CD200 Upregulation in Vascular Endothelium Surrounding Cutaneous Squamous Cell Carcinoma

Supplementary Online Content

Belkin DA, Mitsui H, Wang CQ, et al. CD200 upregulation in vascular endothelium surrounding cutaneous squamous cell carcinoma. Arch Dermatol. Published online December 17, 2012. doi:10.1001/jamadermatol.2013.1609

eTable 1. Antibodies used in immunohistochemistry and immunofluorescence eTable 2. Full list of up- and downregulated genes in SCC stroma compared with normal reticular dermis. eTable 3. Endotoxin assay of tumor supernatants

This supplementary material has been provided by the authors to give readers additional information about their work.

Downloaded From: https://jamanetwork.com/ on 10/01/2021 eTable 1. Antibodies used in immunohistochemistry and immunofluorescence

Antigen Manufacturer Clonea Isotype Dilution Detectionb CD3-FITC BD Biosciences SK7 IgG1 1:100 Goat anti-FITC- A488 CD11c BD Pharmingen B-ly6 IgG1 1:100 Zenon-labelingc CD31 BD Pharmingen WM59 IgG1 1:100 Zenon-labeling CD163-FITC Acris 5C6- IgG1 1:100 Goat anti-FITC- FAT A488 CD200 Abcam MRC IgG1 1:100 Goat anti-mouse OX104 IgG1-A488 CD200R BioLegend OX-108 IgG1 1:50 Goat anti-mouse IgG1-A568d CD207 Abcam 12D6 IgG2b 1:100 Goat anti-mouse IgG2b-A488 NKp46 R&D Systems 195314 IgG2b 1:50 Goat anti-mouse IgG2b-A488 a. All antibodies were murine monoclonal b. For immunofluorescence only c. According to the manufacturer’s instructions (Invitrogen) d. Except against CD11c‐zenon, where goat anti‐mouse IgG1‐A488 was used

Downloaded From: https://jamanetwork.com/ on 10/01/2021 eTable 2. Full list of up- and downregulated genes in SCC stroma compared with normal reticular dermis. FCH > 4.0, FDR < 0.05. Probe Symbol Description FCH P FDR 205242_at CXCL13 chemokine (C-X-C motif) ligand 13 135.361 0 0 202917_s_at S100A8 S100 calcium binding protein A8 93.074 0 0 203915_at CXCL9 chemokine (C-X-C motif) ligand 9 57.513 0 0 203535_at S100A9 S100 calcium binding protein A9 45.934 0 0

32128_at CCL18 chemokine (C-C motif) ligand 18 (pulmonary 40.398 0 0 and activation-regulated) 204533_at CXCL10 chemokine (C-X-C motif) ligand 10 33.817 0 0 214580_x_at 33.637 0 0 209125_at KRT6A keratin 6A 26.137 0 0 207861_at CCL22 chemokine (C-C motif) ligand 22 25.487 0 0 204475_at MMP1 matrix metallopeptidase 1 (interstitial 24.966 0.001 0 collagenase) 204563_at SELL selectin L 24.89 0 0 211430_s_at 24.362 0 0 221969_at 23.713 0 0 213680_at KRT6B keratin 6B 21.829 0 0 210164_at GZMB granzyme B (granzyme 2, cytotoxic T- 21.722 0 0 lymphocyte-associated serine esterase 1) 209395_at CHI3L1 chitinase 3-like 1 (cartilage glycoprotein-39) 21.681 0 0 205943_at TDO2 tryptophan 2,3-dioxygenase 20.282 0 0 210029_at IDO1 indoleamine 2,3-dioxygenase 1 19.135 0 0 215504_x_at 18.508 0 0 62987_r_at CACNG4 calcium channel, voltage-dependent, gamma 18.416 0 0 subunit 4 209924_at CCL18 chemokine (C-C motif) ligand 18 (pulmonary 16.554 0 0 and activation-regulated) 204268_at S100A2 S100 calcium binding protein A2 16.444 0 0 216745_x_at 16.426 0 0 206134_at ADAMDEC1 ADAM-like, decysin 1 15.854 0 0 212236_x_at KRT17 keratin 17 15.089 0 0 206337_at CCR7 chemokine (C-C motif) receptor 7 14.649 0 0 203936_s_at MMP9 matrix metallopeptidase 9 (gelatinase B, 92kDa 14.468 0 0 gelatinase, 92kDa type IV collagenase) 209875_s_at SPP1 secreted phosphoprotein 1 14.378 0 0 216834_at RGS1 regulator of G-protein signaling 1 14.176 0 0 203435_s_at MME membrane metallo-endopeptidase 14.038 0 0 219836_at ZBED2 zinc finger, BED-type containing 2 13.422 0 0 213790_at ADAM12 ADAM metallopeptidase domain 12 13.241 0 0 212942_s_at KIAA1199 KIAA1199 13.171 0 0 206974_at CXCR6 chemokine (C-X-C motif) receptor 6 12.966 0 0 205916_at S100A7 S100 calcium binding protein A7 12.608 0 0 204949_at ICAM3 intercellular adhesion molecule 3 12.501 0 0 214032_at ZAP70 zeta-chain (TCR) associated protein kinase 12.42 0 0 70kDa 219191_s_at BIN2 bridging integrator 2 11.286 0 0 203878_s_at MMP11 matrix metallopeptidase 11 (stromelysin 3) 11.239 0 0 210448_s_at P2RX5 purinergic receptor P2X, ligand-gated ion 11.063 0 0 channel, 5 204580_at MMP12 matrix metallopeptidase 12 (macrophage 10.777 0.001 0 elastase) 214038_at CCL8 chemokine (C-C motif) ligand 8 10.744 0 0 31846_at RHOD ras homolog gene family, member D 10.299 0 0 204769_s_at TAP2 transporter 2, ATP-binding cassette, sub-family 10.254 0 0 B (MDR/TAP)

Downloaded From: https://jamanetwork.com/ on 10/01/2021 217619_x_at 10.079 0 0 204416_x_at APOC1 apolipoprotein C-I 9.929 0 0 204960_at PTPRCAP protein tyrosine phosphatase, receptor type, C- 9.904 0 0 associated protein 37892_at COL11A1 collagen, type XI, alpha 1 9.765 0 0 209959_at NR4A3 nuclear receptor subfamily 4, group A, member 3 9.707 0 0 202988_s_at RGS1 regulator of G-protein signaling 1 9.689 0 0 209396_s_at CHI3L1 chitinase 3-like 1 (cartilage glycoprotein-39) 9.523 0 0 38447_at ADRBK1 adrenergic, beta, receptor kinase 1 9.418 0 0 205119_s_at FPR1 formyl peptide receptor 1 9.38 0 0 205798_at IL7R interleukin 7 receptor 9.223 0 0 211269_s_at IL2RA interleukin 2 receptor, alpha 9.155 0 0 35150_at CD40 CD40 molecule, TNF receptor superfamily 9.134 0 0 member 5 209773_s_at RRM2 ribonucleotide reductase M2 8.886 0 0 38707_r_at E2F4 E2F transcription factor 4, p107/p130-binding 8.768 0 0 218745_x_at TMEM161A transmembrane protein 161A 8.707 0 0 205599_at TRAF1 TNF receptor-associated factor 1 8.644 0 0 90610_at LRCH4 leucine-rich repeats and calponin homology 8.596 0 0 (CH) domain containing 4 179_at PMS2L11 postmeiotic segregation increased 2-like 11 8.272 0 0 pseudogene 208851_s_at THY1 Thy-1 cell surface antigen 8.252 0 0 210439_at ICOS inducible T-cell co-stimulator 8.203 0 0 219946_x_at MYH14 myosin, heavy chain 14 8.098 0 0 213909_at LRRC15 leucine rich repeat containing 15 8.059 0 0 205569_at LAMP3 lysosomal-associated membrane protein 3 8.028 0 0 206298_at ARHGAP22 Rho GTPase activating protein 22 7.979 0 0 204714_s_at F5 coagulation factor V (proaccelerin, labile factor) 7.957 0 0 207351_s_at SH2D2A SH2 domain protein 2A 7.901 0 0 205114_s_at 7.879 0 0 204015_s_at DUSP4 dual specificity phosphatase 4 7.875 0 0 206569_at IL24 interleukin 24 7.684 0 0 221081_s_at DENND2D DENN/MADD domain containing 2D 7.637 0 0 39854_r_at PNPLA2 patatin-like phospholipase domain containing 2 7.587 0 0 214391_x_at PTGER1 prostaglandin E receptor 1 (subtype EP1), 42kDa 7.575 0 0 204794_at DUSP2 dual specificity phosphatase 2 7.558 0 0 203699_s_at DIO2 deiodinase, iodothyronine, type II 7.545 0 0 204103_at CCL4 chemokine (C-C motif) ligand 4 7.494 0 0 215978_x_at LOC152719 hypothetical protein LOC152719 7.484 0 0 205405_at SEMA5A sema domain, seven thrombospondin repeats 7.459 0 0 (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A 221601_s_at FAIM3 Fas apoptotic inhibitory molecule 3 7.445 0 0 218822_s_at 7.418 0 0 202284_s_at CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) 7.368 0 0 31861_at IGHMBP2 immunoglobulin mu binding protein 2 7.346 0 0 201402_at ADRBK1 adrenergic, beta, receptor kinase 1 7.291 0 0 202953_at C1QB complement component 1, q subcomponent, B 7.254 0 0 chain 214370_at S100A8 S100 calcium binding protein A8 7.25 0 0 209324_s_at RGS16 regulator of G-protein signaling 16 7.225 0 0 56829_at TRAPPC9 trafficking protein particle complex 9 7.186 0 0 221658_s_at IL21R interleukin 21 receptor 7.171 0 0 209270_at LAMB3 laminin, beta 3 7.11 0 0 204961_s_at 7.098 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 36004_at IKBKG inhibitor of kappa light polypeptide gene 7 0 0 enhancer in B-cells, kinase gamma 221690_s_at NLRP2 NLR family, pyrin domain containing 2 6.985 0 0 205205_at RELB v-rel reticuloendotheliosis viral oncogene 6.968 0 0 homolog B 205479_s_at PLAU plasminogen activator, urokinase 6.936 0 0 205267_at POU2AF1 POU class 2 associating factor 1 6.894 0 0 203559_s_at ABP1 amiloride binding protein 1 (amine oxidase 6.881 0 0 (copper-containing)) 48030_i_at C5orf4 chromosome 5 open reading frame 4 6.875 0 0 205157_s_at KRT17 keratin 17 6.841 0.002 0 206513_at AIM2 absent in melanoma 2 6.797 0 0 209126_x_at KRT6B keratin 6B 6.701 0 0 209138_x_at IGL@ immunoglobulin lambda locus 6.68 0.004 0.01 205270_s_at LCP2 lymphocyte cytosolic protein 2 (SH2 domain 6.647 0 0 containing leukocyte protein of 76kDa) 39402_at IL1B interleukin 1, beta 6.562 0 0 206214_at PLA2G7 phospholipase A2, group VII (platelet-activating 6.509 0 0 factor acetylhydrolase, plasma) 203610_s_at TRIM38 tripartite motif-containing 38 6.475 0 0 209870_s_at APBA2 amyloid beta (A4) precursor protein-binding, 6.468 0 0 family A, member 2 33304_at ISG20 interferon stimulated exonuclease gene 20kDa 6.459 0 0 207681_at CXCR3 chemokine (C-X-C motif) receptor 3 6.406 0 0 49327_at SIRT3 sirtuin (silent mating type information regulation 6.354 0 0 2 homolog) 3 (S. cerevisiae) 203052_at C2 complement component 2 6.337 0 0 215865_at SYT12 synaptotagmin XII 6.323 0 0 205965_at BATF basic leucine zipper transcription factor, ATF- 6.303 0 0 like 219014_at PLAC8 placenta-specific 8 6.256 0 0 220203_at BMP8A bone morphogenetic protein 8a 6.228 0 0 205666_at FMO1 flavin containing monooxygenase 1 6.221 0 0 204951_at RHOH ras homolog gene family, member H 6.218 0 0 207253_s_at UBN1 ubinuclein 1 6.202 0 0 218834_s_at TMEM132A transmembrane protein 132A 6.158 0 0 213915_at NKG7 natural killer cell group 7 sequence 6.15 0 0 208850_s_at THY1 Thy-1 cell surface antigen 6.126 0 0 214081_at PLXDC1 plexin domain containing 1 6.108 0 0 218810_at ZC3H12A zinc finger CCCH-type containing 12A 6.089 0 0 211178_s_at PSTPIP1 proline-serine-threonine phosphatase interacting 6.036 0 0 protein 1 204698_at ISG20 interferon stimulated exonuclease gene 20kDa 5.996 0 0 213442_x_at SPDEF SAM pointed domain containing ets 5.973 0 0 transcription factor 216950_s_at 5.969 0 0 220042_x_at HIVEP3 human immunodeficiency virus type I enhancer 5.957 0 0 binding protein 3 211336_x_at LILRB1 leukocyte immunoglobulin-like receptor, 5.905 0 0 subfamily B (with TM and ITIM domains), member 1 205291_at IL2RB interleukin 2 receptor, beta 5.901 0 0 209671_x_at 5.88 0 0 214084_x_at NCF1C neutrophil cytosolic factor 1C pseudogene 5.87 0 0 210915_x_at 5.867 0 0 208583_x_at HIST1H2AJ histone cluster 1, H2aj 5.849 0 0 219684_at RTP4 receptor (chemosensory) transporter protein 4 5.846 0 0 205402_x_at PRSS2 protease, serine, 2 (trypsin 2) 5.844 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 221210_s_at NPL N-acetylneuraminate pyruvate lyase 5.836 0 0 (dihydrodipicolinate synthase) 201422_at IFI30 interferon, gamma-inducible protein 30 5.814 0 0 204858_s_at TYMP thymidine phosphorylase 5.774 0 0 213170_at GPX7 glutathione peroxidase 7 5.745 0 0 203470_s_at PLEK pleckstrin 5.659 0 0 208260_at AVPR1B arginine vasopressin receptor 1B 5.648 0 0 206165_s_at CLCA2 chloride channel accessory 2 5.63 0 0 213947_s_at NUP210 nucleoporin 210kDa 5.63 0 0 209747_at TGFB3 transforming growth factor, beta 3 5.62 0 0 211282_x_at TNFRSF25 tumor necrosis factor receptor superfamily, 5.6 0 0 member 25 214511_x_at FCGR1B Fc fragment of IgG, high affinity Ib, receptor 5.59 0 0 (CD64) 202998_s_at LOXL2 lysyl oxidase-like 2 5.589 0 0 121_at PAX8 paired box 8 5.582 0 0 203691_at PI3 peptidase inhibitor 3, skin-derived 5.562 0.003 0 204316_at RGS10 regulator of G-protein signaling 10 5.561 0 0 213193_x_at 5.56 0 0 209051_s_at RALGDS ral guanine nucleotide dissociation stimulator 5.559 0 0 219554_at RHCG Rh family, C glycoprotein 5.557 0 0 212827_at IGHM immunoglobulin heavy constant mu 5.547 0 0 202800_at SLC1A3 solute carrier family 1 (glial high affinity 5.543 0 0 glutamate transporter), member 3 205988_at CD84 CD84 molecule 5.536 0 0 210644_s_at LAIR1 leukocyte-associated immunoglobulin-like 5.525 0 0 receptor 1 55705_at C19orf22 chromosome 19 open reading frame 22 5.525 0 0 219781_s_at ZNF771 zinc finger protein 771 5.504 0 0 205825_at PCSK1 proprotein convertase subtilisin/kexin type 1 5.497 0 0 207339_s_at LTB lymphotoxin beta (TNF superfamily, member 3) 5.495 0 0 205067_at IL1B interleukin 1, beta 5.443 0 0 203554_x_at PTTG1 pituitary tumor-transforming 1 5.43 0 0 204320_at COL11A1 collagen, type XI, alpha 1 5.414 0 0 210356_x_at MS4A1 membrane-spanning 4-domains, subfamily A, 5.404 0 0 member 1 213869_x_at THY1 Thy-1 cell surface antigen 5.369 0 0 37145_at GNLY granulysin 5.368 0 0 219852_s_at MORN1 MORN repeat containing 1 5.359 0 0 209457_at DUSP5 dual specificity phosphatase 5 5.358 0 0 213416_at ITGA4 integrin, alpha 4 (antigen CD49D, alpha 4 5.343 0 0 subunit of VLA-4 receptor) 207072_at IL18RAP interleukin 18 receptor accessory protein 5.316 0 0 210163_at CXCL11 chemokine (C-X-C motif) ligand 11 5.307 0.005 0.01 202628_s_at SERPINE1 serpin peptidase inhibitor, clade E (nexin, 5.302 0 0 plasminogen activator inhibitor type 1), member 1 221080_s_at DENND1C DENN/MADD domain containing 1C 5.296 0 0 203922_s_at CYBB cytochrome b-245, beta polypeptide 5.268 0 0 53912_at SNX11 sorting nexin 11 5.262 0 0 207509_s_at LAIR2 leukocyte-associated immunoglobulin-like 5.257 0 0 receptor 2 204952_at LYPD3 LY6/PLAUR domain containing 3 5.23 0 0 209929_s_at IKBKG inhibitor of kappa light polypeptide gene 5.23 0 0 enhancer in B-cells, kinase gamma 210809_s_at POSTN periostin, osteoblast specific factor 5.153 0 0 210347_s_at BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) 5.139 0 0 211552_s_at ALDH4A1 aldehyde dehydrogenase 4 family, member A1 5.135 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 210313_at LILRA4 leukocyte immunoglobulin-like receptor, 5.127 0 0 subfamily A (with TM domain), member 4 203028_s_at CYBA cytochrome b-245, alpha polypeptide 5.107 0 0 214941_s_at PRPF40A PRP40 pre-mRNA processing factor 40 homolog 5.1 0 0 A (S. cerevisiae) 204007_at FCGR3B Fc fragment of IgG, low affinity IIIb, receptor 5.076 0 0 (CD16b) 33197_at MYO7A myosin VIIA 5.073 0 0 202458_at PRSS23 protease, serine, 23 5.06 0 0 39318_at TCL1A T-cell leukemia/lymphoma 1A 5.048 0 0 202523_s_at SPOCK2 sparc/osteonectin, cwcv and kazal-like domains 5.045 0 0 proteoglycan (testican) 2 220335_x_at CES3 carboxylesterase 3 5.035 0 0 213553_x_at APOC1 apolipoprotein C-I 5.01 0 0 207035_at SLC30A3 solute carrier family 30 (zinc transporter), 4.997 0 0 member 3 204385_at KYNU kynureninase (L-kynurenine hydrolase) 4.995 0 0 218382_s_at U2AF2 U2 small nuclear RNA auxiliary factor 2 4.976 0 0 205671_s_at HLA-DOB major histocompatibility complex, class II, DO 4.974 0 0 beta 201473_at JUNB jun B proto-oncogene 4.952 0 0 202253_s_at DNM2 dynamin 2 4.934 0 0 44617_at OGFOD2 2-oxoglutarate and iron-dependent oxygenase 4.934 0 0 domain containing 2 211796_s_at 4.929 0 0 91920_at BCAN brevican 4.924 0 0 205890_s_at 4.921 0 0 219622_at RAB20 RAB20, member RAS oncogene family 4.909 0 0 216495_x_at IVD isovaleryl Coenzyme A dehydrogenase 4.9 0 0 209583_s_at CD200 CD200 molecule 4.894 0 0 221521_s_at GINS2 GINS complex subunit 2 (Psf2 homolog) 4.887 0 0 213060_s_at CHI3L2 chitinase 3-like 2 4.868 0 0 41657_at STK11 serine/threonine kinase 11 4.85 0 0 211919_s_at CXCR4 chemokine (C-X-C motif) receptor 4 4.836 0 0 210511_s_at INHBA inhibin, beta A 4.829 0 0 209374_s_at IGHM immunoglobulin heavy constant mu 4.819 0.01 0.02 40489_at ATN1 atrophin 1 4.813 0 0 202531_at IRF1 interferon regulatory factor 1 4.784 0 0 217418_x_at MS4A1 membrane-spanning 4-domains, subfamily A, 4.764 0 0 member 1 204189_at RARG retinoic acid receptor, gamma 4.76 0 0 218837_s_at UBE2D4 ubiquitin-conjugating enzyme E2D 4 (putative) 4.714 0 0 219344_at SLC29A3 solute carrier family 29 (nucleoside transporters), 4.709 0 0 member 3 204470_at CXCL1 chemokine (C-X-C motif) ligand 1 (melanoma 4.708 0 0 growth stimulating activity, alpha) 204218_at C11orf51 chromosome 11 open reading frame 51 4.702 0 0 222017_x_at LRCH4 leucine-rich repeats and calponin homology 4.699 0 0 (CH) domain containing 4 206666_at GZMK granzyme K (granzyme 3; tryptase II) 4.698 0 0 205946_at VIPR2 vasoactive intestinal peptide receptor 2 4.687 0 0 204197_s_at RUNX3 runt-related transcription factor 3 4.675 0 0 215429_s_at ZNF428 zinc finger protein 428 4.663 0 0 214228_x_at TNFRSF4 tumor necrosis factor receptor superfamily, 4.652 0 0 member 4 203490_at ELF4 E74-like factor 4 (ets domain transcription 4.646 0 0 factor) 209800_at KRT16 keratin 16 4.64 0.003 0 64440_at IL17RC interleukin 17 receptor C 4.637 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 218299_at C11orf24 chromosome 11 open reading frame 24 4.635 0 0 200615_s_at AP2B1 adaptor-related protein complex 2, beta 1 subunit 4.63 0 0 219281_at MSRA methionine sulfoxide reductase A 4.628 0 0 219716_at APOL6 apolipoprotein L, 6 4.627 0 0 213441_x_at SPDEF SAM pointed domain containing ets 4.624 0 0 transcription factor 219593_at SLC15A3 solute carrier family 15, member 3 4.624 0 0 38710_at OTUB1 OTU domain, ubiquitin aldehyde binding 1 4.621 0 0 203971_at SLC31A1 solute carrier family 31 (copper transporters), 4.613 0 0 member 1 216499_at 4.612 0 0 206231_at KCNN1 potassium intermediate/small conductance 4.606 0 0 calcium-activated channel, subfamily N, member 1 202524_s_at SPOCK2 sparc/osteonectin, cwcv and kazal-like domains 4.595 0 0 proteoglycan (testican) 2 202068_s_at LDLR low density lipoprotein receptor 4.58 0 0 211745_x_at 4.579 0.005 0.01 215121_x_at IGL@ immunoglobulin lambda locus 4.579 0.009 0.02 206219_s_at VAV1 vav 1 guanine nucleotide exchange factor 4.567 0 0 213230_at CDR2L cerebellar degeneration-related protein 2-like 4.544 0 0 212235_at PLXND1 plexin D1 4.532 0 0 220418_at UBASH3A ubiquitin associated and SH3 domain containing, 4.53 0 0 A 216945_x_at PASK PAS domain containing serine/threonine kinase 4.519 0 0 203580_s_at SLC7A6 solute carrier family 7 (cationic amino acid 4.508 0 0 transporter, y+ system), member 6 219271_at GALNT14 UDP-N-acetyl-alpha-D- 4.506 0 0 galactosamine:polypeptide N- acetylgalactosaminyltransferase 14 (GalNAc- T14) 202269_x_at GBP1 guanylate binding protein 1, interferon-inducible, 4.495 0 0 67kDa 214463_x_at 4.484 0 0 219210_s_at RAB8B RAB8B, member RAS oncogene family 4.481 0 0 208962_s_at FADS1 fatty acid desaturase 1 4.479 0 0 209652_s_at PGF placental growth factor 4.472 0 0 202807_s_at TOM1 target of myb1 (chicken) 4.471 0 0 205488_at GZMA granzyme A (granzyme 1, cytotoxic T- 4.467 0 0 lymphocyte-associated serine esterase 3) 204014_at DUSP4 dual specificity phosphatase 4 4.466 0 0 210140_at CST7 cystatin F (leukocystatin) 4.466 0 0 210582_s_at LIMK2 LIM domain kinase 2 4.465 0 0 215553_x_at 4.464 0 0 208653_s_at CD164 CD164 molecule, sialomucin 4.449 0 0 205831_at CD2 CD2 molecule 4.445 0 0 209581_at PLA2G16 phospholipase A2, group XVI 4.444 0 0 212925_at C19orf21 chromosome 19 open reading frame 21 4.44 0 0 32029_at PDPK1 3-phosphoinositide dependent protein kinase-1 4.432 0 0 37831_at SIPA1L3 signal-induced proliferation-associated 1 like 3 4.426 0 0 209408_at KIF2C kinesin family member 2C 4.417 0 0 215349_at hCG_173047 hypothetical protein LOC643376 4.412 0 0 4 202632_at 4.406 0 0 202931_x_at BIN1 bridging integrator 1 4.401 0 0 222114_x_at 4.4 0 0 204170_s_at CKS2 CDC28 protein kinase regulatory subunit 2 4.398 0 0 205686_s_at CD86 CD86 molecule 4.396 0 0 202072_at HNRNPL heterogeneous nuclear ribonucleoprotein L 4.384 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 211981_at COL4A1 collagen, type IV, alpha 1 4.383 0 0 215049_x_at CD163 CD163 molecule 4.382 0 0 204430_s_at SLC2A5 solute carrier family 2 (facilitated 4.377 0 0 glucose/fructose transporter), member 5 220192_x_at SPDEF SAM pointed domain containing ets 4.364 0 0 transcription factor 208532_x_at KRTAP5-8 keratin associated protein 5-8 4.359 0 0 214253_s_at DTNB dystrobrevin, beta 4.353 0 0 206180_x_at ZNF747 zinc finger protein 747 4.35 0 0 206545_at CD28 CD28 molecule 4.342 0 0 204889_s_at NEURL neuralized homolog (Drosophila) 4.332 0 0 214542_x_at HIST1H2AI histone cluster 1, H2ai 4.325 0 0 200622_x_at CALM3 calmodulin 3 (phosphorylase kinase, delta) 4.324 0 0 221032_s_at TMPRSS5 transmembrane protease, serine 5 4.321 0 0 213466_at RAB40C RAB40C, member RAS oncogene family 4.299 0 0 218681_s_at SDF2L1 stromal cell-derived factor 2-like 1 4.297 0 0 221061_at PKD2L1 polycystic kidney disease 2-like 1 4.29 0 0 220140_s_at SNX11 sorting nexin 11 4.282 0 0 213223_at RPL28 ribosomal protein L28 4.271 0 0 218223_s_at PLEKHO1 pleckstrin homology domain containing, family 4.265 0 0 O member 1 202267_at LAMC2 laminin, gamma 2 4.257 0 0 201820_at KRT5 keratin 5 4.229 0.004 0 205367_at SH2B2 SH2B adaptor protein 2 4.224 0 0 59697_at RAB15 RAB15, member RAS onocogene family 4.203 0 0 212354_at SULF1 sulfatase 1 4.202 0 0 213647_at DNA2 DNA replication helicase 2 homolog (yeast) 4.194 0 0 213417_at TBX2 T-box 2 4.186 0 0 202705_at CCNB2 cyclin B2 4.183 0 0 218337_at FAM160B2 family with sequence similarity 160, member B2 4.183 0 0 220485_s_at SIRPG signal-regulatory protein gamma 4.174 0 0 211500_at MAPK11 mitogen-activated protein kinase 11 4.171 0 0 201720_s_at LAPTM5 lysosomal protein transmembrane 5 4.17 0 0 212737_at GM2A GM2 ganglioside activator 4.145 0 0 205695_at SDS serine dehydratase 4.142 0 0 218232_at C1QA complement component 1, q subcomponent, A 4.137 0 0 chain 205990_s_at WNT5A wingless-type MMTV integration site family, 4.12 0.002 0 member 5A 33307_at RRP7A ribosomal RNA processing 7 homolog A (S. 4.114 0 0 cerevisiae) 210797_s_at OASL 2'-5'-oligoadenylate synthetase-like 4.111 0 0 204269_at PIM2 pim-2 oncogene 4.104 0 0 221241_s_at BCL2L14 BCL2-like 14 (apoptosis facilitator) 4.098 0 0 206901_at C19orf57 chromosome 19 open reading frame 57 4.089 0 0 205098_at CCR1 chemokine (C-C motif) receptor 1 4.083 0 0 218368_s_at TNFRSF12A tumor necrosis factor receptor superfamily, 4.08 0 0 member 12A 202503_s_at KIAA0101 KIAA0101 4.068 0 0 212116_at TRIM27 tripartite motif-containing 27 4.058 0 0 219940_s_at PCID2 PCI domain containing 2 4.051 0 0 200628_s_at WARS tryptophanyl-tRNA synthetase 4.047 0 0 202627_s_at SERPINE1 serpin peptidase inhibitor, clade E (nexin, 4.046 0 0 plasminogen activator inhibitor type 1), member 1 219424_at EBI3 Epstein-Barr virus induced 3 4.046 0 0 219753_at STAG3 stromal antigen 3 4.038 0 0 202178_at PRKCZ protein kinase C, zeta 4.034 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 396_f_at EPOR erythropoietin receptor 4.028 0 0 209995_s_at TCL1A T-cell leukemia/lymphoma 1A 4.023 0 0 210847_x_at TNFRSF25 tumor necrosis factor receptor superfamily, 4.015 0 0 member 25 213425_at WNT5A wingless-type MMTV integration site family, 4.015 0.001 0 member 5A 209360_s_at RUNX1 runt-related transcription factor 1 4.014 0 0 214339_s_at MAP4K1 mitogen-activated protein kinase kinase kinase 4.008 0 0 kinase 1 217232_x_at HBB hemoglobin, beta 4.007 0.001 0 219634_at CHST11 carbohydrate (chondroitin 4) sulfotransferase 11 4.003 0 0 220358_at BATF3 basic leucine zipper transcription factor, ATF- 4.003 0 0 like 3 217516_x_at 4.002 0 0 207114_at LY6G6C lymphocyte antigen 6 complex, locus G6C 4.001 0 0 218439_s_at COMMD10 COMM domain containing 10 4 0 0 201617_x_at CALD1 caldesmon 1 0.249 0 0 207283_at RPL23AP32 ribosomal protein L23a pseudogene 32 0.249 0 0 207819_s_at ABCB4 ATP-binding cassette, sub-family B 0.249 0 0 (MDR/TAP), member 4 210374_x_at PTGER3 prostaglandin E receptor 3 (subtype EP3) 0.249 0 0 211276_at TCEAL2 transcription elongation factor A (SII)-like 2 0.249 0 0 203130_s_at KIF5C kinesin family member 5C 0.248 0 0 205551_at SV2B synaptic vesicle glycoprotein 2B 0.248 0 0 207016_s_at ALDH1A2 aldehyde dehydrogenase 1 family, member A2 0.248 0 0 209493_at PDZD2 PDZ domain containing 2 0.248 0 0 209905_at HOXA9 homeobox A9 0.248 0.003 0 204823_at NAV3 neuron navigator 3 0.247 0.001 0 213158_at 0.247 0 0 214690_at TAF1B TATA box binding protein (TBP)-associated 0.247 0 0 factor, RNA polymerase I, B, 63kDa 219543_at PBLD phenazine biosynthesis-like protein domain 0.246 0 0 containing 220065_at TNMD tenomodulin 0.246 0 0 221550_at COX15 COX15 homolog, cytochrome c oxidase 0.246 0 0 assembly protein (yeast) 202984_s_at BAG5 BCL2-associated athanogene 5 0.245 0 0 204849_at TCFL5 transcription factor-like 5 (basic helix-loop- 0.245 0 0 helix) 205931_s_at CREB5 cAMP responsive element binding protein 5 0.245 0 0 211721_s_at ZNF551 zinc finger protein 551 0.244 0 0 214700_x_at RIF1 RAP1 interacting factor homolog (yeast) 0.244 0 0 218602_s_at HAUS6 HAUS augmin-like complex, subunit 6 0.244 0 0 218820_at C14orf132 chromosome 14 open reading frame 132 0.244 0 0 221958_s_at GPR177 G protein-coupled receptor 177 0.244 0 0 36920_at MTM1 myotubularin 1 0.244 0 0 203187_at DOCK1 dedicator of cytokinesis 1 0.243 0 0 203427_at ASF1A ASF1 anti-silencing function 1 homolog A (S. 0.243 0 0 cerevisiae) 203680_at PRKAR2B protein kinase, cAMP-dependent, regulatory, 0.243 0 0 type II, beta 204342_at SLC25A24 solute carrier family 25 (mitochondrial carrier; 0.243 0 0 phosphate carrier), member 24 214814_at YTHDC1 YTH domain containing 1 0.243 0 0 217220_at 0.243 0 0 219093_at PID1 phosphotyrosine interaction domain containing 1 0.243 0 0 220170_at FHL5 four and a half LIM domains 5 0.243 0 0 204604_at PFTK1 PFTAIRE protein kinase 1 0.242 0 0 206519_x_at SIGLEC6 sialic acid binding Ig-like lectin 6 0.242 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 213549_at 0.242 0 0 218901_at PLSCR4 phospholipid scramblase 4 0.242 0 0 202011_at TJP1 tight junction protein 1 (zona occludens 1) 0.241 0 0 202436_s_at CYP1B1 cytochrome P450, family 1, subfamily B, 0.241 0.001 0 polypeptide 1 203563_at AFAP1 actin filament associated protein 1 0.241 0 0 204635_at RPS6KA5 ribosomal protein S6 kinase, 90kDa, polypeptide 0.241 0 0 5 205604_at HOXD9 homeobox D9 0.241 0 0 205887_x_at MSH3 mutS homolog 3 (E. coli) 0.241 0 0 205975_s_at HOXD1 homeobox D1 0.241 0 0 220770_s_at C5orf54 chromosome 5 open reading frame 54 0.241 0 0 222136_x_at ZNF43 zinc finger protein 43 0.241 0 0 212523_s_at KIAA0146 KIAA0146 0.24 0 0 206404_at FGF9 fibroblast growth factor 9 (glia-activating factor) 0.239 0 0 207417_s_at ZNF177 zinc finger protein 177 0.239 0 0 207547_s_at FAM107A family with sequence similarity 107, member A 0.239 0 0 213900_at FAM189A2 family with sequence similarity 189, member A2 0.239 0 0 214658_at 0.239 0 0 221683_s_at CEP290 centrosomal protein 290kDa 0.239 0 0 204457_s_at GAS1 growth arrest-specific 1 0.238 0 0 206737_at WNT11 wingless-type MMTV integration site family, 0.238 0 0 member 11 209355_s_at PPAP2B phosphatidic acid phosphatase type 2B 0.238 0.001 0 215718_s_at PHF3 PHD finger protein 3 0.238 0 0 220994_s_at STXBP6 syntaxin binding protein 6 (amisyn) 0.238 0 0 222129_at FAM134A family with sequence similarity 134, member A 0.238 0 0 222253_s_at POM121L9P POM121 membrane glycoprotein-like 9 (rat) 0.238 0 0 pseudogene 31799_at 0.238 0 0 215446_s_at LOX lysyl oxidase 0.237 0.001 0 220952_s_at PLEKHA5 pleckstrin homology domain containing, family 0.237 0 0 A member 5 210512_s_at VEGFA vascular endothelial growth factor A 0.236 0 0 216887_s_at LDB3 LIM domain binding 3 0.236 0 0 216983_s_at ZNF224 zinc finger protein 224 0.236 0 0 218911_at YEATS4 YEATS domain containing 4 0.236 0 0 220298_s_at SPATA6 spermatogenesis associated 6 0.236 0 0 202527_s_at SMAD4 SMAD family member 4 0.235 0 0 213221_s_at SIK2 salt-inducible kinase 2 0.235 0 0 218706_s_at GRAMD3 GRAM domain containing 3 0.235 0 0 221088_s_at PPP1R9A protein phosphatase 1, regulatory (inhibitor) 0.235 0 0 subunit 9A 204832_s_at BMPR1A bone morphogenetic protein receptor, type IA 0.234 0 0 210632_s_at SGCA sarcoglycan, alpha (50kDa dystrophin-associated 0.234 0 0 glycoprotein) 203021_at SLPI secretory leukocyte peptidase inhibitor 0.233 0.01 0.02 214862_x_at 0.233 0 0 219023_at C4orf16 chromosome 4 open reading frame 16 0.233 0 0 211355_x_at LEPR leptin receptor 0.232 0 0 214212_x_at FERMT2 fermitin family homolog 2 (Drosophila) 0.232 0 0 214920_at THSD7A thrombospondin, type I, domain containing 7A 0.232 0 0 205132_at ACTC1 actin, alpha, cardiac muscle 1 0.231 0.001 0 212731_at ANKRD46 ankyrin repeat domain 46 0.231 0 0 222111_at 0.231 0 0 209525_at HDGFRP3 hepatoma-derived growth factor, related protein 0.23 0 0 3

Downloaded From: https://jamanetwork.com/ on 10/01/2021 209993_at ABCB1 ATP-binding cassette, sub-family B 0.23 0 0 (MDR/TAP), member 1 215936_s_at KIAA1033 KIAA1033 0.23 0 0 205803_s_at TRPC1 transient receptor potential cation channel, 0.229 0 0 subfamily C, member 1 213183_s_at CDKN1C cyclin-dependent kinase inhibitor 1C (p57, Kip2) 0.229 0 0 217974_at TM7SF3 transmembrane 7 superfamily member 3 0.229 0 0 206070_s_at EPHA3 EPH receptor A3 0.228 0 0 212417_at SCAMP1 secretory carrier membrane protein 1 0.228 0 0 213800_at CFH complement factor H 0.228 0.001 0 215992_s_at RAPGEF2 Rap guanine nucleotide exchange factor (GEF) 2 0.228 0 0 203078_at CUL2 cullin 2 0.227 0 0 215046_at C2orf67 chromosome 2 open reading frame 67 0.227 0 0 219094_at ARMC8 armadillo repeat containing 8 0.227 0 0 220855_at 0.227 0 0 212637_s_at WWP1 WW domain containing E3 ubiquitin protein 0.226 0 0 ligase 1 217257_at SH3BP2 SH3-domain binding protein 2 0.226 0 0 201149_s_at TIMP3 TIMP metallopeptidase inhibitor 3 0.225 0 0 209101_at CTGF connective tissue growth factor 0.225 0.001 0 214359_s_at HSP90AB1 heat shock protein 90kDa alpha (cytosolic), class 0.225 0 0 B member 1 48031_r_at C5orf4 chromosome 5 open reading frame 4 0.225 0 0 203355_s_at PSD3 pleckstrin and Sec7 domain containing 3 0.224 0 0 203799_at CD302 CD302 molecule 0.224 0 0 210839_s_at ENPP2 ectonucleotide 0.224 0 0 pyrophosphatase/phosphodiesterase 2 203501_at PGCP plasma glutamate carboxypeptidase 0.223 0 0 202932_at YES1 v-yes-1 Yamaguchi sarcoma viral oncogene 0.222 0 0 homolog 1 214066_x_at NPR2 natriuretic peptide receptor B/guanylate cyclase 0.222 0 0 B (atrionatriuretic peptide receptor B) 215287_at 0.221 0 0 205880_at PRKD1 protein kinase D1 0.22 0 0 205923_at RELN reelin 0.22 0 0 211310_at EZH1 enhancer of zeste homolog 1 (Drosophila) 0.22 0 0 213317_at CLIC5 chloride intracellular channel 5 0.22 0 0 213839_at CLMN calmin (calponin-like, transmembrane) 0.22 0 0 219689_at SEMA3G sema domain, immunoglobulin domain (Ig), 0.22 0 0 short basic domain, secreted, (semaphorin) 3G 204688_at SGCE sarcoglycan, epsilon 0.219 0 0 208096_s_at COL21A1 collagen, type XXI, alpha 1 0.219 0 0 213610_s_at KLHL23 kelch-like 23 (Drosophila) 0.219 0 0 221016_s_at TCF7L1 transcription factor 7-like 1 (T-cell specific, 0.219 0 0 HMG-box) 204776_at THBS4 thrombospondin 4 0.218 0 0 215548_s_at SCFD1 sec1 family domain containing 1 0.218 0 0 217353_at 0.218 0 0 219777_at GIMAP6 GTPase, IMAP family member 6 0.218 0 0 203497_at MED1 mediator complex subunit 1 0.217 0 0 210665_at TFPI tissue factor pathway inhibitor (lipoprotein- 0.217 0 0 associated coagulation inhibitor) 211698_at EID1 EP300 interacting inhibitor of differentiation 1 0.217 0 0 221886_at DENND2A DENN/MADD domain containing 2A 0.217 0 0 217608_at SFRS12IP1 SFRS12-interacting protein 1 0.216 0 0 217936_at ARHGAP5 Rho GTPase activating protein 5 0.216 0 0 204963_at SSPN sarcospan (Kras oncogene-associated gene) 0.215 0 0 205403_at IL1R2 interleukin 1 receptor, type II 0.215 0 0 214678_x_at ZFX zinc finger protein, X-linked 0.215 0 0 © 2012 American Medical Association. All rights reserved.

Downloaded From: https://jamanetwork.com/ on 10/01/2021 203881_s_at DMD dystrophin 0.214 0 0 210247_at SYN2 synapsin II 0.214 0 0 215220_s_at TPR translocated promoter region (to activated MET 0.214 0 0 oncogene) 218613_at PSD3 pleckstrin and Sec7 domain containing 3 0.214 0 0 213895_at EMP1 epithelial membrane protein 1 0.213 0 0 216147_at 0.213 0 0 218218_at APPL2 adaptor protein, phosphotyrosine interaction, PH 0.213 0 0 domain and leucine zipper containing 2 220244_at LOH3CR2A loss of heterozygosity, 3, chromosomal region 2, 0.213 0 0 gene A 202973_x_at FAM13A family with sequence similarity 13, member A 0.212 0 0 204069_at MEIS1 Meis homeobox 1 0.212 0 0 210852_s_at AASS aminoadipate-semialdehyde synthase 0.212 0 0 214764_at RRP15 ribosomal RNA processing 15 homolog (S. 0.212 0 0 cerevisiae) 215095_at ESD esterase D/formylglutathione hydrolase 0.212 0 0 220319_s_at MYLIP myosin regulatory light chain interacting protein 0.212 0 0 37433_at PIAS2 protein inhibitor of activated STAT, 2 0.212 0 0 209023_s_at STAG2 stromal antigen 2 0.211 0 0 212188_at KCTD12 potassium channel tetramerisation domain 0.211 0 0 containing 12 220014_at PRR16 proline rich 16 0.211 0 0 221596_s_at C7orf64 chromosome 7 open reading frame 64 0.211 0 0 204273_at EDNRB endothelin receptor type B 0.21 0 0 206068_s_at ACADL acyl-Coenzyme A dehydrogenase, long chain 0.21 0 0 212193_s_at LARP1 La ribonucleoprotein domain family, member 1 0.21 0 0 213925_at C1orf95 chromosome 1 open reading frame 95 0.21 0 0 205911_at PTH1R parathyroid hormone 1 receptor 0.209 0 0 214044_at RYR2 ryanodine receptor 2 (cardiac) 0.209 0 0 215385_at 0.209 0 0 218967_s_at PTER phosphotriesterase related 0.209 0 0 206701_x_at EDNRB endothelin receptor type B 0.208 0 0 209700_x_at PDE4DIP phosphodiesterase 4D interacting protein 0.208 0 0 212709_at NUP160 nucleoporin 160kDa 0.208 0 0 202319_at SENP6 SUMO1/sentrin specific peptidase 6 0.207 0 0 203571_s_at C10orf116 chromosome 10 open reading frame 116 0.207 0 0 203705_s_at FZD7 frizzled homolog 7 (Drosophila) 0.207 0 0 204161_s_at ENPP4 ectonucleotide 0.207 0 0 pyrophosphatase/phosphodiesterase 4 (putative function) 219970_at GIPC2 GIPC PDZ domain containing family, member 2 0.207 0 0 204075_s_at KIAA0562 KIAA0562 0.206 0 0 213582_at ATP11A ATPase, class VI, type 11A 0.206 0 0 214295_at KIAA0485 hypothetical LOC57235 0.206 0 0 214761_at ZNF423 zinc finger protein 423 0.206 0 0 218236_s_at PRKD3 protein kinase D3 0.206 0 0 51158_at FAM174B family with sequence similarity 174, member B 0.206 0 0 204176_at KLHL20 kelch-like 20 (Drosophila) 0.205 0 0 209655_s_at TMEM47 transmembrane protein 47 0.205 0 0 212850_s_at LRP4 low density lipoprotein receptor-related protein 4 0.205 0 0 213845_at GRIK2 glutamate receptor, ionotropic, kainate 2 0.205 0 0 215054_at EPOR erythropoietin receptor 0.205 0 0 216035_x_at TCF7L2 transcription factor 7-like 2 (T-cell specific, 0.205 0 0 HMG-box) 219837_s_at CYTL1 cytokine-like 1 0.205 0 0 219999_at MAN2A2 mannosidase, alpha, class 2A, member 2 0.205 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 205051_s_at KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral 0.204 0 0 oncogene homolog 205352_at SERPINI1 serpin peptidase inhibitor, clade I (neuroserpin), 0.204 0 0 member 1 205532_s_at CDH6 cadherin 6, type 2, K-cadherin (fetal kidney) 0.204 0 0 219353_at NHLRC2 NHL repeat containing 2 0.204 0 0 221156_x_at CCPG1 cell cycle progression 1 0.204 0 0 203969_at PEX3 peroxisomal biogenesis factor 3 0.203 0 0 203991_s_at KDM6A lysine (K)-specific demethylase 6A 0.203 0 0 204364_s_at REEP1 receptor accessory protein 1 0.203 0.001 0 204840_s_at EEA1 early endosome antigen 1 0.203 0 0 213054_at HAUS5 HAUS augmin-like complex, subunit 5 0.203 0 0 204984_at GPC4 glypican 4 0.202 0 0 205330_at MN1 meningioma (disrupted in balanced 0.202 0 0 translocation) 1 205794_s_at NOVA1 neuro-oncological ventral antigen 1 0.202 0 0 206693_at IL7 interleukin 7 0.202 0 0 206950_at SCN9A sodium channel, voltage-gated, type IX, alpha 0.202 0 0 subunit 209632_at PPP2R3A protein phosphatase 2 (formerly 2A), regulatory 0.202 0 0 subunit B'', alpha 221841_s_at KLF4 Kruppel-like factor 4 (gut) 0.202 0 0 204602_at DKK1 dickkopf homolog 1 (Xenopus laevis) 0.201 0 0 213456_at SOSTDC1 sclerostin domain containing 1 0.201 0.003 0 213479_at NPTX2 neuronal pentraxin II 0.201 0 0 204442_x_at LTBP4 latent transforming growth factor beta binding 0.2 0 0 protein 4 207469_s_at PIR pirin (iron-binding nuclear protein) 0.2 0 0 217813_s_at SPIN1 spindlin 1 0.2 0 0 217894_at KCTD3 potassium channel tetramerisation domain 0.2 0 0 containing 3 220038_at 0.2 0 0 204606_at CCL21 chemokine (C-C motif) ligand 21 0.199 0 0 205083_at AOX1 aldehyde oxidase 1 0.199 0 0 219091_s_at MMRN2 multimerin 2 0.199 0 0 204731_at TGFBR3 transforming growth factor, beta receptor III 0.198 0 0 206101_at ECM2 extracellular matrix protein 2, female organ and 0.198 0 0 adipocyte specific 210791_s_at RICS Rho GTPase-activating protein 0.198 0 0 206355_at GNAL guanine nucleotide binding protein (G protein), 0.197 0 0 alpha activating activity polypeptide, olfactory type 208733_at RAB2A RAB2A, member RAS oncogene family 0.197 0 0 219372_at IFT81 intraflagellar transport 81 homolog 0.197 0 0 (Chlamydomonas) 219911_s_at SLCO4A1 solute carrier organic anion transporter family, 0.197 0 0 member 4A1 203354_s_at PSD3 pleckstrin and Sec7 domain containing 3 0.195 0 0 204284_at PPP1R3C protein phosphatase 1, regulatory (inhibitor) 0.195 0 0 subunit 3C 204422_s_at FGF2 fibroblast growth factor 2 (basic) 0.195 0 0 204732_s_at TRIM23 tripartite motif-containing 23 0.195 0 0 211372_s_at IL1R2 interleukin 1 receptor, type II 0.195 0 0 214790_at SENP6 SUMO1/sentrin specific peptidase 6 0.195 0 0 204894_s_at AOC3 amine oxidase, copper containing 3 (vascular 0.194 0 0 adhesion protein 1) 208876_s_at PAK2 p21 protein (Cdc42/Rac)-activated kinase 2 0.194 0 0 214265_at ITGA8 integrin, alpha 8 0.194 0 0 219163_at ZNF562 zinc finger protein 562 0.194 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 204066_s_at AGAP1 ArfGAP with GTPase domain, ankyrin repeat 0.193 0 0 and PH domain 1 205413_at MPPED2 metallophosphoesterase domain containing 2 0.193 0 0 207496_at MS4A2 membrane-spanning 4-domains, subfamily A, 0.192 0 0 member 2 (Fc fragment of IgE, high affinity I, receptor for; beta polypeptide) 212373_at FEM1B fem-1 homolog b (C. elegans) 0.192 0 0 208920_at SRI sorcin 0.191 0 0 203628_at IGF1R insulin-like growth factor 1 receptor 0.19 0 0 205422_s_at ITGBL1 integrin, beta-like 1 (with EGF-like repeat 0.19 0 0 domains) 211379_x_at B3GALNT1 beta-1,3-N-acetylgalactosaminyltransferase 1 0.19 0 0 (globoside blood group) 204642_at S1PR1 sphingosine-1-phosphate receptor 1 0.189 0 0 204749_at NAP1L3 nucleosome assembly protein 1-like 3 0.189 0 0 205141_at ANG angiogenin, ribonuclease, RNase A family, 5 0.189 0 0 205907_s_at OMD osteomodulin 0.189 0 0 212843_at NCAM1 neural cell adhesion molecule 1 0.189 0 0 213290_at COL6A2 collagen, type VI, alpha 2 0.189 0 0 216037_x_at TCF7L2 transcription factor 7-like 2 (T-cell specific, 0.188 0 0 HMG-box) 203603_s_at ZEB2 zinc finger E-box binding homeobox 2 0.187 0 0 204742_s_at PDS5B PDS5, regulator of cohesion maintenance, 0.187 0 0 homolog B (S. cerevisiae) 208228_s_at FGFR2 fibroblast growth factor receptor 2 0.187 0 0 213943_at TWIST1 twist homolog 1 (Drosophila) 0.187 0 0 215388_s_at 0.187 0 0 204754_at HLF hepatic leukemia factor 0.186 0 0 215506_s_at DIRAS3 DIRAS family, GTP-binding RAS-like 3 0.186 0 0 201843_s_at EFEMP1 EGF-containing fibulin-like extracellular matrix 0.185 0 0 protein 1 204501_at NOV nephroblastoma overexpressed gene 0.185 0 0 205529_s_at RUNX1T1 runt-related transcription factor 1; translocated 0.185 0 0 to, 1 (cyclin D-related) 205632_s_at PIP5K1B phosphatidylinositol-4-phosphate 5-kinase, type 0.185 0 0 I, beta 212554_at CAP2 CAP, adenylate cyclase-associated protein, 2 0.185 0 0 (yeast) 216973_s_at HOXB7 homeobox B7 0.185 0 0 219892_at TM6SF1 transmembrane 6 superfamily member 1 0.185 0 0 220487_at SNTG2 syntrophin, gamma 2 0.185 0 0 204802_at RRAD Ras-related associated with diabetes 0.183 0 0 213267_at DOPEY1 dopey family member 1 0.183 0 0 213959_s_at RPGRIP1L RPGRIP1-like 0.183 0 0 218694_at ARMCX1 armadillo repeat containing, X-linked 1 0.183 0 0 218701_at LACTB2 lactamase, beta 2 0.183 0 0 219427_at FAT4 FAT tumor suppressor homolog 4 (Drosophila) 0.183 0 0 214136_at NUDT13 nudix (nucleoside diphosphate linked moiety X)- 0.182 0 0 type motif 13 204348_s_at AK3L1 adenylate kinase 3-like 1 0.181 0 0 205498_at GHR growth hormone receptor 0.181 0 0 205749_at CYP1A1 cytochrome P450, family 1, subfamily A, 0.181 0 0 polypeptide 1 219583_s_at SPATA7 spermatogenesis associated 7 0.181 0 0 202551_s_at CRIM1 cysteine rich transmembrane BMP regulator 1 0.18 0 0 (chordin-like) 210222_s_at RTN1 reticulon 1 0.18 0 0 212308_at CLASP2 cytoplasmic linker associated protein 2 0.18 0 0 214999_s_at RAB11FIP3 RAB11 family interacting protein 3 (class II) 0.18 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 203431_s_at RICS Rho GTPase-activating protein 0.179 0 0 217602_at PPIA peptidylprolyl isomerase A (cyclophilin A) 0.179 0 0 214890_s_at FAM149A family with sequence similarity 149, member A 0.178 0 0 220117_at ZNF385D zinc finger protein 385D 0.176 0 0 221760_at MAN1A1 mannosidase, alpha, class 1A, member 1 0.176 0 0 222072_at XPNPEP1 X-prolyl aminopeptidase (aminopeptidase P) 1, 0.176 0 0 soluble 211959_at IGFBP5 insulin-like growth factor binding protein 5 0.175 0.002 0 213791_at PENK proenkephalin 0.175 0 0 204557_s_at DZIP1 DAZ interacting protein 1 0.174 0 0 207943_x_at PLAGL1 pleiomorphic adenoma gene-like 1 0.173 0 0 202994_s_at FBLN1 fibulin 1 0.172 0 0 206726_at HPGDS hematopoietic prostaglandin D synthase 0.172 0 0 202350_s_at MATN2 matrilin 2 0.171 0 0 205158_at RNASE4 ribonuclease, RNase A family, 4 0.171 0 0 214012_at ERAP1 endoplasmic reticulum aminopeptidase 1 0.171 0 0 218469_at GREM1 gremlin 1, cysteine knot superfamily, homolog 0.171 0 0 (Xenopus laevis) 212706_at RASA4 RAS p21 protein activator 4 0.17 0 0 213754_s_at PAIP1 poly(A) binding protein interacting protein 1 0.17 0 0 215635_at 0.17 0 0 218546_at C1orf115 chromosome 1 open reading frame 115 0.17 0 0 203178_at GATM glycine amidinotransferase (L-arginine:glycine 0.169 0 0 amidinotransferase) 210832_x_at PTGER3 prostaglandin E receptor 3 (subtype EP3) 0.169 0 0 220559_at EN1 engrailed homeobox 1 0.169 0 0 213032_at NFIB nuclear factor I/B 0.168 0 0 214077_x_at MEIS3P1 Meis homeobox 3 pseudogene 1 0.168 0 0 217047_s_at FAM13A family with sequence similarity 13, member A 0.168 0 0 210306_at L3MBTL l(3)mbt-like (Drosophila) 0.167 0 0 203962_s_at NEBL nebulette 0.166 0 0 204793_at GPRASP1 G protein-coupled receptor associated sorting 0.166 0 0 protein 1 206271_at TLR3 toll-like receptor 3 0.166 0 0 212730_at SYNM synemin, intermediate filament protein 0.166 0 0 222164_at FGFR1 fibroblast growth factor receptor 1 0.166 0 0 204569_at ICK intestinal cell (MAK-like) kinase 0.165 0 0 206307_s_at FOXD1 forkhead box D1 0.165 0 0 213661_at PAMR1 peptidase domain containing associated with 0.165 0.001 0 muscle regeneration 1 218959_at HOXC10 homeobox C10 0.165 0 0 201540_at FHL1 four and a half LIM domains 1 0.164 0 0 203717_at DPP4 dipeptidyl-peptidase 4 0.164 0 0 212915_at PDZRN3 PDZ domain containing ring finger 3 0.164 0 0 213547_at CAND2 cullin-associated and neddylation-dissociated 2 0.164 0 0 (putative) 219407_s_at LAMC3 laminin, gamma 3 0.164 0 0 215059_at 0.163 0 0 219747_at C4orf31 chromosome 4 open reading frame 31 0.163 0 0 208116_s_at MAN1A1 mannosidase, alpha, class 1A, member 1 0.162 0 0 210174_at NR5A2 nuclear receptor subfamily 5, group A, member 2 0.161 0 0 213684_s_at PDLIM5 PDZ and LIM domain 5 0.161 0 0 207955_at CCL27 chemokine (C-C motif) ligand 27 0.16 0 0 208015_at SMAD1 SMAD family member 1 0.16 0 0 213325_at PVRL3 poliovirus receptor-related 3 0.16 0 0 204940_at PLN phospholamban 0.159 0 0 213493_at SNED1 sushi, nidogen and EGF-like domains 1 0.159 0 0 214651_s_at HOXA9 homeobox A9 0.159 0.001 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 218625_at NRN1 neuritin 1 0.159 0 0 221796_at NTRK2 neurotrophic tyrosine kinase, receptor, type 2 0.159 0 0 202435_s_at CYP1B1 cytochrome P450, family 1, subfamily B, 0.158 0 0 polypeptide 1 202995_s_at FBLN1 fibulin 1 0.158 0 0 209994_s_at 0.158 0 0 222291_at FAM149A family with sequence similarity 149, member A 0.158 0 0 204686_at IRS1 insulin receptor substrate 1 0.157 0 0 205591_at OLFM1 olfactomedin 1 0.157 0 0 222270_at SMEK2 SMEK homolog 2, suppressor of mek1 0.157 0 0 (Dictyostelium) 203662_s_at TMOD1 tropomodulin 1 0.156 0 0 209758_s_at MFAP5 microfibrillar associated protein 5 0.156 0 0 210432_s_at SCN3A sodium channel, voltage-gated, type III, alpha 0.156 0 0 subunit 209343_at EFHD1 EF-hand domain family, member D1 0.155 0 0 221207_s_at NBEA neurobeachin 0.155 0 0 204482_at CLDN5 claudin 5 0.154 0 0 204873_at PEX1 peroxisomal biogenesis factor 1 0.154 0 0 211548_s_at HPGD hydroxyprostaglandin dehydrogenase 15-(NAD) 0.154 0 0 203222_s_at TLE1 transducin-like enhancer of split 1 (E(sp1) 0.153 0 0 homolog, Drosophila) 218712_at C1orf109 chromosome 1 open reading frame 109 0.152 0 0 203343_at UGDH UDP-glucose dehydrogenase 0.151 0 0 207735_at RNF125 ring finger protein 125 0.151 0 0 219274_at TSPAN12 tetraspanin 12 0.151 0 0 220159_at ABCA11P ATP-binding cassette, sub-family A (ABC1), 0.151 0 0 member 11 (pseudogene) 203706_s_at FZD7 frizzled homolog 7 (Drosophila) 0.15 0 0 203961_at NEBL nebulette 0.15 0 0 205226_at PDGFRL platelet-derived growth factor receptor-like 0.15 0 0 202920_at ANK2 ankyrin 2, neuronal 0.149 0 0 205987_at CD1C CD1c molecule 0.149 0 0 204201_s_at PTPN13 protein tyrosine phosphatase, non-receptor type 0.148 0 0 13 (APO-1/CD95 (Fas)-associated phosphatase) 210258_at RGS13 regulator of G-protein signaling 13 0.148 0 0 217643_x_at 0.148 0 0 219778_at ZFPM2 zinc finger protein, multitype 2 0.148 0 0 207002_s_at PLAGL1 pleiomorphic adenoma gene-like 1 0.146 0 0 213930_at 0.146 0 0 220484_at MCOLN3 mucolipin 3 0.146 0 0 201787_at FBLN1 fibulin 1 0.145 0 0 209821_at IL33 interleukin 33 0.145 0 0 221014_s_at RAB33B RAB33B, member RAS oncogene family 0.145 0 0 203735_x_at PPFIBP1 PTPRF interacting protein, binding protein 1 0.144 0 0 (liprin beta 1) 203951_at CNN1 calponin 1, basic, smooth muscle 0.144 0 0 220351_at CCRL1 chemokine (C-C motif) receptor-like 1 0.144 0 0 220576_at PGAP1 post-GPI attachment to proteins 1 0.144 0 0 205200_at CLEC3B C-type lectin domain family 3, member B 0.143 0 0 215073_s_at NR2F2 nuclear receptor subfamily 2, group F, member 2 0.143 0 0 205381_at LRRC17 leucine rich repeat containing 17 0.142 0 0 209292_at ID4 inhibitor of DNA binding 4, dominant negative 0.142 0 0 helix-loop-helix protein 202422_s_at ACSL4 acyl-CoA synthetase long-chain family member 0.141 0 0 4 203485_at RTN1 reticulon 1 0.141 0 0 206631_at PTGER2 prostaglandin E receptor 2 (subtype EP2), 53kDa 0.141 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 221350_at HOXC8 homeobox C8 0.141 0 0 204869_at PCSK2 proprotein convertase subtilisin/kexin type 2 0.14 0 0 205898_at CX3CR1 chemokine (C-X3-C motif) receptor 1 0.14 0 0 211248_s_at CHRD chordin 0.14 0 0 213802_at 0.14 0 0 221921_s_at CADM3 cell adhesion molecule 3 0.14 0 0 210198_s_at PLP1 proteolipid protein 1 0.139 0 0 218311_at MAP4K3 mitogen-activated protein kinase kinase kinase 0.139 0 0 kinase 3 204010_s_at KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene 0.138 0 0 homolog 209616_s_at CES1 carboxylesterase 1 (monocyte/macrophage serine 0.138 0 0 esterase 1) 218326_s_at LGR4 leucine-rich repeat-containing G protein-coupled 0.138 0 0 receptor 4 218468_s_at GREM1 gremlin 1, cysteine knot superfamily, homolog 0.138 0 0 (Xenopus laevis) 203180_at ALDH1A3 aldehyde dehydrogenase 1 family, member A3 0.137 0 0 218513_at C4orf43 chromosome 4 open reading frame 43 0.137 0 0 201150_s_at TIMP3 TIMP metallopeptidase inhibitor 3 0.136 0 0 209242_at PEG3 paternally expressed 3 0.136 0 0 214705_at INADL InaD-like (Drosophila) 0.136 0 0 205421_at SLC22A3 solute carrier family 22 (extraneuronal 0.135 0 0 monoamine transporter), member 3 206960_at LPAR4 lysophosphatidic acid receptor 4 0.135 0 0 201525_at APOD apolipoprotein D 0.134 0 0 206432_at HAS2 hyaluronan synthase 2 0.134 0 0 214434_at HSPA12A heat shock 70kDa protein 12A 0.134 0 0 221974_at IPW imprinted in Prader-Willi syndrome (non-protein 0.134 0 0 coding) 205364_at ACOX2 acyl-Coenzyme A oxidase 2, branched chain 0.133 0 0 213397_x_at 0.133 0 0 214829_at AASS aminoadipate-semialdehyde synthase 0.133 0 0 215626_at 0.133 0 0 203763_at DYNC2LI1 dynein, cytoplasmic 2, light intermediate chain 1 0.132 0 0 204591_at CHL1 cell adhesion molecule with homology to 0.132 0 0 L1CAM (close homolog of L1) 213817_at IRAK3 interleukin-1 receptor-associated kinase 3 0.132 0 0 219902_at BHMT2 betaine-homocysteine methyltransferase 2 0.132 0 0 220356_at CORIN corin, serine peptidase 0.132 0 0 208131_s_at PTGIS prostaglandin I2 (prostacyclin) synthase 0.131 0 0 214823_at ZNF204 zinc finger protein 204 pseudogene 0.131 0 0 205063_at SIP1 survival of motor neuron protein interacting 0.13 0 0 protein 1 205620_at F10 coagulation factor X 0.13 0 0 210145_at PLA2G4A phospholipase A2, group IVA (cytosolic, 0.13 0 0 calcium-dependent) 206167_s_at ARHGAP6 Rho GTPase activating protein 6 0.129 0 0 210078_s_at KCNAB1 potassium voltage-gated channel, shaker-related 0.129 0 0 subfamily, beta member 1 203789_s_at SEMA3C sema domain, immunoglobulin domain (Ig), 0.128 0 0 short basic domain, secreted, (semaphorin) 3C 205876_at LIFR leukemia inhibitory factor receptor alpha 0.128 0 0 209119_x_at NR2F2 nuclear receptor subfamily 2, group F, member 2 0.128 0 0 210375_at PTGER3 prostaglandin E receptor 3 (subtype EP3) 0.128 0 0 211734_s_at FCER1A Fc fragment of IgE, high affinity I, receptor for; 0.128 0 0 alpha polypeptide 222308_x_at 0.128 0 0 200884_at CKB creatine kinase, brain 0.127 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 205399_at DCLK1 doublecortin-like kinase 1 0.127 0 0 213894_at THSD7A thrombospondin, type I, domain containing 7A 0.127 0 0 205792_at WISP2 WNT1 inducible signaling pathway protein 2 0.126 0 0 213150_at HOXA10 homeobox A10 0.126 0 0 202242_at TSPAN7 tetraspanin 7 0.124 0 0 202274_at ACTG2 actin, gamma 2, smooth muscle, enteric 0.123 0.006 0.01 205440_s_at NPY1R neuropeptide Y receptor Y1 0.123 0 0 220512_at DLC1 deleted in liver cancer 1 0.123 0 0 222161_at NAALAD2 N-acetylated alpha-linked acidic dipeptidase 2 0.123 0 0 219578_s_at CPEB1 cytoplasmic polyadenylation element binding 0.122 0 0 protein 1 202342_s_at TRIM2 tripartite motif-containing 2 0.121 0 0 213247_at SVEP1 sushi, von Willebrand factor type A, EGF and 0.121 0 0 pentraxin domain containing 1 203762_s_at DYNC2LI1 dynein, cytoplasmic 2, light intermediate chain 1 0.12 0 0 207078_at MED6 mediator complex subunit 6 0.119 0 0 219825_at CYP26B1 cytochrome P450, family 26, subfamily B, 0.119 0 0 polypeptide 1 204237_at GULP1 GULP, engulfment adaptor PTB domain 0.118 0 0 containing 1 205280_at GLRB glycine receptor, beta 0.118 0 0 203896_s_at PLCB4 phospholipase C, beta 4 0.117 0 0 205366_s_at HOXB6 homeobox B6 0.117 0 0 209914_s_at NRXN1 neurexin 1 0.117 0 0 210549_s_at CCL23 chemokine (C-C motif) ligand 23 0.117 0 0 204154_at CDO1 cysteine dioxygenase, type I 0.116 0 0 220794_at GREM2 gremlin 2, cysteine knot superfamily, homolog 0.116 0 0 (Xenopus laevis) 206910_x_at CFHR2 complement factor H-related 2 0.114 0 0 209469_at GPM6A glycoprotein M6A 0.114 0 0 207317_s_at CASQ2 calsequestrin 2 (cardiac muscle) 0.113 0 0 218692_at GOLSYN Golgi-localized protein 0.113 0 0 203543_s_at KLF9 Kruppel-like factor 9 0.111 0 0 201147_s_at TIMP3 TIMP metallopeptidase inhibitor 3 0.109 0 0 205110_s_at FGF13 fibroblast growth factor 13 0.109 0 0 205191_at RP2 retinitis pigmentosa 2 (X-linked recessive) 0.109 0 0 203424_s_at IGFBP5 insulin-like growth factor binding protein 5 0.108 0 0 220327_at VGLL3 vestigial like 3 (Drosophila) 0.108 0 0 206876_at SIM1 single-minded homolog 1 (Drosophila) 0.105 0 0 219132_at PELI2 pellino homolog 2 (Drosophila) 0.105 0 0 203002_at AMOTL2 angiomotin like 2 0.104 0 0 210239_at IRX5 iroquois homeobox 5 0.103 0 0 215321_at RUNDC3B RUN domain containing 3B 0.103 0 0 205392_s_at 0.102 0 0 205978_at KL klotho 0.102 0 0 213922_at TTBK2 tau tubulin kinase 2 0.1 0 0 205549_at PCP4 Purkinje cell protein 4 0.099 0 0 203407_at PPL periplakin 0.098 0 0 220783_at MMP27 matrix metallopeptidase 27 0.098 0 0 207255_at LEPR leptin receptor 0.097 0 0 209074_s_at FAM107A family with sequence similarity 107, member A 0.095 0 0 212713_at MFAP4 microfibrillar-associated protein 4 0.094 0 0 217504_at ABCA6 ATP-binding cassette, sub-family A (ABC1), 0.094 0 0 member 6 205259_at NR3C2 nuclear receptor subfamily 3, group C, member 2 0.093 0 0 219064_at ITIH5 inter-alpha (globulin) inhibitor H5 0.093 0 0 205174_s_at QPCT glutaminyl-peptide cyclotransferase 0.092 0 0 213469_at PGAP1 post-GPI attachment to proteins 1 0.092 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 222288_at 0.092 0 0 209869_at ADRA2A adrenergic, alpha-2A-, receptor 0.091 0 0 219230_at TMEM100 transmembrane protein 100 0.091 0 0 219871_at FLJ13197 hypothetical FLJ13197 0.09 0 0 221646_s_at ZDHHC11 zinc finger, DHHC-type containing 11 0.09 0 0 201496_x_at MYH11 myosin, heavy chain 11, smooth muscle 0.089 0 0 203895_at PLCB4 phospholipase C, beta 4 0.089 0 0 204939_s_at PLN phospholamban 0.089 0 0 214954_at SUSD5 sushi domain containing 5 0.089 0 0 219602_s_at FAM38B family with sequence similarity 38, member B 0.089 0 0 207177_at PTGFR prostaglandin F receptor (FP) 0.088 0 0 204584_at L1CAM L1 cell adhesion molecule 0.087 0 0 213929_at EXPH5 exophilin 5 0.086 0 0 213451_x_at 0.085 0 0 215028_at SEMA6A sema domain, transmembrane domain (TM), and 0.085 0 0 cytoplasmic domain, (semaphorin) 6A 205728_at 0.084 0 0 216333_x_at 0.084 0 0 205612_at MMRN1 multimerin 1 0.083 0 0 202992_at C7 complement component 7 0.082 0 0 204235_s_at GULP1 GULP, engulfment adaptor PTB domain 0.082 0 0 containing 1 205528_s_at RUNX1T1 runt-related transcription factor 1; translocated 0.082 0 0 to, 1 (cyclin D-related) 205139_s_at UST uronyl-2-sulfotransferase 0.08 0 0 206093_x_at 0.08 0 0 205752_s_at GSTM5 glutathione S-transferase mu 5 0.078 0 0 213880_at LGR5 leucine-rich repeat-containing G protein-coupled 0.078 0 0 receptor 5 210299_s_at FHL1 four and a half LIM domains 1 0.076 0 0 205382_s_at CFD complement factor D (adipsin) 0.072 0 0 205407_at RECK reversion-inducing-cysteine-rich protein with 0.072 0 0 kazal motifs 206306_at RYR3 ryanodine receptor 3 0.07 0 0 206201_s_at MEOX2 mesenchyme homeobox 2 0.069 0 0 215303_at DCLK1 doublecortin-like kinase 1 0.068 0 0 214321_at NOV nephroblastoma overexpressed gene 0.067 0 0 219949_at LRRC2 leucine rich repeat containing 2 0.066 0 0 201497_x_at MYH11 myosin, heavy chain 11, smooth muscle 0.064 0 0 205187_at SMAD5 SMAD family member 5 0.064 0 0 205908_s_at OMD osteomodulin 0.062 0 0 219895_at FAM70A family with sequence similarity 70, member A 0.062 0 0 220276_at RERGL RERG/RAS-like 0.062 0 0 221950_at EMX2 empty spiracles homeobox 2 0.06 0 0 212762_s_at TCF7L2 transcription factor 7-like 2 (T-cell specific, 0.059 0 0 HMG-box) 205048_s_at PSPH phosphoserine phosphatase 0.057 0 0 213568_at OSR2 odd-skipped related 2 (Drosophila) 0.057 0 0 207961_x_at MYH11 myosin, heavy chain 11, smooth muscle 0.053 0 0 204846_at CP ceruloplasmin (ferroxidase) 0.05 0 0 206858_s_at HOXC6 homeobox C6 0.049 0 0 204719_at ABCA8 ATP-binding cassette, sub-family A (ABC1), 0.048 0 0 member 8 210096_at CYP4B1 cytochrome P450, family 4, subfamily B, 0.048 0 0 polypeptide 1 206227_at CILP cartilage intermediate layer protein, nucleotide 0.046 0 0 pyrophosphohydrolase 219908_at DKK2 dickkopf homolog 2 (Xenopus laevis) 0.045 0 0

Downloaded From: https://jamanetwork.com/ on 10/01/2021 213764_s_at MFAP5 microfibrillar associated protein 5 0.044 0 0 213765_at MFAP5 microfibrillar associated protein 5 0.044 0 0 205357_s_at AGTR1 angiotensin II receptor, type 1 0.043 0 0 218087_s_at SORBS1 sorbin and SH3 domain containing 1 0.042 0 0 207302_at SGCG sarcoglycan, gamma (35kDa dystrophin- 0.036 0 0 associated glycoprotein) 209614_at ADH1B alcohol dehydrogenase 1B (class I), beta 0.036 0 0 polypeptide 219295_s_at PCOLCE2 procollagen C-endopeptidase enhancer 2 0.036 0 0 208609_s_at TNXB tenascin XB 0.033 0 0 209763_at CHRDL1 chordin-like 1 0.033 0 0 214156_at MYRIP myosin VIIA and Rab interacting protein 0.033 0 0 203296_s_at ATP1A2 ATPase, Na+/K+ transporting, alpha 2 (+) 0.03 0 0 polypeptide 222043_at CLU clusterin 0.03 0 0 205883_at ZBTB16 zinc finger and BTB domain containing 16 0.028 0 0 203824_at TSPAN8 tetraspanin 8 0.025 0 0 206898_at CDH19 cadherin 19, type 2 0.025 0 0 209894_at LEPR leptin receptor 0.025 0 0 209612_s_at ADH1B alcohol dehydrogenase 1B (class I), beta 0.012 0 0 polypeptide 204712_at WIF1 WNT inhibitory factor 1 0.006 0 0 209613_s_at ADH1B alcohol dehydrogenase 1B (class I), beta 0.006 0 0 polypeptide

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eTABLE 3. Endotoxin assay of tumor supernatants

Sample Optical Density1 LPS concentration (EU/ml) Positive control2 0.0830 ± 0.026 0.005 Tumor 1 -0.771 ± 0.042 <0.005 (LLOQ) Tumor 2 -0.794 ± 0.027 <0.005 (LLOQ) Tumor 3 -1.00 ± 0.11 <0.005 (LLOQ) Tumor 4 -1.23 ± 0.054 <0.005 (LLOQ) Tumor 5 -1.00 ± 0.054 <0.005 (LLOQ) (EU = endotoxin units, LLOQ = lower limit of quantification)

1. Blank corrected; presented as mean ± standard deviation 2. Lowest value of standard curve

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