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Development and Characterization of Dehydrated Peanut–Cowpea Milk Powder for Use As a Dairy Milk Substitute in Chocolate Manufacture

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Development and Characterization of Dehydrated Peanut–Cowpea Milk Powder for Use As a Dairy Milk Substitute in Chocolate Manufacture Food Research International 43 (2010) 79–85 Contents lists available at ScienceDirect Food Research International journal homepage: www.elsevier.com/locate/foodres Development and characterization of dehydrated peanut–cowpea milk powder for use as a dairy milk substitute in chocolate manufacture Herta Aidoo a, Esther Sakyi-Dawson b, Kwaku Tano-Debrah b, Firibu Kwesi Saalia b,* a Cocoa Processing Company Ltd., Private Mail Bag, Tema, Ghana b Department of Nutrition and Food Science, P.O. Box LG 134, University of Ghana, Legon, Accra, Ghana article info abstract Article history: This study explored the feasibility of producing peanut–cowpea milk for use in vegetable milk chocolates. Received 11 March 2009 Development of the vegetable milk followed a 3 Â 2 factorial design, with peanut–cowpea ratio (1:1, 1:2 Accepted 25 August 2009 and 1:3), and treatment with enzyme (i.e. enzyme hydrolyzed and non-hydrolyzed milk) as the factors. The milk was dehydrated and then milled using a hammer mill (mesh size 40). It was then used in recipes to produce chocolates and evaluated sensorially based on ranking for preference. Skimmed milk powder Keywords: was used to produce the control chocolate. The ratio of cowpea to peanut affected the chemical and func- Peanut–cowpea milk tional characteristics of the vegetable milk. Vegetable milk made from 1:2 ratios of peanuts:cowpea pro- Chocolate duced the most preferred chocolates. The successful application of this by industry will improve the Functional properties Friedman test utilization of the legume crops and enhance their market value. Ó 2009 Elsevier Ltd. All rights reserved. 1. Introduction using peanuts and cowpeas as sources of nutrients such as protein, dietary fibre, folate and other vitamins and minerals would be an The production of vegetable ‘‘milk” using legumes and oil seeds ideal dairy milk substitute. Vegetable milk made from peanut is an old technology that dates back to the 13th century (Smith & and cowpea blends could be dehydrated to produce an inexpensive Circle, 1972). In time the technology has been improved to include dry milk powder. Indeed Chandrasekhara et al. (1964) reported development of vegetable alternatives to dairy milk especially in spray drying of peanut milk combined with other sources of milk, the formulation of infant foods because they are high in protein, and the powder could be reconstituted in water. The objective of minerals and vitamins. The major legumes that have been used this study was to develop a dehydrated peanut–cowpea ‘‘milk” in vegetable milk production include soybean, cowpea, winged that would be acceptable for the production of milk chocolate. bean, groundnut and melon seeds or ‘‘agushie” (Nelson, Steinberg, & Wei, 1976; Senayah, 1993). 2. Materials and methods Current renewed interest in vegetable milk and products made using it is because of the growing awareness of the nutritional ben- 2.1. Raw materials efits of plant-based foods by health conscious consumers (Diarra, Nong, & Jie, 2005). Consequently soy and peanut milk have been The most common variety of peanuts (Arachis hypogaea), locally used in a variety of milk-based products including coffee creamers called ‘Chinese’ variety, and cowpeas (Vigna unguiculata), were (Mulando & Resurreccion, 2006), and chocolate milk drink purchased from the local market in Tema, Ghana. Paddy rice (Oryza (Deshpande, Chinnan, & Phillips, 2008). Legumes and oil seeds sativa) to be sprouted for use as a source of crude amylase for have characteristics that make it convenient to combine two or starch hydrolysis was obtained from the Irrigation Development more to obtain an acceptable product. In particular, peanut milk Authority, Ashaiman, Ghana. The legume grains and the paddy rice is known to be high in energy, whereas cowpea milk on its own were all sorted to remove moldy or discolored ones as well as any has low energy (Akinyele & Abudu, 1990). Combining the two extraneous materials from the lot. effectively removed these limitations (Asiamah, 2005). Further- more, it has been demonstrated that cowpea milk has a strong 2.2. Sample pre-treatments beany flavour while peanut–cowpea composite blends have mini- mal beany flavour (Nadutey, 1999). A composite product made Weighed quantities of the peanuts were blanched by submerg- ing in boiling water for 1 min, drained and then dehulled. To fur- * Corresponding author. ther ensure the removal of beany flavour in the final product, E-mail address: [email protected] (F.K. Saalia). and to help soften the peanuts, the dehulled nuts were steeped 0963-9969/$ - see front matter Ó 2009 Elsevier Ltd. All rights reserved. doi:10.1016/j.foodres.2009.08.018 80 H. Aidoo et al. / Food Research International 43 (2010) 79–85 in 2% NaHCO3 for 18 h, and then washed in clean water. Similarly, milk obtained from procedure 1. One (1) kilogram of sorted and weighed samples of cowpeas were steeped in water for about cleaned paddy rice was weighed and steeped in 3 l of clean water 5 min and dehulled. The dehulled beans were then steeped in 2% for 12 h. It was drained and spread on wet, heat sterilized (by boil- NaHCO3 for 3 h, and then washed in clean water (Asiamah, 2005). ing) jute sacks. The grains were covered by a piece of jute sack and left to stand at ambient temperature (24–28 °C) for 4 days. During 2.3. Preparation of the dehydrated legume milk the germination period the set up was periodically (twice a day) sprinkled with water to keep it moist. The sprouted rice was har- 2.3.1. Design of experiment vested, de-vegetated and dried in an air oven (Precision Thelco A3Â 2 full factorial design was followed, and the factors were; ovens, Model 160 M, USA) set at 50 °C for 24 h. The dried malt (a) the ratio of peanut–cowpea (3:1, 2:1, 1:1) and (b) the applica- was milled into powder using a hammer mill (Christy and Norris, tion of enzyme (crude amylase) (i.e. enzyme treated and non- Chemsford, England) equipped with mesh size 40. To obtain the enzyme treated) vegetable milk. enzyme, ice cold distilled water was added to weighed amounts Two different procedures were followed for making the vegeta- of the milled rice malt and allowed to stand for 30 min with inter- ble milk as illustrated in the process flow chart in Fig. 1. mittent stirring. The slurry was centrifuged in a refrigerated centri- fuge (4 °C) (TOMY Refrigerated Centrifuge – CX 250, Tokyo, Japan) at 8500g for 10 min. The supernatant, containing the crude enzyme 2.3.1.1. Procedure 1. The dehulled peanuts and cowpeas were com- was then filtered cold (8 °C) and stored in the refrigerator until it bined in specific ratios according to the experimental design and was used. The crude enzyme extract was mixed with part of the slurried in a blender using a ratio of one part of grains to two parts homogenized milk from procedure 1(8% v/v) and held at 56 °C of water. The slurry was further milled using a colloid mill (Premier for 45 min. The enzyme treated milk was then dehydrated on the 84, Premier Colloid Mills Ltd., Walton-on-Thames, Surrey, UK) to drum dryer. obtain a smooth, fine, homogenized milk. The milk was then dried From the two procedures six (6) different samples with using a drum dryer (R. Simon Dryers Ltd., Nottingham, England) peanut:cowpea ratios of 1:1, 2:1. 3:1 were obtained. operated at 40psi steam. The dehydrated milk flakes was milled to a fine powder with hammer mill equipped with a screen of mesh size 40. 2.4. Proximate analyses of the dehydrated peanut–cowpea ‘‘milk” 2.3.1.2. Procedure 2. This procedure involved the addition of crude Moisture content of the samples was determined by drying at amylase enzyme extracted from the germinated paddy rice, on the 103 ± 2 °C overnight as per the air oven drying method (AOAC, Paddy Rice Peanut ` Cowpea Soak (24 hrs) Dehull `Dehull Germinate (4 days) Soak in 2% Soak in 2% NaHCO3 (18hrs) NaHCO3 (3hrs) Oven dry Mill Flour Mix Peanut and Cowpea Add ice cold water (3:1, 2:1, 1:1) With stirring Water (meal: water- 1:2) Slurry Slurry Centrifuge (8,500g / 10mins@ 4ºC) Homogenize with Colloid Mill Filter Liquid Milk Enzyme Extract Dehydrate with Drum Dryer (Enzyme addition (8%v/v)/45mins) @56°C Mill into powder Final Powdered Product Fig. 1. Process flow chart for dehydrated peanut–cowpea milk. H. Aidoo et al. / Food Research International 43 (2010) 79–85 81 1990). Ash content was determined using a Gallenkamp Muffle incubated at 35 °C for 48 ± 2h. The number of colonies were Furnace, England, (AOAC, 1990). Fat was determined in the soxhlet counted and recorded as colony forming units per gram of sample apparatus (AOAC, 1990) using petroleum ether as the solvent of (cfu/g). extraction. The macro Kjeldahl procedure based on the AOAC method (AOAC, 1990) was used for nitrogen and the protein con- 4.3. Yeasts and molds determination tent of samples was calculated using 6.25 as the conversion factor. Crude fibre content was determined based on the procedure laid Yeasts and mold population in the samples were determined by down in the Fertilizers and Feeding Stuffs (Amendment) Regula- plating on Malt Extract Agar (pH 5.4 Oxoid Ltd., Basingstoke, tions 1976 SI No. 840 of Pearson’s Chemical Analysis of Foods, Hampshire – England) and incubating at 25 °C for 5 days. The num- (Egan, Kirk, & Sawyer, 1981). ber of colonies developed were counted and recorded as colony forming units per gram of sample (cfu/g). 3. Mineral analyses 4.4. Coliforms determination (presumptive test) The wet digestion method (AOAC, 1990) was used to eliminate all organic matter before samples were analyzed for the various Lauryl Tryptose Broth (pH 6.8 from Oxoid Ltd., Basingstoke, minerals.
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