Adaptation of Aspergillus Niger to Multiple Agents Whose Action Mechanisms Are Different
Jpn. J. Med. Mycol. Vol. 36, 19-24, 1995 ISSN 0916-4804 Original Article Adaptation of Aspergillus niger to Multiple Agents Whose Action Mechanisms are Different Hideaki Matsuoka1, Jong-Chul Park1, Yasuyuki Nemoto1, Satoru Yamada2, Weimin Jing3, Yuansong Chen3, Kosuke Takatori4, Hiroshi Kurata5 1Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, 2-24-16, Nakamachi, Koganei, Tokyo 184, Japan 2Research and Development Division, Bio-Liken Inc., 1-5-8, Iwamotocho, Chiyoda-ku, Tokyo 101, Japan 3Research and Development Division, Hidan Co. Ltd., 627, Hananoi, Kashiwa, Chiba 277, Japan 4Hatano Research Institute, Food & Drug Safety Center, 729-5, Ochiai, Hadano, Kanagawa 257, Japan 5The Tokyo Kembikyoin Foundation, 4-8-32, Kudanminami, Chiyoda-ku, Tokyo 102, Japan (Received: 9, August 1994. Accepted: 9, September 1994] Abstract Adaptation of Aspergillus niger to amphotericin B (AMPH) and two imidazoles (miconazole (MCZ) and ketoconazole (KCZ)) was observed at a single hypha level with a continuous measurement system. It was found that a test hypha adapted to MCZ or KCZ was also adapted to AMPH but that a hypha adapted to AMPH was not adapted either to MCZ or KCZ. These adaptation phenomena to respective agents did not occur after incubation in the medium supplemented with ergosterol. The cross adaptation phenomena were suspected to be due to modification of the synthesis pathway of ergosterol and related derivatives. Key words: adaptation, hyphal growth rate, Aspergillus niger, amphotericin B, miconazole, ketoconazole. rienced by many mycologists and microbiologists Introduction 5-9). The newly developed BCT system has dem- A single hypha-based microbioassay has recently onstrated those adaptation phenomena much more been proposed and applied to the evaluation of clearly by tracing the same hypha throughout.
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