SABiosciences PCR Array Catalog #: PAHS-021 SA+ SCF 4h stimulaton AVG(Ct) Position Unigene Refseq Symbol Description shContr shContr-4h shGSKβ A01 Hs.1274 NM_006129 BMP1 Bone morphogenetic protein 1 25.25 25.69 26.75 A02 Hs.73853 NM_001200 BMP2 Bone morphogenetic protein 2 36.25 34.87 36.11 A03 Hs.387411 NM_001201 BMP3 Bone morphogenetic protein 3 (osteogenic) A04 Hs.68879 NM_130851 BMP4 Bone morphogenetic protein 4 33.27 34.49 34.92 A05 Hs.296648 NM_021073 BMP5 Bone morphogenetic protein 5 A06 Hs.285671 NM_001718 BMP6 Bone morphogenetic protein 6 29.57 31.27 32.99 A07 Hs.473163 NM_001719 BMP7 Bone morphogenetic protein 7 (osteogenic protein 1) 36.23 A08 Hs.664022 NM_001720 BMP8B Bone morphogenetic protein 8b (osteogenic protein 2) 24.07 24.66 25.81 A09 Hs.591402 NM_000757 CSF1 Colony stimulating factor 1 (macrophage) 24.61 24.43 25.63 A10 Hs.1349 NM_000758 CSF2 Colony stimulating factor 2 (granulocyte-macrophage) 29.59 25.31 36.29 A11 Hs.473877 NM_058186 FAM3B Family with sequence similarity 3, member B 33.85 34.32 A12 Hs.2007 NM_000639 FASLG Fas ligand (TNF superfamily, member 6) 29.93 29.28 31.97 B01 Hs.11392 NM_004469 FIGF C-fos induced growth factor 31.92 32.64 33.51 B02 Hs.2171 NM_004962 GDF10 Growth differentiation factor 10 33.44 B03 Hs.643604 NM_005811 GDF11 Growth differentiation factor 11 23.90 23.63 26.18 B04 Hs.279463 NM_016204 GDF2 Growth differentiation factor 2 34.69 B05 Hs.86232 NM_020634 GDF3 Growth differentiation factor 3 24.96 25.43 29.32 B06 Hs.1573 NM_000557 GDF5 Growth differentiation factor 5 34.01 B07 Hs.41565 NM_005259 MSTN Myostatin 34.45 36.88 35.43 B08 Hs.25022 NM_005260 GDF9 Growth differentiation factor 9 29.51 30.68 31.83 B09 Hs.37026 NM_024013 IFNA1 Interferon, alpha 1 B10 Hs.211575 NM_000605 IFNA2 Interferon, alpha 2 37.45 B11 Hs.1510 NM_021068 IFNA4 Interferon, alpha 4 B12 Hs.37113 NM_002169 IFNA5 Interferon, alpha 5 35.16 38.61 C01 Hs.73890 NM_002170 IFNA8 Interferon, alpha 8 C02 Hs.93177 NM_002176 IFNB1 Interferon, beta 1, fibroblast 35.80 36.77 C03 Hs.856 NM_000619 IFNG Interferon, gamma 36.33 36.12 C04 Hs.591083 NM_020124 IFNK Interferon, kappa 36.21 33.74 35.81 C05 Hs.193717 NM_000572 IL10 Interleukin 10 20.49 21.32 25.36 C06 Hs.467304 NM_000641 IL11 Interleukin 11 C07 Hs.673 NM_000882 IL12A Interleukin 12A 28.68 29.04 30.40 C08 Hs.674 NM_002187 IL12B Interleukin 12B 38.02 C09 Hs.845 NM_002188 IL13 Interleukin 13 32.54 30.41 37.36 C10 Hs.699306 NM_175852 TXLNA Taxilin alpha 22.41 22.51 24.15 C11 Hs.654378 NM_000585 IL15 Interleukin 15 36.38 35.17 34.20 C12 Hs.459095 NM_004513 IL16 Interleukin 16 (lymphocyte chemoattractant factor) 23.55 24.10 25.60 D01 Hs.41724 NM_002190 IL17A Interleukin 17A D02 Hs.156979 NM_014443 IL17B Interleukin 17B 38.35 39.39 D03 Hs.278911 NM_013278 IL17C Interleukin 17C 32.08 32.40 34.22 D04 Hs.302036 NM_022789 IL25 Interleukin 25 D05 Hs.83077 NM_001562 IL18 Interleukin 18 (interferon-gamma-inducing factor) 20.69 21.38 23.58 D06 Hs.661017 NM_013371 IL19 Interleukin 19 27.09 26.92 29.47 D07 Hs.1722 NM_000575 IL1A Interleukin 1, alpha 24.26 24.91 29.53 D08 Hs.126256 NM_000576 IL1B Interleukin 1, beta 29.21 30.39 32.07 D09 Hs.306974 NM_173161 IL1F10 Interleukin 1 family, member 10 (theta) D10 Hs.516301 NM_012275 IL1F5 Interleukin 1 family, member 5 (delta) D11 Hs.278910 NM_014440 IL1F6 Interleukin 1 family, member 6 (epsilon) D12 Hs.166371 NM_173205 IL1F7 Interleukin 1 family, member 7 (zeta) 33.26 32.97 38.34 E01 Hs.278909 NM_173178 IL1F8 Interleukin 1 family, member 8 (eta) E02 Hs.211238 NM_019618 IL1F9 Interleukin 1 family, member 9 E03 Hs.89679 NM_000586 IL2 Interleukin 2 E04 Hs.272373 NM_018724 IL20 Interleukin 20 32.74 32.56 34.63 E05 Hs.567559 NM_021803 IL21 Interleukin 21 E06 Hs.287369 NM_020525 IL22 Interleukin 22 36.03 35.46 34.74 E07 Hs.411311 NM_006850 IL24 Interleukin 24 29.30 29.49 32.13 E08 Hs.694 NM_000588 IL3 Interleukin 3 (colony-stimulating factor, multiple) 35.04 30.69 E09 Hs.73917 NM_000589 IL4 Interleukin 4 33.37 35.13 36.57 E10 Hs.2247 NM_000879 IL5 Interleukin 5 (colony-stimulating factor, eosinophil) 33.28 32.50 34.28 E11 Hs.654458 NM_000600 IL6 Interleukin 6 (interferon, beta 2) 36.10 37.95 E12 Hs.591873 NM_000880 IL7 Interleukin 7 28.98 30.25 32.22 F01 Hs.624 NM_000584 IL8 Interleukin 8 29.35 27.13 33.85 F02 Hs.960 NM_000590 IL9 Interleukin 9 F03 Hs.407506 NM_002191 INHA Inhibin, alpha 35.82 F04 Hs.583348 NM_002192 INHBA Inhibin, beta A 30.09 29.86 30.82 F05 Hs.520187 NM_003240 LEFTY2 Left-right determination factor 2 37.36 36.90 37.43 F06 Hs.36 NM_000595 LTA Lymphotoxin alpha (TNF superfamily, member 1) 28.24 29.08 29.93 F07 Hs.376208 NM_002341 LTB Lymphotoxin beta (TNF superfamily, member 3) 27.39 28.92 29.29 F08 Hs.370414 NM_018055 NODAL Nodal homolog (mouse) 33.44 32.95 35.62 F09 Hs.707991 NM_002607 PDGFA Platelet-derived growth factor alpha polypeptide 23.95 23.83 25.47 F10 Hs.170009 NM_003236 TGFA Transforming growth factor, alpha 21.51 22.08 24.87 F11 Hs.645227 NM_000660 TGFB1 Transforming growth factor, beta 1 19.89 19.77 21.95 F12 Hs.133379 NM_003238 TGFB2 Transforming growth factor, beta 2 30.58 32.09 32.52 G01 Hs.592317 NM_003239 TGFB3 Transforming growth factor, beta 3 27.37 27.55 28.78 G02 Hs.241570 NM_000594 TNF Tumor necrosis factor (TNF superfamily, member 2) 26.94 27.66 28.63 G03 Hs.81791 NM_002546 TNFRSF11B Tumor necrosis factor receptor superfamily, member 11b G04 Hs.478275 NM_003810 TNFSF10 Tumor necrosis factor (ligand) superfamily, member 10 18.80 19.31 19.19 G05 Hs.333791 NM_003701 TNFSF11 Tumor necrosis factor (ligand) superfamily, member 11 29.71 30.01 34.45 G06 Hs.415839 NM_003809 TNFSF12 Tumor necrosis factor (ligand) superfamily, member 12 23.84 24.58 27.07 G07 Hs.54673 NM_003808 TNFSF13 Tumor necrosis factor (ligand) superfamily, member 13 26.26 27.03 28.66 G08 Hs.525157 NM_006573 TNFSF13B Tumor necrosis factor (ligand) superfamily, member 13b 29.68 30.28 32.57 G09 Hs.129708 NM_003807 TNFSF14 Tumor necrosis factor (ligand) superfamily, member 14 30.38 29.83 32.77 G10 Hs.181097 NM_003326 TNFSF4 Tumor necrosis factor (ligand) superfamily, member 4 24.88 26.31 30.07 G11 Hs.501497 NM_001252 CD70 CD70 molecule 30.79 31.06 31.97 G12 Hs.654445 NM_001244 TNFSF8 Tumor necrosis factor (ligand) superfamily, member 8 35.83 36.19 39.37 H01(HKG) Hs.534255 NM_004048 B2M Beta-2-microglobulin 15.87 16.11 17.75 H02(HKG) Hs.412707 NM_000194 HPRT1 Hypoxanthine phosphoribosyltransferase 1 21.22 21.69 24.44 H03(HKG) Hs.523185 NM_012423 RPL13A Ribosomal protein L13a 18.17 18.49 19.57 H04(HKG) Hs.544577 NM_002046 GAPDH Glyceraldehyde-3-phosphate dehydrogenase 16.85 17.21 18.60 H05(HKG) Hs.520640 NM_001101 ACTB Actin, beta 15.79 16.39 18.44 H06 N/A SA_00105 HGDC Human Genomic DNA Contamination H07 N/A SA_00104 RTC Reverse Transcription Control 21.63 22.04 22.33 H08 N/A SA_00104 RTC Reverse Transcription Control 21.58 22.01 22.38 H09 N/A SA_00104 RTC Reverse Transcription Control 21.63 22.00 22.52 H10 N/A SA_00103 PPC Positive PCR Control 18.11 18.46 18.39 H11 N/A SA_00103 PPC Positive PCR Control 18.52 18.88 18.85 H12 N/A SA_00103 PPC Positive PCR Control 18.28 18.49 18.43 SA+ SCF 4h stimulaton AVG Delta(Ct) Fold Change (Ct(sample) - Ave Ct(HKG)) (comparing to shCont w/o stimulation) shGSKβ-4h shContr shContr-4h shGSKβ shGSKβ-4h shContr shContr-4hshGSKβ shGSKβ-4h 27.73 7.67 7.71 6.99 6.99 1.00 0.97 1.60 1.61 38.14 17.42 16.89 15.24 14.26 1.00 1.44 4.54 8.94 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 33.33 15.69 16.51 15.16 12.59 1.00 0.56 1.44 8.57 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 34.64 11.99 13.29 13.23 13.90 1.00 0.41 0.42 0.27 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 26.77 6.49 6.68 6.05 6.03 1.00 0.88 1.36 1.38 26.54 7.03 6.45 5.87 5.80 1.00 1.49 2.24 2.34 32.94 12.01 7.33 15.24 12.20 1.00 25.63 0.11 0.87 16.27 16.34 15.24 14.26 1.00 0.95 2.04 4.02 33.35 12.35 11.30 12.21 12.61 1.00 2.07 1.10 0.83 33.85 14.34 14.66 13.75 13.11 1.00 0.80 1.50 2.34 17.42 15.46 15.24 14.26 1.00 3.89 4.54 8.94 27.10 6.32 5.65 6.41 6.36 1.00 1.59 0.93 0.97 17.11 17.02 15.24 14.26 1.00 1.06 3.65 7.20 30.89 7.38 7.45 9.56 10.14 1.00 0.95 0.22 0.15 16.43 17.02 15.24 14.26 1.00 0.67 2.29 4.51 37.03 16.87 17.02 15.24 14.26 1.00 0.90 3.09 6.09 32.41 11.93 12.70 12.07 11.67 1.00 0.59 0.91 1.20 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 39.10 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 17.42 15.76 15.24 14.26 1.00 3.17 4.54 8.94 27.42 2.91 3.34 5.60 6.68 1.00 0.74 0.16 0.07 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 31.96 11.10 11.06 10.63 11.21 1.00 1.03 1.38 0.92 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 36.69 14.96 12.43 15.24 14.26 1.00 5.75 0.82 1.62 24.72 4.83 4.53 4.39 3.98 1.00 1.23 1.36 1.81 17.42 17.02 14.44 14.26 1.00 1.32 7.87 8.94 26.02 5.97 6.12 5.84 5.28 1.00 0.90 1.10 1.62 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 35.57 14.50 14.42 14.46 14.26 1.00 1.06 1.03 1.18 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 24.79 3.11 3.40 3.82 4.05 1.00 0.82 0.61 0.52 30.24 9.51 8.94 9.70 9.50 1.00 1.49 0.87 1.01 31.26 6.68 6.93 9.77 10.52 1.00 0.84 0.12 0.07 35.90 11.63 12.41 12.31 14.26 1.00 0.58 0.63 0.16 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 34.00 15.68 14.99 15.24 13.26 1.00 1.61 1.35 5.33 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 15.16 14.58 14.87 14.26 1.00 1.49 1.22 1.87 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 36.40 17.42 17.02 14.98 14.26 1.00 1.32 5.43 8.94 32.46 11.72 11.51 12.36 11.72 1.00 1.16 0.64 1.00 17.42 12.71 15.24 14.26 1.00 26.10 4.54 8.94 15.79 17.02 15.24 14.26 1.00 0.43 1.47 2.90 39.15 15.70 14.52 14.52 14.26 1.00 2.25 2.27 2.71 36.11 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 34.08 11.39 12.27 12.46 13.34 1.00 0.54 0.48 0.26 32.94 11.77 9.15 14.09 12.20 1.00 6.13 0.20 0.74 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 34.63 17.42 17.02 15.24 13.89 1.00 1.32 4.54 11.53 32.15 12.50 11.88 11.06 11.41 1.00 1.54 2.73 2.14 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 30.05 10.66 11.11 10.17 9.30 1.00 0.73 1.40 2.55 30.01 9.81 10.94 9.53 9.27 1.00 0.46 1.22 1.46 37.41 15.86 14.97 15.24 14.26 1.00 1.86 1.54 3.04 25.42 6.37 5.85 5.71 4.68 1.00 1.44 1.59 3.23 25.23 3.93 4.10 5.11 4.49 1.00 0.89 0.44 0.68 22.01 2.31 1.79 2.19 1.27 1.00 1.43 1.09 2.06 32.84 13.00 14.11 12.76 12.10 1.00 0.46 1.18 1.86 29.05 9.79 9.57 9.02 8.31 1.00 1.16 1.71 2.79 29.29 9.36 9.68 8.87 8.55 1.00 0.81 1.41 1.76 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 19.66 1.22 1.33 -0.57 -1.08 1.00 0.93 3.47 4.94 37.05 12.13 12.03 14.69 14.26 1.00 1.07 0.17 0.23 27.86 6.26 6.60 7.31 7.12 1.00 0.79 0.48 0.55 29.67 8.68 9.05 8.90 8.92 1.00 0.77 0.86 0.84 34.03 12.10 12.30 12.81 13.29 1.00 0.87 0.61 0.44 32.14 12.80 11.85 13.01 11.39 1.00 1.93 0.87 2.65 31.94 7.30 8.33 10.30 11.20 1.00 0.49 0.12 0.07 32.06 13.21 13.08 12.21 11.32 1.00 1.10 2.00 3.72 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 18.55 -1.71 -1.87 -2.01 -2.19 1.00 1.11 1.23 1.40 25.67 3.64 3.71 4.68 4.93 1.00 0.95 0.49 0.41 20.13 0.59 0.51 -0.19 -0.61 1.00 1.06 1.71 2.30 19.28 -0.73 -0.77 -1.17 -1.46 1.00 1.02 1.35 1.66 20.08 -1.79 -1.59 -1.32 -0.66 1.00 0.87 0.72 0.46 17.42 17.02 15.24 14.26 1.00 1.32 4.54 8.94 22.57 4.05 4.06 2.57 1.83 1.00 1.00 2.80 4.68 22.62 4.00 4.03 2.62 1.88 1.00 0.98 2.61 4.37 22.60 4.05 4.02 2.76 1.86 1.00 1.02 2.45 4.55 19.26 0.53 0.48 -1.37 -1.48 1.00 1.03 3.73 4.03 19.71 0.94 0.90 -0.91 -1.03 1.00 1.02 3.60 3.91 18.22 0.70 0.51 -1.33 -2.52 1.00 1.15 4.10 9.38