TOOLS AND RESOURCES Live cell imaging of meiosis in Arabidopsis thaliana Maria A Prusicki1, Emma M Keizer2†, Rik P van Rosmalen2†, Shinichiro Komaki1‡, Felix Seifert1§, Katja Mu¨ ller1, Erik Wijnker3, Christian Fleck2#*, Arp Schnittger1* 1Department of Developmental Biology, University of Hamburg, Hamburg, Germany; 2Department of Agrotechnology and Food Sciences, Wageningen University, Wageningen, The Netherlands; 3Department of Plant Science, Laboratory of Genetics, Wageningen University and Research, Wageningen, The Netherlands Abstract To follow the dynamics of meiosis in the model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes. Our method is based on the concomitant visualization of microtubules (MTs) and a meiotic cohesin subunit that allows following five cellular *For correspondence: parameters: cell shape, MT array, nucleus position, nucleolus position, and chromatin condensation.
[email protected] (CF);
[email protected] We find that the states of these parameters are not randomly associated and identify 11 cellular (AS) states, referred to as landmarks, which occur much more frequently than closely related ones, indicating that they are convergence points during meiotic progression. As a first application of our †These authors contributed system, we revisited a previously identified mutant in the meiotic A-type cyclin TARDY equally to this work ASYNCHRONOUS MEIOSIS (TAM). Our imaging system enabled us to reveal both qualitatively and Present address: ‡Plant Cell