samples), E.
  • EIMERIA SPECIES INFECTION in SURTI GOAT KIDS Priti D

    EIMERIA SPECIES INFECTION in SURTI GOAT KIDS Priti D

    International Journal of Science, Environment ISSN 2278-3687 (O) and Technology, Vol. 6, No 2, 2017, 1421 – 1425 2277-663X (P) EIMERIA SPECIES INFECTION IN SURTI GOAT KIDS Priti D. Vihol1*, J.M. Patel1, J.H. Patel2, J.K. Raval3, Y.R. Patel1, K.D. Thakor1 and P.P. Panchal1 1Department of Veterinary Pathology, 2Department of Veterinary Pharmacology & Toxicology, College of Veterinary Sciences & Animal Husbandry, Navsari Agricultural University, Eru cross road, Navsari-396 450, Gujarat, India. 3Department of TVCC, College of Veterinary Sciences & Animal Husbandry, Navsari Agricultural University, Eru cross road, Navsari-396 450, Gujarat, India E mail: [email protected] (*Corresponding Author) Abstract: Caprine coccidiosis, protozoan disease caused by Eimeria species, is major parasitic diseases characterized by contagious enteritis. Severity of Eimeria spp. infection is more in young animals. Two goat kids aged below one year died with history of mucoid to haemorrhagic yellow watery diarrhea, anorexia, emaciation, poor growth and death. At necropsy, gross lesions in intestines revealed diffuse small whitish non-pedunculated nodules of 5mm to 8mm diameter size in mucosa with focal petechial hemorrhages. Microscopic examination of intestinal content revealed numerous Eimeria spp. sporulated and unsporulated oocysts. In histopathological examination of intestines, enteritis was observed with denuded glandular epithelial cells, focal hyperplasia of intestinal villi and goblet cells, mild to moderate infiltration of inflammatory cells and haemorrhage. Various developmental stages of Eimeria spp. were also discernible in intestinal epithelial cells. Hence, caprine coccidiosis was diagnosed as the cause of death in kids. Keywords: Coccidiosis, Kids, Surti Goat. Introduction Coccidiosis, one of the economically important parasitic diseases of goats with worldwide distribution (Chartier and Paraud, 2012.) is caused by the apicomplexan protozoan parasite of genus Eimeria (Kimbita et al.
  • Nuclear, Plastid and Mitochondrial Genes for Dna Identification, Barcoding and Phylogenetics of Apicomplexan Parasites

    Nuclear, Plastid and Mitochondrial Genes for Dna Identification, Barcoding and Phylogenetics of Apicomplexan Parasites

    NUCLEAR, PLASTID AND MITOCHONDRIAL GENES FOR DNA IDENTIFICATION, BARCODING AND PHYLOGENETICS OF APICOMPLEXAN PARASITES A Thesis Presented to The Faculty of Graduate Studies of The University of Guelph by JOSEPH DAIRO OGEDENGBE In partial fulfillment of requirements for the degree of Doctor of Philosophy May, 2011 ©J. D. Ogedengbe,2011 Library and Archives Bibliotheque et 1*1 Canada Archives Canada Published Heritage Direction du Branch Patrimoine de I'edition 395 Wellington Street 395, rue Wellington Ottawa ON K1A 0N4 OttawaONK1A0N4 Canada Canada Your file Votre reference ISBN: 978-0-494-82844-1 Our file Notre reference ISBN: 978-0-494-82844-1 NOTICE: AVIS: The author has granted a non­ L'auteur a accorde une licence non exclusive exclusive license allowing Library and permettant a la Bibliotheque et Archives Archives Canada to reproduce, Canada de reproduire, publier, archiver, publish, archive, preserve, conserve, sauvegarder, conserver, transmettre au public communicate to the public by par telecommunication ou par Plnternet, preter, telecommunication or on the Internet, distribuer et vendre des theses partout dans le loan, distribute and sell theses monde, a des fins commerciales ou autres, sur worldwide, for commercial or non­ support microforme, papier, electronique et/ou commercial purposes, in microform, autres formats. paper, electronic and/or any other formats. The author retains copyright L'auteur conserve la propriete du droit d'auteur ownership and moral rights in this et des droits moraux qui protege cette these. Ni thesis. Neither the thesis nor la these ni des extraits substantiels de celle-ci substantial extracts from it may be ne doivent etre imprimes ou autrement printed or otherwise reproduced reproduits sans son autorisation.
  • Epidemiological Studies of Ovine Coccidial Infections in Selected Farms in Bauchi State, Nigeria

    Epidemiological Studies of Ovine Coccidial Infections in Selected Farms in Bauchi State, Nigeria

    EPIDEMIOLOGICAL STUDIES OF OVINE COCCIDIAL INFECTIONS IN SELECTED FARMS IN BAUCHI STATE, NIGERIA BY BIALLAH, MARKUS BUKAR A THESIS SUBMITTED TO THE POSTGRADUATE SCHOOL IN PARTIAL FULFILLMENT OF THE REQUIMENTS FOR THE AWARD OF MASTER OF SCIENCE IN VETERINARY PARASITOLOGY DEPARTMENT OF VETERINARY PARASITOLOGY AND ENTOMOLOGY FACULTY OF VETERINARY MEDICINE AHMADU BELLO UNIVERSITY ZARIA NIGERIA. SEPTEMBER, 2007 ii DECLARATION I declare that the work in the thesis entitled ‘EPIDEMIOLOGICAL STUDIES OF OVINE COCCIDIAL INFECTIONS IN SELECTED FARMS IN BAUCHI STATE, NIGERIA’ has been performed by me in the Department of Veterinary Parasitology and Entomology under the supervision of Dr. I. A. Lawal, Professors L. B. Tekdek and J.O Ajanusi. The information derived from the literature has been duly acknowledged in the text and a list of references provided. No part of this thesis was previously presented for another degree or diploma at any university. Biallah, Markus Bukar ____________________ _______________ Date iii CERTIFICATION This thesis entitled ‘EPIDEMIOLOGICAL STUDIES OF OVINE COCCIDIAL INFECTIONS IN SELECTED FARMS IN BAUCHI STATE, NIGERIA’ by Biallah, Markus Bukar meets to regulations governing the award of the degree of Master of Science of Ahmadu Bello University, Zaria, and is approved for its contribution to knowledge and literary presentation. ____________________________ _________________________ Dr. I. A. Lawal (DVM, M.Sc, Ph.D) Date Department of Veterinary Parasitology andEntomology, Ahmadu Bello University, Zaria. Chairman, Supervisory Committee ____________________________ _________________________ Prof. L. B. Tekdek (DVM, M.Sc, Ph.D) Date Department of Veterinary Surgery and Medicine Ahmadu Bello University, Zaria. Member, Supervisory Committee ____________________________ _________________________ Date Prof. J. O. Ajanusi (DVM, M.Sc, Ph.D) Department of Veterinary Parasitology and Entomology, Ahmadu Bello University, Zaria.
  • Eimeria Bovis Infections Induce G1 Cell Cycle Arrest and a Senescence-Like Phenotype in Cambridge.Org/Par Endothelial Host Cells

    Eimeria Bovis Infections Induce G1 Cell Cycle Arrest and a Senescence-Like Phenotype in Cambridge.Org/Par Endothelial Host Cells

    Parasitology Eimeria bovis infections induce G1 cell cycle arrest and a senescence-like phenotype in cambridge.org/par endothelial host cells 1 1,2 3 Research Article Zahady D. Velásquez , Sara López-Osorio , Daniel Waiger , Carolina Manosalva4, Learta Pervizaj-Oruqaj5,6,7, Susanne Herold5,6,7, Cite this article: Velásquez ZD, López-Osorio 1 1 S, Waiger D, Manosalva C, Pervizaj-Oruqaj L, Carlos Hermosilla and Anja Taubert Herold S, Hermosilla C, Taubert A (2021). 1 Eimeria bovis infections induce G1 cell cycle Institute of Parasitology, Biomedical Research Center Seltersberg, Justus Liebig University Giessen, Giessen, arrest and a senescence-like phenotype in Germany; 2Research Group CIVAB, School of Veterinary Medicine, Faculty of Agrarian Sciences, University of endothelial host cells. Parasitology 148, Antioquia, Medellin, Colombia; 3Center for Scientific Imaging, The Robert H. Smith Faculty of Agriculture, Food and 341–353. https://doi.org/10.1017/ Environment, Rehovot, Hebrew University of Jerusalem Israel, Rehovot, Israel; 4Faculty of Veterinary Sciences, S0031182020002097 Institute of Pharmacology, Universidad Austral de Chile, Valdivia, Chile; 5Cardio Pulmonary Institute (CPI), Giessen, 6 7 Received: 5 May 2020 Germany; Universities Giessen & Marburg Lung Center (UGMLC), Giessen, Germany and German Center for Lung Revised: 29 September 2020 Research (DZL), Giessen, Germany Accepted: 12 October 2020 First published online: 26 October 2020 Abstract Key words: Apicomplexan parasites are well-known to modulate their host cells at diverse functional Apicomplexan parasites; cell cycle arrest; levels. As such, apicomplexan-induced alteration of host cellular cell cycle was described Eimeria bovis; endothelial cell; nucleolar condensation; senescence and appeared dependent on both, parasite species and host cell type.
  • Eimeria Arloingi: Peculiarities of Its Life Cycle and Pathogenesis

    Eimeria Arloingi: Peculiarities of Its Life Cycle and Pathogenesis

    CARACTERIZAÇÃO DA INFECÇÃO POR EIMERIA SPP. EM CAPRINOS NO ALENTEJO TESE DE DOUTORAMENTO CHARACTERIZATION OF EIMERIA SPP INFECTIONS IN GOATS IN ALENTEJO Liliana Machado Ribeiro da Silva Tese apresentada à Universidade de Évora para obtenção do Grau de Doutor em Ciências Veterinárias ORIENTADOR (A/ES): Professor Helder Carola Espiguinha Cortes Professor Carlos Hermosilla Professora Anja Taubert ÉVORA, NOVEMBRO 2014 INSTITUTO DE INVESTIGAÇÃO E FORMAÇÃO AVANÇADA Aos meus Avós Ivete e Ircílio Machado por todo o apoio e amor que sempre me deram. “O sucesso é ter quem fique feliz com o meu sucesso, tão simples, e é mesmo assim, vale-me quem me abrace quando estou feliz, e no final das contas é mais por isso que estou feliz... todas as vitórias são colectivas, sobretudo as individuais.“ Pedro Chagas Freitas TABLE OF CONTENTS FIGURES ...................................................................................................................................... ii ABBREVIATIONS ......................................................................................................................... iii ABSTRACT .................................................................................................................................. iv RESUMO ...................................................................................................................................... v 1. INTRODUCTION ....................................................................................................................... 1 1.1. Social and economic
  • ESPÉCIES DO GÊNERO Eimeria SCHNEIDER, 1875, (APICOMPLEXA: EIMERIIDAE) PARASITOS DE CAPRINOS LEITEIROS NO MUNICÍPIO DE AFONSO BEZERRA, RIO GRANDE DO NORTE

    ESPÉCIES DO GÊNERO Eimeria SCHNEIDER, 1875, (APICOMPLEXA: EIMERIIDAE) PARASITOS DE CAPRINOS LEITEIROS NO MUNICÍPIO DE AFONSO BEZERRA, RIO GRANDE DO NORTE

    UNIVERSIDADE FEDERAL RURAL DO SEMI-ÁRIDO PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIA ANIMAL ESPÉCIES DO GÊNERO Eimeria SCHNEIDER, 1875, (APICOMPLEXA: EIMERIIDAE) PARASITOS DE CAPRINOS LEITEIROS NO MUNICÍPIO DE AFONSO BEZERRA, RIO GRANDE DO NORTE DANIELLY BEZERRA AVELINO Médica Veterinária MOSSORÓ –RN- BRASIL JUNHO – 2010 DANIELLY BEZERRA AVELINO ESPÉCIES DO GÊNERO Eimeria SCHNEIDER, 1875, (APICOMPLEXA: EIMERIIDAE) PARASITOS DE CAPRINOS LEITEIROS NO MUNICÍPIO DE AFONSO BEZERRA, RIO GRANDE DO NORTE Dissertação apresentada à Universidade Federal Rural do Semi-Árido - UFERSA, Campus de Mossoró, como parte das exigências para a obtenção do título de Mestre em Ciência Animal. Orientadora: Profª D. Sc. Sílvia Maria Mendes Ahid – UFERSA Co-orientador: Prof. D. Sc. Luiz da Silva Vieira – Embrapa Caprinos e Ovinos MOSSORÓ –RN- BRASIL JUNHO – 2010 Ficha catalográfica preparada pelo setor de classificação e catalogação da Biblioteca “Orlando Teixeira” da UFERSA A948e Avelino, Danielly Bezerra. Espécies do gênero Eimeria Schneider, 1875 (Apicomplexa: Eimeriidae) parasitos de caprinos leiteiros no município de Afonso Bezerra, Rio Grande do Norte / Danielly Bezerra Avelino. -- Mossoró, 2010. 51 f. Dissertação (Mestrado em Ciência Animal: Área de concentração em Parasitologia) – Universidade Federal Rural do Semi-Árido. Pró-Reitoria de Pós-Graduação. Orientadora: Profª. D. Sc. Sílvia Maria Mendes Ahid. Co-orientador: Profº. D. Sc. Luíz da Silva Vieira. 1. Caprino. 2. Eimeria . 3. Morfologia. 4. Frequência. I. Título. CDD: 636.089 691 Bibliotecário: Sale Mário Gaudêncio CRB-15/476 DANIELLY BEZERRA AVELINO ESPÉCIES DO GÊNERO Eimeria SCHNEIDER, 1875, (APICOMPLEXA: EIMERIIDAE) PARASITOS DE CAPRINOS LEITEIROS NO MUNICÍPIO DE AFONSO BEZERRA, RIO GRANDE DO NORTE Dissertação apresentada à Universidade Federal Rural do Semi-Árido - UFERSA, Campus de Mossoró, como parte das exigências para a obtenção do título de Mestre em Ciência Animal.