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Morphometric data for M. domestica used in this study. Body Hallux Head-Body Tail Relative Opossum Sex Age mass length length length Tail Length (g) (cm) (cm) (cm) Juvenile 0G 0 PR 0G 0 PR 0G 0 PR 0G 0 PR 0G ) PR 89.0±23.4 0.5±0.8 15.3±1.6 8.2±1.04 0.54±0.04 Adult 2S 0 \U 2S ) \U 2S ) \U 2S ) \U 2S 0 \U 2S 0 \U 101±25.9 0.5±0.05 15.6±1.1 8.0±0.9 0.5±0.04 ,QGLYLGXDOVXVHGLQRXUSUHYLRXVVWXG\ VHH5XSHUWHWDO 9DOXHVLQEROGDUHPHDQ VG 5HODWLYHWDLOOHQJWKLVFDOFXODWHGDVWDLOOHQJWKGLYLGHGE\KHDGERG\OHQJWK Muscle Tissue Preparation &DXGDOPXVFOHWLVVXHZDVVDPSOHGIURPWKHWDLOVLPPHGLDWHO\SRVWPRUWHP%ULHIO\WKH WDLOV ZHUH GLYLGHG LQWR µSUR[LPDO¶ µWUDQVLWLRQDO¶ DQG µGLVWDO¶ UHJLRQV IRU PXVFOH GLVVHFWLRQ +D]LPLKDOLV HW DO 5XSHUW HW DO 7LVVXH EORFNV IURP WKH )&/ PXVFOH IURP HDFK WDLO UHJLRQ ZHUH KDUYHVWHG VWRUHG DW q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a PJPOZLWKJHOVDPSOHEXIIHUFRQWDLQLQJP07ULV S+ JO\FHUROP0 '77 6'6 DQG EURPRSKHQRO EOXH 0L]XQR\D HW DO 6DPSOHV ZHUH WKHQKHDWHG & IRUPLQDQGHLWKHUORDGHGLQJHOVRUVWRUHGDW&XQWLODQDO\VLV SDS-PAGE and Densitometry 0+&LVRIRUPVZLOOEHVHSDUDWHGXVLQJHVWDEOLVKHGPHWKRGV 7DOPDGJHDQG5R\ SHUIRUPHG ZLWK VOLJKW PRGLILFDWLRQV 0L]XQR\D HW DO 5XSHUW HW DO 7KH DFU\ODPLGHN,N’±PHWK\OHQHELVDFU\ODPLGH %LV UDWLR RI WKH JHOV ZLOO EH ZLWK WKH WRWDODFU\ODPLGHSHUFHQWDJHHTXDOLQJDQGLQWKHVHSDUDWLQJJHO JO\FHURO DQG VWDFNLQJJHO JO\FHURO UHVSHFWLYHO\7KHEDVLFIRUPXODWLRQRIWKHHOHFWURGHEXIIHU ZLOO EH P0 7ULV S+ P0 JO\FLQH DQG 6'6 XSSHU EXIIHU ; 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