Fish Protein Hydrolysate in Diets for Nile Tilapia Post-Larvae
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Fish protein hydrolysate in diets for Nile tilapia post-larvae Thiberio Carvalho da Silva(1), Joana D’Arc Mauricio Rocha(1), Pedro Moreira(1), Altevir Signor(1) and Wilson Rogerio Boscolo(1) (1)Universidade Estadual do Oeste do Paraná, Campus Toledo, Rua da Faculdade, no 2.550, CEP 85903-000 Toledo, PR, Brazil. E-mail: [email protected], [email protected], [email protected], [email protected], [email protected] Abstract – The objective of this work was to determine the apparent digestibility coefficient (ADC) of crude protein, crude energy, fat, and dry matter of fish protein hydrolysate (FPH), made of by-products of Nile tilapia (Oreochromis niloticus) and whole sardines (Cetengraulis edentulus), and to evaluate the productive performance and muscle fiber growth of Nile tilapia post-larvae. Two trials were conducted, the first one to determine the digestibility in 120 fingerlings (70.0±2.0 g), and the second one to evaluate the productive performance of 375 post-larvae, with three days of age, which were distributed in 25 aquaria with 30 L of useful volume. Five diets were prepared based on vegetable ingredients, to which fish were included at 0, 2, 4, 6, and 8% FPH. For the evaluation of muscle growth, eight fish of each experimental unit were used. The ADC values found were: 98.29% for dry matter; 99.28% for crude protein; and 99.13% for gross energy. The best zootechnical response for the productive performance resulted from the treatment with the inclusion of fish hydrolysate at 4.75%. The diets affected the frequency of the muscle fiber diameters, mainly the growth by hyperplasia. FPH can be efficiently used, and its inclusion at 4.75% is indicated in the diets for Nile tilapia in the post-larvae stage. Index terms: Cetengraulis edentulus, Oreochromis niloticus, digestibility, fish meal, nutrition, peptides, pisciculture. Hidrolisado proteico de peixe em dietas para pós-larvas de tilápia-do-nilo Resumo – O objetivo deste trabalho foi determinar o coeficiente de digestibilidade aparente (CDA) da proteína bruta, da energia bruta, da gordura e da matéria seca de um hidrolisado proteico de peixe (HPP), feito de resíduos de tilápia-do-nilo (Oreochromis niloticus) e sardinha inteira (Cetengraulis edentulus), e avaliar o desempenho produtivo e o crescimento das fibras musculares de pós-larvas de tilápia-do-nilo. Realizaram-se dois ensaios, o primeiro para a determinação da digestibilidade em 120 alevinos (70,0±2,0 g) e, o segundo, para avaliação do desempenho produtivo de 375 pós-larvas, com três dias de idade, distribuídas em 25 aquários (unidade experimental) com volume útil de 30 L. Cinco rações à base de ingredientes vegetais foram elaboradas, às quais se incluíram os peixes a 0, 2, 4, 6 e 8% de HPP. Para a avaliação do crescimento muscular, oito peixes de cada unidade experimental foram utilizados. Os valores de CDA encontrados foram: 98,29% para matéria seca; 99,28% para proteína bruta; e 99,13% para energia bruta. As melhores respostas zootécnicas quanto ao desempenho produtivo resultaram do tratamento com a inclusão do hidrolisado proteico a 4,75%. As dietas influenciaram a frequência do diâmetro das fibras musculares, principalmente o crescimento por hiperplasia. O HPP pode ser eficientemente utilizado, e sua inclusão a 4,75% é indicada em dietas de tilápia-do-nilo na fase de pós-larva. Termos para indexação: Cetengraulis edentulus, Oreochromis niloticus, digestibilidade, farinha de peixe, nutrição, peptídeos, piscicultura. Introduction To achieve satisfactory performance indices, it is recommended that, in the initial stage, part of the The production of larvae and fry in the quantity and dietary protein be of animal origin because of the quality to meet the growing demand of fish farming better profile of available amino acids and minerals depends on efficient solutions to problems in the (Galdioli et al., 2000; NRC, 2011). Therefore, fish meal rearing process. Feeding outstands as the main factor continues to be the main protein source in diets for among those responsible for failures in larviculture, most species of farmed fish because of the balanced (Phelps, 2010). amino acid profile and the composition of essential Pesq. agropec. bras., Brasília, v.52, n.7, p.485-492, jul. 2017 DOI: 10.1590/S0100-204X2017000700002 486 T.C. da Silva et al. fatty acids, digestible energy, vitamins, and minerals evaluate the productive performance and muscle fiber (Tacon & Metian, 2008). growth of Nile tilapia post-larvae. However, worldwide fish meal production has staganated by approximately 6–7 million tons in Materials and Methods recent years, and with the increasing demand, its value has increased, raising production costs in aquaculture Digestibility and zootechnical performance trials systems (Tacon & Metian, 2008; Merino et al., 2010). were carried out at the aquaculture and fish nutrition Therefore, several studies have been conducted on laboratory of Grupo de Estudos de Manejo na the total or partial replacement of fish meal in the Aquicultura (Gemaq), of Universidade Estadual do preparation of diets for aquatic organisms, in order Oeste do Paraná (Unioeste), Campus Toledo, in the to maintain the nutritional standards of the feeds municipality of Toledo, PR, Brazil. (Teixeira et al., 2006). The production of FPH consisted of 80% residues An alternative with great potential is the use of by- from Nile tilapia filleting (heads, viscera, scales, fins, products of the fish-processing industry, in the form vertebral column, and adhered tissues) and 20% whole of protein hydrolysate and fish of low-commercial sardine which were used to enhance the aroma and value. The hydrolysis process is defined as the protein taste of FPH, in order to improve the final product breakdown into peptides of various sizes. This attractiveness (Broggi, 2014). This raw material was breakdown can be achieved chemically (with acids or crushed in a CAF food processor , model Boca 5, bases), or biologically (with enzymes). The enzymatic 250-watt power and 30 kg h-1 capacity; then, a water process is more promising, as it generates a product volume equivalent to 20% of the mass value was with high functionality and nutritional value (Naylor added and homogenized. The mixture was placed for et al., 2009; Pasupuleti & Braun, 2010). approximately 5 min in a RW 20 mechanical stirrer The composition of a protein hydrolysate, produced (Ika Brasil Equipamentos Laboratoriais, Analíticos e from fish residue, may contain on average 78.75% Processos Ltda., Campinas, SP, Brazil). Subsequently, crude protein, 3.42% fat, and 12.51% ash (Nilsang et butylated hydroxytoluene (BHT) and butylated al., 2005). The partial replacement of the fish meal hydroxyanisole (BHA) were added at 0.01%. The with this product, even at low percentages, shows a temperature was adjusted to 60°C in a water bath to beneficial effect on the productive performance and promote the enzymatic activity. Alcalase enzyme was immunological activity of several fish species, mainly added at 0.5% and stirred continuously for 60 min. in the early stages of life (Refstie et al., 2004; Hevroy After this time, the enzymatic activity was stopped by et al., 2005). raising the temperature to 85°C for 15 min, and a food Tang et al. (2008) have shown that including up to preservative (citric acid) was added. At the end of the 10% fish protein hydrolysate (FPH) in the diet of yellow process, the liquid hydrolysate was filtered through a croaker (Pseudosciaena crocea) improved the growth 0.5 mm steel sieve to remove small bones. and immunological parameters of this fish. Likewise, To determine digestibility, the diets were elaborated Lian et al. (2005) and Chotikachinda et al. (2013) with an extruded practical feed (used as reference), found positive effects with FPH supplementation in and an extruded test feed composed of 80% of the the diet of the studied species. The results found in reference feed and 20% of the ingredient to be tested, these studies may be related to the high palatability adding 0.1% chromium oxide, used as inert marker of FPH, which contains biologically active, immune- (NRC, 2011) (Table 1). stimulating peptides with antibacterial properties For the feed production, the ingredients were that can be produced during the hydrolysis process initially milled in a hammer-type grinder with a 0.7 (Kotzamanis et al., 2007). mm sieve, and, then, weighed and mixed manually. The objective of this work was to determine the The crushed feed was moistened with 20% water, and apparent digestibility coefficient (ADC) of crude extruded in an ExMicro apparatus (Exteec Máquinas, protein, crude energy, fat, and dry matter of FPH, made Ribeirão Preto, SP, Brazil), with 10 kg h-1 production of by-products of Nile tilapia (Oreochromis niloticus) capacity. After this processing, the feeds were oven and whole sardines (Cetengraulis edentulus), and to dried at 55°C for 12 hours. Pesq. agropec. bras., Brasília, v.52, n.7, p.485-492, jul. 2017 DOI: 10.1590/S0100-204X2017000700002 Fish protein hydrolysate in diets for Nile tilapia 487 Nile tilapia juveniles (120) with a mean mass of CDA(n) = {100 -[100 ×(%Cr2O3d × %Cr2O3f) × (%Nf / 70±2 g were used. Fish were randomly distributed in %Nd)]}, in which: CDA (n) is the nutrient digestibility six 90 L conical tanks suitable for the collection of coefficient; %Cr2O3d is the percentage of chromium feces. The juveniles remained in the tanks for seven oxide in the diet; %Cr2O3f is the percentage of days in the tanks, in order to adapt to the diets and to chromium oxide in feces; %Nf is the percentage of the experimental conditions. The treatments, randomly nutrient in feces; %Nd is the percentage of nutrient in assigned at the beginning of the adaptive period, the diet. And CDA (i) = {CDAdt + [(CDAdt - CDAdr) consisted of three tanks with reference feed, and three × (0.8×Ddr / 0.2×Ding)]}, in which: CDA (i) is the tanks with test feed.