ARTICLE 1 Tibouchina Tibouchina Coniella Diaporthales "&J +JM Sordariomycetes systematics Tibouchina Tibouchina . . IMA FUNGUS · VOLUME 3 · NO 1: 1–7 · NO 3 · VOLUME FUNGUS IMA et al. .//G( with the diseased bearing diseased leaves were collected at two bearing diseased leaves were collected Pilidiella T. granulosa granulosa T. and damaging diseases affecting gardens, and also in nurseries @ < ;# ( #B"EEJ granulosa ; > ? at the Universidade fungus were deposited in the herbarium ! B B"E ( = BF#= in the fungal culture collection at the Universidade Federal B EJ=K0FL ! # E EJ M & 0 ;# MATERIAL AND METHODS MATERIAL Isolates of Samples of young abnormal branches @ N0F ?%F ME&? ? N0F " K ? + " J EF MJF R & B7@ P 9 @ #B9P677/ % 677Q +;R N0FGR6QJ;0F Pilidiella tibouchinae >T@ . plants M & (LE 6 Chrysoporthe cubensis sp. nov. associated with foliage blight of blight with foliage associated sp. nov. T. granulosa T. Melastomataceae on ;L 6

tibouchinae tibouchinae Tibouchina granulosa Tibouchina However, very little is known about the diseases However, et al.//2 Cryphonectria cubensis EJ=K0FL!#E([<QR6VWGR/QFNM&0 M#!B N!GVRW/=///B ?@X=1;L Abstract: 'F!# + © 2012 International Mycological Association © 2012 International Mycological conditions: distribute and transmit the work, under the following are free to share - to copy, You Attribution: . 6 %E? Pilidiella (quaresmeira) in Brazil (quaresmeira) granulosa Z# #F =F Non-commercial:No derivative works:Any of the above conditions can be waived if you get For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Nothing in this license impairs or restricts the author’s moral rights. permission from the copyright holder. #&N0F for which the name we conclude that it represents a new species, Article info:J17>./6.XF1.7!./6.X(1RF./6. Here we clarify the identity of the fungus associated with # ## ' & = surprising for such a common plant in the neotropics, and \< 3'3345676.M there are only three records of fungi on this host in the USDA !9;./6/ O'P#+.//. granulosa T. Usually there is a high demand for young 133 and parks because of its spectacular violet or pink blossoms and parks because of its spectacular violet %& ' ( VOLUME 3 · NO. 1 Seixas glorytree, is a fast growing tree native that occurs in the Atlantic occurs in the glorytree, is a fast growing tree native that !" # \ 0# Tibouchina granulosa Melastomataceae Tibouchina INTRODUCTION 16/RR7Q3./6./G/6/6 #\" are known to be a limiting factor in nursery production of T. granulosa. & 1 Miranda et al.

JJM 6 RQJ ;0F."&J Slides containing fungal structures were mounted in 7//RR.QJ;0F+JM G/ &"&J2Let al677/+JM6!E J et al .//7 ' ?P?R./[# ARTICLE ''# of fungal structures and measurements, as well as preparation & (E; ' [\R6 ' [ %GG/ E Eet al.//V.//7F V 6=&%!' .]<?%F et al ./6/ J' (EFEet al.//7.R:EE '#0E"R@ # ;67W/ "&J !+JM#' + et al ./66 &FJ% RESULTS J66Q/RJ'# @&FJ%3 3<<#? F< 6W// "&J +JM ?XEet al.//2 '

Harknessia eucalypti AY720745 AY339313 Coniella australiensis

100 AY339320 75 AY339319 Coniella fragariae 72 AY339317 AY339316 EU301037 100 Pilidiella eucalyptorum AY339338 98 AY339343 Pilidiella macrospora

85 HQ264189 Pilidiella crousii

100 BECM2 90 Pilidiella tibouchinae BECM1 HQ166057 94 Pilidiella granati AY339342 GU062317 Pilidiella sp. 51 30 changes AY339346 65 AY339344 Pilidiella quercicola DQ914688 AY339348 Schizoparme straminea EU301051 AY339324 Pilidiella diplodiella AY339323 AY339333 AY339334 Pilidiella diplodiopsis AY339332

Fig. 1.&.2'R/<"&J' &5..7E"5/QG2;"5/7/2;E5/WR2&G/#6/// F<=bold # 'Harknessia eucalypti @ FW./W2R

2 IMA FUNGUS Pilidiella tibouchinae sp. nov.

Magnaporthe grisea AB026819 ARTICLE Gaeumannomyces graminis var. avenae AF362556 100 Coniochaetidium savoryi AY346276 Coniochaeta velutina EU999180 Family Calosphaeria pulchella AY761075 Ca 100 Togninia novae-zealandiae AY761081 100 Phaeoacremonium sphinctrophorum DQ173151 T 100 Asterosporium asterospermum AB553745 97 Asterosporium asterospermum AB553741 Mazzantia napelli AF408368 Diaporthe perjuncta AF408356 60 Diaporthe pustulata AF408358 88 88 70 Diaporthe padi AF408354 Diaporthe angelicae AY196781 Phaeocytostroma ambiguum FR748102 60 61 Stenocarpella macrospora DQ377934 D Stenocarpella maydis DQ377936 98 Phaeocytostroma sacchari FR748105 Diaporthe detrusa AF408349 Phaeocytostroma plurivorum FR748104 73 Phaeocytostroma megalosporum FR748103 10 changes 100 Valsa ceratosperma AF408386 Valsella adhaerens AF408388 V 78 Leucostoma niveum AF408367 80 Greeneria uvicola AF362570 Melanconiella spodiaea AF408370 M1 Endothiella gyrosa AF362555 89 Cryphonectria macrospora AF408340 Cr Cryphonectria nitschkei AF408341 96 Ophiovalsa betulae AF408375 Phragmoporthe conformis AF408377 G 100 58 63 Gnomonia setacea AF362563 100 Melanconis stilbostoma AF408374 Key to families: Melanconis marginalis AF408373 M2 Ca: Calosphaeriaceae 62 Melanconis alni AF362566 Cr: Cryphonectriaceae Wuestneia molokaiensis AF408390 93 D: Diaporthaceae Harknessia eucalypti AF408363 G: Gnomoniaceae Harknessia gibbosa EF110615 M: Melanconidiaceae 100 Pilidiella eucalyptorum EU754150 T: Togniniaceae Coniella australiensis AF408336 V: Valsaceae 82 Coniella fragariae AF362553 BECM2 Pilidiella tibouchinae 98 59 BECM1 Pilidiella tibouchinae 79 92 Schizoparme botrytidis AF408383 Pilidiella macrospora AY339292 Coniella musaiaensis AF408337 Schizoparme straminea AY339296 M3 57 Pilidiella granati AF408379 53 Pilidiella quercicola AY339293 Pilidiella diplodiopsis AY339287 Pilidiella diplodiella AY339286 Pilidiella diplodiopsis AY339288 Pilidiella sp. AY339295 57 Pilidiella castaneicola AF408378

Fig. 2.&6Q/'6//<+JM' &5R72E"5/R6R;"5/Q6W;E5/2.6&6/#6/// &# 'Magnaporthe grisea and Gaeumannomyces graminis#avenae@F/.VQ67F!GV.RRV#

& "&J &= ' =!6+JM ! . & !6&"&J † "&J .R < !6' 'R/6 Pilidiella crousii and Pilidiella granati & QV =# 27 † +JM R2 < #=#GVV 'Q2/

VOLUME 3 · NO. 1 3 Miranda et al. ARTICLE

Fig. 3. Pilidiella tibouchinaeA–D.+Tibouchina granulosaE.EF, G. Vertical section through H, I.EJ, K.E56/3

67/=# Etymology10 R2 # =# R7V Tibouchina !6Q/' Diagnosis: Similar to Pilidiella eucalyptorum but lacking !.(+JM!6 conidial germ slits, and similar to P. petrakioidea but lacking #'Pilidiella &&%!'# '#@ Type: Brazil: Minas Gerais: Viçosa, campus of the Universidade Federal de Viçosa, on leaves of Tibouchina granulosa, Q ? ./6/ B. C. Miranda B"E G622G M A pycnidial coelomycete was regularly associated with XEJP=./Q.WMX<=E(E diseased tissues on the samples collected at the two 6QR66E(E6QR6.5EJ6G6R7R " species in the genus Pilidiella0;†677G#0et @B"EG622GB"EG62221 al. .//2< "&J5>‹.Q6WW2>‹.Q6WWRX+JM5>‹.Q6WWV>‹.Q6WWWX Accordingly, a new species name is introduced below to &%!5>‹.Q6WWQ>‹.Q6WW7 accommodate the fungus occurring on T. granulosa. Other specimen examined: Brazil: Minas Gerais: Viçosa, campus of Pilidiella tibouchinae %E?;L9 the Universidade Federal de Viçosa, on leaves of T. granulosa, 6W Esp. nov. ?./6/B. C. MirandaB"EG6222 ??RVG77. !G

4 IMA FUNGUS Pilidiella tibouchinae sp. nov.

Description: Lesions## ! 9 ; ./6/ J# Myrtales, ARTICLE as adaxial straw-coloured necrotic spots, mostly appearing et al. .//G 62 near the leaf veins, becoming yellowish to greyish with a dark families, are known hosts of Schizoparmaceae ! 9 brown to dark purple border, irregularly shaped, coalescing ; ./6/ ! # and leading to necrosis and distortion of large parts of from Myrtaceae Acca, Blepharocalyx, Eucalyptus, Eugenia, X Heteropyxis, Myrcia, Syzygium), Lythraceae Lythrum, " † Punica, and CombretaceaeAnogeissus, Anogeissus, symptoms, a shortening of branch internodes, leaf distortion, Terminalia#0et al.//2!9;./6/ bud death, necrosis and die-back of young stems are also J67Q/0;†677GPilidiella as a # J # synonym of ConiellaP# # # Conidiomata pycnidial, +JM N0F N0F ' adaxial, subcuticular, solitary, globose to depressed globose, E et al. .//. Pilidiella is distinct 2.RMWR & WRM66.R ) from ConiellaPilidiella has two main morphological criteria brown textura angularis 6MG WM6. ) separating it from Coniella: the presence of conidia that X X conidiophores are hyaline when young becoming pale brown with age formed on a dense, basal, cushion-like aggregation of Coniella Eet al..//. hyaline cells, mostly reduced to conidiogenous cells, # 6R ' QM6R & GM2 ) 6R Coniella # 0 et al .//2 6M.= Conidiogenous cells enteroblastic, (&6 RM6/ & .MG ) P. tibouchinae has hyaline conidia that become smoky brown smooth, hyaline, with minute collarette, and covered in 16WF Conidia mostly broadly ellipsoidal, often somewhat the phylogenetic analysis place P. tibouchinae in the Pilidiella \## clade, distinct from Coniella ! 6M. E 6/M6G & VMQ ) 1 5 6W < combination of morphological and molecular data, we prefer base, hilum sometimes slightly protuberant, aseptate, hyaline to place this fungus in the genus Pilidiella. 0# when immature, becoming smoky-brown at maturity, smooth, several species in the group still need to be re-examined, as #!.Pilidiella eucalyptorum clusters in the Coniella clade, and C. musariensis clusters in the Pilidiella Culture characteristics1 ?%F (EF 6. ( P. tibouchinae are small when compared 1E=QV to the species of Schizoparmaceae treated by Sutton VX\X 67Q/ 0 ;† 677G # 0 et al .//2 mostly composed of immersed mycelium, aerial mycelium ? P. tibouchinae show some # similarity to that of P. eucalyptorum and P. petrakioidea. ?%F X However, conidia of P. tibouchinae lack conidial germ slits web-like white to grey olivaceous, sometimes with some small P. eucalyptorum cinnamon areas centrally, occasionally becoming powdery P. petrakioidea" towards the periphery, abundant olivaceous black fruit bodies WM6.)P. eucalyptorum.R3, ?%F3[(EF and has hyaline to pale smoky-brown conidia, whereas #X those of P. eucalyptorum (EF3 Furthermore, P. tibouchinae also differs from P. petrakioidea rounded apices in P. petrakioidea 1 67 !# Coniella have been described in DISCUSSION association with members of the Myrtaceae: C. australiensis, C. castaneicola, C. costae, C. fragariae, and C. minima. & Tibouchina granulosa clearly belongs to E Pilidiella the Coniella/Pilidiella-complex that has Schizoparme and Coniella, the conidial morphology of these species is Schizoparmaceae, DiaporthalesX ; also provided here for comparison with that of P. tibouchinae et al .//W ! Schizoparmaceae include several &6. species associated with foliar diseases, sometimes Pilidiella tibouchinae to be occurring as secondary invaders of plant tissues infected described on a host belonging to Melastomataceae, on which † ! et it appears to be associated with a rather serious foliar and al. 677W& !# Schizoparmaceae in association with members of the genus pathological status of the fungus on Tibouchina granulosa, Tibouchina, and a single doubtful record of a Coniella on and evaluating potential disease control measures are now another member of the Melastomataceae, Miconia serrulata '.

VOLUME 3 · NO. 1 5 Miranda et al.

ACKNOWLEDGEMENTS

L EJF # " ?†B?J=L N0F ' # ARTICLE

et al. ./66 et al .//2 et al .//2 et al. .//2 et al .//2 et al. 677G

REFERENCES & 0;†677G F67VW 0;†677G #0 #0 Rajeshkumar Firdousi et al 6772 J67V7 #F67QG #0 J67Q/

FPF#67QGFConiellaProceedings of the Koninklijke Nederlandse Akademie van Wetenschappen, C 8616.6M6.R F J 67VW E L (MB" Biologica 1316RM2. ------in older conidia + - K @ >T N† > J=L ? F J F J P=N N !? J P=> M E (L ./6/ J diversity within the Cladosporium cladosporioides complex - + - + + - - - + - Davidiellaceae, CapnodialesStudies in Mycology 6716M72 KE?L;#>+JN%et al.//G F \ 1F(@ "" Botanical Journal of the Linnean Society 1411G77M2GV E (L @ L J >F ; BJ @ .//2 ?1#.6 Studies in Mycology 50167M.. E (L @ >T ; >=? J ( P

Myrtales KN .//V Calonectria species and their Cylindrocladium 1##Studies in Mycology 551.6GM..V E(L@>TJFLNL ?> .//7 E= Teratosphaeria on 6W 67 0 RW Elongate-fusoid .Q Ellipsoid 6V uncommon + .G 0 .. 0 - .1/6 Globose to subglobose 6R Globoid to subgloboid 6R Ellipsoid .7 Ellipsoid EucalyptusPersoonia 221GQM2Q E (L J E+ P KN L F; @ E P @J N @ >T .//7 ( CapnodialesStudies in Mycology 6416WM2W E (L B @>? @ >T J ;F

species recorded from members of .//7Fungal BiodiversityEJ+?J 1‘ E#JM E+F;F>L>B#+0.//.F preliminary overview of the Diaporthales based on large subunit

and Pilidiella N0F'Mycologia 9416/6WM6/G6 N>E?;J+&(JE677GConiella costae##Myrcia tomentosa Coniella Mycological Research 9716.G2M6.GV !F[ 677V International Standard for Phytosanitary Measures. ;1 J " ( ( E# 6/M6G&VMQ) 6.M62R&VRMQ) 67M..&GRM23 7M6V&GM2R) 7M6/M6.M62&VMWMQ) 6/M6.M6RM67&2MRMV) VMWM6.M6GR&.RMGMR) .MQ&.MGR3 VRMWR&GRM2R) 6QGM.RM.7MG.R&6GM6VM./M.6R3 6R [# + QM7M6/M6.R&RMVMWMQ) WM7&.RMG) 67M.Q&WMWR) G. Fusoid to ellipsoid - - Dianese 6GM.7&.RMGR) WG Fusoid to falcate + - 0;†677G 7M6/M66M62&VMWMQM6/) 62 + - #0 ! N! ; F ./6/ ! # ?N1 J ? ? + F;J MJNFX133=#33 !!FFFEE+677W(== Coniella fragariaeRevista Árvore 21: G/WMG66 !JFJENBK?6772FConiella P. tibouchinae P. P. petrakioidea P. P. jambolana P. P. granati P. P.eucalyptorum P.eucalyptorum P. diplodiella P. Pilidiella crousii C. terminaliae C. minima C. macrospora C. fragariae C. delicata C. costae C. castanaeicola Coniella australiensis Species Size l:b rate Shape Appendage Germ slit Reference Table 1. E Table "Acta Botanica Indica 2216G2M6GR

6 IMA FUNGUS Pilidiella tibouchinae sp. nov.

P@J@#%FP@677Q? ;F!N!E+F.//WF# ARTICLE Mycoses 41: and biology of the Diaporthales. Mycoscience 4816GRM622 6QGM6Q7 J E 67V7 & Coniella, Anthasthoopa, and + JE E (L @ >T & & CyclodomellaCanadian Journal of Botany 471V/GMV/Q ? L![ ./66 Diaporthaceae associated with root and JE67Q/The Coelomycetes: fungi imperfecti with pycnidia, IMA Fungus 216GM.2 acervuli and stromata. K1 E ? + P .//. " cultivo de plantas arbóreas do Brasil B 6 2 0# MJNF3! N F# 133=#3 [J(1"( 3<./?./6/ 0 ;† &; 677G Coelomycetous Anamorphs with Appendage- B ; P ? 677/ ; bearing ConidiaL1?( N0F# 0>?#@>TB@>?!(PL CryptococcusJournal of Bacteriology 17212.GQM2.2V ?> E (L .//2 J Coniella L&> &+ >&> 677/F and Pilidiella ' ;0F Eucalyptus and VitisJFMycological Research 108: In1 " ?F @ NP J >> L &> PCR .QGMG/G Protocols: a guide to methods and applications1G6RMG..J ;;L67W/A Mycological Colour ChartK1E N1F( ?"

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