DOCSLIB.ORG

University of Groningen Gustatory Neural Processing in the Brainstem

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
University of Groningen Gustatory Neural Processing in the Brainstem University of Groningen Gustatory neural processing in the brainstem of the rat Streefland, Cerien IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Document Version Publisher's PDF, also known as Version of record Publication date: 1998 Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Streefland, C. (1998). Gustatory neural processing in the brainstem of the rat. s.n. Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). The publication may also be distributed here under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license. More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverne- amendment. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. Download date: 04-10-2021 CHAPTER 2 AUTONOMIC BRAINSTEM PROJECTIONS TO THE PANCREAS: A RETROGRADE TRANSNEURONAL VIRAL TRACING STUDY IN THE RAT Cerien Streefland, Frans W. Maes and Béla Bohus Groningen Graduate School for Behavioral and Cognitive Neurosciences (BCN), Department of Animal Physiology, University of Groningen, The Netherlands J.Auton.Nerv.Syst. (1998) (in press) 44 Chapter 2 ABSTRACT The present study describes brainstem nuclei that participate in the autonomic innervation of the pancreas, using a retrograde viral transneuronal tracing technique. It aimed at identifying the functional architecture of the parasympathetic, gustatory-induced insulin release by the endocrine pancreas (preabsorptive insulin response, PIR). Autonomic pathways organized for reflex adjustments of the end organ, as it happens in the PIR, involve relatively simple circuits. This implies a short brainstem circuit from the rostral gustatory nucleus of the solitary tract to the dorsal motor nucleus of the vagus. The present findings confirm projections to the pancreas, originating from preganglionic neurons in the dorsal motor nucleus of the vagus. Transneuronal labeling was detected in the medial, and to a lesser extent in the lateral nucleus of the solitary tract mainly at caudal and intermediate levels. Furthermore, infected neurons were seen in the brainstem in the dorsal and ventral part of the medullary reticular formation, in the area postrema and in the raphe nuclei. Sparse labeling was found in the gustatory zone of the nucleus tractus solitarius. These results indicate that a direct connection between the rostral nucleus tractus solitarius and the medial dorsal motor nucleus of the vagus is very unlikely, so that one or more intermediate stations may be involved. Candidates to complete this pathway are the intermediate or caudal nucleus tractus solitarius, the medullary reticular formation or the parabrachial nucleus. Autonomic brainstem projections to the pancreas 45 INTRODUCTION Autonomic pathways that control the endocrine pancreas are organized for both reflex adjustments of the pancreas and integrative functions involving more complex changes that affect multiple systems. Reflex pathways involve relatively simple brainstem circuits, while integration of ascending visceral information occurs in a variety of brainstem and forebrain nuclei, that command specific autonomic and neuroendocrine responses. The cephalic phase of insulin response PIR) is mediated through such a reflex pathway. In 1967 it was shown that electrical stimulation of vagal nerves projecting to the pancreas results in release of insulin 19. Since the efferent parasympathetic influences originate from the dorsal motor nucleus of the vagus (DMnX) in the brainstem 4,5,12,24,39, it is of interest to investigate the pathways afferent to this motor nucleus. The gustatory oropharyngeal influences that play a role in the causation of insulin release, and more specifically the functional architecture of this cephalic phase is of our long term interest 6,40. After oral intake of glucose by the rat, serum insulin levels rise within one minute, before the blood glucose level starts to increase 13,43. This preabsorptive insulin response is absent after vagotomy 5,35,36, but persists in decerebrated animals 11. The local circuitry of the brainstem therefore, seems to be sufficient to elicit the PIR. This suggests a short, direct pathway within the brainstem, from the gustatory input to the endocrine pancreas. Primary afferents from gustatory receptors terminate in the rostral part of the nucleus tractus solitarius (rNTS) 9,15,30,31. The aim of the present study is to trace the medullary input to the pancreatic preganglionics in the DMnX and more specifically, to elucidate connections from the gustatory region of the NTS to the DMnX. Although descending projections from the NTS have been reported 14,21,46, no detailed information is available concerning specific descending gustatory projection pathways. To demonstrate these projections, we chose to employ the rather novel retrograde transneuronal viral tracing technique 7,8,18,38,44. The major advantage of viral tracing is the visualization of functional chains of neurons. After virus injection into the pancreatic tissue, uptake by first-order (ganglionic) neurons innervating the pancreas takes place. Here, the viruses are replicated, which leads to amplifi- cation of the tracer signal. The viruses are then released by exocytosis into the extracellular space where they may specifically infect second-order (pregangli- onic) neurons 7. This process may then be repeated, infecting neurons presynaptic to the latter. 46 Chapter 2 MATERIALS AND METHODS All procedures used in this study were approved by the Committee on Animal Bioethics of the University of Groningen. Virus The virus used is the Bartha K strain of the Pseudorabies Virus (PrV) (Suid herpesvirus 1), an attenuated strain used to vaccinate pigs against Aujeszky's disease. The genome of Bartha PrV contains several mutations in genes encoding glycoproteins 27, decreasing the virulence of the virus. Plaqueing of the virus was carried out in green monkey kidney cells. Infection procedure Thirty-two male Wistar rats (190 - 210 g) were anesthetized with sodium pentobarbital (i.p., 30 mg/kg body wt.), hypnorm (i.m., 0.5 ml/kg, Janssen Pharmaceutica, NL) and atropine sulphate (i.p., 0.125 mg/kg). PrV was slowly injected (± 5 min) into the pancreatic tissue with a 30 G needle connected by a polythene tube (i.d. 0.26 mm) to a 1 ml syringe. The injections were placed in the 'head' of the pancreas, which has the highest content of insulin-secreting ß-cells 28. The peritoneum was closed and the rats were checked daily. Tissue preparation After 3 or 4 days, the rats were deeply anesthetized with sodium pentobarbital (i.p., 270 mg/kg) and perfused transcardially with 400 ml of fixative (4% paraformaldehyde in 0.1M phosphate buffer (PB), pH 7.4), preceded by a short prerinse with a 0.9 % heparinized saline solution. Brain and spinal cord were removed and postfixed for 2 hrs. The tissue was cryoprotected by overnight storage at 4 EC in 30 % sucrose in 0.1M PB. Transverse or longitudinal sections of brain and spinal cord were cut at 20-50 µm thickness on a cryostat microtome. The sections were collected in 0.1 M PB. Visualization of PrV Pseudorabies virus was visualized using an immunocytochemical peroxidase anti-peroxi- dase staining technique (PAP). Prior to the first antibody incubation, sections were rinsed in phosphate-buffered saline (PBS) and immersed for 10 minutes in 0.01 % H2O2 to exhaust endogenous peroxidase activity. The sections were rinsed again and incubated for 1 h at room temperature (RT) in 5% normal goat serum (NGS) to suppress non- specific antibody binding. Thereafter the sections were incubated overnight at RT in a primary antibody solution (1:2500, 0.1% triton X-100, 1% NGS). The rabbit-anti-PrV polyclonal antibody was kindly donated by Dr. J. Pol (Central Veterinary Institute, Lelystad, NL). After incubation the sections were thoroughly rinsed in PBS and again treated with 1% NGS for 1 h, followed by the secondary antibody incubation (goat-anti- rabbit IgG, 1:50; Zymed, USA) for 2 h at RT. The sections were rinsed again in PBS before the third incubation step (2 h at RT) in rabbit-PAP (1:500; Dakopatts, DK). Finally the sections were processed by the diaminobenzidine (DAB)-H2O2 reaction (30 mg DAB, 0.3% nickel ammoniumsulphate and 0.01 % H2O2/100 ml 0.1M Tris/HCl) After the immunocytochemical staining the sections were mounted, air dried, counterstained (with cresyl violet or neutral red/saffranine red), dehydrated, cleared in xylene and coverslipped with DPX mountant (BDH-Pool, UK). Autonomic brainstem projections to the pancreas 47 Experimental setup and control experiments Eleven rats received viral injections of 40 to 50 µl (1-1.5x104 plaque forming units (pfu's)) at 4 or 5 different locations in the ‘head’ of the pancreas. To check whether the injected volume of PrV spreads through the entire pancreas after the injection of PrV in the pancreatic ‘head’, 5 rats received a 1 µl injection at one location in the 'head' of the pancreas (with an identical amount of pfu's). Furthermore, 4 rats were given a 1 µl PrV injection in the ‘tail’ of the pancreas. In order to compare the innervation of these two pancreatic areas. To optimalize the infection (for criteria see: Analysis) 8 rats were used to vary the duration of the infection period (3 or 4 days) and the amount of pfu's injected (1.3x102 to 1.3x105 pfu's).
Load more

Recommended publications
  • An Illustrated Guide to Human Neuroematomy
    f:N-i,4I}TIA I APPENDIX INTRODUCTION SURFACEANATOMY OF THE BRAIN An Illustrated THE LATERALSURFACE OFTHE BRAIN /o) CrossFeotures Guide to Human (b) SelectedGyri,Sulci, ond Fissures Neuroematomy (c) CerebrolLobes ond the Insulo (d) Mojor Sensory,Motor, ond AssociotionAreos of Cortex THE MEDIALSURFACE OF THE BRAIN FT & (o) BroinStem Structures (b) ForebroinStructures (c) Ventricles THEVENTRALSURFACE OF THE BRAIN THE DORSALSURFACE OFTHE BRAIN (o) Cerebrum (b) CerebrumRernoved (c) Cerebrumond CerebellumRemoved CROSS.SECTIONALANATOMY OF THE BRAIN CROSSSECTION | : FOREBRAINAT THALAMUS-TELENCEPHALON JUNCTION (o) GrossFeotures (b) SelectedCell ond FiberGroups CROSSSECTION 2: FOREBRAINAT MID-THALAMUS (o) GrossFeotures (b) SelectedCell ond FiberGroups CROSSSECTION 3: FOREBRAINATTHALAMUS-MIDBRAIN JUNCTION (o) GrossFeotures (b) Se/eaedCell ond FiberGrouPs CROSSSECTION 4: ROSTRALMIDBRAIN CROSSSECTION 5: CAUDALMIDBRAIN CROSSSECTION 6: PONSAND CEREBELLUM CROSSSECTION 7: ROSTRALMEDULLA CROSSSECTION 8: MID-MEDULLA CROSSSECTION 9: MEDULLA-SPINALCORD JUNCTION THESPINAL CORD THE DORSALSURFACE OF THE SPINAL CORD AND SPINALNERVES THE VENTRAL-LATERAL SURFACE CROSS-SECTIONALANATOMY THEAUTONOMIC NERVOUS SYSTEM THECRANIAL NERVES THEBLOOD SUPPLY OF THE BRAIN VENTRALVIEW LATERALVIEW MEDTALVTEW(BRA|N STEM REMOVED) SELF.QUIZ j:' :: \) fi:; "i'- ,1,.., 206 C HAPTE R 7 . APPENDIX:ANILLUSTRATED GUIDETO HUMAN NEUROANATOMY W INTRODUCTION As we will see in the remainder of the book, a fruitful way to explore the nervous system is to divide it up into functional systems. Thus, the otfac- tlry systemconsists of those parts of the brain that are devoted to the sense of smell, the visual systemincludes those parts that are devoted to vision, and so on. while this functional approach to investigatingnervous sys- "big tem structure has many merits, it can make the picture,,-how all these systemsfit rogether inside the box we call the brain-difficult ro see.
    [Show full text]
  • Functional Organization of the Gustatory System in The
    Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 1986 Functional Organization of the Gustatory System in the Brains of Ictalurid Catfish: a Combined Electrophysiological and Neuroanatomical Study (Taste, Viscerotopic, Sensory Maps, Forebrain). Jagmeet Singh Kanwal Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Kanwal, Jagmeet Singh, "Functional Organization of the Gustatory System in the Brains of Ictalurid Catfish: a Combined Electrophysiological and Neuroanatomical Study (Taste, Viscerotopic, Sensory Maps, Forebrain)." (1986). LSU Historical Dissertations and Theses. 4244. https://digitalcommons.lsu.edu/gradschool_disstheses/4244 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. INFORMATION TO USERS This reproduction was made from a copy of a manuscript sent to us for publication and microfilming. While the most advanced technology has been used to pho­ tograph and reproduce this manuscript, the quality of the reproduction is heavily dependent upon the quality of the material submitted. Pages in any manuscript may have indistinct print. In all cases the best available copy has been filmed. The following explanation of techniques is provided to help clarify notations which may appear on this reproduction. 1. Manuscripts may not always be complete. When it is not possible to obtain missing pages, a note appears to indicate this. 2. When copyrighted materials are removed from the manuscript, a note ap­ pears to indicate this.
    [Show full text]
  • Functional Morphology of Gustatory Centers in the Brain of the Channel Catfish, Ictalurus Punctatus
    Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 1991 Functional Morphology of Gustatory Centers in the Brain of the Channel Catfish, Ictalurus Punctatus. Charles Franklin Lamb IV Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Lamb, Charles Franklin IV, "Functional Morphology of Gustatory Centers in the Brain of the Channel Catfish, Ictalurus Punctatus." (1991). LSU Historical Dissertations and Theses. 5254. https://digitalcommons.lsu.edu/gradschool_disstheses/5254 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand corner and continuing from left to right in equal sections with small overlaps.
    [Show full text]
  • 9.01 Introduction to Neuroscience Fall 2007
    MIT OpenCourseWare http://ocw.mit.edu 9.01 Introduction to Neuroscience Fall 2007 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms. Taste and smell Sebastian Seung Sensory transduction • How is the receptor potential generated? – ion channel – GPCR Psychology of taste • What is taste for? – Distinguish between food and poison – Distinguish between types of food • How many basic tastes are there? – salt, sour, sweet, bitter – umami Central taste pathways • Three cranial nerves from tongue • Medulla: gustatory nucleus – common pathway • Thalamocortical pathway – VPM – Gustatory cortex – Thought to be responsible for conscious perception Most gustatory axons respond to more than one basic taste • A distributed neural code 100 50 Action potentials / 5 sec 0 Sucrose NaCl HCl Quinine Figure by MIT OpenCourseWare. After Figure 8.4 in Bear, Mark F., Barry W. Connors, and Michael A. Paradiso. Neuroscience: Exploring the Brain. 3rd ed. Baltimore, MD: Lippincott Williams & Wilkins, 2007. Taste receptor cells • 50-150 in a taste bud • Synapses onto gustatory afferents Microvilli Taste pore Lingual epithelium Taste receptor cell Synapse Basal cell Connective tissue Gustatory afferent axons Figure by MIT OpenCourseWare. Taste receptors sweet T1R2+T1R3 umami T1R1+T1R3 GPCR bitter T2R (~30 types) sour PKD2L1 ion channel salt ? Genetic manipulations • Knockout – heterozygous – homozygous • Transgenic An alternate reality: labeled line encoding • Different tastes are cell type represented by the salt activation of nonoverlapping sets sour of neurons. • A single neuron can sweet unambiguously signal the presence of a taste. bitter salt sour sweet bitter stimulus Most receptor cells respond to more than one basic taste.
    [Show full text]
  • University of Groningen Gustatory Neural Processing in the Brainstem
    University of Groningen Gustatory neural processing in the brainstem of the rat Streefland, Cerien IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Document Version Publisher's PDF, also known as Version of record Publication date: 1998 Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Streefland, C. (1998). Gustatory neural processing in the brainstem of the rat. s.n. Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). The publication may also be distributed here under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license. More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverne- amendment. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. Download date: 02-10-2021 CHAPTER 1 General Introduction 14 Chapter 1 GENERAL INTRODUCTION to a study on gustatory neural processing in the brainstem of the rat 1.
    [Show full text]
  • INFORMATION to USERS the Quality of This Reproduction Is
    INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter 6ce, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book. Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6” x 9” black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. UMI A Bell & Howell Infonnadon Ccmpai^ 300 North Zeeb Road, Ann Arbor MI 48106-1346 USA 313/761-4700 800/521-0600 ORGANIZATION OF ORAL AND GASTRIC REPRESENTATIONS IN THE PARABRACHIAL NUCLEUS OF THE RAT DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Hamid Karimnamazi, D.D.S The Ohio State University 1998 Dissertation Committee: Professor Joseph Travers, PhD, Advisor Approved by Professor Susan Travers, PhD - Q 1- Advisor Professor Richard Rogers, PhD Oral Biology Graduate Program Professor Keith Alley, DDS, PhD Professor Laurel Fisher, PhD UMI Number: 9822327 UMI Microform 9822327 Copyright 1998, by UMI Company.
    [Show full text]
  • Parallel Medullary Gustatospinal Pathways in a Catfish: Possible
    The Journal of Neuroscience, June 15, 1997, 17(12):4873–4885 Parallel Medullary Gustatospinal Pathways In a Catfish: Possible Neural Substrates for Taste-Mediated Food Search Jagmeet S. Kanwal and Thomas E. Finger Department of Cellular and Structural Biology, University of Colorado School of Medicine, 4200 East Ninth Avenue, Denver, Colorado 80262 Taste and tactile fibers in the facial nerve of catfish innervate of HRP or fluorescent tracers into the medial lobule of the FL extraoral taste buds and terminate somatotopically in the facial label a facioreticular projection terminating around the Rsps of lobe (FL)—a medullary structure crucial for gustatory-mediated RS5. DiI injections into this area of the RF retrogradely label food search. The present study was performed to determine deeply situated bipolar neurons, especially in the medial and the neural linkages between the gustatory input and the spinal intermediate lobules of the FL. Electrophysiological recordings motor output. Spinal injections of horseradish peroxidase in and around RS5 show units with large receptive fields and (HRP) label spinopetal cells in the octaval nuclei, the nucleus of with responses to chemical and tactile stimulation. The FL the medial longitudinal fasciculus, and reticulospinal neurons projects to the spinal cord via two pathways: (1) a topograph- (Rsps) in the brainstem medial reticular formation (RF), includ- ically organized direct faciospinal pathway, and (2) an indirect ing the Mauthner cell. A somatotopically organized, direct fa- facioreticulospinal pathway in which reticular neurons process ciospinal system originating from superficial cells scattered in and integrate gustatory information before influencing spinal the lateral lobule of the facial lobe (ll) is also labeled.
    [Show full text]
  • Cranial Nerves and Their Nuclei
    CranialCranial nervesnerves andand theirtheir nucleinuclei 鄭海倫鄭海倫 整理整理 Cranial Nerves Figure 13.4a Location of the cranial nerves • Anterior cranial fossa: C.N. 1–2 • Middle cranial fossa: C.N. 3-6 • Posterior cranial fossa: C.N. 7-12 FunctionalFunctional componentscomponents inin nervesnerves • General Somatic Efferent • Special Visceral Afferent •GSE GSA GVE GVA • (SSE) SSA SVE SVA Neuron columns in the embryonic spinal cord * The floor of the 4th ventricle in the embryonic rhombencephalon Sp: special sensory B:branchial motor Ss: somatic sensory Sm: somataic motor Vi: visceral sensory A: preganglionic autonomic (visceral motor) • STT: spinothalamic tract • CST: corticospinal tract • ML: medial lemniscus Sensory nerve • Olfactory (1) •Optic (2) • Vestibulocochlear (8) Motor nerve • Oculomotor (3) • Trochlear (4) • Abducens (6) • Accessory (11) • Hypoglossal (12) Mixed nerve • Trigeminal (5) • Facial (7) • Glossopharyngeal (9) • Vagus (10) Innervation of branchial muscles • Trigemial • Facial • Glossopharyngeal • Vagus Cranial Nerve I: Olfactory Table 13.2(I) Cranial Nerve II: Optic • Arises from the retina of the eye • Optic nerves pass through the optic canals and converge at the optic chiasm • They continue to the thalamus (lateral geniculate body) where they synapse • From there, the optic radiation fibers run to the visual cortex (area 17) • Functions solely by carrying afferent impulses for vision Cranial Nerve II: Optic Table 13.2(II) Cranial Nerve III: Oculomotor • Fibers extend from the ventral midbrain, pass through the superior orbital fissure, and go to the extrinsic eye muscles • Functions in raising the eyelid, directing the eyeball, constricting the iris, and controlling lens shape Cranial Nerve III: Oculomotor Table 13.2(III) 1.Oculomotor nucleus (GSE) • Motor to ocular muscles: rectus (superior對側, inferior同側and medial同 側),inferior oblique同側, levator palpebrae superioris雙側 2.
    [Show full text]
  • Substance P Excites Neurons in the Gustatory Zone of the Rat Nucleus Tractus Solitarius
    120 Brain Research, 619 (1993) 1211-131) ,~) 1993 Elsevier Science Publishers B.V. All rights reserved 1)006-8993/93/$06.(li) BRES 19089 Substance P excites neurons in the gustatory zone of the rat nucleus tractus solitarius Michael S. King a, Limei Wang " and Robert M. Bradley a,b a Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078 (USA), b Department of Physiology, School of Medicine, University of Michigan, Ann Arbor, MI 48109-0622 (USA) (Accepted 9 March 1993) Key words: Brain slice; Electrophysiology; Morphology; Neurokinin; Sensory system; Taste; Whole-cell recording Whole-cell patch recordings of neurons in the rostral (gustatory) nucleus tractus solitarius (rNTS) were performed in a brain slice preparation from rat medulla. Neural responses to brief applications (10-45 s) of substance P (SP), via a constant superfusion apparatus, were recorded. SP transiently depolarized 80 of 117 (68%) rNTS neurons in a dose-dependent manner. Sub-micromolar concentrations of SP had potent excitatory effects, and the half maximal response occurred at 0.6 p.M. The depolarizing effect of SP was accompanied by an increase in input resistance in 81% of the responsive neurons. The excitatory effects of SP persisted in low Ca 2+ (0.2 mM) and high Mg z+ (12 mM) saline as well as in the presence of 2/xM TFX (n = 5 for each), suggesting direct postsynaptic action on the recorded neurons. SP also hyperpolarized 4 neurons (4%) and had no effect on 33 neurons (28%). Each of the 4 neurons which were hyperpolarized by SP showed a decrease in input resistance.
    [Show full text]
  • Taste Quality Representation in the Human Brain
    1042 • The Journal of Neuroscience, January 29, 2020 • 40(5):1042–1052 Systems/Circuits Taste Quality Representation in the Human Brain X Jason A. Avery, Alexander G. Liu, XJohn E. Ingeholm, Cameron D. Riddell, Stephen J. Gotts, and XAlex Martin Laboratory of Brain and Cognition, National Institute of Mental Health, Bethesda, Maryland 20892 In the mammalian brain, the insula is the primary cortical substrate involved in the perception of taste. Recent imaging studies in rodents have identified a “gustotopic” organization in the insula, whereby distinct insula regions are selectively responsive to one of the five basic tastes. However, numerous studies in monkeys have reported that gustatory cortical neurons are broadly-tuned to multiple tastes, and tastes are not represented in discrete spatial locations. Neuroimaging studies in humans have thus far been unable to discern between these two models, though this may be because of the relatively low spatial resolution used in taste studies to date. In the present study, we examined the spatial representation of taste within the human brain using ultra-high resolution functional magnetic resonance imaging (MRI) at high magnetic field strength (7-tesla). During scanning, male and female participants tasted sweet, salty, sour, and tasteless liquids, delivered via a custom-built MRI-compatible tastant-delivery system. Our univariate analyses revealed that all tastes (vs taste- less) activated primary taste cortex within the bilateral dorsal mid-insula, but no brain region exhibited a consistent preference for any individual taste. However, our multivariate searchlight analyses were able to reliably decode the identity of distinct tastes within those mid-insula regions, as well as brain regions involved in affect and reward, such as the striatum, orbitofrontal cortex, and amygdala.
    [Show full text]
  • Information Processing in the Rostral Solitary Nucleus: Modulation and Modeling
    Information Processing in the Rostral Solitary Nucleus: Modulation and Modeling DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Alison Boxwell Graduate Program in Neuroscience The Ohio State University 2015 Dissertation Committee: Dr. Joseph Travers, advisor Dr. Susan Travers Dr. David Terman Dr. Candice Askwith Copyright by Alison Boxwell 2015 Abstract The sense of taste contributes to quality of life and the vital function of discriminating between nutritive and toxic substances. It is intimately associated with appetitive and reward systems as well as homeostatic systems tasked with maintaining nutritional balance and body weight and is subject to widespread modulatory influences associated with digestive and satiety mechanisms including endogenous opioids. Central taste processing is initiated in the rostral nucleus of the solitary tract (rNST). Although there is a significant literature describing fundamental biophysical properties of rNST neurons, their inputs from the solitary tract (ST), and inhibition arising from local interneurons, it is not known how these properties contribute to shaping the gustatory signal as it passes from peripheral afferents to central rNST neurons. In addition, some properties which have been demonstrated in the rNST (such as convergence of ST afferents, short term synaptic depression (STSD) of ST-evoked currents, and inhibition) may interact in ways which are difficult to address using conventional experimental methods. Intrinsic properties and their interactions are central to understanding how the rNST acts to shape the gustatory signal as a relay to higher central structures and to local medullary reflex circuits. The work contained in this dissertation aims to determine the mechanism by which the activation of µ-opioid receptors modulates signal processing in the rNST, and finds that it is primarily a presynaptic inhibition of ST-evoked transmitter release.
    [Show full text]
  • Nucleus ...Nucleus
    A PHYLOGENETIC STUDY OF THE VISCERAL AF- FERENT AREAS ASSOCIATED WITH THE FACIAL, GLOSSOPHARYNGEAL, AND VAGUS NERVES, AND THEIR FIBER CONNECTIONS. THE EFFERENT FACIAL NUCLEUS JOHN WALTER BARNARD Laboratory of Comparative Neurology, Department of Anatomy, University of Niciichigan1 TWENTY-FOUR FIGURES (Accepted for publication September 21, 1935) CONTENTB Introduction ................ Description of the material .... The nuclear groups ........ 504 514 523 556 570 577 583 1. The visceral afferent gray 504 514 523 556 570 577 583 2. The inferior commissure 508 516 525 558 571 578 585 3. The secondary gustatory nucleus ............. 517 526 560 571 4. The efferentfacialnucleus 508 518 527 560 572 578 585 The root fibers ............ 509 518 528 538 561 572 579 586 1. The visceral afferent roots 509 518 528 538 561 572 579 586 2. The efferent facial root 510 519 529 541 563 573 580 587 The fiber connections of .... 511 520 530 542 564 573 581 587 1. The visceral afferent gray 511 520 530 542 564 573 581 587 2. The inferior commissure 513 521 535 550 567 575 582 590 3. The secondary gustatory nucleus ............. 522 536 551 568 575 4. The efferentfacialnucleus 513 523 538 555 568 577 583 590 Summary of description ...... 591 Discussion .................. 595 ’ A dissertation submitted in partial fulfillment of the requirements of the degree of doctor of philosophy. 503 504 JOHN WALTER BARNARD INTRODUCTION The following account deals with the phylogenetic develop- ment of the visceral afferent systems of the brain stem. The visceral afferent centers and their connections are considered in a series of vertebrates from cyclostomes through mam- mals.
    [Show full text]