On Flax Flea Beetles
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Journal of Plant Protection Research ISSN 1427-4345 ORIGINAL ARTICLE The eff ect of diff erent seeding densities of linseed (Linum usitatissimum L.) on fl ax fl ea beetles (Coleoptera: Chrysomelidae) Jacek Twardowski1*, Michał Hurej1, Radosław Ścibior2, Andrzej Kotecki3 1 Department of Plant Protection, Wroclaw University of Environmental and Life Sciences, Grunwaldzki 24a, 50-363 Wroclaw, Poland 2 Department of Zoology, Animal Ecology and Wildlife Management, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland 3 Department of Crop Production, Wroclaw University of Environmental and Life Sciences, Grunwaldzki 24a, 50-363 Wroclaw, Poland Vol. 57, No. 2: 158–166, 2017 Abstract DOI: 10.1515/jppr-2017-0022 Linseed, one of the oldest cultivated crops, is again gaining in importance, mainly due to its nutritional benefi ts and biomedical applications. Th erefore, it is expected that herbivores will Received: February 9, 2017 also exist in greater abundance. Among them the fl ea beetle, Aphthona euphorbiae Schrank Accepted: June 5, 2017 and Longitarsus parvulus Paykull are considered to be serious pests of fl ax grown for fi bre and seeds in Europe. Th e aim of this study was to determine fl ax fl ea beetles’ abundance, spe- *Corresponding address: cies richness and seasonal dynamics on linseed grown at diff erent densities. It was expected [email protected] that linseed seeding density can signifi cantly aff ect fl ea beetle populations. Th e experiment was carried out in Lower Silesia, Poland, from 2011 to 2013. A genetically modifi ed type of linseed overproducing fl avonoids was used. Flea beetles and the damages they caused were determined on plants and also a sweep net was used for the collection of adult beetles. Dur- ing the three years of the study 15 species of fl ea beetles were identifi ed from oil fl ax plants, with A. euphorbiae and L. parvulus being dominant. In terms of the total catch, the tendency was for beetle numbers to decrease with increasing plant density. Flax fl ea beetles feeding on linseed plants, irrespective of plant density, had two peaks of abundance. Th e fi rst peak was lower and occurred in June, when plants were at the blooming stage. Th is peak was caused by overwintering adults who colonized crops in spring. Th e second, higher peak of abundance was recorded in the second half of July, when plants were at the ripening stage. Th is peak was formed by adults of the new generation. Each year, at the higher population peak of abun- dance, the fl ea beetles were most numerous on plants grown at the lowest density. Th ere was one period, lasting either from mid-May to the fi rst few days of June, or from the beginning of June to mid-June, during which the number of holes and damage on plants of each treat- ment were highest. During the three years of the study there were several cases of signifi cantly higher numbers of fl ea beetle feeding symptoms on plants grown at the lowest density as compared to the medium and highest densities. Key words: abundance, fl ax fl ea beetles, linseed, seeding density eff ect, species richness Introduction Linseed (Linum usitatissimum Linnaeus, 1753), one and composite materials (Shim et al. 2014; Zuk et al. of the oldest cultivated crops, continues to be widely 2015). Th e raw material of this oil is also used for the grown for oil, fi bre, and food (Jhala and Hall 2010; production of biodiesel fuels (Demirbas 2009). Because Heller et al. 2015). Linseed oil has commercial value as of linseed oil’s protective properties, it is recommend- a component of adhesives, paints and varnishes, plas- ed in the treatment of gastrointestinal tract diseases ticizers, inks, and linoleum. It is a precursor of nylon (e.g. chronic peptic ulcers, chronic inflammation of Jacek Twardowski et al.: The eff ect of diff erent seeding densities of linseed on fl ax fl ea beetles 159 mucous membranes) and respiratory tract diseases. lowest – 400 seeds (marked as 1), the medium – 600 Th e dietary properties of fl ax oil are also very impor- seeds (2) and the highest – 800 seeds (3). Th e experi- tant. It acts positively on peristalsis and metabolism, ment was designed as a split-plot with four replicates and prevents skin diseases. Flaxseed oil lowers blood for each sowing density. Aft er linseed emergence and cholesterol and glucose levels (Rodriguez-Leyva et al. again before harvest the number of plants per m2 was 2013). Linseed lignans inhibit cell proliferation and counted. growth, making them potential anticancer agents (Al- Th e number of fl ea beetles and the symptoms of phonse and Aluko 2015). Flaxseed is added to animal their damage (small shot-holes on leaves and dam- feed to improve animal reproductive performance and age on stems) were recorded on 50 whole, consecutive health (Turner et al. 2014). plants in the middle row of each plot during the fl ax Two species of fl ax fl ea beetles, Aphthona euphor- growing season. A total of six (2011–2012) or seven biae (Schrank, 1781) and Longitarsus parvulus (Paykull, (2013) observations of the presence of fl ea beetles on 1799) are considered to be serious, worldwide pests of plants were made during the research seasons, and re- fl ax grown for fi bre and seeds (Heller et al. 2015). Th ese spectively, 10 (2011), 11 (2012) and nine (2013) times two species occur together, but A. euphorbiae thrives the damage was evaluated. Every second week adults within a wider temperature and humidity range than were also collected with a sweep net (38 cm in diam- L. parvulus. Aphthona euphorbiae prefers warm, dry eter). A sample consisted of 10 180°-sweeps taken in weather which can be fatal to the pre-adult stages of the middle of each plot on each sampling date. Samples L. parvulus. Conversely, L. parvulus prefers cool, moist were placed individually in cardboard containers with conditions and is oft en the dominant species in cool, wet a paper towel saturated with 95% ethyl acetate. Sam- summers (Żurańska 1965a,b; Wise and Soroka 2003). ples were taken to the laboratory, where the insects As many as 14 species of fl ea beetles have been found on were hand separated from plant material and placed fl ax (Żurańska 1965b). Most of them are relatively rare in vials containing 75% ethyl alcohol. Samples were and are not known to cause noticeable crop damage. later sorted, and specimens were counted and identi- Th e aim of this study was to determine fl ax fl ea bee- fi ed. Chrysomelidae species names were adopted from tle abundance, species richness and seasonal dynamics Borowiec et al. (2011). on linseed grown at diff erent densities. It was expected Th e numbers of fl ea beetles and the symptoms of that linseed seeding density can infl uence fl ax fl ea bee- their damage found in diff erent treatments of the ex- tle populations. periment were compared for each date separately us- ing analysis of variance (ANOVA) followed by Tukey’s HSD (post-hoc). Statistical signifi cance was evaluated Materials and Methods at p ≤ 0.05. For statistical analysis soft ware Statistica, version 12 PL, was chosen. Th e experiment was conducted at the Experimental Research Station in Pawłowice, near Wrocław, Lower Results and Discussion Silesia, Poland (51°1737’N, 17°1176’E) during three growing seasons (2011–2013). A genetically modi- Plant density fi ed type of L. usitatissimum (cv. Linola) overproduc- ing fl avonoids (Zuk et al. 2011) was used. Plants were As shown in Table 1, higher seed sowing density caused, grown on 15 m2 plots (10 × 1.5 m, row spacing 30 cm) as expected, higher plant density. The mean emer- of sandy soil. A 0.3 m-wide space between the experi- gence capacity over three years ranged from 47.4% to mental plots was maintained mechanically as bare soil, 56.8%, and increased with the decreasing number of and the plots were weeded regularly by hand. Th e seeds sown seeds. Th e highest percentage of plant reduction were sown at three diff erent densities per 1 m2: the during the growing season (33.5%) occurred in the Table 1. Changes in linseed plant density during the growing season, Pawłowice, Poland, means from 2011–2013 Mean number of plants 2 Emergence Reduction during Number of seeds sown per 1 m capacity growing season per 1 m2 after before [%] [%] emergence harvest 400 227 163 56.8 28.2 600 299 205 49.8 31.4 800 379 252 47.4 33.5 160 Journal of Plant Protection Research 57 (2), 2017 Table 2. Total number of fl ea beetles and damage observed on linseed plants Sowing seed 2011 2012 2013 2011–2013 density beetles damage beetles damage beetles damage beetles damage 400 52 498 22 4,092 a* 354 17,060 a 428 21,650 600 63 412 18 3,564 b 345 14,457 b 426 18,433 800 45 389 27 2,978 c 284 13,896 b 356 17,263 Total 160 1,299 67 1,0634 983 45,413 1,210 57,346 *means followed by diff erent letters within columns are signifi cantly diff erent (ANOVA, p ≤ 0.05) treatment with the highest seed sowing density. Sow- of the insects from all the treatments (1–5.9%, 2–7.6%, ing 400, 600 and 800 seeds per m2 resulted in 163, 205 3–7.4%). Only a few specimens were found of the three and 252 plants before harvest, respectively.